عرض: 25 50 75 100 النتائج

نتائج البحث: 25 من أصل 1,882

التتابع الجيني لطفيليات Entamoeba histolytica, E. dispar and E. Moshkoviskii في محافظة القادسية == Phylogenetic Sequeincing For Spesies Entamoeba Histolytica, E. Dispar, E. Moshkoviskii In Al - Qadisiya Province

اسم المؤلف: هبة رياض جميل العبودي
اسم المشرف: هادي مدلول حمزة الميالي
الموضوع العام: علوم الحياة
السنة: 2015
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة للمدة من بداية اذار 2014 والى نهاية ايلول 2014 وشملت على مراجعين مستشفى الديوانية العام ومستشفى النسائية والاطفال في محافظة القادسية ومختبرات التحليل الاهلية في المحافظة, وقد تم فحص679 عينة براز باستخدام طريقة المسحة المباشرة لمختلف الفئ | This study has been conducted for the period from the first of March 2014 until September 2014 and included the auditors to Diwaniya General Hospital and Women's Hospital and children in the province of Diwaniya and civil analytical laboratories in the province. A sample of 679 of auditors' stool have been examined by using the direct swab from different age groups and both sexes. The study shows that 61.26% of people have been infected by screened amoeba parasite. Real time PCR was used in this study to diagnose Amoeba species in 200 samples. Result shows that Entamoeba histolytica had highest percentage of incident followed by Entamoeba dispar then Entamoeba moshkoviskii, which were 74 %, 26 %, and 7 % respectively.Statistical level of ?=0.05presents significant differences between then. Percentage of positive molecular test samples was higher than the negative ones; they were 88% and 12%. Molecular diagnoses were used in this study. E. histolytica had highest individual incident followed by E.disbar then E. moshkoviskii : 56% 10%, and 1% respectively. Using significant level of 0.05, significant differences were appeared. Rates of mixed infections of E. histolytica + E. dispar followed by E. moshkoviskii + histolytica then E. moshkoviskii + E. dispar, which were 14%, 4%, and 2% respectively. Statistics shows significant differences between them at ? =0.05. Symptoms of individual incident of the three Amoeba sorts were observed and recorded. Patients who had E. histolytica, severed from acute diarrhea that ranged between mucous and watery texture, which its color contrast between brown and reddish brown. They had bloody diarrhea and abdominal pain that ranged from severe to moderate colic. They had also slight temperature increase and weight loss. For those who had E.disbar, abdominal pain and frequent pale yellow diarrhea that semi - aqueous texture were recorded. Symptoms of : moderate yellowish brown snotty diarrhea, abdominal pain and, weight loss were observed for those who had E. moshkoviskii. R.T.PCR (Taqman Probe) was used to isolate samples and to investigate the three pathogenic virulence genes, which are Cystiene Proteinase, Amebopore, and Gal/Gal Nac Lectin. This was done in each of Amoeba’s species using R.T.PCR (Syber Green). According to it, E. histolytica possesses Cystiene Proteinase 1, Amebopore, and Gal/Gal Nac Lectin at 86.6%, 80.35%, and 42.8% respectively. Non - significant differences were found at ? =0.05 between them. For E.disbar, result shows existence of the same pathogenic virulence genes at percentages of 65%, 45%, and 20% respectively. Result presents significant differences among percentage of genes isolation for the pathogenic virulence factors at ? =0.05. E. moshkoviskii had these three pathogenic virulence genes at percentage of 33.3% by isolating the virulence genes except Gal/Gal Nac Lactin, there were significant differences among them at ? =0.05. This study was conducted to understand the molecular characterization confirmatory of the three types of insulators parasite using traditional enzyme chain polymerize reaction as a first stage to investigate the 18s ribosomal RNA covenantal. It represents personal heritage of the three types of amoeba. Depending on the same gene, developed tree were analyzed for Amoeba’s species using method of Neighber - Joining among different groups of the stains for each species separately using MEGA6 program. Knowing convergence rates of the used stains and international isolators from NCBI, Blast program was used. Since there is no study has been done to study nitrogenous bases sequence for these three species, samples undertook molecular characterization confirmatory was registered in the national data base center (NCBI). International serial numbers were obtained and attached to the dissertation.

اليات استحثاث المقاومة في نبات الخيار بواسطة Trichoderma harzianum وBacillus subtilis وGlomus mosseae اتجاه الفطر Rhizoctonia solani المسبب لمرض سقوط البادرات == Resistance Mechanisms In Cucumber Plant By Trichoderma Harzianum, Bacillus Subtilis And Glomus Mosseae Against Rhizoctonia Solani Causing Seedling Disease

اسم المؤلف: رباب مجيد عبد الذهيبي
اسم المشرف: علي هاشم الموسوي | هادي مهدي عبود
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: نفذت هذه الدراسة للتحري عن اليات استحثاث المقاومة في نبات الخيار ضد الفطر الممرض Rhizoctonia solani بوساطة المعاملة بالعوامل الاحيائية وهي harzianum Trichodermaو Bacillus subtilis وGlomus mosseae وتاثير ذلك في نمو نبات الخيار وحاصله النامي تحت ظروف البيت | This study was carried out to investigate the mechanisms of induced resistance in cucumber plant against pathogenic fungus Rhizoctonia solani by bioeagent factors Tricoderma harzianum fungus, Basillus sibtilis bacteria and mycorrhizal fungus Glomus mosseae. The effect in parameters growth and yield of cucumber plant which growing under greenhouse and field conditions. The pathogenic fungus showed high Pathogenesis on cucumber plant. Three isolated of B. subtilis and T. harzianum showed high antagonism ability against R. solani under laboratory condition. For mycorrhizal fungus G. mosseae isolated was tested to study their ability in cucumber growth for chosen the best isolated which can used in anther experiment which results were as follows : - A - Pots experiment 1 - The bioeagent factors was showed their ability to induced several mechanisms of resistance in cucumber plant against pathogenic fungus R. solani, Which included increasing the activity of defense enzymes in existed of pathogenic fungus R. solani, The treatment (T. harzianum + G. mosseae) was recorded highest level in enzyme activity of Phenylalanine ammonia layase enzyme which was (25.3541) unit/gm fresh weight, While T. harzianum treatment showed significantly increased the enzyme activity of Polyphenol oxidase and Peroxidase which was (10.6707, 2.6137) unit/gm fresh weight respectively. This study showed no enzyme activity for chitinase enzyme excepted in some treatment and some periods.This study recorded increased in plant content of total phenol, lignin, salicylic acid hormone, total protein, The (G. mosseae + B. subtilis) treatment overtaken on other treatments in increased plant content of total phenol which was (1.989) mg/gm fresh weight, B. subtilis treatment was significantly increased plant content of lignin which was (18.318) mg/gm fresh weight, The treatment (T.harzianum+ B. subtilis) showed significantly increased plant content of salicylic acid hormone which was (1.8658) mg/gm fresh weight. This study recorded no Jasmonic acid hormone activity in treated plant except in some treatments but with low level, The treatment (G. mosseae + T.harzianum + B. subtilis) was recorded highest content in total protein which was (239.3) ?g/mg. 2 - The rate of seedling death and disease index was significantly reduced in bioeagent treatments, The lowest rate for seedling death in the treatment of (G. mosseae + B. subtilis), (G. mosseae + T.harzianum) and (G. mosseae + T.harzianum + B. subtilis) which were 11 % each for them comparison with control treatmentwith pathogen which was 100%.3 - The rate number of spores and percentage of mycorrhiza infection was significantly increased when mycorrhizal fungus G. mosseae interaction with T. harzianum or B. subtilis. The treatment of mycorrhiza infection with pathogen recorded highest number of mycorrhizal spores which was (1410) spore. The mycorrhiza infection reach to 100% in (G. mosseae + T.harzianum) and(G. mosseae + T.harzianum + B. subtilis) treatment with pathogen fungus. The treatment of (G. mosseae + T.harzianum) without pathogen recorded highest index of mycorrhiza infection which reach to (80)%, But the index of mycorrhiza infection and mycorrhizal root weight were decreased in the existed of pathogenic fungus, Mycorrhizal dependency it was increased in all treatments with pathogenic fungus and the treatment of (G. mosseae + B. subtilis) with pathogen recorded highest percentage in Mycorrhizal dependency which was (58.873)%. 4 - The plant content of chlorophyll (a, b, total) was significantly increased, The tratment of (G. mosseae + B. subtilis) with pathogen recorded highest level in chlorophyll a it was (5.30)mg/gm, the highest level in chlorophyll b found in(G. mosseae + T.harzianum + B. subtilis) without pathogen it was (6.07)mg/gm, (G. mosseae + T.harzianum + B. subtilis) treatment without pathogen recorded highest level in total chlorophyll it was (10.79)mg/gm. The concentration of phosphor concentration increased in shoot part of (G. mosseae + T.harzianum) without pathogen which was (0.538)%, potassium concentration were decreased in all treatments with pathogen excepted (G. mosseae + T.harzianum) treatment, The highest level of potassium in treatment of (B. subtilis) without pathogen which recorded (4.65)%, For nitrogen concentration was increased in all treatments with pathogen and the highest level in (T.harzianum) which was (5.114)%. Carbon concentration significantly decreased in all treatments with pathogen and (G. mosseae + B. subtilis) without pathogen recorded highest concentration in carbon which was (46.203)%. 5 - The result showed that all growth parameters either in the absence or presence of pathogenic fungus was significantly increased in all bioagent treatments.The percentage of germination reach to (100)% in (B. subtilis) with or without pathogen , the treatment (G. mosseae + B. subtilis) with pathogen recorded highest level in shoot length it was (92.40)cm, And the same treatment recorded highest rate in fresh and dry weight for shoot part which was (7.412, 0.990) gm respectively, (T.harzianum) without pathogen recorded highest rate in leaf area it was (453.083)cm2, The highest rate of flower number was in (T.harzianum) with pathogen which was (15.66)flower/plant. The fresh and dry weight for root, root length, root surface area and the rate of root diameter were significantly increased in comparison with control treatment without pathogen. The treatment of (G. mosseae + T.harzianum) without pathogen recorded highest level in fresh and dry weight for root which were (0.359, 0.094)gm respectively. Highest root length in (G. mosseae + B. subtilis) without pathogen it was (48.14)cm, root surface area decreased in the treatments with pathogen and the treatment (G. mosseae + T.harzianum + B. subtilis) without pathogen recorded highest root surface area it was (19.16)cm 2, The root diameter was increased in(G. mosseae) with pathogen was reach to (0.363)mm.B - Field experiment 1 - Under field condition the percentage of germination increased in the treatment without pathogen which were (94.44)% in treatments (G. mosseae) , (B. subtilis) and (G. mosseae + T.harzianum) respectively, In the treatments with pathogen the percentage of germination reduced and The bioeagent factors showed no reduction in percentage of disease infection or disease index with pathogenic fungi R. solani under field condition which reach to (100)%. 2 - The rate number of spores, percentage of mycorrhiza infection, index of mycorrhiza infection, mycorrhizal root weight and Mycorrhizal dependency on cucumber plant significantly increased when mycorrhizal fungus G. mosseae interaction with T. harzianum or B. subtilis. (G. mosseae + T.harzianum + B. subtilis) significantly overtaken on other treatments in increased rate of mycorrhizal spors it was (3950)spore, The mycorrhiza infection reach to (100)% in all treatments, For index of mycorrhiza infection the treatments of (G. mosseae + B. subtilis) and (G. mosseae + T.harzianum) recorded highest rate which reach to (100)%, (G. mosseae + T.harzianum + B. subtilis) treatment recorded high increased in mycorrhizal root weight and Mycorrhizal dependency which were (545.67 gm, 67.78%) respectively. 3 - The plant content of total chlorophyll was significantly increased, The highest level in(G. mosseae + T.harzianum + B. subtilis) treatment which was 5.19 mg/gm. There was significantly increased in the concentration of nutrition elements P, K, N, C in cucumber plant. The treatment (T.harzianum+ B. subtilis) recorded highest concentration in phosphor and nitrogen which were (489.4, 3.62) % respectively, The treatment of (G. mosseae + T.harzianum + B. subtilis) recorded highest concentration of potassium which was (2.975)%. The highest concentration of carbon recorded in B. subtilis treatment which was (34.823) %. 4 - All shoot, root and yield parameters of cucumber were significantly increased under field conditions. The treatment (T.harzianum+ B. subtilis) recorded significantly increased in plant height, dry shoot weight, leaf area rate number of flowers, rate number of fruit and fruit weight which were (42.87 cm, 10.85 gm, 631.06 cm2, 19, 17, 1275 gm) respectively. The treatment G. mosseae recorded significantly increased in fresh shoot weight which was (32.76) gm. The treatment with (T.harzianum+ B. subtilis) recorded significantly increased in fresh and dry weight of root, root length, root surface area, rate of root diameter, rate number of fruit and fruit weight which were (11.4 gm, 5.5 gm, 151.71 cm, 44.05 cm2, 0.763 mm, 17, 1275 gm) respectively,

تحمل التراكيب الوراثية من الذرة الصفراء Zea mays L. للاجهاد المائي بتاثير البرولين == The Tolerance of Maize (Zea Mays L.) Genotypes To The Water Stress Under Proline Influence

اسم المؤلف: قيود ثعبان يوسف الاسدي
اسم المشرف: عبد عون هاشم علوان الغانمي | عبد الجاسم محيسن جاسم الجبوري
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة في حقل خاص لمنطقة البركة (30) كم شمال شرق مدينة كربلاء اثناء موسم النمو 2012للعروتين الربيعية والخريفية باستعمال الاصص البلاستيكية. هدفت الدراسة معرفة تاثير الاجهاد المائي والرش بحامض البرولين وتداخلاتها في المؤشرات المظهرية والفسلجية و| This study was conducted in a private field at Albargah district (30Kms) northern east of Karbala city during spring and fall of 2012 growing season using plastic pots. The goal was assess the effect of water stress, foliar application of proline and their interactions on characteristics morphological, physiological and nutritional status of three genotypes of maize namely : Serour, 5018, and Baghdad - 3.The characteristics included root's volume, root's length, root's diameter, root's dry weight, plant height, leaves number, leaf area, shoot dry weight, root/shoot ratio, absolute and relative growth rates and content of chlorophyll and proline in the leaves. The nutritional status included, the concentration, the contents and the rates of absorption and transport of N, P, K, Ca and Mg.In addition to the molecular study which included the technique of Random Amplified Polymorphic DNA (RAPD) DNA. A Factional experiment within Completely Randomized Design (C.R.D.) was adopted i.e. three genotypes of maize namely : Serour, 5018, and Baghdad - 3, proline (0, 50, 100 and 150) mg. L - 1 and two levels of field capacity (50 and 100) % with three replicates in a total of 72 experimental units.Means were compared using the least significant difference (LSD) at 0.05 probability level. Results could be summarized as follow : 1 - Genotypes significantly differed in the studied traits.The cultivar 5018 gave the highest values of root's volume, root's length, root's diameter, root's dry weight, plant height, and root/shoot ratio for spring and fall cultivations.The same cultivar gave higher values of P%, Ca%, Mg% in roots and the content of N, P, K, Ca and Mg in roots, rates of absorption and transport of N, P and Ca in spring and fall cultivations.Leaves P%, Ca% and Mg %, leaves Mg content and absorption and transport of Mg in fall cultivation. on the other hand, Serour cultivar give higher values of leaves number, leaf area, shoot dry weight, absolute and relative growth rates, content of chlorophyll and N% in root in spring and fall cultivation. P% in leaves in fall cultivation, leaves K% in both cultivation, content of N, P, K and Ca of shoot in both cultivations, shoot's Mg content of shoot in fall cultivation, content of N, P, K and Ca of shoot in both cultivation and Mg content of shoot in fall cultivations. Baghdad - 3 cultivar give higher values of proline content, Mg% in roots in both cultivations, shoot Mg% in fall cultivations, absorption and transport rates of K in fall cultivation, transport rate of Ca in spring and fall cultivations and absorption and transport rates of Mg in fall cultivation. 2 - Apart from Ca% of root in spring and fall cultivation, the proline markedly influenced all the rest traits where 100 mg. L - 1 proline gave the highest values compared to control treatment (i.e. 0 proline) that gave the lowest values. The proline at 50 and 150 mg. L - 1 gave intermediate values for all studied traits. 3 - Apart from some cases, 100% field capacity treatment gave higher values of most studied parameters. The field capacity of 50% gave higher values of N% and K% in roots and leaves, the content of N and K of roots and shoots and rates of absorption and transport rates of K in spring and fall cultivations. on the other hand, there was no effect of field capacity on Ca% of roots and shoots in spring and fall cultivation and Mg% of roots in spring cultivations. 4 - Bi and Tri interactions effect was profound on most studied characteristics. 5 - Through molecular study that including isolation of DNA in leaves of maize , a quantity of DNA ranging between 150 - 290 nano - gram / ?l with 1.8 - 1.9 purity was obtained. The differentiation of (RAPD) DNA was analyzed in order to prepare the finger print of maize genotypes. Six primers were tested (OPA - 09, OPA - 11, OPA - 13, OPC - 12, OPD - 20 and OPN - 16). The primers revealed different multiplication products between genotypes.The least value of genetic distance was 0.4103 between 5018 and Baghdad - 3genotypes, while the highest value of genetic distance was 0.7932 between Serour and 5018 genotypes.Data analysis revealed that these genotypes are divided into two main groups namely : group included 5018 and Baghdad - 3 genotypes, and group included Serour genotype only.

تاثير بعض شدد المجبل المغنبطيسي المستقر في بعض المتغيرات الدمية والكيموحيوية والنسجية للفئران البيض Balb - C - albino Effect of Static Magnetic Fields In Some Physiological And Histological Aspects In Mice == Effect of Static Magnetic Fields In Some Physiological And Histological Aspects In Mice

اسم المؤلف: نضال ابراهيم لطيف الحداد
اسم المشرف: عبد علي ذاكر | عماد عبد الرحمن محمد صالح
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الانسجة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: الانبار
الصفحات الاولى:
المستخلص: Study includes following up of measuring the total dissolved protein and enzymes in different body organs and the structural changes (Histological)occurring in the testis and epididymis and liver and kidney of the male mice, in addition, the study includes following up of must the hematological and Hormones changes.This study consists of three experiments.The first experiment involved exposing male mice directly for thirty consecutive day to three different magnetic stressed 750, 1500, 3000 Gauss The second experiment involved exposing pregnant mothers directly during pregnancy, amounting to 21 consecutive days for the same intensites and after the end of exposure and the occurrence of birth was taking male embryos left until puberty to conduct the study by the third experiment involved SMF same intensites also for a period of 30 days after the end of the period of exposure was that males paired with females and left until the birth then was taking male embryos left until adulthood to conduct the study at the end of each experiment and weighed the animals and took blood samples and explained and eradicated all organs (liver, kidney the heart and spleen) for the first test and (liver and kidney) for the second and third as the experience was detected changes in the total dissolved proteins amount were observed using the method of Biuret and those on protein band resolved on polyacrylamide electrophoresis PAGE.Also the effect of SMFs on the activity of each of the enzymes ACP, ALP, ALT and AST was estinmated using the Spectroscopic method. Electrophoresis was used to observe effects on enzymic bands of Esterase on PAGE. In addition to biochemical variables included enzymatic antioxidants, which included enzymatic SOD, CATand MDA as has been the follow - up blood changes which included the amount of hemoglobin Hb, PCV, WBC, RBC and platelet counts and blood Were estimated as the concentration of each of the hormones (prolactin, LH, FSH) and testosterone hormone as eradicated all of the right testis and epididymic for the first experiment and excised portions of the liver and kidney for the second and third experiment and they were used to prepare histological sections for the histological studies.The tissues were sectioned and stained with Haematoxylin and Eosin stain using the routine histological techniques.The result of the study could summarized as follows : - - The Results of this study showed was an increase not significant (p?0.05) in body weight It also showed decrease not significant (p?0.05) In the liver and spleen weight either kidney, heart weight did not change in any weights appears when 750 and 1500 gauss either at 3000 gauss intensity was significantly increased and clear (P?0.05) compared with control animals.—There was a significant in (MDA, AST, ALT, ACP, ALK, TP) To extract the liver, kidney, heart and spleen male mice exposed to three different stressed 3000, 1500, 750 gauss As for the enzymes(CAT, SOD) Showed a significantdecrease in heart, kidney, spleen extract to mice exposed and rise significantly in the liver of mice exposed to stressed above the first experiment, there was no significant increase in the activity of enzymes (ACP, ALK, TP) And an increase in the activity of moral enzymes(MDA, SOD, CAT, AST, ALT) In liver extract and kidney male mice second experiment compared with control animals. The third experiment, there was no significant increase in the activity of enzymes (ACP, ALK, TP) And an increase in the activity of moral enzymes(MDA, SOD, CAT, AST, ALT) Liver extract in male mice third experiment compared with the control animals in either extract kidney male mice third experiment, there was not significant increase in (SOD, CAT, AST, ALT, ACP, ALK, TP) And a significant increase in the activity of the enzyme (MDA compared with control animals - The results showed some changes in the intensity and number of protein bands to extract four organs first experience at three 3000, 1500, 750 gauss compared with animal control and extract the liver and kidney for the second test and third at all intensities when electrophoresis was carried out on PAGE. - The results showed that there are some changes in the intensity and number of enzyme bands esteras in the extract of the four organs of the first experiment at 3000, 1500, 750 gauss compared with animal control and extract the liver and kidney for the second test and third at all intensities when electrophoresis was carried out on PAGE. - Results of the first experiment showed significant increase (P?0.05) in each of the(Hb, PCV, PLT and RBC) Animals exposed to either 1500.750 gauss at 3000 gauss intensity rise was not significant (P?0.05) compared with control animals. The results showed a significant decrease (P?0.05) in WBC at all intensities compared with control animals at all intensities.Results of the second and third experiment showed not significant increase (RBC, PCV, Hb and PLT) and not significant (P? 0.05) in WBC at all intensities compared with control animals at all intensities. - The results of the first experiment showed that there was significantly higher (P?0.05) in the LH hormone when exposed to the three stressed 3000, 1500, 750 gauss compared with control animals. there was a significant decrease (P?0.05) in each of the hormonal FSH prolactin When the exposed to the three stressed 3000, 1500, 750 gauss compared with control animals, but the Testosteron hormone has decreased at 750 gauss a significant decrease (P?0.05) compared with control animals. - The results of the first experiment not significant decrease (P?0.05) in the testis and epididymis weight of the animals exposed to 1500 and 750 gauss compared with control animals either at 3000 gauss intensity, there was a significant increase (P?0.05) in both organs weight compared with control animals. Histological studies showed the significant decrease in the diameter and thickness of epithelial tissue of the seminiferous tubules and epididymis tubules and duct efferentes inthe 750and 1500 Gauss/where appeared where appeared in epithelial tissue is incomplete and thinner than in the animal control either when the intensity of 3000 gauss there was a significant decrease diameters and high padded her cells and appeared in epithelial tissue integrated compared with animals control. - Histological study showed the liver of male experience the second occurrence of some histological changes in the central vein and semi - sinus blood and prepare Kupffer cells and epithelial cells lining compared with control animals. And the lack of any histological changes in male mice kidney second experiment and stressed 3000, 1500, 750 gauss compared with control animals.Histological study also showed the liver third male mice experiment that no histologic changes at 3000, 1500, 750 gauss compared with control animals either the kidney of male mice have shown some changes in some histological structures at 750, 1500 and 3000 Gauss, compared with control animals

استجابة ثلاثة اصـناف من حنطة الخبز Triticum aestivum L. باضافة سماد NPKZn والرش بالجبرلين == Response of Three Bread Wheat (Triticum Aestivum L.) 3With Gasprying Fertilizer And NPKZn Cultivars To

اسم المؤلف: عبد الجبار جاسم جريان
اسم المشرف: عباس جاسم حسين الساعدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: A field study was conducted at the farm of Biological Dpartment, College of Education (Ibn Al - Haitham) University of Baghdad during the grown season 2013 - 2014, to study the effect of compound fertilizer NPKZn (15 : 15 : 15 : 1) - Turkish origin was introduced to Iraq recently - in both ) 3(GA acid Gibberellicvarious concentrations of with morphological and chemical indicators, the components of yield, some quality and biological characteristics of three varieties of the Iraqi wheat (Bohoth 22, Alfath, Bohoth158). Experiment was carried out according to the design of complete sectors of randomization [Randomized Complete Block Design (RCBD)].160) (0 of fertilizer, two levels used experiment Asthree replicates, with ) 015, 100, acid concentrations (25, 50 Gibberellicof fourand 1 - .hKg and three varieties of as well as to the treatment of comparison 1 - .lmg wheat. So the expriement was factorial on (2 x 5 x 3).It included 90 experimental units..The study of components of yield have gone on its : 1. There is difference in these three different varieties Bohoth 22, Alfath, and Bohoth 158 in the studied indicators.The class Bohoth 158 exceeds on the other categories in the following phenotypic indicators (dry weight of vegetative part, space of flag leaf and sustainability of biomass), with increase as like {(17.85, 11.37), (14.66, 7.33) and (17.02, 14.24)}%, respectively, while Bohoth 158 significantly exceeds on the other varieties absolute growth rate. And also the differences were significant between varieties in the following chemical indicators (phosphorus and calcium content and the percentage of protein in the grain) and in the components of yield there was significant increase in the (spike length with awn and without its, the number of spikes, weight of spike and its grains, weight of grains in spike and grains yield), while in the Bohoth 158 there was a significant increase in qualitative and rheological characteristics for the following qualities, (the proportion of wet and dry Glutine, flour absorption susceptibility of water, sedimentation values, ash content in coarse bran compared to others two classes. 2. The results of the study showed that the transactions under the influence of fertilizer has excelled significantly in all the study indicators (morphological and chemical components of yield, quality and rheological) with the exception of the dry weight of roots. acid was the best among Gibberellic 1 - .lmgof 100 concentration 3. The the concentrations giving him the highest average of indicators of morphological and physiological components of yield, quality and rheological. and spray 1 - 160 Kg.hbetween the manure level overlap 4. The result Gibberelllic acid showed 1 - mg.l 100 of concentration under the significant increase in all studied indicators except for the (dry weight of roots, the sustainability of biomass, and 1000 - grain weight) 5.The trio overlap transactions showed exceeding of Bohoth 158 nder the influence u 1 - .hKg160 in level fertilizer of add ithw significantly 22 Bohothcategories : other acid onGibberellic of 1 - .lmgof spraying 100 and Alfath in the following indicators : Dry weightof the flag leaf, spike length with awn, number of spikes, grains yield, the percentage of dry Glutine and the values of sedimentation.

دراسة بيئية لمجتمع الطحالب وبعض الملوثات البيئية في نهر الحسينية - كربلاء المقدسة / العراق == Ecological Study For Algal Community And Some Pollutants In Al - Husseinia River Holy Karbala - Iraq

اسم المؤلف: سارا حمود عبد الامير الاسدي
اسم المشرف: فكرت مجيد حسن | جاسم محمد سلمان
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الاحياء المائية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:
المستخلص: اجريت دراســة بيئية شاملة على مياه نهر الحسينية في الفترة الممتدة مـن تشــرين الاول 2012 ولغاية ايلول 2013، تم فيها دراسة الخصائص الفيزيائية والكيميائية لمياه النهر بالاضافة الى اجراء دراسة كمية ونوعية لمجتمع الطحالب الهائمة والملتصقة المتواجدة في المناطق | Acomprehensive ecological study was conducted on Al - Husseinia River during the period from October 2012 to Septemper 2013. This study includes measuring some chemical and physical properties of water and conduct quantitive and qualitative study of the Algal community which includes the phytoplankton and the periphyton algae , in addition to measuring some of pollutants types such as Heavy metals and Polycyclic Aromatic Hydrocarbons (PAHs) the water of the river and its sediments and some kinds of aquatic plants such as Ceratophyllum demersum, Hydrilla verticillta, Potamogeton pectinatus and Phragmites australis. The results of the present study showed temporal and spatial variations in the physical - chemical properties of water. The water temperature ranged between (12.7 - 31.3) °C which accompanied the variations of air temperature that ranged between (8.5 - 45.3) °C. The river was tending to the alkalinity and the PH was ranged between 7.5 - 8.8 were dominated by bicarbonate ions, the electrical conductivity and the Total Dissolved Solids and The Total suspended solids ranged between (840 - 1416.67) ?s/cm and (413.33 - 700) mg / l and (6.33 - 55.07) mg / l respectively. The river was oligosaline with a salinity ranged between (0.54 - 0.91) PSU. The water current velocity and light penetration recorded fluctuated during the period of the study, their values ranged between (11.68 - 37.77) cm / Sec and (33.67 - 148.67) cm, respectively. The Dissolved oxygen and biological oxygen demand increased during the cold months and decreased during the warm months where the values ranged between (4.97 - 12.63) mg / l and (0.4 - 4.48) mg / l respectively. The river was very hard, the total hardness ranged between (240 - 516.67) mg / l, and the Total alkalinity ranged between (106.33 - 162) mg / l. Calcium ions and Magnesium ions ranged between (40.08 - 162.32) mg / l and (13.67 - 64.73) mg / l. The values of sulfates ranged between (58.94 - 131.37) mg / l ; their values show an increase at the study sites which were under highly human activities, but they did not exceed the permissible levels of the drinking water quality. The nutrients such as silica and total nitrogen and total phosphorous showed variations during the study and their values ranged between (1.63 - 6.26) mg/l and (1.4 - 3.5) mg / l and (1.33 - 17.29) ?g/l, respectively. A total of 303 algal taxa of phytoplankton and periphyton was identified during the study belonged to six classes of Algae; they include Bacillariophyceae (196 taxa) , Chlorophyceae (59 taxa) , Cyanophyceae (33 taxa) , Euglenophyceae (6 taxa) , Dinophyceae (6 taxa) and Cryptophyceae (3 taxa). The Bacillariophyceae were a dominated class in the phytoplankton community, they constitute 62.4 % of the total number of species followed by Chlorophyceae by 20.66 % and Cyanophyceae by 11. 6 % respectively. The Dinophyceae and Euglenophyceae constitute 2.07 %, while the cryptophyceae constitute the lower percentage which amounted to 1.2 % of the total species. Some identified algae like as Nitzschia, Navicula, Cymbella, Fragilaria, Scenedesmus and Oscillatoria were dominated by their species numbers in the phytoplankton community , and the total numbers of the phytoplankton cells ranged between (446.8 × 10 3 - 9177 × 10 3) cell / l. In October recorded the highest number of the cells, while in March recorded the lower numbers of the algal cells during the study. A total of 203 taxa was characterized in the epipelic algal community dominated by Bacillariophyceae by 79. 3 %, followed by Cyanophyceae by 10.84 % and Chlorophyceae by 6.9 % respectively, while the Euglenophyceae and Dinophyceae constitutes the lower percentage formed 1.48 % of the total species. The total number of epipelic algal cells ranged between (9924.5 to 10.02 × 104) cells / cm2. Winter season recorded the highest average of the total number of cells, while Spring season recorded the lowest numbers of cells. Also, some genera such as Nitzschia, Navicula, Cymbella, Fragilaria and Oscillaroria were dominated in the study period. During this study some taxa of epiphytic algae have also identified on four types of aquatic plants, which included C.demersum , H.verticillata, P.pectinatus and P.australis, the numbers of algal taxa on each there were 124, 116, 77 and 114 respectively. The results showed dominated of Bacillariophyceae followed by Chlorophyceae and Cyanophyceae and other classes respectively. Some algal genera are dominated by their species of the studied plants such as Cymbella, Navicula, Nitzschia, Fragilaria and Gomphonema. In Autumn the highest numbers of epiphytic cells were recorded, where the total numbers of cells in these plants are 74.24 × 104 cells/g, 38.46 × 104 cells/g, 19.22 × 104 cells/g and 38.88 × 104 cells/g respectively. While the lower number of cells recorded during Summer in each of C.demersum by 8.07 × 104 cells/g and H.verticillata by 8.034× 104 cells/g and P. pectinatus by 3.74 × 104 cells/g, whereas the P. Australis recorded the lowest number of cells during Spring which was 22.51 × 104 cells/g. C.demersum contained the highest numbers of algal cells compared to other plants. The distribution and concentrations of some Heavy metals such as Cd, Cu, Pb, Fe and Zn are studied in each of the water (dissolved and particulate fractions), and sediments (exchangeable and residual fractions) and four aquatic plants. The average of these heavy metals concentrations in the dissolved phase of the water were (1.4, 1.15, 3.74, 5.46 and 7.66) ?g / l, respectively. While their concentrations in the particulate phase were (3.67, 110.23, 63.97, 732.98 and 167.2) ?g/g respectively. In the sediments the concentrations of these heavy metals in the exchangeable phase were (2.53, 38, 43.45, 169 and 42.16) ?g/g, respectively, whereas their concentrations in the residual phase were (2.54, 32.82, 50.33, 1452.75 and 53) ?g/g, respectively. The concentrations of the Cd, Cu, Pb, Fe, Zn in the aquatic plants are also measured during this study and the average of theirs in C.demersum were (11.09, 14.16, 48.58, 2202 and 65.42) ?g/g, respectively. While their concentrations in the H. verticillate were (10.17, 12.99, 43.58, 2921.98 and 60.45) ?g/g, respectively. In P. pectinatus were (11.58, 13.52, 50.06, 2315.47 and 77.88) ?g/g, respectively. Whereas their concentrations in P.australis were (9.29, 11.83, 39.84, 1845.21 and 52.76) ?g/g, respectively. The distribution of sixteen Polycyclic Aromatic Hydrocarbons is also determined in Al - Husseinia River during this study in each of water, sediments and aquatic plants. These organic compounds include Naphthalene، Acenaphthylene، Acenaphthene، Fluorene، Phenanthrene، Anthracene، Fluoranthene، Pyrene، Benzo(a)Anthracene، Chrysene، Benzo(b)Fluoranthene، Benzo(k)Fluoranthene، Benzo(a)Pyrene، Dibenzo(a, h)Anthracene, Benzo(ghi)perylene وIndo(1, 2, 3 - cd)Pyrene. Their concentrations in the water of river ranged between (0.24 - 58.72) ng/l recorded for each of Benzo(a)Pyrene and Benzo(ghi)perylene respectively, the total concentration of PAHs in the water was 152 ng/L, the results also showed that the source of water pollution of these compounds was pyrogenic and petrogenic origin. In the sediments the concentration of these compounds ranged between (0.36 - 119.06) ?g/g. For each of Naphthalene and Benzo(ghi)perylene, respectively. The total concentration of PAHs in the sediment was 257.71 ?g/gm., The results also showed that the source of sediment pollution with these compounds was also pyrogenic and petrogenic origin as the water. In aquatic plants, the concentration of these compounds in C.demersum and H.verticillata ranged between (0.12 - 152.3) ?g/g and (0.3 - 129.8) ?g/g for Fluoranthene and Benzo(ghi)perylene to each plants respectively , while their concentration in P.pectinatus ranged between (0.25 - 99.36) ?g/g for each Benzo(b)Fluoranthene and Benzo(ghi)perylene respectively, in P.australis PAHs concentration ranged between (0.37 - 134.85) ?g/g for Chrysene and Benzo(ghi)perylene, respectively. C.demersum recorded the highest total concentration of PAHs compared to other plants, and the results showed that the source of pollution in these plants was Pyrogenic origin. They also revealed that Benzo(ghi)perylene recorded the highest concentration during this study compared with other compounds.

دور الكاينيتين والسماد المركب (NPKZn) في تحمل نبات الفلفل Capsicum annuum L.)) للاجهاد الملحي == Role of The Kinetin And NPKZn In Salt Stress Tolerance of Sweet Pepper (Capsicum Annuum L.)

اسم المؤلف: سعاد عبد سيد الجلالي
اسم المشرف: عباس جاسم حسين الساعدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت تجربة اصص في الحديقة النباتية التابعة لقسم علوم الحياة، كلية التربية للعلوم الصرفة - ابن الهيثم، جامعة بغداد في الموسم الربيعي عام 2014 هدف الدراسة كان دراسة تاثير تركيزين من كلوريد الصوديوم هي 50) و100) مليمول.لتر - 1 فضلا عن معاملة السيطرة واربعة | The aim of the pots experiment which was conducted in the Botanical Garden of Biology Department in the Faculty of Education /Ibn - Al - Haytham / University of Baghdad, during the spring season of 2014 was the influence of different concentrations of Sodium chloride (50, 100) mM.L - 1 in addition to the control treatment and and Four concentrations of kinetin (25, 50, 75, 100) ppm in addition to the control treatment.It is also aiming to study the effect of application and non application the level 160 Kg.H - 1of NPKZn fertilizer, and their interactions in some morphological characteristics root length, root size, plant height, the internode length, stem diameter, Leaf area, dry weight for root and shoot and the absolute growth rate for shoot.and some physiological characteristics Elements content and protein ratio in root, Elements content, protein ratio, total chlorophyll content, carbohydrates percentage and cytokinine concentration in shoot. Biochemical characteristics (enzyme activity of the enzymes) (SOD, POD and CAT) and some antioxidants that nonenzymatic : (the proline and vitamin C concentration) in shoot. Malondialdehyde (MDA) content in shoot, and some Floral morphological, physiological and biochemical characteristics of fruits (Number of branches, The total number of flowers, Number of blooming flowers, ratio of aborted Flowers, Number of knotted flowers, the number and size of fruits, their wet weight , nitrogen and calcium concentration, protein percentage and vitamin C concentration in fruits of pepper plant California weleander variety.The experiment was designed according to Randomized Completely Block Design (RCBD)with three facters kinetin, manure addition and Sodium chloride,) consisting 90 pots. Seedling of pepper plant was planted on 4/2/2014 acompanied by making all agricultural operations of irrigation and the removal of the jungles. results were obtained can be summarized as follows : 1 - The results showed that increasing the concentration of sodium chloride from zero to 100 mM.L - 1 Led to a decline in morphological, physiological and floral characteristics, with and significant increases in the content average of sodium, chloride, The proportion of flowers aborted and biochemical characteristics. The rate quality effectiveness for enzyme SOD increased from 7.24 to 34.66, POD from 8.10 to 26.35 and CAT from 4.91to 39.57 (unite mg protein - 1) , and proline acid content from 23.05 to 40.30 (mg.L - 1) and vitamin C concentration from 2.67 to 3.68 % and Malondialdihyde (MDA) from 1.84 to 3.86 (µM.gm F.W. - 1)2 - As concerns kinetine treatment effect, the results showed a significant increase in the average of morphological, physiological and floral characteristics, and significant decreases in the average of sodium, chloride contents and biochemical characteristics, in the cased increasing in the concentration of kinetine from 0 to 100 ppm caused decreases in the average of quality effectiveness for enzyme SOD from 25.79 to 21.91, POD from 21.83 to 17.20 and CAT from 27.88 to 21.73 (unit mg protein - 1) , and proline acid content from 34.27 to 31.68 (mg.L - 1), Malondialdihyde (MDA) from 3.15 to 2.85 (µM.gm F.W. - 1) and vitamin C concentration from 3.42 to 3.23 %.3 - application the level 160 Kg.H - 1of NPKZn Manure caused a significant increases in all averages morphological, physiological and floral characteristics, and significant decreases in the average of sodium and chloride contents and biochemical characteristics, fertilizing with the level 160 Kg.H - 1of NPKZn fertilizer caused decreases in the average of quality effectiveness for enzyme SOD from 22.03 to 19.88, POD from 17.70 to 14.53 and CAT from 23.34 to 17.34 (unite mg protein - 1) , and proline acid content from 32.84 to 28.93 (mg.L - 1), Malondialdihyde (MDA) from 2.97 to 2. 75 (µM.gm F.W. - 1) and vitamin C concentration from 3.29 to 3.03 %.4 - Results of the bilateral interpenetration between the Fertilization by fertilizer NPKZn and Sodium chloride concentrations, showed that the fertilizations has a positive and effective influence in reducing the negative effects of sodium chloride in all characteristics studied. 5 - The results showed the positive role of kinetine in decreasing the negative effects of sodium chloride Through bilateral overlap between factors. Especially when the concentration of 75 ppm of kinetine and concentration 100 of sodium chloride In all morphological, physiological and biochemical floral characteristics of plant.6 - The results indicated that the Interference between the fertilizer NPKZn and kinetine has a positive and clear role to both factors increase morphological, physiological, floral characteristics and reduce biochemical characteristics.7 - The effect of the triple overlap between study factors was significant, The results showed That plants sprayed kinetine And fertilization led to a significant reduction of most of the negative effects resulting from the of high concentrations of Sodium chloride in all morphological, physiological and flowering traits. Especially in the concentrations 75 ppm kinetine and in the existence of fertilization.

استخدام الزراعة النسيجية والفطر Glomusmossaes في الازالة الحيوية لعنصري الرصاص والكادميوم وتحمل الملوحة لنبات السيسبان Sesbaniarostrata L == Application of Plant Tissue Culture And Glomusmossaes In Bioremediation of Lead, Cadimum And Salinity Using Sesbaniarostrata L. Plant

اسم المؤلف: التفات فاضل شحاذة الطائي
اسم المشرف: علي هاشم الموسوي | كاظم محمد ابراهيم
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت تجارب عدة لغرض دراسة تحمل نبات السيسبان Sesbania rostrata L. للكادميوم والرصاص وكلوريد الصوديوم على مستوى النبات الكامل ومزارعة النسيجية. استحث الكالس واديم على وسط موراشيج وسكوج 1962 والمجهز 0.3 ملغم/لتر من الكاينتين و2 ملغم/لتر من حامض 2, 4 داي مث | Several experiments were carried out to study cadmium, lead and sodium chloride tolerance at the tissue culture or whole plant levels of Sesbania rostrata. Callus was induced and maintained on Murashige and Skooge (1962) medium (MS) supplemented with 0.3 mg/l Kinetin and 2 mg/l 2, 4 - dichlorophenoxy acetic acid (2, 4 - D) using cotyledons as the source for callus induction. Different concentrations of cadmium, lead and sodium chloride directly or gradually were added to the culture medium as contaminants. Selected tolerant cell lines were subjected to regeneration. Callus showed better tolerance to gradual exposure than direct addition. The concentrations of Potassium, lead, Cadmium and Sodium recorded to 17, 24, 27, 35 ppm when 1.0, 2.0 mg/L and 0.4% of cadmium, lead and sodium chloride were added respectively. The study included the effect of the cytokinin (benzyl adenine, BA) and the auxin (Naphthalene acetic acid, NAA) on the regeneration of shoots from callus tolerant to pollutants reached 76, 87 and 85% for callus tolerant to Cadmium, Lead and sodium chloride respectively, when the combination of 0.7 mg/l BA and 0.2 mg /l NAA was supplemented to the medium. The effect of indole butyric acid (IBA) on rooting of shoots. The concentration 0.7 mg /l gave highest percentage of rooting amounted to 66, 70 and 85% in a medium supplemented with cadmium, lead and sodium chloride respectively. Acclimatization of plantlets using river sand soil recorded 85% survival. The study was also included effects on plant height and fresh weights, shoot fresh weight was superior at the concentration 1.0 and 2.0 mg/l of cd and pb recording 8.7 and 7.5 g respectively. Treatment with NaCl led to increase the root fresh weight recording 19.1 g. Maximum cd accumulation reached 12.5 ppm in shoots, 10.5 ppm for lead in roots, 39 ppm for NaCl in roots. The experimental work investigated the effect of mycorrhiza (Glomus mosseae) on cd, pb and NaCl removal by Sesbania plant when grown in either soil contaminated with cd, pb and NaCl or non - contaminated soil (control). Inoculation with mycorrhiza led to an increase in seed germination percentage (96%), plant height (28 cm) in non - contaminated soil, while achieved an increase in shoot and root fresh weight recording 10.0 and 6.8 g respectively. Inoculation with mycorrhiza also led to a significant increase in cd and pb accumulation in roots reached 9.0 and 87.4 ppm respectively. Meanwhile, Mycorrhiza caused a significant increase in Na accumulation in root system (42.5 ppm) which already affected K concentration as a result of competition with Na recording 16.5 ppm in shoots. Mycorrhiza inoculation led to a significant increase in number of root nodes in non - contaminated soil recording 86%. Finally, mycorrhiza succeded in infecting Sesbania rostrata roots reached 90% in non - contaminated soil while it was 85% in contaminated soil

تاثير الكلوتاثيون وبيروكسيد الهيدروجين وتداخلهما في بعض الصفات النوعية والكمية لنبات الماش Vigna radiat == Effect of Glutathione And Hydrogen Peroxide And Their Interactions On Some of The Quantity And Quality Characteristic of (Mung Bean) Plant Vigna Radiata L.

اسم المؤلف: ايمان حسين هادي الحياني
اسم المشرف: وفاق امجد القيسي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Two field experiments were conducted during the spring and autum growth season of Vigna radiata L. in the year 2014 at botanical garden of Biology Department, Collage of Education for Pure Science (Ibn AL_Haithem) University of Baghdad.The experiments aimed to study the effect of glutathione and hydrogen peroxide, and their interactions on some of quantity and quality characteristic of plant Vigna radiate L.The treatment of glutathione (0, 25, 50, 75, 100) mg.L - 1 While the H2O2 (0, 5, 10, 15) ml mol. L - 1.The two experiment were designed as Randomized Complete Block Design as factorial experiment with two factors with three replication which included 60 experiment units the area of each one (1×1`) m. Results were analyzed Statistically and compared using average L.S.D at 0.05. The results of the experiments showed that glutathione revealed significantly increased all study parameter espically in 100 mg.L - 1 concentration, stem diameter by 54.56%, 28.04%, leaves number 69.34%, 53.14% for two seasons, fresh weight 44.20% for first season, the dry weight 37.43%, 91.46%, leaf area 61.61%, 151.23%.leaf area index 86.61%, 146.35%, biomass duration 60.48%, 101.06%, absolute growth rate (AGR) 32%, 92.68%, inflorescences number 56.55%, 39.90%, flower`s number 71.97%, 22.60%, root length19.82. %, 26.20%, root dry weight 76.10%, 79.25%, total function of SOD 69.32%, 40.52%, total Function of POD 29.45%, 82.25%, total Function of GPX 30.90 %, 63.62%, chlorophyll concentration of a 73.48%91.40%, chlorophyll concentration of b 35.42%, 17.67% for two seasons respectively. The total chlorophyll content 13.69%, for second season, Caroten concentration 207%, 309%, proline concentration 84.47%, 31.75%, (MDA) concentration 6.25%, 38.35%, glutathione. concentration 41.49%, 23.62 %, H2O2 concentration 52.16%, 33.24%, pods number 17.43%, 16.93%, 100 seeds weight 22.95 % 22.48%, , 93.62%, seeds yield 52.17%, 43.70%, the carbohydrate percentage 64.07%, 19.21 %, the protein percentage 22.32% for first season while the results of effect of soaking seeds with H2O2 specially with 15m ml.L - 1 concentration increased stem diameter 45.52%, 34.00%, leaves number 18.55%, 30.75%, Lateral branch 26.06%, 48.04% for Two seasons respectially, fresh weight 35.45% for first season, dry weight 40.89%, 43.85%, leaf area 53.24%, 64.53%, leaf area index 92.30%, 62.39%, biomass duration 35.35%, 57.53%, abosult growth rate34.78 %, 48%, in, inflorescences number 34.98%, 38.10% flowers number 2.987, 20.13%, root length19.44 %, 19.36%, root dry weight 99%, 96.52% total functhion of (SOD)100%, 2853%, total function of (POD) 176.57%, 40.58%, total function of (CAT)118.29%, 71.78%total functhion of (GPX)12.80%, 61.40%, Caroten concentration 54%for second season, prolineconcentration 40.93%for first season, (MDA) content 17.31% for second season, glutathione concentration, 13.68% 24.29%, H2O2 concentration 26.53%, 30.58%, number of pods 42.77%, 20.51%, seads number /pod 22.93%, 22.93%, 100 seeds Wieght 24.07%, 24.86%, seeds yield 16.69% for first season, the solubly carbohydraty percentage 43.26%for first season, the protein percentage 11.50%, 25.18 % for two seasons respicatlly, while chlorophyll a decreas concentration ed by 15m ml.L1 12.87%for second season, total chlorophyll concentration 39.66%, 26.22 for two season respicatlly. There were high significant interaction between glutathione and H2O2 in all study parameter characters in the two experiments.

تقييم نوعية مياه نهر الفرات (بين مدينتي الكفل والشنافية) وسط العراق == Assessment of Water Quality of Euphrates River (Between The Cities of Al - Kifl And Al - Shinafiyah) Iraq

اسم المؤلف: دنيا باهل جدعان صلال الغانمي
اسم المشرف: حسين يوسف خلف الركابي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: البيئة المائية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية على جزء من بيئة نهر الفرات الممتد بين مدينتي الكفل والشنافية لمدة عام تقويمي من ايار 2013 ولغاية نيسان 2014 واختيرت اربعة مواقع مختلفة وموزعة على منطقة الدراسة شملت ثلاث محافظات هي : بابل - النجف - القادسية (الفرات الاوسط - العراق)، | The current study has applied on the Euphrates River for a full year from May 2013 until April 2014. Four sites selected along the river whithin three provinces; Babylon, Najaf, Al - Qadisiyah. Assessment of water quality done by using Canadian Council of Ministers of Environment (CCME) and Index of Biological Integrity (IBI) for the purpose of determining the validity and quality of its water for the General purpose and for Drinking and Irrigation.The physicochemical properties and biological (Phytoplankton) were studied during the study period. These properties were; temperature of the air and water, light penetration, electrical conductivity, salinity, turbidity, water current speed, total suspended and dissolved solids, dissolved oxygen, biochemical oxygen demand, chemical oxygen demand, carbon dioxide, total alkalinity, acidity, total hardness and ions of calcium, magnesium, sodium, potassium, and the sodium adsorption ratio, chlorides, sulfates, boron, nitrite, nitrate, phosphate, silica and some heavy metals such as cadmium, lead, zinc, copper, chromium, manganese in both phases : dissolved and particulate. results showed that the values of the physical and chemical factors ranged as follows : (7 - 47) C, (10 - 31) C, (14 - 177.5) cm, (1020 - 5600) µs/cm, (0.65 - 3.46) 0%, (0.07 - 129) NTU, (27.03 - 115.57) cm/s, (591 - 2985) mg/L and (0.001 - 0.128) mg/L, (7.04 - 8.8), (6 - 11.8) mg/L, (0.13 - 6.6) mg/L, (8.2 - 53) mg/L, (81.4 - 198) mg/L, (30 - 200) mg/L, (6 - 46) mg/L, (490 - 929.67) mg/L, (83.37 - 290.18) mg/L, (0.79 - 174.84) mg/L, (92.5 - 713) mg/L, (1.9 - 11.18) meq/L, (6 - 133) mg/L, (97.97 - 861.73) mg/L, (329 - 1040) mg/L (0.01 - 4.23) mg/L, (0.008 - 1.33) µg/l, (2.16 - 44.74) µg/L, (0.012 - 0.59) µg/L and (104 - 514) µg/L respectively.Concerning the heavy elements, the values of dissolved cadmium ranged between (undetectable - 0.075 mg/L) and it's particulate between (0.0043 - 0.2705) mg /L and dissolved lead between (undetectable - 0.0232 mg /L) and it's particulate between (0.0045 - 0.148) mg/L and dissolved zinc between (un - detectable - 0.0168 mg/L) and it's particulate between (0.0115 - 0.316 mg/L) and dissolved copper (undetectable - 0.0273 mg/L) and copper is particulate between (0.0062 - 0.063 mg /L) and dissolved chromium between (undetectable - 0.0193 mg/L) and the chromium is particulate between (0.0043 - 0.0449) mg/L and dissolved manganese (undetectable - 0.0256) mg/L and it's particulate between (0.0085 - 0.0739) mg/L.The biological tests include Qualitative and Quantitative Study on phytoplankton and also included calculating the total number of coliform way casting dishes by using the MacConkey Agar media.The number of diagnosed species of Phytoplankton was 295 species, belonging to 74 genera. The Diatoms Bacillariophyceae won the biggest part of it as it formed 160 species and 35 genera any rate (54.24%), followed by green algae (70 species belonging to 24 genera) and green algae (52 species belonging to 11 genera) then Euglenophyceae (10 species belonging to both sexes), while golden brown algae and algae Pyrrophyceae were less algal groups registered as belonging to the two single - sex. Some types of phytoplankton existed in most of the duration of the study, including Bacillaria paxillifera, Cocconies placentula, Cyclotella meneghiniana, Diatoma elongatum, Fragilaria virescence, Melosira ambigua, Synedra acus.The total number of phytoplankton is estimated between (104.9 - 4460.09) cell × 103/liter. An increase in the number of phytoplankton in July and January was observed clearly. The concentrations of chlorophyll - a were ranged between (0.0693 - 9.78 mg /L). While the total number of results for coliform were (5 - 281 × 103) cells/liter, The water quality of the evidence Account (Canadian model) using a number of physical, chemical and Biological properties. The results showed that the waters of the Euphrates are located within categories (Poor - Good) with respect to the public evidence of the quality of water of this river while it was located within categories (Poor - Acceptable) with respect to their use for drinking, While its use for irrigation purposes, it was clear that run river water data fall into two categories (Poor - Good). The results of the analysis of the main components Principal component Analysis (PCA) indicate that the nitrates and the total number of coliform and dissolved oxygen, phosphate, zinc, lead and Biological oxygen demand and pH are the biggest impact on the water quality of the index values of the variables of general purpose.These factors are responsible for the deviation index values, followed by turbidity and total hardness and electrical conductivity and temperature of the water, copper, chromium, and cadmium heat. But with the biggest impact of the index water quality factors for drinking purposes are chloride and electrical conductivity and total hardness, magnesium, turbidity and basal college, sodium, calcium, copper, sulfates, nitrates and coliform, zinc and dissolved oxygen, pH, manganese and lead followed by boron nitrite, cadmium, chromium and vital requirement for oxygen. While the chloride and conductivity electrical, the proportion of sodium, copper, zinc and adsorption of boron have greatest influence on the value of water quality index for irrigation, followed by manganese, cadmium, chromium, lead and pH.The phytoplankton index of Biotic Integrity (P - IBI) has been calculated using ten units including (relative abundance of green algae, green algae, pyrrophyta, Euglenophyta, Xanthophyta, Centralales, pennales, species richness index, the density of phytoplankton and chlorophyll a) The results showed that phytoplankton index of Biotic Integrity values ranging between (47 - 74) in the first position and (47 - 67) in the second location, and (45 - 76) in the third site and (45 - 71) in the fourth site. In other words, phytoplankton index of Biotic Integrity with estimating index (good - poor). The results showed the effectiveness of phytoplankton index of Biotic Integrity values in estimating the environmental changes.

دور اجهاد المضاد الحياتي (سيفتازديم) في امراضية الممرضة البولية الايشريكية القولونية المنتجة للبيتا لاكتاميز موسعة الطيف في الفئران المختبرية == Role of Antibiotic (Ceftazidim) Stress On Pathogenicity of Uropathogenic Extended Spectrum ? - Lactamaes E. Coli In Laboratory Mice

اسم المؤلف: علي حسون حمادي عبد الله
اسم المشرف: محمد قيس العاني | حارث جبار فهد
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: الانبار
الصفحات الاولى:
المستخلص: جمع 60 نموذجا من عينات بول وسط المجرى من المرضى الراقدين في المستشفى الذين يعانون من اخماج المجاري البولية من مستشفى اليرموك ومستشفى الكرخ ومستشفى القديس رافائيل/ الراهبات للمدة من 27 شباط 2014 ولغاية 25 اذار 2014، وقد اظهرت نتائج الزرع البكتريولوجي الحصو | Sixty mid - stream urine specimens were collected from patients presented with urinary tract infections whom attending Al - Numan, Al - Yarmouk, Al - Karkh, and Saint Raphael/Al Rahibbat hospitals in Baghdad for the period February, 27th to March, 25th 2014. Bacteriological culture identified fifty (80.33%) isolates as E. coli depending on cultural and biochemical characteristics as well as Api and Vitek 2 automated systems. However, 82% of isolates developed ? - hemolysis on blood agar plates.Susceptibility of all isolates against seven commonly prescribed antibiotics were investigated and 96, 82, 84, 92, 92, and 56%. of isolates were resistant to ampicillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, cefalothin, imipenem, and meropenem, ceftazidime, respectively.Concomitantly, ? - lactamase production was detected phenotypically by disk replacement method. The results showed that 82% of all isolates have the ability to produce ? - lactamase. Interestingly, correlation coefficient between ? - lactamase and hemolysin production was 0.99.Using microtiter plate method, all E. coli isolates were tested for their ability to form biofilm. The results revealed that 36, 54, and 10% of isolates formed biofilm with variable thickness; weak, moderate, and strong, respectively.Alongside with previous findings, blaCTX - M - 2, blaVIM, blaTEM, and blaPER were identified in 18, 12, 10, and 4, respectively. Nevertheless, none of blaOXA - 1, blaOXA - 4, blaOXA - 30, blaIMP, blaCTX - M - 1, blaCTX - M - 9, blaKPC, and blaSHV. The isolate number 11 harboured the highest number of genes (blaTEM, blaVIM, and blaPER) among all tested isolates. Twelve albino mice was divided randomly into four groups comprising A through D injected with ceftazidime at sub MIC, E. coli 11, E. coli 11 with ceftazidime solution, and standard strain, respectively.Histopathological sections did not show any changes in respeuct to group A. however, group C suffered signs of infection less than those appeared in group B sections. Simultaneously, group D suffered intense histpathological changes more than other groups infected with resistant isolate.

دراسة علاقة سم Ochra. A بحالات الفشل الكلوي غير معروف الاسباب في محافظة القادسية == Study The Relationship of Ochra.A With Kidney Failure of Unknown Case In Al - Qadisiya Governorate

اسم المؤلف: هدى رحيم هاشم الموسوي
اسم المشرف: بهيجة عبيس حمود الخالدي
الموضوع العام: علوم الحياة
السنة: 2015
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة لتحديد العلاقة بين السموم الفطرية وحالات الفشل الكلوي غير معروف الاسباب لدى المرضى المراجعون الى مستشفى الديوانية التعليمي للفترة من اذار/2012 الى كانون الثاني/2013 والذين تم تشخيصهم من قبل الاطباء المختصون. اظهرت نتائج فحص عينات الادرا | This study was designed to detection the Relationship between some fungi and it’s toxin with Renal falier in patients suffer from Renal falier attended to Al - Diwanyia teaching hospital in period from March/ 2012 to January/ 2013, These patients were diagnosed by physician in above hospital. By using Thin Layer Chromatography (TLC) the results showed 23.07% from patients blood specimens had Ochratoxin A, 19.23% from patients urine specimens had Ochratoxin A 38.46% from patients had Ochratoxin A in blood and urine where only 59.61% your blood and urine were clear from above toxin, So healthy persons were had Ochratoxin A in your blood and urine by rate 20% 40% respectively and 10% from them had Ochratoxin A in your blood and urine and only 65% from them don’t had Ochratoxin A in their blood and urine.the concentration of Ochratoxin A in blood were ranged between 6.2 - 25.8 ?g/kg from weight boody and 4 - 10.3?g/kg in urine and in healthy reach concentration of Ochratoxin A in blood 2.3 - 3 ?g/kg from weight boody and 4.3 - 4.6 ?g/kg in urine.The data of this study showed the male highly infected 87.5% with ocratoxin while in female 70% and 71 - 80 years age had highly infected 92.85% with ochratoxin A. The results of study of biochemical parameter in patients showed highly raised in WBC and urea and Keratinin to reach to 12000 corpuscle /ml3 310 mg/dl, 12.2 mg /dl respectively and lowed the activity of alkaline phosphates enzyme and albumin into 2.2 u/l, 1.5 gm/dl, respectively and suger 120 mg /dl. The results of isolation and diagnosis by depending on Morphological, Microscopical and Biochemical characters showed there were two species of fungi belong to Aspergillus sp, include A. ochraceaus 25.42%, A. niger 12.71% and A. candidus 15.25% and Candida albicans 46.61% were isolated from urine patients where only C. albicans and C. krusi were isolated from blood of patient by percentage 66.66% and 33.33% respectively. So the results of single PCR method selected to detection the OCR and PSK genes in Aspergills spp which responsible for ochratoxin A production , showed 83.33% from A. ochraceaus isolates had OCR gene (907 bp) and 66.66% from A. nger had PSK gene (584bp)Also the results of this study showed A. ochraceus and A. niger had ability to produce metabolic compounds when grow on yeast - extract broth and these compounds had significant toxic effects(P?0.05) in biochemical parameters in albino rats represented raised number of white blood cells (WBCs) into 10610 cell/µm3, urea into 228 mg/dl and Keratinin into 15 mg/dl in blood of animals which treated with 5000 ?l/kg from metabolic products of above fungi respectively, comparative with control groups 10100 cell/µm3. in other side, these metabolic products caused in same dose lead to lower of albumin and activity of alkaline phosphatase enzyme into 2.8 mg/dl and 3.7 u/l respectively where normal level for this parameter in control groups.Histological study showed the dose of metabolic products 500 - 5000 µl/kg for both fungi caused pathogenic changes in kidney represented by, atrophy highly proliferation of cells in endothelial layer of Bowman's capsules in addition to hemorrhage and Focal agrgregate inflammatory cells.In other side, study of toxicity of ochratoxin A which extracted by using thin layer chromatography (TLC) teqnique from urine and blood of patients and yeast extract broth media that had growth of A. ochraceus and A. niger showed signification effects(P?0.05) for this compound in physiological and biochemical parameters also caused significant effects in histological parameters similar to these effects which occurred in previsious experiments while these effect don’t appear in control groups.

الكشف عن الجينات المقاومة للكاربابنيم لبكتريا Acinetobacter baumannii المعزولة من عينات سريرية في محافظة بابل == Detection of Carbapenem - Resistant Genes of Acinetobacter Baumannii Isolated From Clinical Samples In Babylon Province

اسم المؤلف: رعـد عبد العباس حمزة الهرموش
اسم المشرف: ايمان محمد جار الله
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: للفترة من اذار 2014 الى اذار 2015 تم جمع 1300 عينة سريرية مختلفة (عينات ادرار ومسحات جروح وحروق وعينات دم ومسحات من الفم والاذن والعيون بالاضافة لعينات البراز والقشع) من اثنين من مستشفيات محافظة بابل (مستشفى الحلة التعليمي ومستشفى بابل التعليمي للنسائية و| During the period between March 2014 and March 2015, a total of 1300 clinical specimens (urine, wounds, burns, blood, throat, ear, eye, stool, and sputum) were collected from two hospitals in Babylon province; Al - Hillah Teaching Hospital, and Babylon Hospital for Maternity and Pediatric. All specimens were cultured and 10 Acinetobacter baumannii isolates were obtained from clinical specimens in a percentage of (0.76%) distributed in blood 1 (0.6%), urine 2 (1%), wound infection 1 (0.75%), burn 5 (0.85%) and throat 1 (2%). This study was conducted to determine the occurrence of carbapenem resistant A. baumannii isolates obtained from Hillah hospitals. Isolates were identified according to API20E system and further confirmed using PCR technique. Antibiotics susceptibility was assayed by using disks diffusion method according to CLSI recommendations. All isolates revealed positive results with rapid iodometric test indicting the ability of beta lactamase production. Phenotypic detection of carbapenemase production was performed using the imipenem - EDTA disk and modified Hodeg's test (MHT). Then isolates were subjected to monoplex PCR targeting blaOXA - 51, blaOXA - 23, blaOXA - 24, blaOXA - 58, blaIMP, blaSIM blaNDM - 1, blaNDM - 2 and blaVIM genes, as well as, efflux pumps genes. One of the identified isolates (10%) was found to be imipenem, meropenem and ertapenem resistant, this isolate gave positive result with the imipenem - EDTA disk and (60%) of isolates were positive to MHT. PCR experiments showed ; all isolates were harbored blaOXA - 51 gene, four (40%) isolates were harbored blaOXA - 23 gene, three (30%) isolates were harbored blaOXA - 24 gene, four (40%) isolates were harbored blaOXA - 58 gene, five (50%) isolates were harbored blaIMP gene, six (60%) isolates were harbored blaSIM gene, two (20%) isolates were harbored blaNDM - 1 gene, four (40%) isolates were harbored blaNDM - 2 gene, while none of these isolates harbored blaVIM gene. The present findings suggest that emergence of (OXA - 23, OXA - 24, OXA - 58, IMP, SIM, NDM - 1, NDM - 2) carbapenemase producing A. baumannii clinical isolates in Hillah City hospitals. Also there are indicative appearance of efflux pump genes ; (50%) for Ade - A and Ade - B each other, (40%) for Ade - C, (80%) for all of Ade - R and Ade - S, (100%) for all of Ade - I and Ade - J, and (50%) for Ade - Y. All isolates of A. baumannii appear as MDR, while only one appear to be as PDR. The trans - mobility of resistant genes was examined by trans - conjugation experiment ; the results indicated that only blaIMP and blaOXA - 23 genes were transferred horizontally in the current study. In attempting to investigate any blaNDM gene variation the results showed some different than NCBI - Blast A. baumannii blaNDM - 1 and blaNDM - 2 it may as a unique genotyping.These results revealed that carbapenemase producing A. baumannii were detected in both phenotypic and genotypic methods (PCR). This underlies the importance of their accurate identifications and reporting to prevent the emergence of complete resistance to the most potent drugs against A. baumannii in Babylon province.

النمو، الفعالية الانزيمية والتعبير الجيني للانزيمات المحللة للبروتين (Protease) في الفطر الجلدي Trichophyton rubrum == Growth, Enzyme Activity And Gene Expression of Proteases In A Dermatophyte Trichophyton Rubrum

اسم المؤلف: سارة كريم كاظم الزبيدي
اسم المشرف: جواد كاظم الجنابي | عدنان حمد الحمداني
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: صممت الدراسة الحالية لتوصيف الانماط الجزيئية والمظهرية للنمو والفعالية الانزيمية للانزيم المحلل للبروتين والتنوع الوراثي وعلاقته بالتعبير الجيني بين عزلات الفطر الجلدي.Trichophyto rubrum ولهذا الغرض جمعت 150 عينة سريرية (قشطات جلدية (80), اجزاء شعر (60) | The present study was designed to characterize the morphological and the molecular patterns of growth, enzymic activity of proteases, genetic diversity and their correlation with gene expression among strains of Trichophyton rubrum. For these purpose, A total of 150 clinical specimens (skin scrapings (80), hair fragments (60) and nail clippings (10)) were collected from 100 patients (70 males&80 females) whom clinically diagnosed with dermatophytosis after attendingto the dermatology and venereal diseases centre of Mergan Teaching hospital and private clinic in Babylon city from Feb. 2014 to May 2014. T. rubrum were isolated and identified using cultural, biochemical and physiological tests. Isolates were then subjected for confirmation by PCR, genotyping using RFLP - PCR, sequencing and registration of these sequences in GeneBank for obtaining accession numbers then phylogeny. The growth conditions, were tested, in addition to, the genetic expression of proteases (exocellular protease and endocellular aminopeptidase) were determined using Real - Time PCR. Out of 150 specimens, 24 (16%) isolates were dermatophytic fungi and only 5 (20.8%) of them belong to T. rubrum. The influence of cultural conditions in growth of T. rubrum showed that the 30 ?C, pH 6, SDA medium and 7 days incubation were the optimal conditions for its growth. Proteolytic activity of isolates of T. rubrum against casein as a substrate revealed an ability to produce protease in solid and liquid media after 14 days of incubation. This activity was varies according to the type of isolate where the isolate No.1 (isolated from skin) gave a high proteolytic activity (5.6cm) in solid media and (80.1U/ml) in liquid media in comparison with other tested isolates (No.2 - No.8). on the other hands, the 9 days of incubation, 30 ?C, pH 7 and 0.5% substrate concentration were the optimal condition for proteolytic activity of these isolates. The molecular tests confirmed that all tested isolates belong to T. rubrum with amplicon size (601bp) after amplification of ITS1 primers using PCR technique. While the RFLP - PCR technique showed the presence of two genotypes (I%II) belong to T. rubrum with subgenotypes (Ia - Id) and (IIa - IId) respectively. The relative quantification of proteolytic activity (exocellular protease and endocellular aminopeptidase) produced by T. rubrum genotypes were expressed by using Real - Time PCR after amplification of the target gene of ptotease and aminopeptidase in comparison with housekeeping gene (? - actin) as a reference gene. The results showed the up - regulation of gene encoded to exocellular protease than the down - regulation of endocellular aminopeptidase produced by T. rubrum in the presence of casein as a substrate. The internal transcribed spacer 1, partial sequence, 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence and 28S ribosomal RNA gene, partial sequence of local isolates of T. rubrum were used for sequencing, registration in Genebank - NCBI and phylogeny. Five accession numbers were recorded and available to NCBI, EMBL in Europe and the DNA Bank of Japan. These accession numbers were : KP979787, KP979788, KP979789, KP979790, KP979791. Phylogenetic relation between local strains and world strain showed a high identicasl with T. rubrum (GQ376105.1). This is the first study in Iraq which employed sequencing, registration of sequences in Genebank - NCBI and carrying out phylogeny of local and world strains of T. rubrum.

التشخيص الجزيئي للفطر المحاري Pleuroutus ostreatus وفاعلية بعض المخلفات الصناعية في انتاجه وقابليته الخزنية == Molecular Diagnosis of The Oyster Mushroom Pleuroutus Ostreatus And Effectiveness of Some Industrial Waste In Its Production And Its Ability Storage

اسم المؤلف: سولاف حامد تيموز
اسم المشرف: عبد الامير سمير سعدون
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تضمنت هذه الدراسة انتاج الفطر المحاري ostreatus Pleuroutus باستعمال اوساط زرعية محضرة من مخلفات صناعة محلية وما لها من اهميه من الناحية الاقتصادية كونه يعد عنصرا غذائيا مهما يحل محل البروتين الحيواني المتمثل باللحوم، وما لهذا الفطر من اهمية طبية وعلاجية ا | This study included the production of mushroom oyster Pleuroutus ostreatus by using agricultural media prepared from local industry and which has importance in the economic district it's consider important nutrition element instead of animal protein representive by meat. this fungus has importance of medical and therapeutic, the results showed existence of chemical substances concentrated in the fruiting bodies such as Galic acid and Statin and other substances. that have had an active role in reducing cholesterol in the blood as well as break the clot blood through the presence of certain types of enzymes that break down blood clotting, which may occur, the results showed when studying the mushrooms from the genetic diagnosis molecularly using the technique of the PCR to determine the type of isolation that gives the production of economically significant. Results showed the presence of gene hereditary called Osteareolysin who works with several aspects of the mushrooms which give the recipe contrast pathogenic fungi plant also this study included re - using some of the remnants of plant starch corn husks and Al - gluten and corn embryos. the remnants of Maysan sugar Factory of material Molasses and Molass Baggaz and the study of their role as carrying materials, central ferment and growth of the oyster mushroom during agricultural it on these remnants. The results of the chemical analysis of the remnants of the sugar indestrey in Maysan Molasses and Baggaz and waste plant starch in Al - hashemeyeh / Babylon Al - Baggaz and yellow corn straw showed that molasses substance was contain the amount of sugars which sometimes reach to 41%, that making it as a good base of food to mushroom. When added molasses at different levels 2, 4, 6% to the central media the study showed a positive relationship between the levels of molasses and the radial growth of the oyster mushroom. The concentrations 25, 50, 100% of the leaky innate showed ability Tdhadah high - fungal plant pathogen (Aspergillus niger, Aspergillus flavus, Fusarium solani, Stymphllium sp., Penicillium sp.) Antigonsim when studying the negative ability of the fungus. The results showed a significant increasing in the growth standards and gaining characteristics of the oyster mushroom in the mass production of fruiting bodies called the gaining total according to the wet weight and it was higher production on corn husks and pomace dates 761.89, 735.13 g /kg of media and concentration of molasses 6%. when measuring efficiency of the fungus to product the largest amount of fruiting bodies observed that the middle pea - corn husks and pomace dates gave a larger amount of the remaining media and a concentration of 6% 76.18, 73.51%, have been affected by the weights of pods fungal change is another factor which affected by rates of molasses. The results were closely between nutrition media but nevertheless observed superiority of Corn husks and Pomace dates on the rest of media (15.04, 13.77)by using 6% concentration of molasses. when calculate the lengths of the necks of the body fruiting because of the great importance in a statement recipes - gaining produced and influenced by user medai - aligned and concentrations of molasses, as there were differences moral at all concentrations of molasses, when calculating the percentage of dry matter increased significantly increase the concentration of molasses and all concentrations compared with a comparison of strew wheat, which amounted to 12.16% when adding 2% molasses observed the emergence of highly significant differences between treatments and compared with control treatment, which gave treatment of Pomace dates highest rates, followed by corn husks and corn embryo and Al - gluten and Baggaz 8.21, 9.00, 9.28, 9.29%, respectively. For the purpose of obtaining material efficient in maintaining the objects fruiting intact throughout the storage period tested preservatives (such as Sorbic acid and black pepper) and concentrations 40.20g / l of articles in a row leads to prolong longevity reservoir simulation of the objects fruiting and maintain the quality characteristics of the objects fruiting. The results showed that the addition of material to the feeder molass medai AGRO of oyster mushroom had been increased the proportion of protein in the fruiting bodies, either with respect to calculate the proportion of carbohydrates had been found that the percentage of carbohydrates are associated with a high positive correlation relationship between the concentration of molasses and the different transactions that were used in the experiment. The oils were represented less than what can be had reached attribute to 0%. Well conducted safety checks the health of the mushroom oyster and the results were that the fungus free of toxins by anatomy organic to members liver, kidney and intestines and by the standards of blood physiological mice tested. And the importance of this project calculated the economic viability and profits and revenue obtained from such a project.

الكشف عن بكتريا الكوكسيلا بيرنتي في حالات الاجهاض في الانسان والمجترات الصغيرة في محافظة ذي قار == Detection The Role of Coxiella Burnetii In Abortion of Human And Small Ruminants In The Thi - Qar Province

اسم المؤلف: عباس دخيل مطر جبر الجوراني
اسم المشرف: عبد الله كاظم هندي | محمد عبد الله جبر
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: الحمى المجهولة من الامراض الواسعة الانتشار في العالم تحدث بسبب جرثومة الكوكسيلا بيرنتي.هذه الجرثومة تسبب عدة امراض الاجهاض هو الاكثر حدوثا. المخاطر البيولوجية وانشار هذه البكتريا على النساء المجهضة والحيوانات في العراق غير معروفة. لذلك هدفت هذه الدراسة ال | Query fever is a worldwide distributed disease caused by Coxiella burnetii bacteria causes several disease main of this disease is abortion, the biological hazard and prevalence of this bacterium on the aborted woman and small ruminants are not known in Iraq. Therefore this study was aimed to detection and isolation of Coxiella burnetii as a causative agent of abortion in woman and female of small ruminants.352 samples were collected includes human samples and animals samples. A total of 120 blood aborted women, 7 breast milk samples, 20 placental samples and 50 blood samples from normal women as control groups, these samples collected from Bent Al - Huda hospital in Thi - Qar province. Animals samples, a total of 80 aborted animal blood samples, 15 milk samples, 10 placental samples and 50 blood samples, these samples from normal animals as controls group, these samples collected from veterinary hospital in Thi - Qar province. The results of this study showed a high incidence of abortion occurs in first trimester of gestation (41.666%) and high incidence in rural regions (64.16%) and also age group from 21 to 30 years old (50%).The methods used for detection of Coxiella burnetii includes serology tests, polymerase chain reaction (PCR) technique for confirmation serological test and isolation on embryonated chicken eggs and detection by PCR technique. Out of the 120 women serum samples analyzed by enzyme ELISA, the results of percentage of anti - Coxiella burnetii IgM and IgG in human samples were 36 (30 %). The percentage of anti - C. coxiella IgM in human samples were 10 (8.333%), while the percentage of anti - C. Coxiella burnetii IgG in human 26(%21.667%).. In animals total of (80) serum samples, the percentage of anti - C. coxiella IgM and IgG in animals' serum samples were 31(38.75%) The percentage of anti - Coxiella burnetii IgM in animals 10 (12.5%), while, the percentage of anti - C. coxiella IgG in animals are 21(26.25 %). PCR technique used for identification of Coxiella burnetii in human and animals samples by targeting three genes including outer membrane protein (com1 and com2), 16S rRNA and transposase insertion element (IS1111) genes. In human blood samples the com1 and com2 genes detected in 23of 120 (19.166%) samples and in breast milk samples 1 of 7 (14.28%). and not detected in placental samples. The 16S rRNA gene was detected in 16 of 120 blood samples (13.33%) and in breast milk samples and placental samples were not detected. The IS1111 gene was detected in 9 of 120 blood samples (7.5%) in human blood samples, also were not detected in milk and placental samples.. In animals blood samples the com1 and com2 genes were detected in 19 of 80 blood samples (23.75%) and were not detected in milk and placental samples. The 16S rRNA gene detected in the same percentage of com1 and com2 genes 19 of 80(23.75%) and in the milk samples and placental samples not detected. The IS1111 gene detected in 10 of 80 (12.5%) in animal blood samples, also were not detected in placental samples. An isolation of Coxiella burnetii which identified by ELISA and PCR via inoculated Coxiella burnetii buffy coat samples in embryonated chicken eggs from 6 to7 days old and then completed an incubated to 10 - 15 days, harvested yolk sac contains then detecting Coxiella burnetii by PCR. The results of PCR after inoculation of (43) samples were positive in PCR (100%). Statistical analysis revealed no significant different between ELISA test, PCR and isolation results in human and animals samples. These mean that we can use the ELISA in the primary diagnosis. The correlation coefficient was highly significantly between human and animal samples at 0.01%.

دراسة مناعية ووراثية تتبعية لمرضى العقم من الرجال == A Prospective Immunological And Genetic Study of Infertile Men

اسم المؤلف: علي عبد الزهرة مهدي الفحام
اسم المشرف: يحيى كاظم السلطاني | عبد الزهرة كاظم محمد علي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الفسلجة
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: ان من الحقائق العلمية الثابتة اليوم ان الفحص الروتيني للسائل المنوي يعد عاملا تنبؤيا اجماليا للقدرة الاخصابية للرجال، ولذلك فان حاجة متزايدة بدات تبرز لاكتشاف فحوصات وظيفية جديدة في عملية تقييم العقم عند الرجال. ان الهدف الاساس من هذه الدراسة هو تعزيز الج | It is a scientific fact today that routine seminal fluid analysis is a key predictor of male reproductive potentiality ; so that there is an increasing need for finding out other functional tests in the assessment of male infertility. The main goal of the current study is to consolidate the scientific and practical efforts concerned with male infertility assessment especially from immunological and genetic aspects. The study was carried out between January 2013 and December 2013 including one hundred (100) selected infertile men who attended the Fertility Center in al - Sadr Medical City in Al - Najaf Al - Ashraf Governorate, the study also included twenty (20) healthy volunteer fertile men as a control. Serum and seminal antisperm antibodies (ASA) were determined by ELISA (Enzyme Linked ImmunoSorbent Assay) technique ; sperm chromatin condensation was evaluated by aniline blue staining (AB) ; sperm DNA fragmentation (damage) was evaluated by toluidine blue staining (TB). The results showed that the incidence and concentration of serum and seminal plasma ASA in the infertile men were significantly (p<0.05) higher than that in control fertile men. The incidence and concentration of serum and seminal ASA were also significantly (p<0.05) higher in normozoospermic infertile patients than that in control fertile men. There was a high significant negative correlation (p<0.01) between the concentration of serum and seminal plasma ASA in the infertile men and sperm motility and progressive motility, the concentration of serum and seminal ASA also showed a high significant positive correlation (p<0.01) with sperm agglutination, and a significant positive correlation (p<0.05) with seminal WBC count. The results also revealed that there were no significant effects (p>0.05) for patients' age, infertility duration, ABO blood groups and smoking habits on the levels of ASA in the serum and seminal fluid, while higher significant increase (p<0.01) in serum and seminal ASA concentrations was observed in infertile patients with varicocele as compared with those without. The results have also revealed that the percentage of sperm with chromatin decondensation and DNA damage has shown a high significant (p<0.01) increase in infertile patients compared to fertile men. There was also a significant increase (p<0.05) in sperm DNA damage in normozoospermic infertile patients comparing with fertile men, but no significant difference (p>0.05) was found in sperm chromatin condensation between normozoospermic patients and fertile men. Both sperm chromatin decondensation and sperm DNA damage showed a significant positive correlation (p<0.05) with sperm morphology, and a significant negative correlation (p<0.05) with sperm concentration, motility and progressive motility. The present study also revealed that the level of chromatin decondensation has been affected by patients' age, infertility duration, smoking habits and varicocele. It was observed that the higher percentage of chromatin decondensation was recorded in patients older than 37 years, and in patients subgroups with infertility duration more than 15 years, it was also higher in smoker as compared with non - smoker patients and in those with varicocele as compared with those without. Similarly, the level of sperm DNA damage has also been affected by patients' age, smoking habits and varicocele. It was observed that the highest percentage of sperm DNA damage was recorded in patients older than 37 years. The highest level of sperm DNA damage was also seen in smoker patients as compared with non - smokers and in those with varicocele as compared with those without. In contrast, the effect of infertility duration on sperm DNA damage was not statistically different (p>0.05). The correlation and regression results recorded high significant correlations (p<0.01) between serum ASA and seminal plasma ASA, and between sperm chromatin decondensation and sperm DNA damage. However, there was no significant correlation (p>0.05) between the level of serum and seminal ASA and each of sperm chromatin decondensation and sperm DNA damage. The current study concluded that the defects in sperm chromatin status (chromatin decondensation and DNA damage), and the immunological disorders caused by serum and seminal plasma ASA may - at least partially - contribute to the etiology of infertility of the patients under study, even in those with normal seminal parameters. However, it seems that ASA affect fertility in a pathway that is different from that affected by sperm chromatin defect. It was recommended that both sperm chromatin staining techniques and ASA tests could be routinely used as complementary tests to diagnose infertility.

التفكك الحياتي لمبيدي الملاثيون والدورسبان باستخدام مزارع احاديه وخليطه من البكتيريا == Biodegradation of Malathion And Dursban By Mono And Mixed Bacterial Cultures

اسم المؤلف: فائزة كاظم عمران
اسم المشرف: عادل مشعان ربيع
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: البيئة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: عزلت 45 عزلة بكتيرية محليه من مجموع 30 نموذج تربه ملوثة بالمبيدات والتي جمعت خلال فترات زمنيه مختلفة ومن مناطق مختلفه في بغداد. اختبرت قابلية العزلات البكتيريه على تفكيك مبيدي الملاثيون والدورسبان. اجريت غربلة اولية وثانوية باستخدام وسط الاملاح المعدنية ا | Forty five local bacterial isolates were isolated from thirty different soil sample contaminated with pesticide, These samples were collected from different Baghdad regions at different periods. The bacterial isolates were tested for their ability to biodegrade certain pesticides (Malathion and Dursban). Primary and secondary screenings were carried out using solid Mineral Salts Medium (MSM) and Liquid (MSM) with 100 ppm Malathion and Dursban at 37 0C, pH 7. Twenty seven isolates resistant to Malathion and Dursban were isolated. Out of 27 bacterial isolates from different locations in Baghdad. Results from primary screening showed that the 10 isolates had good growth, 5 isolates gave moderate growth, 12 showed weak growth and only 9 isolates failed to show any growth.According to current results, 10 bacterial isolates were tested for their ability to biodegradation of Malathion and Dursban in liquid medium, However, secondary screenings results by using minimal inhibition concentration(MIC) revealed that only four bacterial isolates had the highest ability to degrade Malathion and Dursban and subsequently were identified as Pseudomonas putida, Esherichia coli, Escherichia hermanii and Staphylococcus vitulinus by Vitek compact system respectively.The optimum conditions (incubation period, pH, temperature) for growth and biodegradation of Malathion and Dursban were examined. The obtained results indicated that the best incubation period was after 7 days, pH =6 and favorite temperature was 350C.Measurement physicochemical properties for soil pH, temperature (C°), electrical conductivity EC (µs/cm) for both Dursban and Malathion concentrations(ppm) collected from six different locations within Baghdad city. Biodegradation of Malathion and Dursban in soil inoculated with different bacterial mono - cultures and mixed - cultures were investigated. soil pH, temperature, electrical conductivity, cations and anions, on the population of native Malathion - Dursban tolerant bacteria in the cultivated soils of six sites. The result showed that soil physical properties like temperature and electrical conductivity affect significantly to native Malathion - Dursban tolerant bacterial density. Similarly, the soil chemical properties like pH, cations and anions had more effect on the abundance of Malathion - Dursban tolerant bacteria in the soil. However, the impact of soil organic carbon, organic nitrogen and available phosphorus was very significant. The results of the present study can be utilized for the development of effective bioremediation process for pesticide - contaminated soil. Under optimal conditions, Malathion and Dursban degradation was measured by using GC - MS analysis. The results revealed that Pseudomonas putida was the best isolate for degrading Dursban by monoculture isolate (47.18%) , while mixed culture(P.putida and S.vitulinus) was the best in degrading Malathion and Dursban that gave 90.84% and 81.055% respectively. Results also indicated that the number of byproducts produced by mono and mixed culture of Malathion and Dursban biodegradation. Whereas the results obtained from GC/Ms analysis revealed that not detected of toxic byproduct Malathion and Dursban degradation by Pseudomonas putida and Staphylococcus vitulins respectively.Moreover, under natural conditions.The study tested the ability of selected bacterial isolates (P. putida and S. vitulins) in biodegradation of Malathion and Dursban, the results showed slower biodegradation by selected bacterial isolates compared with laboratory conditions but study showed a intermediet product like phenol, diethyl phosphorothioate and oxon.The GC - MS results showed that the number of metabolic intermediates formed during the degradation of Malathion by bacterial mixed - culture were relatively higher in number than by bacterial mono - culture.The mass spectra of malathion containing samples treated with bacterial mono and mixed culture showed no any known toxic intermediates. The result indicated that the two main degradation products resulted from bacterial degradation, namely malathion monocarboxylic (MMA) and malathion dicarboxylic acid (MDA), the first one may convert into the latter over time. Some other degradation products may occur such as ethyl hydrogen fumarate (EHF) but in negligible amount.The result indicated that the main degradation products resulted from bacterial degradation of dursban is trichloropyridinol(TCP) and then de - chlorinated into 2 - pyridinol.The mass spectra results obtained in this study showed that the dursban was degraded to many metabolites.The results suggested that the bacterial isolates were not forming any toxic intermediates but no any intermediate was identified till R.T. 22 minutes. This indicated that dursban is probably completely metabolized by the isolates into smaller intermediatesThe isolate Pseudomonas putida isolated from soil contaminated with pesticides was identified genotypic tests. Molecular typing was performed by RAPD - PCR and comparison of the results to other Pseudomonas isolates. The result shows that genotyping differences between isolates and found the convergent percent biodegradation between this isolates of both selected pesticide, although from these differences, there was no more effect to biodegradation, which means that the biodegradation of Malathion and Dursban not related to genotype

تاثير المدعمات المختلفة في حاصل ومكونات الفطر الغذائي Agaricus bisporus واختبار كفاءته في عدد من المتغيرات الكيموحيوية والفسلجية في اناث الجرذان == Effect of Various Supplementation in The Yield And Compositions of An Edible Mushroom Agaricus Bisporus And Evaluation of Its Efficiency in Some Biochemical And Physiological Parameters in Female Rats

اسم المؤلف: جمال جليل احمد البياتي
اسم المشرف: كركز محمد ثلج الجبوري | عبد الله عبد الكريم حسن
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: اجريت الدراسة في كل من مزرعة العرهون التابعة لقسم وقاية النبات - كلية الزراعة ومختبرات قسم علوم الحياة - كلية التربية للعلوم الصرفة في جامعة تكريت خلال المدة 1/9/2013 - 10/6/2014 تضمنت دراستين الاولى تم فيها تقدير تاثير اضافات متنوعة من المدعمات الكاربو | The study was conducted in each of the Al - Mushroom farm in Department of plant protection, College of Agriculture and the laboratories of Biology Department - College of Education for Pure Science at the University of Tikrit for a duration of 1/9/2013 to June 2014. The first studies were included the adding of carbon, nitrogen and other additives at casing period on productivity, quality characteristics and chemical composition of Agaricus bisporus mushroom. The results showed significant increased (p<0.05) in total production and biological efficiency when adding carbon supplement at 1% of starch and became 26.41 kg/m2 and 88.03% respectively, also with nitrogen supplement at 1% from Erbil feed (EF) that’s became at 23.20 kg/m2 and 77.33% respectively, compared with the same value in control group which at 21.68 kg/m2 and 72.26% respectively. Also both treatments were recorded earlier in production at 15 and 16 days after casing compared with 18 days in a control, while the qualitative characterizes has been the treatment of 3% from methionine the highest content of total antioxidants and became at 1975 µg/g dry weight compared to the 1572 µg/g dry weight in the control treatment, and the highest protein content was became at 40.32% in treatment of 1% of LB compared with 22.36% of their content in the control treatment. The treatment of 1% of EF was recorder as higher content from mineral of calcium, magnesium and iron at 390, 2574, 50.74 µg/g dry weight compared to the content in the control group at 290, 1683 and 37.64 µg/g respectively, and the treatment of sodium selenite 5% was recordes a higher content of selenium at 5.00 µg/g compared to the control treatment which at 4.00 µg/g dry weight. The treatment of EF also recorded a higher copper content at 108.60 µg/g dry weight compared to the control treatment which at 37.00 µg/g. The other study was apply to assessment the effects of replacing casein at 5, 10 and 15% by the Agaricus bisporus product from the treatments with starch and multi - vitamin and EF on some growth and physiological parameters in female rats feding for 28 days. The results showed that the presence of mushroom in food has caused a significant decline in weight. The present of mushroom powder at 5% in rats feed lead to significant increasing in hemoglobin and total account of RBC resulting in 12.53 and 6.36× 106 cell/mm3, 13.13 and 7.06× 106 cell/mm3, 7.36× 106 cell/mm3 for starch, multivitamins and EF, respectively, compared to 12.25 and 6.8× 106 cell/mm3, respectively. As well as the significant increased in the total counts of white blood cells that were at 5.50, 5.20 and 5.00 x 103 cells/mm3, respectively, with the starch, and at 5.70, 5.40 and 5.20 x 103 cells/mm3, respectively, with multivitamins and at 5.90, 5.50 and 5.40 x 103 cells/mm3, respectively, for the EF group compared with the control group were at 5.15 x 103 cell/mm3. also was significant increased in differential WBCs as lymphocytes with increasing the replacing percentage for treatments above. Also due the replace of casein by mushroom on proportions referred to in the above change in blood biochemicals parameters which significant decreased in urea, total cholesterol, triglycerides and low density lipoproteins and increase the concentration of high density lipoproteins, as well as reduced the activity of enzymes Alanine amino transferase (AST), Aspartic amino transferase (ALT) and Alkaline phosphatase (ALP) with increasing the percentage of replacing. As for the effect of replacing the same proportions of the mushroom instead of casein on growth and physiological characteristics of female rats with experimental diabetes induced by Alloxan and fed for 17 days. The results showed that the development of diabetes trial led to a reduction in the rate of animal weights and an increase in the relative weight of internal organs such as the heart, liver, spleen, pancreas and kidneys of infected sugar group, and became at 0.46, 4.39, 0.60, 0.46 and 1.19 g/100 g respectively when compared with the control animals, at 4.07, 0.42, 0.39, 0.23 and 1.04 g/100 g, respectively. And decreased of red blood cells that were at 5.93 x 106 cells/mm3, respectively, for the group affected by diabetes, while at 7.36 x 106 cells/mm3 respectively the healthy control group. And decreased WBCs in the infected animals with diabetes trial and became at 4.83 x 106 cells/mm3 compared with the healthy control group which at 7.03 x 103 cells/mm3. Also there was caused in significant increased in glucose concentration, which was on the first, 7th, 14th, and 17th days at 428, 405, 431.60 and 432.20 mg/100 ml respectively, in comparison with a control without diabetis that was at 123.33, 124.66, 126.33 and 125.33 mg/100 ml, respectively. Further to increased concentrations of urea, cholesterol, triglycerides and low density lipoproteins and a decrease in the values of high density lipoproteins also, increased the values of enzymes activity of each Alanine amino transferase(ALT), Aspartic amino transferase (AST) and Alkaline phosphatase (ALP) compared with the non - diabetic infected control group. That feeding female rats of experimental diabetes on the replacement rates of the mushroom instead of casein in their diet has led to the significant decline of the weight and relative weight of internal organs such as the heart, liver, spleen, pancreas and kidneys compared with animals infected with the induced diabetes with increasing rates of substitution. The results also showed that increased in total counts of red and white blood cells that was at 6.40 x 106 cell/mm3 and 5.00 x 103 cell/mm3 respectively when replacing 5% casein from mushroom compared to the infected control group were at 5.93 x 106 cell/mm3 and 4.83 x 103 cell/mm3 respectively. The substitution of casein with 5, 10 and 15% from mushroom was caused to significantly lower of glucose values to became 273.40, 251.60 and 302 mg/100 ml compared with control group infected which at 432.20 mg/100 ml. Also, because of the found of mushroom in the food was caused a changed in the biochemical blood, which significantly reduced the concentration of urea, total cholesterol, triglycerides, LDL and increase the concentration of HDL. As well as significantly reduced the enzymes (AST), (ALT) and (ALP) activity with the replacement rate increase compared with values in the animals infected with diabetes induced by Alloxan. The results concluded that the possibilty of use starch and EF at 1% for the commercial mushroom production. Mushroom also can be used as a food for lowering blood glucose, cholesterol, triglyseride, LDL and liver enzymes with increase in HDL for the people suffered from diabetes, obesity and heart diseases.

دراسة جزيئية ومناعية لطفيلي اللشمانيا الجلدية Cutaneous Leishmania في المحافظات الوسطى والجنوبية == Molecular And Immunological Study of Cutaneous Leishmania In The Middle And Southern Provinces

اسم المؤلف: ازهار موسى جعفر الموسوي
اسم المشرف: علي حسين الكبيسي | مهدي حسين العمار
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: علوم الحياة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:
المستخلص: The study includes distribution of the Cutaneous Leishmania in some governorates in Iraq. Specimens are included cases of outpatients in hospitals like : Al - Karamah, Al - Sadir Teaching Hospital in Baghdad, Al - Hilla Teaching Hospital, Al - Hussein Teaching Hospital and Ein Altamer General Hospitals in Kerbala, Al - Sadir Teaching Hospital in Al - Najaf, Al - Karamah and Al - Zahraa Teaching Hospitals in Wasit, Al - Diwanyia General Hospital. Al - Hussein General Hospital in Al - Nasiriya, Al - Smawa General Hospital, Al - Sadir Teaching Hospital and Al - Qurna General Hospital in Al - Basrah during October 1122 to march 1121 Cutaneous Leishmania is consider a major problem that faces the patients because it causes deformities in the infection region. The study includes 330 cases of skin ulcer in which 225 of them were microscopically positive. Parasitic growth was done using two types of cultural media NNN and RPMI - 1640.The results have shown a considerable difference between male and female in which male was significantly exceeding 131) %22.11 (and female was 94) %72.44 (and 139) %72.44 (of outpatients from rural areas increased in numbers from outpatients of urban areas, and only) %12.11(from urban areas.The number of ulcerations in body has exceeded infection, that means more than one ulceration in male 83 (85%) and for female 41 (62%), and concerning infection in three or four ulceration, it is approximately equal and normally distributed on face areas 99 and a percentage of (44%) then lower arms are 92 and a percentage of (40.80%) and the upper parts are 33 and a percentage of (14.66%). Also, one infection was recorded on The study shows that 46 patients in a percentage of (%20.44) having a dry infection; whereas 179 patients (%79.55) having the wet type.For accurate diagnosis of Leishmaniaa species distributed in Iraq, PCR Was used technique and diagnosed two types of Leishmania parasite that causes the illness. A band in a length of 560 bp in 186 samples related to Leishmania major and the band sample was 750 bp related to 39 Leishmania tropica.Through studying the samples in Kerbala City, the number infected samples were 125 for the years of 2010 - 2011 the actuality is (73) infection and (52) infection from 2011 - 2012 in a ratio of (58.4 % and % 72.7 accordingly) in different inhibited areas in the governorate in which (51)) %71.2 (was in Ein Al - Tamir Suburb, and 29 cases of) %11.1 (in Al - Husseinya Suburb, as well as 17 cases of (13.6%) in Al - Hur Suburb, 16 cases) %21.2 (in the south quarters and 7 cases of (5.6%) in the north quarters and only 5 cases of (4%) in the city center.The molecular diagnosis in Polymerase Chain Reaction for Kerbala governorate samples have shown 31) %17.2 (of L.tropica, 22) %24.7(of them were male and 9 of) %4.1 (of them were female. L. major was 94 of) %42.1 (in which 55 of) %77 (were in male and 39 of) %12.1 (were in female.The immunological study for the patients serums with L. major by ELISA technique has significantly shown raised values of IgG and IgM during infection in comparison with the control group, then it declines slowly after treatment in which IgG was in ratio (1811.1 ± 523.1 mg/dl), and IgM was in ratio (166.7 ±23.6 mg/dl). Also, in L. tropica - IgG was in ratio (1722.1 ± 524.0mg/dl) and IgM was in ratio (25.3±182.9mg/dl) in comparison with control specimens, and then the ratios have declined after treatment dosages with Pentostam drug.The cellular dynamics values have shown an abstract increase in which interferon - gamma (IFN - ?) in infected patients with cutaneous L. major were (221.1±2.2mg/dl) which declined after treatment abstractly to (7.12±1.1mg/dl) as well as for L tropica (22.1±7.2mg/dl) which shows no significant abstract difference after treatment with control.An increase appears in cytokine IL - 10 that reached in L.major (122.1±8.2mg/dl) and after treatment was (8.11±2.2mg/dl) and in L. tropica was (222.1±2.2mg/dl) with no significant abstract difference after treatment with control specimen.As a simple attempt to find a vaccine for Leishmania disease a Lipophosphoglycan was isolated and purified as known factor for promastigot stage. The vaccine injected for two groups of Bulb mice in a concentration of purified vaccine (L.majorand Ltropica) to study the immunological response by detecting the Lymphocyte Transformation assay, the Delayed Type Hypersensitivity test and the PhagocytosisIndex.In lymphocyte transformation the ratio of L. tropica was % 7.2 and for L. major was % 21.7 and there is significant difference by P? 0.05 in comparison with control specimen in which its ratio was %7.4, and for Delayed Hypersensitivity test study the average foot thickness injected with vaccine and the other foot which injected with phenol - saline solution - only (1.11 ± 2.12ml) for the first group and (1.21 ± 2.72) for the second group, and for the control group it has registered (1.77± 1.15 ml) with a considerable difference of P? 0.05. By calculating the ratio of Phagocytosis Index cells, it was 17% and 27.6% for the first and second groups while for the control group it has been reaching 9.10%. Therefore, we can get advantage from these values as indicators for the rule of vaccine in the immunological response and triggering of vaccine idea for this disease.

العلاقة بين تجارب الطفولة السيئة والمطاوعة على الصحة بعد البلوغ في بغداد == The Relationship Of Adverse Childhood Experiences And Resilience To Adult's Health In Baghdad City

اسم المؤلف: اميل فاروق الشاوي
اسم المشرف: رياض خضير لفتة | صالح الحسناوي
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: طب المجتمع
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تجارب الطفولة السيئة تشير الى اكثر مصادر الاجهاد شيوعا وشدة التي يعاني منها الاطفال في حياتهم المبكرة. من هذه الخبرات الاساءة، الاهمال، العنف بين الابوين او مقدمي الرعاية والعنف المجتمعي. وتبين ان الاجهاد الطويل في الطفولة له اثار طيلة الحياة على صحة | Adverse Childhood Experiences refer to some of the most intensive and frequently occurring sources of stress that children may suffer early in life. Such experiences include multiple types of abuse; neglect; violence between parents or caregivers and community violence exposure. It has been shown that prolonged stress in childhood has life - long consequences for a person's health and well - being; it may lead to serious problems such as alcoholism, depression, eating disorders, heart diseases, cancer, and other chronic diseases. Resilience is the ability to manage crises, difficult situations and developmental tasks. Research on resilience has identified several factors that strengthen the power and resources of children as well as promote their abilities to cope successfully with crises and internal and external problems. This cross - sectional study was conducted to estimate the effect of adverse childhood experiences and resilient factors on adults’ heath. It was done during the period from January 2013 through January 2014. The study sample was drawn from Baghdad City. Multistage sampling technique was used to choose 13 primary health care centers and eight colleges from three universities in Baghdad; the questionnaire was filled through a direct interview with the participants. In addition to that, teachers of seven primary schools and two secondary schools were chosen by a convenient method. A total of 1040 subjects were surveyed and 1000 responded. Their age ranged between 18 to 59 years (32.08±11.169). Females constituted a higher proportion (58.3%) of the study sample, 60% of this sample had university/diploma as the highest level of education. The results showed (during childhood) that seeing or hearing a parent or household member in home being yelled at, screamed at, sworn at, insulted or humiliated was reported in 469 (46.9%). A parent, guardian or other household member yelled, screamed or swear at, insulted or humiliated them was registered in 387 (38.7%). A parent, guardian or other household member spanked, slapped, kicked, punched the subjects was seen in 335 (33.5%). father's death (when the subject's age was 15 years or less) was reported in 104 (10.4%) of the participants, and 74.8 % of the subjects like to be the kind of people their parents were. The multiple linear regression models demonstrated that being in the fourth quartile (highest quartile) of household dysfunction - abuse score is expected to significantly increase depression symptoms score by a mean of (10.4) compared to subjects in the lowest (first quartile) of household dysfunction - abuse score, being in the fourth quartile of family bonding score is expected to significantly decrease depression symptoms score by a mean of ( - 22.5) compared to subjects in the first quartile, the same is correct for self - esteem score which increases by a mean of (21.484) for the fourth quartile of family bonding score compared to subjects with lowest (first quartile) after adjusting other explanatory variables. Logistic regression model showed that being in a higher level of family bonding (fourth quartile) is expected to reduce the risk of having chronic physical diseases by almost the half (odds ratio =0.57) and being exposed to a high level of household dysfunction and abuse (fourth quartile) is expected to increase the risk of having chronic physical diseases by 81%. A positive history of suicidal attempts showed a strong positive association with mean score of household dysfunction and abuse. Family bonding during childhood appears to be the most important resilient factor that protects adults against sedative or narcotic usage, mental disorders, suicide attempts, and chronic physical diseases during adulthood. Higher levels of exposure to adverse childhood experiences have a positive association with substances usage, suicide attempts, symptoms of mental disorders and chronic physical diseases in adulthood.

قياس وتفسير وامكانية الاستخدام السريري لتشتت فترات QT والمعلمات لتخطيط صدى القلب في مرضى مرض القلب الاقفاري == Measurement, Interpretation, And Use Of Clinical Potential Of QT Dispersion And Intervals With Echocardiographic Parameters In Patients With Ischemic Heart Disease

اسم المؤلف: فراس عبد الودود عبد العزيز
اسم المشرف: تقي علي موسى الموسوي الواجدي | بسام طالب فائق الكيلاني
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: الفيزياء الطبية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجري تخطيط القلب الكهربائي واخذت نتائج فحص الايكو لاربعة وخمسون مريض (اناث وذكور)، عمرهم المتوسط كان 51,1 ± 6,7 سنة؛ المدى كان يتراوح بين 35 - 66 سنة. المرضى الذي جندوا في الدراسة الحالية صنفوا الى اربع مجموعات : مرضى القلب الاقفاري الشرايين التاجية لوحده | ECG traces were recorded and echo findings were obtained for 54 patient (females were 15 and males were 39), their mean age was 51.1 ± 6.7 years; the range was 35 - 66 years. Patients that were recruited in the present study were classified into four groups : Patients with Ischemic Heart Disease (IHD) alone were labeled as group 1, Patients with IHD and Hypertension (HT) were labeled as group 2, Patients with IHD and diabetes mellitus (DM) were labeled as group 3, and Patients with IHD, HT, and DM were labeled as group 4. Fourteen age - matched apparently healthy subjects (mean age 48.6 ± 4.0 years) were chosen for comparison with group 1 patients. Patients' ECGs didn't show clear IHD before Treadmill test. Patients who showed clear ischemic changes within the treadmill test or during the rest time after completion of the treadmill test were sent to echo unit for structural / functional assessment of their hearts. Before stress test, simultaneous resting 12 lead ECG, arterial blood pressure, heart rate, height and weight of the patients were measured, age were recorded, smoking habit, and history of any cardiovascular disease. QT (The time interval measured from Q - wave onset the end of the T - wave), QT peak (The time interval measured from the Q - wave onset to the peak of the T - wave), TpTe (The time interval measured from the peak to the end of the T - wave)intervals were measured manually by tangent method on resting ECG. The dispersion of QT, QTp, TpTe intervals determined by the difference between minimal and maximal of these above measured intervals within 12 leads. The measured QT intervals were corrected for the subject's heart rate by using the standard Bazett's formula. In comparison with healthy subjects, QT dispersion (QTd) and QT peak dispersion (QTpd) of patients in group 1 were significantly higher (by 29% and 25% respectively) relative to their counterpart dispersion of healthy subjects, Tpeak - Tend dispersion (TpTed) of patients in group 1 was significant lower by 57% than TpTed of healthy subjects, QT interval and QTp interval of patients in group 1 was significantly longer (by 5% and 18% respectively) relative to their counterpart intervals of healthy subjects, in contrast, TpTe interval of patients in group 1 was significantly lower (by 27%) relative to their counterpart in healthy subjects. QT - intervals of patients in group 2, 3, and 4 are significantly higher (by 2%,4%, 3%) respectively than patients in group 1. QTp interval of group 3 was significantly higher than group 1, 2, and 4 by (4%, 6%, 4% respectively). No significant differences were observed in QTp interval among group 1, 2, and 4. TpTe interval of group 2, group 3, and group 4 patients were significantly longer by (16%, 6%, 14%) respectively relative to the TpTe interval in patients of group 1. QTd of patients in group 4 is significantly higher by (30%) than QTd of patients in group 1. QTp dispersion of group 4 is significantly higher by (26%) relative to QTp dispersion of group 1. TpTed of group 2 and group 4 were significantly higher (62% and 46% respectively) than TpTed of patients in group 1. LVMI of patients in group 2 and 4 were significantly higher (by 38% and 51% respectively) over the LVMI in patients of group 1. RWT of patients in group 2 and 4 were significantly higher (by 13% and 17% respectively) over the RWT of patients in group 1. PWT of patients in group 2, 3, and 4 was significantly higher (by 15%, 18%, and 21% respectively) over their PWT counterpart of patients in group 1. IVSTS was found to be significantly higher by (19% and 20%) of patients in group 2 and 4 respectively relative to patients in group 1. IVSTD was significantly higher by 24% in patients of group 4 only relative to the IVSTD of patients in group 1. IVSTS and IVSTD were significantly correlated with the QTd and QTpd in group 1. In group 2 patients RWT and PWT were significantly correlated with QTd and QTpd and IVSTD was significantly correlated with QT, QTp, and TpTe interval. In group 3 patients, IVSTD, ARD, and BMI were found to be significantly correlated with QTd and QTpd. significant correlation also was found in this group between RWT and QTp interval and IVSTS was correlated significantly with the QT and QTp intervals in group 4 patients.

دراسة تاثير اديبونكتين على هيكل كبد الفئران وعلاقته بالنظام الغذائي وكمية الدهون في الجسم == A Study Of Adiponectin Effects On The Mice Liver Architecture In Relation To Dietary Intake And Body Fat Content

اسم المؤلف: غسان علي عبد الحسین
اسم المشرف: سلمان شفیق سلمان | عماد غانم قاسم
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: التشريح والانسجة والاجنة
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يعتبر مرض الكبد الدهني غير الكحولي الاضطراب المزمن الاكثر شيوعا في الكبد واصبح تشخيصه في ازدياد مع زيادة وباء السمنة العالمي وقد تم تصميم الدراسة الحالية لدراسة الفوائد المحتملة للاديبونيكتين على النماذج الحيوانية من مرض.تم تطوير نموذجين من اضطراب الكبد | Non - alcoholic fatty liver disease is the most common chronic liver disorder whose prevalence has been on the rise with the worldwide increasing pandemic of obesity. The current study was designed to study potential benefits of the adipokine (adiponectin) on animal models of non - alcoholic fatty liver disease.Two models of the disorder were developed using two different approaches. In experiment 1 (25 mice, last 8 weeks), fatty change was induced using high fructose intake to simulate human - like diet induced obesity and fatty liver disease. In experiment 2 (25 mice, last 3 weeks), fatty liver damage was induced by using methionine - choline deficient diet. Animal body weights, absolute and relative liver weight, food consumption and fructose water consumption were recorded. Visceral (epididymal) fat and liver tissue were studied using routine hematoxylin and eosin staining and histochemical stains including Masson trichrome, Periodic acid schiff and reticulin stains. Morphological and morphometric changes were compared between animals treated with adiponectin, untreated diseased animals, and control healthy animals and a non - alcoholic steatosis activity score was used to evaluate the severity of liver damage. Random blood sugar and serum lipid levels were also included as biochemical metabolic parameters.High fructose intake resulted in larger visceral fat depots and weight gain, effects that were combated by adiponectin treatment despite increased food intake. Hepatic steatosis, inflammation, pyknotic pictures and fibrosis were reduced in adiponectin treated animals resulting in a lower activity score. Random blood sugar levels were normal in all animal groups but were significantly lower in adiponectin treated groups. TG and VLDL were alleviated with adiponectin treatment.Methionine - choline deficient diet resulted in emaciation of animals, much reduced visceral fat depots but a more severe form of liver fatty damage over a shorter period of time when compared to high fructose obese animal models. Weight loss was worsened after adiponectin treatment. Hepatic steatosis, inflammatory foci, ballooning degeneration and fibrosis were all exaggerated but remained significantly less severe with less activity score in adiponectin treated animals. Random blood sugar levels were normal in all animal groups but were significantly higher after adiponectin treatment. TG and VLDL was alleviated with adiponectin treatment.Adiponectin affects appetite, food consumption and body composition in healthy animals and animal models of non - alcoholic fatty liver disease through different mechanisms. It has beneficial effects in reducing body weight in obese animals but can modulate weight loss in lean animals on methionine - choline deficient diet. Adiponectin has autocrine/paracrine effects on visceral adipose tissue and can correct the dyslipidemic picture of fatty liver disease. The antisteatotic and antifibrotic effects of adiponectin carry greater therapeutic potentials in obesity - related liver disease.

تشخيص مصلي وجزيئي لفيروس الحلا البشري النوع السادس المصاحب لبعض سرطانات الدم == Serological And Molecular Detection Of Human Herpesvirus Type 6 Associated With Certain Hematologic Malignancies

اسم المؤلف: هديل محمد فياض
اسم المشرف: علاء فاضل علوان | داود سلمان داود
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Human herpesvirus 6 (HHV - 6) is largely ubiquitous double stranded DNA viruses within the betaherpesvirinae subfamily and of the genus Roseolovirus.HHV - 6A and HHV - 6B infects very high percentage of population around the world, primarily during childhood through respiratory droplets. HHV - 6 has been found out in many types of cancers : lymphomas, leukemia, cervical cancer, and brain tumors.This cross - sectional case control study was carried out in Baghdad Teaching Hospital and National Center of Hematology - AL - Mustansiriyah University - Baghdad, for the period from 1 September 2013 to 1 April 2015.The aims of the study were to explore the seropositivity rate of HHV - 6 antibodies among Iraqi patients with different hematological malignancies using different laboratory assays, beside, the molecular determination of plasma viral DNA load by quantitative polymerase chain reaction (qPCR).The study included 109 patients with different hematological malignancies; 24 patients with acute lymphoblastic leukemia (ALL), 30 patients with acute myeloid leukemia (AML), 11 patients with Hodgkin lymphoma (HL), 39 patients with non hodgkin lymphoma (NHL) and 5 patients with chronic lymphocytic leukemia(CLL). All patients were newly diagnosed and enrolled before receiving chemotherapy. The age range was between 14 - 80 years. Fifty three (48.6%) and 56 (51.4%) patients were males and females, respectively. The diagnosis of their malignancies was based on either bone marrow aspirate biopsy and lymph node biopsy as well as cytochemical investigations. Additionally, 59 apparently healthy blood donors were enrolled as control group. The age range was between 18 - 59 years, twenty eight (47.4%) and 31 (52.6%) were males and females, respectively.The serum anti - HHV - 6 IgG was detected by indirect immunofluorescent technique (IFAT) as well as by enzyme linked immunosorbant assay (ELISA).While, the serum anti - HHV - 6 IgM was detected by ELISA only. The detection and quantification of plasma viral DNAemia was carried out by quantitative polymerase chain reaction. Serological as well as molecular assays were done in Central Public Health Laboratory in Baghdad. The highest IFAT positivity rate was among patients with CLL (100%), followed by patients with AML (83.3%), and the least positivity rate was among patients with NHL (64.1%) compared to that of healthy control. Generally, the total anti - HHV - 6 IgG by IFAT was insignificantly higher among patients compared to healthy controls (74.3% vs 61.0%, p=0.074). The anti - HHV - 6 IgG positivity rates by ELISA were insignificantly higher in all groups of haematological malignancies except in patients with AML in whom it was significantly higher compared to healthy controls (96.7% vs 72.9%, p= 0.007 ). A part from AML, the highest anti - HHV - 6 IgG by ELISA was among patients with ALL (87.5%) followed by patients with NHL (84.6%), and the least was among patients with CLL (80.0%) compared to healthy control. Generally, the total anti - HHV - 6 IgG positivity rate detected by ELISA was significantly higher compared to IFAT in patients (p= 0.013) as well as in healthy control (p= 0.0005).The results of anti - HHV - 6 IgM positivity rate as detected by ELISA was significantly higher in patients with AML (36.7%, p=0.0004), and patients with HL (27.7%, p= 0.038) compared to healthy controls (6.8%). Whereas, other groups of hematological malignancies showed insignificantly higher positivity rate versus control group. However, the highest positivity rate was among patients with AML (36.7%), and the least positivity rate was among patients with CLL (0%). Generally, the total ELISA anti - HHV - 6 IgM positivity rate was significantly higher in patients versus healthy controls (22.0% vs 6.8%, p= 0.011). The results of PCR showed that 5 out of 109 patients had detectable HHV - 6 DNA in the plasma. The highest detection rate was among patients with HL (27.3%), followed by patients with ALL (4.2%), and then patients with AML (3.3%), while the virus was not detected in other disease categories as well as in healthy controls. The plasma viral load (mean ± SD) among the three Hodgkin lymphoma patients was 1.4± 0.3*102 particle/milliliter.The study concluded that the seropositivity rate of HHV - 6 infection is highly prevalent among Iraqi patients with hematological malignancies compared to healthy population. However, primary or reactivation infection as determined by anti - HHV - 6 IgM in patients as well as healthy individuals is relatively low. Furthermore, the HHV - 6 DNA detection rate as well as plasma viral load among malignant patients before receiving immunosuppressive treatment were low.

دراسة Toll like receptors(TLR2& ( 4 ومؤشرات حيوية مختارة في المرضى المصابين بسرطان المثانة == Study Of Toll Like Receptors (TLR - 2 And 4) And Selected Biomarkers In Bladder Cancer Patients

اسم المؤلف: هدى سعدون البياتي
اسم المشرف: ميسون علي سليم | ناهي يوسف ياسين | عصام سلمان العزاوي
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Worldwide, cancers of the urinary bladder are complex and involve genetic abnormalities and may be due to different environmental chemical carcinogens, as well as chronic infection like Schistosomiasis, all these will allow normal transitional cells to become cancerous which are typically transitional cell carcinoma(TCC).This study was conducted at AL - Yarmouk and Baghdad Teaching Hospitals in Baghdad from June 2013 to April 2014, of which urine and bladder tissue were taken from 57 bladder cancer patients and 80 controls to assess Interleukin - 8 (IL - 8), Matrix MetalloPeptidase - 9 (MMP - 9), Bladder Cancer - Associated Protein (BLCA - 4),Toll - Like Receptors (TLR - 2and TL - R4) level among them and to evaluate their roles in cancer development.One hundred thirty seven Iraqi individual were divided to 28(20.43%) newly diagnosed and 29(21.16%) recurrent bladder cancer patients (relapse), 40(29.19%) apparently healthy volunteers and 40(29.19%) autopsies (apparently normal urothelium), of whom urinesample and tissue biopsy were collected, clinical diagnosis of patients carried out by cystoscopic and histopathological examination, the mean ±SD age of bladder cancer patients were (63± 9.3) with (M : F ratio7.1 : 1).Predominant cancer type was Transitional cell carcinoma (TCC). According to risk factors of bladder cancer, out of 57 bladder cancer patients, 34(59.64%), 29(50.87%), 9(15.78%), 7(12.28%), 6(10.52%) were smoker, had UTI, history of Schistosomiasis, family history ofcancer and stones respectively. In this study 30 patients had non - muscle invasive bladder cancer, most of them (21) had newly diagnosed tumors, and only 9 had recurrent disease, as well as, 27 patients with muscle - invasive bladder tumor, just 7 had newly diagnosed tumors and 20 with recurrent disease.According to the result of ELISA technique, the mean±SD urine level IL - 8(pg/ml) for recurrent bladder cancer patients was 359.6±170.1pg/ml, for newly diagnosed was 265.95±139.09pg/ml and for healthy volunteers was 62.04±37.66pg/ml. The mean ±SD concentration of urine MMP - 9(pg/ml) was 7368.3±1730.19pg/ml for recurrent, 6176.8±2366.9 pg/ml for newly diagnosed and 1131.53±1554.80 pg/ml for healthy volunteers. Mean±SD Level of urine BLCA - 4 (ng/ml) for recurrent was 1.45± 0.26 ng/ml, for newly diagnosed was 1.30± 0.22 ng/ml and for healthy volunteers was 1.02±0.067 ng/ml. Urine level of these markers was significantly higher in recurrence group when compared with newly diagnosed and these levels decreased in normal volunteers which was statistically significance (P<0.001 for IL - 8, MMP - 9and BLCA - 4),also there was a significant increase in urine IL - 8 level according to grade(P=0.0002) and muscle invasion(P= 0.0009) as well as BLCA - 4 showed this significant increase in high grade(P=0.0001), and muscle invasion(P=0.0001),but no significant difference was found in the level of urine MMP - 9 with grade(P=0.23) and muscle invasion(P =0.069)In comparison, IL - 8 and MMP - 9 IHC staining of bladder tissue, showed low expression in cancerous tissue with no significant difference between cancer and normal urothelium(P=0.140 for IL - 8, P=0.265 for MMP - 9 ), and unrelated to grade, muscle invasion and recurrence.Significant increase in TLR - 2 and TLR - 4 expression in bladder cancer tissue than in normal urothelium (P=0.0001 for each), and according to grade and muscle invasion, TLR - 2 showed significant over expression in high grade than low grade (40.7%vs36.6%) with P=0.018, muscle invasion than non - muscle invasion (44.4%vs33.3%) with P value of 0.02, while TLR - 4 was none significantly correlated with grade andmuscle invasion. As well as, there was no correlation between these receptors with tumor recurrence.In regard to risk factors, Smoking, schistosomiasis and family history showed correlation with study marker in different manner as, IL - 8 was significantly highly expressed in smoking associated bladder cancer group, as well as patients with family history of bladder cancer showed high expression IL - 8, for Schistosoma associated TCC patients and UTI they demonstrated a statistically significant high expression of TLR - 2 p= (0.0001).From this study we conclude that urine IL - 8, MMP - 9and BLCA - 4 measured by ELISA showed specificity in diagnosis of bladder cancer, besides that, urine IL - 8, MMP - 9 and BLCA - 4 have a role in discrimination between newly diagnosed vs recurrent, with a significant association between urine IL - 8 with BLCA - 4 (P= 0.0001) and IL - 8 with MMP - 9 (P= 0.005) in patients with recurrent bladder cancer. Urine IL - 8 and BLCA - 4 concentration were statistically with significant increase in high grade than low grade and muscle invasion than non - muscle invasion, On the other hand, bladder cancer cells over express TLR - 2 and TLR - 4,while TLR - 2 showed correlation with high grade and muscle invasion,TLR4 did not show such correlation. Lastly, IL - 8 and MMP - 9 staining showed low expression in tumor tissue of different grades, stages and groups.
1 ... 56 57 58 59 60 ... 76