Author name:
دعاء عبد الزهرة دلي الفتلاوي
Supervisor name:
عبد الهادي عباس الابراهيمي | سهاد حميد حسن
University location:
Najaf
Abstract:
العكبر (صمغ النحل) Propolis هو نتاج نحل العسل الذي اكتسب شعبية في الطب البديل وذلك بسبب خصائصه الحيوية، وقد استخدم بشكل واسع في الاطعمة الصحية. ان الدراسات المتعلقة بتاثير العكبر العراقي قليلة. ولهذا السبب, فان الهدف من هذا البحث هو تحليل تاثير العكبر الع | Propolis is a honeybee product that has gained popularity in alternative medicine, due to its biological properties and it has been intensively used in health foods. Studies concerning the effect of Iraqi propolis are rare. Therefore, the goal of this work is to analyse the effect of Iraqi propolis on some hematological and biochemical parameters in alloxan - induced diabetic rabbits, as well as study the histological observations of the pancreas, liver and kidney. Female local rabbits (Oryctolagus cuniculus) were used for this study. The overall number of animals used was 30.They were randomly divided into five groups. Diabetes was induced in all rabbits, except normal control, by a single dose of alloxan (150 mg/kg, i.v.). Development of induced diabetes mellitus was confirmed on first week after alloxan administration by examining the fasting glucose level in the blood taken from marginal ear vein. Rabbits with glycaemia were treated with alcoholic extract of propolis for 23 days. Diabetic control group did not treat with propolis. The treated animals were subdivided into three groups according to the dose of propolis extract. Three oral concentrations of propolis extract were investigated (50, 100 and 200 mg/kg/day).The following parameters have been studied were changes in weights of body, liver and kidneys ; changes in the hematological values that include erythrocytes, hemoglobin, leukocytes and hematocrit also changes in the biochemical values which included glucose, total protein, triglycerides, total cholesterol, aspartate transaminase (AST), alanine transaminase (ALT), blood urea, creatinine and Malondialdehyde (MDA), in addition histopathological study of pancreas, liver, and kidneys. The results indicate a significant decrease (P < 0.05) in the body weight of alloxan - induced diabetic rabbits in comparison with control group, while there were significant increases in the weights of liver and kidneys. The present study showed that alloxan induced significant decreases (P<0.05) in all primary blood indices; erythrocytes, hemoglobin, packed cell volume (hematocrit) and leukocytes. Also, serum biochemical changes showed significant increases (P<0.05) in glucose, total protein, triglycerides, total cholesterol, AST, ALT, blood urea, creatinine, and MDA comparison with control group.Histopathological changes in pancreas, liver and kidneys, observed microscopically, revealed degrees of damage in the tissues, while these organs of control groups exhibited a normal architecture. The treatment with alloxan resulted in several forms of histological alterations such as cytoplasmic vacuolation, degeneration, necrosis, cell hypertrophy, nuclear diploid, diffusion of inflammatory cells, dilatation in ducts, stagnation in secretory fluid. in addition, hemorrhage, dilatation and congestion in blood vessels and disorganization of histologic architecture, Generally, the gradual improvement in blood values was noticed with the increase in concentration alcoholic extract of propolis and return back the normal histological shape of pancreas, liver and kidneys. Propolis extract in rabbits had a potent antihyperglycemic effect, antioxidant activities, radical - scavenging capacities, tissue regeneration properties, and that may be due to the high biological activity and nutritive values contents in bee propolis. In conclusion, the results suggest that propolis could potentially contribute for the prevention and treatment of diabetes mellitus.
