البحث — المستودع الرقمي العراقي

نتائج البحث: 25 من أصل 140

دراسة بعض المؤشرات المناعية لدى بعض المرضى المصابين بفيروس المليساء المعدية في محافظة ديالى == A Study of Some Immunological Parameters In Some Patients With Molluscum Contagiosum In Diyala Province

اسم المؤلف: رغد ياسين اعويد

اسم المشرف: عباس عبود فرحان الدليمي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة ديالى - كلية التربية للعلوم الصرفة - قسم علوم الحياة

اللغة: الانكليزية

مكان الجامعة: ديالى

الصفحات الاولى:

المستخلص: المليساء المعدية مرض يسببه فيروس (MCV) الذي ينتمي لعائلة POXVIRUS.المليساء المعدية حيث انه مرض غير قاتل وشائع في جميع انحاء العالم, قد يشترك مع اورام المليساء التهابات قليله, المليساء المعدية يستمر لشهور او سنوات..اجريت الدراسة الحالية للفترة من 1 تشرين ا | The molluscum contagiosum (MC) virus (MCV) is a dermatotropic poxvirus, and the causative agent of MC. MC is nonlethal, common andworldwide. Additionally, little inflammation is associated with MCpapules, and MC can persist for months to years. The present study was conducted for the period from1 November 2013 to 30April, of 2014 in outpatient clinic of Baquba Teaching Hospital in city. The study aimsat assessing the immune status of patients infected with molluscumcontagiosum through the measurement ofthelevel of immunoglobulin(IgG, IgM)and the level of complementcomponents especially (C3, C4) by radial immune diffusion assay and measurement of the level of interleukin 18 R1 by Elisa assay which immune responses are key for the eventual resolution of MC. 75 patients were diagnosed with clinical lesions of MCV on different areas of the body, age of patients ranged from(2 - 50 years) including 40(53.3%) males and 35(46.7%) females.The control were 26.6±15.4 years (range from 2 - 50 years), 8 (53.3%) of them were males and 7(46.7%) females.After theexamination by single radial immune diffusion and Elisa kit.The results showed the patientswere equal or less than 16 years, 24 (32%) from 17 - 30 years, 19 (25.3%) from 31 - 45 years and 12 (16%) above 45 years and 40(53.3%) males and 35(46.7%) females, no static significant difference showed between the MCV infection and either the sex or age.The results revealed the level of IL 18R1in patient the mean±SD were 677.15±874.22 ng/ml while in control were 178.46±31.79 ng/ml. There was significant statistical difference between both groups as patients with MC had high level of IL18R1 than control.Themean±SD of IgMinpatients were 1946.6±825.6 mg/dlwhile in control were 140.1±68.7mg/dl This result was highly significant which indicates that patients with MC had higher level of IgM than control In contrast, patients had lower levels of IgG than control, The mean±SD of IgG in patientwere 221.9±96.7mg/dl while in control were 1229.9±299.7mg/dl. This result was highly significant. Also the patients had lower level of C3 and C4 than control. The mean±SD of C3 in patients were 109.6±64.8mg/dl while in control were 120.8±22.1mg/dl. The mean±SD of C4 in patients were 27.8±12.7mg/dl while in control were 38.7±9.8mg/dl. These results were statistically significant. Eleven 9 (14.7%) of patients had positive CRP while 12 (80%) of control had positive CRP which was statistically highly significant. Forty seven (62.7%) patients were from rural area while 28 (37.7%) from urban. There is no statistical difference between both groups as. Family history of MC and Recurrence of MC in patients were nostatically significant difference to infection by MCV.

دراسة بكتريولوجية لبعض الاجناس البكتيرية السالبة لصبغة غرام المقاومة لمضادات البيتالاكتام والمعزولة من اخماج المجاري البولية في محافظة ديالى == Bacteriological Study of Some Genus For Bacterial Gram - Negative Resistant To ? - Lactam, Isolated From Urinary Tract Infections In Diyala Province

اسم المؤلف: محمد خضير عباس النعيمي

اسم المشرف: عباس عبود فرحان الدليمي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الاحياء المجهرية - البكتريا

الدرجة: ماجستير

الجامعة: جامعة ديالى - كلية التربية للعلوم الصرفة - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: ديالى

الصفحات الاولى:

المستخلص: شملت الدراسة 300عينة جمعت من مرضى مصابين باخماج المجاري البولية وتم جمع العينات في مدينة بعقوبة من مستشفى البتول للولادة والاطفال ومستشفى بعقوبة التعليمي، للمدة بين 1/9/2013ولغاية1/1/2014.اظهرت نتائج الزرع البكتيري على اوساط اكار الماكونكي واكار الدم ووس | Three hundred urine from patients suffering from urinary tract infection from Baaquba Teaching Hospital and Al - Batool Hospital in Baaquba city for the period from 1/09/2013 to 1/01/2014. The results reved that 66 isolates are belonging to bacteria of Gram negative (57.4%), 25 (37.78 %) Escherichia coli, 22(33.33%) Proteus mirabilis, 9(6.06%)Klebsiella pneumonia, 5(7.57%) Enterobacter spp for 4 (25%) Enterobacter cloacae and 1(5.51%) Enterobacter aerogenes and5(7.57%) Pseudomonas aeruginosa by using diagnostic phenotypic, biochemical tests and confirm the diagnosis using regular aPI20E. Investigation of some virulence factors showed that both Escherichia coli and Proteus mirabilis produced haemolysin 52% and 90.9% respectively. The production of biofilm by local isolates was detected in Congo - red way, Isolates of Escherichia coli and Proteus mirabilis had been shown the ability to produce biofilm 92% and 90.9% respectively. The results showed that all isolates of Proteus mirabilis produced urease by 100%, while the results detect that the isolates of Escherichia coli not able to produce this enzyme. Proteus mirabilis showed ability to produce swarming with 100%. Siderophore production by Escherichia coli and Proteus mirabilis was 48%, 9% respectively. Eschrichia coli, and Proteus mirabilis produced bacteriocin with percentag of 32% and 50% respectively.The production of ? - lactamase by Eschrichia coli and Proteus mirabilis was 60% , 40.9% respectively, also the isolated had the ability to produce the Extendended spectrum ? - Lactamase enzyme by using disc Approximation, The production from each ofEschrichia coli and Proteus mirabilis 12%, 31.8 % respectively. Results of Metallo? - Lactamase by using the Imp - EDTA combination indicated that E.coli and P.mirabilis were 12% and 13.6% respectively. Isolates of E.coli showed highest resistance rate 92% for Augmentin while isolates of P.mirabilis showe higher resistant to cefotaxime 81.8%. The results showed that multiple resistance pattern for antibiotic 43 (91.5%) resistant to (2 - 5) antibiotics, Isolates of E.coli showed highest multiple resistance rate 92% while isolates of P.mirabilis rate 90.9%. The Minimal inhibitory concentration (MIC) for namely two antibiotics Cefotaxime, Ceftazidime were determined.MIC values of these antibiotics ranged between (2 - <1024), (4 - <1024) µg\ml E.coli and (2 - <1024), (8 - <1024) µg\ml P.mirabilis respectively. The results of molecular detection of ESBL genes (bla TEM and bla SHV) by using PCR technique, (9) samples from (10) total, divided into 3(100%) E.coli and 6(85.7%) P.mirabilis were harboring bla TEM gene based on the presence of 950 bp bands in 1% agarose gel. while results detect that the isolates of Escherichia coli and Proteus mirabilis not harboring bla SHV gene.

دراسة التغايرات النسجية والمناعية لمرضى اللشمانيا الجلدية في محافظة ديالى == Study The Histological And Immunological Variation of Cutaneous Leishmaniasis Patients In Diyala Province

اسم المؤلف: منى حميد احمد العزاوي

اسم المشرف: اسراء قاسم العبيدي

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الاحياء المجهرية - الطفيليات

الدرجة: ماجستير

الجامعة: جامعة ديالى - كلية التربية للعلوم الصرفة - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: ديالى

الصفحات الاولى:

المستخلص: اجريت الدراسة الحالية على المرضى المصابين بداء اللشمانيا الجلدية والمراجعين لبعض المستشفيات والمراكز الصحية في محافظة ديالى للمدة من تشرين الاول 2013 ولغاية نيسان 2014. اذ بلغ عدد المصابين باللشمانيا الجلدية 115 مصابا وبمختلف المراحل العمرية يضاف لهم 25شخ | The present study was conducted on patients that infected with cutaneous leishmaniasis reviewing to some hospitals and health centers in Diyala province for the period from October 2013 to April 2014.The number of patients with cutaneous leishmaniasis was 115 in different ages in addition to them 25 person as a control group (uninfected). The proportion of infected women with cutaneous leishmaniasis ulcers was higher 56.522% than males 43.47%, the age group ten years and less was more group that being infected with rate 47.83% while the age group that rang from 71 to 80 was the less categories being infected in percentage 0.87% with a significant differences in the level of improbability less than 0.05. The presence of multiple ulcers was high 71.304% than single ulcers 28.696%. The presence of ulcers that length 1 cm and less (64%) more than other with more than 1 cm (36%) with statistically significant. The cutaneous ulcers were distributed on different areas of the body, the injuries on face was more 36.242% with anumber of 54 cases followed by upper limbs in rate 33.557% with anumber of 36 cases, the sore shoulder came latter with apercentage 1.342% in a significant differences at the level of improbability less than 0.05. The percentage incidence of cutaneous leishmaniasis was rise in kids and students in rats 33.043%, 39.130% respectively in a comparison with earners, housewives and of officers their percentages were 24.349% and 3.478% consecutively in statistically differences at 0.05 probability. The diagnosis of cutaneous leishmaniasis infections was done depending on clinical diagnosis based on the characteristics of the morphological and pathological ulcers, culture methods, impression smears and histopathological diagnosis. Clinical diagnosis for the cutaneous ulcer was recorded 100% percentage in comparison with culture method 10.435% versus 74.782%. percentage to positive smears, while the result of positive histopathological diagnosis was 88.571% on vision of promastigote of Leishmania parasite on semisolid medium while a adoption of existence amastigote from as appositive result of smears and histopathological method of the tissue biopsies taken from ulcer. The histopathological method was high efficiency supported clinical diagnosis when compared to other methods this rise in the diagnosis ratio has been demonstrated in significant differences in statistical probability less than 0.05.A study of amain impacts of histopathological changes in cutaneous ulcers on the infected skin layers at the level of light microscopy was also conducted. These pathological effects were in epidermis of skin including hyperkeratosis, pseudoepitheliomatous epidermal changes, ulcers and emergence of a dark colour, thickening in this layer accompanied suppurations and desquamation of chronic cases. As for the dermis layer, the inflammatory cells were infiltrate include plasma cells, macrophages, lymphocytes and eoisophils, disturbance in the order of layers especially in acute ulcers was taken, while the pathological effects were more in chronic ulcers as observed granulomatous inflammation and necrosis. The amastigote from were presence inside and outside the phagocytic cells.Aswell as some immunological changes that associated with the infection were studied in patients therapists by Pentostame (Sodium Stubogluconate) medication and uninfected control group by measuring the levelsof some cytokines IFN - ?, TNF - ?, IL - 12, IL - 17A and the chemokine MCP - 1. The level of IFN - ? was elevated in serum of cutaneous leishmaniasis patients and reached to 54.118±1.740 pg/ml, then the level was decreased during therapy and recorded 34.421±0.984 pg/ml after 1 - 3 dose a then dropped more after 4doses and more 22.489±0.795 pg/ml compared to uninfected control group 5.092±0.805 pg/ml. As well as the case of TNF - ? which rose their concentration in serum of CL patients and reached to 98.145±2.925 pg/ml, then decrease after treatment 71.507±2.901 pg /ml, 48.381±2.379 pg/ml after 1 - 3dose and more of treatment respectively, while the mean of TNF - ? in control group was 13.173±1.388 pg/ml.An increase in the level of IL - 12 was observed in the group of CL patients 845.598±16.273 pg/ml then the level was dropped during treatment and recorded 619.164±20.209 pg/ml, 495.578±27.038 pg/ml respectively but in control the concentration was 160.222±11.761 pg/ml. IL - 17A level was elevated in CL patients 227.268±5.027 pg/ml and decreased during therapy reach to 170.512±5.481, 126.443 ± 5.565 pg/ml after 1 - 3 doses and four doses and more respectively. The concentration of MCP - 1 chemokine was increased in CL patients 111.0397± 6.985 pg/ml then dropped during treatment 71.084± 2.636 pg/ml after 1 - 3 doses then dropped more 49.543± 2.167 pg /ml after four doses and more while they were in control 12.268 ± 1.609 pg/ml. The results of statistical analysis showed significant differences between these cytokines and their groups at the level of probability less than 0.05.