استخدام حليب الافراس المخمر في علاج قرحة المعدة المستحدثة بالاسبرين في اناث الجرذان البيضاء == Usage of Fermented mareP,Ps Milk In Treatment of Aspirin Induced Ulcer In Female Albino Rats
Author name:
اسراء طاهر مسلم العبيدي
Supervisor name:
كريم ناصر طاهر | علي محمد غازي
General topic:
Veterinary Medicine
Specific topic:
Public Health
Degree:
Master
University:
University of Al-Qadisiyah - College Of Veterinary Medicine
Language:
Arabic
University location:
Qadisiyah
First pages:
21T498 - p.pdf
Abstract:
This study was carried out at the animal house of veterinary medicine College /AL - Qadisiya university during the period from 27 - 6 - 2014 to 27 - 3 2014 the study aimed to investigate the effect of usage fermented mareP P s milk on the healing of gastric ulcer induced by aspirin. A total of 56 female albino rats weighing between 200 - 250g were used in this study.. The animals were divided randomly in to four equal groups each groups comprising of 14 females per each group.Untreated group or negative control groups (C) : were received standard diet and distilled water only.treated groups : - positive control group (T1), second treated group ( T2) and Third treated group (T3). All animals of these groups were induced ulcer by administrated orally(100mg/kg b.w ) aspirin drug for one week ,Then two animals from each group were sacrificed after 7 days to ensure that ulcer induced,after that the animals of second treatment group(T2) were given orally by gavage fermented mare P Ps milk at dose(10ml/kg b.w) once daily for three weeks consecutive while animals of third treatment group (T3) were treated oraly by gavage rantidine (50mg/kg b.w) once daily for three consecutive weeks, four animals per each group were sacrificed at the end of each week، and the stomach were excised to measures the ulcer parameters (ulcers score, ulcer index,ulcerinhibition percentage and PH of gastric juice value) blood samples were collected from all animals from the heart for blood test parameters (red blood cell count , hemoglobin concentration and packed cell volume).Biochemicals parameters such as alanine trans amines(ALT), aspartate trans amines(AST),alkaline phosphatase (ALP) total protein ,urea and creatinine and oxidative stress parameters such as malondialdehyde (MDA), superoxide dismutase(SOD),glutathione, (GSH), and prostaglandin (PGER2R).in addition tohistological examination to ensure the exist of ulcer. The results of this study revealed the following : ulcer parameters : the result of this study revealed that administration of aspirin produced formation multi - ulcer in the stomach wall besides causes different changes in the studied parameters al so, the result has been shown a significantly ( P<0.05) increase in the ulcer score and ulcer index in aspirin administration groups (T1)in Comparison with other experimental groups during the three weeks of the experiment on the other hand aspirin administration causes a significant (P<0.05) reduction in pH value of gastric juice , at the same time given fermented mare ,s milk improvement for the previous parameters and causes a significant (P<0.05) decrease in it along the three weeks of experiment as well as causes a significant (P<0.05) increase in ulcer inhibition ( %) and the pH value of gastric juice ,these values were closed to the values of ranitidine treated group (T3) and control groups respectively.Blood parameters : the result of the present study revealed that there was a significant ( P<0.05) decrease in the total count of RBC ,Hb concentration and PCV in aspirin administration group (T1) as compared to the other experimental group along the three weeks of experiment. while given fermented mar P Ps milk causes improvement in the previous parameters and produce a significant (P<0.05) increase of these parameters and became close to the values of rantidine groups and negative control groups.Biochemicals parameters : The result indicated that administration of aspirin produced a significant (P<0.05) increase in ALT,ASTand ALP activity as compared to the othe experimental groups but causes a significant (P<0.05) decrease in concentration of total protein particularly in the third weeks of experiment ,while giving fermented mare P,Ps milk causes improvement the previousparameters and causes a significantly (P<0.05) reduction in it and became close to the values of rantidine groups and negative control groupsOxidative stress parameters : the result of the present study revealed that there was a significantly( P<0.05) increase in (MDA concentration) and significantly( P<0.05) decrease in the concentration of (SOD,GSH and PGE2) in aspirin treated groups ,while giving fermented mare ,s milk causes a significantly( P<0.05) decrease in concentration of MDA and a significantly (P<0.05) increase in the concentration of( SOD ,GSH ,PGE2) these result are closed to the result of rantidine treated groups and negative control group.the result also shown there were non significant differences in concentration of urea and creatinine along experimental weeks among different groups ,on the other hand, the results of the histological examination of aspirin treated groups that a pathological changes in the stomach tissue represented in formation ulcers , congestion and bleeding.while giving fermented mare's milk contributes in the active form in hide of ulcers in the stomach wall, hemorrhage signs and congestion similar results obtaining when treated with rantidine.it could be concluded that aspirin administration causes negative effect on stomach also the study has been showed the important role of fermented mare's milk in reduction damages and negative effects of aspirin.
