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دراسة مناعية ووراثية تتبعية لمرضى العقم من الرجال == A Prospective Immunological And Genetic Study of Infertile Men
Author name:
علي عبد الزهرة مهدي الفحام
Supervisor name:
يحيى كاظم السلطاني | عبد الزهرة كاظم محمد علي
General topic:
Biology
Specific topic:
Zoology - Physiology
Degree:
Doctorate
University:
University of Kufa - College Of Science - Department Of Biology
Language:
English
University location:
Najaf
First pages:
24T2702 - p.pdf
Abstract:
ان من الحقائق العلمية الثابتة اليوم ان الفحص الروتيني للسائل المنوي يعد عاملا تنبؤيا اجماليا للقدرة الاخصابية للرجال، ولذلك فان حاجة متزايدة بدات تبرز لاكتشاف فحوصات وظيفية جديدة في عملية تقييم العقم عند الرجال. ان الهدف الاساس من هذه الدراسة هو تعزيز الج | It is a scientific fact today that routine seminal fluid analysis is a key predictor of male reproductive potentiality ; so that there is an increasing need for finding out other functional tests in the assessment of male infertility. The main goal of the current study is to consolidate the scientific and practical efforts concerned with male infertility assessment especially from immunological and genetic aspects. The study was carried out between January 2013 and December 2013 including one hundred (100) selected infertile men who attended the Fertility Center in al - Sadr Medical City in Al - Najaf Al - Ashraf Governorate, the study also included twenty (20) healthy volunteer fertile men as a control. Serum and seminal antisperm antibodies (ASA) were determined by ELISA (Enzyme Linked ImmunoSorbent Assay) technique ; sperm chromatin condensation was evaluated by aniline blue staining (AB) ; sperm DNA fragmentation (damage) was evaluated by toluidine blue staining (TB). The results showed that the incidence and concentration of serum and seminal plasma ASA in the infertile men were significantly (p<0.05) higher than that in control fertile men. The incidence and concentration of serum and seminal ASA were also significantly (p<0.05) higher in normozoospermic infertile patients than that in control fertile men. There was a high significant negative correlation (p<0.01) between the concentration of serum and seminal plasma ASA in the infertile men and sperm motility and progressive motility, the concentration of serum and seminal ASA also showed a high significant positive correlation (p<0.01) with sperm agglutination, and a significant positive correlation (p<0.05) with seminal WBC count. The results also revealed that there were no significant effects (p>0.05) for patients' age, infertility duration, ABO blood groups and smoking habits on the levels of ASA in the serum and seminal fluid, while higher significant increase (p<0.01) in serum and seminal ASA concentrations was observed in infertile patients with varicocele as compared with those without. The results have also revealed that the percentage of sperm with chromatin decondensation and DNA damage has shown a high significant (p<0.01) increase in infertile patients compared to fertile men. There was also a significant increase (p<0.05) in sperm DNA damage in normozoospermic infertile patients comparing with fertile men, but no significant difference (p>0.05) was found in sperm chromatin condensation between normozoospermic patients and fertile men. Both sperm chromatin decondensation and sperm DNA damage showed a significant positive correlation (p<0.05) with sperm morphology, and a significant negative correlation (p<0.05) with sperm concentration, motility and progressive motility. The present study also revealed that the level of chromatin decondensation has been affected by patients' age, infertility duration, smoking habits and varicocele. It was observed that the higher percentage of chromatin decondensation was recorded in patients older than 37 years, and in patients subgroups with infertility duration more than 15 years, it was also higher in smoker as compared with non - smoker patients and in those with varicocele as compared with those without. Similarly, the level of sperm DNA damage has also been affected by patients' age, smoking habits and varicocele. It was observed that the highest percentage of sperm DNA damage was recorded in patients older than 37 years. The highest level of sperm DNA damage was also seen in smoker patients as compared with non - smokers and in those with varicocele as compared with those without. In contrast, the effect of infertility duration on sperm DNA damage was not statistically different (p>0.05). The correlation and regression results recorded high significant correlations (p<0.01) between serum ASA and seminal plasma ASA, and between sperm chromatin decondensation and sperm DNA damage. However, there was no significant correlation (p>0.05) between the level of serum and seminal ASA and each of sperm chromatin decondensation and sperm DNA damage. The current study concluded that the defects in sperm chromatin status (chromatin decondensation and DNA damage), and the immunological disorders caused by serum and seminal plasma ASA may - at least partially - contribute to the etiology of infertility of the patients under study, even in those with normal seminal parameters. However, it seems that ASA affect fertility in a pathway that is different from that affected by sperm chromatin defect. It was recommended that both sperm chromatin staining techniques and ASA tests could be routinely used as complementary tests to diagnose infertility.
