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دراسة النمط المناعي وقابلية الاصابة بالعدوى الميكروبية لدى ذوي السمنة البالغين == Study The Immune Profile & Susceptibility to Some Microbial Infections in Obese Adults

اسم المؤلف: فؤاد قاسم جبير الزيادي
اسم المشرف: محمد عبد كاظم حسن السعدي | هديل فاضل فرهود
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: هدفت هذه الدراسة الى توضيح العلاقة بين البالغين الذين يعانون من السمنة والتعرض للعدوى الميكروبية من خلال تقدير المعايير المناعية مثل (IFN - γ & IL - 2)، عناصرcomplement system (C3, C4) , وقياس فعالية الخلايا المناعية الالتهامية من خلال الكشف عن الاصابة بـ Streptococcus pyogenes وMeasles virus. انجز هذا العمل على 50 شخصا من ذوي السمنة البالغين (11 ذكرا و39 انثى) تتراوح اعمارهم من (20 - 60) عاما قاموا بزيارة قسم التغذية في مستشفى مرجان التعليمي في محافظة بابل خلال الفترة من نوفمبر 2014 الى يناير 2015، و30 شخصا اخرين اصحاء لا يعانون من السمنة (21 ذكور و9 اناث) تتراوح اعمارهم من (22 - 42( عاما. وتم قياس مؤشر كتلة الجسم (BMI) لكل فرد من ذوي السمنة والاصحاء. تم جمع عينات الدم من كلا المجموعتين لقياس فعالية الخلايا الالتهامية، والمعايير المناعية الاخرى مثل (IFN - γ & IL - 2)، وعناصر complement system (C3, C4), والكشف عن الاصابة بـ Streptococcus pyogenes وMeasles virus بطريقة الامتزاز المناعي المرتبط بالانزيم. اظهرت النتائج ان تركيز (IL - 2) عند ذوي السمنة اقل بكثير وبشكل معنوي عن الذين لا يعانون من السمنة (P˂0.05) ، ولم يظهر تركيز IFN - γ)) في مجموعة ذوي السمنة انخفاضا معنويا (p>0.05) عن تركيزه عند الاصحاء. وكانت عناصر complement system (C3, C4)مرتفعة عند ذوي السمنة وبشكل عالي المعنوية (p˂0.001) عن مجموعة الاصحاء الذين لا يعانون من السمنة، ولكن فعالية الخلايا الالتهامية اظهرت انخفاضا عالي المعنوية (p˂0.001) عند مجموعة ذوي السمنة (6.1٪) مقارنة بمجموعة الاصحاء (10.6٪)، وزيادة تراكيز كل من ASO وanti - measles virus - IgG وبشكل عالي المعنوية (p˂0.001) عند ذوي السمنة مقارنة بالاصحاء | This study aimed to clarify the relationship between obese adults & susceptibility to microbial infections by evaluating the parameters of cytokines (IFN - γ & IL - 2), complement components (C3 & C4), and phagocytic index using of Anti - Streptococcus pyogenes Ab and Anti - Measles virus - IgG Ab as indicators of infections. This work was applied on 50 obese adults visited to the center of nutrition in Marjan hospital in Babylon province from November 2014 to January 2015, and 30 non - obese apparently healthy as controls. Obese adults consist of (11 males and 39 females) with age range 20 - 60 years, while 30 apparently health controls consist of (21 males and 9 females) with age range 22 - 50 years. Body mass index (BMI) for all subjects obese & controls was estimated. Blood samples were collected from obese group and control group to estimate phagocytic index, and ELISA test for cytokines (IFN - γ & IL - 2), complement components (C3 & C4), and detection of measles - IgG antibody and anti - streptolysin O antibody. IL - 2 concentration in obese adults was significantly lower than non - obese adults (p˂ 0.05), and no significant decreased (p>0.05) of IFN - γ in obese group, the complement components (C3 &C4) in obese adults were a highly significant than controls, but the phagocytic index showing there is a highly significant decreased (p˂0.001) in activity of neutrophils of obese adults (6.1%) compared to control group (10.6%), ASO and Anti - measles - IgG concentrations increased in obese adults with a highly significant (p˂0.001) as compared to controls. This findings can be concluded as that obese subjects have low levels of immune system and more susceptible to be infected with microbial infections

تقييم وتعريف المكورات المسبحية الطافرة والمتحولة اللثوية في مرضى تسوس الاسنان وامراض النسيج الداعم للاسنان == Evaluation and Identification of Streptococcus mutans and Entamoeba gingivalis in Patients with Periodontal Diseases and Dental Caries

اسم المؤلف: مؤيد نعمة مصلح الخفاجي
اسم المشرف: الهام عباس بنيان الساعدي | هيام خالص المسعودي
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: The present was carried out during the period of November 2014 to April 2015 in Al - Hilla City. This study included 100 samples which were collected from patients at ages ranged between 4 to 75 years old whom were suffering from dental caries and periodontal diseases ; (50) samples were taken from patients infected with dental caries (23 male and 27 female) and (50) samples taken from patients with periodontal diseases (15 male and 35 female), who have submitted to a college of Dentistry in Babylon university and special center of Dentistry. Thirty samples were taken from apparently healthy persons and considered as control.The isolation and identification of S. mutans was performed by Mitis Salivarius Bacitracin agar as a selective medium and biochemical tests as well as API 20 strep, then investigated the virulence factors and show the effect of some antibiotics on bacterial isolates, and the identification of E. gingivalis parasite was by a wet preparation method.The obtained results showed that S. mutans was with a higher existence in patients with dental caries, with lower existence in the patients with periodontal diseases and it was found in healthy persons (30%, 18%, and 0%) respectively. Entamoeba gingivalis was higher in patients with periodontal diseases than patient with dental caries and less in healthy individuals (62%, 24%, and 6%) respectively. The presence of these microorganisms was related to the type of dental disease (p=0. 009), gender (p=0. 009), age (p=0. 008) and risk factors (diabetes, smoking and hypertension) (p=0.037).The effect of some antibiotics on S.mutans was also tested, and the obtained results showed that all isolates were with high resistance to Erythromycin (70.8%) and (66.7%) were resistant to Doxycycline. Some isolates showed low level of resistance to Vancomycin (54.2%), CefatoximeII(33.4%) and Clindamycin (25%), while these isolates were with high susceptibility to Chloramphenicol (100%) and Ofloxacin (83.4%).Finally, some virulence factors of S. mutans were studied and it was seen that the (62.5%) of S. mutans isolates produced α - hemolysis and (8.3%) produced β - hemolysis but (29.2%) of isolates non - hemolysis, but (58.3%) of isolates were made capsule and (100 %) were able to synthesize glucan, (83.3%) of S. mutans isolates were able to produce mutacin and (45.83%) of isolates were found to produce dextranase enzyme, while (84%) were able to biofilm formation .In addition, all isolates in this study were able to adhere to oral epithelial cells.

دراسة على المايكرومايكروبية الشائعة المعزوله من القناة الهضمية وبعض المعايير المناعية للمرضى الذين يعانون من مرض السكري النوع الثاني == A Study on Common Microbiaes isolated from Gastrointestinal and some Immunological Parameter in Patients with Type II Diabeties Mellitus

اسم المؤلف: شذى خزعل كاظم الوائلي
اسم المشرف: هيام خالص المسعودي | علي حسين البياتي
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: المرضى الذين يعانون من من داء السكري النوع الثاني هم مجموعة غير متجانسة من الاضطرابات التي تتميز بمقاومة الانسولين الواسعة للانسجة ويختلف على نطاق واسع. داء السكري من النوع الثاني هي حتى الان الاكثر شيوعا. تضمنت الدراسة (100) مريض مصاب بالسكري النوع الثاني مشخص من قبل اطباء متخصصين بالسكري و(50) شخص من الناس الاصحاء وفي الفئه العمرية (25 - 65) سنة الوافدين الى مستشفيات في بابل (القاسم ومرجان) خلال الفترة من كانون الثاني 2015 حتى حزيران 2015.واظهرت النتائج ان البكتريا السالبه لصبغة كرام من مجموع العزلات هي السائدة مقارنة مع البكتريا الموجبه لصبغة كرام تشكل 26٪ وشكلت العزلات البكتيرية E.coli الاكثر شيوعا كانت يتبعها Enterobacter spp., K.Pneumoniae, Proteus spp., والبكتريا الاقل شيوعا كانتS.aureus, S.epidermidis, Citrobacter, Micrococcus Kocuria kristinae وStreptococcus spp,في حين ان الطفيليات الاكثر شيوعا كانت E.histolyticaبنسبة 55% وGiardia lambliaبنسبة 45% . تم دراسة عوامل الضراوة للبكتريا الشائعه المعزولة من مرضى السكري من النوع الثاني في هذه الدراسة. وكانت عزلات K.pneumoniae وعزلات Enterobacter وبعض عزلات E.coli تعطي الكبسول بينما بكتيريا Proteus spp. المعزوله في هذه الدراسة لم تمتلك الكبسولة. تم الكشف عن عامل الاستعمار CF - I وIII في العزلات البكتيرية فكانت ايجابيه في البكتريا الاكثر شيوعا وكانت جميع عزلات بكتيريا المكورات العنقودية الذهبية قادرة على انتاج المخثرة التي يعتبر عامل الضراوه لامراضية هذه البكتيريا. وان كل عزلات المكورات العنقودية الذهبية والاشريكية القولونية اظهرت تحللا كاملا (lyses كاملة) β - الانحلالي، في حين ان جميع عزلات المكورات العقدية نمط (lyses غير مكتملة) α - الانحلالي حيث اظهرت خط مخضر حول المستعمرات البكتيرية. وكانت جميع عزلات S.epidermidis وبعض العزلات من Proteus spp. (غير الحالة للدم) γ - الحالة للدم. في هذه الدراسة تم التحري عن sidrophore بين العزلات البكتيرية واظهرت النتائج ان جميع العزلات البكتيرية (E. coli K.Pneumoniae,)، ومعظمها من عزلات E. aerogenes و.Proteus spp كانوا قادرين على صنع sidrophore. وكانت بعض العزلات من. Proteus spp وEnterobacter ايجابية لتفاعل الجيلاتيناز، في حين ان جميع العزلات من Klebseilla واكثر عزلات E.coli سالبة لهذا العامل. واظهرت نتائج اختبار انتاج ال lipase في سالبة الجرام ان معظم العزلات ايجابية لاختبار lipase. وكشفت النتائج ان جميع العزلات كانت موجبه Biofilm وضع تشكيل (50٪)، في حين لا يوجد عزلات سالبه له. وفيما يتعلق بعوامل الضراوة الشائعة للبكتيريا المعزولة من عدم وجود مرض السكري وعوامل الضراوة فكانت نادرة. وكانت نتائج المتغيرات المناعية (C3، C4، Ig A، IL4، وIL6) للمرضى السكري في الدراسة, قد انخفضت مستويات C3، IgA، IL4، وIL6 للمرضى السكري ، في حين مستوى C4 قد ارتفع. من خلال نتائج هذه الدراسة، يمكن ان نستنتج؛ ان انواع البكتيريا التي عزلت من مرض السكري من النوع الثاني هي التالية E.coli تليها K. Pneumoniae, Enterobacter spp., Proteus spp, Micrococcus, Citrobacter S.epidermidis, kocuria kristinae, S.aureus , and Streptococcus spp. والطفيليات هي الاميبا الحاله للنسيج ثم الجيارديا اللامبيليا | In this study include (100) diabetic type (II) patients as diagnosed by Diabetolegist and 50 healthy control with an age range of (25 - 65 years) who attended to the two hospitals in Babylon : (Al - Qasem and Marjan), during the period from January 2015 until June 2015. Shown that grams - negative bacteria constitutes 74% from the total isolates and were considered the predominant aetiological agent of DM compared to gram - positive bacteria which constitutes 26%. The most common bacterial isolates were E.coli followed by Klebsella Pneumoniae, Enterobacter spp, Proteus spp, and less common bacteria were Staphylococcus aureus, Staphylococcus epidermidis, Kocuria kristinae, Citrobacter, Micrococcus and Streptococcus spp, whereas the most common parasites were Entamoeba histolytica 55% and Giardia lamblia 45%.The virulence factors of common bacterial isolated from diabetes Mellitus type II in this study are studied. The capsule was present in all Klebsella pneumoniae isolates, Enterobacter isolates and some isolates of E.coli, whereas the other bacteria such as Proteus mirabilis isolates in this study does not possess the capsule. Colonization factor antigens were detected; The CF - I and III were detected in mostly bacterial isolates which showed positive results with these factors. All isolates of Staphylococcus aureus bacteria were able to produce coagulase which is considered as a virulence factor for pathogenicity of these bacteria. All isolates of Staphylococcus aureus and E. coli expressed β - hemolytic (complete lyses), while all isolates of Streptococcus were α - hemolytic pattern (incomplete lyses) showing a greenish line around the bacterial colonies. All isolates of Staphylococcus epidermidis and some isolates of Proteus spp. were γ - hemolytic (non hemolytic). In the present study the siderophore synthesis was investigated among bacterial isolates and the results showed that all bacterial isolates (E. coli and K. pneumonia), and mostly of isolates of Enterobacter spp. and Proteus spp. were able to synthesis siderophore. Some isolates of Proteus mirabilis and Enterobacter spp. were positive for gelatinase reaction, while all isolates of Klebseilla pneumoniae and mostely E.coli were negative for this factor. The results of lipase production test in G - ve showed that most isolates were positive to lipase production test. The results revealed that all isolates were biofilm former, high and moderate biofilm formation mode were account for (50%) while there is no isolates that express non - biofilm formation. Regarding the virulence factors of common bacterial isolated of non - diabetes Mellitus have rare virulence factors.The results of Immunological parameters (C3, C4, IgA, IL4, and IL6) for DM patients were studied. In this study the levels of C3, IgA, IL4, and IL6 for DM patients were decreased, whereas the level of C4 was increased. In light of the results documented in this study, we can conclude the following; the types of bacteria which isolated from diabetes mellitus type II from the most to least common are E.coli followed by K. Pneumoniae, Enterobacter spp., Proteus spp., S.aureus, S.epidermidis, kocuria kristinae, Citrobacter, Micrococcus, and Streptococcus spp. And parasite : Entamoeba histolytica and Giardia lamblia isolated from diabetes mellitus type II. Most bacterial isolates in this study have the ability to possess more than one virulence factor such as capsule, siderophors, lipase production, hemolysin, extracellular protease, adherence factors, and biofilms to produce infection in diabetes mellitus type II patients. The virulence factors of common bacterial isolated in non - diabetes mellitus have rare virulence factor. The levels of Immunological parameters of C3, IgA, IL4, and IL6 for DM patients were decreased, whereas the level of C4 was increased.

دراسة لبعض الخصائص الوبائية والمصلية لاصابات الـBordetella perlussis في محافظة بابل

اسم المؤلف: عمار عباس شعلان
اسم المشرف: محمد عبود القرة غولي | يحيى الطفيلي
الموضوع العام: الطب
السنة: 2005
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: تهدف هذه الدراسة الى وصف بعض الخصائص الوبائية للسعال الديكي في محافظة بابل وتقييم الاضداد المتخصصة للـ Bordetella pertussis IgG وIgA باستخدام تقنية الـ ELISA للتشخيص المختبري للسعال الديكي، هذا بالاضافة الى قياس الاستجابة المناعية الخلطية للقاح الثلاثي (DPT). جمعت عينات الدم من : 68 طفل مصاب بالسعال ديكي راقد في المستشفى (36 غير ملقح و32 ملقح) ، 103 من اشخاص اسوياء (49 شخصا ملقحا، 54 شخصا غير ملقح) و20 من العاملين بالخدمة الصحية في محافظة بابل. اضداد Bordetella pertussis IgG وIgA تم قياسها بواسطة تقنية الـ ELISA لكل مجموعات الدراسة. التعداد الكلي والتفريقي للخلايا البيضاء اجري للـ68 مريض و20 من مجموعة السيطرة. اظهرت الدراسة ان اعلى مستوى حدوث للسعال الديكي لوحظ في المرضى اقل من عمر سنة (49% من مجموع الحالات). كانت نسبة الذكور للاناث 1.27 : 1. 71% من المرضى مقيمين في المناطق الريفية مقارنة بـ 29 % في المناطق الحظرية. 36 (53%) من المرضى كانوا غير ملقحين و26 (38%) كانوا ملقحين جزئيا، بينما 6 (9%) تلقوا تلقيحا كاملا (3 جرعات من اللقاح الثلاثي). لوحظت مضاعفات السعال الديكي في 88% من المرضى، والتي كانت بصورة رئيسية الاختناق (88%)، اختلاجات (26%)، ذات الرئة (29%)، والوفاة (4%). كما لوحظ ارتفاع في تعداد الخلايا البيضاء في 64% من المرضى وارتفاع في تعداد الخلايا اللمفاوية في 69% من المرضى. معظم الحالات سجلت خلال الفترة بين شهر نيسان وتموز. كانت الحساسية والتخصص التشخيصي لاضداد الـ Bordetella pertussis IgG في الاطفال غير الملقحين كانت 77% و100% على التوالي، في حين كانت الحساسية والتخصص التشخيصي لاضداد Bordetella pertussis IgG في الاطفال الملقحين 67%، 91% على التوالي. كانت الحساسية والتخصص التشخيصي لاضداد الـ Bordetella pertussis IgA في الاطفال غير الملقحين كانت 33% و100% على التوالي، في حين كانت الحساسية والتخصص التشخيصي لاضداد Bordetella pertussis IgA في الاطفال الملقحين 72%، 86% على التوالي. لوحظت افضل حساسية وتخصص تشخيصي في العيانات التي جمعت بعد اكثر من 21 يوما بعد ظهور اعراض السعال الديكي. تم الحصول على اعلى حساسية وتخصص تشخيصي (92، 82% على التوالي) باستعمال مستوى موجب لـ اضداد Bordetella pertussis IgG او IgA. اظهرت الدراسة ان متوسط مستوى اضداد Bordetella pertussis IgG في الاسوياء الملقحين كانت 56.41 U/ml (المدى 19.77 - 143.89 U/ml)، بينما بلغ متوسط اضداد Bordetella pertussis IgG في الاسوياء غير الملقحين 29.33 U/ml (المدى 1.01 - 56.9 U/ml). كان متوسط مستوى اضداد Bordetella pertussis IgA في الاسوياء الملقحين كانت 9.05 U/ml (المدى 3.6 - 29.74 U/ml)، بينما بلغ متوسط اضداد Bordetella pertussis IgA في الاسوياء غير الملقحين 4.79 U/ml (المدى 3.05 - 7 U/ml). اظهرت الدراسة ان 10.7 % (11/103) من الاسوياء يظهرون مستوى موجب لاضداد Bordetella pertussis IgG او IgA، في حين اظهر 70% (14/20) من العاملين في الخدمة الصحية في هذه الدراسة مستوى موجب لاضداد Bordetella pertussis IgG او IgA | The aims of this study is to describe some of the epidemiological features of pertussis in Babylon Province in Iraq, and to evaluate the specific anti Bordetella pertussis IgG and IgA ELISA technique in the laboratory diagnosis of pertussis, and to determine the humoral immune response to DTP vaccine. Blood samples were obtained from the following groups : 68 (36 unvaccinated and 32 vaccinated)clinically diagnosed hospitalized pertussis patients, 103 normal healthy subject of similar age groups (49 vaccinated and 54 unvaccinated) and 20 healthcare workers in Babylon Province. The anti Bordetella pertussis IgG and IgA antibodies were tested by ELIZA technique in all study groups. The study shows that the highest incidence of pertussis was noticed in patients less than 1 year (49% of the total pertussis patients). The male to female ratio was 1.27 : 1. There were 71% of pertussis cases from rural area compared with 29% from urban area. 36 (53%) of the patients were unvaccinated and 26 (38%) of the patients were partially vaccinated, while only 6 (9%) are fully vaccinated (3 doses of DTP vaccine). Complications were noticed in (88%) of the patients; they are mainly apnea (88%), seizure (26%), pneumonia (29%), and death (4%). Most cases were noticed between March and July. The anti Bordetella pertussis IgG diagnostic sensitivity and specificity in unvaccinated subjects were 77%, 100% respectively; while in vaccinated subjects they were 67%, 91% respectively. The anti Bordetella pertussis IgA diagnostic sensitivity and specificity in unvaccinated subjects were 33%, 100% respectively, while in vaccinated subjects they were 72%, 86% respectively. The best diagnostic sensitivity and specificity was observed in samples collected after > 21 days of the onset of pertussis symptoms. The higher diagnostic sensitivity and specificity were obtained by using anti Bordetella pertussis IgG or IgA positive levels. The study shows that the anti Bordetella pertussis IgG mean level in DPT vaccinated normal subjects was 56.41 U/ml (range 19.77 - 143.89 U/ml), while it was 29.33 U/ml (range 1.01 - 56.9 U/ml) in unvaccinated subjects. The anti Bordetella pertussis IgA mean level in vaccinated normal subjects was 9.05 U/ml (range 3.6 - 29.74 U/ml), while it was 4.79 U/ml (range 3.05 - 7 U/ml) in unvaccinated subjects. There is 10.7% (11/103) of the normal subjects which have a positive anti Bordetella pertussis IgG or IgA levels. In addition, 70% (14/20) of the healthcare workers in this study have a positive anti Bordetella pertussis IgG or IgA levels.

تقويم فعالية الخميرة Candida albicans من المسح المهبلية للنساء في حيوية النطف واحداث العقم

اسم المؤلف: رقية يحيى عبد الشهيد الطفيمي
اسم المشرف: زيدان خليف عمران المعموري | فارس ناجي عبود الهادي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: The present study aimed to identify some of the molecular dynamic qualities and the type of Candida albicans and determine its virulence factors using some of the phenotypic and molecular techniques and their impact on the semen and causing infertility.The collection of 173 swab of the vagina and the cervix was where 97 women from women with primary infertility, including 78 women with positive and 19 women with a scanning negative.kmaamlh control and 76 women with secondary infertility, including 49 with positive screening women and 27 women with a scanning negative.kmaamlh control, women who Rajan unit infertility in Babylon, a maternity hospital and children and private clinics in the province of Babylon.The use of agricultural methods to isolate and diagnose 127 isolation dating back to Candida vaginal as well as the use of CHROMO agar medium test and test tobacco Agar to distinguish between certain types of Candida, was detected on some virulence factors such as the composition of the Germ tube and spores Alclamadah and production of enzymes case of fat (Alphusfullaypez) and the formation of membrane It was vital to draw your DNA candidiasis and the use of PCR technique where the starters used by the general whiteners, especially for the purpose of diagnosis isolates of Candida albicans and genetic profiling of yeast Candida depending on the variation in the ITS region area. Showed isolate and diagnose types of eggs in women with infertility initial results of 48 women infected with C.albicans by (61.53%), while 30 women is infected Non - C.albicans by (38.46%) either in women with secondary infertility showed the 29 women infected with C.albicans by ((59.18% and 20 women is infected non - C.albican by (40.81%). the results showed the center CHROMO agar medium isolates of Candida species C.albicans was once a green color and isolates C.tropicalis with blue color and with C.krusei color pink and showed C.glabrata violet and C.parapsilosis that showed white.And C.albicans isolates showed C.albicans result is positive for the production of germ tube and spores Alclamadah as well as the center of tobacco Acar which they appear yeast C.albicans white colonies free of filamentous structures, a test of this kind without other types of Candidapositive test.. While the production of enzymes showed a case of fat test (Alphusfullaypez) as a result of the positive isolates C.albicans and C.krusei The results showed that the incidence of candidiasis in women with infertility first was more ratio than the unemployed in secondary infertility. It was the incidence of type C.albicans is the largest in all of the women with primary infertility and secondary infertility women initial rate of 61.53% and 59.18%, respectively, followed by injury type C.glabrata by 17.9% in women with infertility first and 18.36% in secondary infertility. Distributed cases of four age groups and was the highest rates (47.91% (in women with infertility and the first (55.17%) in the women's secondary infertility is located within the age group (30 - 21) years old. In the case of a period of infertility and distributed cases into three periods and was the highest rate of infection is the period (5.1), where the percentage (81.81%) in infertile women was less than the rate of infection is the period (15 - 11), where the ratio of (6.49%) in infertile women. The results showed that the rate of infection C.albicans were higher in women with and who not to say on treatment by (65.33%) of either women with and who took up treatment (53.84%). As well as the incidence is higher in women infertile is Mentzmat appeared menstrual cycle by (63.75%) The women infertile women with regular menstrual cycle and to have a lower rate (55.31%). The incidence of rural women by (67.56%) higher than the women of the city that are represented (58.49%).It was three concentrations preparation 3×107, 5× 107, 7×108cfu/ml of all isolated from C.albicans and raw leach ate Khmer and observe their effect on semen and then finding the value of MED50 effective dose)) concentrations C.albicans way to draw a dose - of responsiveness was found that the concentration curve (107× 5 cfu/ml) top influential focus on movement and Sperm Viability. It was the influence of the C.albicans test on semen Concentration 107× 5cfu/ml over time (after 30 - 60 minutes) and a leaky yeast and after the screening device Alkazh Computer aided sperm analysis (CASA) where he found the impact of the C.albicans on sperm movement, Rapid progressive (a), Slow progressive (b) and Sperm Viability (V) over time after the text of the clock and watch.The pair initiator year ITS4 - ITS5 in the diagnosis of all isolates representing the total isolates succeeded as a return to form of the genus Candida spp. Where the results of the polymerization 520 bp. The pair initiator Specialist in the diagnosis of Candida yeasts belonging to the type C.albicans and succeeded when he polymerization products 344bp, while the rest of the species is C.albicans did not show any polymerization products.Showed genetic profiling products for the ITS using a pair of initiator ITS3 - ITS4 where showed variation in the ITS region area for the pair in the public initiator profiling C.albicans under study

دراسة تاثير البروبايويك في تكوين الغشاء الحيوي وانتاج البروتيز لبكتريا Pseudomonas aeruginosa المعزولة من اخماج الحروق والجروح == Study The Effect of Probiotic On Biofilm Formation And Production Protease Enzyme By Pseudomonas Aeruginosa Isolated From Contaminated Burns And Wound

اسم المؤلف: علا عبد الكريم كاظم النعيمي
اسم المشرف: منيرة جلوب اسماعيل العبادي | ضيماء محمود ابراهيم
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: he study includes the collection of 70 burns and wound swabs from patients of different hospitals, in Baghdad area, for the period from November 2013 to February 2014. 2) 31 isolates characterized as Pseudomonas aeruginosa 9 (45%) from wound and 22 (78.57%) from burn swabs, depending on the characteristics of the colonies phenotypic and microscopic when grown in differential selective media, as well as biochemical tests todiagnose isolates, Then the identification of these species were confirmed by using the system (GN I Card) using Vitek 2 device complementary step.3) The study includes sensitivity test towards 16 antibiotics for each species by using the system (AST Card) using Vitek 2 device, and the isolates ware Multi drug resistance, As results all isolates were resistant by 100 % for each of Piperacillin, Ticarcillin, Ticarcillinclavoulanic acid, Cefazolin, Ceftriaxone and Tigecycline. All strains were less resistance to Ceftazidime with percentage 50%. 4) All the isolates of Pseudomonas aeruginosa with percentage %100, have ability to produce protease enzyme.5) This study was use tow methods of biofilm formations seen in P. aeruginosa, Micro - titer plate method (MTP) and Tube method (TM), Results revealed that MTP was found to be more sensitiveand specific method for biofilm detection than TM. 6) Well diffusion method revealed the inhibitory effect of bacteria Lactobacillus acidophilus, Lactobacillus rhamnosus GG and its supernatants in the growth of bacteria Staphylococcus aureus and P. aeruginosa, and was the supernatant of L. rhamnosus GG isthe largest inhibitory effect on the growth of S. aureus and P.aeruginosa with inhibition diameter 32 and 34 mm respectively, while the inhibition of growth by supernatant of L. acidophilus diameter 25 and 27 mm respectively. 7) Estimated the amount of protein in each of the biosurfactant and bacteriocine producers of L. rhamnosus GG bacteria concentration of 74, 54 Mg/mL respectively, while their concentration in bacteria L. acidophilus 44, 40 Mg / mL, respectively.8) Found that the bacteriocine product of bacteria L. rhamnosus GG and L. acidophilus GG have inhibitory effect on ability of P. aeruginosa to produce the enzyme protease, and the absence of this effect in the Biosurfactants the product of these two types.9) The biosurfactant product from L. rhamnosus GG showed inhibitory effect greater than the effect of the biosurfactant product from L. acidophilus to inhibition (production of biofilm and adhesion to epithelial cells).10) studied the suspension's effect of L. acidophilus and L. rhamnosus GG in contrived injuries wounds in mice contaminated with P. aeruginosa, Positive results have been shown to be affected by the contaminated bacteria injuries wounds.

دراســــة الفعالية التضادية لجرثومة Bifidobacterium spp تجاه جرثومة Staphylococcus aureus المقاومة للمثيسلين == Studying The Antagonistic Activity of Bacteria Bifidobacterium Spp Against Bacteria Methicillin Resistance Staphylococcus Aureus

اسم المؤلف: انغام نجاح هادي الخفاجي
اسم المشرف: سهام جاسم الكعبي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: تضمنت هذه الدراسة التحري عن الفعالية التضادية لجرثومة Bifidobacterium spp تجاه عزلات سريرية لجرثومة Staphylococcus aureus المقاومة للمثيسلين، ودراسة تاثير تلك المواد على عوامل الضراوة المنتجة من قبل S.aureus المقاومة للمثيسلين مثل الغشاء الحيوي Biofilm وا | This study included the investigation of Bifidobacterium spp antagonistic activity against Staphylococcus aureus isolates, and the effect of these antimicrobial agent on virulence factors produce by MRSA (Methicillin Resistance Staphylococcus aureus) such as Biofilm and Slime layer. One handered different samples were collected to isolate Bifidobacterium spp and 50 clinical isolates of Staphylococcus aureus from clinical specimens in AL - Najaf AL - Asharaf governorate during the period of (10/10/2013 - 20/1/2014) by the cultural characteristic colonies, microscopic for cells, biochemical tests and polymerase chain reaction PCR technique to detect 16SrDNA, the results showed that 13 isolates belong to Bifidobacterium spp and there are divergent gene content between these isolates and they have been bearing lm26/ lm3 gene. Carbohydrate fermentation test was used to distinguish the species of Bifidobacterium, the results revealed that the B.bifidum is more predominant than the other species; B.thermoacidophilum, B.adolescentis, B.breve, B.longum. Antibiotics susceptibility test to Staphylococcus aureus using antibiotic disc diffusion assay showed that Methicillin Resistant Staphylococcus aureus formed 70% and 30% of isolates were sensitive to the same antibiotics, also the ability of MRSA isolates to produce Biofilm and Slime layer was investigated by tube method and Congo red agar, then 10 isolates of Staphylococcus aureus were selected as efficient isolates according to the resistance of Methicillin and the high ability to produce biofilm and Slime layer. The antagonistic activity of Bifidobacterium spp was extracted and tested against these MRSA isolates, the results showed that the most isolates of Bifidobacterium spp were effective against Methicillin Resistant Staphylococcus aureus (MRSA), as well as the inhibition of growth and formation of Slime layer by using the wells and disc diffusion agar assay. The inhibition zone was between (10 - 30)mm, and the ability of bacteriocin production of Bifidobacterium spp against MRSA have been conducted by well diffusion broth method, B.bifidum3 was greater than other species in producing bacteriocin with asignificant increase (P<0.05). Furthermore the impact of bacteriocin production to inhibit the development and formation of biofilm was investigated, the results showed that the bacteriocin has ahigh activity against MRSA. The bacteriocidal and bacteriostatic effect of bacteriocin was conducted by using spectrophotometer, the results showed that these effects were bacteriostatic in the beginning of the growth MRSA while it was lethal during other time and 40 minutes is the period needed for killing MRSA bacteria.

دراسة بكتريولوجية لاخماج المسالك البولية للنساء الحوامل والمصابات بارتفاع ضغط الدم وداء السكري في منطقة تكريت == Bacteriological Study of Urinary Tract Infections Among Pregnant, Hypertensive, And Diabetic Women in Tikrit Region

اسم المؤلف: اسراء عز الدين ابراهيم
اسم المشرف: عبد الكريم فتاح عمر
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: النساء المتزوجات والحوامل والنساء المصابات بداء السكري وضغط الدم المرتفع ومدى مقاومتها للمضادات الحيوية لقد اشتملت الدراسة على 411 عينة ادرار من المريضات المراجعات لمستشفى تكريت التعليمي و100 عينة ادرار كمجموعة ضابطة للفترة من تشرين الثاني2008 ولغاية ايلو | This study aimed to isolate and identify the bacterial causative agents of urinary tract infections among married, pregnant, diabetic and hypertensive women and to determine the susceptibility of isolated bacteria to different antibiotics. The study included 411 urine samples for women attended to Tikrit Teaching Hospital, besides 100 urine samples used as a control group. The study carried out for the period from November 2008 to September 2009.E. coli was the most isolated bacteria at the rate of 31.8 % from married women followed by Staphylococcus saprophyticus 18.18 % and Staphylococcus aureus 15.90%. In the pregnant women, S. saprophyticus was the most isolated bacteria 48.10 % followed by E. coli 20.25 % and S. aureus 12.65 %, and in the diabetic women, S. saprophyticus was the common isolated bacteria 32.07 % followed by E. coli 28.30 % and S. epidermidis 15.09 %. In the hypertensive women, S. saprophyticus 30.35% was the common isolated bacteria followed by E. coli 25.0% and S. aureus 19.64 %. In the case of diabetic pregnant women, and in the hypertensive pregnants S. saprophyticus was the most isolated bacteria, (80 % and 75 % respectively), while E. coli 44.4% was the common isolated bacteria among the diabetic hypertensive women. Out of all isolates, S. saprophyticus 35.13 % came in the first rank followed by E. coli 26.64% and S. aureus 12.74% while Klebsiella oxytoca and Serratia odorifera (0.38% for each of them) were the lowest isolates. Urinary tract infection was common among urban patients 57.49%.The isolated bacteria showed different susceptibility to the antibiotics used in this study. S. saprophyticus isolates showed high sensitivity to Amikacin, Nitrofuratoin and Cefolaxime (the resistance rate for these antibiotics were 11 %, 12.1 % and 12.1 % respectively). While these isolates showed high resistance to Streptomycin 76.9 %, Nalidixic acid 67 % and Cephalexin 69.29 %. E. coli isolates showed low resistance rate to Amikacin 9.28 %, Ciprofloxacin 10.1 %, whereas showed high resistance rate to Cephalexin 47. %, Streptomycin 84 % and Ampicillin 82.6 %. The isolates of S. aureus showed low resistance to Ciprofloxacin 18.2 %, Carbencillin 18.2 % and Amikacin 15.2 % and high resistance for Ampicillin 18.8 %, Nalidixic acid 75.8 % and Cephalexin 72.79 %. Other isolated bacteria showed a different percentages of susceptibility to antibiotics Serratia odorifera, Proteus vulgaris and Enterococcus faecalis showed a significant resistance to antibiotics, while S. epidermidis isolates showed low percentages of resistance to Ciprofloxacin 9.1 %, Amikacin 18.2 % and Gentamicin 9.1 %. For S. saprophyticus the minimal inhibitory concentrations (MIC) resulted from Ciprofloxacin, Gentamicin and Cefotaxime were 8 - 32, 8 - 64 and 4 - 8 ?g/ml, and for E. coli the MIC results were 2, 8 - 16 and 16 - 64 ?g/ml. The MIC results were 2 - 8, 8 - 128, 32 - 256 ?g/ml for S. aureus, while the MIC results were 8 - 32, 16 - 64 and 16 - 128 ?g/ml for Klebsiella pneumoniae.

عزل وتشخيص المبيضات من المرضى المصابين بداء السلاق الفموي في محافظة النجف الاشرف مع دراسة جزيئية لبعض عوامل الضراوة == Isolation And Identification of Candida Spp. From Patients With Oral Thrush In Al - Najaf Province And Molecular Study of Some Virulence Factors

اسم المؤلف: ايهاب يحيى جابرالعلياوي
اسم المشرف: فاطمة عبد الحسين التميمي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: تشمل هذه الدراسة بعض المعايير السريرية لمرضى نقص المناعة (مرض السكري والسرطان وزرع الكلى)، والمصابين بداء المبيضات الفموي والتي تضمنت العمر والجنس. الهدف من هذه الدراسة هو عزل وتشخيص انواع المبيضات. بطرق مختلفة بما في ذلك التشخيص المباشر، والتحضين في الم | The present study was conducted to isolation and identification of Candida spp. Isolated from immunocompromised patients with (Diabetes, Cancer & Kidney transplant) by different identification methods including direct examination, laboratory culture, biochemical tests and molecular method using polymerase chain reaction (PCR) technique and determine the virulence factors phenotypic to Candida spp. and genetic polymorphism in Candida albicans in secreted aspartyl proteinase (SAP2). During the period from October 2013 to February 2014, a total of 140 oral swab were collected from immunocompromised patients with attending to the three center in AL - Sadder Medical City (Oncology center and kidney center and Center for Diabetes and Endocrinology).In AL - Najaf Governorate, the samples were collected as following : - 110 mouth swabs from patients suffering from leukemia, prostate, stomach and bowel cancer, also 10 mouth swabs from kidney transplant patients and 20 mouth swabs from diabetes. The percentage of female to male was as following, female (86) 64(72.7%) infected, 22(42.3%) uninfected and male (54) 30(57.7%) uninfected, 24(27.3%) infected, with age range between (5 - 64) years. In this study, the results show that the incidence of candidiasis in women was higher than the male also the most cases of candidiasis in patients age range between (45 - 54) years. The results of the present study reveal that the prevalence of oral candidiasis from patient with cancer was more than diabetes & kidney transplant patients which was 72(81.18%), 12(13.65%) & 4(4.54%) respectively. When culturing oral samples on the primary isolation media Sabouroud dextrose agar (SDA) and the secondary media on CHROM agar, The results reveal the presence of 88 isolates belong to Candida spp.which include : - 42 (47.68%) belong to Candida albicans, 20 (22.72%) belong to Candida. dubliniensis, 18 (20.5%)belong to Candida krusei, 6 (6.8%) belong to Candida glabrata & 2 (2.3%) belong to Candida parapsilosis. According to this result, Candida albicans was found the most frequently isolated species and the occurrence of the other non - albicans isolates such as Candida dubliniensis and which followed Candida albicans in frequency of the isolates in oral candidiasis. In this study, the ability of C.albicans & non - albicans was tested to produce some virulence factors such as production of enzymes phospholipase, lipase, germ tube, chlamydospore & biofilms when culturing Candida spp. on different media. The results of the culture show that Candida albicans produces all virulence factor included germ tube, chlamydospore, biofilms & phospholipase. Some species share several phenotypic characteristics ability to produce enzymes of phospholipase such as C.krusei while C.dubliniensis produce chlamydospore, when culturing in the Casein agar & produce biofilms, In addition the molecular tests to was used for Candida albicans confirm the ability to secreted aspartyl proteinase (ASP2). This study showed the amplified from the Candida spp. as following : - C.albicans 306 bp, C.glabrata 594 bp, C.parapsliosis 387 bp, C.krusei 651 bp & C.dubliniensis 451 bp.

دراسة بعض الجوانب المناعية والبكتريولوجية لمرضى ذات الرئة Pneumonia == Study Some Immunological And Bacteriological Aspects of Pneumonia Patients

اسم المؤلف: كرم رياض حسن الجراح
اسم المشرف: رسمية عبد ابو ريشة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: جمعت 120 عينة قشع من اشخاص مصابين بمرض ذات الرئه للمدة من ايار 2013 الى شباط 2014 من ثلاثة مستشفيات في مدينة بغداد وشملت مستشفى اليرموك التعليمي, مستشفى دار التمريض الخاص, مستشفى بغداد التعليمي. شخصت العينات باستخدام الفحوص البايوكيميائية, نظامApi 20 E و| From May 2013 to Feburey 2014, 120 sputum and Aspiration samples of patients with pneumonia disease were collected from different hospitals in Baghdad included : Al Yarmouk Teaching Hospital, Hospital Nursing Home sector and Baghdad Teaching Hospital. All samples were diagnosed by biochemical tests, Api 20 E and Api20 strep. Systems, The results was found to be 28 isolates (23.3%) belong to K.pneumoniae, 26 isolates (21.7 %) belong to S.pneumonia while 66 isolates(55%) belong to causes : E.coli, Pseudomonas sp., Moraxella catrrhalis, S.pyogens, Monilia and S.aureus. From (55) blood samples of pneumonia patients and 30 blood samples from healthy individuals, number of white blood cell (Neutrophil) counts were measured, percentage of Neutrophil cell count in female (53%) higher than the cell count in male(47%). Some markers of pneumonia disease were studied such as ratio of infection between male and female, chronic and acute cases and smoker and non smoker, the results were showed the ratio of infection in female more than in male, (60%, 40%), respectively. And number of acute cases of pneumonia patients 41(74.55%) more than chronic cases 14(25.45%). While infection ratio in smoker patients more than nonsmoker patients at ratio 24(57.14%)18(42.86%), respectively. The result of interleukin - 8 level through acute and chronic phases in pneumonia patients sera was showed high level in patients compared with healthy persons (86.4403 ± 25.50919 vs. 58.7636 ± 4.73152 pg / ml, respectively) with a non significant difference statistically (P?0.05). Also in the age group (age 15 - 60years), The results showed the results interleukin 8 levels higher than the age groups of (2month - 3years) and (age 60 - 85) (93.63 ± 51.65, 68.88 ± 17.17, 65.68 ± 11.73 pg / ml, respectively) with non significant difference (P?0.05). The result of Leukotriene B4 level through acute and chronic phases in pneumonia patients sera showed high level in patients compared with healthy persons (36.00 ± 3, 82 vs. 25.96 ± 4.44 pg / ml, respectively) with a significant difference (P?0.05). Also in the age group (two months - 3 years) were showed the results interleukin 8 levels higher than the age groups (age 15 - 60) (age (60 - 85)(33.61 ± 11.06, 19.29 ± 4.67, 16.86 ± 4.51 pg / ml respectively) with no siginificant difference (P?0.05), the correlation coeifficient between interleukin - 8 and leukotriene B4 was (0.12) with no siginificant difference. The phagocytic activity was determined in pneumonia patients sera according to measurement C3 protein level through acute and chronic phases, the result were showed the ratio of C3 protein levels in healthy persons more than in patients (135.93 ± 12.74, 133.19 ± 12.01 pg / ml, respectively) with a difference was not significant statistically (P?0.05).

عزل وتشخيص بعض فطريات الاذن الخارجيه واختبار قدرة الفطر Aspergillusعلى افراز بعض انزيمات عوامل الضراوة == Isolation And Identification of Some Fungi of Otitis Externa And Testing The Ability of Aspergillus Spp. To Produce Some of Virulence Factors Enzymes

اسم المؤلف: وفاق احمد محمود
اسم المشرف: سميرة كاظم حميد | ياسر لفتة حسون
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: جمعت (113) عينه سريريه (مسحة اذن Ear swab) لمرضى التهاب قناة الاذن الخارجيه الفطري Otomycosis من كلا الجنسين والذين راجعوا استشارية الانف والاذن والحنجره في مستشفى الصدر التعليمي والعيادات الخاصه للاطباء الاختصاص / محافظة النجف الاشرف للمده من تشرين الثان | This study included 113 ear swabs from patients with otomycosis, males and females, visited the otolaryngology consultation room at Al - Sader Medical City in An - Najaf / Iraq in addition to the private otolaryngology clinics of some of ear, nose and throat specialists, during the period from October 2012 to March 2013, the study aimed to isolate and diagnose the causative opportunistic mycotic infections of the ear and to test sensitivity of these organisms towards antimycotic agents in addition to identifying some enzymes of the most fungal reluctant type phenotypically and molecularly. (103) positive swabs were obtained from total samples including "Cephalosporium, Candida spp, Aspergillus spp, Neurospora and Trichosporium beigelii.The Precentage of isolated fungi was (85.43)% for Aspergillus, (10.67)% for Candida, while other species constituted totally (3.88)%. The study show that the highest percentage of isolated fungi was from males as it was(51.4)% while in females was (48.6)%. Patients' ages were found to be between (11 - 80) years and the age group (21 - 30) years was the most frequent Category in (30.3)% and the Category (71 - 80) years was affected only in (0.8)%. The highest percentage of insulation fungi during the period of sample collection was in February which constitute (30.9)% and the lowest percentage was in March reaching only (14.1)%.The highest ratios to isolate the fungus was recorded among housewives and unemployed males and it was (44.2%), while the lowest rates of insulation when the students reached (15.9%).The cases of otitis media excelled on the rest of the pathological cases accompaniments of Otomycosis as the proportion of insulation was (19.4%), followed by disease of blood pressure which their percentage was (5.3%), while the rest of the pathological cases did not have significant influence in insulation, for more ear side affected the left ear was affected in(51.8)% and the right was affected in (48.2) %. The study showed that there is inhibitory effect of (flucanazole, Nystatin, Clotrimazole, Miconazole, Itraconazole and Ketoconozole) against Aspergillus spp (A.niger, A.flavus and A.terreus). All antifungals were effective and their inhibitory ratio was (100) %, except for the antifungal (Fluconazole ) which found to have a lower inhibitory effect (10) % against the species A.flavus only. Testing the phospholipase activity for aspergillus species, A.flavus and A.niger showed phospholipase activity in two degrees (++, +++), but A.terreus shoed only (++). Searching the gene alp " which is encoded for the production of alkaline protease " showed that all the Aspegillus species produces the gene with amplification results of (100)% for the band supposed to appear at the molecular weight of (747) pb

التمنيع باضداد الخمل النوع الاول المنتجة من بكتريا الاشريكية القولونية المسببة لالتهاب المجاري البولية ضد استيطان واصابة المثانة == Immunization With Type 1 Fimbriae of Uropathogenic Escherichia Coli Against Colonization And Infection of Bladder

اسم المؤلف: اروى علي شكر
اسم المشرف: رسمية عبد ابو ريشة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Between September and November 2013 a total of 140 urine specimens obtained from patients in Al - Kadhmiah Teaching Hospital, Ibn - Al Naffees Teaching Hospital, and Educational Laboratories in Medical city. These midstream urine specimens were obtained from patients clinically suspected to have urinary tract infection (UTI) in sterilized containers. All specimens were processed immediately after collection.A total number of 140 specimens of mid - stream urine were collected from patients suffering urinary tract infection symptoms. These isolates were obtained from 41 male and 99 female patients. They were distributed in the age group of 5 - 60 years old. All specimens were identified using biochemical tests and API 20 E system. 60 (42.85%) of urine specimens was Escherichia coli and 15(10.71%) of specimens growth was Klebsiella spp. and 25(17.85%) growth was Proteus spp. and 40 (28.57%) was G+ve bacteria.The adherence ability of E.coli isolates were evaluated by using Congo red agar and detected their ability to produce biofilm by using methylene blue staining technique in polystyrene microtiter plates and then Optical density was determined at 580 nm.All sixty isolates of E.coli were grown on Congo red agar medium to detect their adherence ability. Twenty (33.3%) isolates were given negative result by forming pink colonies on congo red agar, were as, forty (66.6%) isolates were given positive result by forming black colonies with a dry crystalline. Three isolates give strong results E.coli (13, 8, and 40). Consistency indicated biofilm production by microtiter plate. E. coli 40 which isolated produced the thickest biofilm (O.D. : =3.17).Type 1 fimbriae expression by E.coli 40 isolate was detected by mannose - resistant hemagglutination (MRHA) of human blood group (O) IRBCs in the presence of 2% D - mannose. The isolate showed strong MRHA characteristics of type 1 fimbriae under microscope (40x). The E.coli E.40isolate which produced the thickest biofilm and gave strong MRHA was elected to be the source of type 1 fimbriae.Type 1 fimbriae was extracted by heating and mechanical shearing and partially purified by Ultracentrifugation (48, 000xg for 3hrs). SDS - PAGE with a molecular weight 20, 000 Dalton.Anti - type1 fimbriae antisera were prepared in rabbits. The specificity and titration of anti - type1fimbriae antibodies were determined using IgG protein by radial immunodiffusion plate and bacterial agglutination. IgG antibodies to fimbriae type 1 were also detected in rabbit sera from immunized rabbit and non - immunized. Immunized rabbit test 1 had increases in Fim1 - specific IgG antibodies (9.5) mg/dl. Immunized rabbit test 2 had increases in Fim1 - specific IgG antibodies (8.6) mg/dl. The non - immunized rabbit had no increase in absolute IgG antibodies to Fim1.The antisera agglutinated bacterial cells up to 1/80 dilution confirming the presence and titer of specific antibodies against type1 fimbriae.The microtiter plate method was applied to estimate the role of the prepared anti - type1fimbriae antibodies in reducing the biofilim formation by E.coli. Prevention of bacterial adherence and subsequent biofilm formation to polystyrene microtiter plate was studied by employing different dilutions (1/10, 1/20, 1/40, 1/80, 1/160, 1/320, 1/640) of rabbit sera containing anti - type 1 fimbriae antibodies. The maximum inhibition of biofilm formation in terms of optical density (580 nm) was found in lowest dilution (1/10) (highest concentration of Abs). However, the minimum inhibition was observed in highest dilution of rabbit antisera (1/640) (lowest concentration of Abs). Here the inhibition occurs in a dose dependent manner as the biofilm formation increased dramatically with increasing in antisera dilution. The results also showed that there is a significant difference(P<0.05) among data treated with sera and data without sera and among serial dilutions.General urine examination and culture for urine that absorbed from test 2 and control was done (GUE) show that pus cell in urine was (1 - 2/HPF). There was no growth of bacteria in urine culture.The Histological section show that the Control and test 1 rabbit (have been immunized with fimbriae and adjuvant and injected with E.coli intra muscular) and Test 2rabbit (that has been immunized with fimbriae and adjuvant and injected with E.coli directly in bladder has normal epithelial cells and mucosa.

دراسة وبائية لاميبا النسج في مدينة كركوك وعلاقته بالجذور الحرة في مصل دم المصابين == Epidemiological Study on Entamoeba Histolytica in Kirkuk City And Its Relationship With Free Radicals in Blood Serum of Infected Persons

اسم المؤلف: ليما طارق يواش لازار
اسم المشرف: شهاب احمد محمد الجبوري | محمد عبد العزيز قادر
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية فحص 600 عينة غائط للمرضى المراجعين لمستشفى الاطفال في مدينة كركوك للفترة من 1/12/2010 الى 17/7/2011 للتحري عن طفيلي Entamoeba histolytica.تم فحص عينات الغائط بطريقة المسحة المباشر باستخدام المجهر الضوئي للكشف عن الاطوار المتغذية وال | The present study includes the examination of stool sample of patients attended pediatric hospital in Kirkuk city for period from 1st of December till 17th July 2011 for detection of Entamoeba histolytica.The stool samples were examined by direct wet mount method, using light microscope for detection of trophozoites and cysts stages of parasite. The study showed that the rate of infection with E. histolytica / E. dispar was 21.67% (130 of 600). The cystic stages were dominant in infected stool samples which being 53.85% (70 of 130), while the trophozoite stage was 15.38 % (20 of 130). The epidemiological survey based on direct stool examination showed the rate of infection among females was 23.07% (60 of 260) in comparison with males 20.59% (70 of 340). The highest rate of infection was among the age group 1 - 5 years 25.5% (69 of 270). The highest rate was also in children of illiterate parents 26.1% (68 of 260). The biochemical tests were carried out to estimate serum cholesterol, triglycerides, GOT, GPT, malondialdehyde (MDA) and selenium. The study showed that the level of serum cholesterol, triglycerides, GOT, GPT, malondialdehyde increased in infected patients in comparison with healthy controls, while the study showed decreased selenium concentration in infected patients in comparison with healthy controls. The enzyme linked immunosorbent assay(ELISA) was done to estimate serum IgG level for Entamoeba histolytica, the rate of seropositivity for IgG was 21.68% (18 of 83). The rate of IgG in positive sample was 17.73% (7 of 41) while in negative sample was 26.19% (11 of 42).

دراسة وبائية على طفيلي المشعرات المهبلية Trichomonas vaginalis وبعض الجراثيم المسببة للامراض المنقولة جنسيا وتاثير بعض المستخلصات النباتية على نمو الطفيلي في الزجاج في مدينة كركوك == Epidemiological Study on Trichomonas Vaginalis & Some Associated Bacteria That Causing Sexual Transmitted Diseases & Effect of Some Herbal Extraction on The Parasite in Vitro in Kirkuk City

اسم المؤلف: محسن عز الدين سليمان
اسم المشرف: ابراهيم شعبان داوود | محمد عبد العزيز قادر
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: بلغ العدد الكلي للعينات التي جمعت وفحصت للفترة من بداية شهر تموز 2007 الى نهاية شهر مايس 2008 (2345) عينة لكلا الجنسين مــن مستشفيات كركوك للتحري عن وبائية طفيلي المشــــــعرات المهبليـــــــــة Trichomonas vaginalis وبعض الجراثيم المسببة للامراض المنقو | A total (2345) specimens were collected & examined from both sexes, from the beginning of July 2007 till the end of May 2008 attending Kirkuk hospitals for epidemiological study of Trichomonas vaginalis & some sexual transmitted microorganisms. The vaginal examinations were done by gynecologist, two high vaginal swabs were taken from (250) women & a cervical swab from some women with vaginal discharge. General urine examination & blood group with Rh factor were performed for each woman with vaginal discharge. Direct microscopic examination, staining & culture of swabs were performed on different methods for diagnosis of Trichomonas vaginalis, followed to diagnose Candida albicans, Neisseria gonorrhoeae, Chlamydia trachomatis & Treponema pallidum as well as some other bacterial groups. The rate of Trichomonas vaginalis was (2.8%) & Neisseria gonorrhoeae (0.8%), while no Chlamydia trachomatis & Treponema pallidum recorded in this study. The highest rate of Trichomonas vaginalis was among the age group between 15 - 29 years (3.6%), the rate of Trichomonas vaginalis infection was higher among illiterate (5.7%) than educated ones. The highest rate of Trichomonas vaginalis was recorded among those with blood group O & Rh (+ve) (3.8%). The same result was found regarding other microorganisms in both sexes. Statistically the results were not significant.The rate of infections in females was higher than males in all infection except Neisseria gonorrhoeae was recorded highest rate in males(35%). The percentage of mixed infections with Trichomonas vaginalis was Candida albicans (1.6%) & B - hemolytic streptococcus (0.4%). The maximum infections rate in vaginal swabs were Staphylococcus aureus (42.3%) & lowest rate of Neisseria gonorrhoeae (2%). Regarding the distribution of microorganisms isolated from urine samples of females suffering from vaginal discharges, the highest rate of infection was Eschirechia coli (22.2%) & lowest rate of Neisseria gonorrhoeae (2%) respectively. The rate of isolated microorganisms from urethral discharge of males were Neisseria gonorrhoeae (35%) & Escherichia coli (5%). Antibiotics Ciprofloxacin & Amikacin showed good inhibition activity against all pathogenic microorganisms. Regarding the effect of different concentration of metronidazole & medical herbs to the Trichomonas vaginalis invitro, metronidazole showed clear effective inhibition at concentration (1.25%) during 24 hours. The inhibitory effect of Zea mays (both alcoholic & watery extractions) was greater than Apium graveolens & Foeniculum vulgare. At concentration (2.5% & 5%) of Zea mays in both extractions was almost identical to metronidazole. Six solvents were used by thin layer chromatography (TLC) in order to separate alcoholic & watery extraction of medical herbs, only three of them A, B & F gave good results in separating herbal extraction with differing in its component. By measuring relative flow rate (Rƒ) of all compounds that separated and determind. The chemical compounds of each herbal extract were established & included alkaloids, flavonieds, amines, pigments, oils, carbohydrates, phenols, purines & tanines.

عزل وتشخيص بعض انواع الجراثيم المسببة لالتهابات الزائدة الدودية في مدينة تكريت == Isolation And Identification of Some Baceterial Speices Which Caused Appendices Infections in Tikrit City

اسم المؤلف: عبد عبد الله محمد الجبوري
اسم المشرف: اميرة محمود محمد الراوي | علي صالح حسين الجبوري
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: تضمنت الدراسة جمع 75 عينة من الزائدة الدودية (Appendices) مباشرة بعد استئصالها من المرضى الراقدين في مستشفى تكريت التعليمي ومن كلا الجنسين، الذين خضعوا لعملية استئصالها وللمدة من اب عام 2008 ولغاية نيسان 2009. عزلت الجراثيم من العينات باستخدام الاوساط الز

تحديد مقاومة بعض الجراثيم المعزولة من اخماج في مستشفى تكريت التعليمي للمضادات الحيوية وعلاقتها بالبلازميدات == Determination The Antibiotic Resistance of The Some Isolated Bacteria From Tikrit Teaching Hospital Infections And Their Relationship With Plasmids

اسم المؤلف: شيماء عبد محمد علي
اسم المشرف: وعد محمود رؤوف | خالد عمر محمد علي
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: جمعت (140) عينة من المرضى الراقدين لثلاثة ايام فاكثر في مستشفى تكريت التعليمي للمدة من 1 - 8 - 2009 ولغاية 1 - 12 - 2009 بهدف تحديد العزلات الجرثومية الاكثر شيوعا ضمن اخماج مستشفى تكريت التعليمي ومقاومة هذه العزلات للمضادات الحيوية ودراسة العلاقة بين مقاو | One hundred fourti samples were collected from hospitalized patients for three days or more in the Tikrit Teaching Hospital during the period from 1/8/2009 until 1/12/2009 to determine the most commonly isolated bacterial from nosocomial infections in Tikrit Teaching Hospital and their resistance to antibiotics and to study the relationship between antibiotics resistance and plasmid content in the most commonly isolated bacteria and to evaluate the prevalence of these plasmids in the hospital environment. These samples include : (75) Urine, (28) stool, (28) of wound swabs and (9) sputum samples.The incidence of nosocomial infection was (45.7%) of them, urinary tract infections were (50.7%) from (75) samples, followed by wound infections (42.9%) from (28) samples, gastrointestinal infections (39.3%) from (28) samples then pneumonia (33.3%) from (9) samples the nosocomial infections were more common in females and age group 51 - 89 years. Different bacterial species were isolated in this study and the results showed that the most commonly isolated type was Citrobacter koseri in a proporation (21%) of urinary tract infections, followed by the following bacterial species : Escherichia coli (15.7%), Staphylococcus schleiferi (10.5%) , Staphylococcus epidermidis (7, 8%), Proteus vulgaris, Staphylococcus saprophytica, Klebsiella ozaenae, Klebsiella ornithindytica in aproportion (5.2%) for each, Enterobacter aerogenes, Providencia stuartii, Staphylococcus warneri, Staphylococcus aureus, Proteus mirabilis in aproportion (2.6%) for each.Citro. koseri were also the most common in wound infections (25%), followed by the following bacterial species : E.coli (16.6%), P.mirabilis (16.6%), Staph.schleiferi, P.vulgaris, Prov.stuartii and Prov.rettgeri in aproportion (8.3%) for each. Citro.koseri were also the most common in the gastrointestinal tract infections in a proporation (36.3%), followed by E.coli (18.1%), S.flexneri (18.1%), P.mirabilisc, Prov.stuartii, Prov.alcalifaciens in a proporation (9%) for each. . While Strept.pneumoniae was the most common(66.7%) in penemonia cases followed by K.pneumoniae (33.3%).The isolated bacteria were tested for antibiotics susceptibility and it has been found that most of these isolates showed high resistance to the studied antibiotics other than Prov. Stuartti was sensitive to all antibiotics except of Rifampicin and Tetracycline. As well as bacterial species belonging to the genus Staphylococcus were sensitive to Amikacin, Staph.aureus was also sensitive to Nitrofurantoin while Staph.warneri was also sensitive to Ciprofloxacin and Nitrofurantoin also Enterobacter aerogenes was resistant to all antibiotics except for Ciprofloxacin and Nitrofuratoin. Eleven of most commonly isolated bacteria which were multidrug resistant were chosen for the curing experiments using SDS to study the relationship of their plasmids with resistant to antibiotics. The results showed that the cured cells of E.coli lost their resistance to Tetracycline Ciprofloxacin and Cefotaxime which Citro.koseri lost their resistance to Tetracycline while K. ornithinolytica lost resistance to both antibiotics Tetracycline and Ciprofloxacin either P.mirabilis lost resistance only to tetracyclin as well as Strept.pneumoniae lost their resistance to Cefotaxime, tracycline and Ampicillin. Which may mean that the genes responsible for resistance to these antibiotics are located on plasmids as proven by the results of electrophoresis.Electrophoresis results also showed that all studied bacterial isolates had the same plasmid profile which may regarded as an epidemiological pointer that plasmids are conjugative which have the ability to transfer antibiotic resistance to other bacterial strains of the same species or to other different bacterial species.

التحديد الجزيئي لبعض عوامل الضراوة في بكتريا Klebsiella spp المعزولة من مصادر سريرية مختلفة == Molecular Identification of Some Virulence Factors In The Bacterium Klebsiella Spp Isolated From Different Clinical Sources

اسم المؤلف: فرقد حسن فليح
اسم المشرف: بهيجة عبيس حمود الخالدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: جمعت (150) عينة من المرضى المراجعين والراقدين في مستشفى الديوانية التعليمي والذين يعانون من اصابات مختلفة وللفترة من 1/كانون الاول/2013 ولغاية 1/نيسان /2014 وتضمنت هذه العينات ماياتي : (50) عينة حروق وجروح، (50) عينة ادرار و(50)عينة قشع من المرضى المراجعين | One hundred fifty samples were collected from patients admitted in Al - Diwaniyah Teaching Hospital and suffering from various injuries and for the period from 1 / December / 2013 and up to 1 / April / 2014 these samples in clude : (50) sample burns and wounds, (50) urine and (50) sputum.The results showed that 90 samples (60%) of the total number gave is positive result for the growth.the bacterial isolates were distributed among 35 samples (70%) of burns and wounds, (33) the sample (66%) from urine and (22) sample (44%) from sputum After biochemical and cultural test found that 99 isolate belonging to Different Genus and species of bacteria these isolates include 40 isolate (44.44%) bound and wound isolates Staphylococcus sp was the predoniant in these samples by rate (42.5%), 36 isolate(40%) E.coli was most isolate by rate 50% in urine sample and 23 (25.5%) Klebsiella pneumonia was the most common isolate by rate (78.26%) in these putum.. Sensitivity test results of antibiotics showed that most of the isolates have high resistance against antibiotics used in the study and the most isolates possess multiple resistance (Cross resistance) as shown resistance to most of antibiotics that used in these study. All asolates (% 100) were resistant to nitrofurantin and Tetracycline while(95%)is resistant to ampiciline (90%) of isolate were resistant to thired generation of cephalosporins (ciprofloxacin and ceftazidime) and (%80) of isolate wereresistant to gentamicin and impeneme, finely (%80)of isolate were resistant to azetroname..The results of the molecular detection of genes uge and mag A gene in Klebsiella sp in the 16 (50%) of the isolates had mag A as the 8 (44.44%) of the isolates bacteria K.pneumonia carrier of mag A and 8 isolates of type K.oxytoca had of the gene above the gene uge was his appearance in the proportion of isolates (46.87%) as it appeared in 9 (50%) of the isolates K.pneumonia and 6 isolates (42.87%) of the isolates k.oxytoca..There were relationship between resistance of bacteria to antibiotics and possession of these genes

انتشار المقاومة للمضادات الحيوية وبعض عوامل الضراوة في بكتريا Pseudomonas aeruginosa المعزولة من مستشفيات في النجف المعزولة من مستشفيات في النجف == Molecular Study To Some of Pseudomonas Aeruginosa Virulence Factors Isolated From Clinical And Environmental Isolates

اسم المؤلف: ذو الفقار حيدر حسن
اسم المشرف: ماجدة مالك متعب
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية لغرض التحري عن وجود الجينات الكروموسومية المسؤولة عن بعض عوامل الضراوة المهمة لجنس Pseudomonas aeruginosa كانتاجها للسم الخارجي A والالجنيت اضافة الى الجينات الكروموسومية المسببة لمقاومة الكوينولونات في العزلات السريرية والبيئية لبكت | This study include investigate the presence of chromosomal quinolone resistance genes and virulence genes(toxA and algD) in clinical and environmental isolates from Al - Sadr Medical city.A total of 110 samples were collected from different clinical sources(burns, wounds, and urine), and 50 from the hospital environment.the samples were collected during 10, Oct, 2012 to 2, Feb, 2013. A total number of Pseudomonas aeruginosa isolates accounted for 89 of 160 samples.The distribution of these isolates were 28 from Burns, 14 wounds, 15 urine, and 32 from hospital environments. Identification of isolates has been accomplished depending on phenotypic diagnostic methods and PCR technique.our study proved that PCR technique depending on oprI and oprL genes was a rapid method for identification of non - pigmented isolates. A total of 20 antibiotics resistant isolates were chosen for Molecular studies. The result reveling that 100% of isolates had a chromosomal mutation in the QRDRs of genes (gyrA, gyrB, parC, and parE). High percentage of isolates(45%) carried mutated genes gyrA and parC, followed by 15% of isolates were carried either mutated gene gyrA alone or three mutated genes gyrA, parC, and parE and and 10%of isolates were carried three muted genes gyrA, gyrB, and parC, while gene that encoding to parC mutation were detected in 5% of isolates. Our study is the first study about detection of Quinolone Resistance Determining Regions in P.aeruginosa in Iraq. The results showed the the presence of mexAB - oprM multidrugs efflux system expression in 100%(20) isolates. while the phenotypic test depending on MIC values for ciprofloxacin alone in the presence of efflux inhibitor showed that only 15%(3) isolates had the active efflux pump.

التعبير الجيني للمورث Erg11 وعلاقته بالمقاومة وظاهرة رجوع النمو لبعض عزلات خميرة المبيضات البيضاء المختبرة تجاه مضادات الازول الفطرية == Gene Expression of Erg11 And It’s Relation With Resistance And Trailing Growth Phenomena of Some Isolates of Candida Albicans Tested Against Azole Antifungals

اسم المؤلف: حيدر عبد الحسين عباس
اسم المشرف: عدنان حمد عبيد الحمداني
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: هدفت الدراسة الحالية الى الكشف عن دور المورث Erg11 والتعبير الجيني النسبي له في مقاومة خميرة المبيضات البيضاء C.albicans وعلاقته بنشوء عزلات مقاومة او ظاهرة رجوع النمو Trailing growth لهذه الخميرة عند اختبار حساسيتها الدوائية تجاه بعض مضادات الازول (الفلو | The current study aimed to detect the role of Erg11 gene and its relative expression in the resistance of Candida albicans, and its relationship with the emergence of resistace or Trailing growth isolates when tested its susceptibility against azoles agenst (fluconazole, ketoconazole). A total of 120 specimens were collected from patients of both gender with different ages whom infected with oral thrush, uro - genital and Gastrointestinal candidiasis and admitted the General Teaching Hospital and materinaty and children Teaching Hospital in AL - Diwaniyah city during the period from December/2012 to February / 2013. The isolation and identification of yeast isolates were done based on their morphological, Cultural and biochemical tests in addition to use the confirmatory systems such as the growth on Chrom Agar candida, Api Candida and Tobacco Agar medium to distinguish C.albicans from other yeast spp. Also, some virulence factors of C.albicans such germ tube formation, chlamydospores production, growth at of 45 c and resistance to cycloheximide were detected. The antibiotics sensitivity test was used in two method (disk diffusion & dilution) to determine the sensitive, trailing growth, resistance isolates and minimal inhibitory concentration (MICs) toward azole agents. The qRT - PCR was used to detect the relative quantitative of Erg11 gene expression and its relationship with resistance to azole and emergence of trailing growth phenomena in the presence of Fluconazoles and ketoconazole. The isolation and identification results revealed that the isolation percent of C.albicans was 47.05 % incomparison with other Candida species that were 15.3%, 11.76 %, 8.23 % and 5.9 % for C.krusi, C.dubliniensis, C.tropicalis, C. parapsilasis and C.glabrata , respectively. The antibiotic susceptibity tests of C.albicans showed the presence of resistance precent (38.89%) to fluconazole and the most of sensitive isolates revealed a trailing growth phenomena in the zone of growth inhibition of floconazole in a ratio 55.56%. While the resistance percent to ketoconazole was 27.78 % and the sensitive isolates showed the trailing growth in a ratio (38.89%). The statistical analysis showed a significant differences (P <0.05) among tested treatments. The MICs values of fluconazole against C.albicans isolates were 8, 16, and 64 Mg/ml for sensitive isolates and were 0.125 and 0.5 Mg /ml for resistance isolates and the trailing growth phenomena occurred at MICs value ? 8 Mg/ml within 24 hours of incubation while the values became ? 64 Mg/ml after 48 hours of incubation.There was a significant differences (p< 0.05) among tested treatments isolates incubated at 24 hours while there was no significant differences (P>0.05) at 48 hours of incubation. In respect with Ketoconazole, the resistance of C.albicans was increased after 48 hours of incubation at MICs values 0.125, 0.25, 0.5 and 1 Mg /ml while its was sensitive at values 4, 8 and 16 Mg /ml and the trailing growth phenomena occurred when the MICs ? 2 Mg/ml after 24 hrs of incubation, while it reached to ? 8 Mg/ml after 48 hrs of incubation. There was a significant differences (p< 0.05) among tested treatments incubated after 24 hours while there was no significant differences (P >0.05) among treatments after incubation 48 hours. The results of relative quantitative gene expression for both genes revealed a significant differences (p< 0.05) in gene expression levels through the occurrence of increased in the transcription of mRNA quantity among tested treatments.The up - regulation of Erg11 and fold change in mRNA transcription was 2.498 fold compared with 1.0 for control treatment after 24 hrs of incubation in the presence of antifungal, while the increasment was 12.606 fold after 48 hrs of incubation of isolates with antifungals.

التمنيع والتحدي لبكتريا Citrobacter freundii فاقدة الجدار المسببة لخمج السبيل البولي == Immunization And Challenge For Walled Citrobacter Freundii And Walled Defective That Causing Urinary Tract Infection

اسم المؤلف: عبد الرسول عويد عداي الشبيب
اسم المشرف: قاسم نجم ثويني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: Cryptic bacterial infection has been studied in urinary system to Human, where 50 underwent urine samples from patient mostly based consulting auditors Hilla public Education Hospital for the period from November 2012 until Feb. 2013. Patients ranged in age from 20 - 50 years males and females bacterial infection rate was 70% distributed between the cell wall defective bacteria had 38% and 32% cell - wall bacteria and the results showed 30% as negative cases. Amicroscopic examination of the urine sludge Showed clear sovereignty of non - specialized immune response type of mixed cell 34% followed by mononuclear cell 30%. Samples were cultured on routine media (Blood agar , Macchonky agar) and Modified media (Variant agar, Variant broth). Out put diagnosed by bacterial system diagnostics, phen - otypic, microscopically and biochemistry by using the special test for microorganism diagnosis and using API 20 E and the use VITEK 2 to confirm some samples results. Characterized by colonies of cell wall defective bacteria grow on variant agar it resembles fried egg which could seen after staining Deinse stain. Citrobacter freundii recorded 12%, the highest rate among cellwall defective isolates. Sensitivity test for bacterial isolates were screened from U.T.I both two type (CWB & CWDB) showed the highest rate of sensitive to Impinem 100% represented CWDB, as well as the highest rate of resistance to Ampicillin 100% represented by the same group of isolates. experimental study with urinary tract infection by injecting bacterial stuck of Citrobacter freundii to the three groups of rabbits : first group ( 3 Rabbits ) : immunized with viable surface antigen extracted from CWDB, second group : ( 3 Rabbits ) immunized with heat killed surface antigen extracted from CWB, the third group (4 Rabbits) represented control group it been treated with sterile normal saline. the injection process through urethra, and then underwent a histological study, was histological damage to the members of the animal - derived non - immunized (control group) seemed clear was the appearance of symptoms of the total cellular infiltration and disruption of the mucous layer and epithelial and other symptoms. The immunized animals have shown their ability to defy injury. It is the study of serological reactions to bacterial antigens Citrobacter freundii immunized with vaccines for rabbits given high caliber agglutination with its specialized vaccines and low with adversity and that the absorption and the corresponding and variant antigen does not remove the agglutination effectiveness with immune sera prepared suggesting that the absorption is incomplete and that the difference between the wall and a wall defective in terms of antigenic is quantitative and qualitative.

تاثير انزيم SNase المنقى من العنقودي في الغشاء الحياتي للايشيركية القولونية والكليبسيلا الرئوية == Impact of Snase Purified From Staphylococcus Aureus On Biofilm of Klebsiella Pneumoniae And Escherichia Coli

اسم المؤلف: هند تحسين ابراهيم
اسم المشرف: حارث جبار فهد المذخوري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: SDS - PAGE showed that a single sharp band with an approximately 16.8 KDa molecular weight has been seen. A matter indicates that the enzyme is consistently pure. PCR technique was applied to approve the existence of nuc gene in S.aureus Nevertheless, only those which depicted positive results on DNase agar harboured nuc gene, as it is specified by single band appearance of nuc at assumed molecular weight (ca. 255 pb) of marker that being used. The current study findings highlighted the participation of SNase purified from S. aureus in significant (P< 0.05) preventing biofilm formation by E. coli and K. pneumoniae compared with untreated controls. Evidently, the inhibitory effect of SNase on biofilm formation is undoubtedly perceived, indicating the degradation of the structural major component of biofilm formation (i.e. extracellular DNA). Results revealed that SNase was able to significantly (P< 0.05) reduce the number of the uropathogens; E. coli and K. pneumoniae attached to the uroepithelial cells. 1 Chapter one : Introduction and literature Review 1. Introduction and Literature Review 1.1. Introduction Staphylococcus aureus is a persistent human pathogen that is responsible for a range of diseases that vary widely in clinical presentation and severity. The pathogenesis of S. aureus infection is a complex process involving a diverse array of secreted and surface - associated virulence determinants that are coordinately expressed at different stages of infection (Loughman et al., 2009). Escherichia coli is a genetically diverse species that causes a variety of infections which fulfill many or all of the proposed criteria for biofilmassociated infections (Kaper et al., 2004). Klebsiella pneumoniae is an opportunistic pathogen responsible for a wide range of nosocomial infections. One important factor associated with virulence in K. pneumoniae is its capacity to adhere to surfaces and form biofilms (Cruz et al., 2012). Bacteria often exist as sessile communities called biofilms which are exquisite structures caused by a genetically programmed developmental process. It is estimated that biofilms are involved in 65% of human bacterial infections, since cells in biofilms are 1000 times more resistant than cells in the planktonic state, making medical treatments fail (Shal? et al., 2011). Extracellular DNA (eDNA) plays a significant role in biofilm formation, as revealed by studies in several bacteria including E. coli (Nakao, 2012) and K. pneumoniae (Whitchurch et al., 2002); however, there is no definite proof on a cause - and - effect relationship between DNA release and biofilm formation (Beenken et al., 2012) or becomes a key component of the macromolecular scaffold in many different biofilms (Jakubovics et al., 2013). In rare cases eDNA has been shown to inhibit bacterial settlement. Therefore, it is possible that nuclease mediated eDNA degradation would therefore promote adhesion. Consequently, it is not clear whether microbial nucleases contribute to the gross biofilm structure in clinically relevant situations (Sheilds et al., 2013). 2 Chapter one : Introduction and literature Review All previous studies used both commercial bovine and recombinant human DNaseI in the disruption of medically important biofilms; whilst, extracellular nuclease of S. aureus (SNase) was used against biofilm of S. aureus themselves (Benenken et al., 2012b ; Kiedrowski et al., 2014). Thus, to date, the role of S. aureus exonucleases in biofilm of other bacteria remains unclear. However, in the present study SNase is used against biofilm of other bacterial species (viz. K. pneumoniae and E. coli). To address this, the following steps were undertaken : 1 - Investigating the negative impact of SNase extracted from S. aureus on K. pneumoniae and E.coli biofilm formation. 2 - Studying the preventive activity of SNase on the adherence of K. pneumoniae and E. coli on uroepithelial cell.

دراسة وبائية وسريرية لمرض الجيارديا Giardiasis في اطفال مدينة الشرقاط وضواحيها == Parasitic Study of Giardiasise Among Children in Sharqat City

اسم المؤلف: علي حمد طلال موسى الجبوري
اسم المشرف: حسين ساهر اسود العبيدي | عبد الله حسين عبد الله الجبوري
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة لبيان تاثير طفيلي الجيارديا المعوي على صحة الاطفال في قضاء الشرقاط لـ(250) طفل، تم اختيارهم بصورة عشوائية، اذ تراوحت اعمارهم من شهر - 15 سنة وللمدة ما بين شهر تشرين الاول 2006 لغاية شهر تشرين الاول 2007، تم فحص عينات الغائط من كل طفل بال

دراسة بيئية ونوعية لمشروع تصفية ماء الكرخ في الطارمية == An Ecological And Qualitativ Study for Al - Karkh Drinking Water Plane At Al - Tarmia

اسم المؤلف: احمد توفيق احمد المشهداني
اسم المشرف: رياض عباس عبد الجبار
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: نظرا لما لمياه الشرب من اهمية كبيرة في حياة الانسان وضرورة ان تكون هذه المياه ضمن مواصفات ومعايير خاصة, فضلا عن ظهور العديد من مشاكل تلوث مياه الشرب في مختلف مدن العراق. دعت الحاجة لاجراء هذه الدراسة لبيان مدى صلاحية مياه الشرب المنتجة من مشروع تصفية ماء | Owing to the drinking water of great importance in human life and it is important to be under the specifications and standards, as well as the emergence of many of the problems of contamination of drinking water in various cities of Iraq. Needed to conduct this study to demonstrate the viability of drinking water produced from the project filter water Karkh (which is the largest project the liquidation of water in the Middle East, it is feeds many of the cities of Baghdad, on the side of Karkh) and compare the specifications of Iraq and the global standard for drinking water in order to investigate the potential weaknesses in the project and study the possibility of the development of such large projects as a first step to improving the situation of drinking water in Iraq. So were the tests, physical, chemical and biological common to drinking water in the project and divided into four main stations, namely, (station socket raw water, sedimentation basins secondary sand filters, pumps, distribution of drinking water), including nine stations secondary starting of the month of October - 2010 to July - 2011.The results showed high values of turbidity, and electrical condactivety and pH, and dissolved oxygen, and biological dimond of oxygen, and alkalinity, and total hardness, calcium hardness, magnesium hardness, chloride, phosphate, nitrite, silica, fluoride, sodium, potassium, and heavy metals, namely, (iron, copper and cadmium, nickel, lead, chromium and zinc), and the total numbers of bacteria, and total coliform bacteria, the fecal coliform bacteria and E. Coli in drinking water for most of the stations in the Alsta spring and low concentrations in summer and autumn.Values for water temperatures between (4 - 37.5) c, as recorded differences significant between the months of winter and spring, summer and autumn, and the values of brownish discharge ranged between (250 - 0.3) NTU as it showed the differences of the moral high values of turbidity in the winter and fall in the summer and fall in each stage of the treatmant with it, and the values of electrical conductivity was between (520 - 895) Micro Siemens / cm 3. The water Alnehrualcherb in the project was registered as a weak base pH values between (9.7 to 1.7), and it was well ventilated, as the oxygen values were high in the winter months registered clear monthly variation ranging between (5.14 - 2.3) mg / L, either values biologicaldimond of oxygen were values between (4.4 - ND) mg / L and found that basal total stations ranged from every stage of the liquidation potential in increasing concentrations of dissolved oxygen, between (160 - 100) mg / L, and found that drinking water hardness too, as that the values of total hardness ranged between ( - 215 335) mg / L as suited their values directly proportional to both the calcium and magnesium hardness ranging between (197 - 139) and (31.472 - 16.128) mg / L, respectively, while for the values of the chloride ranged between (69 - 25) mg / l, while the fluoride has recorded values ranged between (15 - 0.05) mg / L, with values ranging between sulfate (169.2 - 90) mg / L, free chlorine residual values ranged between (4.18 - 1.1) mg / L, and materials for suspended solids ranged values between (84 - ND) mg / L proven contribution stages of liquidation, all in the rates of removal of different, either TDS fluctuated values between (545 - 315) mg / L, with log Aluminum values ranged between (0.98 - 0.01) mg / L, but for the potassium values ranged between (3.2 - 2.15) mg / L, and sodium values ranging between (6.31 to 3.21) mg / liter.As for the concentrations of heavy metals did not detect any significant concentration of heavy metals measured except for iron ranging between concentrations (9.2 to 8.1) mg / L, so after the industrial plants that contain the droppings of these metals. The nutrient values were recorded between nitrite (0.024 - 0.001) mg / Trosgelt nitrate values between (1.7 - 0.16) mg / l, recorded between ammonia (0.54 - ND) mg / L, placed at either phosphate values ranged between (0.26 - ND) mg phosphorus atom - phosphate / L, while for the values of silica fluctuated between values (5.12 to 5.1) mg atom of silicon - silica / liter. As for the study of signs bacteriological ranged the total numbers of bacteria in air through (24) hours between (555 - 0) × 10 cells / 1 ml, and ranged in numbers for a period of (48) hours between (1186 - 0) 10 × cells / 1 ml, while for the numbers of total coliform bacteria fluctuated between (1700 - 0) cells / 100 liters, and fecal coliform bacteria, they ranged (1100 - 0) cells / 100 ml, and the E.coli bacteria, the numbers fluctuated between (1100 - 0) cells / 100 ml. For to match the drinking waterproduced from the projects liquefaction research found matching the values and concentrations of brownish, and connectivity electrical and pH, and dissolved oxygen, and the requirement is vital for oxygen, and basic college, and chloride, and ten college, and magnesium, nitrite, phosphate, and the total numbers of bacteria, and the numbers of total coliform bacteria, and fecal coliform bacteria and E Coli, and silica with the standard specifications for drinking water the Iraqi and global at all stations.While the study results show non - conformity of calcium concentrations in this study with the standard specifications for drinking water of Iraq and the world.

عزل وتشخيص البكتيريا Bacillus thuringiensis ودراسة تاثيرها في بعض المقاييس الحياتية لذبابة ثمار القرعيات (Diptera : Tephritidae) Dacus ciliatus == Isolation And Identification of Bacillus Thuringiensis And Studying Its Effects On Some Biological Parameters For Dacus Ciliatus (Diptera : Tephritidae)

اسم المؤلف: ادريس صالح جليل
اسم المشرف: عدنان نعمة عبد الرضا العزاوي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: تضمنت هذه الدراسة عزل البكتيريا Bacillus thuringiensis من مصادر بيئية مختلفة في محافظة ديالى وتشخيصها بالطرق الكيموحيويه والوراثية والجزئية، اذ جمعت 51 عينة من ترب مختلفة وهي(زراعية وحديقة وغير زراعية) وكذلك من الماء، من خمسة اقضية في محافظة ديالى (بعقوبة | This study included the isolation of pathogenic bacteria Bacillus thuringiensis from different environmental sources in Diyala province, diagnosis biochemical and genetic partial ways, Mar 50 samples collected from different soils, namely, (agricultural soils and garden soils and non - agricultural soils) as well as from the water, from five districts in the province of Diyala (Baquba, Khalis, Muqdadiyah, Khanaqin, Balad Ruz) for the period from 1 \ 9 \ 2014 to 20 \ 11 \ 2014. Showed the results of biochemical diagnosis and the presence of four samples and by percent 8% It was of a negative for transplantation bacterial and 46 sample growth by percent 92% It was of a growth positive for transplantation bacterial and found that 41 samples of which by percent 82% belong to the genus Bacillus and the species of Bacillus thuringiensis and the percentage of bacteria isolated of agricultural soils 94% and soils garden 93% and water 75%, and soils non - agricultural 44%. The pathogenic bacteria Bacillus thuringiensis of positive bacteria a gram dye and spore forming and The most of the natural habitat this bacterium is soil and it is capable of producing the diversified varieties of crystalline proteins with insecticide property. All isolates were tested for this bacteria that which were able growth on the media selective Acetate Sodium Selective and the production of insecticidal crystalline proteins (ICP) When staining by dye Coomassie brilliant blue (CBB) to conduct genetic partial diagnosis using several extraction (Mini gDNA Bacteria kit) processed by Bioneer company was the purity of DNA extracted ranging between (20.1 - 1.22) for all selected isolates, have been diagnosed with the bacteria at the level of genus and species Using the gene 16S rDNA with a molecular weight of 380 base pairs where the results showed polymerase chain reaction (PCR) that all isolates contain this gene and the same molecular weight which is 380 base pairs, also was diagnosed isolates containing the gene component crystalline proteins which cry a e molecular weight 200 base pairs using primer a specialist for this gene in polymerase chain reaction showed the results of gene doubled that all isolates contained the gene and the same molecular weight of 200 base pairs. After making sure of pathogenic bacteria as belonging to the genus Bacillus and species Bacillus thuringiensis according to their chemical diagnostics biochemical and diagnostic genetic partial, attended different concentrations They (1, 2, 3, 4, 5) g/L of thebio preparation for these bacteria to study their impact on the different roles of Cucurbits fruit fly Daus ciliatus. Has been tested the effect of different concentrations of bio - preparation of these bacteria isolated from soil and water on the roles of this insect which eggs and larvae and pupa and AdultsThe results of test the effect of bio - preparation of these bacteria on the eggs of age (1 - 2) Since the highest rate of hatchling (3.2) and by (89%) at a concentration of 1 g / l of bio - preparation and lowest hatchling (0.6) and by (19%) at a concentration of 5 g / L of bio - preparation comparison with control (3.2) and by (96%) for the isolated bacteria from soil Since the highest rate of hatchling (3.1) and by (92%) at a concentration 1 g / L of bio - preparation and lowest hatchling (2.1) and by (62%) at a concentration of 5, 4 g / L of bio - preparation comparison with control (3.2) and by (96%) for isolated bacteria from water, and on The last larval phase, The highest the value of the rate of highest (2.0) and by (99%) at a concentration of 5 g / L of bio - preparation and The less the value of the rate of loss (0.5) and by (26%) at 1g / L of bio - preparation concentration compared with the control treatment (0.07) and by (%3) for isolated bacteria from soil.reached its highest value the loss rate (1.2) and by (57%) at a concentration of 5 g / L of bio - preparation and less the value of the loss rate (1.0) and by (6%) at a concentration 1 g / L of bio - preparation comparison with control (0.07) and by (3%) for the bacteria isolated from water and on pupa age(6 - 7) day, reaching the highest rate of emergence of pupa (3.2) and by (92%) at a concentration of 1 g / L of bio - preparation and less the emergence of pupa rate (0.4) and (13%) at a concentration of 5 g /L of bio - preparation comparison with control (3.2) and by 96% for bacteria isolated from soil reaching the top of the emergence of pupa rate (3.1) and by (92%) at a concentration of 1 g / L of bio - preparation and less the emergence of pupa rate (2.1) and by (62%) at a concentration of 5 g / L of bio - preparation comparison with control (3.2) and by (96%) for bacteria isolated from water. and on kill adult, The highest killing rate for males (1.6) and by (32%) and females (1.5) and (30%) at a concentration of 5 g / l of bio - preparation and less killing of the male rate (0.5) and (10%) and females (0.4) and by (13%), comparison with control males (0.1) and by (2%) and females (0.1) and by (2%) for the isolated bacteria from soil. The highest kill the male rate (0.8) and by (32%) and females (0.7) and (30%) at a concentration of 5 g / L of bio - preparation, and less killing of the male rate (0.2) and (4%) and females (0.4) and by (13%) at a concentration of 1 g / L of bio - preparation comparison with control males (0.1) and by (2%) and females (0.1) and by (2%)for the isolated bacteria from water and one female productivity of egg reached its highest production rate eggs 84.5 eggs per female at a concentration of 1 g / L of bio - preparation, and less egg production rate of 54.5 eggs per female at a concentration of 5 g / L of bio - preparation comparison with control eggs productivity rate of 87.5 egg female for isolated bacteria from soil reaching its highest egg production rate (85%) eggs per female at a concentration of 1 g / L of bio - preparation, and less egg production rate (79.5%) eggs per female at a concentration of 5 g / L of bio - preparation, compared with treatment control productivity rate of whites (85.5%) eggs per female for isolated bacteria from the water

تقييم فعالية مستخلصات مستنبت القمح Triticum aestivum في بعض الانواع البكتيرية المسببة لاخماج مختلفة == Evaluation The Activity of Germinated Wheat Extractions Triticum Aestivum on Some Bacterial Species That Cause Different Infections

اسم المؤلف: اسماء عيسى محمود
اسم المشرف: رشيد حميد حسن
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: The study had included isolation and diagnosis of three hundred samples for both sexes their ages were between (1 - 60) years of the inpatients and outpatients in Tikrit Teaching Hospital and General Samarraa Hospital during the period from 1/9 / 2011 to 1/3/2012. The infections were distributed among the burns infections, the wounds, urinary tract infections (UTI) and female genital tract infections (High vagina). This study clarified that (192) sample from the total samples showed bacterial growth in percentage 64%. The results of this study indicated to the proportion 58% with (UTI) by (87) bacterial isolation were produced from (150) isolation. The percentage of burns infections were 81.81% from (55) sample which distributed among the first, second and third class burns. The burns of second and third class gave bacterial growth in a proportion 100% while the first class burns gave 64.28%. Concerning the wounds infections, bacterial growth was 62.9% from (62) sample. The infections proportion of (H.V) was 63.63% from (33) sample. The results of the current study showed that the infections in females were higher that in males with respect to (UTI), while there was not considered difference between the males and females about the burns and wounds infections. Differently, the (H.V) infections were just for the females in the ages between (18 - 45). It was clear according to the results of diagnosis that Escherichia coli had the highest proportion of isolations 25.52% from the total isolates followed by Staphylococcus aureus 18.23% Pseudomonas aeruginosa 15.63%, both Klebsiella pneumoniae and Proteus miribilis 10.42% Staphylococcus epidermidis 4.69% Citrobacter freundii 4.17%, Staphylococcus saprophyticus 3.64% both Serratia marcescens and Enterococcus faecalis 2.6%, Streptococcus pyogenes 1.56% and Staphylococcus intermedius had the least percentage 0.5% by one from (192) isolate. The sensitivity of these isolates was tested by (10) antibiotic which included quinolones, aminoglycosides, beta - lactams and tetracyclines antibiotics. Among these antibiotics imipenem and ciprofloxacin were more effective, while the other antibiotics were different either active or not active at all.The wheat germinating was used in this study with the ages (36 - 48) hour and its activity was tested by using some bacterial kinds. After completing several quantitative and qualitative tests on this germinating, the carbohydrates proportion was between 67 - 70%, the protein proportion by folin method was between 10 - 8%, the lipids proportion was 1.5%.Some mineral elements in germinated wheat were estimated which include Calcium, Iron, Magnesium, Potassium, Copper, Manganese, Zinc, Selenium, and Sodium element, active materials were also estimated in the wheat germinating extracts which had inhibitory effect on the microbiology. It was clear by the tests that the germinating contains a lot of Phenolic compounds, Terpeniods, glycosides and steroids. These materials were extracted by using some extracts included the cold water extracts and hot water extracts, alcoholic extracts (ethanol and methanol) and phenolic extract. All the extracts prepared in concentrations (25, 50, 100, 200) mg/ ml. This study showed that the phenolic extract had high effect on the bacterial kinds in comparison with the methanolic and ethanolic extracts at (200) mg/ ml concentration while the cold water extract had less effect on these bacterial isolates in comparison with the above extracts, finally the hot water extract had no inhibitory effect against any isolated bacterial type with all the concentrations. The inhibitory action of these extracts was compared with some antibiotics as a control sample. These antibiotics represents different groups of antibiotics and which contained Imipenem, Ciprofloxacin, Gentamicin, Ceftazidime in addition to Augmentin.In this study the minimum inhibitory concentration (MIC) of the fenolic, alcoholic and watery extracts was determined and the concentration was different according to the extract type, the bacterial type and the isolation site.
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