تاثير نخاع العظم وحامض الهيالورونيك في اصلاح وتر القابضة الاصبعية السطحية المرفو في الحمير == Effect of Bone Marrow And Hyaluronic Acid Bioscaffold of Repair of SDFT Tenorrhaphy In Donkeys
Author name:
حميد عبد غاطي
Supervisor name:
عبد الباري عباس ساهي الفارس
General topic:
Veterinary Medicine
Specific topic:
Surgery
Degree:
Master
University:
University Of Basrah - College Of Veterinary Medicine - Department Of Surgery And Veterinary Obstetrics
Language:
English
University location:
Basrah
First pages:
21T499 - p.pdf
Abstract:
The study was designed to assign the effectiveness of bone marrow and hyaluronic acid to repair of experimentally induced transected of the SDFT in donkeys by macroscopical, ultrasonical and histopathological evaluation.A total of 18 clinical healthy donkeys of both sexes with the age of 2 - 3 years weighting of 70 - 130 kg (mean ±SE, weight 97.77 ± 8.11 kg), were used for this study. The animals divided randomly into three equal groups (six animals/groups), the (group A) was used as a control group. The treatment groups were classified into and the bone marrow group (group B) and the hyaluronic acid group (group C) and the animals were generally anesthetized.In all animals of this study a mid - metacarpal linear skin incision was made in the palmer aspect of the left forelimb, the SDFT then was transected transversely and then the cut ends of the tendon were approximated by (0.2) Nylon suture and skin closure by using (0.2) silk suture. In group (B), two ml of bone marrow was applied to the sites of anastomoses tendon. In group (C), use a two ml of sodium hyaluronate on the sites of anastomoses tendon.Macroscopical examination showed adhesions between the tendon and surrounding tissues which were severe in the group (A) than in group (B) and lesser than that in group (C).Histopathological findings with the group (A) showed edematous fluid and collagen fiber in 30 days postoperatively and the presence of the edema and irregular collagen fiber were observed at day 60th. In group (B) showed a marked increase in neo blood vessels (angiogenesis) and collagen fiber in 30 days postoperatively and observed a moderate number of new blood vessels and collagen fibers at 60 days postoperatively.In group (C) demonstrated a numerate new blood vessels ,the collagen fiber and edema at 30 days postoperative and the prominent fibroblast at 60 days postoperative.Ultrasonographic examination for the tendons of the group (A), 30 days postoperatively showed the presence of marked fluid in SDFT with anechoic over the site of operation (dark area) while, 60 days postoperatively there is moderate fluid the SDFT appear as area of anechoic to hypo echoic.In tendons of group (B) in 30 days postoperatively notice the presence of lesion in a tendon, which appear as hypo echoic area with slower to demonstrate evidence of healing on ultrasound. In 60 days post operatively there is corresponding longitudinal fiber, alignment grade with hyper echoic appearance of operation area. In tendons of group (C) in 30 days postoperatively ultrasonography shows the presence of moderate fluid and the hypo echoic area within the SDFT. In 60 days postoperatively notice the presence of mild fluid in the site of operation that reflected as hypo echoic to hyper echoic area.The results of this study demonstrated the effectiveness of (Bone marrow) in acceleration the process of tendon healing and reducing fibrous adhesions that occur after the exposure of the tendon to injury compared to the (Hyaluronic acid), which has the same effect but to a lesser extent
