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تاثير المجال الكهرومغناطيسي في بعض صفات النمو والحاصل والانقسام الخلوي لنبات العصفر Carthamus tinctorius L == The Effect of The Electromagnetic Field On Some Growth Characteristics And Yield And Mitotic Index For Carthamus Tinctorius L

اسم المؤلف: مروة قيس ابراهيم العبيدي
اسم المشرف: ماهر زكي فيصل الشمري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اولا : - التجربة الحقلية : اجريت التجربة في الحقل التابع لقسم علوم الحياة - كلية التربية للعلوم الصرفة - ابن الهيثم، وصممت باستخدام تصميم القطاعات الكاملة المعشاة Randomized Complete Blocks Design (RCBD) حيث تم تعريض البذور قبل الزراعة لمجال كهرومغناطيسي | First : - Field experience : the experiment was conducted in the Department field of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using design Full sectors Randomized Complete Blocks Design (RCBD) where the seeds exposing to electromagnetic field in the Department of Physics - Electrical Laboratory - College of Education, Pure Science - Ibn al - Haitham before planting them in five intensities (0, 5, 10, 15, 20) mT and two (1.2 and 3) an hour for each intensity to see the electromagnetic field effect in some of the qualities of growth and yield plant Safflower the results were as follows : The effect of electromagnetic field strength was positive traits and was the highest increase in exposure to the intensity of 10 mT for each of the (percentage of germination, root size of the second date, plant height, leaf area, leaf area guide, the dry weight of the root system at the time the second, fresh weight of shoots at the first appointment, the average absolute growth, the sustainability of biomass, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (81.21%, 18.33 cm 3, 93.37 cm, 71.29 cm 2, 1.187, 8.21 g.plant 1, 155.13 g.plant - 1, 1.76 g.yum 1, 691.67 g, 2.03%, 0.733%, 2.47%, 12.96%, 56.96 spad, 44.55 Nora.plant - 1, 46.60 g.plant - 1, 5.35 g, 161.80 kg. acres - 1, 38.74 mg. plant - 1, 12.09%, 13.50%), respectively, except for the size of the root first date, fresh weight of the total root for the first two dates, and second, the dry weight of the root system in the first date, fresh weight of shoots in the second deadline, the dry weight of shoots for the first two dates, and second, the specific weight of the oil had the highest increase at exposing the seeds to the intensity of 15 mT and average (15.16 cm, 15.12 g. Plant - 1, 26.00 g plant - 1, 3.98 g plant - 1, 411.08 g.plant - 1, 20.03 g. Plant - 1, 69.15 g. Plant - 1, 1.069%, respectively) and the highest increase of the proportion of total oil when exposure to the severity of 5mT an average 33.94%. The effect of the length of time in field was significant in most of the qualities which gave the term two highest increase with the exception of germination percentage was the highest increase in exposure for a period of three hours, with an average (60.79%, 91.01 cm, 67.51 cm 2, 1.124, 131.93 g. Plant - 1, 337.61 g. plant - 1, 1.68 g. Day - 1, 1.92%, 12.04 g. plant, 55.99 Spad, 146.06 kg. dunams - 1, 4.77 g, 40.23 mg. plant - 1, 28.03%) for each of the (germination percentage, plant height, leaf area, leaf area guide, fresh weight of shoots in the first date, fresh weight of shoots in the second deadline, the average absolute growth, nitrogen, protein, chlorophyll, yield seeds, 100 - seed weight, carbohydrate content, the total percentage of oil). As was the overlap between field strength and duration significant effect on most of the traits with the superiority of treatment (10 mT for two hours) on the rest of the other transactions for each of the (germination percentage, the size of the root of the first two dates, and the second, plant height, number of branches, leaf area, area guide paper, fresh weight of the root system of the first two dates and II, , dry weight of the root of the first two dates, and the second, fresh weight of shoots for the first two dates, and second, the dry weight of shoots for the first two dates, and second, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, He yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (39.33%, 16.50 cm, 19.50 cm, 103.86%, 17.00, 79.83 cm, 1.330, 16.43 g.plant - 1, 29.20 g. plant - 1, 4.16 g.plant - 1, 10.48 g.plant 1, 184.06 g. plant - 1, 416.33 g. plant - 1, 22.35 g. plant - 1, 73.25 g. plant - 1, 2.10%, 0.800 %, 3.04%, 13.15%, 60.31 spad, 49.14 Nora.plant - 1, 53.37 g. plant - 1, 6.14 g 185.97 kg. acres - 1, - 1.plant 47.81 mg, 2.28%, 14.25%), respectively, with exception each of the average of the absolute growth and sustainability of biomass, where the highest increase in exposure to the intensity of 10 mT for a period of three hours and average (1.85 g.yum 1, 724.85 g.yum), respectively, and total percent of the oil was the highest increase in exposure to the intensity of 5 mT and for three hours and an average of 37.28% and the intensity of 15 mT for a period of three hours, specific gravity of oil and an average of 1.081%Second : - laboratory experiment : experiment was conducted in the laboratory of physiology of the plant Higher Studies - Department of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using the full random design Completely Randomized Design (CRD) to study the electromagnetic field effect five intensities (0, 5, 10, 15, 20) mT and two (1.2 3) per hour on the severity of the cell division of the safflower plant results were as follows : The results showed that there is a positive effect of the electromagnetic field on the traits and was the highest increase at exposing the seeds to the intensity of 10 mT for each of the (number of dividing cells of the tops of developing the parties to the roots, the total number of cells to the tops of the parties to the roots, and guide cell division developing the tops of the parties to the roots) and average (156.00% 1143.00%, 12.17%), respectively. The effect of duration of exposure, and had a significant effect on the average number of total cells to the tops of the parties to the roots reached 1042.20% when the exposure for two hours.The effect of overlap between the severity and duration of the electromagnetic field significant was effect in all cellular qualities and was the highest increase in exposure to the intensity of 10 mT for a period of one hour and average (167.00%, 13.52%) for each of the number of dividing cells developing the tops of the parties to the roots and guide cell division to the tops of developing the parties to the roots, The total number of cells to the tops of the parties to the roots was the highest increase in exposure to the intensity of 10 mT for two hours and an average of 1203.50%.

التحري عن جين الاوتولايسين في المكورات العنقوديه البشروية المقاومة لمضاد الفانكومايسين == Autolysin Gene Detection In Vancomycin Resistant Staphylococcus Epidermidis

اسم المؤلف: مريم خميس عبد ربة بريس
اسم المشرف: مي طالب فليح
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: من مجموع مئة عينة سريرية جمعت من مصادر مختلفة شملت الحروق والدم والجروح ومسحات انفية, تمكنت 90 عزلة من النمو على اكار المانيتول الملحي , ومن بينهم 40(44.4 %) عزلة عائدة لبكتريا المكورات العنقودية المنتجة للانزيم المخثر للبلازما و50 (55.5%) عائدة لبكتريا ا | Out of one hundred clinical samples were taken from different sources which include burns, blood cultures, wounds and nasal swabs infections ; 90 isolates developed growth on mannitol salt agar. Among these, 40 (44.4%) were Coagulase positive (Staphylococcus aureus) isolates, 50 (55.5%) belong to coagulase negative staphylococci in which Staphylococcus epidermidis isolates were 30(60%). The pattern of antibiotic susceptibility of Staphylococcus epidermidis isolates to 12 antibotics (Amoxiclav, Ceftazidim, ciprofloxacin, clindamycin, Erythromycin, Gentamycin, Imipenime, Penicillin G, Tetracycline, Rifampin , Methicillin, and Vancomycin) were determined using disc diffusion method. The results revealed that resistance to Penicillin G10 and Amoxiclav (Amoxicillin - clavulanic acid) were 100%, Methicillin were 93%, Erythromycin were 90%, Gentamycin and Clindamycin were 70%, Tetracycline and ceftazidim were 75%, Ciproflaxacin were 60%, Rifampin were30%. 95% of S.epidermidis isolates were sensitive to Imipenim and 5% of them were intermediate resistant, while these isolates showed 90% sensitivity to vancomycin. 19 isolates were multidrug resistance. Minimum inhibitory concentration of S.epidermidis isolates to vancomycin, was determined. The results revealed that (12) S.epidermidis isolates (40 %) were vancomycin resistant, the MIC of them were between 256 ?g\ml and 32 ?g\ml, (4) S.epidermidis isolates (13.3%) were intermediate resistance, the MIC to 3 of them were 16 ?g\ml and the last was 8 ?g\ml. Some virulence factors of VRSE and VSSE were detected including the hemolysin, protease, lipase and urease. The S. epidermidis isolates were produce hemolysin, protases, lipase and urease, were 100%, 100%, 25%, 100% respectively in VRSE, while in VSSE 100%, 100%, 60%, 100% respectively. The isolates were subjected to polymerase chain reaction (PCR) technique in monoplex pattern to amplify resistant incoding gene : the vanA, vanB and autolysine gene aae gene. The results by this study showed that 12 (40%) S. epidermidis isolates gave the implicone size (1030 base pair) of the vanA gene. However the results of MIC and PCR were similar but no any isolates gave product for presence of vanB gene. All S.epidermidis were able to produce implicone size(858bp) of aae gene. The effect of vancomycin resistant S. epidermidis on cell autolysis activity was detected by whole cell autolytic assay.The results revealed that there was significant difference among three isolates, the VSSE isolate (S.epidermidis 22) have the highest autolytic activity in the presence of antibiotic, followed by the VRSE isolate (S. epidermidis 1) and the VISE isolate (S. epidermidis14) which was the lowest autolytic activity with the presence of antibiotic. The result of transmission electron microscope (TEM) showed that the VRSE isolates (S.epidermidis 1) have thicker cell wall followed by VISE (S.epidermidis 14) isolates.However, the VSSE (S.epidermidis 22) didn't showed any cell wall thickening.

تاثير المستخلص الكحولي لنبات الخباز (Malva sylvestris ) في فسلجة ونسجية الكبد والخصية في ذكور الفئران البيض == Effect of Alcoholic Extract of Mallow (Malva Sylvestris) On Physiology And Histology Liver And Testis In Albino Male Mice

اسم المؤلف: بسمة علي جاسم
اسم المشرف: مختار خميس محمد سعيد
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: صممت هذه الدراسة للتعرف على مدى تاثير المستخلص الكحولي الخام لنبات الخباز Malva Sylvestris في الكبد والخصى لذكور الفئران البيض نسجيا, وكذلك فسلجيامن خلال دراسة مستوى انزيمي Glutamate - oxaloactate - transaminase (GOT) وGlutamate - pyruvate - transaminase( | This study was designed to investigate the effects of crude alcoholic extract of Malva sylvestris on the histology of liver and testes in male albino mice, as well as levels of the GOT, GPT enzymes and testosterone hormone. Seventy two adult male albino mice were used in this study. The animals were randomly divided into four main groups, the first group was given distilled water (control), while the other three groups were given (100, 300, 500 mg /kg) of crude extract. These main groups were subdivided into three subgroups, according to the period of treatment (2, 4, 6, 8)weeks. Blood samples were collected every two weeks for physiological study, and samples of the liver and testes were collected for histological study. Treating with crude alcoholic of Malva sylvestris led to no significant increase in the mean of the body weight except the concentration (500 mg /kg). There was significantly increase in the liver weight particularly in(500 mg /kg) for eight weeks of exposure, also there was significantly increase in the average of the testes weights treated with Malva sylvestris extract specially in (300, 500 mg /kg) for all periods of treatment compared with control group. Malva sylvestris extract coused significantly decrease in both GOT, GPT. there fore the value of GOT, GPT considers as concentration - period exposure dependent the highest value of GPT was (300 mg /kg) for two weeks (2. 25±228. 04) IU/L compared with control group (3. 03±226. 02) IU/L. and the lowest value of GPT was (500 mg /kg) for eight weeks (4. 52±192. 27)IU/L compared with control group (4. 06±208. 77) IU/L the highest value of GOT in (100 mg /kg) reached (4. 10±63. 00) IU/L for two weeks compared with control group (1. 25±71. 23) IU/L. the lowest value was (2. 77±49. 83) IU/L in (500 mg /kg) with eight weeks comparison with control group (1. 18±69. 02) IU/L. all concentration of Malva sylvestris extract made positive changes in the value of testosterone with all periods of exposure particularly in (300 mg /kg) compared with control group. There were many histopathological alterations appeared in the liver tissue among treated groups which caused by the effects of Malva sylvestris extract represented by congestion, disarrangement of hepatic cords, beginning of necrosis of hepatocytes, infiltration of leukocytes, narrowing of sinusoids, increase the number of binuclei cells, degeneration, appearance of giant cells, all these changes were reversible. Malva sylvestris extract made beneficial histopathological changes in the testes tissue in all treated groups like increase in numbers of ledyig cells specially concentration (500 mg /kg) reached (11.37± 0.80) IU/L with two weeks comparison with control group(5.38 ±0.34) IU/L, and increase in the thickness of the walls of seminiferous tubules specially concentration (500 mg /kg) reached(29.46± 1.39) IU/L with compared with control group(20.52±2.40) IU/L. In addition the role of extract in significant decline in the diameters of seminiferous tubules in all treated groups compared with control group

نوعية البويضة والتكوين الجنيني بعد الاعطاء الفموي للسبيرماكس لاناث الفئران : موديل تجريبي للبائن == Oocyte Quality And Embryonic Development After Oral Administration of Speramax® In Female Mice : Experimental Model For Mammal

اسم المؤلف: هبة صاحب حمزة
اسم المشرف: سعد صالح الدجيلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الاجنة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Background : Speramax® has been found to play an important role in sperm function characters and males reproductive performance with no studies on its effects on the oocyte maturation and embryonic development in females.Objective : The goal of the thesis is to examine the effect of Speramax ® on oocyte maturation. And to emphasize the effect of Speramax ® on ova quality, embryonic development and newborn. Materials and Methods : Speramax ® treated by oral administration for 1, 2 and 4 weeks. A hundred and twenty six female mice were randomly divided into four groups, the first group was treated without Speramax® with superovulation(SUO) while the second group were treated by Speramax® with SUO and the third group were superovulated only and the fourth group was not treated and spontaneously ovulated (SPO) and considered the control group(thirty two mice). Results : The results indicated that the treatment with Speramax® showed a positive effect on oocytes maturation in vivo. There was a highly significance (p? 0.0001) improvement in number of mature oocytes following treatment with Speramax® in SPO and SUO mice compared with SPO and SUO mice not treated with Speramax®. The embryonic developmental rate after 24 and 48 hours of mating in treated groups with Speramax® was significantly (p? 0.05) higher than those of SPO and SUO mice too.The study showed that the quantity and quality of embryos generated from the treated groups were superior to that of untreated groups.Conclusion : It was concluded that the treatment by Speramax® has a great improvement on oocyte maturation, early embryonic development and embryo grading quality of mice embryos with an increase in the numbers of mice newborn.

الفعالية المضادة للاحياء المجهرية والانزيمية للفطريات المستنبتة المعزولة من نبات الاس Myrtle (MyrtuscommunisL.) == Antimicrobial And Enzymaticactivity of Endophyticfungiisolatedfrommyrtle (Myrtuscommunisl.)

اسم المؤلف: هدى محمد كاظم ديوان
اسم المشرف: بتول زينل علي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: استهدفت الدراسة الحالية عزل وتشخيص الفطريات المستنبتة في اوراق نبات الاس Myrtus communis واختبار فعاليتها المضادة للاحياء المجهرية وفعاليتها الانزيمية. - اظهرت نتائج عزل الفطريات المستنبتة في 500 قطعة اوراق للحصول على عدد كلي للعزلات مساويا الى 99 عزلة ف | The present study was aimed to isolate and identify endophytic fungi from leaves of myrtle Myrtus communisand study their antimicrobial and enzymatic activities. - Results of fungal isolation from 500 leaves fragments revealed isolation of 99 endophytic fungi represented a total colonization frequency (CF) of 20.4%. These fungi were related to 23 species or isolates, where Aspergillus spp. prevailed over all others (11 species) with CF of 14% for all Aspergilli, Aspergillusniger and Aspergillusflavus showed the highest CF 7.2% and 3% respectively, other Aspergilli represented Aspergillusparvulus (CF 1.6%), Aspergilluscandidus (0.4%), Aspergillusraperi, Aspergillusclavatus, Aspergillusglaucus, Aspergillusornatus, Aspergillussclerotioniger, Aspergillusterreus and Aspergilluswentii were all showed CF of 0.2%.Other isolates fungi from leaves fragments were four morphologically different isolates of Penicillium with 0.4% CF for each. Additionaly, isolates of Cladosporium sp. (2%) and Cladosporiumcladosporoides (0.4%), as well as Cunninghamella sp., Drechsleraaustraliensis, Alternariaraphani, which showed 0.2% CF for each, Paecilomyces variotii (0.6|%), and different isolates of sterile fungi (White and Hyaline colonies) were also obtained. - Study the antagonistic activity of isolated endophytes using dual culture method against five plant pathogenic fungi(Fusarium oxysporum, Fusarium graminearum, Fusarium sp., Macrophomina phaseolinaandRhizoctonia solani)taking into account the percentage of inhibition of pathogenic fungi, results revealed variation of antagonistic activities between endophytes and pathogens. Different cases of antagonism were also appeared ranged from mycoparasitism of the endophytes on pathogens or vice versa, to competition between the two fungi and to the appearance of inhibition zone between them, this result may indicates the affinity of using some isolated endophytes in biological control applications against or controling fungal plant diseases. - Effect of ethylacetate extracts of culture broth of active endophytes shown in the last experiment against two bactria E.coli, S.aureus and the yeast Candida albicans using disc diffusion method, where filter paper discs were imprignated with different concentrations of fungal broth extracts. Results showed variation of inhibitory activity against bacteria with different endophytes, bacteria and conc. of the extract. Regarding the effect onC.albicans and filamentous fungi, most fungal broth extracts showed no inhibitory activity against yeast and no extract as well showed inhibitory activity on filamentous fungi. These results give indication thatbroth extracts contain antibacterial compounds. - Addition of 20% crude fungal broth of some endophytes to the culture medium showed significant inhibitory activity against filamentous fungi which did not respond to broth extracts, percentage of inhibition ranged between 0 - 77.08% and maximum inhibitory activity was shown by Penicillium sp. Isolate. These results give indication that some of crude filtrates contain antifungal compounds. - Analysis of broth extracts of some endophytes using TLC technique showed the content of extract of number of UV illuminated spots isolated on TLC pates having different Rf values, Bioautography showed inhibitory activity of some of these isolated spots against E.coli, S.aureus and the Filamentous fungus F.oxysporum. - Testing the extracellular enzymes activities of isolated endophyles on solid media, enzymes included amylase, lipase, pectinase, laccase, cellulase and protease showed variation in secretion of these enzymes, as well as, no endophytes showed the affinity of secreting all tested enzymes. Results also showed the high affinity of some endpohytes to secrete enzymes that could be use in multiple important applications after separation andcharacterizationof these enzymes.

التحري عن نوعية مياه الشرب لمحطتي معالجة مياه الحي والبشائر في محافظة واسط جنوبي العراق == Investigation of Drinking Water Quality In Al - Haay And Al - Bashaar Water Treatment Plants In Wasit Province Southern Iraq

اسم المؤلف: وسام باسم محمد التميمي
اسم المشرف: احمد جاسم محمد العزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: م اجراء فحوصات فيزيائية وكيميائية واحيائية لمياه الشرب في محطتي الحي والبشائر وعدد من المناطق التي تغذيها, شهريا للمدة من تشرين الاول 2013 الى شهر تموز 2014, عبر مراحل التصفية وصولا الى المنازل التي تقع على مسافات مختلفة عن مصدر التجهيز وبواقع نموذجين لكل | Physical, chemical and a biological tests were carried out and drinking water samples were collected from AL - Haay and AL - Bashaer water treatment plants and number of residential areas fed by these plants for period extended from October 2013 to July 2014, through the purification stages up to residential sites at different areas situated with various distances from the supplying source, two samples were taken monthly. Air temperature at sampling time was varied from 16°C to 42°C and from 10°C to 34°C, for water temperature at sampling time also. The results of pH values were within the allowable limits, ranging from 7.1in autumn to 8.1 in summer. For EC, the highest value recorded for raw water was in winter with 1338 µs/cm, while the lowest value was in spring with 920 µs/cm. The highest value recorded for drinking water was again in winter with 1330 µs/cm, and the lowest value was 910µs/cm in spring also. The highest value of turbidity recorded for raw water was in winter with 89 NTU and the lowest value was in spring with 26 NTU, while the highest mean value recorded for drinking water was 20 NTU again in winter and the lowest value was 1 NTU in spring. The study showed that the results of TDS values ware ranged from 622 mg/L in spring to 1024mg/L in winter. The highest value of residual chlorine was recorded in summer with 4.5 mg/L; the lowest value was zero mg/L in some of the farthest points of the plants. For sulfates, values were ranged from 289 mg/L in spring to 498.4 mg/L in winter. All the recorded values of chlorides ions for all water samples were within the allowable limits, its values ranged from 98mg/L in summer to156.3 mg/L in winter. For total hardness, the values were exceeding 500mg/L and allowable limits for Iraqi standard criteria. The highest value recorded for raw water was in winter with 520 mg/L and the lowest value was in summer with 351 mg/L, while the highest value recorded for drinking water was again in winter with 516mg/L and the lowest value was 337 mg/L in summer also. For calcium values ranged from 75 mg/L in summer to 135 mg/L in winter. The results of this study showed high levels of lead, cadmium and aluminum in most water samples which collected during study period compared with those that exceeded the acceptable limits provided from the quality control unit and which are considered to have adverse effects on health. For lead, values were found to vary from 0.0030 mg/l in summer to 0.16 mg/l in winter (higher acceptable limits is 0.01 mg/L). While in case of cadmium the values lied between 0.0014 mg/l in winter and 0.015 mg/l in summer (higher acceptable range is 0.0030). However, for aluminum these data were ranged from 0.0062 mg/l in raw water during spring to 0.29 mg/l in drinking water during spring also (higher acceptable range is 0.2). The current results showed an increased in the number of autotrophic bacteria, total coliform, fecal coliform, fecal streptococci, and E. coli during winter season in most study locations as compared to those of the other seasons for both water plants raw and drinking water while the FS values were less than those of other bacterial types for both water plants. The Total plat count results of drinking water were exceed 100 cell/ml, the allowable limit for drinking water, for some samples of both water plants. on the other hand, the TC, FC and E. coli exceeded zero cell/100ml, the allowable limits for drinking water, in many drinking water samples for both water plants

دراسة بعض تاثيرات اللقاح المحضر من العزلة المحلية لبكتيريا Klebsiella pneumoniae == Study of Some Effects of Prepared Vaccine From Local Strain of The Klebsiella Pneumoniae

اسم المؤلف: ياسر عبد الجبار عبود السوداني
اسم المشرف: عصام فاضل علوان الجمیلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم جمع خمسين عينة سريرية من قشع مرضى مصابين بذات الرئـــة. وذلك للمدة من تشرين الثاني 2013 ولغاية ايار 2013 من مستشفى ابن البلدي ومستشفى بغداد وذلك لعزل وتشخيص بكتريا Klebsiella pneumoniae التي تعد احدى العوامل المهمة المسببة لاصابات الرئة. واخضعت عينات | Fifty clinical samples collected from sputum of patients who suffered from pneumoniae in Ibn - Balady hospital and the hospital in Baghdad city during the period from November 2012 to May 2013 for the isolation and identification of Klebsiella pneumoniae, one of the important causative agents of infection occurs in the lungs. Sputum samples were subjects to the standard laboratory procedures including identification by biochemical test and VIETK system. The results showed 15 isolates were revealed as Klebsiella Spp, only 10 isolates represented K.pneumoniae, The isolates were examined to produce extracellular toxic complex (ETC) it was found that the isolate named K2 was the higher production. Two method for purification the extracellular toxic complex (ETC) were used, first Aqueous two phase systems, In this method polymer - salt aqueous two phase system was evaluated in crude extract of K. pneumoniae at varying concentration of Dextran T - 150 with 20% with polyvinyl pyrrolidone to final rate (1 : 1) (wt : wt) with 0.2M sodium sulphate. The results showed the best concentration dilution sample given as (4.25 : 0.75) with protein concentration (97.173 mg/ml) which contained ETC in the lower layer and the mice died within 4 hours, while the second method performed by using two step column chromatography, ion exchange DEAE - Cellulose and gel filtration (Sepharose - 4B). In the first step sample given lethal activity by injection to the mice after six hours with protein concentration (55mg/ml), More purification by the second step animal died after 3hours with contain protein (27.75mg/ml). Furthermore, the results of the extracellular toxic complex characterization proved that molecular weight was 39810 Dalton determined through Gel - filtration chromatography using Sepharose 6B gel. The LD50 value of purified toxin was calculated, and the result was (6.52 mg/ml) of toxin.This quantity was found effective to cause killing of 50% of the total toxin treated animals. The biological effect of purified toxin of K. pneumoniae K2 have been examined in vivo by injection of dose (0.5 ml) of purified ETC toxin that contain (10.875 mg/ml ) protein. The final part of the study involved the histopathological changes were noted, abundant mononuclear infiltrate of inflammatory cells with necrosis of lung parenchyma. The second group of mice injected with (0.05 ml of ETC) that contain protein (1.085mg/ml) represented as sub lethal dose Histopathological changes were noted showing near of the normal appearance of alveoli and alveolar space, with presence of congestion of blood vessels. The third group of mice inject with (0.5 ml from Tris - base buffer only) represented control showed normal alveoli and alveolar space with presence of bronchial. In the immunological test the sample ETC examined with ELISA and given IgG titer (189.68+50.70 ng/ml) compared with control (46.78+12.45). This titer of IgG tested with Double immune diffusion assay and gave precipitation line with antigen compared with control.

الكشف عن الرز المحور وراثيا باستخدام انواع مختلفة من التفاعل الانزيمي المتسلسل PCR == Detection of Genetically Modified Rice By Different Type of PCR

اسم المؤلف: ياسمين ابراهيم فرحان
اسم المشرف: امنة نعمة الثويني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: In recent years, foods produced by genetic engineering technology have been on the world food markets. The biosafety aspects, regulations, and labeling for these foods are still contentious issues in most countries. Thus detection and quantificationof GMOs play crucial role for developing regulations on GM foods.In this study, eighty six non - labeled rice samples from different locals and exported market were analyzed to detect the genetic modification using a DNA based detectionvmethods as, conventional Polymerase chain reaction (PCR), and Real time PCR (RTPCR).The DNA rice samples were extracted by manual C - hexadecyl - Trimethyl - Ammonium - Bromide (CTAB) method and wizard kit method. The result revealed that DNA yield by the two methods is comparable. Rice DNA tends to be of a higher concentration when purified with the CTAB method; however, this particular DNA is more easily to amplify, the optical density (OD) was recorded 1.70 - 1.98 and the concentration of DNA quantified by fluorometer DNA rice samples, ranged from 11 to 50.5 ?g/?l. The DNA rice sample has also been used successfully with the Wizard Genomic DNA Purification Kit, and showed varieties in quality, the OD was recorded 1.65 - 1.95, and the concentration between 4.7 - 43.8 ?g/?l.The rice specific gene (sps gene) was detected by PCR. The results demonstrate that the purity of the extracted DNA in all tested rice samples was sufficiently high for a sensitive PCR analysis and the primer of detected gene appeared clearly at 251pb.Three genes; CaMV 35S promoter, NOS terminator, and insecticide resistant gene Cry1Ac were used to detect of GM rice by PCR, and Real time PCR using oligonucleotide sets targeting to novel genes. The result showed that there was no positive result reaction with conventional PCR, while the outcome of gradient PCR revealed a positive reaction in one sample (Uncle Bens brown) for CaMV35S promoter only. Gradient PCR with 12 replicons for each sample was used for qualitative detection of CaMV35S promoter gene, after optimization of melting temperature and cycles run (45 cycles) , the results appeared positive in the last three grades (63.9, 64.6, 64.9) for CaMV35S promoter, but NOS terminator, and CryIAc were recorded negative results.The result of Real - Time PCR clarified that the CaMV35S promoter specific primer showed strong amplification with Ct, and Tm values were reached into 33.73, 38.63 and 61.55, 62.92 in two samples Uncle Bens brown and Himalayan brown, respectively, whereas NOS terminator gave positive results in four samples Maxims, Laasturiana, Carolin white and Mahatma, and the values Ct and Tm reached to30.87, 30.31, 30.54, 33.75 and 64.53, 64.61, 62.62, 63.87 respectively in comparison with the positive control, while CryI Ac which did not show any positive signal.It was concluded that using molecular methods like Real - time PCR will be useful tool for detecting GM rice such as a part of the approval detection processes because of the rarity of data concerning consumption of GM rice in Iraq.

تاثير الاصابة بداء المقوسات الكوندية على المستويات الهورمونية والمدورات الخلوية خلال فترة بلوغ الانسان في محافظة بغداد == The Effect of Toxoplasmosis On Hormonal And Cytokines Levels During Human Maturity In Baghdad Province

اسم المؤلف: ياسمين رياض عبد الكريم الخناق
اسم المشرف: صباح ناصر العلوجي | خولة حوري زغير
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: داء المقوسات الكونديه مرض عالمي الانتشار حيث يصاب معظم الاشخاص ذوي المناعة الجيدة بطفيلي المقوسات الكونديه وغالبا دون ظهور اعراض. ان الهدف الرئيسي لهذه الدراسه هو فهم الفروقات الجنسيه, الهرمونيه والمناعيه في سن البلوغ في الاشخاص الذين لديهم اجسام مضاده نو | Toxoplasmosis is a worldwide disease where most healthy, immunocompetent individuals infected by Toxoplasma are almost asymptomatic.The primary goal of this study is to perceive the hormonal and immunological sex - differences in puberty age who have positive anti - Toxoplasma IgG specific antibodies. The secondary goal is to inspect the endocrine - immune interaction in these persons by detecting the effect of testosterone and oestradiol hormones level on cellular immune response namely, IL - 4, IL - 12. From the first of November 2012 till the end of April 2013, 303 blood samples were collected from apparently healthy male and female students of Al - Erfan, Ignadeen and Algawahery schools and Baghdad University, Both sexes where divided into two age groups : group (A) which included subjects with age range (12 - 15) years old and group (B) which included subjects with age range (16 - 19) years old. All serum samples were tested for toxoplasmosis by using Latex agglutination test and ELISA anti - Toxoplasma IgG antibodies test. As well as, all serum samples were tested by using ELISA technique for detection of serum mean concentration of testosterone, oestradiol hormones, IL - 12 and IL - 4. The results revealed that 107/ 303 (35.31%) of the studied subjects showed seropositive toxoplasmosis, 60 males and 47 females of 107 positive samples showed high significant (p<0.01) differences in comparison to uninfected subjects. Males group B have recorded the highest percentage 34(41.46%) of the infection. Positive association was found between toxoplasmosis and testosterone level in asymptomatic toxoplasmosis cases compared to uninfected group. high mean concentration of testosterone in toxoplasmosis infected males recorded (15.03± 1.04 ng/ml) and (12.4± 0.91 ng/ml) in groups A and B respectively, in comparison to control group which recorded (8.03± 0.78) and (9.86± 0.83) in groups A and B respectively. Also toxoplasmosis infected females revealed high levels of testosterone hormone which represented (4.83± 0.06 ng/ml) and (2.55± 0.03 ng/ml) in groups A and B respectively, with a significant (p?0.05) differences between them, while the control group recorded (0.10± 0.02 ng/ml) and (0.90±0.03 ng/ml) in group A and B respectively.The present study showed a significant (P?0.05) decrease in the mean concentration of oestradiol E2 hormone in toxoplasmosis infected males and females in comparison with uninfected ones. E2 mean concentration was (41± 2.48 ng/ml) and (56± 2.91 ng/ml) for male groups A and B, respectively, in comparison to control group which recorded (67± 2.08 ng/ml) and (74± 2.42 ng/ml) in group A and B respectively, while it was (188 ± 12.48 ng/ml) and (196 ± 16.52 ng/ml) for female group A and B respectively, in comparison to control group which recorded (221±12.09 ng/ml) and (233± 15.63 ng/ml) for group A and B, respectively.The mean concentration of E2 hormone in toxoplasmosis infected females according to their menstrual cycle showed low levels in ovulation, late follicular and luteal phases, which represent (37.5 ± 2.59 ng/ml), (131 ± 16.7 ng/ml) and (76± 3.92 ng/ml) respectively, while the mean concentration of this hormone in uninfected females was (52.4± 2.88 ng/ml), (271.6± 21.04 ng/ml) and (196.2± 12.37 ng/ml) in the three phases of menstrual cycle. This study showed high significant (p?0.05) level of IL - 12 in both males and females with latent toxoplasmosis in comparison with free - toxoplasmosis groups. The mean concentration of IL - 12 in infected males was (4.75 ± 0.88 pg/ml) and (4.12 ± 0.69 pg/ml) in male groups A and B respectively, in comparison to control group which was (2.86± 0.53 pg/ml) and (2.46± 0.62 pg/ml) in groups A and B respectively, while it was (5.60 ± 0.12 pg/ml) and (6.04 ± 0.26 pg/ml) in infected female groups A and B respectively, in comparison to control group which recorded (3.32± 0.89 pg/ml) and (4.27± 0.15 pg/ml) in group A and B respectively. IL - 4 recorded quite elevated level in toxoplasmosis infected males (groups A and B) which was (15.09 ± 0.92 pg/ml) and (17.67 ± 0.78 pg/ml) respectively, in comparison to control group which recorded (13.89± 0.84 pg/ml) and (14.92± 0.69 pg/ml) in groups A and B respectively, Meanwhile the mean concentration of IL - 4 in toxoplasmosis infected females showed mild elevation in both groups A and B which was (15.14 ± 0.84 pg/ml) and (16.06 ± 1.13 pg/ml) respectively, in comparison with toxoplasmosis free subjects which recorded (16.53± 1.22 pg/ml) and (15.15 ± 0.97 pg/ml) with no significant differences between them. Interactions between the endocrine and immune systems may mediate sex differences in response to toxoplasmosis infection.

التحري عن التعبير الجيني لل FOXP3 وTGF - ?1 باستخدام الطرائق الجزيئية والمناعية في سرطان الرئة اللاصغير الخلية == Detection of FOXP3 Gene Expression And TGF - ?1 Using Molecular And Immunological Methods In Non - Small Cell Lung Carcinoma

اسم المؤلف: سهاد فيصل حاتم المقدادي
اسم المشرف: امنة نصيف جاسم | بان عباس عبد المجيد
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Studies have linked FOXP3 and TGF - ? expression to the outcome of certain cancers. FOXP3 is a marker known to be expression in T - regulatory cells while TGF - ? is a secreted protein usually detected in the extra cellular matrix.The present study aimed at focusing on the identification of immune markers namely FOXP3 and TGF - ? with their expression patterns in lung cancer patients as a useful tool to predict disease progression.Also it is aimed to design molecular evaluation of m RNA expression of both FOXP3 and TGF - ? in peripheral blood mononuclear cells and bronchial (brush) cells of patients with lung cancer and benign lesions, using qRT PCR; determining the T - reg level in the peripheral blood employing the High Rsolution Melting (HRM) as a novel method to detect Treg - specific demethylated region (TSDR); molecular DNA analysis of somatic mutation of exons 3, 6, 7 of FOXP3 in patients with lung cancer tissue and benign lesions and immunohistochemical (IHC) estimation of FOXP3 and TGF - ?1 in T - reg and cancer cells in formalin fixed paraffin embedded(FFPE)lung cancer tissue and benign lesions.Blood samples were collected from 30 patients with newly diagnosed, non small cell lung carcinoma and 30 patients with benign lesions.Patients were recruited at The Specialized Surgery Hospital and Oncology Teaching Hospital/Baghdad.Samples from 16 apparently healthy donors were used as control during the period from June 2012 to June 2013. The samples preservation with TRIzol reagent were subjected to molecular study including RNA and DNA extraction; reverse transcription; RT - PCR; HRM assay and DNA sequencing were done in the Molecular Oncology Unit/Guys and ST Thomas? s hospital/ King College/London/UK.The expression level of FOXP3 was high in 16(61.5%)in lung cancer cases.A significant difference was noticed between cancer cases from one side and benign lesions or healthy control on the other side p<0.05.Mean of FOXP3 expression(fold change)was significantly high(2.64±0.09)in cancer cases than in benign cases(1.32±0.04)and healthy control(1.38±0.06)with p<0.05.A significant association between high expression level and >60 age and squamous cell carcinoma in cancer cases P<0.05.The expression level of TGF - ? was high in 16(61.5%)in lung cancer cases.A significant difference was noticed between cancer cases from one side and benign lesions or healthy control on the other side p<0.05.Mean of TGF - ? expression (fold change) was significantly high (6.27±0.56) in cancer cases more than healthy control (2.87±0.09) with p<0.05.The association was significant between TGF - ? expression level (high and low) and age>60in cancer and benign groups (p<0.05), while no significant association with gender and cancer types were noticed.For FOXP3 mRNA expression in bronchial brush cells, the result showed no significant difference between the mean fold change of malignant(3.57 ± 0.06) and benign(4.02 ± 0.06) patients. The low expression was predominated both in cancer and benign cases. No significant differences were found between FOXP3 expression (high and low) and age; gender ; cancer types..According to FOXP3 T - reg specific demethylated region detection, results showed that the mean percentage of FOXP3demethylation in lung cancer patients (4.32 ± 0.04) was significantly higher than in benign lesions (3.22 ± 0.02) patients andhealthy controls(3.33±0.04). A positive correlation coefficient with high significant, was found in the group of cancer samples (R² = 0.6653;r = 0.69;P : 0.0017)on correlating percentage of Treg and demethylation of FOXP3 from one side with its m RNA expression on the other side.In benign lesion group was(R² = 0.5334;r= 0.59;p= 0.0027), While in the control group a positive correlation but a weak significance was found (R²= 0.2383;r=0.28;P=0.0437).FOXP3 gene sequencing revealed high frequency of missense mutations c.715 GTA>CTA : V 239 L in 17 (94.44%) in malignant sample and non cancerous cases7 (87.5%)without statistical differences. Missence mutations were also detected in exone 3 in 3(16.67%) cancer cases and in 1(12.5 %) benign lesion.No missense mutations could be detected in exon 6. Intronic mutations and silent mutation were variable in three exons without statistical differences. Many cases of adenocarcinoma have shown multiple mutations either of missense or Intronic types. Missense mutations of exon 7 were correlated significantly with an age of 60?years. Exon 3 mutations were significantly associated with adenocarcinoma. Positive FOXP3 Immunohistochemistry (IHC) staining in tumor cells was associated with high missense mutations frequency 10(55.55%) in exon7, while in exone 3 was 2(11.11%). Negative FOXP3 IHC staining in the tumor cells was associated with seven missense mutations in exon7 and one (5.55%) in exon 3, in addition 4(23.53%) cases of the exon 7 missense mutations were associated with negative FOXP3 expression in lymphocytes.The result showed that FOXP3 by using IHC staining was positive in 21(70%) of nuclei of cancer cells, and 22(73.3%)in Treg infiltrates.The positive cancer cells and Treg infiltrates associated significantly with age>60 (p<0.05).No significant association was found withgender, cancer type, while there is association with moderate differentiation compared to poorly differentiation (p<0.05). High frequence of FOXP3 expression score 3 and high intensity were appeared in nuclear cancer cells compared to benign lesions cells, while Treg infiltrates with score 1 and high intensity was high frequency in malignant and benign.The result showed that TGF - ?1 by using IHC staining was positive in 25(83.3%) in the cancer cells and 21(70%) in stromal cells. No significant difference was noted between positive expression in malignant and benign lesions p>0.05.No significant association was noticed between positive cells expression and age, gender, cancer type and differentiation p>0.05. High frequency of TGF - ?1 expression score 3 and high intensity in malignant cells and benign. Also stromal cell expression score 3 and high intensity were predominated in malignant and benign lesions. The high and moderate intensity expression was more frequent in matrix surrounding cancer cells compared to non cancerous.Total agreement and kappa coefficient between FOXP3 and TGF - ?1were poor in malignant and benign epithelial cells and stroma, while the perfect agreement was between expression of TGF - ?1in stromal cells

تعدد الاشكال الوراثي للحركيات الخلوية وHLA - DQB1 في مرضى السل الرئوي == Genetic Polymorphisms of Cytokines And HLA - DQB1 In Pulmonary Tuberculosis Patients

اسم المؤلف: خلود كريم حسن
اسم المشرف: علي حسين ادحية
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study aimed to understand the correlation between serum level of nine cytokines (IL - 1?, IL - 1RA, IL - 2, IL - 4, IL - 6, IL - 10, IL - 12, IFN - ? and TNF - ?) and their genetic polymorphisms at 16 gene positions defined by sequence specific primer - polymerase chain reaction (SSP - PCR) in pulmonary tuberculosis (PTB) patients, and in addition HLA - DQB1 gene polymorphism was also defined by SSP - PCR to determine their role in susceptibility or resistance to M. tuberculosis. Finally, serum level of cortisol was also determined in the patients.Ninety four Iraqi Arabs PTB patients (70 males and 24 females) were enrolled in the study. They were referred to the Institute of Chest and Respiratory Diseases in Baghdad for diagnosis and treatment during the period May - October 2012. A control sample of 80 apparently healthy individuals was also included and matched patients for gender (60 males and 20 females) and ethnicity. The results are summarized in the following : 1. A significant increased serum level of IL - 1? (24.16 ± 8.82 vs. 3.20 ± 1.18 pg/ml), IL - 1RA (41.31 ± 6.64 vs. 16.85 ± 5.50 pg/ml), IL - 2 (17.63 ± 3.53 vs. 7.80 ± 1.10 pg/ml), IL - 4 (9.56 ± 2.60 vs. 3.81 ± 1.70 pg/ml), IL - 10 (34.49 ± 4.60 vs. 7.61 ± 1.70 pg/ml), IL - 12 (25.16 ± 5.85 vs. 7.70 ± 1.12 pg/ml) and TNF - ? (22.52 ± 4.41 vs. 4.97 ± 1.15 pg/ml) was recorded in PTB patients compared to controls. Also, Cortisol serum level was significantly increased in patients (215.47 ± 1.33 vs. 38.63 ± 1.74 ng/ml).2. Cytokine gene polymorphism analysis revealed that neither genotypes nor alleles of IL1A - 889, IL2 - 330, IL2+166, IL4 - 590, IL4 - 33, IL6+565, IL10 - 819, IL10 - 592, IL12B - 1188 and TNF - 238 genes showed a significant variation between PTB patients and controls. In contrast, the frequency of TT genotype of IL1RN gene at position mspal 11100 showed a significant (P = 0.004) increase in PTB patients compared to controls (65.9 vs. 43.7%). For IL4 - 1098, the frequency of TT genotype was also significantly (P = 0.048) increased inpatients (82.9 vs. 70.0%). At position - 174 of IL6 gene, a significant (P = 0.002) increased frequency of GG genotype was observed in patients (55.3 vs. 31.2%). For IL10 gene, only GG genotype at position IL10 - 1082 was observed with a significant (P = 0.045) increased frequency in patients (18.1 vs. 7.5%). At position - 308 of TNF gene, a significant (P = 6.9 x 10 - 5) decreased frequency of GG genotype was observed in patients (60.6 vs. 87.5%), while GA genotype was significantly (P = 1.3 x 10 - 4) increased (38.2 vs. 12.5%). Finally, the frequency of AA genotype of IFNG gene at position +874 demonstrated a significant (P = 0.006) increase in PTB patients (55.3%) compared to controls (33.7%).3. To determine the impact of cytokine genotypes on cytokines serum level, PTB patients and controls were distributed according to their serum level in the three genotypes of each cytokine. It was found that CC genotype of IL1RNmspal 11100 in patients was observed with the highest IL - 1RA level (52.16 ± 5.81 pg/ml) compared to TT (41.39 ± 3.23 pg/ml) or TC (38.10 ± 4.54 pg/ml) genotype. The TT genotype of IL2 at position - 330 also showed the highest level of IL - 2 (22.16 ± 4.31 pg/ml) compared to TG (17.59 ± 3.40 pg/ml) or GG (13.68 ± 3.53 pg/ml) genotype in patients. The IL4 - 1098 TT genotype showed the highest level of IL - 4 in patients (10.38 ± 2.21 pg/ml) compared to TG (6.09 ± 1.20 pg/ml) or GG (3.93 ± 0.80 pg/ml) genotype. For IL10 gene, the GG genotype of IL10 - 1082 recorded the highest level of IL - 10 (40.67 ± 2.96 pg/ml), which was significantly different from AA genotype (26.66 ± 5.65 pg/ml). At position - 308 of TNF gene, serum level of TNF - ? in GG genotype of patients demonstrated a significant increased mean compared to genotype GA (24.76 ± 1.30 vs. 19.15 ± 1.12 pg/ml). At position - 238, TNF GG genotype showed a significant increase level of TNF - ? (23.02 ± 2.91 pg/ml) in patients compared to AA genotype (17.18 ± 1.53 pg/ml) of patients. Finally, IFNG+874 AA genotype was observed with the highest IFN - ? level in patients (11.07 ± 1.12 pg/ml) compared to AT (7.97 ± 1.81 pg/ml)or TT (6.10 ± 2.20 pg/ml) genotype. In contrast, no such differences were observed in controls.4. Out of the five encountered HLA - DQB1 alleles, DQB1*03 showed a significant (P = 0.005) increased frequency in PTB patients compared to controls (71.3 vs. 50.0%). It was also observed that heterozygosity at such gene locus was significantly (P = 0.03) more frequent in patients than in controls (93.6 vs. 82.5%), while homozygosity was observed with a less percentage frequency in patients compared to controls (6.4 vs. 17.5%) and the difference was also significant (P = 0.03).Accordingly, it is possible to conclude that the cytokine profile was deviated in PTB patients, and such deviation was correlated with the genotypes of some cytokines, which might also together with HLA - DQB1polymorphism confer the individual an immunogenetic predisposition to develop M. tuberculosis infection.

تقييم اختبارات PCR وطرق الزرع الاعتيادية في التشخيص المبكر لتجرثم الدم لدى الاطفال في مستشفى حماية الاطفال التعليمي في مدينة الطب / بغداد == Evaluation of PCR And Culture Methods For The Early Diagnosis of Bacteremia In Children From Welfare Teaching Hospital In Medicine City /Baghdad

اسم المؤلف: زينب صالح هادي الزبيدي
اسم المشرف: محمد ابراهيم نادر
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: استهدفت الدراسة الحالية تقييم الفحص المعتمد على تقنية PCR (polymerase chain reaction) (وطرق الزرع الاعتيادية في التشخيص المبكر لتسمم او تجرثم الدم في الاطفال.531 نماذج دم تم جمعها من الاطفال المرضى الذين اعمارهم اقل من 51 سنة ومشتبه بان لديهم اعراض تجرثم | The present study has been undertaken to evaluate polymerase chain reaction (PCR) technique in the diagnosis of bacteremia in comparison with the conventional blood culture techniques in children (infant and newborn).Blood specimens were collected from 135 children under 12 years of age suspected with fever and sepsis, obtained from Welfare Teaching Hospital/Medical City/Baghdad, for the period from April/ 2013 till January/ 2014.Blood specimens were collected and processed for Blood culture and PCR. Blood culture was performed using blood culture bottles contain brain heart infusion broth and positive results were subcultured using three media (macConkey - , chocolate - and blood agar), Gram stain, biochemical tests and conformational test (Api staph and Api 20E). Polymerase chain reaction was done using the universal primer, gram positive specific primer, gram negative specific primer, 16s rRNA primer for coagulase negative staphylococci and LacZ primer for Enterobacteriaceae.Optimization trials was carried out to increase the sensitivity of the PCR by applying 57°C in the annealing step for Gram positive specific primer and Gram negative specific primer to detect Gram positive and negative bacteria in blood respectively.Blood specimens were positive for bacteria in 69 cases (51.1%) by blood culture and 74 cases (54.8%) by PCR out of a total of 135 specimens analyzed. PCR showed more sensitive results compared to blood culture for detection of neonatal bacteremia. current results were revealed the ability of PCR to recognize five pathogens which have been negative by culture, all have been coagulase negative Staphylococci.The most frequent bacteria isolated and detected by PCR and Blood culture methods were Coagulase negative staphylococci (CoNS) (n = 60) followed by Enterobacter spp. (n = 8), E.coli (n = 5) and K.pneumoniae (n= 1). Interestingly, higher incidence rate (81.1%) were documented for the late onset sepsis (LOS) in our study compared to the early onset sepsis (EOS) (18.9%) for all bacteria. LacZ PCR efficiency have been 100% for detection of Enterobacteriaceae in blood.

تاثير انزيم الكلوكوسيل ترانسفيريز المنقى من العزلة المحلية Streptococcus mutans النمط C في انتاج الاضداد (IgY) من صفار بيض طيور الدجاج == The Effect of Glucosyltransferase Purified From Local Isolate Streptococcus Mutans (Serotype C) On Egg Yolk Antibodies (IgY) Generation In Layer Hens

اسم المؤلف: هاشم محمد زهراو الصبيحاوي
اسم المشرف: عصام فاضل علوان الجمیلي | فارس عبد الكريم
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: استهدفت الدراسة الحالية عزل وتشخيص بكتيريا Streptococcus mutans المسؤولة عن تنخر الاسنان البشري والتكلسات (plaque) واستخراج اضداد لها من صفار بيض الدجاج Yolk Immunoglobullin (IgY) لغرض استخدامها مستقبلا كمثبطات لنمو هذه البكتيريا الخطيرة ويمكن مزجها مع مع | The presented study aimed to isolate the main agent for dental caries and teeth plaque, Streptococcus mutans bacteria, and then production of specific antibodies against these harmful bacteria by the use of chicken egg yolk immunoglobulin (IgY). S. mutans had been proposed as the main etiological agents of dental caries and high levels of mutans streptococci in the plaque is correlated with a higher risk for dental caries. Seventy five plaque samples were collected from human teeth. Forty two samples were considered to be positive bacterial isolates using MS - agar (Mitist Salivares agar). Thirty five isolates were considered belonging to the group Streptococci; among these isolates 29 isolates were expected to be belonging to mutans streptococci group according to ability of producing special kind of exopolysaccharides. Ten isolates were considered as S. mutans with a percentage of 41% depending on staining with triphenyltetrazolium chloride and tolerance with NaCl 4%, 6 isolates were classified as serotype C by using Lancefield grouping identification. These isolates were tested for production of extracellular Glucosyltransferase (GTF) through determination of their enzyme specific activity. All isolates were able to produce the enzyme; Streptococci isolate (H5) identified as Streptococcus mutans serotype C was selected as the best producible isolate for GTF with a specific activity of 2.6 U/mg. It was found that GTF of the chosen isolate (H5) was produced during the middle stationary phase (18 - 35 hr.) and its maximal productivity was reached at 22 hr. Purification of S. mutans serotype (C) H5 GTF were done by ammonium sulfate, ion - exchange chromatography (DEAE - Sephacel column), and gel - filtration chromatography using Sepharose 6B column. The best percent saturation use for precipitating GTF by ammonium sulfate was 20 - 40% with specific activity 3.4 U/mg. Two purified GTF enzymes (GTF - I and GTF - II) were detected with specific activity 8.3 U/mg, 35.5 U/mg after 22.6, and 96.1 fold of purification respectively with yield 17.2%. Purification S. mutans CA - GTF (H5) were done by 8M urea, ammonium sulfate, DEAE - Sephacel column and gel - filtration (sepharose 6B) column chromatography. The purified CA - GTF was detected with specific activity 18.1 U/mg after 24.5 fold of purification with yield 20.2%. Determination of purified GTF (GTF - I, GTF - II) and CA - GTF molecular weight was done by using gel - filtration chromatography (sepharose 6B) column with presence of standards proteins. It was found that the molecular weight of GTF - I, GTF - II and CA - GTF was 125.819, 112.201 and 84.139 dalton, respectively. The ability of GTF, CA - GTF and whole cell of S. mutans to stimulate the immune system of avian hens was tested. The intramuscular rout injection of three purified antigens (GTF, CA - GTF and whole cell) in the chest of experimental hens was done. IgG from egg yolk hens (IgY) was purified through the post immunization period (9 weeks) by using polyethylene glycol (PEG) precipitation and protein content of IgY antibodies was estimated from egg yolk and serum. Each one milliliter of purified IgY egg yolk samples GTF, CA - GTF and Whole cell, protein contained 7.06, 6.97, 3.9 mg/ml, respectively while in serum protein content about2.6, 3.1 and 3.25 mg/ml, respectively. The Sodium dodecyle sulfate polyacrylamide gel electrophoresis (SDS - PAGE) of anti - GTF (IgY) indicated that purified IgY gave two bands; 47.863 and 34.673dalton which were considered to be IgY heavy and light chains respectively. the IgY - CA - GTF sample is the best in terms IgY specificity 34.07% while the two samples (GTF, Whole cell) performed 30.5% and 29.3% respectively, Igy - GTF the best in terms purity 49% followed IgY - CA - GTF 47% and IgY - whole cell 46.3%. The immunological specificity of the three IgY samples preparations was assessed by ELISA test and the best sample that produced high titration was IgY - GTF with concentration 3.5 mg/ml, followed by the IgY - CA - GTF and IgY - whole cell with concentration 3.28 and 3.1 mg/ml respectively. The IgY - GTF inhibited approximate 75% of the specific activity GTF, while IgY - CA - GTF inhibited 50% of the specific activity CA - GTF. A double immunodiffusion test for detection of the immune response between anti - GTF IgY and purified GTF, CA - GTF and Whole cell antigens were recorded. The immunological response of anti - GTF and anti - CA - GTF was indicated by the appearance of precipitation lines on the surface gel between anti - GTF and two antigens GTF and CA - GTF while in the anti - CA - GTF and anti whole cell only with homologues antigen. The effect of different concentration of inhibitor (Amoxicillin) and anti - GTF, anti - CA - GTF and anti - whole cell on the growth of S. mutans bacteria were tested using broth dilution method and diffusion method on solid medium. Anti - GTF and anti - CA - GTF had no effect on the growth of S. mutans(H5) serotype C, while anti - whole - cell and Amoxicillin were capable to inhibit the growth of bacteria at concentration 20µg/ml and 15µg/ml respectively. The minimal inhibitory concentrations in which these concentrations were noticed at 35µg/ml and 30 µg/ml respectively. The highest zone of inhibition (40 mm) was recognized with Amoxicillin at concentration of 50 µg/ml, followed by anti - whole cell with a zone of inhibition of 34 mm at concentration of 70 µg/ml.

التشخيص الجزيئي لبكتريا Streptococci الفمويه باستخدام جينات gtfs في بعض مرضى السكري المصابين بتسوس الاسنان == Molecular Identification of Oral Streptococci Using Gtfs Genes In Some Iraqi Diabetic Dental Caries Patients

اسم المؤلف: هالة كمال محسن القزاز
اسم المشرف: نورية عبد الحسين علي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت هذة الدراسة في معهد الهندسة الوراثية والتقنيات الاحيائية في جامعة بغداد خلال الفترة من تشرين الثاني 2012 الى ايار 2013، للكشف عن علاقة تسوس الاسنان بين مرضى السكري والمرضى غير المصابين بالسكري DDCP) و(NDCP اعتمادا على تسوس الاسنان من انواع Streptoco | The present study was carried out in Genetic Engineering and biotechnology Institute / University of Baghdad during the period from November, 2012 to May, 2013 to detect the relationship between diabetic and non - diabetic patients according to the dental caries occurrence and its causes by Streptococcus spp. (S. mutans, S. salivarius and S. oralis (which are isolated from oral cavity, In addition, this study was carried out to study the comparison between the traditional (bacterial culturing) and molecular diagnosis methods. The total number of the studied groups was 95 Iraqi patients (45 diabetic dental caries patients (DDCP) and 50 non - diabetic dental caries patients (NDCP) of both genders who their ages ranged from 18 - 65 years old. The patients, samples including saliva and buccal swabs that randomly collected from DDCP and NDCP who were reviewing Al - Alweyia Centers of Dental Caries and Diabetic Diseases in Al - Yarmook hospital in Baghdad city. The distribution of patients who have dental caries according to genders showed high significant differences at (p<0.01) between two genders (30 females, 15 males) for diabetic dental caries patients, while in non diabetic dental caries patients, there were significant differences at (p<0.05) between two genders (29 females, 21 males). The distribution of diabetic dental caries patients according to age showed high significant differences at (p<0.01) between two genders in age group of 36 - 50 years old, While low significant differences at (p<0.05) between two genders in age group of 20 - 35 years old and no significant differences between two genders in age group more than 50 years old, In another hand in non diabetic dental caries patients, there were no significant differences between two genders in all age groups. The results of samples (saliva and buccal swabs) culturing on mitis salivarius bacitracin agar media (MSBA) appeared that out of 95 bacterial cultures, 67 bacterial cultures were grown (32 bacterial cultures for diabetic dental caries patients and 35 bacterial cultures for non diabetic dental caries patients); S. mutans, S. salivarius, and S. oralis species were identified according to the results of microscopic examination, API kit 20 - strep, hemolysis on blood agar, motility test and catalase test. The molecular study focused on the analysis of DNA which extracted directly from saliva, buccal swabs and from the bacterial culture cells of S. mutans, S. salivares and S. oralis from both diabetic dental caries patients and non diabetic dental caries patients. Polymerase chain reaction (PCR) results revealed the presence of the product with 433, 544, and 374 bp which were related to gtfD (S. mutans), gtfK (S. salivarius) and gtfR (S. oralis) respectively in all samples (saliva, buccal swabs and bacterial culture). According to the presence of these three genes, there were high significant differences at (p<0.01) between diabetic dental caries patients and non diabetic dental caries patients, while there were no significant differences according to the percentage of presence of each gene between the three species of bacteria. Sequencing of the PCR products of the gtfs (gtfD, gtfK, and gtfR) genes region showed that nine samples gave acceptable results according to National center for Biotechnology Information (NCBI) matching, while 3 samples gave no results; this may be due to an error in sequencing system. The sequencing analysis of gtfs gene (gtfD, gtfK and gtfR) revealed that in DDCP the highest percentage of recorded mutations was in the gtfR gene.While in non diabetic dental caries patients, the highest percentage of recorded mutations in the gtfK gene than gtfR genes. In gtfR gene all mutations were substitution for diabetic dental caries patients and non diabetic dental caries patients. Nevertheless, in diabetic dental caries patients the mutations in gtfK and gtfD genes distributed between substitution and deletion mutations without recording any type of insertion mutation. But, in non diabetic dental caries patients, in gtfD all mutations were distributed between three types of mutations (substitution, insertion and deletion). The highest percentage of the effect of mutations in gtfs genes (gtfD, gtfR and gtfK) in diabetic dental caries patients were silent and missense mutation's than the frameshift mutations. on the other hand, the highest percentage of the effect of mutations in gtfs genes (gtfD, gtfR and gtfK) in non diabetic dental caries patients was missense mutations as compared with the other two types of silent and frameshift mutations

دراسة تاثير ضوء الليزر الثنائي الصمام (632 نانومتر) على بكتريا المكورات العنقودية الذهبية بوجـود المثلين الازرق كمتحسس ضوئي == Study of Photodynamic Effect of (632 nm) Laser Diode Light On Staphylococcus Aureus Using Methylene Blue As A Photosensitizer

اسم المؤلف: ضياء خليل اسماعيل
اسم المشرف: نورية عبد الحسين علي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: شملت الدراسة ثمان وسبعين عزلة من بكتريا المكورات العنقودية الذهبية عزلت من مئة وعشرين عينة تم الحصول عليها من عينات حروق وجروح قيحية وتجرثم الدم ومن مرضى يعانون من التهابات المجاري البولية, ومن اكثر من مستشفى للفترة من تشرين الثاني‏ / لغاية اذار/ 2014.تم | Seventy eight isolates of S. aureus bacteria were obtained from one hundred and twenty samples which were collected from different body sites and lesions (urine, blood and purulent wounds and burns) of patients from both sexes during the period November - 2013 to March - 2014. Methicilline sensitivity test (5µg MET disc) showed the appearance of methicillin - resistant in thirty seven Staphylococcus aureus isolates. Following exposure to laser light with a wavelength of 632 nanometer in the presence of Methylene blue at a concentration of 300µM at various exposure times (2, 4, 6, 8, 10, 12 and 15min), the results showed that the maximum decrease in viable colony counts ranging approximately from (6.9 to 3.8) log10 CFU /ml. Highly significant reduction in the viable count was achieved at 10, 12, 15 min exposure times, and 99% killing of cells were obtained when Photosensitisation of S. aureus using diode laser light at an energy density at 458.6 watt/cm2 for 15 mints. While in their exposure to the laser light in the absence of the dye or the dye in the absence of the laser light presented no significant effect on the viability of the S. aureus isolates. Both of phenotypic and genotypic investigation of the changes in virulence factors and the antibiotic - resistance were evaluated before and after irradiation with laser light.Results of photosensitization susceptibility tests showed large variations in the susceptibility, the isolates with resistant to methicillin before laser irradiation, become sensitive to it with percentage of 21.6%; in contrast the isolates with sensitive to vancomycine become resistant to it with percentage of 32.43%.On the other hand, the isolates that were resistant to Cefotaxime before laser irradiation become within the sensitivity range after laser irradiation with percentage of 51.35%, and also there were isolates within the sensitivity range before laser, become sensitive to Ciprofloxacin with percentage of 27.02% after irradiation. And the isolates of the S. aureus with resistant to Norfloxacin before laser irradiation, become sensitive to it with percentage of 16.2%. Also resulted in decrease the activity of ? - haemolysis, with 33 (90.3%) isolates of S. aureus in comparison to control as shown in blood agar method assay. In contrast had no effect on thermonuclease enzyme after irradiation.Detection of three genes represented in MRSA isolates by a confirmatory test was carried out using Polymerase chain reaction (PCR) technique. The results of the PCR amplification of mecA gene noted that it was present in 27 (72.2%) S. aureus isolates, While hlb gene detected 17(46%) isolates of 37 samples of S. aureus isolates, 14 of 17 hlb - positive S. aureus isolates(82.3%) were showed reduction in toxin production after exposure to laser light, whereas no altered or deficiency in thermonuclease gene (nuc).

علاقه بعض طرز الجين كالبين - 10 مع حدوث مرض السكري من النوع الثاني في العراق == Association of Some Calpain - 10 Gene Polymorphisms With The Incidence of Type 2 Diabetes Mellitus In Iraq

اسم المؤلف: مياسة مثنى خالد
اسم المشرف: اسماعيل عبد الرضا عبد الحسن
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت الدراسه الحاليه في معهد الهندسه الوراثيه والتقنيات الاحيائيه - جامعة بغداد خلال الفتره من كانون الثاني ولغاية حزيران - 2013 للكشف عن علاقة بعض الطرز الجين كالبين - 10 بحدوث مرض السكري من النوع الثاني في العراق. تم استخلاص الدنا من الدم الكلي باستخد | The present study was carried out in Genetic Engineering and biotechnology Institute - Baghdad University during a period from January to June, 2013, for detecting the association of some calpain - 10 gene polymorphisms with the incidence of type 2 diabetes mellitus in Iraq. Genomic DNA was isolated by using Geneaid DNA extraction kit from the whole blood; conventional PCR (SNP - 44 and Del/Ins - 19) and PCR - RFLP (SNP - 43 and SNP - 63) were used to detect the calpain10 variants by using specific primers and restriction enzymes. The study population consisted of 50 subjects with type 2 diabetes and 50 with normal fasting blood glucose (80 - 110 mg/dl). The type 2 diabetic subjects were recruited from the National Center for Diabetes treatment and Research. The non - diabetic control subjects were recruited from the same area as the comprising blood donors, healthy volunteers, or hospital /university staff members. Previous studies have detected a role for Calpain - 10 (CAPN10) polymorphisms in susceptibility to Type 2 diabetes mellitus (T2DM) in many populations. This study aimed to evaluate possible associations between these polymorphisms in the CAPN10 gene (SNP - 44, SNP - 43, Del/Ins - 19, and SNP - 63) and T2DM incidence in Iraqi population. Enrichment of allele 1(2R) in Del/Ins - 19 and 2R/2R genotype were found in T2DM patients. While the alleles and genotypes distribution of SNP - 44, SNP - 43 and SNP - 63 were not significantly different between patient groups and non - diabetic control subjects. The genotype AA in SNP - 43 and genotype TT in SNP - 63 were not found neither in T2DM nor in control subjects. of the eight haplotypes detected, enrichment of both haplotype 112 defined by variants of SNP - 43, Del/Ins - 19, and SNP - 63 and haplotype 2112 defined by variants of SNP - 44, SNP - 43, Del/Ins - 19, and SNP - 63 were seen in patients. The distribution of the other haplotypes was comparable between patients and control subjects. The calpain10 haplotype combinations were also obtained, and the haplotype combinations 111/111 and 111/112; which are created by variants of SNP - 43, del/ins - 19 and SNP - 63 and; haplotype combinations 1111/2111, 1111/2112 and 1121 / 2222; created by SNP - 44, SNP - 43, del/ins - 19 and SNP - 63; were associated with increasing the risk of T2DM.

التاثيرات المضادة للاكسدة والسمية الخلوية لمركب اللكنان المنقى من بذور نبات جوزة الطيب == Antioxidant And Cytotoxic Effects of Lignan Purified From Myristica Fragrans Seeds

اسم المؤلف: شیماء عصام عبد الوهاب البرزنجي
اسم المشرف: عصام فاضل علوان الجمیلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: In this study, natural lignan dimer was isolated from nutmeg seeds (Myristica fragrans) using organic solvent, partially purified using liquid/liquid partiation, purified using anion exchanger and chemically characterized using Benedict’s Reagent, Fehling’s Reagent and Molish’s Reagent. Then, by the aid of UPLC - PDA - IT - TOF - MS System, the molecular weight (626.221 Dalton) and the molecular formula (C39H45O7) of this dimer were determined. After that, the free radical scavenging activities were studied using stable free radical compound 1, 1 - Diphenyl - 2 - Picryl - hydrazil (DPPH). Results showed that 100, 10, 1 and 0.1 ?g/ml of purified lignan had 76.7 %, 65%, 28% and 8% scavenging activity respectively, while the same concentrations of partial purified lignan had 44.3%, 18.5%, 11% and 0% scavenging activity respectively.MTT(3 - (dimethylthiazol - 2 - yl) - 2, 5 - diphenyl tetrazolium bromide) assay was conducted to determined the IC50 for both purified and partial purified lignan using 4 different cell lines A549 (human lung adenocarcinoma epithelial cells), MCF7 (breast cancer cells), PC3(human prostate cancer cells), and HepG2(liver hepatocellular cancer cells), and to determine which cells type were be affected more by this natural lignan dimmer.The IC50 values for purified lignan were 85.17, 51.16, 108.4 and 60.21 ?g/ml while the IC50 values for partial purified one were 170.1, 84.14, 154.4 and 151.3 ?g/ml using A549, MCF - 7, PC - 3 and HePG2, respectively.The high content screening analysis (HCSA) and Cellomics Thermo Scientific maltiparametric Kits were used for the evaluation of cell - lignan interaction; 100, 50 and 25 ?g/ml of purified lignan caused 87.22, 69 and 53.36% reduction in MCF - 7cell count respectively and the same concentrations caused 98.1, 97.5 and 98.55% nuclear morphology changes. Results also revealed that these concentrations caused 7.7, 7.0 and 5.83% increase in MCF - 7 cells permeability respectively and they also caused 12.22, 11.15 and 0.2%decrease in mitochondrial membrane potential respectively, while these concentrations caused 11.12, 10.1 and 10% increase in Cytochrome C releasing from mitochondria to cytoplasim respectively.Reactive oxygen species (ROS) induction in MCF - 7 cells in the presence of 200, 100 and 50 ?g/ml of purified lignan caused 20, 11.48 and 9.61% ROS reduction respectively.MCF - 7 cell cycle was studied in the presence of 100, 50 and 25 ?g/ml of purified lignan, and results revealed that this compound blocked cell cycle at Sphase and the percentages of S - phase cells reduction were 74.33, 52.4 and 67%, respectively. This reduction was dose dependent while the same concentrations had no effect on MCF - 7 mitotic cells. Cell cycle arrest was detected immunofluorescently using BrdU antibodies (S - phase cell staining) and phosphor - Histone H3 antibodies (M - phase cells staining

التشخيس المبكر لداء السكري النوع الاول باستخدام مضاد حمض الكلوتاميك منزوع الكاربوكسيل ومضاد البيروكسيديز الدرقي == Early Detection of Type 1 Diabetes Mellitus Using Anti - Glutamic Acid Decarboxylase And Anti - Thyroid Peroxidase

اسم المؤلف: ريم محمد عبيد
اسم المشرف: منذر مصطفى فتحي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Markers have been described in type 1 diabetes mellitus (T1DM), There is a number of specific and non specific antigens have been identified. The major autoantigens involved in the destructive process of beta - cells leading to the development of type 1 diabetes are insulin hormone, glutamic acid decarboxylase (GAD), tyrosine phosphatase enzyme or Insulinoma - associated Antigen - 2(IA - 2).This study was conducted to find the relationship between antibodies for this antigens (insulin, GAD and IA - 2) and T1DM which could be used for the early detection of T1DM in normal Iraqi population. To study the importance of anti - thyroid peroxidase (anti - TPO) as a marker for autoimmune thyroid disease (ATD) in T1DM patients, and to find the relationship between T1DM and ATD.The study was carried out on 50 blood samples of men and 30 blood samples of women with age ranged from (20 - 60 years old), they were divided in to three groups : 1. Group 1 (20 men and 10 women) whom have fasting plasma glucose (FPG) above 180 mg/dL.2. Group 2 (20 men and 10 women) whom have FPG ranged from 120 - 180 mg/dL.3. Group 3 (10 men and 10 women) whom have FPG below 120 mg/dL.Blood samples were collected from all subjects, FPG and glycated haemoglobin (HbA1c) were measured. The levels of insulin, anti - insulin, anti - GAD, anti - IA - 2 and anti - TPO antibodies were measured in the serum.The statistical analysis results showed no significant difference in the presence of antibodies, HbA1c and FPG between men and women groups, inspite of some simple differences between the two groups.A significant (p<0.05) elevation in the level of FPG and HbA1c was observed in diabetic patients group compared withnon diabetic group. Significant (p<0.05) decrease in the level of insulin of T1DM patients was noticed compared to non diabetic group. Significant (p<0.05) elevation in the level of (anti - insulin, anti - TPO) in T1DM patients compared with non diabetic group. Elevation in the level of (anti - GAD, anti - IA - 2) in the T1DM patients compared with non diabetic group.The results also showed that no positive results for (anti - insulin, anti - TPO) present in the non diabetic group. One positive result for anti - GAD and one positive result for anti - IA - 2 present in the non diabetic group, which indicate the importance of anti - GAD and anti - IA - 2 antibodies ssay in normal population that could be used as early detection of T1DM.

دراسة تشريحية مقارنة لبعض الانواع البرية من ذوات الفلقتين النامية في محافظة بغداد == Comparative Anatomy of Some Wild Dicots Spp. Grown In Baghdad Province

اسم المؤلف: زبيدة عبد اللطيف اسماعيل
اسم المشرف: علي حسين الموسوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present investigation dealt with comparative anatomical characters of 29 selected wild species that belongs to different families growing in different parts of Baghdad Province. These species are : 1. Brassica deflexa Boiss.2. Brassica rapa L.3. Cardaria draba (L.) Desv.4. Sinapis arvensis L.5. Sisymbrium irio L.6. Strigosella africana (L.) Boch.7. Stellaria media (L.)Vill.8. Atriplex nitens Schkuhr.9. Chenopodium album L.10. Chrysanthemum coronarium L.11. Lactuca serriola L.12. Sonchus oleraceus L.13. Medicago polymorpha L.14. Melilotus indicus (L.) ALL.15. Vicia sativa L.16. Erodium cicutarium(L.) L'H17. Geranium rotundifolium L.18. Lamium amplexicaule L.19. Malva parviflora L.20. Plantago lanceolata L.21. Polygonum aviculare L.22. Rumex dentatus L.23. Ranunculus muricatus L.II24. Veronica polita Fries.25. Astrodaucus leptocarpus (Hoghst.) H. Riedl26. Urtica urens L.27. Verbena officinalis L.28. Lippia nodiflora (L.) Rich.29. Tribulus terrestris L.Some of these species have been anatomically investigated for the first time.Anatomical characters might be useful for plant biologist for the identification of important wild plants as an additional character at global level. Comparative anatomical characters were used; such as characteristics of ordinary epidermal cells of stems and leaves and stomatal complexes. The epidermis possesses number of important diagnostic character that offer valuable clues for identification, like size, shape, in addition to indumentum of both stems and leaves. Venation system was investigated and compared. Cross sections of root were useful taxonomically especially the thickness of epidermis, cortex, phloem and xylem. The study observed presence of sclerenchyma tissues in the root cortex of some species. Cross sectioning of stems and petioles were also studied. Epidermis, cortex, pericycle, vascular bundle shapes and numbers, pith characters, presence of crystals and tannin filled cells were useful aid in distinguishing species. Vertical sections of leaf blades, shape and number of vascular bundles, thickness of palisade and spongy layers and other mesophyll characters were important taxonomically.ccording to some of these characters, species were divided into groups. This study concluded the presence of variations in characters and these were presented for the first time comparatively, so that, the anatomical characters were a good support to the exomorphological characters in the studied species. Field photographs of the different studied plants and sections of organs were put in addition to numerous tables in this work. The above results were discussed scientifically regarding mainly the environmental factors.

فعالية مستخلصات بعض الطحالب الكبيرة للفطريات الممرضة للنبات == Activity of Some Macro - Algae Extracts Against Phytopathogenic Fungi

اسم المؤلف: دنيا يوسف محمد يوسف
اسم المشرف: عبد اللطيف محمد جواد
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية عزل وتشخيص طحالب كبيرة من بيئتين مائيتين محليتين مختلفتين. تم عزل ثلاث طحالب كبيرة هي Cladophora glomerata (N) وEnteromorpha rafsii من منطقه بحر النجف في محافظة النجف, في حين عزل Cladophora glomerata (R) من احد مبازل منطقة الراشدية ش | The present study includes isolation and identification of Macro - algae from two different environmental water bodies. Three Macro - algae were isolated, Cladophora glomerata (N) and Enteromorpha ralfsii from Baher Al - Najaf region in Holy Najaf city. In addition, C. glomerata (R) was isolated from Al - Rashdiya, north of Baghdad.Phytopathogenic fungi from soil and some infected fruits were also isolated. They were identified as Pythium ultimum and Rhizoctonia solani.Water and ethanol were used to extract each algae to evaluate their antifungal activity against isolated phytopathogenic fungi. Different concentrations of these algal extracts had been prepared which are (10, 25 and 50) mg/ml and the antagonistic activity against the isolated phytopathogenic fungi were evaluated, the hot and cold water extracts did not show any antagonistic activity against fungi. However, all these extracts were ignored in the future work for this study. Furthermore, the hot ethanolic extract was more efficient than these of cold ethanolic extracts. Results have been shown that there were significant differences when macro - algal ethanolic extract were used.C. glomerata (R), which isolated from Al - Rashdiya, was more efficient against tested fungi than the same alga what isolated from Baher Al - Najaf region. However, E. ralfsii extract was more efficient against tested fungi than C. glomerata (N). Percentage of inhibition against P. ultimum when hot ethanolic extract of E. ralfsii, C. glomerata(R) and C. glomerata(N) were (88.8, 83.3 and 63.3) respectively.However, results for cold ethanolic extract of the same algae were (63.3, 52.2 and 32.2) respectively when 50 mg/ml of algal extracts were used.Percentage of inhibition against R. solani when hot ethanolic extract of E. ralfsii, C.glomerata (R) and C.glomerata(N) were (94.4, 100 and 78.8) respectively.However, results for cold ethanolic extract of the same algae were (66.6, 64.4 and 48.8) respectively when 50 mg/ml was used. According to the difference in biological activities against those phytopathogenic fungi caused by the same species of macro - algae or different genera from different environments. Due to difference in biological activity of the same macro - algae species Physiochemical parameter for each environments obtained such as air and water temperature, Turbidity (NTU), Electrical Conductivity(EC)and Salinity%, Water pH, CA+2 concentration, Mg+2 concentration, Total Hardness and Total Alkalinity. Results indicated that soaked Cucumis sativus and Capsicum annum seeds in cold and hot ethanolic macro - algae extracts of (E. ralfsii, C. glomerata (R) and C.glomerata (N) for 24 hours were able to protect seed germination that have been grown in a Petri dish contaminated with P. ultimum and R. solani comparing with control. In addition, results indicated that spraying C. sativus and C. annum seedling with these algal extracts within two weeks old again protects these seedlings from the same phytopathogenic fungi either before or after 24 hr. However, results indicated that adding the algal extracts within two months age to the C. sativus and C. annum plants again protects these plants from the same phytopathogenic fungi comparing with control and fungicide treatment. Moreover, results indicated that the active chemical compounds in E. ralfsii was Tannins, Saponins and Flavonoids. While C. glomerata was contained Tannins, Saponins, Alkaloid and Phenols. Results indicated that both hot crud methanolic extract of C. glomerata (R) and E. ralfsii have a lot of active chemical compounds against micro - organisms by using the GC - Mass Spectrometry technology.

التحري عن بعض عوامل الضراوة للزائفة الزنجارية المعزولة من الحليب الخام والجبن الطري == Detection of Some Virulence Factors of Pseudomonas Aeruginosa Isolated From Raw Milk And Soft Cheese

اسم المؤلف: عبد الكریم كاظم عبد الحسین الحسب
اسم المشرف: علي حسن احمد الشمري
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: صحة الغذاء
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: In order to detection of Pseudomonas aeruginosa in food - chain especially from Cows raw milk and soft cheese with its whey, this study was designed in some regions of Baghdad (College of Veterinary Medicine, Abu - Ghraib, Al - Sadrya & Al - Radwaniyah), in which a standard isolation methods were used with some modification processing by new, modern and rapid technology tools such as chromogenic medium CNP agar and Electronic rapid Microbact TM 24E panel identification system supported by standard color differential chart and online American Type Culture Collection (ATCC) Codes; aids in rapid and precise identification, differentiation, confirmation and enumeration of P. aeruginosa isolates from raw dairy samples; as well as, detection of biofilm producing versatile morphotypes with chameleon phenomenon and fruity odour; and demonstration the activity of food spoilage enzymes (protease, lipase & lecithinase) of isolates with thermo - stability and viability in different temperatures with bitty cream and ropy fermentation phenomenon. The results profile showed : 1. Isolation of 46 (76.7%) isolate of P. aeruginosa from a total of 60 Cow Dairy samples : 30 Raw Milk samples (pooled from milk cans and apparently healthy Cows with a history of mastitis cases) as 19 (31.7%) isolates from regions of College of Veterinary Medicine, Abu - Ghraib and Al - Sadrya (10 from each region); and 30 Soft Cheese with its Whey samples (pooled from unhygienic processed cheese and containers in unclean dirty environment and apparently carrier or diseased workers) as 27 (45%) isolates from regions of Abu - Ghraib, Al - Sadrya and Al - Radwaniyah (10 from each region). Isolation percentages of oxidase positive P. aeruginosa from selected Regions in Baghdad Province indicates significant differences among regions at a level (P?0.05), in which highest isolation percent of P. aeruginosa from Abu - Ghraib as 20 (33.4%) isolates (10 isolates from raw milk and 10 isolates from soft cheese) in accordance to 17 (28.4%) isolates from Al - Sadrya (9 isolates from raw milk and 8 isolates from soft cheese), 9 (15%)SUMMARYisolates from Al - Radwaniyah (9 isolates from soft cheese) without any isolate from College of Veterinary Medicine, which may indicate good hygienic measurements.2. Segregation of isolates into two Haemolytic Patterns : Livestock type origin, lysis only sheep blood (L - type) as 19 (41.30%) isolates from raw milk samples; and Zoonotic type lysis, both blood (Z - type) as 27 (58.70%) isolates from soft cheese with its whey as high prevalence due to critical processing chain, risky carrier individuals and contaminated environment or polluted water supply. This may indicate indirectly genetic diversity and host or cross individual tropism in haemolysins - phospholipases activity according to different types of samples, regions and hosts.3. The study revealed isolation and segregation of versatile morphotypes and five pigments with fruity odor (Chameleon phenomenon) with mucoid drippy due to alginate or small colonial variants smooth or rough or both especially in isolate code M7 from mastitic milk and isolate code C27 from contaminated soft cheese and its whey. Wrinkled - Corroded green morphotypes were more evident in blood agar due to secretion of siderophore pyoverdine in order to chelating iron for activation of isolate. Motility Pattern with TTC salts was evident in all isolates with three types of motility : swarming, swimming and twitching, that noticed obviously in M7 isolate.4. New technology biochemical identification panel system (Microbact TM 24E) confirm that isolates were P. aeruginosa at level (P?0.01) after online matching with standard Remel ATCC Octalcodes bank of P. aeruginosa, in which 5 octalcodes typical and atypical were documented, that indicate genetic diversity in biochemical reactions of isolates and their morphotypes, modified microbial gene sharing protocol of P. aeruginosa with other bacteria and highly Sensitivity and Specificity of the test panel.SUMMARY5. Detection and isolation of Biofilm producing isolates by five methods, in which microtiter plate assay was the most sensitive and realizable technique while Congo red agar test detect quantity and type of biofilm production through colour of colonies (black very strong and huge, gray strong, pink moderate and white fair or non - producer). Biofilm produced in most morphotypes with pellicles at liquid interphase and ropy viscous threads (sliminess) and bitty cream especially in isolate codes M7 and C27. Small colonial variants of some morphotypes can revert to mucoid drippy variants. M7 showed unusual ropy thread of 30 cm long.6. The results showed secretion of thermo and psychro stable and labile food spoilage enzymes (protease, lipase and lecithinase) from isolates, in which versatile morphotypes showed indirectly diverse genetic ability, this may indicate variation of thermal tolerance mechanisms (heat shock proteins and biofilms formation) in isolates in accordance to time and temperature of processing, that linked genetically with the quorum sense mechanisms of acyl homoserine lactone gene regulatory region (AHL) in P. aeruginosa isolates (Stress Hardening phenomenon).7. The results showed the genetic ability (indirectly) of some isolates to resist, tolerate and proliferate in acidic environment at pH 2 & 4 especially isolate M7 & C27, while intermediate to susceptible tolerance of pH were noticed in others. This may indicate the power of electromagnetic charged net field of complex polymeric matrix of alginate exopolysaccharide layers in protecting some isolates from adverse acidic environment due to quorum sensing behavior of M7 & C27.Therefore, it could be concluded from this study that P. aeruginosa was prevalent in food chain especially from Cows raw milk and soft cheese with its whey samples from some regions in Baghdad, and Biofilm producing isolates with their thermo and psychro stable enzymes complex system and their tolerance to acidic environment stressors were more dangerous thus, we recommend monitoring these products periodically for insurance of public health.

احتمالية حدوث العيوب في عاج الجذر نتيجة لاستخدام انظمة مختلفة من مبارد النيكل تيتانيوم == The Incidence of dentinal root defects caused by different Nickel Titanium instruments

اسم المؤلف: نور الدين علي سعيد
اسم المشرف: ايمان محمد الزقة
الموضوع العام: طب الاسنان
السنة: 2014
الموضوع الدقيق: معالجة الاسنان
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يعد الاعداد الميكانيكي الاحيائي لقنوات الجذر احد الخطوات الرئيسية لتحقيق حشوة جذر ناجحة بسبب امكانية ازالة الاجسام البكتيرية والحطام المتراكم وتسهيل وضع حشوة الجذر داخل القنوات. الغرض من هذه الدراسة هو لملاحظة ومقارنة احتمالية حدوث العيوب في عاج الجذر نتي | Vertical root fracture and crack formation can be seen in root dentin during and after endodontic procedures. Vertical root fracture is one of the most frustrating complications of root canal treatment, which often results in tooth extraction. The aim of this in vitro study was to compare the effect of different Nickel Titanium instruments (ProTaper Universal, EndoSequence, ProTaper Next and RECIPROC) on the dentinal wall in term of dentinal defects.Seventy - five straight palatal roots of freshly extracted human maxillary first molars teeth were used in this study, the palatal roots were sectioned at 11 mm from the apex and wrapped with two layers of aluminum foil, then placed in the center of elastic silicon mold. Fifteen roots were left unprepared to serve as a negative control group, the remaining 60 roots were divided into 4 groups, each group contained 15 roots : • Group I was prepared by rotary ProTaper Universal system.• Group II was prepared by rotary EndoSequence system.• Group III was prepared by rotary ProTaper Next system.• Group IV was prepared by reciprocating RECIPROC system.All canals were prepared to MAF # 40. After each file size of the (rotary files) or after three pecking motion of the (reciprocating files), the file was removed from the canal and the canal was irrigated with 1 ml of 2% of sodium hypochlorite. All files were cleaned periodically to prevent clogging of flutes during instrumentation. The canal remained patent by insertion size 15 K - file.When the instrumentation was completed, the roots were then embedded in a clear acrylic resin blocks and each root was sectioned into three levels : apical, middle and coronal. All samples were examined from coronal direction under Stereomicroscope. The number and the type of dentinal defects were recorded and classified. The collected data was tabulated and subjected to statistical analysis using chi - square test.The EndoSequence group showed the lowest number of dentinal defects followed by ProTaper Next group and then ProTaper Universal group. The highest number of dentinal defects were observed in RECIPROC group. Chi - square test showed that there were highly significant differences among groups (P < 0.01). The results showed a non - significant difference between EndoSequence and Protaper Next group (p > 0.05), and a significant difference with ProTaper Universal group (P < 0.05) and a highly significant difference with RECIPROC group (P < 0.01). The ProTaper Next group showed a significant difference with ProTaper Universal group and a highly significant difference with RECIPROC groups. There was no significant difference between RECIPROC and ProTaper Universal groups.

انسلاخ المبيضات اللعابي المقاس بواسطة تفاعل البوليميراز المتسلسل الكمي اللحظي كاداة تنبؤ لحصول التهاب المبيضات الفموي عند مرضى الربو المتعاطين لدواء بيكلوميتازون المستنشق == Salivary Candidal Shed Measured By Real Time Quantitative Polymerase Chain Reaction As A Predictor of Oral Candidiasis In Asthmatic Patients On Inhaled Beclomethasone Dp Therapy

اسم المؤلف: نغم قطران رحيم
اسم المشرف: رعد محي الدين حلمي
الموضوع العام: طب الاسنان
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: الربو القصبي المزمن يمثل مشكله صحيه جديه في كل العالم وهو عادة يبدا في عمر الطفوله ويبقى المريض في كثير من الاحيان يتعاطى العلاجات المستنشقه مدى الحياة.انتشار الربو القصبي يزداد في كثير من دول العالم مما يجعله احد الامراض المعيقه والمهدده للحياة باعتبا | Asthma is a serious global health problem that usually starts in childhood and continues along patient’s life and most of them have to take inhaled therapy lifelong. The prevalence of asthma is rising in many countries of the world, making asthma one of the most disabling and life threatening diseases effecting patients with different age groups and social classes with variable clinical spectrums and outcomes starting from mild to severe disabling syndrome rendering nonproductive individuals to severe life threatening and sometimes fatal disease. Those patients continue inhaled steroid therapy for long duration, considered by GINA as first line treatment , however such a treatment is associated with local adverse effects on oral tissues, and one of the main concerns is the development of oral candidiasis, a chronic, potentially invasive, infection of oral tissues with fungus candida species resulting in failure of treatment. The scientific interests about such a serious complication have been raised over the past years and debates continued as the candida is considered one of the normal flora of the oral cavity in most of healthy population, however oral candidiasis in pathogenic forms still occur in immunocompromised patients including asthmatics on inhaled corticosteroid therapy.Aim of the study1. To investigate the effect of inhaled corticosteroid therapy in form of Beclomethasone Dipropionate250 microgram/dose CFC free in asthmatic patients on salivary candidal shed estimating by Real Time quantitative Polymerase Chain Reaction (RT qPCR) applied on direct unstimulated saliva samples.2. Evaluation of this shed as prediction of occurrence of oral candidiasis. Patients, Materials and MethodsThis study was conducted at Al Zahraa consultational Asthma and Allergy center - Baghdad Al Karkh Health Directorate from September 2013 to February 2014.The included patients were divided into 3 groups. Group A included asthmatic patients on inhaled Beclomethasone therapy, group B included asthmatic patients that never used any form of inhaled steroid therapy and group C was those asthmatic patients on inhaled Beclomethasone therapy who developed oral candidiasis. All patients were receiving two daily doses. The same exclusion forma was applied to choose Group D who were control healthy individuals. Each included patients and control cases were instructed to give saliva samples in the standard way and samples taken for estimation of candidal count by real time quantitative Polymerase Chain Reaction.Results During the study period, 698 patients were examined, however only 63 patients were included in the study. Those 63 were classified as follows : 28 patients were found on group, 28 were found on group B and only 7 patients were found on group C and 20 cases were selected in group D as a control.Group C patients were having the highest salivary candidal shed (mean=58.7x106 cn/ml) and longest duration of asthma (Mean=2718 days) and longest duration of Beclomethasone therapy (Mean=2224 days) and this was significantly higher than other groups (p<0.05).Group A patients were having the second highest rank after group C in regard of salivary candidal shed and both duration of asthma and Beclomethasone therapy. Inside each group, duration of asthma and duration of Beclomethasone therapy were associated with moderate positive correlation with salivary candidal shed (R>0.5).Age difference between groups were insignificant and age factor had no correlation with salivary candidal shed inside the groups.Discussion : Salivary candidal shed over the groups was directly related to the duration of inhaled Beclomethasone therapy and duration of asthma. They are significantly higher (p<0.01) as we go further from each group to the next, both appear as the reason behind increased candidal shed in groups A and C.The salivary candidal shed curve will gave us a very useful tool for follow up those patients on inhaled steroid therapy ,In addition , predict the risk of developing oral candidiasis and when to interfere prior to the establishment of Oral Candidiasis state as that we have noticed in the group C, in which it was recommended to stop inhaled steroid therapy and the patients would lose the benefits of the therapy.Conclusions1. Resting saliva samples with realtime quantitative PCR was a rapid and accurate tool in diagnosis of oral candidiasis.2. Salivary Candidal Shed could be used as a tool in predicting oral candidiasis in asthmatic patients on inhaled Beclomethasone therapy.3.Longer duration of asthma and longer duration of Beclomethasone therapy were associated with increased salivary candidal shed and established oral candidiasis have the highest amount of shed.4. Candida albicans are present in the oral cavity of any individual of our population

تقيم التسريب الجزني لمركبات الراتنج للحشوات الخلفية سونك فل مركب مستند على السيلورين ومركب نانوفل مستند على الميتاكرليت في : دراسة مقارنة == Microleakage Evaluation of Sonicfill

اسم المؤلف: مهند احمد الحداد
اسم المشرف: حكمت عبد الرحيم الغراوي
الموضوع العام: طب الاسنان
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Resin composites have improved greatly since their introduction and are now the materials of choice for most of the restorations. This study was conducted with aim of comparing in vitro the marginal adaptation of three different, low shrink, direct posterior composites Sonic fill™ (nanohybrid composite), Filtek™ P90 (Silorane - based composite) and Filtek™ Z350 (nanofill composite) of a standardized Class V cavity after thermoal cycling. Sixty sound, freshly extracted human premolars were prepared with standardized Class V cavities. The specimens were divided into three groups of twenty teeth each according to the material used : Group A : The teeth were restored with Sonic fill™ posterior composite unidose capsules, Group B : The teeth were restored with a silorane - based posterior composite (FiltekTM P90), Group C : The teeth were restored with nanofill methacrylate - based composite (Filtek™ Z350 XT).After specimens were stored in distilled water at 37°C for 7 days, all specimens were subjected to thermocycling at (5° to 55 °C), then immersed in 2% methylene blue dye for 24 hours. For each group microleakage at the occlusal and gingival regions was estimated by determining dye penetration using scoring system under steromicroscope (10X). The statistical analysis of the results showed that, Filtek™ P90 silorane based composite exhibited the lowest mean of microleakage, with statistically highly significant difference (P < 0.01) when compared with Sonicfill™ composite and Filtek™ Z350 XT nanofill composite. On the other hand, the results of this study showed that Sonic fill™ composite exhibited no significant difference (P > 0.05) as compared with Filtek™ Z350 XT nanofill composite. Also, the results in this study showed that there is a highly significant difference (P < 0.01) in dye penetration between occlusal and gingival regions for each group

ضبط الحواف ومقاومة الكسر لاغلفة ال Ceramill Sintron المعدنية المصممة والمصنعة بالحاسوب مكسوة بثلاث انواع مختلفة من الخزف == Marginal Discrepancy And Fracture Resistance of CAD\CAM Ceramill Sintron Metal Copings With Different Porcelain Materials

اسم المؤلف: مصطفى نبيل عبد الغني
اسم المشرف: عمار عطا الله علي السعدي
الموضوع العام: طب الاسنان
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: دقة الحواف والقوة، لاي ترميم معوض للاسنان هي صفة مهمة. ويمكن اعتبارعدم تطابق للحواف او قلة القوة سبب مهم يؤدي الى فشل التعويضات الاصطناعية للاسنان.لذلك تم اجراء هذا البحث لغرض تقييم اختلاف ظبط حافات ومقاومة الكسر لتيجان السيراميك المصنعة من اغلفة الC | Marginal fit and strength are important criteria for any restoration; poor marginal fit and low strength may be considered as a source of prosthesis failure. This study was performed to compare the marginal fit changes and facture resistance of metal ceramic crowns constructed from Ceramill Sintron metal coping veneered with three different porcelain veneering materials (Vita Master Koromikos VMK, Willi Geller Creation CC and GC initial MC). Also evaluating the influence of thermocycling on load at fracture value. The method included the use of master brass die which was milled to represent the prepared maxillary central incisor. Sixty metal copings were milled directly using Ceramill Sintron blank, then divided randomly into three groups. Each group consist of twenty metal copings, then veneered with porcelain : VITA for group A, Creation for group B and GC for group C. The marginal gap was measured before and after porcelain veneering for each group using travelling light microscope, then marginal fit changes was calculated. Fracture resistance test was done by Instron®, the master die was duplicated to sixty analogs then each analog was fixed into acrylic base. After that each crown was cemented onto the corresponding die analog using zinc phosphate cement. Ten samples of each group were subjected to compressive loading to fracture and other ten subjected to thermocycling followed by compressive loading to fracture.The result showed that GC group was highly significant different from other tested groups in marginal fit changes, whereas there was no significant different between VITA and Creation group. While there was significant difference among tested group at (P < 0.01) in fracture resistance, and the effect of thermocycling process did not significantly reduce the load at fracture for all groups.This study concluded that GC group present the least marginal fit changes, while the fracture resistance was highest for VITA group follow by Creation then GC. Thermocycling reduce load at fracture but not significantly.
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