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دراسة تاثير اديبونكتين على هيكل كبد الفئران وعلاقته بالنظام الغذائي وكمية الدهون في الجسم == A Study Of Adiponectin Effects On The Mice Liver Architecture In Relation To Dietary Intake And Body Fat Content
Author name:
غسان علي عبد الحسین
Supervisor name:
سلمان شفیق سلمان | عماد غانم قاسم
General topic:
Medicine
Specific topic:
Anatomy and Histology and Embryos
Degree:
Doctorate
University:
Mustansiriyah University
Language:
English
University location:
Baghdad
First pages:
19T1158 - p.pdf
Abstract:
يعتبر مرض الكبد الدهني غير الكحولي الاضطراب المزمن الاكثر شيوعا في الكبد واصبح تشخيصه في ازدياد مع زيادة وباء السمنة العالمي وقد تم تصميم الدراسة الحالية لدراسة الفوائد المحتملة للاديبونيكتين على النماذج الحيوانية من مرض.تم تطوير نموذجين من اضطراب الكبد | Non - alcoholic fatty liver disease is the most common chronic liver disorder whose prevalence has been on the rise with the worldwide increasing pandemic of obesity. The current study was designed to study potential benefits of the adipokine (adiponectin) on animal models of non - alcoholic fatty liver disease.Two models of the disorder were developed using two different approaches. In experiment 1 (25 mice, last 8 weeks), fatty change was induced using high fructose intake to simulate human - like diet induced obesity and fatty liver disease. In experiment 2 (25 mice, last 3 weeks), fatty liver damage was induced by using methionine - choline deficient diet. Animal body weights, absolute and relative liver weight, food consumption and fructose water consumption were recorded. Visceral (epididymal) fat and liver tissue were studied using routine hematoxylin and eosin staining and histochemical stains including Masson trichrome, Periodic acid schiff and reticulin stains. Morphological and morphometric changes were compared between animals treated with adiponectin, untreated diseased animals, and control healthy animals and a non - alcoholic steatosis activity score was used to evaluate the severity of liver damage. Random blood sugar and serum lipid levels were also included as biochemical metabolic parameters.High fructose intake resulted in larger visceral fat depots and weight gain, effects that were combated by adiponectin treatment despite increased food intake. Hepatic steatosis, inflammation, pyknotic pictures and fibrosis were reduced in adiponectin treated animals resulting in a lower activity score. Random blood sugar levels were normal in all animal groups but were significantly lower in adiponectin treated groups. TG and VLDL were alleviated with adiponectin treatment.Methionine - choline deficient diet resulted in emaciation of animals, much reduced visceral fat depots but a more severe form of liver fatty damage over a shorter period of time when compared to high fructose obese animal models. Weight loss was worsened after adiponectin treatment. Hepatic steatosis, inflammatory foci, ballooning degeneration and fibrosis were all exaggerated but remained significantly less severe with less activity score in adiponectin treated animals. Random blood sugar levels were normal in all animal groups but were significantly higher after adiponectin treatment. TG and VLDL was alleviated with adiponectin treatment.Adiponectin affects appetite, food consumption and body composition in healthy animals and animal models of non - alcoholic fatty liver disease through different mechanisms. It has beneficial effects in reducing body weight in obese animals but can modulate weight loss in lean animals on methionine - choline deficient diet. Adiponectin has autocrine/paracrine effects on visceral adipose tissue and can correct the dyslipidemic picture of fatty liver disease. The antisteatotic and antifibrotic effects of adiponectin carry greater therapeutic potentials in obesity - related liver disease.
