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عزل وتشخيص الفطريات المصاحبة لاخماج الجيوب الانفية وحساسيتها لبعض المضادات الفطرية == Isolation And Identification of Sinusitis Infections Borne Fungi And Its’ Sensivity To Antifungals

اسم المؤلف: رواق حيدر عبد النبي المحنة
اسم المشرف: ماجد كاظم عبود الشبلي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: جمعت ((97 مسحة انفية من المرضى المصابين باخماج الجيوب الانفية, ولكلا الجنسين وباعمار مختلفة والذين راجعوا العيادات الخاصة بالانف والاذن والحنجرة Ear Nasal Throat في مدينة الديوانية للفترة من تشرين الثاني 2012 الى نيسان 2013, لغرض عزل وتشخيص الفطريات المسب | A total of 97 nasal swab were collected from patients with sinusitis, for both sexes with different ages who attended private clinics of Ear Nasal Throat in the city of AL - Diwaniya, for the period from November 2012to April 2013, for the purpose of isolating and diagnosing fungi that cause inflammation of the sinuses and test their sensitivity toward some antifungal as well as the study of the effect of some factors on the infictions rats, which include age, gender, housing, and professions and relationship of chronic diseases. Specimen were cultivated on culture media SDA to determine the types of fungi responsible the infection, it is found that the samples gave positive growth were about 36 at a rate of 37.11 %, while the samples which gave a negative growth were about 61 at a rate of 62.88 % Note that some samples give more than one fungal type so amounted to 44 the number of fungal infections and 45.36 %.The isolated fungi included and their rates Aspergillus flavus13.63%, Aspergillus niger 13.63 % , Aspergillus terreus 4.54 % , Pencillium sp 18.18 % , Blastomyces dermatitidis and Cladosporium sp 11.36 % of each one , Alternaria sp and Trichophyton sp 4.54 % of eachone , Troulopis sp , Mucor sp , Rhizopus sp, Paecilomyces sp, Rhodotorula sp, Ulocladium sp, Geotrichium sp and Bipolaris sp 2.27% of each one. The presnt study showed that females cases are more than males cases, where the percentage of female infection about 63.63% but male infection rate of about 36.36%, patients ranged in age between 11 - 76 years and found that the infection was more frequent among age groups 20 - 29 years 29.54% and less frequent among age groups 19 - 11 years 9.09 %. About the injury season shows that increase in the cold months of the year and less in the warm months, as it recorded the highest rate of infection during November at a rate of 43.18 %, while there have been no injury during April, as for Relationship of injury with housing areas of patients has been shown that the highest proportion of the injury people registered from urban areas 52.27 % compared to residents of rural areas 47. 72 %. The role of the professions in the incidence of injury has been shown that the highest recorded injury among housewives40.9%and the least among the unemployed 2.27%, while the relationship of chronic diseases has revealed the presence of 15 people suffering from chronic diseases and fungal infections arate of 15.45 %, the more chronic injuries were pressure disease, where they formed an injury rate of 40%.Results showed the inhibitory effect for antifungal Itraconazole, Ketoconazole, Econazole, Clotrimazole, Nystatin, Amphotericin B against 12 type of fungus Pencillium, Blastomyces dermatitidis, Aspergillus flavus, Aspergillus niger, Aspergillus terreus, Cladosporium sp, Alternaria sp, Troulopsis sp, Mucor sp, Ulocladium sp, Geotrichum sp , Bipolaris sp , the antifungal Econazole was the most antifungal effective against isolated fungi and diameters of inhibition rate 30, 38, 20, 33, 30, 40, 35, 39, 27, 31, 19, 44? m of fungal species respectively, while the antifungal Amphotericin B show less effect against isolated fungi at arate of inhibition diameters 14, 19, 10, 10, 8, 12, 26, 18, 17, 22, 18, 21? m, respectively.As for the values of the MIC and MFC for Ketoconazole and Itraconazole for the fungi Aspergillus niger, Aspergillus flavus , Geotrichum sp found that antifungal Ketoconazole owned less MIC against all fungi, as it amounted to 0.24 Mg /ml of each one, while the MF Cagainst Aspergillus niger and Geotrichum sp was 7.8Mg /ml against Aspergillus flavus was 0.48 Mg /ml, while the antifungal - Itraconazole owned less MIC was 31.25 Mg /ml against all fungi, while the MFC was 62.5 Mg /ml against all isolates.The presnt study showed That fungal infections is one of the important causes of sinusitis infections and fungus Aspergillus spp is the most common.

دراسة مقارنة لعزلات بكتريا Staphylococcus aureus المعزولة من مياه النهر ومن حالات سريرية == A Comparative Study of Staphylosossus Aureus Bacteria Isolated From River Water And From Clinical Cases

اسم المؤلف: بنين مدلول امانة الزيادي
اسم المشرف: علي عبد رحيم الناشي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: شملت الدراسة جمع 120 عينة اخذت من مصدرين مختلفين, 60 عينة منها جمعت من بيئة مائية هي نهر الديوانية, بينما كانت 60 عينة سريرية جمعت من الاشخاص الراقدين والمراجعين لمستشفى الديوانية ومن الاصابات الجلدية المختلفة التي شملت الدمامل, الحصف, التقشر الجلدي, الخر | The study included assemble 120 samples which had been taken from two different sources, 60 samples collected from An aqueous environment which is Al diwaniya river, where the rest 60 samples have been collected from those people who were admitted to the Al diwaniya hospital and from of different ages from male and female. Where the environment examples collected from different places of riverbed Al diwaniya. The duration of collection samples has lasted for 3 months, started One handrad isolated had been diagnosed that proved its belong to a bacteria Staphylococcus aureus, 50 isolated where taken from environment isolate and the other 50 taken from clinical isolate depending on the diagnostic methods which included tests of farm, microscopic and biochemical, It was also confirm the diagnosis of bacterial using accurate diagnosis that uses device VITEK System tested sensitivity of bacteria isolate of S. aureus, against 12 selected antibiotic, environment and clinical cases has shown resistance and sensitivity of different varied by source isolate, the nature of the antibiotic and the resistance and sensitivity have been determined by measuring diameters of areas inhibition around tablets of antibiotics it used.All the clinical isolates showed a complete resistance for the antibiotic Lincomycin with 100% percentage, where it's resisted Pnicillin G with 92%, Nalidixic acid with 42%, while the highest resistance of environment isolates was 97% for Lincomycin. The clinical isolates showed a sensitively against the two antibiotics which are Cefamandol and Cephalothin, where their percentages reached 98% and 89%, respectively. Where the percentage of environment isolates reached for the same antibiotics 100% and 97% respectively. In general, the clinical isolates were more resisted for the antibiotics than environment isolates in overall rate.differed values of minimum inhibitory concentrationsmic MIC and the minimum bactericidal concentration MBC among each other with variation of clinical isolates from side and differed with environment isolates from another side while the values differed with environment and clinical isolates from third side, where the highest value reached of MIC against Polymyxin B is (20 - 120) and (100 - 240) mg/ml for environment and clinical isolates respectively, while the highest value of MIC that also was against Polymyxin B reached (40 - 140) and (130 - 250) mg/ml for environment and clinical isolates respectively. Where the value of MIC against Cephalothin reached (0.01 - 0.6) and (0.8 - 2.0) mg/ml for environment and clinical isolates respectively. Finally, the highest value of MBC also was against Cephalothin reached (0.08 - 0.9) and (0.9 - 3.0) mg/ml respectively. In general, the bactericidal and inhibitory concentrations of the clinical isolates are higher concentrations from those bactericidal and inhibitory concentrations for environment isolates.I studied the virulence factors that come from strains of S. aureus bacteria environment and clinical which included Capsula, enzymes of Coagulase that are associated and free, , Lipase, Hyaloronidase and B - Lactamase in addition to Haemolysin clinical isolates and Leucocidin and all the environment isolates are the highest percenage in the ability to form and possess virulence factors compared with environment isolates, where the highest percentage to produce virulence factors is belongs to the Haemolysin which reached 94% percentage in the clinical isolates where in the environment isolates reached 64% percentage followed by Leucocidin that reached 84% percentage in the clinical samples and 40% percentage in the In environmental samples.Also the percentage of blood Coagulation enzyme the associated and free is the highest percentage in the clinical isolates which reached percentage 42% and 38% for the associated and free respectively, where the percentage in environment reached 20% and 36% respectively and the virulence factors was the least presence in the environmental and clinical isolates is the portfolio that reached percentage of presence 22% and 12% respectively.

استعمال بعض انواع الطحالب والنباتات المائية في المعالجة الحيوية لمياه محطات المعالجة في مدينة الديوانية - العراق == Using of Some Dominant Algae And Aquatic Plants In Bioremediation of Waste Water From Wastewater Plants In Al - Dewaniya City, Iraq

اسم المؤلف: رائد كاظم عبد الاسدي
اسم المشرف: فؤاد منحر علكم | حسين يوسف الركابي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة المائية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية على نوعين من المياه العادمة الصناعية المطروحة من مصنع نسيج الديوانية والمياه العادمة البلدية من محطة معالجة الديوانية، وللمدة من ربيع 2011 الى ربيع 2012 وهدفت الى اجراء مراقبة فصلية لنوعية المياه الداخلة لمحطتي المعالجة والخارجة منها | The current study has been conducted on two types of waste water, industrial wastewater from Al - Dewaniyah textile factory station1, station 2 and municipal wastewater from Al - Dewaniyah wastewater treatment plant station 3, station 4 in the City of Al - Dewaniyah - Iraq., for the period from Spring2011 to Spring 2012 and targeted monitoring quarterly the quality of water entering and leaving the stations.The study also includes the evaluation of the efficiency of some algae and aquatic plants to improve water quality via bioremediation of some pollutants in the water. The evaluation of water quality includes18 parameters as confirmed on spatial and temporal changes of these parameters, as well as the algal contents of these four stations(S1, S2, S3and S4). The results of physical and chemical analysis show the following ranges of : temperature (12.5 - 41.5°C), total dissolved solids TDS(692 - 1384.6 mg/l), total suspended solids TSS(58 - 277.4 mg/l), electrical conductivity EC(1225 - 2045µS/cm2), pH(7.0 - 7.8), dissolved oxygen DO(0.7 - 8.51mg/l), biological oxygen demand BOD(48.82 - 205 mg/l), total alkalinity (170.7 - 428 mg/l), total hardness TH (412 - 1623.3 mg/l), calcium (89.7 - 204.14 mg/l), magnesium (37.7 - 332.5 mg/l), nitrite (0.43 - 12.25 mg/l), nitrate(6.18 - 63.75 mg/l), phosphate (0.813 - 20.24 mg/l), lead (0.0983 - 1.076 mg/l), chromium (0.007 - 0.045mg/l), cadmium (0.0374 - 0.326 mg/l) and the total algal number(17.00 - 14202 individual/l).The spatial and temporal variations show significant differences at(P?0.05) among studied factors in the different stations.The qualitative study of algae reports that the dominance is to the blue green algae(Cyanophyta)by 46%, then is followed by diatoms(Bacillariophyta) by 26% down with Dinophyta3%, according to Shannon - Wiener index of diversity (H), the fall and winter classified as severely polluted all around the stations 0.377 and 0.669, respectively, while summer and spring are moderate pollution of a category (II) and the values of (H) is1.374 and 1.395 respectively, in situ has been observed that the stations are classified as highly polluted and have been ranked within the category(I) and the stations arranged according to the pollution as S3 <S4< S2< S1.According to Species richness index the first station S1 is the best, follows by S4, S3 while S2 comes the last in order, but in the terms of total abundance has topped the third station S3 among the others, then followed by S4, S2 and finally S1. on the other hand the current study includs laboratory investigation to demonstrate the efficiency of algae and aquatic plants to improve water quality as it has been experimenting with immobilization of algae technique, a new method has been used for the first time at the local level in the current study. The results show that the immobilized algae Stigonema sp.and Cladophora glomerata have lowered the pH of the treated wastewater which began relatively high, as well as increasing dissolved oxygen concentrations at the end of the experiment all transactions free and immobilized treatments , on the other side TSS has been reduced about 61.8% when treated with immobilized algae C.glomerata, nitrite has also been reduced at about 96.7% and 85.5% by Stigonema sp.and C.glomerata respectively, nitrate also has been reduced by 99.9% and 95.2% when treated with Scendesmus dimorphus and C.glomerata respectively, while the removal of phosphate reaches its peak when it has been treated with free Stigonema sp. and immobilized S.dimorphus by 99.92% and 98.35% respectively.The tested algae shows a great ability to reduce heavy metals, when immobilized alga Oscillatoria sp. removes about 94.27% of Lead, while free Stigonema sp. and C.glomerata are the best in Chromium removal about 92.42% and 92.35% respectively, and the C.glomerata is the best in Cadmium processing, a reduced rate of 99.21% followed by immobilized S.dimorphus of 97.13%. The current study also involves a laboratory experiment to evaluate the potential of two aquatic plants duckweed (Lemna minor L.)and water hyacinth Eichhornia crassipes Mart.(Solms.) in improving the quality of wastewater the tested plants have been worked at increasing the aeration of treated water by raising dissolved oxygen concentration about 85.26% and 84.4% for duckweed and water hyacinth, on the other hand the relative efficiency to reduce the pollutants by aquatic plants as follows : EC, NO2, NO3(31.66% and 28.45%, 55.7% and 50.01%, 55.7% and 13.3%)for duckweed and water hyacinth respectively, from another side water hyacinth is the most efficient in phosphate reducing at rate of 90.5% comparing with duckweed 49.78%.On the of heavy metals treatment the tested plants show a remarkable removal of lead 88.23% and 80.4% , chromium 97.84% and 95.18% for duckweed and water hyacinth respectively, with respect to cadmium, water hyacinth shows high efficiency in the removal of this metal by 98.14%, while duckweed is the least in the treatment of Cd by 81.8%. From the above results, the current study demonstrates that there are a spatial and temporal variations regarding the factors examined in the stations of wastewater treatment units and confirms that the efficiency of studied wastewater treatment stations is below the required level, which contributes in increasing the pollution of Al - Dewaniyah River, which is the unique source to supply the city with freshwater for all purposes, that receives large loads of pollutants.The study also confirms that the evaluation of the presence of algae in the studied stations.Thus the knowledge of algal composition could give an early warning and accurate assessment about the quality of sewage before dumping into river. In addition to the forgoing, it is concluded that the biological treatment (bioremediation) of wastewater using free and immobilized algae and aquatic plants have improved dramatically and tangibly the benefits of the quality of that water being a promising approach of the wastewater treatment as well as being low cost economically.

حركية بعض العناصر النزرة في النظام البيئي لنهر الديوانية == Kinetic of Some Trace Elements In The Ecosystem of Al - Diwaniya River

اسم المؤلف: حيدر مشكور حسين
اسم المشرف: باسم يوسف الخفاجي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: The current study was conducted to understand the Relationship among concentration of selected of trace elements(Cadmium, Copper, Nickel, Lead and Zinc) in some trophic levels, water, and sediments, in Al - Diwaniya River. Samples were collected monthly for water, sediments, plant Phragmites australis (Cav.), phytoplankton, zooplankton, and two species of fish. from May (summer) 2013 up to April(spring) 2014 from three sites in Al - Diwaniya river. The current study included determine some physical and chemical parameters such as air and water temperature, pH, electrical conductivity, Total dissolved solids, Salinity, Light Penetration, Turbidity, Dissolved oxygen, the Biological Oxygen Demand, Total Organic Carbon of sediment, determined of the soil texture; and some trace elements in abiotic component such as water (dissolved and particulate), and in sediment, and so in biotic component included plant P. australis, phytoplankton, zooplankton, and two species of fish females (Cyprinus carpio) and (Tilapia zilli). The results showed that the air and water temperature ranged between (16.4039.55) ?C; (11.42 - 30.57) ?C respectively. The study also demonstrated that the pH values were neutral tend to light alkaline and ranged between (7.34 - 8.10), the electrical conductivity ranged between (7021 7021 - 7947 7947) µs/cm. while the TDS, ranged between (09.502 09.502 - 729950. 729950.) mg/l, according to the values of salinity ranged between (0.773 - 0.954)‰ the water of the river was classified as Oligohaline, while the values of Light Penetration ranged between (28.81 - 120.61)cm, and the turbidity ranged between (43.26 - 55.78) NTU, also showed dissolved oxygen as its value ranged between (45729 45729 - 7.979) mg/l, while the biological oxygen demand showed value ranged between (1.123 - 2.627) mg/l, The values of total organic carbon in sediments ranged between (0.440 - 1.045)%, While the sediment texture was sandy - silt in the first site, and sandy - clay in the both two and three sites of Al - Diwaniya river. Results of Concentration of trace elements (Cadmium, Copper, Nickel, Lead and Zinc) in dissolved part ranged between (252222. 252222. - 2522724 2522724), ( 252270 252270 - 252200 252200), ( 25227. 25227. - 252290 252290), (252224. 252224. - 2522022 2522022 ) , ( 25279. 25279. - 252010 252010 ) mg/l., whereas in particulate part ranged for between (0.29 - 1.49), (3.46 - 13.04), (12.51 - 20.99), (1.08 - 4.67 ((, (65.54 - 127.51 ) µg/g dry weight. and in sediments ranged between (0.53 - 1.96), (23.93 - 49.65), (136.85 - 166.09), (4.14 - 5.92), (126.69 - 176.64) µg/g dry weight respectively. In the Descending sequence in concentrations of all trace elements in abiotic components was as follow : sediments> particulate part> dissolved part. in producers of Al - Diwaniya river ranged in plant P. australis from (0.07 - 0.20), (5.93 - 16.21), (8.67 - 46.87 ) , (0.89 - 2.93), (26.60 - 103.78) µg/g dry weight respectively. in phytoplankton from (0.40 - 1.77), (8.89 - 25.2), (28.0666.49), (1.47 - 3.59 ) , (103.39 - 159.70) µg/g dry weight respectively. In the Descending sequence in concentrations of all trace elements in producers was as follow phytoplankton> plant P. australis. While the concentrations of trace elements in consumers in (zooplankton) of Al - Diwaniya river ranged from(0.56 - 4.41), (21.89 - 56.08), (51.64 - 152.79), (2.806.06), (137.14 - 183.61) µg/g dry weight respectively. While in two species of fish according to organs in gills of Cyprinus carpio ranged from(0.63 - 2.07) ) ) 3.19 3.90), ) ) 4.04 - 8.96) , (1.95 - 3.76), (182.14 253.89) µg/g dry weight respectively, in liver from(0.41 - 1.78), (6.20 - 32.47 ) , (5.97 - 10.39), (2.06 - 5.37), (136.15 184.75) µg/g dry weight respectively, and in muscles from (0.49 - 3.43و) , (و 2.82 10.40), (7.17 - 11.57 ) , (0.61 - 4.60 ) , (110.96 - 160.46) µg/g dry weight respectively. and ranged in gills of Tilapia zilli from(0.44 - 1.15), (1.06 - 6.83), (8.18 - 13.97 ) , (1.46 2.89) , (99.03 155.33) µg/g dry weight respectively, in liver from(0.571.64 ) , (13.08 - 28.06), ( 8.03 - 18.50), (1.32 - 3.02), (112.68 176.32) µg/g dry weight respectively, and in muscles(0.46 - 1.80), (2.42 2.93), (7.47 - 10.52), ( 0.20 - 3.16 ) , (79.74 - 153.58 ((µg/g dry weight respectively. concentrations of all trace elements in consumers always were higher than with producers and abiotic components In the Descending sequence in concentrations of all trace elements in consumers and producers was as follow : zooplankton> Cyprinus carpio> Tilapia zilli> phytoplankton> plant P. australis., And in the organs of fish was as follow in Cyprinus carpio : gills> liver>muscle, while in Tilapia zilli was follow liver > gills >muscle. While the values of the bioconcentration factor for (Cadmium, Copper, Nickel, Lead and Zinc) in producers of the study area ranged in plant P. australis. From 747542 747542)) - 7200500 7200500), ( 0.7150. 0.7150. - 729445.4 729445.4), (..1750...1750. - 047..59. 047..59.), ( 004540 004540 411507 411507), ( 44.592 44.592 - 4740509 4740509) respectively.In phytoplankton (7027512 7027512 - 000.2522 000.2522), (.704504.704504 - 79...549 79...549), ( 7111.547 7111.547 - 99420594 99420594), ( 7049542 7049542 - .20454..20454.), ( 0110527 0110527 77944502 77944502) respectively. the values of the bioconcentration factor for elements in consumers of the study area ranged in zooplankton from (712.59. 712.59. - .7444544.7444544), (0071504 0071504 - 42914504 42914504 ) , ( 0.004597 0.004597 - 07.700524 07.700524), ( 0..7527 0..7527 - 7709.544 7709.544), ( 4010577 4010577 70744574 70744574) respectively.in fish organs Cyprinus carpio ranged in gills from(197571 197571 - 97922522 97922522), (839.47 - 2808.33), (1704.16 - 4640.00), (1500.00 - 4076.92), (6876.10 - 13873.77) respectively. in liver ranged from (482.35 - 35600.00), (001.522 001.522 - 10075.00), (2473.81 - 6453.33), (1665.00 - 4646.15), (6373.16 - 12568.02) respectively. and in muscles (.14591.14591 - 40422522 40422522), ( 190572 190572 - 0444544 0444544), ( 7049500 7049500 9002500 9002500 - ) , ( 047520 047520 - 1214540 1214540), ( 4220501 4220501 - 9111502 9111502) respectively. while in Tilapia zilli organs ranged in gills (.71549.71549 - 00222522 00222522), ( 000500 000500 - 01015.2 01015.2) , ( 000254. 000254. 4070500 4070500), ( ((00475.0 00475.0 - 40.504 40.504 , (.40050..40050. - 0901541 0901541 ) respectively. in liver (4125.0 4125.000022522 00022522 ) , (.02.504.02.504 - 00000500 00000500), ( 0017590 0017590 - 77.405.2 77.405.2), ( 09457. 09457. - 9044500 9044500) , (499.5.0 499.5.0 - 0420592 0420592 ) respectively. and in muscles ranged from (.97571.97571 - 00022522 00022522), (771.05 - 0744544 0744544) , ( 7414574 7414574 - 401.522 401.522), ( ( ( 0.44500 0.44500 - 700502 700502, ( 5414.28 - 7354.09) respectively. results showed that the aquatic organisms (Consumers, Producers) have ability to bioconcentration of trace elements. and showed the descending sequence in concentrations of all trace elements in consumers and producers was as follow : zooplankton> Cyprinus carpio> Tilapia zilli> phytoplankton> plant P. australis. The result of this study confirms the biomagnification phenomenon (the increase of trace elements concentration with elevation in trophic levels of the food web) in study of Al - Diwaniya river ecosystem, as it's the case in other region of the world

دراسة تشريحية لسيقان وسويقات واوراق بعض اجناس العائلة الوردية rosaceae في الديوانية == Anatomical Study of The Stems, Leaves And Petioles For Some Species of Family Rosaceae

اسم المؤلف: حسنى مهدي رياح الفتلي
اسم المشرف: ازهار عبد الامير سوسة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: شمل البحث الحالي دراسة بعض الانواع العائدة للعائلة الوردية Rosaceae في محافظة الديوانية من الناحية التشريحية, اذ تم دراسة المقاطع المستعرضة للسيقان والسويقات والاوراق لثلاثــــــــــــة انــــواع وهـي المشمـــــش Prunus armenicana L. والكمثـــــــــري.Pyr | Current research involved the study of some species belonging to the family rosary rosaceae in Diwaniyah province of anatomical terms, as has been the study of cross sections of the stems, leaves and petioles for three species apricot Prunus. armenicana L. And pear Pyrus communis L. And apple Malus domestica Borkh. The study has been the separation of these species into groups depending on differences and similarities of the attributes of the obvious anatomical changes were discussed among these species, It turns out that many of these qualities, Especially the form of stem and cutworms as well as the presence or loss of tissue such as textiles Parencyma green Chlorenchyma in the stem as possible discrimination species P.armenicana for the other two species of loss of such a fabric, And also the possible discrimination of this species through the general shape of the clips transverse Soik as it's looks like a horse shoe, While the other two it's like a triangle. As for the indumentum and taxonomic diversity epithet to isolate and diagnose these species from each other, Such as Glandular hairs and the loss of these filaments in the other two species.The study concluded that the possibility of the use of the anatomical characteristics of transverse sections of the Stem, Petioles and Leaves in the diagnosis of the species under study

استجابة نبات السلق (Beta vulgaris var. cilca L.) للشد الملحي وحامضي البرولين والجبرلين == Response of Chard Plant (Beta Vulgaris Var. Cilca L.) To Salt Stress, Proline And Gibberellic Acids

اسم المؤلف: حيدر عبد الامير مظهور
اسم المشرف: انتصار حسين مهدي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: نفذت التجربة في الموسم الشتوي للعام (2012 - 2013) م في احد الحقول التابعة لناحية الحر في محافظة كربلاء, لمعرفة استجابة نبات السلق للشد الملحي والرش بحامضي البرولين والجبرلين والتداخل بينهما في بعض الصفات الكمية والنوعية لبعض الاجزاء الخضرية والتكاثرية (ا | An experiment was conducted in the winter season of the year (2012 - 2013) in a private Al - Hur district in Karbala governorate, to find out chard plant response to salt stress, proline and gibberellic acids and their interaction on some quantity and quality characteristics to some parts of vegetative and reproductive (leaves, flowers and seeds) to chard plant (Beta vulgaris var. cilca L.).The design of the experiment was randomized complete blocks (RCBD) in a factorial arrangement with three replicate. The first factor was four levels of saline water (0(River water), 2, 4 and 6) ds. m - 1 and the second factor was seven concentration of proline acid (PA) and gibberellic acid (GA3) (0(River water), 50PA, 100PA, 150PA, 50GA3, 100GA3 and 150GA3) mg. L - 1. Means were compared by using averages revised least significant difference (RLSD) at 0.05 probability level when the treatments referred to significant effect. The results showed : 1 - The negative effect of salinity levels on the qualities of the vegetative plant chard before flowering stage with the except of the dry weight of the root system, which had increased significantly with the level of salinity 2 ds. m - 1.2 - The negative impact of salinity levels in vegetative qualities of chard plant after flowering except root length, leaves chlorophyll content (a, b and total), nitrogen%, protein% and proline which increased with the level of salinity 2 ds. m - 1. as well as the potassium% in leaves with a salinity level 4 ds. m - 1 and dry weight of shoots with all levels of salinity and especially 6 ds. m - 1.3 - Gibberellic acid was dominant on proline acid in terms of chard plant by 150 mg. L - 1 concentration before flowering and 50 mg. L - 1. concentration after flowering while proline acid was dominant on gibberellic acid in dry weight before flowering and root dry weight of shoots after flowering, by 50 mg. L - 1 and potassium% in leaves and the protein% with 100 mg. L - 1 concentration.4 - The interaction between the salinity levels and acids concentrations add showed a significant difference in most of the traits of the plant, especially in combinations consisting of level 2 ds. m - 1 or comparison of saline with gibberellic acid by 150 mg. L - 1 before flowering and 50 or 100 mg. L - 1 after flowering while gave a combination of salinity level 4 ds. m - 1 with concentration of proline acid 100 mg. L - 1 higher of potassium% in leaves

انتشار انزيمات بيتالاكتاميز نوع OXA بين عزلات بكتيريا الزوائف الزنجارية في مدينة الديوانية == Prevalence of OXA Beta - Lactamaes of Pseudomonas Aeruginosa In Al - Diwaniya City

اسم المؤلف: رنا مشعل سالم
اسم المشرف: سيوف خومان علوان الرماحي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: The aim of This study is to determine the prevalence of OXA ? - lactamase in pseudomonas aeruginosa isolates from clinical and other environmental cases in Al - Diwaniya Teaching Hospital using phenotypic and molecular methods.The Samples of the study were collected from various sources and they were 390 samples for the period from November 2011 to March 2012, and they includ (292 clinical specimens and 98 environmental sample), the results of cultural and biochemical tests showed that 50 isolates (39 of them were from clinical cases and 11 were from environmental samples), belonged to ps. aeruginosa, and their diagnosis were confirmed by 16s - ribosomal RNA, and the study showed that all the isolates containing the 16s - ribosomal RNA gene, which represents the designed diagnostic gene in this study.The results of this study showed that the higher rate of P. aeruginosa isolates was due to burns infections (23.68 %) , followed by respiratory tract infections (sputum) that were (15.38 %) and then the case to otitis media infections (12.5 %), and lastly that due to urinary tract infections (10.49 %), while the rate isolation from the environmental samples was (5.11 %) from the floor of burns department, followed by (10.8 %) from the medical instruments of department workers.It was noted from this study that the age and gender of the patients have some effect on the infection by P. aeruginosa it was recorded that the higher rate of infection was in patients older then 61 year old, and regarding gender, it was found that females are more infected than male, especially in urinary tract infections. The study showed that there is high rate of infection in the admitted patients, in older people and in those who need urinary catheterization, in addition to those with severe burns and those in the intensive care units. Drug sensitivity test had been carried out for all the bacterial isolates to 22 types of antibiotics by disk diffusion method of Kirby - Bauer, and this study had indicated that there was relatively high resistance from P. aeruginosa to ? - lactam antibiotics, aminoglycoseides, and fluoroquinolone. The results also showed that the studied isolates has possibility of producing broad - spectrum ? - lactames enzymes, and this is supported by the resistance of this bacteria to the third generation cephalosporins and to Alaztronam, it was also shown that there was difference in the resistance to carbapenems antibiotics, the resistance rate to imipenem and meropenem were 16% and 64 %, respectively. Polymyxin B and Polymyxin E also called (Colistin) antibiotics were also used, and the resistance rate %0 for Colistin and %4 for Polymyxin B, that means there was two isolates which are resistant to Polymyxin B and this resistance was a challenge to the success of therapeutic efforts. this study noted that there was 22 (44%) isolates which were resistant to at least three types of antibiotics, and regarded as multi - drug resistant (MDR) and Extensive - drug resistance (XDR) isolates were26 (52%) which was the higher rate among the three types of resistance, The third type of resistance was 2 (4%) which represents the resistance to all types of studied antibiotics (PDR). The ability of these isolates to produce broad - spectrum OXA ? - lactames enzymes groups was investigated thrrough detection of presence of genes blaOXA - 10, blaLCR - 1, blaOXA - 18, blaOXA - 1, blaOXA - 2 in these isolates by using the polymerization chain reaction enzyme technology] (PCR) it showed 50/50 (100%) isolates contain blaOXA - 10 gene, which belongs to the OXA group I, and the results of this study showed no amplification results for blaOXA - 18, blaLCR - 1, blaOXA - 1, blaOXA - 2 genes, which belong to the main groups of broadspectrum OXA ? - lactames enzymes which are OXAgroup II, OXAgroup III and OXA groupV respectively except OXA18 enzyme

تاثير المعاملة ببعض مبيدات الافات والمواد الكيمياوية في نمو الفطرين Alternaria tenuissima وFusarium oxysporum المرافقة لبذور وجذور الحنطة Triticum aestiumn == Effect of Some Pesticides And Chemicals In The Growth of Fungi Alternaria Tenuissima And Fusarium Oxysporum Associated With The Seeds And Roots of Wheat (Triticum Aestiumn)

اسم المؤلف: مهند جاسم حمود
اسم المشرف: عبد الامير سمير سعدون
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اذ تم في هذه الدراسة عزل عدة انواع من الفطريات المرافقة لجذور وبذور الحنطة تموز 2 مصدق 1وتم تشخيص تسعة انواع من الفطريات المرافقة للبذور وبنسب تردد مختلفة وهذه الانواع هي Aspergillus niger, Penicillium notatum , Alternaria tenuissima, Rhizopus stotanifer | As it was in this study to isolate several types of fungi associated with the roots and seeds of wheat July 2 certified 1 was diagnosed nine types of fungi associated with seeds and different frequency rates and these types areAspergillus niger, Penicillium notatum, Alternaria tenuissima, Rhizopus stotanifer P. digitatum, Fusarium oxysporum, A.flavus, Fusarium solani, A. alternata. Was diagnosed nine types of fungi isolated from the roots of wheat and the surrounding soil and different frequency rates and these types. A. alternata, A. niger, R. stolanifer, Mucor sp., Trichoderma hazianum, P. notatum, F. oxysporum, F. solani, Alternaria tenuissima. Testing was diagnosed two fungal species tested using a technique monomer chain reaction PCR. These species are fungal Fusarium oxysporum, Alternaria tenuissima. This study tested the effect of pesticides is a fungal pesticide jungles Granstar and insecticide Wide. The transactions chemical which urea and NaCl in the growth of some fungi isolated from the seeds and roots of wheat as it has been test the effect of different treatments and their interaction in the germination of seeds of wheat on the filter paper and compare nets comparison innate Elsa (Carbendazim) and underwent tests the effects of different treatments and their interaction in radial growth and dry weight of the two types of fungi isolated in the germination of their spores and bacterial length of the tube, as well as test the effect of transactions and their interaction in the germination of seeds in the soil sterile and non - sterile. The results showed that the use of urea and sodium chloride concentration of 15 mg / mL in the treatment of seeds of wheat led to increased germination of these seeds and the impact of a negative in some types of pathogenic fungi associated with them as well as the use of pesticides other than fungal pesticide jungles Granstar and insecticide Wide negative effect also in the vitality Activity and fungi.germination The results showed the existence of significant effects on rates of seeds treated with pesticides and salts on the filter paper analogy transactions comparison since given concentration 15 mg / 10 mL of pesticides and 15 mg / ml of salt are high in the germination of the seed treatment, which exceeded ratios germination of seeds of wheat treatment urea fertilizer treatment fungicide Elsa. As rate ratios germination of wheat seed treatment with urea 96.66%, while the rate ratios germination of seeds of wheat pesticide Elsa 93.33%, followed by treatment of pesticide Wide as rate ratios germination 83.33% and 80.00% for wheat seed when the focus 15 mg / ml of sodium chloride salt treatment, and after treatment Karan Star as the rate of germination rates of seeds of wheat 73.33%. The results showed the existence of significant effects in increasing the proportion of seed germination treatment with pesticides and salts in the soil sterile and non - sterile as it gave the Focus 15 mg / 10 mL of pesticides and 15 mg / ml of salts highest rates of germination of these seeds came these results are compatible with the results of seed germination in the filter paper. The results show that as well as the individual transactions were more influential than the transactions overlap in radial growth and dry weight of fungi testers and their spores germination rates and lengths of germ tubes, as growth rates of radiographic Fungi F. oxysporum and A. tenuissima in the treatment of a mixture Wide and Granstar 11.44, 8.55 mm, respectively, while the rates of diameters of the colonies for the treatment of urea pesticide Wide 33.66 and 26.66 mm, while the pesticide mixture Wide treatment with sodium chloride amounted to 37.00 and 15.00 mm, in while rates were diameters of colonies for the treatment of a mixture Granstar with sodium chloride, 52.66 and 25.00 mm, and the growth rates were radiography for the treatment of pesticide Granstar with urea 25.00 and 11.66 mm, and the growth rates for the treatment of sodium chloride with urea 31.24 and 37.33 mm, compared with the fungicide Elsa, which amounted to the radial growth rates of 8.55 - 11.44 mm Fungi testers respectively.

تاثير الاجهاد المائي والسماد الورقي والمخصب العضوي في نمو نبات حلق السبع الشجيري Adhatoda vasica (L.) Nees ومحتواه من بعض المواد الفعالة وفعاليتها المضادة في بعض بكتريا تسوس الاسنان == Effect of Water Stress, Foliar And Organic Fertilizers On Growth of Adhatoda Vasica) L.(Nees. And Its Content of Some Active Ingredients And Inhibition Activity of Some Dental Caries Bacteria

اسم المؤلف: ليث سريع الركابي
اسم المشرف: ثامر خضير مرزة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: This study was conducted in Biology Department - College of Science - University of Al - Qadisiya that included pot experiment from the period of 1/4 to 1/8/2013, to assess the effect of water stress, foliar fertilizer (Foltron) and organic fertilizer (Azomin) on growth of Adhatoda vasica (L.) Nees and Its Content of Some Active Ingredients. Laboratory experiments were also done from the period of 1/8 to 1/3/2014, to determine the activity of leaves and roots extracts that resulted from plants of pots treatments to inhibit growth of some bacteria genera causing Dental Caries i.e. Staphylococcus aureus, Streptococcus mutans and Streptococcus pneumonia.A pot experiment included seventy two transplants (age of one month and 8 cm height) that were transplanted in plastic pots (one transplant per pot) in 1/4/2013. Pots were filled with sandy soil and peatmose 2 : 1 v/v. Plants Irrigated with two levels 50 and 25% of field capacity. Foliar fertilizer was used at three concentrations of 0, 3 and 6 ml/L that sprayed twice on plant shoot till complete wetness during 15/4 and 1/6/2014. Organic fertilizer was also used with irrigation water at three concentrations of 0, 4 and 8 ml/L twice on 1/5 and 15/6/2014. The design of the experiment was Randomized Complete Blocks Design (RCBD) in a factorial arrangement (2×3×3) with four replications per each treatment. Revised Least Significant Difference (RLSD) was used to compare treatment means at probability 0.05 level when treatment effect was significant.Results of pots experiment were as follows : 1 - Increasing water stress level caused a significant decrease in the most characteristics of vegetative and root growth except for stem internode and root lengths, while increasing in concentration of foliar and organic fertilizer gave the opposite effect of water stress.2 - Water stress caused a significant decrease in leaves content of total chlorophyll and nutrient elements N, P, K, Mg and protein percentage, while foliar and organic fertilizer caused a significant increase on the above characteristics.3 - Water stress caused a significant increase in proline percentage and Vit.C. and Vasicine and Vasicinone alkaloids, while foliar and organic fertilizer caused a significant decrease in proline percentage and Vit.C. and a significant increase in Vasicine and Vasicinone alkaloids.4 - The treatment of plants that were subjected to the water stress via foliar or root fertilizing or both of them caused a significant increase for most of vegetative and root growth characteristics, total chlorophyll, nutrient elements, protein percentage and Vasicine and Vasicinone alkaloids.5 - The treatment of plants that were underwent to the water stress via foliar and root fertilizing caused a significant decrease in proline percentage, Vit.C. and stem internode and root lengths.6 - The interaction of the three studied factors gave a significant positive effect to rise alkaloids of plant.Laboratory experiments included picking fresh leaves and root of plants that dried aerial in a shade, and then extracted it via methanol alcoholic of 70%, it was detected for some chemical components of extract by using reagents. Bacterial genera were isolated from the surfaces of the teeth and cultured on the growth media, and it was diagnosed by the Vitek Compact 2 System Diagnosis. The activity test involved the impact of the alcohol extract for leaves and roots to the inhibition of bacterial growth by the agar well diffusion method on Muller - Hinton.The design of the experiment was Complete Randomized Design (CRD) in a factorial arrangement (18×3) with three replications per each treatment. Revised Least Significant Difference (RLSD) was used to compare treatment means at probability 0.01 level when treatment effect was significant.Results of laboratory experiment were as follow : 1 - Increasing extracts concentration caused a significant increase in growth inhibition of the studied bacterial genera.2 - plants extraction that of the combination of A2B3C3 included (25% water stress, 6 ml/L foliar fertilizer and 8 ml/L organic fertilizer) gave the highest activated inhibition for bacterial genera compared with plant other extraction for all tested bacterial genera.3 - The plants extracted that treated by combination A2B3C3 in concentration 150 mg/ml gave higher activated inhibition for Streptococcus pneumonia reached 25.307 ml compared with Amoxicillin that reached 36.167 ml, while the inhibited extracted root reached for the same treatment 16.060 ml (Table 37 and 38).4 - Plants leaf extract was the highest effective inhibition of bacterial compared to the roots extract.

تاثير بعض عوامل المكافحة الحيوية في بعض الجوانب الحياتية للذبابة المنزلية Musca domestica L. (Diptera : Muscidae) == Effect of Some Biological Control Agents On Some The Biological Aspects of Musca Domestica (Diptera : Muscidae)

اسم المؤلف: علي مرتضى كاظم الياسري
اسم المشرف: محمد رضا عنون الحسناوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية تقويم كفاءة بعض طرائق السيطرة الحيوية باستعمال الفطر Sorokin Metschnikoff)) Metarhizium anisopliae والبكترياBacillus thuringiensis var.israelensis ومنظم النمو Neporex Cyromazine) ) في المكافحة الحيوية للذبابة المنزلية Musca domestica | The present study aimed to evaluation of efficacy of some methods Metarhizium anisopliae Sorokin) Metschnikoff) Bacillus thuringiensis var.israelensis and Neporex) Cyromazine) from be used as biocontrol agents against (Diptera : Muscidae)Musca domestica L. The results showed the following : 1. Different concentration of Fungal suspension have been affected the life stages of Musca domestica. The mortality rate of eggs were (18.66%) at the concentration of 2 × 103 spore /ml, and increased to (36.66%) at the concentration of 2 × 106 spore /ml. The first larvae instars have showed highest mortality rate reached (83.33%) when treated with 2 × 106 spore/ml, while (56.66%) of them were dead at concentration of 2×103 spore/ml. (36.66%) of pupae of Musca domestica were dead when exposed to 2 × 106 spore /ml. The treatment with highst concentration caused high adult mortality, i.e. (96.66%) and (93.33%) for adult males and females of the Musca domestica respectively after 168 hours, while (70%, 73.33%) when treated with 2×103 spore /ml. 2. The secondary metabolities of fungi when applied, all first larvae instars of Musca domestica were dead and (96.66%) of the when tretead with concentration 100%, while (66.66%) and (63.33%) for the same larval instars at concentration 25%. 3. The result showed the different concentration of bacterial suspension affected the mortality rate of larvae of Musca domestica.The first larva instars have showed highst mortality rate which reached (76.66%) when treated with 2 × 105 spore /ml while (50%) of them where dead at concentration 2 × 102 spore /ml after 72 hours from treatment. 4. The effect of secondary metabolites of bacteria when applied, All first larva instar of Musca domestica were dead and (90.00%) when treated with concentration 100% and decreased to (73.33%) after 72 hours from treatment , the mortality rate of adult was (100%) at concentration 100% and decreased to (63.33%) after 72 hours from treatment. 5. The concentration of insect growth regulator neporex have effected , The highst mortality rate eggs was (37.00%), at the concentration 100 part per million, while (19.33%) at the concentration 25 part per million, , the first larval instars mortality at the concentration 100 part per million, was (96.66%) at the concentration 100 part per million while (66.66%) at the concentration 25 part per million.the mortality rate of pupa was (73.33%) while (30%) at the concentration 25 part per million after 72 hours from treatment

دراسة بيئية للطحالب الملتصقة على بعض النباتات المائية في مياه مبزل الفرات الشرقي (الحفار) / الديوانية - العراق == An Ecological Study of Epiphytic Algae On Some Aquatic Plants In East Euphrates Drainage Water /(Al - Haffar) Al - Diwaniya/Iraq

اسم المؤلف: زهرة كليب مهدي الخزعلي
اسم المشرف: فؤاد منحر علكم
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات - الاحياء المائية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تناولت الدراسة الحالية الخصائص البيئية المؤثرة على الطحالب الملتصقة على النباتات المائية في مبزل الفرات الشرقي (الحفار) في الديوانية - العراق, اذ شملت الدراسة قياس بعض الخصائص الفيزيائية والكيميائية لمياه المبزل والخصائص الاحيائية التي تضمنت دراسة كمية ون | The present study tackles ecological characteristics which is effected of Epiphytic algae in east Euphrates drainage (AL - Haffar) in AL - Diwaniya, This study included the measurement some of physical and chemical characteristics of drainage water, and biological characteristics which includes studying quality and quantity of Epiphytic, Three aquatic plants Ceratophyllum demersum, Phragmits australis, and Imperata cylindrical had been selected, Samples collected monthly during The period from November 2012 to October 2013.Three location have been chosen, the first is near AL - Abtan village, Second is after the first location five kilometers, while the third location is after the Second about seven kilometers. The study shows that the temperature of the air is about (7 - 39c) and water temperature is approximately (10 - 33c), while light penetration is about (10.5 - 63cm) the flow speed is (0.25 - 0.68 M/Sec), Water turbidity is (40.02 - 63.03 NTU), while Electrical conductivity is approximately about (3014 - 3964 Sc/cm). The results of the study shows that the water drainage Oligohaline, the salinity values ranged from (1.92 - 2.53‰), the total (T.D.S) is about (1741 - 2226 mg/l), while the T.S.S. is about (41 - 67 mg/l). The result of this study shows that the pH is limited Through all the period of the study (6.98 - 8.18), The drainage water get good airing dissolved oxygen values are(6.71 - 8.14 mg/l), biological oxygen demand are (2.05 - 5.23mg/l) and carbon dioxide values are about (81.50 - 119.57mg/l).The results of this study also show about that drain water is alkaline and belonged to bicarbonate ions, total alkaline is (140.28 - (189.27 mg/l) It is discovered that the drain water is too hard, hardness values are (512.15 - 612.45mg/l), while Calcium concentrations are (128.36 - 173.40mg/l) and Magnesium concentrations are (30.31 - 50.09 mg/l). Plant nutrients, N concentrations are about (0. 98 - 3.99 µg/l) of Nitrit, Nitrate concentrations are (216 - 415 µg/l) and Phosphate concentrations are about (0.9 - 2.92 µg/l), and The percentage of total organic carbon in the residues is(0.59 - 0. 17) %. The total number of epiphytic algae types which are diagnosed during study period reaches to 320 species belongs to 86 genera within 7 classes They are Bacillariophyceae, Chlorophyceae, Cyanophyceae, Euglenophyceae Xanthophyceae, Dinophycea eand Rhodophyceae, Bacillariophyceae obtaints the dominion which reach to (183) species of Bacillariophyceae. It makes rate (57.19%).Than Chlorophyceae came concerning types number 68 species in rate (21.25%) , Following by Cyanophyceae 53 species in rate 16.56%. Euglenophyceae are 10 species in rate (3.13%).Xanthophyceae is represented in two species belong to2 genera.It makes proportion (0.63%) , Dinophyceae which represented in 3 species belonged to 3 genera in rate (0.94 %) and Rhodophyceae represented in one species and one genera in rate (0. 3%) of total number of algae.Class of the Bacillariophyceae, during study period were Navicula وNitzschia وCymbella وSynedra وAchnanthes, genera of Oscillatoria and genera of, Scenedesmus manifest that highest number of available types during the period of study.It has note that there are numbers of the monthly and located changes in total number of epiphytic algae on host plants.was varied in months and locations due to the host plants. Ceratophyllum demersum marks the Highest total number of epiphytic algae 172.89× 104 individual/ gm of wet weight in third location during April. Imperata cylindrica marks the lowest total number of epiphytic algae 3.51×104 individual/ gm of wet weight in scecond location during January, The total number of epiphytic on host plants ranges between (175.39 - 668.6) ×104 individual/ gm of wet weigh.The total number of main epiphytic algae classes is varied in total number of epiphytic algae.Bacillariophyceae diatoms composed 81.02%, in all locations during the study period, followed by Cyanophyceae composed 9.7%, while Chlorophyceae composed 8.6%.Euglenophyceae, Chrysophyceae, Dinophyceae and Rhodophyceae, were not important concerning their number. Statistical analysis results show there are significant differences between locations and months of study in relation to physical, chemical and biological features. Also there are connected correlations positive and negative between epiphytic algae and physical, chemical and biological features that measured during the study period of probability level of P<0.05.

دراسة نسجية مقارنة بين عضلات الصدر والارجل في نوعين من الطيور الطائرة وغير الطائرة في عمرين مختلفين == Comparative Histological Study Between Pectoralis And Leg Muscles In Flying And Running/Walking Birds In Two Different Ages

اسم المؤلف: تغريد حامد عبد الامير
اسم المشرف: هاشم محمد عبد الكريم العلاق
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تناولت هذه الدراسة عددا من العضلات الهيكلية في نوعين من الطيور الطائرة (الحمام) وغير الطائرة (الدجاج). العدد الكلي للطيور المستعملة 30 طائرا, 15 من الدجاج و15 من الحمام تمت دراسة بعض الخصائص الفسلجية والنسيجية للالياف العضلية الحمر والبيض والوسطية وحساب | This study deals with a number of skeletal muscles in two types of birds ; flying (pigeon) and running (chicken) types. The total number of the studied birds was 30; 15 were chicken and 15 were pigeons. Some of the physiological and histological characteristics of the muscle fibers (red, white, and intermediate) , the percentage of fibers diameter in the studied muscles, and the change noted in the muscle weight with advancing age had been calculated. The species of the chicken in this study was Gallus domesticus , while the species of the pigeons was Columba livia domestica. The muscles that is used in the study were : pectoralis muscle, femortibialis, triceps, biceps brachii, extensor carpi radialis muscle, and gastrocnemius muscles in two age groups of birds; (3 - 6) months and (6 - 12) months in order to know the changes in the weights, numbers, diameters, and sizes of the muscle fibers that occur with advancing age. The aim of this study is to identify the three types of the muscle fibers their role in the muscle movement and the regions in which there is specific type rather than others. The results had shown that the skeletal muscles in birds consist of heterogenous mixture of the three types of muscle fibers (red, white, and intermediate) , and the white muscle fibers are the predominant type in the pectoralis muscle of both types of birds, and the size of white muscle fibers in the pectoralis muscle in the chickens in the first age group was 73.8%, and in the second age group was 82.9%. While in pigeons, the size of white muscle fibers was 75.5% in the first age group, and 75.4% in the second age group. In addition, the present study deals with the relationship between the muscles weights and the age of the bird in that the muscles show increased weight with advancing age in both types of birds and there were significant differences in muscles weight in both age groups (p?0.05), while the triceps muscle did not show significant difference regarding weight between the two age groups in the same type of birds. The results of the study also show the differences in the diameter of the muscle fibers, that is there was continuous increase in the diameters with advancing age and show significant differences (p?0.05) , but regarding pectoralis muscle there was no significant differences in diameters with advancing age in both types of birds. The results of the study also show that the pectoralis muscle was the largest muscle in the body of the bird, and the ratio of its weight to the total body weight was relatively large.

دراسة تشريحية لبشرة اوراق بعض اجناس العائلة المركبة Compositae (Asteraceae) في جامعة القادسية == Anatomical Study of The Leaves Some Genus Compositae (Asteraceae) In Al - Qadisiyah University

اسم المؤلف: فرقد حيدر عبد النبي المحنة
اسم المشرف: ازهار عبد الامير سوسة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تناول البحث الحالي دراسة تشريحية مقارنة لصفات البشرة للاوراق بعض الاجناس التابعة للعائلة المركبة (Compositae) وهذه الاجناس هي Aster L. و.Calendula L وDendranthema L. و.Helianthus L و.Lactuca L و.Launaea L و.Tagetes L, وتبين ان لبعض الصفات التشريحية اهمية | The present research includes comparative anatomical study epidermis of some genera which belong to sun flower family (Compositae) These genera are Aster L., Calendula L., Dendranthema L., Helianthus L., Lactuca L., Launaea L. and Tagetes L. , and show that details anatomical the importance of taxonomic possible from isolation genuses more each adjective undulate the walls vertical epidermis the leaves, and we show some importand for indumentum suchas T - shape eglandular haire in Dendranthema L. species

التحري عن جينات المقاومة لبعض المضادات في Candida spp. المسببة لداء المبيضات == Detection On Resistant Genes To Some Antifungal In Candida Spp. That Causing Candidiasis

اسم المؤلف: رجاء علي حبيب ظاهر الخزاعي
اسم المشرف: علي حمود السعدي | نيران عبيد جاسم
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تم جمع 107 عينة من حالات سريرية مختلفة شملت 36 مسحة فموية من اطفال مصابين بالسلاق الفموي, 32 مسحة مهبلية من النساء المصابات بداء المبيضات المهبلي و39 عينة بولية من اشخاص مصابين بالتهاب المجاري البولية (راجعوا مستشفى الديوانية التعليمي العام ومستشفى النسائ | The present study was aimed to determine ERG11 - 2 that responsible for resistance to some antifungal agents in candida spp. Atotal of 107 samples were obtained fromdifferent clinical cases included(36 oral swabs from children suffering from oral thrushwho attending Al - Dewaniya Maternity and Pediatrics Teaching Hospital in AL - Diwaniya, 32 vaginal swabs from women suffering from vaginal candidiasis who attending clinics in AL - Diwaniya, 39 urine samples from individuals suffering from urinary tract infection who attending Al - Dewaniya Maternity and Pediatrics Teaching Hospital in AL - Diwaniya) and 25 samples from sewage water as a control samples in the molecular study.The result of morphological and biochemical tests showed For pathogenic samples that 83(77.5%) isolate of candida spp. Included 36/31(86.1%) from oral samples, 32/28 (75%) from vaginal swabs, 28/39 isolates (71.7%) from urine samples while the result of non - pathogenic samples revealed that 20 (80%) isolate of candida spp. Detecting of, ERG11 - 2 resistant gene was done by RealTime - PCR technique. Results showed that all isolates 31 (100%) were carrying ERG11 - 2 that responsibe of resistance in candida to some antifungal, Did not record any isolated from control samples from sewage water which amounted to (9) the ERG11 - 2 resistant

دراسة جرثومتي السالمونيلا والشيكلا المعزولة من حالات سريرية في مدينة الديوانية وتاثيرهما على معايير دم المرضى

اسم المؤلف: هبة اركان فنطيل الحسناوي
اسم المشرف: علي عبد رحيم الناشي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: Salmonella is a bacteria and Shigella types of intestinal bacteria, and because of the seriousness of the pathogenicity and the spread of infection in their environment has been conducting this study.Collected during the current study, 462 different clinical sample (feces and urine) of adults aged 20 years and older and those with symptoms of the bacteria Salmonella and Shigella and both sexes reviewers for laboratories and hospitals in the city of Diwaniya, for the period from 2012 \ 11 \ 6 to 2013 \ 5 \ 26. Diagnosed isolates based on phenotypic traits and AGRO tests biochemical in addition to diagnosis by Api20E system as isolates Salmonella reached 17 isolation and by 3.6% and were all belonging to the type S.typhi, either isolates bacteria Shigella was three isolates and 0.64%, and all of them belong to the type of Sh. Flexneri. The incidence of bacteria S.typhi for the male sex was higher than in females, Ozbulg number of infected males and 10 by 58.8%, while the number Fayalnads was infected 7 and by 41.1%, but for bacteria to Sh. flexneri, as was the incidence in males is also higher than in females as in males reached 2 and by 66.6%, while the number of infected females in bringing only one injury and 33.3%. Were more age groups injury is a class 20 - 29 where recorded 9 injuries and by 52.9% and the number of infected males 6 The number of cases in females 3 injuries, age group 30 - 39 recorded 5 injuries and by 29.4 and were two goals in the male and three injuries in females, the age category 40 - 49sgelt two goals by 11.9% and the injury was confined to males either category above 50 years and one injury recorded only in females and 5.8%, either in bacteria Sh. flexneri was the only injury in the 20 - 29 age group and by three injuries. The more isolated the proportion of bacteria S.typhi in the month of May and April as recorded 4 isolates each, and by 23.5%, while giving the lowest ratio for the presence of bacterial during the months of December and January, as recorded isolation and one each of these two months and 5.8% either November remaining months February, March was the number of isolates in 3, 2, 2, respectively, and 15%, 17.6%, 11.7, 11.7%, respectively, as well, either for the bacteria Sh. flexneri as Bulgtaaly proportion of injury in the month of May was the number of isolates 2 and by 66.6% during the month of April either recorded a hit single Vqtobnsph 33.3% did not notice any injury to the other months of the study. Tested the sensitivity of the isolates S.typhi and Sh.flexneri direction antibiotics used as 8 used antibiotics in common use in this study show that the bacteria were sensitive S.typhi 100% Anti Ciprofloxacin, but resisted antibiotics Ampicillin, Amoxicillin, Chloramphenicol, Tetracycline, Nalidixicacid, Gentamicin, Ceftriaxone at a rate of 82.3%, 82.3%, 76.4%, 70.5%, 64.7%, 23.5%, 23.5% respectively. The bacteria Sh.flexneri was 100% sensitive to antibiotics Ciprofloxacin, Gentamicin, Nalidixic acid, Amoxicillin, Ceftriaxone while it was resistant to antibiotics Tetracycline, Chloramphenicol, Ampicillin by 66.6% each. Been investigating some of the virulence of the bacteria S.typhi factors, including the presence of the portfolio Capsule as were all Salmonella isolates characterized by possessing the portfolio, while the production of the enzyme protease Protase was all isolates S.typhi producing this enzyme also showed 12 isolation of the ability to produce the enzyme Lipase and increased by 70.5%, but all isolates were S.typhi unproductive enzyme protease. The bacteria Sh.flexneri marked by Azltan of three Azladtha and by (% 66.6) viability link red dye Congo also showed 2 of these isolates and by (66.6) ability to form thin membrane (Pellicle) and two Aezzltan which have demonstrated susceptibility to adhesion was isolated one of them only possess cilia first pattern type 1. The research covered also study the changes that occur in some of the blood parameters in people infected with the bacterium Salmonella Heclauzar that there is a decrease in the total number of red blood cells Red blood cells in people infected with Salmonella, where the total number of blood cells Reds reached 4.91 x 106 c \ ml total number of cells rate 3.7 white blood x 103c \ ml the proportion of types of white blood cells of the total number of cells, the white was lymphocyte ratio lymphocytes% 52.55 which is low compared with the normal number, while there was an increase in both acidophilus cells Eosinophils and only Monocyte as she was represented 1.2%, 2.9% in November while it was% 0.03 Basophils cells, the highest percentage of increase in healthy people, and the amount of total hemoglobin rate. Hemoglobin9.07 g \ dl which is low compared with healthy peopleBut in people infected with bacteria Sh. Flexner, bringing the average number of red blood cells 106 c \ ml3.7 x, the lowest rate with the number of healthy people, as the amount of hemoglobin g \ dl13.5 rate, either the total number of white blood cells, the rate was slightly higher than what is found in healthy people Azbulg (11.32 x 103c \ ml), it was noted that most of the increase was high in the white blood cells of the type Neutrophils the increase amounted to 62.34%

دراسة التاثير الاليلوباثي لنبات اليوكالبتوس Eucalyptus sargentii في انبات ونمو بعض نباتات الزينة == The Allelopathic Effect of Eucalyptus Sargentii On Germination And Growth of Some Ornamental Plants

اسم المؤلف: كوثر هاشم عبار الجاسمي
اسم المشرف: مجيد كاظم عباس الحمزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت تجربتين, حقلية ومختبرية, لدراسة التاثير الاليلوباثي لمسحوق الاوراق الجافة او الطرية لنبات اليوكالبتوس ومستخلصاتها (المستخلص الايثانولي, الزيت, التربينات, القلويدات والفينولات) في الانبات وصفات النمو الخضري والزهري ومحتوى بعض العناصر المعدنية, السكري | Two experiments were carried out, field and laboratory experiment, to study the allelopathic effect of dry or fresh leaves and their extracts of Eucalyptus sp. on four ornamental plants; Calendula officinalis, Tagetes patula, Mathiola incana and Asteriscus graveolens Less. The two experiments were conducted at the field of College of Agriculture/Al - Qadisiya University during the period from 31/10/2012 to 14/3/2013. For the field experiment, dry and fresh leaves of Eucalyptus were used at the rate of 0, 2, 3, or 6 g dry leaves/Kg soil or at the rate of 0, 4, 8, or 12g fresh leaves/kg soil. Plant height, changes in height during plant life time - course, number of branches, number of flowers, and flower diameter were measured. In addition, chlorophyll content in leaves, anthocyanin in petals, dry weight, nitrogen percent, potassium and phosphorous content in leaves were also determined. For the laboratory experiment, ethanol extract, oil, terpenes, alkaloid, and phenols extracted from Eucalyptus leaves at different concentrations were used. Seed germination, coefficient velocity of germination, plumule and radical length, sugar, protein, melanodialdehyde, glutathione and proline content were evaluated. The results were as follow : 1. Ethanolic extract was negatively affect the percent and coefficient velocity of germination in addition to plumule and radicle length. The reduction in these parameters was greater at the highest concentration of the extract, especially in Calendula officinalis. 2. Treatment with oil extracted from Eucalyptus leaves caused a significant reduction in percent and coefficient velocity of germination, plumule and radical length. 3. Terpenes, alkaloids or phenols treatments at different concentrations resulted in significant reduction in percent and coefficient velocity of germination, plumule and radical length. The reduction was increased as the concentration of the treatments increased. 4. Plant height decreased significantly due to the treatment with dry or fresh Eucalyptus leaves, the reduction in plant height increased as the concentration of the treatment increases in all plants except Asteriscus graveolens plant which showed that the small concentrations of both treatments. It was close to ward in an increase in plant height. 5. Number of branches also significantly decreased when ornamental plants treated with dry or fresh Eucalyptus leaves; the Calendula officinalis plant was the most affected. 6. For the changes in ornamental plants height, as a result of treatment with dry or fresh leaves of Eucalyptus, during life time - course, it was clear the growth curve was close to the S - shape growth curve with obvious significant differences for most treatments compare to control. 7. Dry weight percent was significantly affected in all ornamental plants when they treated with dry or fresh Eucalyptus leaves, If decreased with the concentration of the treatments increased. 8. It was obvious that treatment with dry or fresh Eucalyptus leaves resulted in significant reduction in flower number and flower diameter. Number of flowers decreased as the concentration of the treatments increased. on the other hand, flower was diameter increased as the concentration of the treatments increased, but in both cases it was less than the control. All one obvious that reducing significant in flower diameter, It was reducing great in low levels. 9. Chlorophyll content was also decreased in leaves, while anthocyanin content in petals was increased when the ornamental plants treated with dry or fresh Eucalyptus leaves. 10. Nirogen percent, phosphorus and potassium content in leaves of the four ornamentals decreased when they treated with dry or fresh Eucalyptus leaves. 11. Using terpenes, alkaloids and phenols caused significant increase in sugar content in leaves of the ornamentals, reaching its highest values at the highest concentration of the terpenes. For protein content, it was gradually decreased as the terpens, alkaloids and phenols concentrations increased in Calendula officinalis. In Tagetes patula, protein content was noticeably increased with the increasing in concentrations of the treatments. 12. Malondialdehyde, glutathione and proline content increased significantly in Calendula officinalis and Tagetes patula plants when they treated with terpenes, alkaloids or phenols. 13. Interaction between treatments concentrations and kind of plants had significant effect on all parameters measured.

دراسة تركيبة الهائمات النباتية وعلاقتها بالظروف البيئية وبعض العناصر الثقيلة في مياه نهر الديوانية - العراق == A Study of Structural Phytoplankton And Their Relationship With The Environmental Conditions And Some Heavy Metals In Al - Diwaniya River /Iraq

اسم المؤلف: نور ناظم جابر الميالي
اسم المشرف: فؤاد منحر علكم
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تناولت الدراسة تركيبة الهائمات النباتية وعلاقتها بالظروف البيئية وبعض العناصر النزرة في مياه نهر الديوانية, اذ جمعت عينات الدراسة (المياه والرواسب والهائمات النباتية) شهريا من ثلاث محطات على نهر الديوانية ابتداء من تشرين الاول 2012 ولغاية ايلول 2013، وشمل | This investigation was designated to study of structural of phytoplankton and its relationship with environmental conditions and some of heavy metal in AL - Diwaniya river, the samples were collected monthly for water, sediments and phytoplankton from October 2012 till September 2013, the study included the measurement of the temperature of air and water; Light penetration; salinity ;electrical conductivity ; T.S.S;T.D.S ;Turbidity; pH ;Oxygen Dissolved; Biological Oxygen Demand ;Chemical Oxygen demand ;Dioxide carbon ;AL - alkalinity; Hardness; Ca; Mg; and nutrients(nitrate; nitrite; phosphate) ; total organic carbon) and the study also included the measurement of some heavy metals (Cadmium, lead, copper, Zinc) in dissolved and particular parts of water and in sediment both exchangeable and residual parts and also studied the structure of phytoplankton quantity and quality.Results of the study showed that the temperature of air and water ranged between (15 - 43) ?C (13 - 33)?C respectively. It was found the light penetration ranged from (27.2 - 100), conductivity was (1264 - 1555)?S/cm, while the salinity was (0.80 - 0.99)%, pH was ranged between (7.37 - 7.82), while Turbidity was ranged between(43.6 - 59.02) NTU, total dissolve substances was (985 - 1411) mg/L while total suspend solid substances was (40 - 66) mg/l. The dissolved Oxygen was within the range of (6.3 - 8.26) mg/l, the biochemical and chemical Oxygen demand was (1.19 - 3.08) mg/l (12.4 - 38.01) mg/l respectively while dioxide carbon was ranged(80.5 - 134.2)mg/l.The study revealed that the river water was alkalinity and very hard, as the range of total alkalinity, total hardness, Calcium and magnesium was (131.5 - 227.4, 312.3 - 458.5, 86.4 - 142, 54.8 - 76.8) mg/ respectively, while the concentrations of the nutrients were showed fluctuated in concentrations, it was (102.6 - 558.4, 1.5 - 3.4, ND - 2.9) ?g/ for Nitrate, Nitrite and Phosphate respectively, total organic carbon was between (0.13 - 0.55) %Seven units were diagnosed during the period of study Bacillriophyceae; Chlorophyceae; Cyanophyceae; Euglenophycae; Dinophycae; Chrysophycae ;Cryptophyceae.Species number of phytoplankton that identified through study period were (134, 101, 96) in three sites respectively, and showed that dominance of Bacillriophyceae (diatoms) which was (71, 50, 46) species fallowed by Chlorophyceae (35, 25, 25) species then Cyanophycae (22, 18, 20) species, Euglenophycae (2, 5, 2) species and Dinophycae(2, 3, 1) species Chrysophycae (1, 0, 2) and finally Cryptophyceae (1, 0, 1)species in three sites respectively,. Generally the most common genera in study period are (Nitzschia, Surirella, Nivicula, Navicula, Syndra, Pediastrum, Scenedesmus, Aphanocapsa, Microcystis, Cocconeis, ,).The total number of phytoplankton was low and ranged between (422.21 - 1027.82) cell ?103/l. This study determined means of heavy metals (Cd, Zn, pb, Cu) in dissolved part was (ND - 5, 4.07 - 29.8 ND - 6.89, 2.08 - 8. 71) ?g/l respectively, while particulate part was) 2.09 - 30.16, 44.87 - 372.17, 3.13 - 90.93, 27.41 - 88.20) ?g/g respectively The study revealed also that the concentrations of heavy metals in sedimentswas (ND - 12.71, 11.76 - 39.93, 0.55 - 6.12, 9.31 - 39.73), ?g/g of exchangeable parts and was (1.09 - 17.94, 39.21 - 172.30, 21.21 - 67.96, 33.96 - 76.63) ?g/g of residual parts respectively. The Statistical results showed there are morale differences between locations and seasons in relation to physical and chemical features, organic compounds, heavy metals and phytoplankton. There are negative and positive, morale correlations between phytoplankton and physical, chemical features and heavy metals that measured during study period at probability at the level of (p<0.05).

تطبيق بعض طرق حفظ الحشرات على حشرة المن == Applicating of Some Methods of Maintainance On Aphids

اسم المؤلف: ضياء عباس عبد الرضا الزيادي
اسم المشرف: عبد المحسن حسين مؤنس
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: علوم الحياة
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تعد الحشرات ذات اهمية اقتصادية وطبية وجمالية مما دعت بالباحثين الى دراستها من كافة الجوانب الفسلجية واطوار حياتها وتكيفاتها ومناطق استيطانها وطرق تغذيتها للوصول الى حلول كفيلة للحد من الاضرار المسببة عن الحشرات الاقتصادية والطبية من جانب ولتكثير وتربية ال

دراسة انتشار انواع الفطر Saprolegnia spp. في مياه مدينة الديوانية وتوصيفها جزيئيا == Studying The Spreading of Fungus Saprolegnia SPP. In Waters of Diwaniyah City And Characterization The Molecularly

اسم المؤلف: معتز محمد عزوز الزاملي
اسم المشرف: ماجد كاظم عبود الشبلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: شملت الدراسة الحالية عزل وتشخيص بعض الفطريات المائية ومنها انواع الفطر Saprolegnia spp. في مياه مدينة الديوانية وتوصيفها جزيئيا. عزلت الانواع التابعة لهذا الفطر وهما Saprolegnia parasitica وSaprolegnia ferax من الانهار والبحيرات والاسماك, وتم قياس الخصائ | The present study is concerned with isolation and identification of some aquatic fungi, including species of Saprolegnia spp. in waters of Al - Diwaniyah city and molecular characterization. Two species, Saprolegnia parasitica and Saprolegnia ferax, have been isolated from rivers, lakes and fishes. The physical and chemical environmental characteristics have been measured. The types above have been examined to extract the DNA. The (PCR) technique has been used to examine the presence of protease enzyme. The results of isolation and identification showed that the percentage of the two species were 64% in rivers areas 40% in the lakes areas and 50% in fishes. The rates represented the examined samples which were 60 samples. AS well as isolates was in rivers 16 isolation and in Lakes 10 isolates either in fish was 5 isolation and thus the total isolates 31 isolation, The S. parasitica species is more apparently found during (February, 2014), the number of samples was 12, the less presence of this species in (April, 2014), where the number of sample, was 3. The S. ferax species is more apparently presented in (February, 2014), the number of samples was 10. The less presence was in (April, 2014), the number of samples was 2. The results showed that the number of isolation of Saprolegnia spp. study area as it was S.parasitica species in were 6 in rivers, 2 in lakes, and 3 in fishes. The second species, S. ferax showed number of isolations which were 10 in rivers, 8 in lakes, and 2 in fishes. The PCR examination has used to detect the rDNA gene which responsible for proteases gene and whether it is found in the two species of fungi. The express way has been used to extract DNA and according to the instructions of manufacturer company. The concentrations of DNA in first species were 121.8 - 73.0 mcg/microliter and purity 1.87 - 1.80.In the second species the concentrations of DNA were 2360.4 - 166.9 mcg/microliter and purity 2 - 1.75. The results, by using PCR technique, showed that the percentage of protease in first species was 25% in rivers. In lakes and fish was 100%.Concerning the second species, the percentage of enzyme was 42% in rivers, 40%in lakes, and 50% in fishes. It can be concluded that there is a diversity in fungi species with respect to study areas and enzyme secretion in the stages of fungi infection. The enzyme is more apparently found in S.parasitica species compared with S.ferax species.

دراسة مصلية دمية لداء المقوسات في النساء الحوامل والاطفال حديثي الولادة في محافظة القادسية == Bloody Serological Study of Toxoplasmosis In Pregnant Women And Newborns In Diwaniyah Province

اسم المؤلف: مروة جبار مزهر العبادي
اسم المشرف: خيري عبد الله داود العكيلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية على الاطفال حديثي الولادة في محافظة الديوانية للمدة من بداية شهر تشرين الثاني 2012 الى نهاية شهر نيسان 2013.وقد جمعت عينات الدراسة من دم الحبل السري للاطفال الرضع المولودين حديثا اثناء الولادة في مستشفى الاطفال والولادة التعليمي في | The present study was done on new born babies in Diwania province during a period from the beginning of November 2012 till the end of April 2013. Blood samples were collected from the umbilical cord of the new born babies during delivery in children and maternity teaching hospital. Fifty samples were collected separated to serological tests and for blood pictures. Serological tests were used in this research as follows (Rapid IgG - IgM test, latex test and ELISA IgG, IgM). The results showed the Rapid test was the easiest and cheapest test for diagnosis specific antibodies against Toxoplasmosis, therefore the positive samples were 14% for IgG, 4% for IgM and 36% for overlap cases. While the positive results for latex test was 14% but the highest result was recorded by using ELISA test 30% for IgG, No positive result recorded for IgM. There were variation in positive cases of different ages of mothers, the highest percents found in ages between 30 - 39 years old by using Rapid test 72.72% , while by using The latex test demonstrate high percent of infection was 20% and also for ages between 15 - 19 year old, and the ELISA recorded 50% and the same group of age 15 - 19 years old. The results showed the for the residence factor by using Rapid test showed the rural people were high percent of incidence of Toxoplasmosis 57.14%, but the urban people recorded Latex test and ELISA)17.24%, 31.03%) respectively. Aborted women in previous time were followed by using the serological tests Rapid test 10%, Latex 10% and ELISA zero. The incidence of Toxoplasmosis was high in housewives more than other career, the results were) 34.14%, 34.14 (% for Latex and ELISA, IgM and overlapping cases, while in other career by using Rapid test 66.66% Results showed standard Blood parameters did not register significant differences (P> 0.05) for the total number of white blood cells WBC, hemoglobin Hb, blood volume compact PCV, Erythrocytic RBC, and differential count of white blood cells for Neutrophiles, Lymphocyte, Monocytes, Basophiles and Eosinophiles. Phagocytic activity was prominent in adult people more than newly born babies.

تاثير الثايموكوينون الخافض للسكر والمجدد الانسجة بنكرياس الجرذان المستحث فيها داء السكري باستخدام الستربتوزوتوسين == Antihyperglycemic And Pancreatic Regenerative Effect of Thymoquinone In Streptozotocin Induced Diabetic Male Rats

اسم المؤلف: وجدان ثامر مهدي التميمي
اسم المشرف: جبار عباس احمد الساعدي | هاشم محمد عبد الكريم
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الانسجة
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: To evaluate the anti - hyperglycaemic potent of thymoquinone from Nigella sativa seed in streptozotocin - induced diabetic male rats, the present study has been carried out at the College of Education, Al - Qadisiya University during the period extended from April, 15, 2012 to December, 15, 2012. mRNA expression level of Reg3a, InsI, InsII, PDX1, Pax6, NeuroD1, and MafA genes have been evaluated in pancreatic tissues as well. Sixty five adult male rats (aged 56 days and weighted 138±8.8g) have been used in the present study. Diabetes has been inducted in 52 male rats by injection of single dose of streptozotocin (60 mg/ kg, b.w., i.p.). Diabetes mellitus has been confirmed by blood glucose concentration (to be more than 200 mg/ dl). Intact and streptozotocin - induced diabetic male rats have been assigned to five equal groups (13 per each); Intact (C) and non treated diabetic (DM) rats have been injected with normal saline (100?l, sc) anddrenched with drinking water daily for 42 days. Thymoquinone treated diabetic rats (TQ50 and TQ100) have been injected with normal saline (100?l, sc) and drenched with thymoquinone suspention (50 and 100 mg/ kg, b.w., respectively) daily for 42 days. Insulin treated diabetic rats were injected with insulin (4 IU, sc) and drenched with drinking water daily for 42 days. Body weights were registered daily during the experiment. All overnight fasted animals were sacrificed after general anesthesia by combination of xylazine and ketamine (10 mg and 90 mg/kg, ip, respectively). Blood samples was collected from abdominal vein for determination of serum glucose and insulin concentrations. Samples from pancreatic tissues in all groups have been quickly removed, dipped in liquid nitrogen for RNA extraction and molecular study. Other pancreatic tissues were fixed in formalin forhistopathological and immunohistochemical study. The results demonstrated significant decrease in body weight gain of untreated diabetic (DM) and insulin treated diabetic (DMI) groups as compared with that of intact control (C) and thymoquinone treated diabetic (TQ50 and TQ100) groups, started from the fourth day of experiment, which showed insignificant differences when compared with each other. While the lowest body weight gain has been registered in DM group.Results of serum glucose concentrations referred to significant elevation in diabetic groups compared with intact control. In comparison between the diabetic groups, glucose concentration revealed significant decrease in thymoquinone and insulin treated rats (TQ50, TQ100, and DMI) compared with untreated diabetic rats (DMI). on the other hand, insulin treated males (DMI) and thymoquinone treated males (TQ50 and TQ100) recorded no significant difference in serum insulin concentration when compared witheach other but they were significantly lower than that of intact control male rats (C), but the average means of these four groups were significantly higher than that of non - treated diabetic male rats (DM).Quantification analysis results of gene expression, performed by real - time RT - PCR, revealed that treatment with thymoquinone caused significant increase of mRNA expression levels of Reg3a, InsI, InsII, PDX1, Pax6, NeuroD1 and MafA genes during the studied period. Histological findings of thymoquinone treated pancreases revealed normal cellularity of islets of Langerhans and normal exocrine tissue except few congestion in it, whereas those obtained from non treated diabetic rats showed complet impairment of some islands and highly destructed of others. Normal hepatic architecture with the appearance of radiating shape around the central vein, has been shown in the section obtained from thymoquinone treated diabetic rats except few congestion, obvious regeneration and mitotic division in the nuclei of hepatocytes. Sections obtained from non treated diabetic male rats showed sever congestion, large thrombi in the hepatic tissue, and loss of hepatic architecture with sever hemorrhage, degeneration in hepatocytes, and dilation of sinusoids. Section obtained from kidneys of thymoquinone treated diabetic rats reveales normal renal convoluted tubules with normal epithelium of the tubules and high cellularity of glomeruli. Whearas those obtained non treated diabetic rat revealed dilation of renal convoluted tubules with necrosis in the epithelium of the tubules and sever hemorrhage in the renal tissue. Immunohistochemical results revealed that male rats drenched with thymoquinone registered higher scores of positive cells and intensity of staining compared with other diabetic (DM and I) groups. Hisological sections obtained from pancreases of control male rats showed actively stained islets of Langerhan's by immunohistochemistry with actively stained populations of beta, alpha, and delta cells, whereas those obtained from pancreases of non treated diabetic male rats showed damage of most cell populations and negatively stained for the few remaining beta cells. on the other hand, section obtained from pancreases of diabetic male rats treated with thymoquinone showed actively stained islets of Langerhan's by immunohistochemistry with actively stained populations of beta, alpha, and delta cells. While those obtained from pancreases of diabetic male rats treated with insulin, in the same stages of experiment, showed negatively stained beta cells and other cells of islets of Langerhan's. Histopathological findingsrevealed moderate improvement of pancreatic changes shown in both exocrine and endocrine (Islands of Langerhan's) parts. It can be concluded that drenching of 50 or 100 mg/ kg, bw, of thymoquinone from Nigella sativa seed has potent hypoglycemic effect in experimentally - induced diabetic mature male rats. As well as its positive role in elevating the expression level of Reg3a, InsI, InsII, PDX1, Pax6, NeuroD1

التعبير الكيميائي - النسجي - المناعي والجزيئي لجينات Inh - a وInh - ba وInh - bb في الاعضاء التناسلية لذكور جرذان الوستر البالغة وغير البالغة == Immunohistochemical And Molecular Expression of Inh - A, Inh - Ba And Inh - Bb Genes In Reproductive Organs of Immature And Mature Male Wistar Rats

اسم المؤلف: وداد عبد جواد التميمي
اسم المشرف: جبار عباس احمد الساعدي | عدنان وحيد محمد البديري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الانسجة
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: The present study has been carried out at the department of biology, College of Education, Al - Qadisiya University, Iraq, to investigate the immunological localization of transforming growth factors beta (inhibins and activins) subunits during immature and mature periods and its involvement in male reproductive physiology of rats.At pre - pubertal stage, five male rats of 25, 30, 35, 40, and 45 days old, and at post - pubertal stage, five male rats of 55, 60, 65, 70, and 75 days old have been used in the present study. Experimental animals have been anesthetized and blood samples were obtained from abdominal vein for assesment of activin - A, inhibin - B, FSH, LH, testosterone, and estrogen, testis volumes were measured for each age period. Testes, epididymis, prostate and seminal vesicle, were obtained for molecular and immunohistochemical studyto investigate the expression levels of Inha, Inhba, and Inhbb genes using qRT - PCR and immunohistochemical technique.The present study demonstrated gradual increase of testis volume throughout the male rat life in parallel with the increase of serum inhibin - B and testosterone concentrations. Serum activin - A concentration increased significantly at 30 and 40 day periods. Throughout the post - pubertal stage, activin - A concentration gradually decreased. Serum inhibin - B concentrations gradually decreased at the pre - pubertal stage. Post - pubertal stage registered gradual increase. At 25, 30, and 35 day periods, serum FSH level registered no significant changes, whereas 40 day period recorded significant increase then decreased at 45 day period. Throughout the post - pubertal period, the level of FSH concentrations continued in gradual decrease. At 25, 30, 35, and 40 day periods, serum LH and testosterone levels showed no significant differences, whereas 45 day period recorded significant increase. Postpubertal period showed gradual significant increase. Serum estradiol concentration decreased gradually at the pre - pubertal stage and continued in decrement at the post - pubertal stage.The expression level of Inha gene in testis decreased as the age progress until 40 day period, and then slightly increased at 45 day period. At 55 day period, the expression significantly increased. At 60 and 65 periods, the levels recorded no increase, but 70 and 75 day periods recorded significant increase. The expression level of Inhba gene increased significantly as the age progress at the pre - pubertal stage, where the highest level was recorded at 45 day. At 55, 60, and 65 day periods, the highest expression level has been recorded, thenafter, the levels decreased at 70 and 75 day periods. The expression level of Inhbb gene increased significantly at 30, 35, 40, and 45 day periods of the pre - pubertal stage. At 55 and 60 day periods, no significant difference was recorded compared with 45 day period. At 65 day period, the highest level was recorded, thenafter, the levels decreased at 70 and 75 day periods. The expression level of Inha gene in epididymis recorded no significant difference at all periods of the pre - pubertal stage, but the post - pubertal stage showed gradual significant increase as age progressed. The expression level of Inhba gene recorded no difference at 25, 30, 35, and 40 day periods, but it was significantly higher at 45 day period. The levels at 55, 60, and 65 day periods recorded no significant difference when compared with each other or with 45 day period. Significant increase has been recorded at 70 and 75 day periods. The expression level of Inhbb gene increased significantly at 30 and 35 but it decreased at 40 day period, then it showed further increase at 45 day period. At 55, 60, 65, and 70 day periods, also increased but the highest expression level was recorded at 75 day period.The expression level of Inha, Inhba, Inhbb genes in prostate recorded no significant difference at all periods of the pre - pubertal stage, but the postpubertal stage showed significant increase at all periods.The expression level of Inha and Inhba genes in seminal vesicle recorded no significant difference at 25, 30, 35, and 40 day periods and significant increase at 45 day period. The 55, 60, and 65 day periods recorded no significant difference when compared with each other but significant increase has been recorded at 70 and 75 day periods. The expression level of Inhbb gene recorded no significant difference at 25, 30, and 35 day periods, but it increased as the age progressed, whereas the levels showed no significant difference at all periods of the post - pubertal stage, but they were significantly higher than that recorded at the pre - pubertal stage. The results of immunohistochemical study demonstrated positive immunostaining for inhibin - ? subunit in Sertoli cells and primary spermatocyte and no staining in Leydig cells of pre - pubertal rat testis, whereas post - pubertal testis showed positive immunostaining in Sertoli cells, Leydig cells and primary spermatocyte at 55 day period and positive immunostaining in Sertoli cells, Leydig cells, and spermatogonia at 60 day period. There was positive immunostaining in Sertoli cells, Leydig cells, spermatogonia, primary spermatocyte and spermatid at 65, 70, and 75 day periods. At 25 and 30 day periods of pre - pubertal stage, rat epididymis showed moderate positive immunostaining in epithelial cells, but strong positive staining in epithelial cells has been shown at 35, 40, and 45 day periods and at all periods of the post - pubertal stages. The result expressed positive immunostaining in the epithelial cells of the pre - pubertal rat prostate and strong positive immunostaining at the post - pubertal stage. on the otherhand both, pre - pubertal and post - pubertal rat seminal vesicle showed strong positive immunostaining in epithelial cells. Weak positive immunostaining of Inhba has been observed in the primary spermatocyte and no staining in Sertoli cells and Leydig cells in the prepubertal rat testis and at 55 day period of the post - pubertal rat testis, and no staining in Sertoli cells, Leydig cells and spermatogenic cells at 60, 65, 70, and 75 day periods. Moreover there was positive immunostaining in epithelial cells in both the pre - pubertal and post - pubertal rat epididymis and prostate. In the post - pubertal stage, rat prostate showed strong positive mmunostaining in epithelial cells. No staining was observed in epithelial cells in both the prepubertal and post - pubertal rat seminal vesicle. The pre - pubertal rat testis showed strong immunostaining for Inhbb subunit in Sertoli cells, Leydig cells and primary spermatocyte, in adittion to spermatid in post - pubertal rat testis. Strong positive immunostaining in epithelial cells of the pre - pubertal rat epididymis at 25 and 30 day periods was expressed, but moderate positive immunostaining in epithelial cells of prepubertalrat epididymis was observed at 35, 40, and 45 day periods and postpubertal rat epididymis. However Inhbb subunit in both pre - pubertal and postpubertal rat prostate showed positive immunostaining in epithelial cells. There was positive immunostaining in epithelial cells in the pre - pubertal rat seminal vesicle at 25, 30, 35, and 40 day periods, and strong positive immunostaining at 45 day period and post - pubertal stage.It could be concluded that serum inhibin - B has positive correlation with testis volum and testosterone concentration at pre - and post - pubertal stages, and positive correlation with FSH and LH concentrations at pre - pubertal but negative partial correlation at post - pubertal stage. There was relationship between serum inhibin and activin concentration and fold changes of Inha, Inhba, and Inhbb genes in testis, epididymis, prostate, and seminal vesicle tissues at all periods of the study. Positive immunostaining for inhibin ? - and ?B - subunits, but not for ?A - subunit has been shown in testis cells and epithelial cells of seminal vesicle, positive immunostaining for inhibin ?, ?A, and ?B subunits in the epididymis and prostate

تقييم خليط الكيتامين والترامادول والباراسيتامول والزايلازين كبرامج للتخدير العام بالحقن في الاغنام == Evaluating of Ketamine, Tramadol, Paracetamol And Xylazine Combination As An Injectable General Anesthetic Protocols In Sheep

اسم المؤلف: علي اسماعيل جاسم
اسم المشرف: ثاير علوان عبد
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الجراحة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: هدفت الدراسة الى تقييم استخدام الكيتامين (K)، والترامادول (T)، والباراسيتامول (P) والزيلازين (X) كبرامج تخدير للاغنام باستخدام جرعات مختلفة وطرق اعطاء مختلفة. استخدم في التجربة ثلاثون من الاغنام الكبار البالغة من السلالة المحلية (تراوحت معدل اوزانها 27.4 | The study aim to evaluate the use of ketamine (K), tramadol (T), paracetamol (P) and xylazine (X) as an anesthetic protocol for anesthesia in sheep by using different doses and different routes of administration. Thirty animals of adult local breed sheep weighing (27.4±2.46) kg were used in 6 different anesthetic protocols. The animals were divided into six groups (5 animals of each). The first three groups (without using of xylazine) were giving the drugs by IV injection in the jugular vein : G1 giving K4 and P10 mg/kg IV, G2 giving K4T2 mg/kg IV, and G3 giving K4 T2 P10 IV. In the other groups (G4, G5, and G6) xylazine were adding to the anesthetic protocol. G4 giving K4 T2 P10 X0.05 by IV route. G5 was giving K10 T2 P10 X1 mg/kg by IM route and G6 giving K20 T4 P10 X2.5 mg/kg by IM route. The HR, RR, RT, muscle relaxation, limb, and flank analgesia, and rumen movement were taken before administration of anesthesia (time 0) and consider as the control reading. Then taken at 5, 10, 15, 20, 30, 45 and 60 minutes or till the end of anesthesia. In the IV groups (without using of xylazine), (G1, G2, and G3). The induction time was 53.2, 60, and 68 seconds, the surgical anesthesia was 2.8, 5, and 5 minutes, and the total recovery time was 4.8, 5.4, and 3 minutes in G1, G2 and G3 respectivily. The RR, and HR were increased, and the RT was decreased. The muscle relaxation was mild, and the analgesia was moderate. In G4 after adding of xylazine and giving the anesthetic protocol by IV route, the induction time, the surgical anesthesia, and the total recovery time were become 50.6 seconds, 23 minutes, and 9.4 minutes respectively. The HR, RR, and RT were increased, the muscle relaxation, and analgesia were moderate. In G5 after increased the dose of ketamine and xylazine and giving the anesthetic protocol by IM route, the induction time, the surgical anesthesia, and the total recovery time were 5, 36, and 5.6 minutes respectively. The HR and RR were decreased, and the RT was increased. The muscle relaxation was deep for 10 minutes, and the analgesia was moderate for 10 minutes also. In G6 after doubling the ketamine, tramadol, and xylazine doses, and giving the anesthetic protocol by IM route. The induction time, the surgical anesthesia, and the total recovery time were 3.6, 73.4, and 7 minutes respectively. The HR started stable, then after 15 minutes decreased sharply, The RR was irregular, with apnea, the RT was increased, the muscle relaxation was deep for 35 minutes, and the analgesia was deep for 45 minutes. In conclusion the addition of tramadol and paracetamol to the ketamine anesthesia of sheep in low doses, improve the induction and recovery time of anesthesia, without increase the duration and depth of anesthesia. Adding of xylazine to the anesthetic protocols increase the duration and depth of anesthesia. The protocol of K10 T2 P10 X1 by the IM route is the best protocol dose among the six protocols used in this study.

Coccidiosis In Poultry

اسم المؤلف: يثرب خضر عبيس
اسم المشرف: نافع صبيح جاسم
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الامراض - الدواجن
الدرجة: دبلوم عالي
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تعتبر الكوكسيديا في الدواجن واحدة من الامراض الرئيسية التي تؤثر على اداء الدواجن المرباة تحت نظام الانتاج المكثف الذي يؤثر على صناعة الدواجن في جميع انحاء العالم، بخسائر اقتصادية كبيرة عن طريق قلة التحويل الغذائي وخفض الانتاجية. هذا ليس فقط يعيق نمو الدجا | Coccidiosis in poultry is still considered as one of the main diseases affecting performance of poultry reared under intensive production system that affects the poultry industry worldwide , having major economic losses in poultry by reducing performance and decreasing productivity.this disease not only hinders the growth of chickens but also facilitates other epidemic diseases. Coccidiosis is mainly controld by prophylactic coccidioststs administrated in the feed. However , the extensive use of these drugs has resulted in the development of drug resistance by Eimeria spp., Which causes Coccidiosis.The aim of the study was to updated the recent information of of infection,gross evaluation of the intestinal tract and microscopic evaluation of wet smear are used as routine diagnostic methods and to acquire data on the prevalence of coccidiosis and drug resistance of field isolated in chickens,Frequent use of anticoccidial drugs ,however , has resulted in the development of resistance in the Eimerian spp. Because farmers mainly rely on the prophylactic and therapeutic use of chemicals for the control of avian coccidiosis. Increasing awareness about populic health hazards associated with drug residues in food chain has also added to the constraints in using synthetic drugs for treatment and control of disease in animals and control methods according to the collected formation chickens were in danger of clinical coccidiosis , and here coccidia generated a certine degree of resistance and not easy ty control in our industry in Iraq because of that the poultry industry in Iraq is not as integrated system without strict biosecurity. The study also highlight the information on the anticoccidial vaccines are in the market with controversial efficacy.

تشخيص فايروس التهاب القصبات المعدي في حقول دجاج اللحم في محافظة ذي قار باستخدام تقنية الاليزا (ELISA) وتفاعل سلسلة البلمرة المنعكس في الوقت الحقيقي (rRT - PCR) == Identification of Infectious Bronchitis Virus In Thi - Qar Province In Broilers Farms By Using ELISA and rRT - PCR

اسم المؤلف: ظافر ياسر عزيز الفاضلي
اسم المشرف: نافع صبيح جاسم
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الامراض - الدواجن
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة لتقييم مدى انتشار مرض التهاب القصبات المعدي الفيروسي في دجاج اللحم في محافظة ذي قار, قسمت منطقة الدراسة جغرافيا الى خمسة مناطق (شمالية, جنوبية, غربية , شرقية, مركزية) حيث تمت الدراسة على عشرون حقل دجاج لحم غير ملقحة اربعة قاعات من كل منط | The current study was conducted for evaluation of prevalence of IB in broilers in the Thi - Qar province, which geographically divided into five regions, (Northern, Southern, Western, Eastern and central area), twenty broiler farms without history of previous vaccination, four from each region were included in this study. Serum and autopsies were collected from affected birds after recording of the clinical signs that observed on the birds; positive serum samples that detected by rapid test were used for evaluation the antibodies titrations by ELISA technique, while the histological specimens (trachea, lung and kidney) were used for detection of histopathological changes and detection of presence of the IBV infection by molecular approach (real time - PCR). The clinical manifestation includes : typical respiratory sings of IB infections, with depression, rough feather, and assembling near the heat source, as well as some birds were found dead with mortality rate ranging between 30 - 80%. Also 96 serum samples were collected from other broiler farms as following ( 42 asymptomatic vaccinated, 54 asymptomatic non - vaccinated, 26 symptomatic vaccinated) for ELISA and real time PCR tests. The result of rapid test showed that the percentages were different among the areas of study when the southern area revealed the highest percentage (80%) among the others followed by the northern area and western (70%), then central and eastern(65%). The results of ELISA showed a high titer for antibody of IBV in all these farms in areas of the study, but the highly significant results (P<0.05) were found in the southern area(7032.2±1640.92) and then northern area (5722.18±2060.1), a less significant differences were in central and eastern areas (3188.11±742.45; 3161.8±52.6) respectively. In relation to age, the high titer of IB antibodies in total 20 farm by ELISA were recorded in 12 farms at 4 weeks of age (60%) followed by 4 farms at 5 weeks of age (20%), 3 farms at 3 weeks of age (15%) and 1 farm at 2 weeks of age (5%), while there is no recorded infection in farms at 6 ages. According to history of vaccination and clinical manifestation of tested birds, most of the tested flocks showed high level of antibody titers to IBV by ELISA technique in asymptomatic vaccinated chickens samples 38/42 (90%) followed by asymptomatic non vaccinated chickens 8 (14.8%) and symptomatic vaccinated chickens 6(23.07%). While rRT - PCR showed that 69 ( 80%) sample were revealed positive results. The most important gross lesion observed on immediately the necropsy after death were tracheal congestion, caseous exudates in the trachea, pneumonia, airsacculitis, pericarditis and perihepatitis, as well as, nephritis; while The histopathological changes were summarized by desquamation of tracheal epithelial cells and hemorrhage with infiltration of inflammatory cells specially lymphocyte cells, hyperplasia of epithelial cells with hemorrhage, lung sections showed severe infiltration of inflammatory cells mostly lymphocyte cells and severe congestion with spots of pneumonia and the histopathological changes in kidneys were characterized by tubules degeneration , glomerular distention with severe hemorrhage and lymphocyte infiltration.
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