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دراسة التاثيرات الحادة والمزمنة للمبيد العشبي - 4, 2 دايكلوروفينوكسي اسيتك اسد في نوعين من اسماك الكارب == Study The Acute And Chronic Effects of The Herbicide 2, 4 - Dichlorophenoxy Acetic Acid In Two Species of Carp Fish

اسم المؤلف: ضحى زكي السويفي
اسم المشرف: احمد جاسم محمد العزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية التاثيرات السمية الحادة والمزمنة لمبيد 2, 4 - d في نوعين من اسماك الكارب (الاعتيادي والعشبي)، استعمل في دراسة التاثيرات الحادة عدة لكلا النوعين قيد الدراسة وكانت ((170, 165, 160, 155, 145, 140, 130 و(140, 130, 115, 95, 70)ملغم/لتر ع | This study included acute and chronic effects of 2, 4 - d pesticide in two species of carp fish (Cyprinus carpio and Ctenopharyngodon idella) using several concentrations for the two carp fish species that were (130, 140, 145, 155, 160, 165, 170) and (70, 95, 115, 130, 140) mg/l respectively for 96 hours, to determine the median lethal concentration (LC50) and the behavioral changes was used as a standard of those effects. Whereas three concentrations (20, 40 and 60) and (10, 15, 25) mg/l respectively for the two carp species were used in the chronic exposure for six weeks. The behavioral, histological and hematological changes were used as a standard to observe these effects through the study period. The value of LC50 for common carp fish were 157.77, 157.25, 156.41 and 152.62 mg/l. For grass carp, LC50 were 115.92, 114.92, 112.94 and 96.52 mg/l for 24, 48, 72, 96 hrs of exposure to 2, 4 - d pesticide concentrations respectively.Behavioral changes were recorded more effects in high concentrations than in low concentrations. To discover the toxic effects of this substance on fish, many parameters were used. They included the clinical symptoms by erratic swimming, hyperactivity, loss of equilibrium with respiratory effects such as rapid gill movement and swam near the water surface. While in chronic effects, blood parameters were changed in Red Blood Cell R.B.C., White Blood Cell W.B.C., Packed Cell Volume P.C.V., Haemoglobin Hb and liver enzyme (GPT & GOT).Values elevated significantly according to the pesticide concentration (P?0.001) and exposure periods (P?0.05) in comparison with control. The histological examination of the gills, liver and kidney of the common and grass carp fish after chronic exposure to 2, 4 - d herbicide showed pathological changes and alterations such as fusion of the secondary lamella, epithelial lifting, blood congestion, epithelial hypertrophy of the lamellar epithelium and epithelial necrosis. In the liver, pathological changes were seen, including hepatocytes hypertrophy, cytoplasmic vacuolation, blood congestion and cellular necrosis. Degenerative changes of the renal epithelium, necrosis in renal tubules and occlusion of the tubular lumen where the pathological alterations in the kidney.

تاثير الماء المعالج مغناطيسيا في بعض الصفات المظهرية والكيموحيوية في عقل نبات الماش (Phaseolus aureus Roxb.) == Effect of Magnetically Treated Water On Some Morphological And Biochemical Properties of Mung Bean (Phaseolus Aureus Roxb.) Cuttings

اسم المؤلف: عامر راضي عبد الحسين جبار
اسم المشرف: بشير عبد الحمزة محمد العلواني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: This study was conducted in order to investigate the role of magnetically water in the rooting of fresh and aged (phaseolus aureus roxb.) cuttings, and the impact of treated water in some morphological criteria and biochemical that interfere in the induction of rooting in cuttings and fall clearly when delayed induced auxin treatment in aged cuttings. Study was conducted in the laboratories of the Department of biology at the University of Babylon, for the period 2012 - 2014 was the use of magnetic therapy devices (Magnitron) of water with three gauss 1000, 2000.3000 so as to provide a magnetic flow to distilled water and river water and used as well as the complete randomized design and analyzed the results statistically significant and valuable knowledge LSD to extract the significantly differences between the treatments and the results were as follows : Physiological parts1. Increase the number of roots significantly in cuttings when treat for 24 h.with auxin dissolved with distilled water processor in 1000 gauss amounted to 77.75 root / cutting.2. The results showed that the roots rate increased significantly in the treatment of fresh cuttings treated in distilled water processor 1000 gauss gave 64 root / cutting. As well as the rest treatments affect in rooting increase as given (58, 52.25) root / cutting for each of the treatment 2000.3000 gauss, respectively.3. The treatment of river water as well as magnetically influenced in increasing the number of roots significantly in three processes and the highest being in the 1000 gauss(84) root / cutting.4. The results showed that distilled water processor magnetic effect is significant in the processes that occur during ageing of the cuttings, which led to a reduction in rooting rate in aged cuttings, as it was observed that the treatment of the cuttings for 72 - hour in 1000 gauss is the best of the rest of the treatments amounted to 48 root / cutting. As the impact of river water processor magnetically significantly increase the number of roots in the aged cuttings as treatment given to 1000 gauss higher values 70 root / cuting.Biochemical parts.1. The results showed that chlorophyll content increased significantly when using the river water wizard three intensities (18.09, 17.43, 16.99) (spad) intensities of 1000, 2000.3000 gauss respectively.other each of distilled water and river water processor magnetically in 1000 gauss increase in chlorophyll content to 16.07, 18.57 (spad) respectively in aged cuttings.2. It was observed that the treatment of auxin dissolved in three intensities impacted significantly on the effectiveness of antioxidants defense system and the highest being at the treatment of the cuttings in auxin dissolved water magnetically processor in 1000 gauss, as was the effectiveness of the enzyme SOD 1.85 unit in the effectiveness of the enzyme CAT reached 15.30 unit and increased GSH content to 0.914 mg / g when compared with the control treatment.3. influenced the treatment of fresh cuttings in distilled water magnetically processor with three intensities are equally matched in the effectiveness of SOD and CAT, and the highest being at 1000 gauss magnetic intensity as it affected the effectiveness of the CAT to 31.62 units. Magnetic intensity also affected the same increase in GSH content reached significantly to 0.710 mg / g.4. The results showed the treatment of fresh cuttings river water magnetically wizard in three intensities increase in the effectiveness of the enzyme SOD and CAT, and the highest being at the treatment of 1000 gauss, amounting to 4.168, 45.9 U for both enzymes, respectively, as GSH content increased significantly, reaching 0.810 mg / g in the treatment of the fresh cuttings in 1000 gauss.5. I have not noticed any significant effect of distilled water processor magnetically as well as river water in the MDA content.6. The impact of distilled water processor 1000 gauss significantly in increasing the effectiveness of the enzyme SOD and CAT in aged cuttings, amounting to 2.88, 42.84 units respectively, as GSH content increased to 0.785 mg / g when the treatment of tresh cuttings in distilled water processor with 1000 gauss and also affected the rest of the intensities increase in GSH content in aged cuttings.7. increased the effectiveness of enzyme SOD and CAT when treated of aged cuttings in river water processor magnetically 1000 gauss, amounting to 2.9, 51.2 units respectively, as GSHwas significant increase in content to 0.854 mg / g when treatment aged cuttings in river water processor 1000 gauss and also affected the rest of the intensities increase in GSH content.8. The use of treated water magnetically 1000 gauss significantly effect in reducing the MDA content in aged cuttings 0.293 mg/g fresh weight.

التحري الجزيئي عن بعض التغيرات في الدنا المايتوكونديري للنطف لمرضى يعانون من وهن حركة النطف == Molecular Screening of Some Changes In Sperm Mitochondrial DNA In Asthenozoospermic Patients

اسم المؤلف: عدي عدنان مهدي
اسم المشرف: اسماعيل عبد الرضا عبد الحسن
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: من خلال البحوث والدراسات التي اجريت على اسباب وهن حركة النطف لدى الرجال الا انه هناك عدد من المسببات لم تحدد والى الوقت الحاضر. الا انه بالرغم من ذلك بعض الدراسات تشيربان العامل الوراثي يلعب دور في ذلك والمتمثل بعضيات المايتوكوندريا والحاوية على الدنا الم | Through researches and studies done on the causes of the asthenozoospermia in men, although there was no specific reason so far responsible for the infertility in people, but some of the studies were indicated that the genetic factors plays a role in the sperm dysmotility through the mitochondria that contained mitochondrial DNA responsible for providing the energy required for the sperm motility by production of ATP, which serve as the fuel that is consumed by sperm during motility. This study is conducted to determine the changes that could occur in the sperm mtDNA which included (common deletion, mtDNA copy number per cell and mutations in the ATPase8 and ATPase6 genes).This study was consisted of two parts as follow : The first part was consisted of two steps : The first step conducted on 71 samples from subjects suffering from the weakness of the movement of sperm and 12 samples from subjects who have a normal movement of sperm, total samples were 83 samples were divided patient samples into 5 groups depending on the percentage of the sperm motility under the classification adopted by the World Health Organization as well as a control group, that it has become 6 groups, these samples were subjected for investigation about the mitochondrial DNA deletions and mitochondrial DNA copy number by using real time PCR.On the other hand was taking 66 sample dispersed sample 56 patients and 10 healthy samples were subjected to the tests of ATPase6 and ATPase8 genes sequences for detection about mtDNA mutation.The second step was included the use of discontinuous gradient centrifugation (percoll) , which are 40 % and 80 % which represent both progressive and non progressive motile sperms, bringing the total samples 166 samples that collected and fractionated in a hospital, Kamal Al - Samarrai to treat infertility and IVF in Baghdad and examined for further molecular investigations for the period from February 2012 to October 2013.The second part was included the molecular tests that carried out after DNA extraction from the cells and this part include three steps : The first step was to investigate the common deletion in mtDNA where the results showed that the total number of deleted samples in the group (0 - 5) in the nonprogressive sperm was 81.25 %, which ranged between 6.8 - 74.7% while the ratio between the other groups 6 - 10, 11 - 15 , 16 - 20, 21 - 25 where were 75, 35.72, 28.5 , 36.3, and 12% for control group respectively. The second step was to investigate the sperm mtDNA copy number. The same groups where the results showed that the content of DNA in subjects with impaired movement of sperm was high compared with the control group where scored in some samples the highest level of gene expression 246.9 copies per cell in the group (0 - 5%) either other groups have shown lower levels were observed in groups (6 - 10) , (11 - 15) , (16 - 20) and (21 - 25) were 203.1, 180.7, 133.5 and 128.3 copies per cell, either the highest level of DNA content of the control group was 94.7 copies per cell, there was also a significant difference (P>0.05) between the normospermic motile isolated from 80% class and poor sperm movement isolated from 40 % class. The third step was included the detection of mutations by analyzing the sequences of the MT - ATAase8 and MT - ATPase6 genes which documented 59 mutations that were 23 missense and 36 silent mutations where all of them were heteroplasmic mutation except for a single mutation of the type missense (A8860G) which was homoplasmic mutation was noticed in all mtDNA copies of patients and control subjects also in progressive and non progressive sperm cells. Thought this current study novel nucleotides changes in MT - ATAase8 and MT - ATPase6 genes among groups where 3 novel missense mutations in MT - ATAase8 gene at positions (8378, 8483 and 8558), the changes of nucleotides bases were A>G, A>C, A>C and A>C respectively, replacing Asparagine to Aspartic acid, Leucine to Proline and Proline to Alanine respectively. Also two novel missense mutations were observed in MT - ATPase6 gene at positions nt (8822 and 9055) where the changes nucleotides were (C >T and G>A) that replaced amino acids (Serine to Phenylalanine and Alanine to Threonine) respectively. Silent novel heteroplasmic change as a transition substitutions in ATPase8 gene at position nt (8371) by replacing C>T without any changing in amino acid of protein. Results of this present study showed novel heteroplasmic silent mutations in ATPase6 gene at positions nt (8899, 9048 and 9060) nucleotides changes (C>T, T>C and C>A) respectively, without changing in amino acid of protein which were observed in infertile group

تشخيص بكتريا Neisseria gonorrhoeae بالطرق التقليدية والجزيئية في المرضى الذكور ودراسة مدى تاثيرها في حدوث الحذوف في موقع AZF == Conventional And Molecular Diagnosis of Neisseria Gonorrhoeae In Male Patients And Study Its Suspected Effects In Microdeletions In Azf Locus

اسم المؤلف: غزوان علي مسلم الرماحي
اسم المشرف: عبد الرضا عبد الحسن اللامي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: This study includes 82 clinical samples from male patients suspected to have gonorrhea (urethral discharge with dysuria). Two types of samples were collected from each patient, urethra swab and blood samples from patients who attended Al - Yarmouk teaching hospital in Baghdad city, Clinics and private laboratories during a period from December 2012 to April 2013. All of them were married and have children's, and 20 samples were taken from apparently healthy subjects.Cases caused by gonococci were diagnosed by finding the Neisseria gonorrhoeae bacteria in the samples using microscopic examination and culturing on rich media chocolate agare for growth bacteria and selective media modified Thayer martin agar (MTM) contact antibiotic to prevent growth of all the types of bacteria except N. gonorrhoeae.Microscopic examination using specific kit for gram stain, showed gram negative diplococcus, like bean shape. Culturing on rich media revealed that 82 (100%) samples positive but re culturing on Modified Thayer martin media revealed that 76 (92.68%) out of 82 positive. Then biochemical test had the same results.Results of the molecular diagnosis of the bacteria causing gonorrhea using specific primers that were specific for the Orf1 gene, revealed that 80.26% of samples (61 out of 76) were positive. Results of the microdeletion in Y chromosome AZFc region revealed that no microdeletion were occurred in SY - 254 STS and BPY - 2 gene.This study, provided high specificity and sensitivity for the diagnosis of gonorrhea using PCR technique which is cheaper and faster than traditional methods. Also, PCR - based method for detection of N.gonorrhoeae can be readily used in hospitals and laboratories.

دراسة بعض المؤشرات المناعية لدى بعض المرضى المصابين بفيروس المليساء المعدية في محافظة ديالى == A Study of Some Immunological Parameters In Some Patients With Molluscum Contagiosum In Diyala Province

اسم المؤلف: رغد ياسين اعويد
اسم المشرف: عباس عبود فرحان الدليمي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: المليساء المعدية مرض يسببه فيروس (MCV) الذي ينتمي لعائلة POXVIRUS.المليساء المعدية حيث انه مرض غير قاتل وشائع في جميع انحاء العالم, قد يشترك مع اورام المليساء التهابات قليله, المليساء المعدية يستمر لشهور او سنوات..اجريت الدراسة الحالية للفترة من 1 تشرين ا | The molluscum contagiosum (MC) virus (MCV) is a dermatotropic poxvirus, and the causative agent of MC. MC is nonlethal, common andworldwide. Additionally, little inflammation is associated with MCpapules, and MC can persist for months to years. The present study was conducted for the period from1 November 2013 to 30April, of 2014 in outpatient clinic of Baquba Teaching Hospital in city. The study aimsat assessing the immune status of patients infected with molluscumcontagiosum through the measurement ofthelevel of immunoglobulin(IgG, IgM)and the level of complementcomponents especially (C3, C4) by radial immune diffusion assay and measurement of the level of interleukin 18 R1 by Elisa assay which immune responses are key for the eventual resolution of MC. 75 patients were diagnosed with clinical lesions of MCV on different areas of the body, age of patients ranged from(2 - 50 years) including 40(53.3%) males and 35(46.7%) females.The control were 26.6±15.4 years (range from 2 - 50 years), 8 (53.3%) of them were males and 7(46.7%) females.After theexamination by single radial immune diffusion and Elisa kit.The results showed the patientswere equal or less than 16 years, 24 (32%) from 17 - 30 years, 19 (25.3%) from 31 - 45 years and 12 (16%) above 45 years and 40(53.3%) males and 35(46.7%) females, no static significant difference showed between the MCV infection and either the sex or age.The results revealed the level of IL 18R1in patient the mean±SD were 677.15±874.22 ng/ml while in control were 178.46±31.79 ng/ml. There was significant statistical difference between both groups as patients with MC had high level of IL18R1 than control.Themean±SD of IgMinpatients were 1946.6±825.6 mg/dlwhile in control were 140.1±68.7mg/dl This result was highly significant which indicates that patients with MC had higher level of IgM than control In contrast, patients had lower levels of IgG than control, The mean±SD of IgG in patientwere 221.9±96.7mg/dl while in control were 1229.9±299.7mg/dl. This result was highly significant. Also the patients had lower level of C3 and C4 than control. The mean±SD of C3 in patients were 109.6±64.8mg/dl while in control were 120.8±22.1mg/dl. The mean±SD of C4 in patients were 27.8±12.7mg/dl while in control were 38.7±9.8mg/dl. These results were statistically significant. Eleven 9 (14.7%) of patients had positive CRP while 12 (80%) of control had positive CRP which was statistically highly significant. Forty seven (62.7%) patients were from rural area while 28 (37.7%) from urban. There is no statistical difference between both groups as. Family history of MC and Recurrence of MC in patients were nostatically significant difference to infection by MCV.

دراسة امكانية الاستفادة من تركيز بروتين المصل الفعال نوع سي بروتين التفاعلي CRP في التشخيص المبكر للاصابات البكتيرية لحديثي الولادة وتحديد توقف اعطاء العلاج من خلاله == The Study of Usefulness of C - Reactive Protein Concentration In The Early Diagnosis And Determining The Duration of Antibiotic Therapy of Suspected Neonatal Bacterial Infection

اسم المؤلف: قاسم ابراهيم حسين
اسم المشرف: ايمان ناطق ناجي البياتي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: ان التشخيص السريع لاصابات تجرثم الدم في الاطفال حديثي الولادة من الامور المهمة وذلك لخطورة الاصابة المؤدية الى الموت.الغرض من دراستنا هو امكانية استخدام فحص سي - البروتين الفعال في التشخيص المبكرلهذا المرض البكتيري وكذلك استخدامه في تحديد نقطة التوقف عن ا | Rapid diagnosis of bacterial infections of newborn infants is very important itcause of morbidity and mortality. Aim of the studyThe aim of the studyis to examin the possibility of using C - reactive protein in the early diagnosis of neonatal sepsis, as well as its use in determining thestop point of the antibiotic treatment.Patent, Materials and methods This study was conducted at the Hospital of Ibn - Albaladi for children and women in Rusafathe Healthoffice / Baghdad.Iraq from February 2013 to February 2014.The number of patients with bacterial infection was 102 (62%) of the total number of patients (165) who were admitted to the neonatal care unit aged less than 28 days and their weights was more than 1500 g,. The number of males was 92 (56%) and the number of females was 73 (44%), the number of patient withearly onset sepsis EOS (less than three days) was 43 (42%) and with late onset sepsis LOS for (more than three days) was 59 (58%), 43 (57%) ofEOSwas normal deliveries infected and cesarean deliveries infected 33 (43%), while51 (57%) of LOS was normal deliveries infected and cesarean deliveries in LOS was 38 (43%). Blood samples were collected and divided into three portion : bloodculture, CRP andhematological profile.1 - Blood culture.Blood was collection from neonatal sepsis patientinto special blood culture bottle and incubate in bact /Alert 3 D device when growth of bacteria then subcultured on blood agar, MaCconkey agar, and incubatedusingvitek /2 to diagnose bacterial infection anddeterminetheir susceptibility to antibiotic treatment.ResultThe Compact results indicated that 63 (38%)of patient were negative and 102 (62%) were positive out of them 23 patients (22.6%)were infected with gram negative bacteria including {Klebsiella pneumonia 30%, Acinetobacterlowffi 17%, Enterobacter cloaca 13%, } while 79 (77.4%) were infected with gram positive bacteria including { staph Homins and staph hemolyticus each 28% then staph epidermidis 13% and staph aureus 10%, while strep spp was 6% }. 2 - c - reactive protein.It was done by twomethod for the same samples in order to characterize the accuracy of the diagnosis. The first method was agglutination test (method 1) the cut off value for positive result ? 6 µg % 79 (48%)were negative, while 86 (52%) werepositive, while the second method was immune test (method 2) µµµusing Nycocard device werethe cut off value for positive result ? 5 µg % the results 102 (62%) were positive while 63 (38% negative.3 - hematological profile.The hematological profile of our patient included PCV, WBC, neutrophil, lymphocyte, monocyte, count were measured using Emerald automated Hematology device. The results of these tests werenot compatible with the results of the blood culture, but normal result were compatible with negative blood cultures. The results adopted of blood culture confirm the presence of bacterial infection and then compared with theresults CRP test and blood profilefor the same samples.Negative result of blood culture and CRP test were 54 (33%) while positive results of blood culture and CRP test were recorded and improved after taking treatment for (, 3, 5, 7,) days for (40, 31, 5)patients respectively.ConclusionWeconcluded C - reactive protein test was useful in the diagnosis of bacterial blood infections and determine the stop - point otreatment. Gram negative bacterial isolates were sensitive impinim, livofloxacin and Amikacin while they were resistant to Ampicillin, Ampicillin /Sollbectam and Gentamycin. on the other hand gram positive bacteria were sensitive to Amikacin, Ampicillin/sulbactam Ciprphloxacin, and Tigecyclin and were resistant to Erythromicin, and Oxacillinand Benzylpencillin.

دراسة كفاءة بعض المضادات الحيوية ذات المناشئ المختلفة على بعض انواع البكتريا المعزولة من حالات مرضية مختلفة == The Efficacy Study of Some Antibiotics From Different Origins On Some Pathogenic Bacteria Isolated From Different Cases

اسم المؤلف: ليلى عاصي خزعل
اسم المشرف: نجدت بهجت مهدي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: كركوك
الصفحات الاولى:
المستخلص: شملت الدراسة جمع 278عينة سريرية تضمنت نماذج من اخماج المجاري البولية ومسحات الجروح والحروق وعينات الدم ومسحات الاذن الوسطى من مستشفى كركوك العام ومستشفى طوز العام وللمدة من ( 2013 - 4 - 15) ولغاية ( (2013 - 10 - 15. اظهرت نتائج الزرع البكتريولوجي الاولي | The study included the collection (278) clinical samples included the same of urinary tract infections and swabs of wounds and burns, and blood samples and swabs from ear from Kirkuk General Hospital and Tuz General hospital in period from (2013 - 4 - 15) until (2013 - 10 - 15). The results of the first Bacteriological implant using the blood Agar and Macconkey agar emergence of planting positive in (148) of the total sample (278) sample rate (53.33%). Diagnosed colonies developing depending on planting characters and biomicroscopy and tests biochemical as possible diagnosis (90) the isolation of clinical divided to (30) isolation of each of the bacteria (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa) divided to (28) isolates of Urine and 21 isolated from wounds and 25 isolates of burns and 11 isolated from blood and 5 isolates from the ear of the total isolates, confirmed diagnosis using the API Staph System and API 20E system. The study included examination of sensitivity using discs toward the 15 antibiotic for three types. The isolates showed to different in resistant rate, and was Anti Chloramphenicol is the most effect on the bacteria S.aureus where all isolates were sensitive to him 100%, and was an anti Imipinem, Nitrofuranation the most effect on bacteria E.coli where the percentage of sensitivity to two anti - (90%), either Anti Amikacin was the most impact on the isolates of Ps. aeruginosa where all isolates were sensitive to him ratio (100%) I tested the sensitivity of bacterial species toward (5) antibiotic (Amoxicillin Augmantine, Cefotaxime, Ciprofloxacin, Tetracyclin) concentrations of certain several different origins for each antibiotic using the method of casting dishes, use an anti Amoxicillin concentration (25) mg / ml and origins different (Iraqi, Indian, Turkey, United Arab Emirates) The results show that the origin United Arab Emirates is the most efficient than the rest of other. Use the adversaries (Cefotaxime, Augmantine) concentration (10) mg / ml and three - origins (Turkish, Indian, United Arab Emirates), oukd show that Origin United Arab Emirates is the most efficient of the rest of the origins Other. also used anti Tetracyclin concentration (10) mg / ml for three origin (Iraqi, Indian, Chinese). show that Origin Iraqi is the most efficient of the rest of the origins Other.and used anti Ciprofloxacin concentration (5) mg / ml for four origin (Iraqi, Indian, Turkish, United Arab Emirates) and show that the United Arab Emirates and Turkish origin is more efficient than the rest of other origins.Bacterial isolates showed multiple drug resistance to antibiotics where all isolates Ps. aeruginosa with resistance to multiple antibiotics by (100%) and showed isolates of S.aureus resistant multi - rate (93.3 %) and showed isolates of E.coli (96.6%) as well characterized bacterial isolates in multiple resistance to antibiotics Alpittalaktam particularly where the rates of multiple resistance (93.3 %) of the isolates of E.coli and (100%) isolates Ps.aeruginosa and (93.33%) of the isolates by S.aureus.the rapid Iodine standard method was used for screening for susceptibility of bacterial isolates to produce enzymes Beta - Lactamase have shown (73) the isolation of a positive result from the total (90) isolation rate (81.1%), were distributed among the isolates produced (27) isolated from bacteria by S.aureus (90 %) and (25) isolates of E.coli bacteria by (83.3%) and (21) isolated from bacteria Ps. aeruginosa (70%).to detect broad - spectrum Beta - Lactamase enzymes is used discs method adjacent and the results showed that there are (24) isolation of the total (73) is positive for examination by (32.87%) distributed on (12) isolated from bacteria S.aureus by (44.44%) and (5) isolates of E.coli bacteria (20%) and (7) of the bacterial isolates Ps. aeruginosa by (33.33%).Tested susceptibility bacterial isolates to produce mineral Beta - Lactamase enzymes Metallo ? - Lactamase using IMP - EDTA Combination disc and three isolates can from the production of the enzyme (4.10%) by two isolates of bacteria Ps.aeruginosa by (9.52%) and one isolate of the bacterium E.coli (4%).

تحديد شدة الاصابة الحادة والمزمنة لداء المقوسات الكوندية بواسطة مستويات (IL - 6, IL - 8, TNF - ?) == Determination of Acute And Chronic Toxoplasmosis By Estimation of IL - 6, IL - 8 And TNF - ? Levels

اسم المؤلف: لينا قاسم كاظم الزهيري
اسم المشرف: بان نوري القاضي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: المقوسات الكونديه من الطفيليات الشديده النجاح في انشاء اصابة مزمنه طويلة الامد. وقد اجريت الدراسة الحاليه على 366 امراة (117 مجهضات، 141 حوامل، 108 غير متزوجات) جمعت من مستشفيات مختلفة في بغداد. جمعت عينات الدم لجميع المجاميع المدروسة خلال الفترة مابين تش | Toxoplasma is a highly invasive parasite which establishes a life - long chronic infection. The present study was performed on 366 women (117 miscarriages, 141 pregnant and 108 singles) aged (17 - 35) years old from different hospital in Baghdad. Blood samples were collected from all patients during the period of Oct.2013 until of Jan. 2014. Anti - toxoplasma antibodies in the sera of all samples by using two tests, Latex test (LAT) and ELISA (IgG), (IgM) while, the proinflammatory cytokine were estimated by evaluation of the cytokine such as (IL - 6, IL - 8, TNF - ?). The results were showed that : ? The infection rate of toxoplasmosis by LAT was (41.53%) which was more sensitive than ELISA IgG (36.85%), and miscarried women were diagnosed by higher anti - toxoplasma IgG antibodies 62/117(52.99%), while pregnant result were lower 30/108 (27.78%). ? The most frequent age group of chronic infection with T.gondii was (23 - 28) year in both miscarriage and pregnant women, and represented by 45.16%, 40% respectively. While the most frequent age group for a single woman was (17 - 22) years, and represent 46.511% of the total number of this group.? Seroprevalence of toxoplasmosis by ELISA IgM test revealed low sensitivity in different studied groups 25/366(6.830%), and miscarried women were diagnosed by a highly significant (P ?0.01) percentage of acute toxoplasmosis14/117(11.965%).and The age group of (17 - 22) years old diagnosed high significant (p?0.01) percentage of acute toxoplasmosis in all studied groups.? According to the distribution of infected miscarried women with toxoplasmosis and gestational age by ELISA IgM the result showed that most of miscarriages were occurred at the first trimester which considered high significant (p? 0.01) 8/14(57.42%). While, chronic infected women at the second trimester (4 - 7 month) diagnosed by significantly (P?0.01) higher percentage of miscarried 24/62(38.71%).? The level of pro - infalmmatory cytokine (IL - 6) in acute infected miscarried women with toxoplasmosis distincted with a significantly high increase (P? 0.05) (177.31±12.53 Pg/ml) in comparison to chronic infected one (119.36 ±14.07 Pg/ml).While, chronic infected pregnant women was low significantly (P ?0.01) (48.41± 2.79 Pg/ml) in comparison to acute infected one and other studied groups, ? The acute infected singles was (118.98± 15.68 Pg/ml) higher non - significantly (P?0.05) than chronic infected one (112.65 ± 17.91 Pg/ml) and both disease activity were significantly higher than its level in healthy singles.? The level of TNF - ? in acute infected miscarried women distincted with significantly higher (P?0.05) (97.46 ± 5.37 Pg/ml) in comparison to chronic infected women (69.55 ± 4.58 Pg/ml), Whereas the mean level of TNF - ? in pregnant infected women during acute phase (62.31 ± 3.82 Pg/ml) was significantly (P?0.05) higher than chronic infection (21.42 ±2.74 Pg/ml)? Single infected women showed high significant increase (P?0.05) of TNF - ? level (50.48 ± 7.42 Pg/ml) in comparison to chronic infected one (35.46± 5.36 Pg/ml) and both disease activity scored high significant increase (P?0.05) of TNF - ? in comparison to healthy singles (18.29 ± 1.02 Pg/ml).? Finally, the mean level of IL - 8 in miscarried women with positive infection with T. gondii during acute phase (187.92 ± 15.38 Pg/ml) was significantly (P?0.05) higher than chronic phase of infection (140.57 ± 13.87 Pg/ml).Whereas the mean level of IL - 8 in acute infected pregnant women (110.32 ± 9.13 Pg/ml) was higher significantly (P?0.05) than healthy pregnant (91.49 ± 7.59 Pg/ml) and healthy singles (88.82 ± 5.62 Pg/ml).? Infected single women showed highly significant (P?0.01) increased levels in both diseases activity in comparison to single women without infection (88.82± 5.62 Pg/ml).

دراسة بكتريولوجية لبعض الاجناس البكتيرية السالبة لصبغة غرام المقاومة لمضادات البيتالاكتام والمعزولة من اخماج المجاري البولية في محافظة ديالى == Bacteriological Study of Some Genus For Bacterial Gram - Negative Resistant To ? - Lactam, Isolated From Urinary Tract Infections In Diyala Province

اسم المؤلف: محمد خضير عباس النعيمي
اسم المشرف: عباس عبود فرحان الدليمي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: شملت الدراسة 300عينة جمعت من مرضى مصابين باخماج المجاري البولية وتم جمع العينات في مدينة بعقوبة من مستشفى البتول للولادة والاطفال ومستشفى بعقوبة التعليمي، للمدة بين 1/9/2013ولغاية1/1/2014.اظهرت نتائج الزرع البكتيري على اوساط اكار الماكونكي واكار الدم ووس | Three hundred urine from patients suffering from urinary tract infection from Baaquba Teaching Hospital and Al - Batool Hospital in Baaquba city for the period from 1/09/2013 to 1/01/2014. The results reved that 66 isolates are belonging to bacteria of Gram negative (57.4%), 25 (37.78 %) Escherichia coli, 22(33.33%) Proteus mirabilis, 9(6.06%)Klebsiella pneumonia, 5(7.57%) Enterobacter spp for 4 (25%) Enterobacter cloacae and 1(5.51%) Enterobacter aerogenes and5(7.57%) Pseudomonas aeruginosa by using diagnostic phenotypic, biochemical tests and confirm the diagnosis using regular aPI20E. Investigation of some virulence factors showed that both Escherichia coli and Proteus mirabilis produced haemolysin 52% and 90.9% respectively. The production of biofilm by local isolates was detected in Congo - red way, Isolates of Escherichia coli and Proteus mirabilis had been shown the ability to produce biofilm 92% and 90.9% respectively. The results showed that all isolates of Proteus mirabilis produced urease by 100%, while the results detect that the isolates of Escherichia coli not able to produce this enzyme. Proteus mirabilis showed ability to produce swarming with 100%. Siderophore production by Escherichia coli and Proteus mirabilis was 48%, 9% respectively. Eschrichia coli, and Proteus mirabilis produced bacteriocin with percentag of 32% and 50% respectively.The production of ? - lactamase by Eschrichia coli and Proteus mirabilis was 60% , 40.9% respectively, also the isolated had the ability to produce the Extendended spectrum ? - Lactamase enzyme by using disc Approximation, The production from each ofEschrichia coli and Proteus mirabilis 12%, 31.8 % respectively. Results of Metallo? - Lactamase by using the Imp - EDTA combination indicated that E.coli and P.mirabilis were 12% and 13.6% respectively. Isolates of E.coli showed highest resistance rate 92% for Augmentin while isolates of P.mirabilis showe higher resistant to cefotaxime 81.8%. The results showed that multiple resistance pattern for antibiotic 43 (91.5%) resistant to (2 - 5) antibiotics, Isolates of E.coli showed highest multiple resistance rate 92% while isolates of P.mirabilis rate 90.9%. The Minimal inhibitory concentration (MIC) for namely two antibiotics Cefotaxime, Ceftazidime were determined.MIC values of these antibiotics ranged between (2 - <1024), (4 - <1024) µg\ml E.coli and (2 - <1024), (8 - <1024) µg\ml P.mirabilis respectively. The results of molecular detection of ESBL genes (bla TEM and bla SHV) by using PCR technique, (9) samples from (10) total, divided into 3(100%) E.coli and 6(85.7%) P.mirabilis were harboring bla TEM gene based on the presence of 950 bp bands in 1% agarose gel. while results detect that the isolates of Escherichia coli and Proteus mirabilis not harboring bla SHV gene.

دراسة وراثية وبكتريولوجية على بكتريا تثبيت النيتروجين التكافلية Ensifer meliloti == Bacteriological And Genetic Study On Symbiotic Nitrogen Fixing Bacteria Ensifer Meliloti

اسم المؤلف: محمد زهير ناجي المرعب
اسم المشرف: حسن فاضل ناجي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: عزلت عشر عزلات من بكتريا Ensifer meliloti من العقد الجذرية لنبات الجت Medicago sativa L. بعد ان جمعت من مناطق مختلفة من محافظة بابل خلال المدة من شهر تشرين الثاني/2012 لغاية شهر شباط/2013. شخصت العزلات جزيئيا بتقنية تفاعل البلمرة السلسلي فضلا عن طرق ال | Ten isolates of Ensifer meliloti were isolated from root nodules of Medicago sativa L. collected from different areas of Babylon province during a period from November - 2012 to February - 2013. The isolates were molecular identified using polymerase chain reaction technique, in addition to traditional methods (morphological and biochemical). All isolates were harboured nodbox and mucR genes and produced cyclic ? - (1, 2) glucans, cellulose fibers and lipopolysaccharide.The ability of isolates to grow at different degrees of pH, temperatures and different concentrations of sodium chloride were tested. It was found that the optimum pH for growing of Em1, Em3, Em4, Em6 and Em8 isolates was pH7, while for the isolates Em2, Em5, Em7, Em9 and Em10 was pH8. The optimum temperature for growing of Em1, Em3, Em5, Em7, Em8 and Em9 isolates was 30?C, while the optimal growth of Em2, Em 4 and Em6 isolates was 35?C, Em10 isolate showed significantly extremism to grow at temperture reached to 45?C. The optimal percentages of NaCl for growing of Em1, Em3, Em4, Em5, Em6, Em8 and Em9 isolates were 2%, for Em7 isolate was 3% and for Em2 and Em10 isolates were 4%. The effect of different degrees of pH on symbiotic properties was studied. Em1 and Em8 isolates that grew at pH6.5 ; all isolates that grew at pH 7, pH 7.5 and pH 8 were able to infect the root hairs of alfalfa plants. The isolates that grew at pH6.5 (except Em1 and Em8 isolates) and all isolates that grew at pH8.5 were failed to infect the root hairs and did not formed nodules and resembled to the control plants (without inoculum).Different concentrations of NaCl on symbiosis was studied, it was found that all isolates that grew at 2% and Em2 and Em10 isolates that grew at 3% formed nodule, while Em1, Em3, Em4, Em5, Em6, Em7, Em8 and Em9 isolates that grew at 3% and all isolates that grew at 4%, 5% and 6% did not elicited nodules on the roots of alfalfa plant. Light microscopic observations of root hairs inoculated with Ensifer meliloti isolates showed the root hair deformations like curling, waviness, swelling and formation of shepherd`s crook and infection thread. These consequent changes lead to emerge the mature nodules having five distinct zones viz., distal meristematic, infection, inter, nitrogen - fixing and proximal senescence zones.The gel electrophoresis results of plasmid DNA of isolates showed that these isolates harboured two megaplasmids. When the isolates exposed to extreme factors like pH, temperture and NaCl, it was found that the isolates lost these plasmids and its ability to elicite the nodules and failed to fix the nitrogen. This study showed that the nitrogen fixing bacteria had essential role to support the plant growth and increase their productivity, and recommended to use the isolate Em10 as example for biofertilizers and the best alternative to the chemical fertilizers

دراسة التاثيرات السمية للـ Gliotoxin المنتج من العفن Aspergillus fumigatus باستخدام تخمرات الحالة الصلبة == Study Toxicity Effects of Gliotoxin Produced By Aspergillus Fumigatus Using Solid State Fermentation

اسم المؤلف: محمد عادل نوري
اسم المشرف: عبد الكريم جاسم هاشم
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم اختبار قابلية عشرة عزلات للعفن Aspergillus fumigatus على انتاج الغليوتوكسين بوساطة تخمرات الحالة الصلبة وباستخدام الرز كوسط زرعي (ركيزة). اشارت نتائج الغربلة ان العزلة AF - 5 كانت هي الافضل واعطت اعلى انتاجية.كانت الظروف المثلى لانتاج السم على وسط الر | The ability of Ten Aspergillus fumigatus isolates for gliotoxin production were screened by solid state fermentation (SSF) using rice medium as substrate. The results indicated that, the AF - 5 isolate was the highest gliotoxin producer.The optimum conditions for gliotoxin production by AF - 5 isolate on rice medium were : - inoculum size 6×106 spores, moisturizing ratio (5 : 1) (w : v) with distilled water and incubation at 37 ?C for 10 days. Gliotoxin was purified from crude extract of AF - 5 isolate after production under the optimum conditions using solid state fermentation SSF. Purification of gliotoxin was achieved by two steps including : filtration and solid phase extraction. These processes were accessed to remove debris from crude extract and gives pure gliotoxin. The gliotoxin concentration was (122.6 ppm), which was detected by Thin layer Chromatography and High Performance Liquid Chromatography.The result of cytotoxicity effect of both purified extract and standard gliotoxin on human lymphocytes with exposure time of 24 hours at four different concentrations 25, 50, 100 and 200 ppb showed growth inhibition percentage 21, 39.10, 61.99, 87.45% and 17.89, 34.92, 58.34, and 85.22% respectively. The cytotoxicity effects have been done using conversion of the MTT to MTT formazan. After DNA was extracted from lymphocyte treated with toxin and analyzed by electrophoresis on a 1% agarose gel, gliotoxin appeared to have ability to damage the DNA. Results showed that both growth inhibition and DNA damage were increased gradually with the increasing of gliotoxin concentration.

دراسة انتشار انواع الفطر Saprolegnia spp. في مياه مدينة الديوانية وتوصيفها جزيئيا == Studying The Spreading of Fungus Saprolegnia SPP. In Waters of Diwaniyah City And Characterization The Molecularly

اسم المؤلف: معتز محمد عزوز الزاملي
اسم المشرف: ماجد كاظم عبود الشبلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: شملت الدراسة الحالية عزل وتشخيص بعض الفطريات المائية ومنها انواع الفطر Saprolegnia spp. في مياه مدينة الديوانية وتوصيفها جزيئيا. عزلت الانواع التابعة لهذا الفطر وهما Saprolegnia parasitica وSaprolegnia ferax من الانهار والبحيرات والاسماك, وتم قياس الخصائ | The present study is concerned with isolation and identification of some aquatic fungi, including species of Saprolegnia spp. in waters of Al - Diwaniyah city and molecular characterization. Two species, Saprolegnia parasitica and Saprolegnia ferax, have been isolated from rivers, lakes and fishes. The physical and chemical environmental characteristics have been measured. The types above have been examined to extract the DNA. The (PCR) technique has been used to examine the presence of protease enzyme. The results of isolation and identification showed that the percentage of the two species were 64% in rivers areas 40% in the lakes areas and 50% in fishes. The rates represented the examined samples which were 60 samples. AS well as isolates was in rivers 16 isolation and in Lakes 10 isolates either in fish was 5 isolation and thus the total isolates 31 isolation, The S. parasitica species is more apparently found during (February, 2014), the number of samples was 12, the less presence of this species in (April, 2014), where the number of sample, was 3. The S. ferax species is more apparently presented in (February, 2014), the number of samples was 10. The less presence was in (April, 2014), the number of samples was 2. The results showed that the number of isolation of Saprolegnia spp. study area as it was S.parasitica species in were 6 in rivers, 2 in lakes, and 3 in fishes. The second species, S. ferax showed number of isolations which were 10 in rivers, 8 in lakes, and 2 in fishes. The PCR examination has used to detect the rDNA gene which responsible for proteases gene and whether it is found in the two species of fungi. The express way has been used to extract DNA and according to the instructions of manufacturer company. The concentrations of DNA in first species were 121.8 - 73.0 mcg/microliter and purity 1.87 - 1.80.In the second species the concentrations of DNA were 2360.4 - 166.9 mcg/microliter and purity 2 - 1.75. The results, by using PCR technique, showed that the percentage of protease in first species was 25% in rivers. In lakes and fish was 100%.Concerning the second species, the percentage of enzyme was 42% in rivers, 40%in lakes, and 50% in fishes. It can be concluded that there is a diversity in fungi species with respect to study areas and enzyme secretion in the stages of fungi infection. The enzyme is more apparently found in S.parasitica species compared with S.ferax species.

دراسة مصلية دمية لداء المقوسات في النساء الحوامل والاطفال حديثي الولادة في محافظة القادسية == Bloody Serological Study of Toxoplasmosis In Pregnant Women And Newborns In Diwaniyah Province

اسم المؤلف: مروة جبار مزهر العبادي
اسم المشرف: خيري عبد الله داود العكيلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية على الاطفال حديثي الولادة في محافظة الديوانية للمدة من بداية شهر تشرين الثاني 2012 الى نهاية شهر نيسان 2013.وقد جمعت عينات الدراسة من دم الحبل السري للاطفال الرضع المولودين حديثا اثناء الولادة في مستشفى الاطفال والولادة التعليمي في | The present study was done on new born babies in Diwania province during a period from the beginning of November 2012 till the end of April 2013. Blood samples were collected from the umbilical cord of the new born babies during delivery in children and maternity teaching hospital. Fifty samples were collected separated to serological tests and for blood pictures. Serological tests were used in this research as follows (Rapid IgG - IgM test, latex test and ELISA IgG, IgM). The results showed the Rapid test was the easiest and cheapest test for diagnosis specific antibodies against Toxoplasmosis, therefore the positive samples were 14% for IgG, 4% for IgM and 36% for overlap cases. While the positive results for latex test was 14% but the highest result was recorded by using ELISA test 30% for IgG, No positive result recorded for IgM. There were variation in positive cases of different ages of mothers, the highest percents found in ages between 30 - 39 years old by using Rapid test 72.72% , while by using The latex test demonstrate high percent of infection was 20% and also for ages between 15 - 19 year old, and the ELISA recorded 50% and the same group of age 15 - 19 years old. The results showed the for the residence factor by using Rapid test showed the rural people were high percent of incidence of Toxoplasmosis 57.14%, but the urban people recorded Latex test and ELISA)17.24%, 31.03%) respectively. Aborted women in previous time were followed by using the serological tests Rapid test 10%, Latex 10% and ELISA zero. The incidence of Toxoplasmosis was high in housewives more than other career, the results were) 34.14%, 34.14 (% for Latex and ELISA, IgM and overlapping cases, while in other career by using Rapid test 66.66% Results showed standard Blood parameters did not register significant differences (P> 0.05) for the total number of white blood cells WBC, hemoglobin Hb, blood volume compact PCV, Erythrocytic RBC, and differential count of white blood cells for Neutrophiles, Lymphocyte, Monocytes, Basophiles and Eosinophiles. Phagocytic activity was prominent in adult people more than newly born babies.

تحديد بعض المعادن الثقيلة والتلوث البكتيري في الحليب الخام والمستورد المجفف == Determination of Some Heavy Metals And Bacterial Contamination of Raw And Imported Powder Milk

اسم المؤلف: مروة صباح طالب
اسم المشرف: محمد نافع علي العزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يتعرض الحليب للعديد من الملوثات الفيزيائية والكيميائية والبيولوجية وذلك بسبب طرق الانتاج واسلوب النقل وعمليات التسويق ومن هذه الملوثات المعادن الثقيلة والرقم الهيدروجيني ودرجة الحرارة والبكتيريا. وقد صممت الدراسة الحالية لفحص عينات للحليب الخام التي جمعت | Milk is subjected to various physical, chemical and biological pollutants duo to producing, transporting and marketing processes such as certain heavy metals, pH, temperature and bacteria. The current study was designed to examine fresh milk samples were collected randomelly at early morning from six different locations at a rate of once each month during study period which commenced in October 2013 and ended in march 2014 and powder milk brands which were examined again for six months as in case of fresh milk where these milk brands were collected from local markets each month. The results of heavy metals showed that : • The investigation of Lead content in raw milk revealed that the highest mean value (1.801±0.311 ppm) was scored in Abo - Ghraib sample and the lowest mean value (0.941±0.104 ppm) was observed in Ghazaliya sample. For the Cadmium content in raw milk that the highest mean value (1.532±0.124 ppm) was found in Abo - Ghraib sample and the lowest mean value (0.063±0.044 ppm) was found in Fal'loga. The Copper content in raw milk also investigated and resulted the highest mean value was (0.931±0.092 ppm) in Azizia milk sample and almost similar value (0.931±0.301 ppm) was found in Madain sample while the lowest mean value was (0.308±0.029 ppm) in Fal'loiga sample. The highest mean value of Potassium in raw milk was recorded in two samples in Azizia (9718.8±246.6 ppm) and (9718.3±482.2 ppm) in Abo - Ghraib but the lowest mean value (4156.3±268.3 ppm) was recorded in Essaouira. Regarding Sodium content in raw milk, the results have found that the highest mean value was (2968.8±312.2 ppm) in Essaouira sample while the lowest mean value was (1432.5±156.8 ppm) in Abo - Ghraib. The Chloride content in raw milk samples, the highest mean value was (3053.0±128.6 ppm) recorded in Fal'loga sample and the lowest mean value was (639.0±198.4 ppm) was found in Azizia sample. Analysis of variance of these data showed significant effects (P?0.001) of both sampling sites and collecting months on milk (pb, Cd, K, Na and Cl) contents while analysis of variance of these data reveals no significant impact of sites on milk Cu content (P> 0.05) where no differences were observed between mean value of different collecting sites but shows highly significant (P? 0.001) effects of collecting months.• While powder milk has shown that the highest mean values of Lead (1.225±0.052 ppm) were found in Al - Mudhish brand and the lowest mean value (0.088±0.002 ppm) was detected in Anchor. The highest mean Cd value was (0.184±0.005 ppm) in Nido brand and the lowest mean value was (0.069±0.003 ppm) in Altunsa sample. While the highest mean value of Copper was (1.656±0.254 ppm) in Dielac powder brand and Anchor milk brand had the lowest copper mean value which was (0.767±0.014 ppm). The concentration of Potassium was (9625.0±462.9 ppm) which is the highest recorded in Dielac milk sample and the lowest mean value (3356.8±225.6 ppm) was found in Al - Marai milk sample. While the highest average of Sodium was (2625.0±342.6 ppm) scord in Dielac brand and the lowest average was (1745.0±338.4 ppm) recorded in Al - Marai sample. The highest mean value of Chloride (193.0±41.0 ppm) was recorded in Nido milk brand and the lowest mean value (122.7±22.2 ppm) was found in Altunsa milk sample. However, analysis of variance shows significant differences (P?0.001) between examined milk brands while no such differences were found between these brands (P>0.05) regarding collecting months.From the above results, it seems that the highest content of (Pb, Cd and Cu) are higher than those of standards of the International Dairy Federation (IDF) while contents of (K, Na and Cl) are within the standard requirement according to National Research Council (NRC).Microbial tests of raw milk samples were positive for bacterial growth, as different bacterial species were observed such as Lactobacilli spp. 63.8%, Streptococcus spp. 61.1%, Staphylococcus aureus 44.4%, Escheritia coli 88.8%, Bacillus spp. 72.2%, Salmonella typhi 72.2%, Staphylococcus epidermidis 30.5%, Pseudomonas spp. 47.2%, Micrococcus spp 27.7%, Clostiridia spp. 66.6%, Enterococci spp. 11.1% and K. pneumonia 75% from bacterial contamination of raw milk samples.However, only (11.1%) of the total collected samples of raw milk were suitable for human consumption and (88.9%) unsuitable, indicating that the source of infection or contamination of milk may be due to the animal health, human handler and the environmental factors, e.g., contaminated vessels, polluted water, flies and dust, etc… Obviously, bacterial contamination test of powder milk samples was carried out and negative results were obtained. However, it seems clearly that the opportunity of finding bacterial contamination in powder milk samples may be very rare since milk powder is produced in well designed and modern techniques. Nevertheless, a single bacterial contamination test has indicated to a positive growth of Staphylococcus aureus colonies.

تاثير المجال الكهرومغناطيسي في بعض صفات النمو والحاصل والانقسام الخلوي لنبات العصفر Carthamus tinctorius L == The Effect of The Electromagnetic Field On Some Growth Characteristics And Yield And Mitotic Index For Carthamus Tinctorius L

اسم المؤلف: مروة قيس ابراهيم العبيدي
اسم المشرف: ماهر زكي فيصل الشمري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اولا : - التجربة الحقلية : اجريت التجربة في الحقل التابع لقسم علوم الحياة - كلية التربية للعلوم الصرفة - ابن الهيثم، وصممت باستخدام تصميم القطاعات الكاملة المعشاة Randomized Complete Blocks Design (RCBD) حيث تم تعريض البذور قبل الزراعة لمجال كهرومغناطيسي | First : - Field experience : the experiment was conducted in the Department field of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using design Full sectors Randomized Complete Blocks Design (RCBD) where the seeds exposing to electromagnetic field in the Department of Physics - Electrical Laboratory - College of Education, Pure Science - Ibn al - Haitham before planting them in five intensities (0, 5, 10, 15, 20) mT and two (1.2 and 3) an hour for each intensity to see the electromagnetic field effect in some of the qualities of growth and yield plant Safflower the results were as follows : The effect of electromagnetic field strength was positive traits and was the highest increase in exposure to the intensity of 10 mT for each of the (percentage of germination, root size of the second date, plant height, leaf area, leaf area guide, the dry weight of the root system at the time the second, fresh weight of shoots at the first appointment, the average absolute growth, the sustainability of biomass, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (81.21%, 18.33 cm 3, 93.37 cm, 71.29 cm 2, 1.187, 8.21 g.plant 1, 155.13 g.plant - 1, 1.76 g.yum 1, 691.67 g, 2.03%, 0.733%, 2.47%, 12.96%, 56.96 spad, 44.55 Nora.plant - 1, 46.60 g.plant - 1, 5.35 g, 161.80 kg. acres - 1, 38.74 mg. plant - 1, 12.09%, 13.50%), respectively, except for the size of the root first date, fresh weight of the total root for the first two dates, and second, the dry weight of the root system in the first date, fresh weight of shoots in the second deadline, the dry weight of shoots for the first two dates, and second, the specific weight of the oil had the highest increase at exposing the seeds to the intensity of 15 mT and average (15.16 cm, 15.12 g. Plant - 1, 26.00 g plant - 1, 3.98 g plant - 1, 411.08 g.plant - 1, 20.03 g. Plant - 1, 69.15 g. Plant - 1, 1.069%, respectively) and the highest increase of the proportion of total oil when exposure to the severity of 5mT an average 33.94%. The effect of the length of time in field was significant in most of the qualities which gave the term two highest increase with the exception of germination percentage was the highest increase in exposure for a period of three hours, with an average (60.79%, 91.01 cm, 67.51 cm 2, 1.124, 131.93 g. Plant - 1, 337.61 g. plant - 1, 1.68 g. Day - 1, 1.92%, 12.04 g. plant, 55.99 Spad, 146.06 kg. dunams - 1, 4.77 g, 40.23 mg. plant - 1, 28.03%) for each of the (germination percentage, plant height, leaf area, leaf area guide, fresh weight of shoots in the first date, fresh weight of shoots in the second deadline, the average absolute growth, nitrogen, protein, chlorophyll, yield seeds, 100 - seed weight, carbohydrate content, the total percentage of oil). As was the overlap between field strength and duration significant effect on most of the traits with the superiority of treatment (10 mT for two hours) on the rest of the other transactions for each of the (germination percentage, the size of the root of the first two dates, and the second, plant height, number of branches, leaf area, area guide paper, fresh weight of the root system of the first two dates and II, , dry weight of the root of the first two dates, and the second, fresh weight of shoots for the first two dates, and second, the dry weight of shoots for the first two dates, and second, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, He yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (39.33%, 16.50 cm, 19.50 cm, 103.86%, 17.00, 79.83 cm, 1.330, 16.43 g.plant - 1, 29.20 g. plant - 1, 4.16 g.plant - 1, 10.48 g.plant 1, 184.06 g. plant - 1, 416.33 g. plant - 1, 22.35 g. plant - 1, 73.25 g. plant - 1, 2.10%, 0.800 %, 3.04%, 13.15%, 60.31 spad, 49.14 Nora.plant - 1, 53.37 g. plant - 1, 6.14 g 185.97 kg. acres - 1, - 1.plant 47.81 mg, 2.28%, 14.25%), respectively, with exception each of the average of the absolute growth and sustainability of biomass, where the highest increase in exposure to the intensity of 10 mT for a period of three hours and average (1.85 g.yum 1, 724.85 g.yum), respectively, and total percent of the oil was the highest increase in exposure to the intensity of 5 mT and for three hours and an average of 37.28% and the intensity of 15 mT for a period of three hours, specific gravity of oil and an average of 1.081%Second : - laboratory experiment : experiment was conducted in the laboratory of physiology of the plant Higher Studies - Department of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using the full random design Completely Randomized Design (CRD) to study the electromagnetic field effect five intensities (0, 5, 10, 15, 20) mT and two (1.2 3) per hour on the severity of the cell division of the safflower plant results were as follows : The results showed that there is a positive effect of the electromagnetic field on the traits and was the highest increase at exposing the seeds to the intensity of 10 mT for each of the (number of dividing cells of the tops of developing the parties to the roots, the total number of cells to the tops of the parties to the roots, and guide cell division developing the tops of the parties to the roots) and average (156.00% 1143.00%, 12.17%), respectively. The effect of duration of exposure, and had a significant effect on the average number of total cells to the tops of the parties to the roots reached 1042.20% when the exposure for two hours.The effect of overlap between the severity and duration of the electromagnetic field significant was effect in all cellular qualities and was the highest increase in exposure to the intensity of 10 mT for a period of one hour and average (167.00%, 13.52%) for each of the number of dividing cells developing the tops of the parties to the roots and guide cell division to the tops of developing the parties to the roots, The total number of cells to the tops of the parties to the roots was the highest increase in exposure to the intensity of 10 mT for two hours and an average of 1203.50%.

التحري عن جين الاوتولايسين في المكورات العنقوديه البشروية المقاومة لمضاد الفانكومايسين == Autolysin Gene Detection In Vancomycin Resistant Staphylococcus Epidermidis

اسم المؤلف: مريم خميس عبد ربة بريس
اسم المشرف: مي طالب فليح
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: من مجموع مئة عينة سريرية جمعت من مصادر مختلفة شملت الحروق والدم والجروح ومسحات انفية, تمكنت 90 عزلة من النمو على اكار المانيتول الملحي , ومن بينهم 40(44.4 %) عزلة عائدة لبكتريا المكورات العنقودية المنتجة للانزيم المخثر للبلازما و50 (55.5%) عائدة لبكتريا ا | Out of one hundred clinical samples were taken from different sources which include burns, blood cultures, wounds and nasal swabs infections ; 90 isolates developed growth on mannitol salt agar. Among these, 40 (44.4%) were Coagulase positive (Staphylococcus aureus) isolates, 50 (55.5%) belong to coagulase negative staphylococci in which Staphylococcus epidermidis isolates were 30(60%). The pattern of antibiotic susceptibility of Staphylococcus epidermidis isolates to 12 antibotics (Amoxiclav, Ceftazidim, ciprofloxacin, clindamycin, Erythromycin, Gentamycin, Imipenime, Penicillin G, Tetracycline, Rifampin , Methicillin, and Vancomycin) were determined using disc diffusion method. The results revealed that resistance to Penicillin G10 and Amoxiclav (Amoxicillin - clavulanic acid) were 100%, Methicillin were 93%, Erythromycin were 90%, Gentamycin and Clindamycin were 70%, Tetracycline and ceftazidim were 75%, Ciproflaxacin were 60%, Rifampin were30%. 95% of S.epidermidis isolates were sensitive to Imipenim and 5% of them were intermediate resistant, while these isolates showed 90% sensitivity to vancomycin. 19 isolates were multidrug resistance. Minimum inhibitory concentration of S.epidermidis isolates to vancomycin, was determined. The results revealed that (12) S.epidermidis isolates (40 %) were vancomycin resistant, the MIC of them were between 256 ?g\ml and 32 ?g\ml, (4) S.epidermidis isolates (13.3%) were intermediate resistance, the MIC to 3 of them were 16 ?g\ml and the last was 8 ?g\ml. Some virulence factors of VRSE and VSSE were detected including the hemolysin, protease, lipase and urease. The S. epidermidis isolates were produce hemolysin, protases, lipase and urease, were 100%, 100%, 25%, 100% respectively in VRSE, while in VSSE 100%, 100%, 60%, 100% respectively. The isolates were subjected to polymerase chain reaction (PCR) technique in monoplex pattern to amplify resistant incoding gene : the vanA, vanB and autolysine gene aae gene. The results by this study showed that 12 (40%) S. epidermidis isolates gave the implicone size (1030 base pair) of the vanA gene. However the results of MIC and PCR were similar but no any isolates gave product for presence of vanB gene. All S.epidermidis were able to produce implicone size(858bp) of aae gene. The effect of vancomycin resistant S. epidermidis on cell autolysis activity was detected by whole cell autolytic assay.The results revealed that there was significant difference among three isolates, the VSSE isolate (S.epidermidis 22) have the highest autolytic activity in the presence of antibiotic, followed by the VRSE isolate (S. epidermidis 1) and the VISE isolate (S. epidermidis14) which was the lowest autolytic activity with the presence of antibiotic. The result of transmission electron microscope (TEM) showed that the VRSE isolates (S.epidermidis 1) have thicker cell wall followed by VISE (S.epidermidis 14) isolates.However, the VSSE (S.epidermidis 22) didn't showed any cell wall thickening.

دراسة مقارنة للكشف عن الجيارديا لامبليا والطفيليات المصاحبة لها بين الاطفال في مدينة كركوك باستخدام بعض التقانات المختبرية == A Comparative Study For Detecting Giardia Lamblia And Associated Intestinal Parasites Among Children In Kirkuk City By Using Some Laboratory Methods

اسم المؤلف: مها اسماعيل مصطفى الجبوري
اسم المشرف: يحيى جرجيس سلمان
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: كركوك
الصفحات الاولى:
المستخلص: The current study had been carried out from January 2013 to July 2013 in medical laboratory researches - Kirkuk Faculty of Medicine. A total of 310 stool samples have been collected from children suffering from liquid diarrhea, their ages are from below one year to 12 years; six different laboratory diagnostic methods have been applied for detecting Giardia lamblia and other intestinal parasites. For microscopy diagnosis; direct wet double prepartions, zinc sulphate flotation are used. While immunological methods involve; Enzyme Linked Immuno - Sorbent Assay, corpo - antigen (ELISA), Direct Fluorescent Assay (DFA) and Lateral immune - chromatography assay (Triage panel). Giardia genome amplification is done using conventional Polymerase Chain Reaction (PCR) single step procedure by using of mixed primers of Giardia assemblages A1, A2 and B. The total rate of intestinal parasitic infection is 51.93 % distributed in 161 stool samples. This rate involve high frequency of protozoan infection in 132 (42.58 %) compare to 29 (9.35 %) for helminthes, P<0.05. More common intestinal protozoan parasites were Giardia lamblia 63(20.32 %) followed by Blastocyst hominis, Cryptosporidium parvum, Entamoeba coli, Entamoeba histolytica, Iodomoeba butschili, Endolimax nana and Balantidium coli with the rates : 6.77%, 6.45%, 4.18 %, 2.58 %, 1.29 %, 0.64 % and 0.32 % receptively. Concerning intestinal helminthic infection, high number and rate 23(7.41 %) was with Hymenolepis nana is compare with 1(0.32 %) record for Ancylostoma duodenale.According to gender high rate of giardiasis is recorded among males than in females, conversely to high rate of other intestinal parasites among female than in males P<0.05. Statistically relationship between Giardia distribution and ages are not significant P>0.05. Giardia co - existence are highly detected with Balstocystis hominis, and Cryptosporidium parvum total Giardia mixed parasitic infection rate 8.06 % is lower than12.23 %of pure Giardia lamblia infection, P>0.05. Regarding Giardia lamblia detecting according to laboratory methods; high rate of giardiasis 22.29% is reported by using PCR technique, followed by 20, 23 % by using direct wet preparation technique, P<0.05.The efficacy of laboratory methods for detecting Giardia stages are reduced; the following rates 19.35%, 19.03%, 17.74% and 14.51% obtained by using ELISA, DFA, flotationand lateral immuno - chromatography assay (Triage) respectively, P<0.05. Also statistical analysis reveals significance of PCR sensitivity, specificity, and accuracy in detecting giardiasis than other laboratory methods. Negative predictive values NPV in relation to type of laboratory methods are high, but statistically they are not significant, controversy to positive predictive value PPV that are significant. The efficacy of Triage panel is high for detecting giardiasis 14.45 % as compare with 4.5 % and 3.5 % for detecting Cryptosporidium parvum oocysts and Entamoeba histolytica respectively P<0.05 %. Considering the application of double direct wet preparations, the results of using this method are beneficial for detecting protozoan and helminthic parasites. While the using of zinc sulphate flotation technique reveal fluctuated results in spite of significant statistical analysis P<0.05. The employee of five laboratory methods for detecting the oocysts of Cryptosporidum parvum; the following rates 6.45%, 5.48%, 4.83%, 4.51% and 3.22% are recorded with the usage of DFA, direct microscopy, modified Ziehl - Neelsen method, Traige panel and flotation method respectively, P>0.05. Giardia lamblia DNA extraction from 80 stool samples that amplified using Giardia gene loci K725, reveal 66 samples positivity, pure Giardia lamblia genomic mass mean is 437.56 bps, with 1.705 % of genome purity and the extension of genomes range from 280 to750bps.While 23 of mixed Giardia plus other protozoan parasites, the mean of gemonic mass is 439.89 bps with genome purity mean 1.56 %. Amplification of Giardia genome mixed with helminthes reveal 443.33 bps of genomic mass mean and 1.49 % genome purity mean. In general the all genomic mass of Giardia lamblia (Pure and mixed infection) is 440.26 bps and purity mean 1.54 % P>0.05.PCR amplification in stool sample exert that Giardia lamblia genomes are mixed of human and animal type.

دراسة انتشار الحيوانات الابتدائية الرئيسية المسببة للاسهال بين المرضى باستخدام الطرائق المجهرية والجزيئية في محافظة بابل == Prevalence Study For Main Protozoa Diarrheal Agents Among Patients By Using Microscopically And Molecular Methods In Babylon Province

اسم المؤلف: ميس كاظم عليوي
اسم المشرف: احمد خضير عبيس الحميري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية خلال المدة من تشرين الاول 2014 الى شهر شباط 2015 حيث تضمنت هذه الدراسة فحص 987 عينة براز (اطفال وبالغين, ذكورو اناث) بطريقة الفحص المجهري المباشر و96 عينة موجبة فحصت عن طريق تقنية تفاعل سلسلة متعدد البلمرة. للمرضى المصابين بالاسهال و| The current study during period was conducted from October 2014 till February 2015 examination of 987 stool samples for direct smear method (Lugol's Iodine, Normal Saline (0.9%) for detection of the following parasites G.lamblia, E.histolytica while using floatation methods and Ziehl - Neelsen method (Malachite green) for detecting the Cryptosporidium spp. by using light microscope) and 96 positive samples from these samples examined by polymerase chain reaction technique PCR. For patients infected with diarrhea (children and adults, male and female) who attended to Babylon maternity and children hospital and specialized Marjan Hospital for Internal and Cardiac Diseases in the Babylon province as well as primary health care and private clinics. The age ranges from(Less than one year - 31and more).The current study showed the rate of infection with parasites that causative of diarrhea47.3% (E.histolytica, G.lamblia and Cryptosporidium spp.) was 26.4%, 17.9% and 3.7%, respectively.They were examined by a direct smear method to detect the trophozoites, cyst and oocyst phases of these parasites. The highest rate of infection by microscopic examination was in the rural area 67.2% in comparing with city that was 32.9%. Also the highest rate of infection among males was 51.5% in comparing with females 41.2%. It has been recorded that the higher rate of infection was 76.1% in the age group (16 - 20) years while the lower infection rate was in the age group (26 - 30) years that was 22.8%. It has been observed significant differences in infection rates at the (P ? 0.05). The present study recorded difference in the rates of parasitic infections according to the presence of animals in houses, the high rates of infection where with those have animals in their houses 51.2% while the lowest rates of infection where with those not have animals in their houses 38.9%.It has been observed significant differences in infection rates at the (P ? 0.05). This study reveals a difference in parasitic infections rates, according to the level of education the head of household, and found that the highest rate of infection for those with the head of the family is non - educated or illiterate 48.3%, while the rate of infection was declined with head of the family who get primary education level 46.2%. It had recorded the highest infection rate in October 63.8%, while the lowest percentage was in February 27. 6%. It has been observed significant differences in infection rates at the (P ? 0.05). In the present study Polymerase chain reaction (PCR) is used to 96 positive results in direct smear methods to detection the main parasitic diarrhea agent. It had recorded total of infection rate of 43.4% (31.3%, 28.1% and 2.1%, respectively). Depending on PCR technique showed the highest rates of infection was in the male 36.7% while the lowest rates of infection in females 30.6%. It had recorded the highest rate of infection in the rural areas 45.3% in comparing rate of infection in the urban areas 25.9%. The present study recorded the highest rates of infection were in the (16 - 20years) age group 46.2%, while the decline in the rates of infection was in the (21 - 25years) age group 16.7%. Also it showed the highest rates of infection were in the presence of animals in the houses 36.1% while the lowest rate of infection where there were no animals 31.4%.It has been observed significant differences in infection rates at the (P ? 0.05). The study found that the highest rates of infection for those with the head of the family is non - educated or illiterate 38.9%, while the rate of infection declined with head of the family who got Academic education level 33.3%. Also it had recorded the highest rate of infection in November (2014) 42.1%, while the lowest rates percentage was in December (2014) 23.1%. It has been observed significant differences in infection rates at the (P ? 0.05). Through the current study it was concluded that the prevalence of the parasites that cause diarrhea in the Babylon province are very highly when detection microscopic examination and PCR method, comparison with previous studies and rural area highest rates of infection from urban area.

تاثير المستخلص الكحولي لنبات الخباز (Malva sylvestris ) في فسلجة ونسجية الكبد والخصية في ذكور الفئران البيض == Effect of Alcoholic Extract of Mallow (Malva Sylvestris) On Physiology And Histology Liver And Testis In Albino Male Mice

اسم المؤلف: بسمة علي جاسم
اسم المشرف: مختار خميس محمد سعيد
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: صممت هذه الدراسة للتعرف على مدى تاثير المستخلص الكحولي الخام لنبات الخباز Malva Sylvestris في الكبد والخصى لذكور الفئران البيض نسجيا, وكذلك فسلجيامن خلال دراسة مستوى انزيمي Glutamate - oxaloactate - transaminase (GOT) وGlutamate - pyruvate - transaminase( | This study was designed to investigate the effects of crude alcoholic extract of Malva sylvestris on the histology of liver and testes in male albino mice, as well as levels of the GOT, GPT enzymes and testosterone hormone. Seventy two adult male albino mice were used in this study. The animals were randomly divided into four main groups, the first group was given distilled water (control), while the other three groups were given (100, 300, 500 mg /kg) of crude extract. These main groups were subdivided into three subgroups, according to the period of treatment (2, 4, 6, 8)weeks. Blood samples were collected every two weeks for physiological study, and samples of the liver and testes were collected for histological study. Treating with crude alcoholic of Malva sylvestris led to no significant increase in the mean of the body weight except the concentration (500 mg /kg). There was significantly increase in the liver weight particularly in(500 mg /kg) for eight weeks of exposure, also there was significantly increase in the average of the testes weights treated with Malva sylvestris extract specially in (300, 500 mg /kg) for all periods of treatment compared with control group. Malva sylvestris extract coused significantly decrease in both GOT, GPT. there fore the value of GOT, GPT considers as concentration - period exposure dependent the highest value of GPT was (300 mg /kg) for two weeks (2. 25±228. 04) IU/L compared with control group (3. 03±226. 02) IU/L. and the lowest value of GPT was (500 mg /kg) for eight weeks (4. 52±192. 27)IU/L compared with control group (4. 06±208. 77) IU/L the highest value of GOT in (100 mg /kg) reached (4. 10±63. 00) IU/L for two weeks compared with control group (1. 25±71. 23) IU/L. the lowest value was (2. 77±49. 83) IU/L in (500 mg /kg) with eight weeks comparison with control group (1. 18±69. 02) IU/L. all concentration of Malva sylvestris extract made positive changes in the value of testosterone with all periods of exposure particularly in (300 mg /kg) compared with control group. There were many histopathological alterations appeared in the liver tissue among treated groups which caused by the effects of Malva sylvestris extract represented by congestion, disarrangement of hepatic cords, beginning of necrosis of hepatocytes, infiltration of leukocytes, narrowing of sinusoids, increase the number of binuclei cells, degeneration, appearance of giant cells, all these changes were reversible. Malva sylvestris extract made beneficial histopathological changes in the testes tissue in all treated groups like increase in numbers of ledyig cells specially concentration (500 mg /kg) reached (11.37± 0.80) IU/L with two weeks comparison with control group(5.38 ±0.34) IU/L, and increase in the thickness of the walls of seminiferous tubules specially concentration (500 mg /kg) reached(29.46± 1.39) IU/L with compared with control group(20.52±2.40) IU/L. In addition the role of extract in significant decline in the diameters of seminiferous tubules in all treated groups compared with control group

دراسة العلاقة بين فقدان الجينين الـ GSTM1 وGSTT1 والاصابة بمرض الربو القصبي في محافظة ذي قار == Study The Relationship Between The Null Genotype of GSTM1, GSTT1 Genes And Bronchial Asthma In Thi - Qar Province

اسم المؤلف: نـور عمران عبد الكريم الغـزي
اسم المشرف: حسن ريسان الركابي | عدنان عيسى البدران
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ذي قار
الصفحات الاولى:
المستخلص: تم تصميم هذه الدراسة للتحقق من دور جينات الـ Glutathione - S - Transferase (Mu - 1) gene و(Theta) - 1 Glutathione - S - Transferase ومدى مساهمتها بخطر الاصابة بمرض الربو القصبي في محافظة ذي قار. حيث شملت (105) شخص مصاب بالربو باعمار من (20 - 69) سنة كان | This study has been designed to investigate the roles of the gens GSTMI and GSTTI the extent to which they contribute in asthma at Thi - Qar governorate. The study has been conducted on (105) patients with asthma whose ages from (20) to (69) years. (20) of them are males and (85) are females, and (110) are healthy with no infection of asthma or any sickness of respiratory system as a control group of the same ages, (60) of these are males and (50) are females. Blood samples have been collected from both groups in ampoules with EDTA and stored under temperature ( - 20) until they are used in getting the DNA from the groups. The genes GSTTI, GSTMI, and Albumin have been enlarged as internal control employing Polymerase chain Reaction (PCR). Some dangerous aspects of this disease have been investigated, the results showed that the disease in the urban areas (85. 71 %) higher than it rural areas, and Asthma among females is (80. 95%) higher than males. The results also indicated that asthma in ages (50 - 59) is more than other categories; it is (34.28%). The majority of patients are smokers with (63.80%). The study revealed that (73.33 %) of the patients have a family history with the disease while (26.66 %) have not. Statistical analysis showed that the loss of the gene GSTM1 is about three times in the patients more than the case in the control group. (OR=2.90) , while the loss of the gene GSTT1 is five fold more than it compared to the control group (OR= 5.55). The results indicated a significant effect of the loss of the two genes in having asthma in the urban and rural areas, it is six times and a half larger in the urban areas compared to the control group of the same area. Asthma probability is ten times more in the case of the loss of both genes in the rural areas compared to the control group in the same area. And no significant difference has been discovered when gene patterns of patients in both areas. The results also indicated that the probability of having asthma in male patients is two times and a half as many as it in the females when the gene GSTM1 is lost compared to the control group. While the same probability is eleven times in the case of the loss of both genes in the females as many as it the control group. The loss of Missing the gene GSTT1 is three times in the male patients and five times in the females compared to the two control groups. The results indicated significant differences when gene patterns of males and females are compared in the case of the loss of both genes. The statistical analysis indicated that the loss of both genes in the smoker patients contributes 17 fold in the probability of having the disease compared to the control group. While the probability increases four times approximately in the nonsmokers when the gene GSTM1 is lost compared to the control group (OR=3.77; 95% C1=1.69 - 8.407). No difference has been noticed in the gene patterns of the smoker and nonsmoker patients. The results indicated that the loss of both genes has been in all the age categories. No significant differences have been noticed among the gene patterns between the patients of less and those who are more than 50 years. Asthma probability has been found 7.77 times more in the case of the loss of both genes in the patients who have a family disease history compared with the control group. The asthma probability is twice when the gene GSTM1 is lost in those patients who do not have a family history disease compared to the control group. Upon comparing the gene patterns of patients according to the family disease history, there has been an effect of the loss of both genes and having a family disease history on the probability of asthma, it is almost six times when both genes are lost. The results indicated that the probability increases three times in the loss of the gene GSTT1 in the educated patients compared to control group. The probability also increases seven times when both genes are lost in the uneducated patients compared to the control group. When comparing the gene patterns of patients according to levels of education, significant differences between the educated an uneducated have been approximately twice in the loss of the gene GSTT1.

نوعية البويضة والتكوين الجنيني بعد الاعطاء الفموي للسبيرماكس لاناث الفئران : موديل تجريبي للبائن == Oocyte Quality And Embryonic Development After Oral Administration of Speramax® In Female Mice : Experimental Model For Mammal

اسم المؤلف: هبة صاحب حمزة
اسم المشرف: سعد صالح الدجيلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الاجنة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Background : Speramax® has been found to play an important role in sperm function characters and males reproductive performance with no studies on its effects on the oocyte maturation and embryonic development in females.Objective : The goal of the thesis is to examine the effect of Speramax ® on oocyte maturation. And to emphasize the effect of Speramax ® on ova quality, embryonic development and newborn. Materials and Methods : Speramax ® treated by oral administration for 1, 2 and 4 weeks. A hundred and twenty six female mice were randomly divided into four groups, the first group was treated without Speramax® with superovulation(SUO) while the second group were treated by Speramax® with SUO and the third group were superovulated only and the fourth group was not treated and spontaneously ovulated (SPO) and considered the control group(thirty two mice). Results : The results indicated that the treatment with Speramax® showed a positive effect on oocytes maturation in vivo. There was a highly significance (p? 0.0001) improvement in number of mature oocytes following treatment with Speramax® in SPO and SUO mice compared with SPO and SUO mice not treated with Speramax®. The embryonic developmental rate after 24 and 48 hours of mating in treated groups with Speramax® was significantly (p? 0.05) higher than those of SPO and SUO mice too.The study showed that the quantity and quality of embryos generated from the treated groups were superior to that of untreated groups.Conclusion : It was concluded that the treatment by Speramax® has a great improvement on oocyte maturation, early embryonic development and embryo grading quality of mice embryos with an increase in the numbers of mice newborn.

الفعالية المضادة للاحياء المجهرية والانزيمية للفطريات المستنبتة المعزولة من نبات الاس Myrtle (MyrtuscommunisL.) == Antimicrobial And Enzymaticactivity of Endophyticfungiisolatedfrommyrtle (Myrtuscommunisl.)

اسم المؤلف: هدى محمد كاظم ديوان
اسم المشرف: بتول زينل علي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: استهدفت الدراسة الحالية عزل وتشخيص الفطريات المستنبتة في اوراق نبات الاس Myrtus communis واختبار فعاليتها المضادة للاحياء المجهرية وفعاليتها الانزيمية. - اظهرت نتائج عزل الفطريات المستنبتة في 500 قطعة اوراق للحصول على عدد كلي للعزلات مساويا الى 99 عزلة ف | The present study was aimed to isolate and identify endophytic fungi from leaves of myrtle Myrtus communisand study their antimicrobial and enzymatic activities. - Results of fungal isolation from 500 leaves fragments revealed isolation of 99 endophytic fungi represented a total colonization frequency (CF) of 20.4%. These fungi were related to 23 species or isolates, where Aspergillus spp. prevailed over all others (11 species) with CF of 14% for all Aspergilli, Aspergillusniger and Aspergillusflavus showed the highest CF 7.2% and 3% respectively, other Aspergilli represented Aspergillusparvulus (CF 1.6%), Aspergilluscandidus (0.4%), Aspergillusraperi, Aspergillusclavatus, Aspergillusglaucus, Aspergillusornatus, Aspergillussclerotioniger, Aspergillusterreus and Aspergilluswentii were all showed CF of 0.2%.Other isolates fungi from leaves fragments were four morphologically different isolates of Penicillium with 0.4% CF for each. Additionaly, isolates of Cladosporium sp. (2%) and Cladosporiumcladosporoides (0.4%), as well as Cunninghamella sp., Drechsleraaustraliensis, Alternariaraphani, which showed 0.2% CF for each, Paecilomyces variotii (0.6|%), and different isolates of sterile fungi (White and Hyaline colonies) were also obtained. - Study the antagonistic activity of isolated endophytes using dual culture method against five plant pathogenic fungi(Fusarium oxysporum, Fusarium graminearum, Fusarium sp., Macrophomina phaseolinaandRhizoctonia solani)taking into account the percentage of inhibition of pathogenic fungi, results revealed variation of antagonistic activities between endophytes and pathogens. Different cases of antagonism were also appeared ranged from mycoparasitism of the endophytes on pathogens or vice versa, to competition between the two fungi and to the appearance of inhibition zone between them, this result may indicates the affinity of using some isolated endophytes in biological control applications against or controling fungal plant diseases. - Effect of ethylacetate extracts of culture broth of active endophytes shown in the last experiment against two bactria E.coli, S.aureus and the yeast Candida albicans using disc diffusion method, where filter paper discs were imprignated with different concentrations of fungal broth extracts. Results showed variation of inhibitory activity against bacteria with different endophytes, bacteria and conc. of the extract. Regarding the effect onC.albicans and filamentous fungi, most fungal broth extracts showed no inhibitory activity against yeast and no extract as well showed inhibitory activity on filamentous fungi. These results give indication thatbroth extracts contain antibacterial compounds. - Addition of 20% crude fungal broth of some endophytes to the culture medium showed significant inhibitory activity against filamentous fungi which did not respond to broth extracts, percentage of inhibition ranged between 0 - 77.08% and maximum inhibitory activity was shown by Penicillium sp. Isolate. These results give indication that some of crude filtrates contain antifungal compounds. - Analysis of broth extracts of some endophytes using TLC technique showed the content of extract of number of UV illuminated spots isolated on TLC pates having different Rf values, Bioautography showed inhibitory activity of some of these isolated spots against E.coli, S.aureus and the Filamentous fungus F.oxysporum. - Testing the extracellular enzymes activities of isolated endophyles on solid media, enzymes included amylase, lipase, pectinase, laccase, cellulase and protease showed variation in secretion of these enzymes, as well as, no endophytes showed the affinity of secreting all tested enzymes. Results also showed the high affinity of some endpohytes to secrete enzymes that could be use in multiple important applications after separation andcharacterizationof these enzymes.

دراسة مرضية فسلجية جزيئية لمرضى الثلاسيميا نوع بيتا في محافظة المثنى - العراق == Patho Physiological And Molecular Studies of ? - Thalassemia Patients In Al - Muthanna Province - Iraq

اسم المؤلف: هناء علي عزيز
اسم المشرف: خالد كاطع الفرطوسي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الفسلجة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: المثنى
الصفحات الاولى:
المستخلص: هدفت الدراسة الحالية لتقييم التغيرات الدمية والكيموحيوية للمرضى المصابين بالثلاسيميا نوع - ? وكذلك تحديد الطفرات المسببة للمرض بواسطة سلسلة تفاعل البوليمر ARMS - PCR ولاول مرة في محافظة المثنى - العراق. خلال الفترة من تشرين الاول - 2013 لغاية اذار - 2014. | The aim of this study was detected of hematological, biochemical changes and detection of mutations which cause ? - thalassemia by ARMS - PCR assay for the first time in Al - Muthanna province - Iraq, during the period from October - 2013 up to March - 2014. One hundred patients with thalassemia were examined in the present study as well as fifty apparently healthy people were selected as the control, their ages ranged between 2 - 20 years old, these patients were registered as thalassemic patients in "Thalassemia Unit" at "Feminine and Children Hospital" in Al - Muthanna province.The patients of ? - thalassemia were examined by using hematological and biochemical tests. The study recorded non significant differences at (P>0.05) in the infection percentage of male (56%) and female (44%) with thalassemia. The study showed a significant increase at (P<0.05) in the infection percentage of thalassemia in age groups, location, relative degree, blood group and infected viral hepatitis. The study indicated a significant decrease at (P<0.05) in red blood cells, hemoglobin and packed cell volume in all age groups compared with control groups. Also, the study showed a significant decrease at (P<0.05) in red blood cells of thalassemia between all age groups while it showed non significant differences at (P>0.05) in hemoglobin and packed cell volume of thalassemia between all age groups. The study showed a significant increase at (p<0.05) in platelets in age groups (1 - 5) years which was (356238+ 24244)U/L and (15 - 20) years was (278311+ 17640) U/L as compared with control groups (274000+84481) U/L and (216667+ 70384)U/L respectively. Also, the study recorded a significant increase at (P<0.05) in platelets of thalassemia in age group (1 - 5) years compared with other age groups. The study indicated a significant decrease at (P<0.05) in total white blood cells in all age groups as compared with control groups. Additionally, it showed a significant differences at (P<0.05) in total white blood cells of thalassemia between age groups.The study recorded a significant decrease at (P<0.05) in neutrophile in all age groups as compared with control groups. Moreover, it showed non significant differences at (P>0.05) in netrophile of thalassemia between all age groups. The study showed a significant decrease at (P<0.05) in basophile in age groups (1 - 5) years, (5 - 10) years and (15 - 20) years as compared with control groups. Furthermore, it showed a significant increase at (P<0.05) in basophile of thalassemia in age group (10 - 15) years as compared with other age groups. The study recorded a significant increase at (P<0.05) in eosinophile in all age groups as compared with control groups. Also, it showed non significant differences at (P>0.05) in eosinophile of thalassemia between all age groups. The study showed a significant increased at (P<0.05) in lymphocyte in age groups (1 - 5) years, (5 - 10) years and (10 - 15) years as compared with control groups. Also, it showed non significant differences at (P>0.05) in lymphocyte of thalassemia between all age groups. The study indicated a significant decrease at (P<0.05) in monocyte in age groups (5 - 10) years (1.094+ 0.093) % and (10 - 15) years (0.483+ 0.093) % as compared with control groups (5.166+ 1.359) and (5.824+ 1.555) respectively.Also, it showed a significant increase at (P<0.05) in monocyte of thalassemia between age groups. The study indicated a significant decrease at (P<0.05) in urea level in age groups(1 - 5) years (23.63+ 5.88) mg/dl and (5 - 10) years (26.86+ 6.45) mg/dl as compared with control group (35.37+7.90) mg/dl and (33.83+5.26) mg/dl. Inaddition, it recorded non significant differences (P>0.05) in urea of thalassemia between all age groups. The study showed a significant decrease at (P<0.05) in creatinine level in age groups (1 - 5) years, (10 - 15) years and (15 - 20) years as compared with control group. Also, it recorded non significant differences (P>0.05) in creatinine of thalassemia between all age groups. The study showed a significant increase at (P<0.05) in Alanine aminotransferase , bilirubin in all age groups as compared with control groups. Also, the study recorded non significant differences at (P>0.05) in Alanine aminotransferase of thalassemia between all age groups, while it showed significant increase (P <0.05) in bilirubin of thalassemia in age group (15 - 20) years as compared with age groups. The study indicated significant increase at (P<0.05) in Aspartate aminotransferase in age group (1 - 5) years (23.71+7.54)U/L as compared with control group (12.33+3.51) U/L. Also, it recorded non significan differences at (P>0.05) in Aspartate aminotransferase of thalassemia between all age groups. The study recorded a significant increase at (P<0.05) in concentration of ferritin in all age groups as compared with control groups. Also, it showed significant increase at (P <0.05) in ferritin of thalassemia in age group (15 - 20) years as compared with other age groups. The study showed non significant differences at (P>0.05) in concentration of uric acid and albumin in all age groups as compared with control groups. Also, it recorded non significant differences at (P>0.05) in uric acid and albumin of thalassemia between all age groups. The study indicated a significant decrease at (P<0.05) in total protein in age groups (1 - 5) years, (5 - 10) years and (10 - 15) years as compared with control groups. Also, it recorded non significant differences at (P>0.05) in total protein of thalassemia between all age groups. The study showed a significant decrease at (P<0.05) in calcium concentration in age groups (1 - 5) years, (10 - 15) years and (15 - 20) years as compared with control groups. Also, it recorded non significant differences at (P>0.05) in calcium concentration of thalassemia between all age groups. The present study diagnosed three types of mutation in ? - thalassemic patients by ARMS - PCR assay (IVS - I - 5, Codon 8\9, Codon15), the highest percent of ? - thalassemic patients mutation is IVS - I - 5 (53.8 %) followed by Codon 8\9 and Codon15 with percentage (27.6%) and (18.4 %) respectively

تقييم مستوى الثرومبوسبوندين لدى مرضى السكري النوع الثاني == Assessment of Thrombospondin Level In Diabetic Patients Type II

اسم المؤلف: وجدان راجح حمزة الكريطي
اسم المشرف: ارشد نوري غني الدجيلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: The present study is intended to asses serum levels of Thrombospondin - 1, Fasting blood glucose, Glycated Hemoglobin A1c, Lipid profile and BMI in type 2 diabetic patients, also correlation between Thrombospondin - 1 with all criteria above in both males and females.The study was conducted on randomly selected 65 type 2 diabetic patients (34 males and 31 females) attending the diabetes mellitus center in Al - Sadder Teaching City in Al - Najaf province, Iraq and a group of 24 apparently healthy subjects (12 Males and 12 Females) were included as a control group. The Study was carried out from August 2013 to February 2014.The patients' age was ranging of 35 to 64 years old.The results indicated a significant increase (p<0.05) in serum FBG, Cholesterol, TG, VLDL - C, LDL - C, TSP - 1 levels and a significant decrease (p>0.05) in serum HDL - C level in diabetic patients in comparing with healthy groups. The results also revealed that significant increase (p<0.05) in serum HbA1c level and BMI in diabetic patients in comparing with healthy groups.The results also revealed no significant differences (p> 0.05) in serum Cholesterol, Triglyceride, VLDL - C, LDL - C, HDL - C and TSP - 1 levels between males and females in both patients and health groups, while the results of FBG and HbA1c levels increase significantly (p<0.05) in females than males in both patients and health groups.The results show that Cholesterol, Triglyceride, VLDL - C, LDL - C, TSP - 1, FBG and HbA1c levels increase significantly (p<0.05) in both males and females patients groups in comparing with males and females of healthy groups respectively, and a significant decrease (p<0.05) in serum HDL - C in both males and females patients groups in comparing with males and females of healthy groups respectively.The results indicated a significant increase (p<0.05) in serum TSP - 1 level in females than males patients at same BMI (over weight and obese) except normal weight there is no significant differences (p>0.05) and there is no significant differences (p>0.05) between males and female of control groups at same BMI.The results show that TSP - 1 increase significantly (p<0.05) with increasing age of patients in males and females patients and the ages (55 - 64y) are highly significant(p<0.05) than (45 - 54y) and (35 - 44y), But there is no significant differences (p>0.05) in males and females of healthy groups at different ages. while there is a significant increase (p<0.05) in serum TSP - 1 level at same ages in both males and females patients in comparing with males and females of healthy groups respectively. The results also indicated that TSP - 1 increasing significantly (p<0.05) with increasing duration of disease in males and females patients and the (11 - 15y) reveals a highly significantly (P<0.05) than (6 - 10y) and (1 - 5y). The results have been shown significant positive correlation (P<0.05) between TSP - 1 and FBG, TSP - 1 and HbA1c, TSP - 1 and BMI, TSP - 1 and cholesterol, TSP - 1 and TG, TSP - 1 and LDL - C, TSP - 1 and VLDL - C in (males and females), males, females DM patients. The results have been shown significant negative correlation (P<0.05) between TSP - 1 and HDL - C in (males and females), males, females DM patients. The present study concluded that Thrombospondin - 1 and HbA1c levels were markers for detection and diagnosis of diabetic patients type

التحري عن نوعية مياه الشرب لمحطتي معالجة مياه الحي والبشائر في محافظة واسط جنوبي العراق == Investigation of Drinking Water Quality In Al - Haay And Al - Bashaar Water Treatment Plants In Wasit Province Southern Iraq

اسم المؤلف: وسام باسم محمد التميمي
اسم المشرف: احمد جاسم محمد العزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: م اجراء فحوصات فيزيائية وكيميائية واحيائية لمياه الشرب في محطتي الحي والبشائر وعدد من المناطق التي تغذيها, شهريا للمدة من تشرين الاول 2013 الى شهر تموز 2014, عبر مراحل التصفية وصولا الى المنازل التي تقع على مسافات مختلفة عن مصدر التجهيز وبواقع نموذجين لكل | Physical, chemical and a biological tests were carried out and drinking water samples were collected from AL - Haay and AL - Bashaer water treatment plants and number of residential areas fed by these plants for period extended from October 2013 to July 2014, through the purification stages up to residential sites at different areas situated with various distances from the supplying source, two samples were taken monthly. Air temperature at sampling time was varied from 16°C to 42°C and from 10°C to 34°C, for water temperature at sampling time also. The results of pH values were within the allowable limits, ranging from 7.1in autumn to 8.1 in summer. For EC, the highest value recorded for raw water was in winter with 1338 µs/cm, while the lowest value was in spring with 920 µs/cm. The highest value recorded for drinking water was again in winter with 1330 µs/cm, and the lowest value was 910µs/cm in spring also. The highest value of turbidity recorded for raw water was in winter with 89 NTU and the lowest value was in spring with 26 NTU, while the highest mean value recorded for drinking water was 20 NTU again in winter and the lowest value was 1 NTU in spring. The study showed that the results of TDS values ware ranged from 622 mg/L in spring to 1024mg/L in winter. The highest value of residual chlorine was recorded in summer with 4.5 mg/L; the lowest value was zero mg/L in some of the farthest points of the plants. For sulfates, values were ranged from 289 mg/L in spring to 498.4 mg/L in winter. All the recorded values of chlorides ions for all water samples were within the allowable limits, its values ranged from 98mg/L in summer to156.3 mg/L in winter. For total hardness, the values were exceeding 500mg/L and allowable limits for Iraqi standard criteria. The highest value recorded for raw water was in winter with 520 mg/L and the lowest value was in summer with 351 mg/L, while the highest value recorded for drinking water was again in winter with 516mg/L and the lowest value was 337 mg/L in summer also. For calcium values ranged from 75 mg/L in summer to 135 mg/L in winter. The results of this study showed high levels of lead, cadmium and aluminum in most water samples which collected during study period compared with those that exceeded the acceptable limits provided from the quality control unit and which are considered to have adverse effects on health. For lead, values were found to vary from 0.0030 mg/l in summer to 0.16 mg/l in winter (higher acceptable limits is 0.01 mg/L). While in case of cadmium the values lied between 0.0014 mg/l in winter and 0.015 mg/l in summer (higher acceptable range is 0.0030). However, for aluminum these data were ranged from 0.0062 mg/l in raw water during spring to 0.29 mg/l in drinking water during spring also (higher acceptable range is 0.2). The current results showed an increased in the number of autotrophic bacteria, total coliform, fecal coliform, fecal streptococci, and E. coli during winter season in most study locations as compared to those of the other seasons for both water plants raw and drinking water while the FS values were less than those of other bacterial types for both water plants. The Total plat count results of drinking water were exceed 100 cell/ml, the allowable limit for drinking water, for some samples of both water plants. on the other hand, the TC, FC and E. coli exceeded zero cell/100ml, the allowable limits for drinking water, in many drinking water samples for both water plants
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