البحث — المستودع الرقمي العراقي

المستخلص: استخدم فيها 30 ارنبا ذكرا حيث قسمت الى ثلاثة مجاميع متساوية بالعدد كل مجوعه تحوي على عشرة ارانب اثنان منها تم اختيارها كواهب لنخاع العظم والثمانيه الاخرى كانت مستلمه, وكانت المجموعه الاولى هي مجموعه سيطرة وقد اعطيت المحلول الملحي الطبيعي ,والمجموعه الث | Thirty male rabbits were randomly divided into three groups as following : Group one (sham group) consists of ten male rabbits, two of them were used as donors and other eight were administered 12.5ml/kg/BW normal saline (N.S) orally for ten days. At Zero time of the experimental the collection of blood samples (10ml) was carried out on each animal from the heart (cardiac puncture) and by a sterile syringe 22 Gage needle. The (10ml) amount of blood was divided into two parts. (5ml) was deposited into tubes without anticoagulant and then refrigerated for a maximum of 12h and centrifuged at (5000 rpm) for 15 minutes these serum samples were stored in polyethylene eppendorff tubes at - 20?C Estimation of Total interleukin 2 in serum by using an Enzyme - Linked Immunosorbent Assay (ELISA). The 2nd sample was also (5ml) taken for performing hematological test to measure the WBCs account and their differential, RBCs account, Hb & P.C.V. At Day10 of experimental was collecting the blood as similar procedure at zero time and did a similar examination before operation. At Day11 of the experimental an allogeneic Intra - bone - marrow injection of bone marrow transplantation aspiration method (IBM - BMT) AM was carried out and rabbits were re administered with normal saline after surgical operation until Day21 of experimental. Following that rabbits were anesthetized with Ketamine (20 mg/Kg/BW) and xylzine (9 mg/Kg/BW) intra muscular. Before bone aspiration for bone marrow histological test the smears were stained with Giemsa stain. At the end of this experimental study of there was collection of blood as a same procedure at zero time and DAY10 then made the same test to estimate of blood values and interleukin 2. Then all recipients’ rabbits were sacrificed and bled to death to obtain the Kidney and liver, which were dissected out, trimmed and processed for the histological study with used Heamatoxyline and Eosin Stain. Group two (treated with 12.5ml/kg/BW cyclosporine orally administrated) and same protocol of the sham group was applied except when using (treatment with 12.5ml/kg/bw cyclosporine orally administrated) replacing the normal saline. Group three (treatment with 25ml/kg/BW cyclosporine orally administrated) and same protocol of sham group was applied except when using (treated with 25ml/kg/BW cyclosporine orally administrated) replacing the normal saline. The results of the present study Conclusions cyclosporine effects on the bone marrow, immune system because of decrease of WBCs, lymphocytes, neutrophils and interleukin 2.The study Conclusions cyclosporine caused many adverse effects, where by it caused severe liver and kidney; therefore it was considered predisposing factor to infection with several diseases as well as it is considered the main cause of disorders in renal function renal dysfunction. The results of the present study Conclusion the surgical operation of intra - bone - bone marrow transplantation more safe than other ways of intra vinous bone marrow transplantation also the study the operation increase the hematopoietic cells

الصفحات الاولى:

مكان الجامعة: البصرة

اللغة: الانكليزية

الجامعة: جامعة البصرة - كلية الطب البيطري - قسم الجراحة والتوليد البيطري

الدرجة: ماجستير

الموضوع الدقيق: الجراحة

السنة: 2014

الموضوع العام: الطب البيطري

اسم المشرف: Abdulbari A. Alfaris Alaa A. AL Sawad

اسم المؤلف: عادل جاسب عويد

تاثير نخاع العظم وحامض الهيالورونيك في اصلاح وتر القابضة الاصبعية السطحية المرفو في الحمير Effect of Bone Marrow And Hyaluronic Acid Bioscaffold of Repair of SDFT Tenorrhaphy In Donkeys

المستخلص: The study was designed to assign the effectiveness of bone marrow and hyaluronic acid to repair of experimentally induced transected of the SDFT in donkeys by macroscopical, ultrasonical and histopathological evaluation.A total of 18 clinical healthy donkeys of both sexes with the age of 2 - 3 years weighting of 70 - 130 kg (mean ±SE, weight 97.77 ± 8.11 kg), were used for this study. The animals divided randomly into three equal groups (six animals/groups), the (group A) was used as a control group. The treatment groups were classified into and the bone marrow group (group B) and the hyaluronic acid group (group C) and the animals were generally anesthetized.In all animals of this study a mid - metacarpal linear skin incision was made in the palmer aspect of the left forelimb, the SDFT then was transected transversely and then the cut ends of the tendon were approximated by (0.2) Nylon suture and skin closure by using (0.2) silk suture. In group (B), two ml of bone marrow was applied to the sites of anastomoses tendon. In group (C), use a two ml of sodium hyaluronate on the sites of anastomoses tendon.Macroscopical examination showed adhesions between the tendon and surrounding tissues which were severe in the group (A) than in group (B) and lesser than that in group (C).Histopathological findings with the group (A) showed edematous fluid and collagen fiber in 30 days postoperatively and the presence of the edema and irregular collagen fiber were observed at day 60th. In group (B) showed a marked increase in neo blood vessels (angiogenesis) and collagen fiber in 30 days postoperatively and observed a moderate number of new blood vessels and collagen fibers at 60 days postoperatively.In group (C) demonstrated a numerate new blood vessels ,the collagen fiber and edema at 30 days postoperative and the prominent fibroblast at 60 days postoperative.Ultrasonographic examination for the tendons of the group (A), 30 days postoperatively showed the presence of marked fluid in SDFT with anechoic over the site of operation (dark area) while, 60 days postoperatively there is moderate fluid the SDFT appear as area of anechoic to hypo echoic.In tendons of group (B) in 30 days postoperatively notice the presence of lesion in a tendon, which appear as hypo echoic area with slower to demonstrate evidence of healing on ultrasound. In 60 days post operatively there is corresponding longitudinal fiber, alignment grade with hyper echoic appearance of operation area. In tendons of group (C) in 30 days postoperatively ultrasonography shows the presence of moderate fluid and the hypo echoic area within the SDFT. In 60 days postoperatively notice the presence of mild fluid in the site of operation that reflected as hypo echoic to hyper echoic area.The results of this study demonstrated the effectiveness of (Bone marrow) in acceleration the process of tendon healing and reducing fibrous adhesions that occur after the exposure of the tendon to injury compared to the (Hyaluronic acid), which has the same effect but to a lesser extent