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دراسة مرضية فسلجية جزيئية لمرضى الثلاسيميا نوع بيتا في محافظة المثنى - العراق == Patho Physiological And Molecular Studies of ? - Thalassemia Patients In Al - Muthanna Province - Iraq
Author name:
هناء علي عزيز
Supervisor name:
خالد كاطع الفرطوسي
General topic:
Biology
Specific topic:
Zoology - Physiology
Degree:
Master
University:
Al-Muthanna University - College Of Science - Department Of Biology
Language:
English
University location:
Muthanna
First pages:
24T2675 - p.pdf
Abstract:
هدفت الدراسة الحالية لتقييم التغيرات الدمية والكيموحيوية للمرضى المصابين بالثلاسيميا نوع - ? وكذلك تحديد الطفرات المسببة للمرض بواسطة سلسلة تفاعل البوليمر ARMS - PCR ولاول مرة في محافظة المثنى - العراق. خلال الفترة من تشرين الاول - 2013 لغاية اذار - 2014. | The aim of this study was detected of hematological, biochemical changes and detection of mutations which cause ? - thalassemia by ARMS - PCR assay for the first time in Al - Muthanna province - Iraq, during the period from October - 2013 up to March - 2014. One hundred patients with thalassemia were examined in the present study as well as fifty apparently healthy people were selected as the control, their ages ranged between 2 - 20 years old, these patients were registered as thalassemic patients in "Thalassemia Unit" at "Feminine and Children Hospital" in Al - Muthanna province.The patients of ? - thalassemia were examined by using hematological and biochemical tests. The study recorded non significant differences at (P>0.05) in the infection percentage of male (56%) and female (44%) with thalassemia. The study showed a significant increase at (P<0.05) in the infection percentage of thalassemia in age groups, location, relative degree, blood group and infected viral hepatitis. The study indicated a significant decrease at (P<0.05) in red blood cells, hemoglobin and packed cell volume in all age groups compared with control groups. Also, the study showed a significant decrease at (P<0.05) in red blood cells of thalassemia between all age groups while it showed non significant differences at (P>0.05) in hemoglobin and packed cell volume of thalassemia between all age groups. The study showed a significant increase at (p<0.05) in platelets in age groups (1 - 5) years which was (356238+ 24244)U/L and (15 - 20) years was (278311+ 17640) U/L as compared with control groups (274000+84481) U/L and (216667+ 70384)U/L respectively. Also, the study recorded a significant increase at (P<0.05) in platelets of thalassemia in age group (1 - 5) years compared with other age groups. The study indicated a significant decrease at (P<0.05) in total white blood cells in all age groups as compared with control groups. Additionally, it showed a significant differences at (P<0.05) in total white blood cells of thalassemia between age groups.The study recorded a significant decrease at (P<0.05) in neutrophile in all age groups as compared with control groups. Moreover, it showed non significant differences at (P>0.05) in netrophile of thalassemia between all age groups. The study showed a significant decrease at (P<0.05) in basophile in age groups (1 - 5) years, (5 - 10) years and (15 - 20) years as compared with control groups. Furthermore, it showed a significant increase at (P<0.05) in basophile of thalassemia in age group (10 - 15) years as compared with other age groups. The study recorded a significant increase at (P<0.05) in eosinophile in all age groups as compared with control groups. Also, it showed non significant differences at (P>0.05) in eosinophile of thalassemia between all age groups. The study showed a significant increased at (P<0.05) in lymphocyte in age groups (1 - 5) years, (5 - 10) years and (10 - 15) years as compared with control groups. Also, it showed non significant differences at (P>0.05) in lymphocyte of thalassemia between all age groups. The study indicated a significant decrease at (P<0.05) in monocyte in age groups (5 - 10) years (1.094+ 0.093) % and (10 - 15) years (0.483+ 0.093) % as compared with control groups (5.166+ 1.359) and (5.824+ 1.555) respectively.Also, it showed a significant increase at (P<0.05) in monocyte of thalassemia between age groups. The study indicated a significant decrease at (P<0.05) in urea level in age groups(1 - 5) years (23.63+ 5.88) mg/dl and (5 - 10) years (26.86+ 6.45) mg/dl as compared with control group (35.37+7.90) mg/dl and (33.83+5.26) mg/dl. Inaddition, it recorded non significant differences (P>0.05) in urea of thalassemia between all age groups. The study showed a significant decrease at (P<0.05) in creatinine level in age groups (1 - 5) years, (10 - 15) years and (15 - 20) years as compared with control group. Also, it recorded non significant differences (P>0.05) in creatinine of thalassemia between all age groups. The study showed a significant increase at (P<0.05) in Alanine aminotransferase , bilirubin in all age groups as compared with control groups. Also, the study recorded non significant differences at (P>0.05) in Alanine aminotransferase of thalassemia between all age groups, while it showed significant increase (P <0.05) in bilirubin of thalassemia in age group (15 - 20) years as compared with age groups. The study indicated significant increase at (P<0.05) in Aspartate aminotransferase in age group (1 - 5) years (23.71+7.54)U/L as compared with control group (12.33+3.51) U/L. Also, it recorded non significan differences at (P>0.05) in Aspartate aminotransferase of thalassemia between all age groups. The study recorded a significant increase at (P<0.05) in concentration of ferritin in all age groups as compared with control groups. Also, it showed significant increase at (P <0.05) in ferritin of thalassemia in age group (15 - 20) years as compared with other age groups. The study showed non significant differences at (P>0.05) in concentration of uric acid and albumin in all age groups as compared with control groups. Also, it recorded non significant differences at (P>0.05) in uric acid and albumin of thalassemia between all age groups. The study indicated a significant decrease at (P<0.05) in total protein in age groups (1 - 5) years, (5 - 10) years and (10 - 15) years as compared with control groups. Also, it recorded non significant differences at (P>0.05) in total protein of thalassemia between all age groups. The study showed a significant decrease at (P<0.05) in calcium concentration in age groups (1 - 5) years, (10 - 15) years and (15 - 20) years as compared with control groups. Also, it recorded non significant differences at (P>0.05) in calcium concentration of thalassemia between all age groups. The present study diagnosed three types of mutation in ? - thalassemic patients by ARMS - PCR assay (IVS - I - 5, Codon 8\9, Codon15), the highest percent of ? - thalassemic patients mutation is IVS - I - 5 (53.8 %) followed by Codon 8\9 and Codon15 with percentage (27.6%) and (18.4 %) respectively
