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الاضطرابات الفسلجية والاجهاد التاكسدي لدى النساء في سن بعد الياس في محافظة بابل == Physiological Disturbances And Oxidative Stress In Postmenopausal Women In Babylon Province

اسم المؤلف: سارة حاتم عجيل خضير

اسم المشرف: داخل غاني عمران الوطيفي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الحيوان

الدرجة: ماجستير

الجامعة: جامعة بابل - كلية العلوم للبنات - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: بابل

الصفحات الاولى:

المستخلص: يتميز سن الياس (Menopause) لدى الاناث بتوقف الحيض وتصبح الانثى غير خصبة بسبب فقدان جميع الحويصلات المبيضية (Ovarian follicles) والانخفاض الشديد لهرموني الاستروجين والبروجسترون صممت الدراسة الحالية لفحص الاضطرابات الفسلجية المختلفة والتي تشمل بعض المعايير | Menopause marks the time in women's life when her menstruation stop and she is no longer fertile because of exhaustion of ovarian follicles and sharply decrease in ovarian production of estrogen and progesterone.The present study was designated to determinate different physiological disturbances which occurring in some hematological and biochemical parameters of postmenopausal women. A total number used was 130 women, 100 women were postmenopause and subdivided into four age groups (46 - 50 year, 51 - 55 year, 56 - 60 year, and 61 - 65 year). Thirteen women were premenopause , used as a control group, their ages ranged between 20 - 30 years. In all age groups of postmenopausal women, it have been found that the levels of estradiol hormone were progressioly decreased (p<0.05) when compared with control group. Regarding hematological parameters, RBCs count was insignificantly increase (p>0.05) in all age groups of postmenopausal women. Levels of hemoglobin concentrations and packed cell volume were significantly increased (p<0.05) in all age groups of postmenopausal women compare to control groups. Blood platelets count recorded insignificant fall (p>0.05) in most age groups of postmenopausal women.As for, red blood indices (MCV, MCH, and MCHC) explained insignificant differences (p>0.05) of most menopausal women in a comparison with those control group. Total white blood cells have insignificant elevation (p>0.05) of most groups except 56 - 60 years groups. Regard to differential white blood cells, Levels of lymphocytes and monocyte have been showed insignificant drop (p>0.05) of the level of lymphocytes and significant increase (p<0.05) of first and second age groups in the levels of monocytes of postmenopausal women. Granulocytes white blood cells have been indicated different changes : Nentrophils were significantly higher (p<0.05)in all age groups of postmenopausal women, where as, eosinophils and basophils were insignificantly different (p>0.05) in most age groups. Concerning biochemical changes, Total serum proteins, Albumin, and globulin have been showed significant lowering (p<0.05) in all age groups of postmenopausal women. Statistical analyses showed a significant rise (p<0.05) of serum uric acid and urea levels in most group of postmenopausal women. Measurments of enzyme activities involved in liver (ALT and AST) were significantly hightened (p<0.05) in most age groups of postmenopausal women, Whil, ALP activities recorded insignificant increase (p>0.05) in all age group of postmenopausal women. Determinations of minerals showing a significant increase (p<0.05) in the levels of calcium and insignificant increase (p>0.05) in the levels of phosphorus in all groups of post menopausal women. Analyses of electrolytes have been explained a significant elevation (p<0.05) of the sodium and non significant difference (p>0.05) of the potassium in all groups of postmenopausal women.The present study involved determination specific and essential trace elements, Levels of copper explained a significant decrease (p<0.05) in all age groups associated with a significant increase (p<0.05) of zinc levels in postmenopausal women. Inversly, Values of magnesium were insignificantly different (p>0.05) in all age groups of postmenopausal women. The present study selects specific parameters to explaine oxidative stress in postmenopausal women. Levels of malondeheyds were significantly elevated (p<0.05) in all groups of postmenopausal women, and correlated negatively (r= - 0.102) with level of estrogen. Values of reduced glutathione showed a significant decrease (0.05) in postmenopausal women and correlated positively (r=0.64) with the estrogen hormone. Analyses of antioxidants enzymes have been showed a significant decrease (p<0.05) in the activity of catalase enzymes of all groups of postmenopause. Also, the activity of glutathione peroxidase was significantly decrease (p<0.05) in postmenopausal women and its correlation coefficient was r=0.263. In conclusion, changes summarized above may be attributed to deficiency of estradiol hormone in postmenopausal women which resulting in different physiological disturbances and these changes become aggravated and exacerbated because of oxidative stress occurring in decrease of estradiol hormones.

دراسة انتشار الحيوانات الابتدائية الرئيسية المسببة للاسهال بين المرضى باستخدام الطرائق المجهرية والجزيئية في محافظة بابل == Prevalence Study For Main Protozoa Diarrheal Agents Among Patients By Using Microscopically And Molecular Methods In Babylon Province

اسم المؤلف: ميس كاظم عليوي

اسم المشرف: احمد خضير عبيس الحميري

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: علوم الحياة

الدرجة: ماجستير

الجامعة: جامعة بابل - كلية العلوم للبنات - قسم علوم الحياة

اللغة: الانكليزية

مكان الجامعة: بابل

الصفحات الاولى:

المستخلص: اجريت الدراسة الحالية خلال المدة من تشرين الاول 2014 الى شهر شباط 2015 حيث تضمنت هذه الدراسة فحص 987 عينة براز (اطفال وبالغين, ذكورو اناث) بطريقة الفحص المجهري المباشر و96 عينة موجبة فحصت عن طريق تقنية تفاعل سلسلة متعدد البلمرة. للمرضى المصابين بالاسهال و| The current study during period was conducted from October 2014 till February 2015 examination of 987 stool samples for direct smear method (Lugol's Iodine, Normal Saline (0.9%) for detection of the following parasites G.lamblia, E.histolytica while using floatation methods and Ziehl - Neelsen method (Malachite green) for detecting the Cryptosporidium spp. by using light microscope) and 96 positive samples from these samples examined by polymerase chain reaction technique PCR. For patients infected with diarrhea (children and adults, male and female) who attended to Babylon maternity and children hospital and specialized Marjan Hospital for Internal and Cardiac Diseases in the Babylon province as well as primary health care and private clinics. The age ranges from(Less than one year - 31and more).The current study showed the rate of infection with parasites that causative of diarrhea47.3% (E.histolytica, G.lamblia and Cryptosporidium spp.) was 26.4%, 17.9% and 3.7%, respectively.They were examined by a direct smear method to detect the trophozoites, cyst and oocyst phases of these parasites. The highest rate of infection by microscopic examination was in the rural area 67.2% in comparing with city that was 32.9%. Also the highest rate of infection among males was 51.5% in comparing with females 41.2%. It has been recorded that the higher rate of infection was 76.1% in the age group (16 - 20) years while the lower infection rate was in the age group (26 - 30) years that was 22.8%. It has been observed significant differences in infection rates at the (P ? 0.05). The present study recorded difference in the rates of parasitic infections according to the presence of animals in houses, the high rates of infection where with those have animals in their houses 51.2% while the lowest rates of infection where with those not have animals in their houses 38.9%.It has been observed significant differences in infection rates at the (P ? 0.05). This study reveals a difference in parasitic infections rates, according to the level of education the head of household, and found that the highest rate of infection for those with the head of the family is non - educated or illiterate 48.3%, while the rate of infection was declined with head of the family who get primary education level 46.2%. It had recorded the highest infection rate in October 63.8%, while the lowest percentage was in February 27. 6%. It has been observed significant differences in infection rates at the (P ? 0.05). In the present study Polymerase chain reaction (PCR) is used to 96 positive results in direct smear methods to detection the main parasitic diarrhea agent. It had recorded total of infection rate of 43.4% (31.3%, 28.1% and 2.1%, respectively). Depending on PCR technique showed the highest rates of infection was in the male 36.7% while the lowest rates of infection in females 30.6%. It had recorded the highest rate of infection in the rural areas 45.3% in comparing rate of infection in the urban areas 25.9%. The present study recorded the highest rates of infection were in the (16 - 20years) age group 46.2%, while the decline in the rates of infection was in the (21 - 25years) age group 16.7%. Also it showed the highest rates of infection were in the presence of animals in the houses 36.1% while the lowest rate of infection where there were no animals 31.4%.It has been observed significant differences in infection rates at the (P ? 0.05). The study found that the highest rates of infection for those with the head of the family is non - educated or illiterate 38.9%, while the rate of infection declined with head of the family who got Academic education level 33.3%. Also it had recorded the highest rate of infection in November (2014) 42.1%, while the lowest rates percentage was in December (2014) 23.1%. It has been observed significant differences in infection rates at the (P ? 0.05). Through the current study it was concluded that the prevalence of the parasites that cause diarrhea in the Babylon province are very highly when detection microscopic examination and PCR method, comparison with previous studies and rural area highest rates of infection from urban area.

التوصيف المظهري والجزيئي لبعض الفطريات الخيطيه والخمائر المعزولة من اخماج العين واختبار حساسيتها الدوائيه تجاه بعض المضادات الفطرية == Morphological And Molecular Identification of Some Filamentous Fungi And Yeast Isolated From Eye Infections And Theirs Susceptibilities Toward Some Antifungals

اسم المؤلف: يسر فاضل عبد الامير الاسدي

اسم المشرف: زيدان خليف عمران المعموري

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة بابل - كلية العلوم للبنات - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: بابل

الصفحات الاولى:

المستخلص: تمت هذه الدراسة في مختبر التقنيات الاحيائية التابع لكلية علوم البنات / جامعة بابل للفترة من اكتوبر 2012 الى اكتوبر 2013، حيث تم جمع 165 عينة سريرية من المرضى المراجعين لاستشارية العيون في مستشفى الحسين العام ومن بعض العيادات التخصصية لاطباء العيون في محاف | The study was performed in Biotechnical Laboratory in Science collage for women, Babylon University from Oct.2012 to Oct.2013. 165 clinical samples were collected from patients with eyes infections were admitted to AL - Hussein main hospital in Karbala province and private clinics of eye physician, after diagnosis of eye infection by specialize physician the clinical samples collected by using swab from Cornea, Conjunctiva, Lacrimal sac, eye allergic, contact lenses and from pull water eye after surgery, the samples included different sex & age groups.All clinical samples culturing on SDA and PDA, Yeast was identified base on their color reactions on Chromo agar Other biochemical test were performed.Antifungal sensitivity was performed against 8 antifungal (Nystatin, Econazol, Amphotericine B, Flucosytosine, Miconazole Metronidazole, Griseofulvin, Ketoconazol, Most fungi showed variable degrees in their susceptibilities to the antifungal agents. In this study we diagnostic (57) isolate from filamentous fungal species, Aspergillus spp. and Fusarium spp. showed highly frequent in ratio 20.1% and 9.6, In addition (37) yeast isolated, C.albicans and C.famata representative the highest frequent isolates.Molecular identification and genotyping, 8 isolates identified as C.albicans from 24 of selective Candida isolates were performed by specific pair primer (CABC) that give genotype with 515 base pair, genotype ITS1/ITS4 region show polymorphic PCR product help in diagnosis other (7) Candida species like C.famata, C.rugosa, C.glabrat, C.utili, C.saitoana, C.guilliermondii, C.inconpicua. The pair primer for amplified the transposable intron (CA - INT) was succeed in amplification of intron showed two genotypes : type A (450bp) and type B(850bp) and showed there was no C.dubliniensis isolates in our Candida isolate, This result was coincidence typing results between specific pair primer (CABC) and Microsatellite CAI for C.albicans.The amplified 18s region by using NS/EF3 and the result give genotype with molecular weight 1700bp.Also the study include using RFLP technique & the digested by using CfoI enzyme & the results for ITS PCR products of 24 isolates of Candida showed unique RFLP patterns.