البحث — المستودع الرقمي العراقي

المستخلص: اجريت? هذه الدراسة لمعرفة تاثير بعض الاضافات في الاستجابة المناعية والوزن والتغيرات النسجية في الامعاء الدقيقة. حيث تم تقسيم (350) طيرا من فروج اللحم بعمر يوم واحد من نوع Ross 308) ) غير مجنسة , وزعت عشوائيا الى (7) معاملات تغذوية متساوية وهذه المعامل | This study was conducted to investigate the effects of feed additives on immune response , weight and histological changes in small intestine. A total of (350) broiler chicks one - day old (Ross 380) - unsexed were randomly divided in to (7) equal dietary treatment groups , as fallows : Treatment (T1) : Basal diet without any addition ( control group).Treatment (T2) : Basal diet + Garlic 4cc/10 LTreatment (T3) : Basal diet + Saccharomyces cervisiae 2.5gm/10 L.Treatment (T4) : Basal diet +Stpire 2.5cc/10 L.Treatment (T5) : Basal diet +B. glucan 8gm/10 kgTreatment (T6) : Basal diet + Levamisole 1gm/kg.Treatment (T7) : Basal diet + Neomycin 0.5 gm/L.These additions were presented on the first day of the arrival for 5 days, and on the day of first vaccination for 5 days and at the end of experiment for 5 days also. This chicks vaccinated with NDV on the day one of age by injection in the neck and re - vaccinated at day 10 of age with LaSota, Body weight were weekly determined. ,blood samples were collected from wing vein for ELISA test in ( 4,18,27 ) days old respectively. The results as following : - 1 - Treatment of levamisole (T6) were significantly increased ( p<0.05) in (T2, T5) in level of Abs production against ND virus.2 - Chicks feed diet with saccharomyces cerevisiae showed significantly increased ( p<0.05) in body weight among all experimental group , and improvement in weight in (T6,T7) with other group.3 - Chicks feed diet with (T7) showed improvement in villus hight and in the ( T3, T4).

دراسة التاثير العلاجي لحليب الافراس المخمر للتقليل من تاثير الاجهاد التاكسدي المستحدث بواسطة خلات الرصاص في الجرذان البيض == Study The Therapeutic Effect of Fermented Mare? S Milk To Reduce The Effect of Oxidative Stress Induced By Lead Acetate In White Albino Rats

اسم المؤلف: شيماء عباس صبيح

اسم المشرف: كريم ناصر طاهر

الموضوع العام: الطب البيطري

السنة: 2015

الموضوع الدقيق: الصحة العامة

الدرجة: ماجستير

الجامعة: جامعة القادسية - كلية الطب البيطري

اللغة: العربية

مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: اجريت الدراسة الحالية في البيت الحيواني التابع لكلية الطب البيطري / جامعة القادسية بهدف تقييم الدور العلاجي لحليب الافراس المخمر في التقليل من تاثير الاجهاد التاكسدي المستحدث بواسطة خلات الرصاص. استخدم في هذه الدراسة ( 40 ) ذكر من الجرذان البيضاء نوع ( w | The present study was conducted at the animal house of veterinary medicine college of AL - Qadisiyah University during the period from 27 - 2 - 2014 to 30 - 3 - 2014. to evaluate the productive role of fermented mare's milk in to reduce the oxidative stress that induced by lead acetate , 40 male albino rats weighing between 200 - 210 gm were used in this study. the animals were obtained from the animal house of veterinary medicine College / Baghdad University. The animals were randomly distributed into four equal groups , ( 10 rats per each group / cage) as follow : - Control group : The rats were given only basal diet and deionized drinking water - - First treatment group ( T1) : The rats were given basal diet and deionized drinking water that contain 1000 ppm lead acetate for 30 days. - Second treatment group ( T2 ) : The rats were administrated basal diet and deionized drinking water that contained 1000 ppm of lead acetate and received orally ( by gavag ) fermented mare's milk at a dose ( 10ml / kg b.w / day ) for 30 days. - Third treatment group (T3) : The rats were given basal diet and deionized drinking water that contained 1000 ppm of lead acetate and received orally ( by gavag ) EDTA at a dose ( 50 mg/ kg b.w / day ) for 30 days. At the end of the experiment all the animals were fasted for ( 12 hours ) and then killed animals from each treatment group blood samples were collected from the heart to measures some blood parameters such RBC , Hb and PCV and biochemical parameters such as liver function parameters ( total protein and activity of some enzymes such as ALT , AST , ALP and LDH ) , kidney function parameters such as Urea and Creatinine concentration and oxidative stress parameters such as GSH , SOD , MDA ). In addition to that study the histopathological changes of liver and kidney tissues were studied The results of the present study can be summarized as follow : - 1 - Blood parameters : The results of the statistical analysis showed that there was a significant ( p < 0.05 ) decrease in the mean values of RBC , Hb , and PCV in a group treated with lead acetate only ( T1 ) as compared to other experimental groups. while groups treated with EDTA and fermented mares milk showed a significant ( p < 0.05 ) improvement in blood parameters. 2 - Biochemical parameters : A - Liver function parameters : The result revealed that there was a significant ( p < 0.05 )effect of lead acetate on level of total protein where caused a decrease in total protein concentration and increase the in activity of ALT , AST , ALP , and LDH as compared to other experimental groups. Yet treatment with fermented mare's milk ( T2 ) resulted in an increase in the total protein concentration and a decrease in the activity ALT , AST , ALP AND LDH. Also treatment with EDTA ( T3 ) caused a similar results to ( T2 ). B - Kidney function parameters : The results obtained in this study demonstrated that a significant ( p < 0.05 ) increase in the level of urea and creatinine in a group of animals treated with lead acetate only ( T1) , while using fermented mare's milk ( T2 ) and also EDTA contributed reduction in the level of urea and creatinine 3 - Oxidative stress parameters : The results of the present study showed that there was a significant ( p < 0.05) decrease in the concentration of GSH and SOD a significant ( p < 0.05 ) increase in MDA concentration in animals that administrated lead acetate only ( T1) while administration fermented mare's milk and EDTA resulted in significant ( p < 0.05 ) increase in the concentration of GSH and SOD while a significant ( p < 0.05 ) reduction in MDA concentration. The values becomes close to the concentration of the control group. 4 - Histopathological changes : The results of Histopathological examination for tissues of liver and kidney of rats given lead acetate alone ( T1 ) showed that there was a clear Histopathological changes presented in degeneration of hepatocytes and congestion in central vein which associated with hemorrhage in liver and kidney tissues of animals administrated fermented mare's milk showed normal and free from congestion.

عزل وتشخيص جزيئي لمورثات مجاميع الانماط المصلية لجرثومة اللستيريا المستوحدة Listeria monocytogenes من الانسان والحيوانات في محافظة القادسية == Isolation And Molecular Detection of Serotype Genes Group of Listeria Monocytogenes From Human And Animals In Al - Qadissyia Province

اسم المؤلف: حسين عمران كريم العابدي

اسم المشرف: هدى عبد الهادي علي النصراوي

الموضوع العام: الطب البيطري

السنة: 2015

الموضوع الدقيق: الامراض المشتركة

الدرجة: ماجستير

الجامعة: جامعة القادسية - كلية الطب البيطري

اللغة: العربية

مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: اجريت الدراسة الحالية خلال الفترة من تشرين الثاني 2014 الى نيسان 2015، جمعت العينات المرضية من النساء التي تعاني من حالات الاجهاض وولادة مبكرة والاطفال المصابين بالتهاب السحايا من مستشفى الولادة والاطفال التعليمي وقد جمعت العينات تحت اشراف طبي متخصص وكان | This study was carried out during the period from November 2014 to April 2015, whereas samples were collected from women that suffered of abortion and infected children in the maternal and children hospital in Al - Qadissiya province , so the samples were collected under supervising of a specialist medicine , the number of taken samples of the women is 65 samples , while the number of taken samples of children is 32 samples , so the samples that belong to animals have included a gall bladder samples were collected from Al - Diwaniya slaughterhouse were 100 samples of sheep and 100 samples of cows. in addition to collect 200 samples of milk as 100 samples for each sheep and cows from different rural zones in al - Qadissiya province. L.monocytogenes was isolated by using oxford listeria selective agar for listeria , so the special stimulator for growth of the bacteria was added to it in order to determine the type of bacteria , also it diagnosed an isolates for L.monocytogenes by using the microscopically examination and biochemical tests , furthermore the germ isolates was planted on blood agar for diagnosing the blood analysis type : ? - heamolysis as affirmative checking for the isolates. also PCR technique used for affirming the diagnosing of L. monocytogenes by using the initiator for the blood analysis gene (hly) , whereas all the L. monocytogenes isolates' gave a positive result in inspection , so this technique is used too in the detection about the serous types for L.monocytogenes isolates by the molecular diagnosing for the fierce genes of the germ and these are (D1,D2,Glt,FlaA and MAMA gene ). The results of the research showed that isolating and diagnosing percentage of the Listeria monocytogenes is about 9.27%, whereas the isolation percentage in women infected in abortion is 4.61%, while the percentage of listeria germ isolation was higher in the children that infected in meningitis whereas it was 18.75% of the total amount of the samples.As for the percentage of the isolating in sheep it was 4% and 7% for milk and gall bladder respectively. In the cows the isolation percentage of the germ from milk and gall bladder was 2% and 3% respectively. So the the results of isolating for listeria germ during the study period in human and animals were disparate , whereas the highest percentage of isolation for the germ in human were scored in January and February months of the year whereas it scored 20% and 16% respectively.As for sheep the percentage of isolation was higher in December and January months whereas it is 6.25% and 10% respectively , while in the cows the percentage was higher in January and February months where it is 4.2% and 7.4% respectively. Regarding The results of detection about the genes of the serous types for the isolates of L.monocytogenes by using PCR technique 14 isolates of the samples showed a positive result when they react with the initiator for gene (D1)in percentage about 56%, so this isolates showed a positive result when they react with the initiator for gene (Glt) as amount of 5 isolates and they classified to be belong to the serous types (1/2B or 3B), while 9 isolates gave a negative result in the inspection , whereas these isolates were tested with the initiator for gene (MAMA), 5 isolates showed a positive result in this reaction with this gene and they classified to be belong to the serous types (4c or 4a), while 4 isolates gave a negative result as they classified to be belong to the serous types (4b or 4d or 4c).As for the isolates' that gave a negative result with the initiator of gene (D1)there are 11 isolates all these isolates gave a positive result when they inspected with the initiator of gene (D2) in a percentage about 44%, so when these isolates inspected with the initiator for gene (FLaA) then 6 isolates of them gave a positive result in the inspection and classified as they belong to (1/2a or 3a), while 5 isolates of them gave a negative result in the inspection and classified as they belong to (1/2c or 3c). As for the serous types that have not diagnosed in the germ isolates of the human there are(1/2a or 3c), while in the germ isolates of the sheep the serous types (4a or 4c) have not been diagnosed , then the serous types (1/2b or 3b) have not been scored in the cows. The present study concluded that the percentage of infection in L.monocytogenes was high in human in al - Qadissiya province , in which that refer to the risks of the diseases and health problems spread that could be caused by L.monocytogenes , also the results included isolating the germ from milk and gall bladder samples for sheep and cows that could be a source to carry the infection to the human by contaminating the meat, milk and its derivatives'. whereas the isolation percentage of the germ was higher in the cold months of the year in human and animals, so the highest isolation percentage of the serous types for the L.monocytogenes in human and cows were (4a , 4c) , while in sheep the serous types were (1/3c,3c).

استخدام حليب الافراس المخمر في علاج قرحة المعدة المستحدثة بالاسبرين في اناث الجرذان البيضاء == Usage of Fermented mareP,Ps Milk In Treatment of Aspirin Induced Ulcer In Female Albino Rats

اسم المؤلف: اسراء طاهر مسلم العبيدي

اسم المشرف: كريم ناصر طاهر | علي محمد غازي

الموضوع العام: الطب البيطري

السنة: 2015

الموضوع الدقيق: الصحة العامة

الدرجة: ماجستير

الجامعة: جامعة القادسية - كلية الطب البيطري

اللغة: العربية

مكان الجامعة: القادسية

الصفحات الاولى:

المستخلص: This study was carried out at the animal house of veterinary medicine College /AL - Qadisiya university during the period from 27 - 6 - 2014 to 27 - 3 2014 the study aimed to investigate the effect of usage fermented mareP P s milk on the healing of gastric ulcer induced by aspirin. A total of 56 female albino rats weighing between 200 - 250g were used in this study.. The animals were divided randomly in to four equal groups each groups comprising of 14 females per each group.Untreated group or negative control groups (C) : were received standard diet and distilled water only.treated groups : - positive control group (T1), second treated group ( T2) and Third treated group (T3). All animals of these groups were induced ulcer by administrated orally(100mg/kg b.w ) aspirin drug for one week ,Then two animals from each group were sacrificed after 7 days to ensure that ulcer induced,after that the animals of second treatment group(T2) were given orally by gavage fermented mare P Ps milk at dose(10ml/kg b.w) once daily for three weeks consecutive while animals of third treatment group (T3) were treated oraly by gavage rantidine (50mg/kg b.w) once daily for three consecutive weeks, four animals per each group were sacrificed at the end of each week، and the stomach were excised to measures the ulcer parameters (ulcers score, ulcer index,ulcerinhibition percentage and PH of gastric juice value) blood samples were collected from all animals from the heart for blood test parameters (red blood cell count , hemoglobin concentration and packed cell volume).Biochemicals parameters such as alanine trans amines(ALT), aspartate trans amines(AST),alkaline phosphatase (ALP) total protein ,urea and creatinine and oxidative stress parameters such as malondialdehyde (MDA), superoxide dismutase(SOD),glutathione, (GSH), and prostaglandin (PGER2R).in addition tohistological examination to ensure the exist of ulcer. The results of this study revealed the following : ulcer parameters : the result of this study revealed that administration of aspirin produced formation multi - ulcer in the stomach wall besides causes different changes in the studied parameters al so, the result has been shown a significantly ( P<0.05) increase in the ulcer score and ulcer index in aspirin administration groups (T1)in Comparison with other experimental groups during the three weeks of the experiment on the other hand aspirin administration causes a significant (P<0.05) reduction in pH value of gastric juice , at the same time given fermented mare ,s milk improvement for the previous parameters and causes a significant (P<0.05) decrease in it along the three weeks of experiment as well as causes a significant (P<0.05) increase in ulcer inhibition ( %) and the pH value of gastric juice ,these values were closed to the values of ranitidine treated group (T3) and control groups respectively.Blood parameters : the result of the present study revealed that there was a significant ( P<0.05) decrease in the total count of RBC ,Hb concentration and PCV in aspirin administration group (T1) as compared to the other experimental group along the three weeks of experiment. while given fermented mar P Ps milk causes improvement in the previous parameters and produce a significant (P<0.05) increase of these parameters and became close to the values of rantidine groups and negative control groups.Biochemicals parameters : The result indicated that administration of aspirin produced a significant (P<0.05) increase in ALT,ASTand ALP activity as compared to the othe experimental groups but causes a significant (P<0.05) decrease in concentration of total protein particularly in the third weeks of experiment ,while giving fermented mare P,Ps milk causes improvement the previousparameters and causes a significantly (P<0.05) reduction in it and became close to the values of rantidine groups and negative control groupsOxidative stress parameters : the result of the present study revealed that there was a significantly( P<0.05) increase in (MDA concentration) and significantly( P<0.05) decrease in the concentration of (SOD,GSH and PGE2) in aspirin treated groups ,while giving fermented mare ,s milk causes a significantly( P<0.05) decrease in concentration of MDA and a significantly (P<0.05) increase in the concentration of( SOD ,GSH ,PGE2) these result are closed to the result of rantidine treated groups and negative control group.the result also shown there were non significant differences in concentration of urea and creatinine along experimental weeks among different groups ,on the other hand, the results of the histological examination of aspirin treated groups that a pathological changes in the stomach tissue represented in formation ulcers , congestion and bleeding.while giving fermented mare's milk contributes in the active form in hide of ulcers in the stomach wall, hemorrhage signs and congestion similar results obtaining when treated with rantidine.it could be concluded that aspirin administration causes negative effect on stomach also the study has been showed the important role of fermented mare's milk in reduction damages and negative effects of aspirin.