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عزل وتشخيص اميبا Acanthamoeba castellani ودراسة تاثيرها الممرض في الفئران المختبرية Mus musculus سلالة Balb/C == Isolation and Detection of Acanthamoeba castellani. and study their pathological effect on laboratory mice Mus musculus Balb \C strain

Author name: شيماء عبد الرزاق حميد
Supervisor name: مسلم عبد الرحمن محمد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: free_living species of amoeba belongs to the genus Acanthamoeba was recorded in the recent study , this species was classified as A. castellani was according to the morphological features , cultural properties , heat tolerance and osmosis .Astromoeba was also recorded that characterized by its star - shape and Naegleria spp. which isolated from environmental samples ,both are free living amoeba .Acanthamoeba was isolated from different environments , includings water and soil from various regions of Basrah Provinace and from gathered water in air - cooler basin and discarded water from air conditioning systems .It was isolated from the eyes of out - patients who were suffered from eye problemes and from healthy volunteers.Several culture media were used for parasite culturing including media S, Agar, PYG, Mix , Mud , Heat - killed E.coli coated agar ,Lock and Manitol media.The media differ in their capability for the parasite growth depending on their nutrients ingrediants and the suitable temperature for the growth ,and the high rate for the growth was on S medium 37◦C and there is increasing in the parasite size that it is a new medium and used for the first time .The PYG medium at 37◦C also cause a good growth for the parasite .The parasite susceptibility to Dexonium was examined in vitro which shows the activity of the drug against the parasite .The activity of other drugs including Cisplatin , Pentostam , Chloromphenicol , Methadin , Cifran and Genedin was examined in this study .Cifran and Cisplatin shows a close effect and less than others ,while Methadin does not affect on the parasite ,in other hand Chloramphenicol caused obvious malformation in the parasite and retardation in the numbers.The recent study showed the death dose for half of the lab mice infected with the parasite was 450 amoeba/ml.The study also proved that the parasite is one of the major causes for Blinding ,causes it invades the mice eyes and blinds them moreover it cause skin lesions , hypertrophy in spleen and kidneys and bleeding in brain and lungs .Different ways used in the infecting of mice by the parasite caused the death of them in two - four dayes ,The chronic infection cause death in long period.The parasite can infect the brain when it is injected in different ways through optic nerve ,peritonium ,veins , nose and skin ,in which it caused necrosis .The presence of the trophozoite and cyst cause WBC infeltration.The liver ,kidneys and lungs were also infected when the parasite injected through the optic nerve , peritonium ,veins ,nose and skin and caused necrosis , bleeding patches , gathering of the defence cells (WBC).

تقدير بعض العوامل البيئية والبكتريولوجية لمياه الصرف الصحي في محافظة البصرة / العراق == Assessment of some environmental and bacteriological factors of Wastewater in Basra Province, Iraq

Author name: سهى محمد علي محمد سعيد المظفر
Supervisor name: منال محمد اكبر | ميثم ايوب عبد القادر
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية لمعرفة تاثير مياه الصرف الصحي على المحتوى البكتيري في محطة حمدان لمعالجة مياه الصرف الصحي /البصرة حيث تستلم المحطة مياه المجاري المنقولة عبر شبكة من الانابيب التي تستلم مياه المجاري من50% من مناطق مدينة البصرة. اختبرت ثلاث مواقع لاخذ العينات هي المحطة الاولى حوض التجميع الخارجي والمحطة الثانية حوض الترسيب الابتدائي اما المحطة الثالثة فهي المياه الخارجة من المحطة والتي تصب في شط البصرة.وشملت الدراسة ايضا قياس بعض الخصائص الفيزيائية والكيميائية لمياه المجاري وهي (الاس الهيدروجيني pH ودرجة الحرارة Temperature والملوحة Salinity والمتطلب الحيوي للاوكسجين BOD5 والفوسفات والمواد الصلبة العالقة الكلية TSS والمواد الصلبة الذائبة الكلية TDS والنتريت NO2 والنترات NO3) خلال الفترة من كانون الاول2014/ ولغاية ايار2015/.ويتضح من نتائج هذه الدراسة ان المتطلب الحيوي للاوكسجين BOD5 هو عامل مهم وله علاقة باعداد البكتريا الكلية الموجودة في مياه الصرف الصحي.كما شملت حساب كثافة المجاميع البكتيرية المتواجدة في مياه المحطات.ولحساب العدد الكلي للمستعمرات البكتيرية اخذت عينات مياه الصرف الصحي وزرعت على اوساط عامة وانتخابية وتفريقية مختلفة استخدم وسط الاكار المغذي Nutrient Agarكما واستخدم وسط المانيتول الملحي الانتخابي Mannitol Salt Agarلعزل المكورات العنقودية الذهبية Staphylococcusaureus ووسط اكار الماكونكي MacConkey Agar لعزل بكتريا القولون البرازية Escherichiacoli ووسط AgarXLD الانتخابي التفريقي (Xylose lysine deoxycholate Agar ) لعزل انواع البكتريا التابعة لجنس السالمونيلا Salmonella.تم ايضا دراسة حساسية العزلات تجاه المضادات الحيوية باستخدام طريقة الانتشار عبر الاقراص Disc Diffusion Test حسب طريقة كربي باور Kirby Bauer Method. ان دراسة حساسية عزلات E. coli توصلت الى ان العزلات البكتيرية ابدت نسب مقاومة عالية لاغلب المضادات الحيوية المختبرة في هذه الدراسة ، كذلك ابدت المكورات العنقودية الذهبية مقاومة عالية لاغلب المضادات الحيوية في حين كانت حساسة لكل من كلورامفينيكول (C) وسيبروفلوكساسين (CIP) ونورفلوكساسين (NOR) وكانامايسين (K). كانت عزلات السالمونيلا متباينة في حساسيتها تجاه المضادات الحيوية المختبرة في هذه الدراسة لكن ابدت عدم مقاومة او مقاومة واطئة للمضاد الحيوي CIP وNOR . ابدت المجاميع البكتيرية المختلفة قيد الدراسة انماط مقاومة مختلفة تجاه المضادات الحيوية اذ ابدت انماط مقاومة مختلفة. وكذلك ابدت العزلات البكتيرية المعزولة من مياه الصرف الصحي مستويات مختلفة من التحمل لتراكيز المعادن الثقيلة ،فيما يخص عزلات E. coliابدت العزلات مستوى عال من التحمل للنيكل بلغ 64% من العزلات كان MIC لها 4mg/Lوللكادميوم فان نسبة 93% من العزلات كان MICلها 0.8mg/L. اما بالنسبة للمكورات العنقودية الذهبية فان 89% من العزلات كان MIC لها 2mg/L لعنصر النيكلوبالنسبة للكادميوم فان 33% من العزلات كان MIC لها 0.4mg/L .وبالنسبة للسالمونيلا كان تحمل 100% من العزلات لعنصر النيكل هو عند 2mg/L في حين كان 50% من العزلات MIC لها 4mg/L كحد اقصى لعنصر الكادميوم. | The present study has been conducted to detect the effect of different parameters of sewage on microbial content at Hammdan for wastewater treatment in Basra city. The station receive sewage transported via net of pipes from different parts of Basra city .Three sites in the station for taking samples ,the first site is the external collection basin, the second site is primary sedimentation basin, the third site is water output that carry the water after treatment to flow at Shatt al Basra. Some characteristics of sewage were measured, these include pH, Temperature, Salinity, BOD5, TDS, TSS, Phosphate, NO2 and NO3 from period December/2014 to May/2015. In this study it is clear that BOD5 is an important factor related to total bacterial count in sewage. The bacteriological study of sewage has been done including measurement of total count of bacteria isolation and counting of Escherichia coli, Staphylococcus aureus and Salmonella spp. From different stations selective media is used for isolation including Nutrient Agar , MacConkey Agar , Mannitol salt Agar and XLD Agar . The antibiotic susceptibility of the isolates towards selected antibiotics have been studied using disc diffusion test. In the present study E. coli isolates showed high percentage of resistance to most of antibiotics. Staphylococcus aureus also showed high percentage resistance to most of antibiotics studied and found susceptibility to Chloramphenicol, Ciprofloxacin and Kanamycin. Variable suceptibility to tested antibiotics in the present study has been shown by Salmonella spp., Salmonella spp. were susceptible or show low resistance to Ciprofloxacin and Norfloxacin .Different Antibiotics Resistance Profiles has been shown by different bacteria groups studies. The bacterial isolates from sewage in this study have shown different levels of tolerance to different heavy metals (Nickel and Cadmium). E. coli tolerate high concentration for Nickel (%64) and for Cadmium (%93). High tolerance level for Cadmium has been shown for Salmonella spp (%50).

خصائص خمائر البيئة النهرية لمحافظة البصرة وقابلية بعضها على المعالجة الحيوية للعناصر الثقيلة والهيدروكربونات النفطية == Characterization of yeasts in river habitat in Basrah Province and an ability of some of them for Bioremediation of the heavy metals and oil hydro carbonate

Author name: سناء قاسم بدر
Supervisor name: باسل عبد الزهرة عباس
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:

تقييم حالة النباتات المائية الغاطسة في بعض مناطق هور شرق الحمار وشط العرب جنوبي العراق باستخدام بعض دلائل التنوع الاحيائي == Assessment of the Status of Submerged Aquatic Plants in Some Areas of East Hammar Marsh and Shatt Al - Arab River Southern Iraq Using Some Biodiversity Indices

Author name: سما عباس عبد المجيد الزيدي
Supervisor name: دنيا علي حسين العباوي
General topic: Biology
Specific topic: Aquatic Environment
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted to assess the state of submerged aquatic plants in some areas of East Hammar marsh and Shatt al - Arab River, as measured by some of the chemical and physical properties of water and sediment in six stations, three of which are located in East Hammar marsh (Nagara, Sallal and Almshab) and the other three are located in the Shatt al - Arab (the island Muhammadiyah, Salhiya and Mhella). Environmental surveys of submerged aquatic plants were done and registered their presence, their vegetation cover and biomass as well as the biodiversity indices (diversity and evenness, and dominance) for 12 months from October 2015 until September 2016.The air temperature in the East Hammar marsh ranged between 19 - 45.3 C, water temperature 14.3 - 32.4C , transparency between 20 - 100cm , Turbidity between 20.9 - 65.3 NTU, depth of 50 - 160 cm and dissolved oxygen between 5.4 - 6.9 mg / L, pH between 7.6 - 8.6 , electrical conductivity between 3.8 - 12.5 mS / cm, nitrates between102.23 - 549.64 μg/L, effective phosphorus between 4.15 - 9.6 μg/L . As for the Shatt al - Arab River values ranged : air temperature between 18 - 48 C , water temperature was between 14.2 - 32.3 , Transparency between 23 - 90 cm, turbidity between 12.8 - 68.3 NTU , depth of 60 - 200 cm , dissolved oxygen between 5.4 - 7.9 mg / L , pH between 7.5 - 8.51 , electrical conductivity between 2.8 - 12.4 mS / cm , nitrates between 106.17 - 569.42 μg/L, effective phosphorus between 4.93 - 9.13 μg /L .BpH and electrical conductivity values of the sediments in the Shatt al - Arab and East Hammar marsh were reached 7.7 - 8.3 and 1.08 - 2.91 mS / cm and 7.5 - 8.4 and 1.12 to 3.05 mS / cm respectively, effective phosphorus values in sediment ranged in sediment of East Hammar marsh between 5.69 - 42.51μg/L, While in Shatt al - Arab fluctuated between 5.12 - 40.3 μg/L, and the value of the organic matter in the Shatt al - Arab were 5.6 to 14.5%, while in the East Hammar marsh reached 7.4 - 15%. The sediments are similar texture in the Shatt al - Arab and East Hammar marsh (Silty clay).The presence of three species were recorded in the Shatt al - Arab River included Potamogeton crispus , Potamogeton pectinatus and Potamogeton perfoliatus, while five species in the East Hammar marsh Ceratophyllum demersum, Myriophyllun spicatum , Potamogeton crispus , Potamogeton pectinatus and Potamogeton perfoliatus.As calculated biodiversity evidence was that East Hammar marsh was more diverse than the Shatt al - Arab, while dominancy was clear in the Shatt al - Arab, compared with East Hammar marsh.This study showed that the sudden rise in salinity values as well as increased nutrients led to degradation of water quality, which was reflected on the presence and vegetation cover of submerged aquatic plants as well as biodiversity resulting from the presence of Tilapia and other fish that use submerged aquatic plants as food as well as the human factor.

تاثير هرمون الثايروكسين على بعض المعايير الوظيفية للدم وبعض الجينات المنظمة لتمايز النسيج العظمي في الفئران المختبريةmusculus L. Mus. == Effect of Thyroxine hormone on some of physiological parameters of blood and some regular genes of bone differentiation in Laboratory Mice Mus musculus L.

Author name: سلمى سعيد عباس
Supervisor name: سامي جبر المالكي | علي عبد اللطيف العلي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية لمعرفة تاثير هرمون الثايروكسين على تمايز العظم وبعض المعايير الفسلجية والتي شملت المعايير الدموية والهرمونات المحرضة للقند واوزان واطوال الاجنة واوزان المواليد في الفئران المختبرية البيضاء Mus musculus L.,اذ قسمت اناث الفئران المختبرية في الدراسة الحالية على مجموعتين هما : المجموعة الاولى الرئيسية : لدراسة تاثير هرمون الثايروكسين L.Thyroxine hormone على المعايير الدموية للاناث الحوامل وغير الحوامل وعلى تمايز العظم في الاجنة(باستخدام طريقة تحضير المقاطع النسجية الاعتيادية للاجنة والطريقة الجزيئية باستخلاص المادة الوراثية (RNA) من الاجنة وباستخدام تقنية النسخ العكسي RT - PCR والبلمرة الكمية qRT - PCR ومعرفة تاثيره على اوزان المواليد والاجنة, وتالفت من 90 انثى بالغة بعمر(10 - 12)اسبوع وقسمت على مجموعتين ثانوية ,و هي مجموعة السيطرة والتي حقنت حيواناتها بـ 0.1 مل من المحلول الفسيولوجي0.9% ولمدة 10 ايام متتالية.بينما المجموعة المعاملة حقنت حيواناتها بـ0.1 مل من هرمون الثايروكسين وبجرعة مقدارها 0.5 ملغم /كغم/يوم ولمدة 10 ايام يوميا بعدها شرحت 5 اناث من كلا المجموعتين ,و زوجت 40 انثى المتبقية ولكلا المجموعتين مع الاستمرار بالحقن لحين تشريح الاناث واستخراج الاجنة خلال فترات عمرية متباينة (9و10و11و12و13و14و15) يوم.اما المجموعة الثانية الرئيسية خصصت لدراسة تاثير هرمون الثايروكسين على الهرمونات المحرضة للقند وهرمونات الدرقية وانزيم Alkaline phosphatase.وتتالف من 15 انثى وقسمت على ثلاث مجاميع ثانوية تضم كل منها (5) اناث, وهذه المجاميع هي : مجموعة السيطرة وحقنت بـ0.1 مل من المحلول الفسيولوجي 0.9%.ومجموعة المعاملة الاولى وحقنت بـ0.1 مل من محلول هرمون الثايروكسين وبجرعة 0.5 ملغ/كغم/يوميا ولمدة 15 يوم للاناث العذارى قبل التزاوج.ومجموعة المعاملة الثانية حقنت بـ0.1 مل من محلول هرمون الثايروكسين وبجرعة 0.5 ملغ/كغم/يوميا ولمدة 30 يوم . اظهرت نتائج الدراسة الحالية ان المعايير الدموية قد تباينت قيمها خلال الفترات الزمنية وللمجموعات ضمن المدى الطبيعي عدا في حالات قليلة ,اذ وجد هناك انخفاض معنوي في اعداد خلايا الدم البيض والخلايا الحبيبية والخلايا الوحيدة والخلايا اللمفية والنسب المئوية للخلايا الحبيبية والوحيدة واللمفية واعداد كريات الدم الحمر,وكذلك قيم مستوى كمية الهيموكلوبينHb والنسب المئوية لحجم الدم المضغوط PCV(HCT)% ومعدل كمية الهيموكلوبين MCHومعدل تركيز الهيموكلوبينMCHC ومعدل حجم كريات الدم الحمرMCV والصفيحات الدموية PLT في فترات متباينة من الحمل للمجموعة المعاملة مقارنة مع مجموعة السيطرة. وبينت النتائج وجود زيادة معنوية في خلايا الدم البيض والخلايا الحبيبية والخلايا الوحيدة والخلايا اللمفية والنسب المئوية للخلايا الحبيبية والوحيدة واللمفية, ومعدل تركيز الهيموكلوبينMCHC والنسبة المئوية لحجم الدم المضغوط %HCT ومعدل حجم كريات الدم الحمر MCVو الصفيحات الدميةPLT في مدد متباينة من الحمل للمجموعة المعاملة مقارنة مع مجموعة السيطرة. وسجلت نتائج الدراسة الحالية حصول ارتفاع معنوي في قيم هرموني T4,T3في الحيوانات المعاملة مع انخفاض معنوي في تركيز هرمون TSHفي الاناث المعاملة مقارنة مع السيطرة,و اوضحت النتائج ايضا انخفاضا معنويا في مستوى الهرمون اللوتيني LH وهرمون الحليب وللمجموعتين(15و30) يوم وزيادة معنوية في الهرمون المحفز للجريبات FSHللمجموعة الثانية(30 يوما) ,مقارنة مع مجموعة السيطرة, ولايوجد تاثير معنوي لهرمون الثايروكسين على هرمون الشحمون الخصوي ولنفس الفترتين مقارنة مع مجموعة السيطرة, وحصلت زيادة معنوية في مستوى انزيم الفوسفاتيز القاعدي بعد 30 يوما من الحقن بهرمون الثايروكسين مقارنة مع مجموعة السيطرة ,وحصول انخفاض في اوزان الاجنة بعمر 15يوما والمواليد للاناث المعاملة مقارنة مع مجموعة السيطرة , وبينت الدراسة زيادة معنوية في اطوال الاجنة المعاملة بعمر 9 و10و11و15يوما مقارنة مع مجموعة السيطرة. اما الدراسة النسجية فقد بينت ان الهيكل العظمي في الاجنة بعمر 9و10و11 يوما عبارة عن بداءات اعضاء تتكون من نسيج ميزنكيمي,ولم تسجل الدراسة النسجية وجود فرق بين مجموعة السيطرة والمعاملة في تمايز العظم .وظهرت بداية التعظم الغشائي والغضروفي في الاجنة بعمر12يوما ,اذ لوحظ بداية تمايز الخلايا الميزنكيمية الى ارومات عظمية,مع وجود القليل من المادة البينية الشبيهة بالعظم بين الارومات العظمية في منطقة الفك السفلي قرب غضروف ميكل ,والفك العلوي والعظم الحنكي واللامي وغضاريف الاضلاع ,اما في الاجنة بعمر13 و14و15يوما فقد ازدادت عمليات تمايز العظم بنوعية ,فاضافة الى النمو الذي حصل في العظام السابقة في تكوينها فقد ظهرت عظام جديدة في مناطق اخرى من هيكل الجنين تمثلت بعظام الصفيحة القاعدية والفخذ وحزام الكتف وحزام الحوض والاضلاع ,اما النتائج الجزيئية الخاصة بتمايز النسيج العظمي في اجنة الفئران المختبرية فقد اشارت الى التعبير الجيني لبعض جينات النسيج العظمي وتشمل Runx2وOSX وOsteo(OSC) وSialo(BSP) والتي بلغت ذروتها في مرحلة (13) يوم. ان لهرمون الثايروكسين تاثير على التعبير الجيني لبعض الجينات التي ترتبط مع التمايز النسجي للعظم اذ يؤدي هذا الى التغير في التعبير الجيني زيادة احيانا وانخفاضا خلال المدد العمرية المختلفة للاجنة .نستنتج من الدراسة الحالية ان زيادة مستوى هرمون الثايروكسين يسبب اضطراب في المعايير الدموية والتعبير الجيني لجينات العظم وانخفاض مستوى الهرمونات المحرضة للقند (LH وProlactin) وزيادة مستوى الهرمون المحفز للجريبات, وتعد هذه الدراسة الاولى على مستوى جامعة البصرة | the present study was performed to know the effect of thyroxine hormone on differentiation of bone tissue and some of physiological parameters, which included blood parameters and Gonadotropine hormones and Weights and Lengths and birth weight of mus musculus L.the females are divided into two groups ;the main first group which included 90 adult females (10 - 12)week, that subjected to study effect of thyroxine hormone on blood parameters of pregnant , nonpregnant females ,and bone differentiation of embryos (by using tissue sections method and molecular technique by RT PCR , and qRT PCR), and also known it is effect on weight of fetus and newborns; this group was also subdivided into two secondary subgroup : - control group ; was injected with 0.1 ml of normal saline (0.9%) for 10 consecutive days ,and treated group; was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 10 consecutive days ,after that, 5 females from both groups were anatomized,while 40 females were mated of the both groups with continuous of injection until the females were subjected to anatomical practices and then the the embryo were taken during varied peroids (9,10,11,12,13,14,15) day. The main second group which included 15 females ,was selected to study the effect of thyroxine hormone on ;sexual hormone ,thyroid hormone and alkaline phosphatase . this group was divided into another 3 subgroups,one regarded as control group which ; was injected with 0.1 ml of normal saline (0.9%) for 15 consecutive days.and second the first treated group (T1) ; was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 15 consecutive days ,and without mating of the females,and the second treated group ; (T2);was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 30 consecutive days and also without mating of the females. Results of the present study were shown that the blood parameters were varied in value between periods and groups (decreasing and increasing among normal value ) with exception in few cases , significant decrease of W.B.C , the granular cells ,monocytes, lymphocytes ,lymphocytes percentage ,granulocytes percentage and monocytes percentage during different periods of pregnancy in the treated animals compared with control , and was decreased RBC ,Hb ,HCT percentage ,MCH ,MHCH ,MCV, and PLT in varied periods , and we noticed a significant increase in WBC ,granular cells ,monocytes ,lymphocytes , granulocytes percentage , monocytes percentage , MCHC ,HCT percentage,MCV, and PLT in varied periods of pregnancy in the treated animals compared with control. Our study was exhibited significant increase in levels of T3 and T4 hormones of the treated animals compared with control, with significant decrease in TSH of treated animals , also significant decrease in LH and prolactin H and for groups(15 and 30 days) ,and significant increasing in FSH of second group (30 day) ,when compared with the control , and there was no effect of thyroxine on TEST H level and for 2 periods(15 and 30) , significant increasing in alkaline pho after 30 days from injection with TH H , decreased of body weight of embryos (15 days) and newborns of the treated females ,the current study showed significant increase of lengths of treated embryos (9,10,11,15 days). The histological study was explained the skeletal tissue ossification of embryos during (9,10,11)day post gestation was primitive organs of mesenchymal tissue and The histological study was not show asignificant differences of bone differentiation between treated and control groups,and intramemberaneous ossification was appeared of embryos at 12 days ,in this stage the begining of differentiation of mesenchyme cells was appeared into osteoblast , with a little of matrix like bone between osteoblasts in Mandible near the meckle cartilage , Maxilla, Palatine,and Hyoid, and endochondral ossification as in Ribs, where in embryos (13,14,and 15) The bone differentiation processes were increased of bone (the intramembranous ossification and endochondral ossification),in addition to the growth of previously formed bones,also new bones were appeared in different areas in embryo skeleton like Basal plate,femur,Scapula girdle , pelvis girdle and the Ribs . In other hand, the molecular results of bone differentiation in embryo ,were exhibited peak of expression of some of the genes of bone tissue (Runx2 ,OSX, Osteo(OSC) ,and Sialo(BSP) ) at 13 days of embryo age, also the thyroxine hormone was effected in expression of some the bone genes that included disturbanceas like increasing of expression during peroids and decreasing in other peroids of embryos life. We concluded from the present study that the increase of thyroxine hormone level caused disturbances of blood parameters and gene expression of the bone genes & decreasing of the level of LH & prolactin hormones and increase the level of FSH. This is the first study on the Basrah University

تقييم فعالية عقاري Nandrolone decanuate وAlbendazole للاصابات المختلفة بداء الاكياس العدرية في الفئران المختبرية سلالة Balb/c == Evaluate the effectiveness of a drug Nandrolone decanuate and Albendazole for injuries the different infections by hydatid cysts in mice Balb/c

Author name: سعيد فاضل مناتي الحمداني
Supervisor name: مسلم عبد الرحمن محمد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: تبنت الدراسة الحالية استعمال طرق مختلفة لاحداث الاصابة برؤيسات الاولية Protoscolex المستخرجة من الطور اليرقي ( الاكياس العدرية ) لطفيلي Echinococcus granulosus وهذه الطرق هي الحقن الوريدي والجنبي وتحت الجلد وكذلك عن طريق الغشاء البريتوني . اظهرت الدراسة حدوث الاصابة وتطور الاكياس العدرية في كل الطرق اعلاه . كذلك تم استعمال طريقة الحقن في اللفائفي والتجريع عن طريق الفم لكن لم تظهر الاصابة المستخدمة بهذه الطرق اي تطور للاكياس العدرية في حيواتات التجربة . كذلك حقن 650 رؤيس اولي في جميع طرق الحقن وتبين حدوث الاصابة في نفس مواقع الحقن بطريقة الحقن تحت الجلد والحقن الجنبي وعن طريق الغشاء البريتوني. ولكن طريقة الحقن الوريدي اظهرت حدوث الاصابة وتطور الاكياس العدرية في اماكن مختلفة من جسم الحيوان المختبري ويكون الكيس العدري متموضعا بين نسيج العضو المصاب في حين الحقن عن طريق الجنب وتحت الجلد والغشاء البريتوني يكون الاكياس العدرية المتطورة ملتصقة على سطح العضو المصاب . كذلك اثبتت الدراسة وجود اختلاف في الوان الاكياس العدرية المتكونه وحسب طريقة الحقن. استـــــعمل في هذه الدراســــــــة عقار Albendazole وعــــــقار Nandrolone decanuate كعقارات علاجات للاصابة المستعملة وتبين ان لها تاثير ادى الى موت الرؤيسات الاولية بتقدم فترة الاصابة واثبتت الدراسة ان عقار Nandrolone اكثر كفاءة من عقار البندازول وذلك من خلال الاختلافات في النسبة المئوية لموت الرؤيسات الاولية وكذلك بسبب هذا العقارشوه في شكل الرؤيسات وطريقة ابعاجها اذ كان الانبعاج الى الخارج في حين كان الانبعاج الى الداخل في الاصابات المعالجة بعقار البندازول . | The present study showed various methods for the infection by the protoscolex obtained from the larval phase of the Echinococcus granulosus . which involved the venus,pleural,subcutaneous and peritoneal injection . In all injection method 650 protoscolex were injected ,It was found that no infection happen in the same location to the injection which resulted by the subcutaneous,pleural and pretonal injections. On the contrary to thatresulted by venous injection in wich the injection occurred in varoes sites of the lab animals body ,and the hydatid cysts were pervasine with in the diseased infected organ tissues. Wile that resulted by pertonial ,subcutaneous and hered the surface of the organ. The colors of the hydrated cysts were also varied according to the infection methods.Albendazole and Nandrolone were used as treatments against the induced infections and it was found that Nandrolone was more efficient than albendazole,That was observed by the rates of the protoscolex caused by the action of the drugs and desmorphorphology and invagination outward while in albendazole was inward

دراسة مقارنة لتاثير هرمون النمو في عضلات وCyprinus مناسل سمكتي الكارب الاعتيادي والبلطي احمر البطن carpio (L. 1758) Tilapia zillii (Gervais, 1848 == A Comparative Study to the Effect of Growth Hormone on Muscles and Gonads of Carp Cyprinus carpio (L. 1758) and Tilapia Tilapia zillii (Gervais, 1848)

Author name: سعد محمد صالح عبد الصمد
Supervisor name: عقيل جميل منصور | عبد الكريم طاهر يسر
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: In the present study, the effect of human growth hormone on the muscles and gonads of common carp Cyprinus carpio (L. 1758) and red belly tilapia Tilapia zillii (Gervais, 1848) was studied by : - 1 - Studying the effect of human growth hormone on the growth of the two studied species by intraperitoneal (IP) injection of three replicates of control with 120 μg of normal saline / g Body weight and two doses (low and high) with 60 and 120 μg of human growth hormone / g Body weight respectively for both species, then changes were followed up in each treatment after every two weeks of injection by : - a - Pursue histological changes in the muscular tissue by using Cell Profiler software for the first time in Iraq. b - Pursue changes in chemical components ratios of body muscles.c - Measuring of specific growth rate and length - weight relationship for fishes.2 - Studying of human growth hormone effect on the gonads of fishes (adult males and females) with 60 males for each species and a same number of females, all of them injected either with low or high dose with 60 or 120 μg of human growth hormone / g Body weight respectively for both species or with 120 μg of normal saline / g Body weight in fishes of control. This experiment had achieved in a month.3 - Knowing the direct effect of human growth hormone on the follicles by implementing an in vitro experiment with the follicles of carp which implanted in basic salt solution (BBS) then growth hormone was added to the test tubes with two concentrations 5 μg/ ml and 10 μg/ ml, the control follicles were implanted in normal saline. The results of the study revealed that the maximum rate of percentages of the muscle fibers with area less than 314 μm2 for region R1(Dorsal side) was 88.29 % in carp in high dose treatment after the eighth week of injection, while it was 88.00 % for tilapia in high dose treatment after the eighth week of injection, too.In the region R2 (in tail) the maximum rate of percentages of muscle fibers with area less than 314 μm2 was recorded after the eighth week from the beginning of injection was 73.3553 % for carp in high dose treatment and 85.935% for tilapia in high dose treatment.The results showed that 1.00984 and 1.000074 were the highest and the lowest values of condition factor for carp respectively. For tilapia those were 1.009993 and 1.000151 the highest and the lowest values of it respectively.The rate of specific growth rate for length was 0.3616 and 0.44866 cm/day% for carp and tilapia respectively. While the specific growth rates for weight were higher than the comparable one for length and were 1.3489 g/day% for carp and 1.6085 g/day% for tilapia.The results exhibited that the rates of protein percentages for both species in the all treatments in general tend to be higher with progressing of experimental time. Muscle water content percentage rate varied with difference of dose and experimental time. The maximum rate of fat percentage in both species was in control treatment after two weeks of hormone administration.The highest rate of carp male Gonadosomatic index was 6.55% in high dose treatment after the third week of injection, as for tilapia males was 6.103% in low dose treatment after the third week of injection, while the lowest value for this index was recorded after the first week of injection in high dose treatment which as 2.81 and 2.89% for carp and tilapia respectively. For females the highest rate of Gonadosomatic index was in high dose treatment after the third week of injection for both species which was 11.001 and 6.0624% for carp and tilapia respectively. As for the lowest rate of carp female Gonadosomatic index was recorded in control treatment after the first week of injection it was 5.54%, while the lowest rate of this index for tilapia females was 2.99% in low dose treatment after the first week of injection.In the experiment of direct effect of this hormone on the follicles of carp it was found that the highest value of polarization index was 4.12 which recorded in high dose treatment after 48 hours of incubation while it reached the lowest value which was 3.031 in high dose treatment after 24 hours ofincubation.

دراسة تشخيصية مظهرية وجزيئية للشريطيات المتطفلة على نورس مستدق المنقار Larus genei والكرسوع Himantopus himantopus شمال محافظة البصرة == Diagnostic, Morphological and Molecular Studies on some cestodes parasitized on Larus genei and Himantopus himantopus , North of Basrah province

Author name: سحر محمد علي محمد سعيد المظفر
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: During the period from August 2015 to July 2016, fifty three aquatic birds of Larus genei (slender - billed Gull) family Laridae and 52 of Himantopus himantopus family Recurvirostridae were collected from Al - masehab marsh north of Basrah Province.The current study was focused on these two birds due to its economic importance to the local people of the study area. These two birds were found infected with 10 species of cestodes. Echinocotyle longirostris and Infula burhini were recorded for the first time in Iraq. Larus genei was considered as a new host of Wardium fusa.The isolated cestodes were described and compared with other recorded species at different parts of Iraq and the world.The percentage and the mean intensity of infection of these 10 species of cestodes were counted monthly and seasonly together with single, double and trible infection during the whole year. The main percentage infection was reached to 95.2% and the main intensity was 13.49. In L. genei the percentage and mean intensity were 90.5% and 15.4 recepectively, while for H. himantopus were 100% and 11.8 recpectively. In L. genei the high percentage infection was found in August 2015 and September , November, and December 2016, and during March, April, May, June and July for H. himantopus. A significant difference was recorded between the percentage and the mean intensity of infection.Random Amplified Polymorphic DNA (RAPD) technique with random 6 primers was used to distinguish between Infula burhini and Acoleus vaginatus wich isolated from the bird H. himantopus due to the morphological similarities.The Scanning Electron Microscope (Pharmacy College, Basrah University) was used to photograph the scolex of A. vaginatus and I. burhini.

تشخيص وحياتية حشرة مع تصنيف الديدان الخيطية المتطفلة عليهافي محافظة ميسان == Diagnosis and life cycle insect Gryllotalpa gryllotalpa (L.) with a taxonomy parasitic nematodes in the province of Missan

Author name: سجى عماد نعيم الساعدي
Supervisor name: باسم هاشم عبد الله | ضياء خليف كريم
General topic: Biology
Specific topic: Zoology
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: It was collected 318 mole cricket insects randomly from four regions in Missan including AL Kahala district by 191 insects, AL Mishraah by 62 insects, Kalat Salih by 61 insects and New Hay AL Hussein ( Centre of the city) by four insects. The period was from September 2013 to August 2014. It was diagnosed one species of mole crickets which is G. gryllotalpa decended from Gryllotalpidae families because of its essential charact ristics such as the general form of the insect, shape of the wings and legs, and its site and colour. In addition, there is a large lobe in the front legs of the insect and there one two species of veins in the front wings of the male used to attract females for mating. The life cycle of the insect was studied in the laboratory. Itstransformation is characterized by being imperfect progress, on egg, then a nymph and then an adult. The female lays about 100 eggs oval - shaped and grey in a room in the soil. The period of preegg - laying is approximately 100 days and the percentage of hatchling is 74% . The females remain alive after one day egg - laying is completed while the males remain 13 days after egg - laying. The eggs hatch into nymphs which pass stages of life development andit takes 115 day - Newly hatched nymphs are transparent and grey, its length is 10 mm with antennas and small cerci which are without wings. Wings begin to appear in the sixth stage of life. Then nymphs develop into brown adults with a large filled body. Its length is about 40 mm and all parts of the insect continues to grow in proportion to the size of the insect.After examining these insect, it showed that they are infected with seven species of nematode parasites. They are Binema ornate, Binema korsakowi, Binema anulinervus, Cameronia triovata, Cameronia multiovata, Mirzaiella asiatica, Grylophilla skrjabini. The Prevalence of those nematodes are 18%, 13%, 1%, 2%, 1.0%, 22% and 25.6% respectively. while the mean intensity of infection was 1.5, 1.6, 3.5, 4.5, 8, 1.5,2.15 respectively.Four of these species of nematodes were considered as new record in Iraq, These are : Binema anulinervus : Most important feature in the shape of the high - tail and long that look like a flagella.Mirzaiella asiatica : Featuring male this form of the parasite by the short and broad and meandering tail. The female is characterized by a length of the muscle of the esophagus and the existence of the tapes curled at his side. Cameronia triovata : The most important feature of this parasite egg shaped oval that is flat from one side. C. multiovata : What also distinguishes this form of the parasite is a large and long oval egg.Nematodes were a taxonomically quoited and illustrated.The species B. ornate and G. skrjabini appear repeatedly over 10 months followed by the parasite B. korsakowi for eight months then followed by M. asiatica for seven months, as for the parasites B. anulinervus, C. triovata, C. multiovata for two months

دراسة مظهرية وجزيئية بتقنية RAPD لبعض الاصناف الغيبانية لنخيل التمر في البصرة == Morphological and Molecular Study by RAPD Technique for Some Un Known (Ghaibany)Cultivars date - palme In Basrah

Author name: زينب شعلان عبد الرزاق علي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية اثناء الموسم الزراعي للعام 2015م على احد عشر صنفا بذريا (غيباني) لنخيل التمر Phoenix dactylifera L. النامية في البصرة وهي اصناف جديدة تسجل لاول مرة, درست ثمان وعشرون صفة من صفات النمو الخضري, وست وعشرون صفة من الصفات الثمرية كما. درست صفات البذور, فتبين ان الاصناف المدروسة اختلفت في الخواص الخضرية والثمرية, اذ اظهرت الدراسة ان كلا الصنفين نبوي والصنف نجمة تميزا بشكل التاج الشبه منفتح في حين كانت الاصناف الباقية مضمومة التاج. وكذلك انفرد الصنف نجمة عن باقي الاصناف بلون الاوراق الاخضر الشمعي. وتراوح معدل طول الورقة من 2.68 م في صنف الوردية الى 4.35م في الصنف نمروشة, كما تراوح معدل طول منطقة الاشواك من 56 سم في صنف دكلة عبد الى 160سم في الصنف ام اللسين. وتباينت الاصناف في معدل عدد الاشواك فكان ما بين 18 شوكة في الصنف وردية الى 47 شوكة في الصنف ام اللسين. واختلفت الاصناف بنمط ترتيب الاشواك على المحور الوسطي فقد انفرد الصنف برماني بنمط توزيع الاشواك الثنائي وامتاز الصنف وردية بالنمط المفرد الثنائي في حين كان النمط المفرد هو السائد في الاصناف الباقية, واتضح من الدراسة ان النسبة المئوية لطول منطقة الاشواك الى طول الورقة الكلي هي من الصفات التي تتسم بالثبات دونا عن باقي الصفات الخضرية. اما بالنسبة للخواص الثمرية فقد تراوح معدل طول العرجون للاصناف المدروسة مابين 66 سم في الصنف رحماني الى 152سم في الصنف برماني والذي يعد من العراجين الطويلة, وانفرد الصنف دكلة داود بلون العرجون الاصفر المائل للوردي اما الصنفين برماني ونجمة فقد كان لون العراجين فيهما اخضر في حين كان لون العراجين في الصنفين وردية ونبوي اصفر يميل للاخضرار واصفر في باقي الاصناف. اما الخواص الفيزيائية المدروسة للثمار فقد سجل الصنف دكل داود اقل المعدلات في حجم الثمرة في مرحلتي الخلال والتمر والتي كانت 5سم3 و2.6سم3 على التوالي, وامتاز الصنف الزريجي بكبر حجم ثماره لكلا المرحلتين الخلال والتمر 17سم3 و10.24سم3 على التوالي, وتبين ان طعم الخلال في الاصناف وردية ودكلة عبد والصنف رحماني والصنف زريجي عفصي اما الاصناف الباقية فهي حلوة المذاق. وامتاز الصنف نجمة بكونه مبكر جدا في موعد النضج وكانت الاصناف الباقية مبكرة النضج في حين كان الصنف برماني متوسط النضج, وامتازت اصناف الدراسة بكون التمور فيها طرية القوام عدا الصنف رحماني الذي كان قوام التمر فيه شبه جاف. واستخدمت الدراسة برنامج التحليل الالي لتحليل المكونات الرئيسة للثمار في مرحلة الخلال, فتبين ان الاصناف انقسمت بمجموعتين وحسب التقارب في البيانات المغذاة للبرنامج فانعزل الصنف الزريجي بمجموعة مستقلة لكون بياناته متفردة, اما المجموعة الثانية فقد توزعت الاصناف فيها الى تحت مجموعة, صغرى ضمت الاصناف دكلة داود ونجمة واقترب منهما الصنف وردية, اما الاصناف الباقية فتوزعت هي الاخرى بتحت مجموعتين. استخدمت الدراسة الجزيئية تقانة PCR RAPD لتثبيت البصمة الوراثية للاصناف المدروسة ولكشف الارتباط الوراثي بينها, وجرى باختبار عشرة بادئات عشوائية من مجموعة OPA وبادئان من مجموعة OPB. تم تحليل نواتج التفاعلات للبادئات مع Genomic DNA الاصناف كافة وكل على حده لتحديد مدى كفات البادئ فكانت النتيجة ان اربعة من البادئات ( OPA0.2 وOPA0.3 وOPA0.5 وOPA0.6) نجحت في كشف التباين بين الاصناف المدروسة, وبينت نتائج التحليل العنقودي للنواتج التفاعلات للبادئات ان الاصناف توزعت بمجموعتين, صغرى ضمت كل من الاصناف زريجي ونمروشة والصنف ام اللسين ومجموعة كبرى ضمت الاصناف الباقية, وقد توزعت فيها الاصناف الى تحت مجموعة ثانوية. مما يشير الى الاصول المشتركة, وبينت الدراسة امكانية انتخاب اصناف بذرية تمتاز بخصائص ثمرية جيدة وتكثيرها لدعم الاقتصاد الوطني. | This study was performed during agronomic season in 2015 on 11 cultivars (ghaibany) Phoenix dactylifera L. that grow in basrah, and these cultivars are new recorder. Twenty eight characteristics of vegetative growth were studied, seed characteristics were studied also. It was concluded that the studied cultivars varied in there vegetative and fruit qualities. The study showed that both cv Nabawy and cv Najma distinguished by semi - opened crown but the other cultivars had closed crown . In addition, cv Najma marked by waxen green leaves. The rate of leaf length ranged from 2.68 m in. cv Wardiah to 4.35m in cv Nammrosha. besides the length of spine area ranged from 56 cm in cv Deglat Abd cultivars to 160 cm in cv Am - Ellsian. The cultivars also varied in the rate of spine number which was 18 in cv Wardiah an 47 in cv Am - Ellsian. The cultivars differed in the style of spine arrangement on rachis since cv Bermany distinguished by the dual style of spine arrangement, while in cv Wardiah marked by single dual style, however the single style was prevalent in other cultivars. The study clarified that the percentage between spine area to the total length of the leaf was one of qualities that characterized by stability that differed from other vegetative characteristics. As for fruit characteristics, the length of peduncle of cultivars concerned ranged from 66 in cv Rahmany cultivars to 152 in Bermany and that was regarded long peduncle. cv Deglat Dawood marked by colour of peduncle which was yellow look like rosy, whereas cv Bermany and cv Najma the colour of their peduncle were yellow look like green and yellow in the rest. As for physical characteristics of studied fruit, cv Deglat Dawood recorded the lowest rates in the volume of fruit during the phases of khalal and dates, the volume was 5 c.m and 2.6 c.m respectively, cv Zraigai distinguished by the bigness of the volume of the fruit for both phases of khalal and date 17c.m and 10.24 c.m. The taste of khalal in the cultivars of cv Deglat Abd, cv Wardiah, Rahmany, cv Zreigai was tannic while the taste of other cultivars were sweet. cv Najma cultivars distinguished by being so early in maturity, the other cultivars were early in maturity but cv Bermany was middle in maturity. The dates of studied cultivars were soft except cv Rahmany that was semi - dry. The study used a program of automatic analysis to analyze the main components of fruit in khalal phases the, it became clear that the cultivars divided into two groups according to the convergence data that fed to the program. cv Zraigai cultivars was isolated in independent group because its data were unique, but the second group ,cultivars in which were classified in small group included cultivars of cv Deglat Dawood and cv Najma and cv Wardiah approached to this group. The other cultivars classified into two groups.The molecular study used P C R RAPD technique to fix genetic print of studied cultivars and to discover the genetic link among them. Ten of random primers of OPA group were tested and two of OPB were tested too. The products of reaction of primers were analyzed Genomic DNA for cultivars totally and individually; to determine the efficiency of primer the results was that for of primers succeeded in discovering the variation among cultivars concerned. The result of cluster analysis of reaction products of primers showed that the cultivars divided into two groups : small group included cv Zairgai, cv Nammrosha and cv Am - Ellsian cultivars, and big group contained the other cultivars in which cultivars distributed into secondary group and that refer to the common origin. The study showed the ability of electing and increasing Ghaibany cultivars that marked by good characteristics to support national economy

دراسة تصنيفية وحياتية للفطريات الناقصة الملونة في البصرة == Taxonomical and Biological study of Dematiaceous imperfect - fungi in Basrah

Author name: زينب خلف عبد الله
Supervisor name: توفيق محمد محسن
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: During this study 23 species belong to 10 genera of imperfect fungi were isolated from different natural sources ( plants, soil , and ari) from Basrah govermanete using different isolation methods.Among the isolated fungi the genus Alternaria was selected to identify its species by using the morphological characteristis by light microscopy and Scaning Electron in Microscope (SEM) and verified by molecular and chemical methods. Eight species of Alternaria were identified namely; A.alternata (isolate1 and 2) A.chlamydospora A.citri, A.raphani , A.longipes , A.infectoria A.tenuissima and A.chrysanthemi based on light microscope examination.Taxonomic relegation of these species were confirmed by SEM image. SEM revealed that there was differences in the conidial wall ornamentation .The molecular analysis using PCR depending on the loci ITS1, and ITS4 700bp which confirmed the morphological identification of most of species namely A.alternata (isolate 1 and 2) , A.chlamydospora , A.longipes , A.infectroia and A.tenuissima. However , A.citri was related to A.alternata, A.raphani was belonged to A.infectoria and A.chrysanthemi is belonging to A.lolii. Nevertheless ,by using the loci OPA1 - 3 there was no variation among the species of Alternaria ,this is may be due to the inadequate method applied.The genetic variation of 36 isolates of A.alternata was studied using 9 - primers by RAPD - PCR technique. All primes gave an amplification of DNA for all the fungal isolates. Among the primers OPC08 exhibited a highest efficacy reaching 235 total bands .In the present study the antibacterial activity of fungal crude extract was tested. It appeared that the extract exhibit an antibacterial bioactivity against E.coli , S.aureus , S.epidermidis and S.saprophyticus.However, the extract of A.alternata (isolate 1 and 2) showed an inhibition growth against all the tested bacterial strains. This study was also revealed that the fungal filtrates of Alternaria species possessed various chemical compounds. However, there were differences in the chemical composition among the fungal filtrates.The results showed that Alternaria species ( A.alternata isolate 1 and 2, A.chlamydospora A.citri, A.raphani , A.longipes) were capable to biosynthesize silver nanoparticles, when the fungal filtrate were treated with AgNO3 solution after72 hrs incubation .Silver nanoparticles synthesis was checked by the change of colour from yellow to brown and confirmed by Uv - vis spectrophotometry at wave length (420, 440 nm). SEM image showed that the silver nanoparticles with spherical shape and size ranged between (20 - 72nm). Aconclusion can be derived that application of SEM,chemical analysis of fungi extracts ,molecular analysis and the silver nanoparticles characteristics are useful in the taxonomy of fungi to solve the overlapping of the morphological identification .Taxonomic key were prposed for the species of the genes Alternaria based on the morphology , chemical compound with the fungal extracts and size of silver nanoparticles synthesized by fungi .Melanin pigment identify by TEM microscope and it appeare like dark drop inside the cell .

دراسة المايكوبلازما المصاحبة لحالات العقم في الرجال في محافظة البصرة == Study of Mycoplasma associated with men infertility in Basrah province

Author name: زهراء كاتب جمعة
Supervisor name: غيداء جاسم عبد النبي الغزاوي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: This study aimed current was to determine the role of Mycoplasma in men infertility in Basrah province . A100 seminal fluid specimens were collected from men who were admitted to infertility center in Basrah province from 1/10/2015 to 31/5/2016 .Besides 50 semen specimens were collected from those who determine fertility men as control group. Samples then were cultured on Monophasic - Diphasic - Culture - Setup(MDCS) to isolation Mycoplasma then the samples were cultured on Blood agar, MacConkey agar and Chocolate agar to isolation bacteria other than Mycoplasma .The isolated Mycoplasma from 48 cases were concluded five species : Ureaplasma urealyticum 38% ; Mycoplasma hominis 21%;Mycoplasma genitalium 15% ; Mycoplasma pirum 15% and Mycoplasma fermentans 11% . Where the last two species are first recorded in Iraq from semen.This Study also recorded that the patients of 30 - 39 years old were of high infertility rate and Mycoplasma infection percentage.High the percentage of a single infection was related to U. urealyticum and Mycoplasma hominis .By using the double Vitek 2 system 22 other bacterial species were diagnosed from seminal fluid. some of these species were first recorded in Iraq and represented types next : Staphylococcus haemolyticus ,,Enterococcus faecalis Staphylococcus hominis , Staphylococcus warneri ,Kocuria rosea , Streptococcus pneumoniae , Kocuria kristinae ,Granulicatella elegans , Vibrio cholera , Aeromonas salmonicida ,Enterococcus faecium , Morganella morganii, Enterobacter cloacae ,Alcaligenes faecalis , Staphylococcus xylosus , Micrococcus lutes ,Granulicatella adiacens, Kocuria varians,And the most exhibited and isolated bacterial species was of Staphylococcus haemolyticus.Mycoplasma infection were noticed in patients who were suffering from other diseases like varicose 18.75% and diabetes 8.33% and bladder nerve pressure 4.16% .In this study the primary infertility was 82% included 39 cases infected with Mycoplasma , while for the secondary infertility 18% nine cases were infected with Mycoplasma .High infection rate of Mycoplasma 69.23% was recorded in patients who underwent a varicose other Surgery compare with the other patients.Mycoplasma infection for the five species was clear quantity and quality in semen analysis for infertile men was less than control group .Where the semen volume was very small and the sperms count was effect on also the sperm morphology and activity (mobility) were affected by Mycoplasma infection . Pus cells and RBC. Were appeared in semen.This study also , confirmed that there is a 7% of semen from infertile men , was free from bacterial infection.

عزل وتشخيص بعض انواع من بكتريا المايكوبلازما من عينات سريرية في مدينة البصرة == Isolation and identification of some Mycoplasma species from clinical samples in Basrah city

Author name: رواء صادق مجيد
Supervisor name: غيداء جاسم عبد النبي الغزاوي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: In the present study , a total of 150 clinical samples were collected (50 samples of sputum ,50 swabs of gingiva and 50 vaginal swabs ) from patients visited Basrah general hospital and from the 1st specialized center of dental medicine in the city of Basrah . This study extended from January 2015 to May 2015. Samples were taken from males and females (except vaginal swabs where were collected from females only) .Their ages ranged from 6 - 70 years old. These samples were collected and cultured in a method monophasic - diphasic culture setup ( MDCS ) . Three types of Mycoplasma were isolated : Mycoplasma pneumoniae from sputum , Mycoplasma salivarium from gingival swabs and Ureaplasma urealyticum from vaginal swabs .These isolates diagnosed by biochemical tests and PCR. Mycoplasma spp. were isolated from 76 individuals out of 150 enrolled in this study, infection rate was 50.5% , 25 cases were diagnosed as M. pneumoniae (50%) , 13 cases were diagnosed as M. salivarium (26%) and 38 cases were diagnosed as U. urealyticum (76%).Mycoplasma was studied in related with two factors : sex and age . In this study, the Mycoplasma medium is modified , egg yolk was used instead of horse serum as source of cholesterol and also used two amino acids Arginine and Cysteine as well as urea ( when diagnosing U. urealyticum ) to support Mycoplasma growth. Besides that , thallium acetate was replaced by sodium benzoate , finally , magnesium sulfate was used as a sign of ammonia when diagnosing U. urealyticum.In this study , PCR technique used to diagnose species of Mycoplasma using ATPase gene ,16s rRNA gene and Urease gene .Sixty - eight isolates were identified by PCR technique , 25 of them were M. pneumoniae , 13 were M. salivarium and 32 were U. urealyticum.

دراسة مجتمعات اللافقريات القاعية الكبيرة في ثلاث بيئات مائية مختلفة في جنوب العراق == Synecology of Macrobenthic Invertebrates of Three Different Aquatic Habitat at Southern Iraq

Author name: رغد زيدان خلف
Supervisor name: هيفاء علي حمزة | مرتضى يوسف العباد
General topic: Biology
Specific topic: Zoology - Invertebrates
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study aimed macrobenthic invertebrats communites in three different aquatic environments southern Iraq. Eight stations were chosen for this purpose, Four of them distinction by being with running water and affected with the tide of Arabian Gulf,these include two stations on each of Shatt Al - Arab River (Al - Mohamadiat and Al - Salihia) and east part of Al - Hammar marsh (Al - Burgah and multaqaa Al - Mashab and Al - Sallal).the other four stations were not affected with the tide and located on the central marshes, two stations on each of Aum Al - Showaich marsh (Al - Sibitia and Al - Badria) and Al - Chebaeish marsh (Abu Sobat and Al - Sihaqee).The samples of water and sediment were collected monthly during the period from April 2014 to March 2015.Some environmental factors for each station were measured these include air and water temperatures, pH ,electrical conductivity, turbidity, dissolved oxygen, biological oxygen demand, total dissolved soild, total hardness, Calcium, nitrate , reactive phosphate, sulphate, total organic carbon and soil texture. The macrobenthic invertebrates samples which collected by Three replicates monthly for each station were isolated and identified,then densities and relative abundance were calculated.Some ecological indices such as diversity, richness, evenness, dominance, beta diversity and similarity indices(qualitative and numerical) were studied. Canonical correspondence analysis was adopted to evaluate the effect of the measured environmental factors on the occurrence and density of the studied macrobenthic invertebrates.The results showed that the variables of the stations were ranged as below : Air temperature (12 - 44)C̊ ,while the water temperatures (10.6 - 35)C̊ , PH (7.5 - 9.1), EC (2.07 - 13.7) mS /cm, Turbidity (4.2 - 199) NTU, DO (5 - 11.5) mg/l , BOD5 (0.3 - 3.5)mg/l , TDS(1245 - 10220)mg/l , TH (531 - 2928) mg/l , Ca+2 (80.2 - 320)mg/l , NO3 - (0.26 - 15.25) mg/l , PO4 - 3 (0.032 - 2.9) mg/l , SO4 - 2 (250 - 910)mg/l , and for sediments : TOC (9.3 - 22.5)% and soil texture ranged between sandy silt to muddy silt.The total number of recorded taxes were 80,representing by 16 species belonging to Annalida, 36 genus to the Aquatic insects (28 genus of them were first record in the middle wetlands), 16 species to Crustacea and 12 species to Mullosca. The species Pomacea canaliculata was recorded for the first time from Shatt Al - Arab river. The recorded taxes were classified according to its occurrence periods into four classes : dominant, fluctuated, less abundant and rare.The monthly density of each taxa of macrobenthic invertebrate and for each main macrobenthic invertebrate group in all stations was calculated. The results revealed that the densities were ranged from( 0 - 7115, 0 - 485 , 0 - 703 and 0 - 1199) ind/m2 for the groups of annelid, aquatic insect, crustacean and mollusca respectively. While the total density of all macrobenthic invertebrate groups was ranged between( 0 - 7367) ind/m2.The results of annual relative abundance for each main macrobenthic invertebrate group in all study stations referred that the value 66.3% was recorded for the annelids, while the lowest one 6.1% was recorded for the aquatic insects. On the other hand, the annual relative abundance for each main group in each station was investigated, and was ranged between (0.9 - 68)% recorded in Shatt Al - Arab river stations for the groups of aquatic insects and annelids respectively,( 0.4 - 67)% recorded in east Hammar marsh stations for groups of water insects and mollusks respectively, and (1 - 82)% recorded in Middle marshes stations for the annelids. The annual relative abundance for total macrobenthic invertebrate group in all stations was ranged from (2 - 27)% recorded in Al - Sibitia and Al - Sihaqee stations respectively. The annual relative abundance for the species and genus which recorded during our study revealedthat all highest values (94, 96.58 and 100)% were recorded for the species Limnodrilus hoffmeisteri in stations of Shatt Al - Arab River, east Hammar marsh, and Middle marshes respectively.The monthly relative abundance for main macrobenthic invertebrate groups in all station was studied. The highest value which recorded from Shatt Al - Arab river stations (Al - Salihia station) was reached 95.8% and recorded for the annelids, while reached 100% either in east Hammar marsh stations(Al - Burgah) for the mollusks and in Middle marshes stations (Al - Sibitia) for the crustaceans.Some ecological indices for each for each main macrobenthic invertebrate group from all stations totally were calculated. About the spatial diversity the range of Shannon - Wiener diversity index were between 0 - 0.6674 for annelids, 0.8718 - 1.92 for aquatic insects,0.5404 - 1.917 for crustaceans and 0.3035 - 1.15 for mollusks, while were 1.076 - 2.085 for all the benthic groups totally.The values of Richness index were from 0 - 1.127 for annelids,0.778 - 3.683 for aquatic insects, 0.5212 - 2.104 for crustaceans and 0.2543 - 1.277 for mollusks, while were 2.321 - 5.225 for all the benthic groups totally. The values of Evenness index were ranged between 0 - 0.9629 for annelids,0.4649 - 0.7419 for aquatic insects ,0.3016 - 0.8327 for crustaceans, and were 0.2189 - 0.8974 for mollusks, while were 0.3121 - 0.77 for all benthic groups totally. The values of Dominance index were ranged from 0.6129 - 1 for annelids, 0.343 - 0.7736 for aquatic insects ,0.2991 - 0.8772 for crustaceans,0.5075 - 0.9845 for mollusks while were 0.2638 - 0.7762 for all benthic groups totally.About the temporal diversity the values of Shannon - Wiener diversity index were between 0 - 0.99 for annelids, 0 - 2.01 for aquatic insects, 0 - 1.5 for crustaceans and 0 - 1.637 for mollusks, while were 0 - 1.981 for all benthic groups totally. The values of Richness index were from 0 - 0.969 for annelids, 0 - 2.717 for aquatic insects, 0 - 1.314 for crustaceans and 0 - 731 for mollusks, whilewere 0 - 3.047 for all the benthic groups totally. The values of Evenness index were ranged between 0 - 1 for each of annelids, aquatic insects and crustaceans, and were 0 - 0.992 for mollusks, while were 0 - 971 for all benthic groups totally. The values of Dominance index were ranged from 0 - 1 for each of four benthic groups, while were 0.3 - 1 for all benthic groups totally.Whittaker Beta index between the three different aquatic habitats for each main macrobenthic invertebrate group was calculated. The lowest value 1.14 was recorded for the mollusks between the middle marsh of um Al - Shoowaich and Al - Chebaeish, and highest value 1.66 was recorded for the aquatic insects between the east Hammar marsh and marsh of Al - Chebaeish, while the index values for all groups of macrobenthic invertebrate totally were ranged from 1.33 between the Shatt Al - Arab river and east Hammar marsh to 1.54 between the Shatt Al - Arab river and marsh of Al - Chebaeish.Jaccard Similarity index for each main macrobenthic invertebrate group for each stations was calculated.The values of index for annelids were ranged from( 0 - 89)% the lowest value recorded between Al - Burgah and Al - Sibitia stations and the highest value between Al - Salihia and Multaqaa of Al - Mashab and Al - Sallal stations, for the aquatic insects the values of index between (8.3 - 46.7)% the lowest value between Al - Salihia and Al - Sibitia and the highest value between Al - Mohammediat and Al - Badria stations, for the crustaeceans values ranged(14.3 - 77.8)% the lowest value between Al - Mohammediat and Al - Badria stations while the highest value between Al - Sibitia and Al - Badria,and for mollusks were between(18 - 80)% the lowest value between Al - Mohammediat and Al - Burgah stations while the highest value between Multaqaa of Al - Mashab and Al - Sallal and Abo Sobat stations.The index for all macrobenthic invertebrate groups totally also calculated and its values were from (15 - 58.5)% recorded between Al - Mohammediat with Al - Sibitia stations and between Al - Mohammediat with Al - Salihia stations respectively.Bray - Curtis index was calculated monthly for each main macrobenthic invertebrate group in each stations .Also the Bray - Curtis index was calculated annually between stations and the results refer that the highest values as the following : 0.993 for the annelids between Al - Badria and Al - Sihaqee stations, 1 for the crustaceans between Al - Salihia and Al - Sibitia stations, and 0.96 for the mollusks between Al - Sibitia and Abo Sobat stations.The highest value for all benthic group totally was 0.983 between Al - Salihia and Abo Sobat stations.On other hand Bray - Curtis index was calculated for all macrobenthic invertebrate groups totally between the three aquatic habitats and the values were ranged from 0.5 recorded between the wetlands of um Al - Shoowaich and Al - Chebaeish to 0.875 recorded between the east Hammar marsh and wetlands of um Al - Shoowaich.The Canonical Correspondence Analysis (CCA) by using Canoco application revealed the clear effect of different ecological factors on numbers of the individuals and species of each group of macrobenthic invertebrate in all stations during the study period.

تاثير المركبات الثانوية لاوراق نبات كف مريم Vitex agnus - castus L.مع مبيد بولو500 في بعض جوانب الاداء الحيوي لذبابة التبغ البيضاءBemisia tabaci (Genn.) (Hemiptera : Homoptera : Aleyrodidae) == Effect Of secondary Plant compounds of Vitex agnus - castus L. With PoLo500 Insecticide in Some Biological aspects of Tabacoo Whitefly Bemisia tabaci (Genn.) (Hemiptera : Homoptera : Aleyrodidae

Author name: رشا حطاب عبد الله الاسدي
Supervisor name: ناصر عبد علي المنصور
General topic: Biology
Specific topic: Zoology - Immunology
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية لتقييم كفاءة المستخلصات النباتية والمركبات الفعالة لاوراق نبات كف مريمL. Vitex agnus - castus في الاداء الحيوي لذبابة التبغ البيضاء Bemisia tabaci (Genn.) من خلال حساب معدلات الهلاك عند كل معاملة وبتراكيز وفترات زمنية مختلفة من دور البيضة الى دور الحشرة الكاملة وحساب معدلات الهلاك التراكمية للادوار غير البالغة وحساب معدلات الهلاك باستخدام مبيد بولو 500 واوضحت النتائج ان النبات والمبيد المستخدمين في هذه الدراسة لهما تاثير كبير في حياتية ذبابة التبغ البيضاء وكما يلي : A - المستخلصات النباتبة : تمثل نسب هلاك البيوض اقل المستويات مقارنة مع الاطوار الاخرى اذ كان مستخلص خلات الاثيل الاكثر تاثيرا ، اذ بلغ معدل الهلاك %83.67 يليه المستخلص الكحولي 70.60% واقل المستخلصات تاثيرا المركبات الفينولية بلغ معدل هلاكها 48.5% .كانت اغلب المستخلصات المستخدمة ذات تاثير كبيرفي هلاك الطور الحوري الاول ، اذ بلغ اعلى معدل للهلاك باستخدام المستخلص الكحولي وبلغ 95.87% يليه خلات الاثيل بمعدل 78.52% واقل معدل للهلاك كان عند استخدام المستخلص المائي بمعدل 49.57 %.تفوقت المستخلصات النباتية الاتية في الطور الحوري الثاني وهي : الكحولي والهكسان وخلات الاثيل والقلويدات وبمعدلات هلاك 96.33 و89.74% و76.93 و72.27 %على التوالي،اما المستخلص المائي فكان الاقل تاثيرا اذ بلغ معدل الهلاك 55.9%.اما الطور الحوري الثالث كانت اغلب المستخلصات النباتية والمركبات الفعالة ذات تاثير كبيرفي معدل هلاك هذا الطوراذ بلغت معدلات الهلاك93.29% للمستخلص الكحولي و82.83% لمستخلص خلات الاثيل و75.55% للقلويدات واقل معدل للهلاك سجل عند استخدام المستخلص المائي وبلغ %59.54 .سجل الطور الحوري الرابع (العذراء) اعلى معدل للهلاك عند استخدام المستخلص الكحولي وكانت النتيجة 91.41% تليه المركبات الفينولية 89.28% واقل معدل للهلاك سجل عند استخدام المستخلص المائي بلغ %59.12 .وكان اعلى معدل لهلاك الحشرة الكاملة عند استخدام المركبات القلوانية بمعدل90.94% تليها المركبات الفينولية بمعدل 89.93% وخلات الاثيل بمعدل89.85 % والكحولي 87.69% والهكسان 80.17% واقل معدل للهلاك عند استخدام المستخلص المائي وبلغ معدل الهلاك %69.03. - B معدل الهلاك التراكمي : اوضحت النتائج تفوقا واضحا للمركبات القلوانية في معدل الهلاك التراكمي للادوار غير البالغة لذبابة التبغ البيضاءB. tabaci يليها مستخلص الهكسان ومستخلص خلات الاثيل والمستخلص الكحولي واقلها كان المستخلص المائي . - C مبيد بولو 500 : تبين من خلال النتائج ان استخدام مبيد بولو500 له تاثير كبير في معدل هلاك ذبابة التبغ البيضاء من دور البيضة لحين الوصول الى الحشرة الكاملة ،اذ بلغت معدلات الهلاك كما يلي : البيوض 58.51% والطور الحوري الاول 44.70% والطور الحوري الثاني 64.41% والطور الحوري الثالث 70.49% والطور الحوري الرابع (العذراء) 73.48% واخيرا الحشرة الكاملة 72.30 %. | The current study was conducted in order to evaluation efficiency of plant extract and effective compounds to plant Vitex asgnus - castus L. on the biological performance of Bemisia tabaci(Genn.)whitefly through calculating mortality rate for each treatment with different concentration and period from egg stage until adult stage and calculate the cumulative mortality rate for immature stages and calculate mortality rate by use Polo500 insecticide Ressults demonstrated that the plant and insecticide used in current study had more effective important role in life of the B. tabaci as follow A - Plant Extract Mortality rate of egg : represent ratios of egg mortality in the lower levels compare with other stages , mortaiity rate of ethyle acetate extract has more effective (83.67%) than alcohol extract mortality rate (70.60%) and lower extract effective was phenol compounds mortality (48.50%) First Nymphal instar : in this instar most of used extracts had more effective in mortality of this instar, more mortality rate were by alcohole extract (95.87%) the ethyl acetate (78.52%) and lower mortality rate was by aqueous extract (49.57%) Second Nymphal insrar : most effective was to following plant extracts; Alcohol, Hexan , Ethyl acetat , Alkaloid with mortality rate (96.33%) , 89.74% , 76.93% , 72.27% Aqueous extract has the lower effective mortality rate 55.90% Third Nymphal instar : most plante extracts and effective compounds had large effect in mortality rate in this stage (93.29%) for alcohol extract , 82.83% for ethyl acetat ,75.55% for alkaloid and lower effective was to aqueous extract 59.54% Fourth Nymphal instar ( pupa) : in this stage alcohol extract was more effective in mortality rate 91.41% , then phenol compound 89.825 and lower perdition average was to aqueous extract 59. 12% .Adult : the highest mortality rate was when used alkaloid compounds 89.93% , then ethyl acetat 89.85% , alcohol 87. 69% , Hexan 80.17% and lower average to aqueous extract 69.03% .B - The accumalative perdition : Results demonstrated more effective to alkaloid compounds in inmature stage of B.tabaci , then to hexan extract , ethyl acetat , alcohol extract and lower average was to aqueous extract .C - POLO500 Insecticide : Results show this insecticide has significant effect in motality rate of B. tabaci starting from egg to adult . egg 58.51% , first instar 44.70% ,second instar 64.41% , third instar 70.49% , pupa 73.48% and adult 72.30%

دراسة التغيرات النسجية المرضية في اعضاء سمكتي البلطي الزيلي Coptodon zillii والكارب البروسي Carassius auratus في بعض انهار ابي الخصيب وعلاقتها ببعض العناصر الثقيلة == Study of Histopathological changes in organs of fish, Coptodon zillii and Carassius auratus in Abo Alkhaseeb Rivers and relationship with some type of heavy metals

Author name: خالد عبد الصمد عبد الرضا السلمي
Supervisor name: علي عبد اللطيف عبد الحسن العلي
General topic: Biology
Specific topic: Zoology - Histology
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: تضمنت الدراسة الحالية جمع نوعين من الاسماك النهرية هي سمكة البلطي الزيليCoptodon zillii والكارب البروسي Carassius auratus من اربعة انهار في قضاء ابي الخصيب وهي نهر( السراجي وحمدان ومحيلة وابو مغيرة )وعلى فترتين ,امتدت الاولى من شهر حزيران 2013 ولغاية شهر تشرين الثاني 2013 وامتدت الثانية من شهر كانون الاول 2013 ولغاية شهر مايس 2014 . اجريت الدراسة الحالية بمحورين شمل المحور الاول دراسة التغيرات النسجية المرضية في اعضاء الغلاصم والكبد والكلية والعضلات وللسمكتين اما المحور الثاني فتضمن دراسة تراكم اربعة من العناصر الثقيلة(الرصاص والنحاس والنيكل والكوبلت) في انسجة الكبد والعضلات وذلك لغرض ايجاد علاقة بين المحورين . قد بينت الدراسة النسجية للاعضاء المدروسة ظهور تغيرات نسجية مرضية اختلفت في شدتها بين نوعي الاسماك اذ تبدو انسجة اسماك الكارب اكثر تضررا من انسجة اسماك البلطي فضلا عن اختلافها بين افراد النوع الواحد , ففي الغلاصم تمثلت التغيرات المرضية بتغير شكل الصفائح الغلصمية الثانوية وفرط تنسج hyperplasia النسيج االظهاري وانفصاله detachment ولوحظ تضخم hypertrophy الخلايا الظهارية وانتفاخها swelling وتضخم وزيادة اعداد خلايا الكلورايد فيها فضلا عن ظهور فرط تنسج الغضروف في الهيكل الساند للخيط الغلصمي الذي رافقه في بعض الاحيان تجمع خلايا بيضوية طرفية النواة في غلاصم اسماك الكارب . في الكبد تمثلت التغيرات المرضية باحتقان congestion اشباه االجيوب الكبدية وتليفات fibrosis في نسيج الكبد وتنكس degeneration وتنخرnecrosis الخلايا الكبدية وتغيرات نووية تمثلت بانحلال النواة وتكتل مادتها الكروماتينية karyopiknosis ولوحظ وجود ودمات oedema وتراكم مواد غير حية بنية ووردية اللون داخل وخارج الخلايا الكبدية . اما في الكلية فقد شملت التغيرات المرضية النسجية جميع مكوناتها اذ تمثلت بتنكس الخلايا الظهارية للنبيبات الكلوية وانتفاخ بعض منها مما يؤدي الى ضيق تجويف النبيب ولوحظ حدوث انسلاخ بطانة بعض النبيبات الكلوية , وظهر فرط تنسج النبيبات الكلوية في كلية اسماك البلطي فقط بشكل كتل كبيرة بيضوية الشكل , ولوحظ وجود مواد غير حية في النسيج المكون للدم يحاط البعض منها بمحفظة ليفية من عدة طبقات في سمكة الكارب ,اما في الكبيبات فقد لوحظ احتقان وتوسع aneurism اوعيتها الدموية, وشوهد ضمورatrophy بعض الكبيبات وتضخم بعضها الاخر مما يؤدي الى توسع او تضيق حيز محفظة بومان. تمثلت التغيرات المرضية النسجية في الامعاء بتنكس خلايا النسيج الظهاري وتنخر قمم الزغابات ولوحظ ان التنكس والتنخر يصل في بعض العينات الى النسيج الضام للصفيحة الاصيلة وشوهد ارتشاح infiltration الخلايا البدينة mast cells باعداد كبيرة في الطبقة تحت المخاطية لامعاء اسماك البلطي وارتشاح انواع اخرى من الخلايا الالتهابية في اسماك كلا النوعين , وكثيرا ما شوهد تاثر الخلايا العضلية في طبقة العضلات لجدار الامعاء. اما انسجة العضلات فقد شهدت ظهور العديد من التغيرات تمثلت بتنكس اسفنجي spongy degeneration يتبعه تنخر الخلايا العضلية وكثيرا ما شوهد تعرج الليفات العضلية وانعدام التخطيط العرضي . قد تعزى هذه التغيرات الى تلوث مياه الانهار المدروسة بمختلف الملوثات ومنها المعادن الثقيلة وذلك من خلال قياس التراكم الحيوي لهذه العناصر في عضلات واكباد هذه الاسماك واكد وجود هذه العناصر تقنية الكيمياء النسجية اذ لوحظ ان المعادن المتراكمة في النسيج تعطي انعكاسات لونية مختلفة بحسب نوع العنصر الثقيل ,و لوحظ عند قياس التراكم الحيوي ان معدلات تراكم بعض هذه العناصر(الرصاص والكوبلت) في انسجة العضلات يفوق الحدود المسموح بها عالميا . اختلف تراكم هذه العناصر من عضو الى اخر ومن نوع الى اخر ومن فترة الى اخرى ,فقد وجد ان معدلات تراكم عناصر الرصاص والنيكل والكوبلت في العضلات كان اعلى من معدله في الكبد وبفرق معنوي عند مستوى احتمالية p<0.05 في حين اظهر النحاس نتيجة معاكسة اذ كان معدل تراكمه في الكبد اعلى من العضلات وبفرق معنوي عند مستوى احتمالية p<0.05 , اما بالنسبة لفترتي الدراسة فقد سجلت الفترة الاولى في كبد سمكة البلطي معدلات تركيز اعلى من الفترة الثانية لكل من عناصر النحاس والنيكل والكوبلت وبفرق معنوي عند مستوى احتمالية p<0.05 . اما في مياه الانهار المدروسة فقد كان اعلى تركيز بلغه عنصر الرصاص 602 مايكروغرام\لتر واقل تركيز له 141 مايكروغرام\لتر,واعلى تركيزلعنصر النحاس كان 77 مايكروغرام\لتر واقل تركيز 25 مايكروغرام\لتر, واعلى تركيز لعنصر النيكل كان 392مايكروغرام\لتر واقل تركيز له214 مايكروغرام\لتر,واعلى تركيز لعنصر الكوبلت كان 173مايكروغرام\لتر واقل تركيز72مايكروغرام\لتر | The current study includes collection of two species of fish. a tilapia fish (Coptodon zillii) and Prussian carp ( Carassius auratus) from four rivers in Abu Alkhaseeb region which are Sarraji, Hamdan, Mhella and Abu Mugherra River at two periods, the first period starts from June 2013 till November 2013 and the second period from December 2013 to the end of May 2014. After bringing the fish to the lab dissecting to rescet excised organs of the gills, livers, kidneys , intestines and muscles. the current study have been performed on two axis .The first axis includes the study of histopathological changes in the organs above for two species The second axis guarantees the study of the bioaccumulation of four heavy metals (lead ,copper, nickel ,cobalt) in the tissues of the liver and muscles for the purpose of finding a relationship between the two axes. The histological observations showed histopathological changes different in severity between the two specis of fish , the tissue of C.auratus seems to more be affected than the tissue of C.zillii , In the gills, the pathological changes are represented in the shape changes of secondary lamella of gills , hyperplasia and detachment of epithelial tissue . Hypertrophy and swelling in epithelial cells , hypertrophy and increase the number of chloride cells where noted . Hyperplasia cartilage in Bracket structure of gills thread which coincide with some gathering terminal oval cell nucleus often in gill, carp was seen. In the liver has represented pathological changes like congestion of liver sinusoid , fibrosis in liver tissue , degeneration and necrosis of hepatocytes and the nuclear changes represented in the dissolution of the nucleus and conglomerate chromatin material ( karyopiknosis), the appearance of some oedema and accumulation of non - living material inside and outside the hypatocytes . In kidney ,the histopathological changes included represente by epithelial cells degeneration of the renal tubules and swelling some of them leading to a narrow tubules cavity, also observed shedding the epithelial layer lining of some renal tubule, while in the glomeruli, congestion and aneurism its veins atrophy of some glomeruli was obsererved and that leads to expansion or narrowing Bomans space . In addition non - living materials in the component tissue of blood were observed, some of which are surrounded by a fibrous capsule of several layers in C.auratus kidney only. Some of non - liver materials in the cytoplasm of some of renal tubule cells were seen, while, intestinal histopathological changes were represented by degeneration of epithelial cells and necrosis of villi peaks and it was observed that the degeneration and necrosis reach in some samples to the connective tissue and often spotted muscle cells affected in the muscle layer of the intestinal wall. Also it is observedin a large numbers of mast cells infiltration in the submucosa layer of the gut C.zillii and infiltration other types of inflammatory cells in both fish species, while muscle tissue were less affected than the other organs. There were several changes represented by spongy degeneration followed by necrosis of muscle cells , and often were seen in the limping of muscle fibers and the lack of cross - planning in this muscle. These histological changes were due to water pollution of rivers under studing by various pollutants such as heavy metals that were included in thestudy and that by measuring the bioaccumulation of heavy metals in the tissues of the muscles and liver of these fish and was assured that by Histochemistry technique as it was observed that the accumulated metals in the tissues give different reflections of color by heavy element type. It was observed that when measuring bioaccumulation , the accumulation rates of some of these elements (lead and cobalt) exceeds the permitted globally border. It was observed that the accumulation of these elements vary from one organ to another and from one specis to another and from one period to another, it was found that the rates of accumulation of elements ;lead , nickel and cobalt in the muscle were higher than that in the liver and at significant difference of 0.05 level of probability, while copper showed the opposite conclusion, as its rate of accumulation in the liver was higher thane at significant differenc of 0.05 level of probability. Regarding the two periods of the study, the first period recorded higher concentration in the liver of C.zillii than the second period for copper ,Nickel and cobalt elements at significant 0.05 difference are level of probability. . The rates of concentration of heavy elements that included in the study of the water of the rivers were nearly the same to former studies, as the highest concentration recorded for lead was 602 micrograms \ liter and the less concentration was 141 micrograms \ L, while the highest concentration of copper was 77 micrograms \ liter and less concentration was 25 micrograms \ L. The highest concentration of the nickel was 392 micrograms \ liter and its less concentration was 214 micrograms him \ l. The finally highest concentration of cobalt was 173 micrograms \ liter and its less concentration was 72 micrograms \ L

الكساء الخضري والتنوع الاحيائي النباتي في منطقة الصحراء الجنوبية في محافظة البصرة / جنوب العراق == Vegetation and Plant Biodiversity in the Southern Desert in Basra Governorate, Southern Iraq

Author name: حيدر راضي مالح حسين
Supervisor name: عبد الرضا اكبر علوان المياح
General topic: Biology
Specific topic: Plant - Environment
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: تناولت الدراسة الحالية الغطاء النباتي والتنوع الاحيائي والمجتمعات النباتية في منطقة الصحراء الجنوبية ضمن محافظة البصرة, وتم دراسة الخصائص الكمية والنوعية للغطاء النباتي , فضلا عن خصائص الترب والهواء في 12 محطة, 6 منها خصصت للمسح الشهري للانواع للفترة بين كانون الاول 2012 وكانون الاول 2014.تم دراسة الخصائص الفيزيائية والكيميائية للعوامل البيئية في منطقة الدراسة وشملت حساب قيم درجة حرارة الهواء والرطوبة النسبية, وكذلك دراسة خصائص الترب ومنها قوام التربة ودرجة حرارة التربة ورطوبة الترب والاس الهيدروجيني والمادة العضوية والتوصيلية الكهربائية وقيم الملوحة, وتبين انها اظهرت تباينا موسميا في المحطات الستة. اوضحت الدراسة الحالية وجود 185 نوع و136 جنس تعود الى 41 عائلة, منها 159 نوع و115 جنس و36 عائلة تعود الى مجموعة نباتات ذوات الفلقتين, في حين ضمت مجموعة نباتات ذوات الفلقة الواحدة 26 نوع تقع في 21 جنس و5 عائلات, وكانت اغلبها نباتات حولية حوالي 66% , بينما كانت نسبة النباتات المعمرة 44 %, كما تم تسجيل حوالي 20 نوع لاول مره في مناطق الدراسة, كما اكدت التوزيع الجغرافي وسجلت انتشار العديد من الانواع في مناطق لم تسجل فيها سابقا.وبينت دراسة اشكال الحياة في منطقة الدراسة ان مجموعة Therophytes قد امتلكت اعلى نسبة بلغت 71.19%, بينما ضمت مجموعة ال Geophytes اقل نسبة بلغت 4.3 %. كما تغايرت الاصول الجغرافية النباتية Chorotype للانواع,عكست اهمية الموقع الجغرافي لمنطقة الدراسة, وسجلت العناصر ثنائية وعديدة المناطقBi & Pluri regional 70.7 elements % منها, وشكلت العناصر الصحراوية - العربية Sahara - Arabia elements نسبة 20.54%.وبينت النتائج ان اكبر العائلات انتشارا وتوزيعا وبعدد الانواع في منطقة الدراسة هي العائلة المركبة Compositae التي تعد اكبر العائلات في المنطقة, ثم العائلة الصليبية Cruciferae , والعائلة النجيلية Gramineae , والعائلة البقوليةFabaaceae , والعائلة الرمرامية Chenopodiaceae , كما تبين ان الاجناس Astragalus وPlantago وErodium كانت اكبر الاجناس بعدد الانواع في منطقة الدراسة, واظهر ت دراسة التوزيع الجغرافي ان بعض الانواع سجلت انتشارا واسعا في مناطق الدراسة ومنها Hammada salicornica Moq. وRhanterium epapposum Oliv.و Stipa capensis Thunb. وIflago spicata (Forssk) Sch. Bip. وFilago pyramidata L. وSpegularia diandra (Guss.) Heldr & Sart. وStrigosella grandiflora ( Bunge.) Boch. .كما تضمنت الدراسة وصف وتمييز المجتمعات الرئيسة وتحديد مناطق انتشارها في مناطق الدراسة وضمت Hammadetum salicornici وCornulacetum auchraeو Rhanterietum epapposi وZygophyllietum propinqun وAstragalietum spinosi وSalsoletum setiferaeو Convolvulietum oxyphylliو Salsoletum rosmarini وStipetum capensiو Ziziphetum nummulariaeو Teucriutum oliveriو Stipagrosis plumosi وLycetum barburae وSuaedetum aegyptiaci . واظهرت الدراسة التغيرات الموسمية في طبيعة الغطاء النباتي وتاثر السيادة بالتغيرات الموسمية في الظروف المناخية والتي ساهمت في ظهور المنطقة بعدد من المظاهر الموسمية للغطاء الخضري ( Seasonal Aspect) . وبينت نتائج الدراسة الحالية وجود تباين موسمي ومكاني بين غالبية محطات الدراسة في قيم ادلة الكثافة والوفرة والتغطية والتنوع والسيادة والتجانس والغنى النوعي والتشابه والكتلة الحية والتصاحب والاهمية.

دراسة تعدد الاشكال الوراثية لجين GSTPI في مرض السكري من النوع الثاني لعينة مرضى في محافظة البصرة وعلاقتها بدهون مصل الدم == Genetic Polymorphism of Gultathion - S - Transferase GSTPI in Type2 Diabetes Mellitus and it's Correlation with Blood Serum lipid Profile in Basrah Province

Author name: حنين صباح عبد الصمد
Supervisor name: فائزة عبد الوهاب احمد
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was carried out to investigate the relationships between the T2DM patients and the genotypes of glutathione S - transferase P1, and the measurements of blood serum lipid profile of patients and control. The study included 60 blood samples from patients and 40 blood sample from controls of Iraqi individuals. The sixty individual with T2DM were diagnosed according to the American diabetes Association criteria (American diabetes Association, 2007), their age ranged were between 35 - 75 years randomly selected from those attending the Diabetes Center /Al - Moanaa Hospital for treatment with history of hyperlipidemia and hypertension. Sera and blood were used as sample, The measured parameters in serum included (Biochemical testes) : Fasting Blood Glucose (FBG), Total Cholesrtol (TC), Triglyceride (TG), low density lipoprotein (LDL), High density lipoprotein (HDL), Very high density lipoprotein (VLDL). In Addition to Body Mass Index (BMI) .Moreover, the correlation between genotyping of glutathione S - transferase P1 and all studied parameters was carried out. Molecular studies involved DNA extraction and Polymerase chain reaction (PCR) - Retraction fragment length polymorphism (RFLP). to investigate the genetic polymorphism of glutathione S - transferase pi gene class for individuals of this study .Molecular study was conducted in the laboratory of molecular Biology, Department of Biology for pure Sciences college at Basra University .The result showed that Sex, BMI, HDL, LDL and VLDL values for patients and control were not significant while for the level of FBG in patients and control it showed significantly difference (P=0.000). This study revealed significant Positive Correlation between cholesterol and triglyceride (P=0.01). while the Correlation coefficient between Triglyceride & HDL,VLDL individually also between HDL and LDL ,VLDL and LDL with VLDL individually was not significant furthermore FBG and Cholesterol showed positive significant Correlation .The result of genetic polymorphism of GSTPI distribution among studied groups showed rise developing risk of Type 2 Diabetes in patients about 2 - fold in carries heterozygous Ile/Val genotype with an ( OR=2.90; 95% Cl= 1.077 - 7.827, p=0.31) and allele frequency 0.69.,and there is an increased risk of Type 2 Diabetes in patients about 2and half - fold in carries of The homozygous Val/Val genotype in patients with an (OR=2.58, 95%CI=0.625 - 10.662, p=0.171). the heterozygote distribution (2Pq ) of Patients was 0.427 while in control 0.268 and it was not stable according to Hardy - Winderg question.According to data mentioned above the GSTPI genotype polymorphism may play an important role in the pathogenesis of T2DM while its effect on all lipid profile indicator did not show any significant difference.

دراسة جزيئية لتعدد الاشكال الوراثية لجين TAS2R38 (جين تذوق مادة PTC) بين سكان محافظة البصرة وعلاقتها بمرض السكري == A Molecular Study of TAS2R38 (PTC Tasting Gene) Polymorphism Among Basrah Population and It's Association With Diabetes Mellitus

Author name: حسنة عامر مهوس
Supervisor name: فائزة عبد الوهاب احمد | اسعد يحيى عايد
General topic: Biology
Specific topic: Microbiology - Genetics
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: This study dealt with the genetic polymorphism of Phenylthiocarbamide (PTC) tasting from phenotypic and genotypic (Molecular of TAS2R38(P49A) gene ) prospective,among sample of Basrah Population from 2014 - 2012. Also, a comparative Molecular study for the gene TAS2R38(P49A) and some biochemical parameters was made in a group of Diabetic patients for the first time in Iraq /Basrah and the world .Phenotypic study using the gradient dilutions of PTC substance method, was established among 317 individual : 96Males and 221 Females from different regions of Basrah. Increased sensitivity with low threshold 6 of Males than Females 5 was observed which indicate that the Males were more sensitive than Females in this region from Iraq .However, the tasters percent among Females still higher73.3% than Males72.9% ,and the whole population 73.2% as well .This study recorded different tasters percent in different regions : 62.1%,71.8% ,60.6%, 82.3% for the North ,AL - Zubair and Safwan, Abu - ALChaseab and AL - Fao and the center of the city respectively, although ,the whole population of Basrah found to be under Hardy - Weinberg Equilibrium . Allelic frequencies according to phenotypic analysis showed : recessive allele t had higher frequency (0.53) than dominant allele T (0.47), with elevated Heterozygous frequency Tt among all studied regions and the whole population(0.53) .The results recorded also , high BMI for non tasters tt as a whole and for both sexes in this phenotype : 28.32 ,28.26 for males and females respectively. While it had the lowest value among heterozygous 23.40 ,Statistical analysis for phenotypic study of this locus reveald an additive effect of the two alleles T,t by 81.33% with responsibility of 83.10% for the phenotypic variation found in this trait . Genetic (Molecular) analysis was done for comparative individuals and diabetic patients by sequencing the hTAS2R38(P49A) the PTC gene, among 127 individual : 59 healthy individual as a comparative group and 68 diabetic patients from both sexes and same age ranges. The results recorded a C/G allele in the position of the first SNP (145 C/G) which encode for Proline amino acid (C) or Alanine amino acid (G) : CC , CG refer to Tasters genotypes while GG refer to Non - tasters genotype. High frequency of C allele as compare to G among both healthy people and diabetic patients : 0.55, 0.53 for both respectively . Frequency of heterozygous genotype CG recorded a significant access as 0.76 among the comparative group which may implies a selective heterozygosity advantage among Iraqi population at this region. A significant differences in genotypic distributions was recorded between comparative and diabetic groups with high incidence of non tasters phenotypically tt 41% and genotypically GG 20.5 % among diabetic patients compare to healthy people : 25 % , 7% respectively . Interestingly non taster GG genotype had the lowest BMI among diabetic patients compare to othertwo taster genotypes CC, CG respectively. . Study of Genotype - Phenotype Association revealed significant correlation (p<0.0001) between them among comparative group as 86.9% and diabetic group as 48.8%. A comparative study of Biochemical analysis betweendiabetic and non diabetic individuals from different genotypes for TAS2R38 (P49A) gene showed significant elevated value of cholesterol among diabetic from the GG genotype 191.85 ± 6.57 mg/dl with non significant elevated values of Hb1c 8.93 ±1.77 and triglyceride 149.57±6.53 mg/dl. However, all these parameters were significantly more higher among diabetic than non diabetic . Significant differences(P<0.05) were recorded between diabetic and non diabetic according to their thyroid hormones levels with low values of T3 , T4 and high values of TSH among diabetic than non - diabetic. TSH hormone recorded its highest significant values among diabetic of CC genotype with 4.39 ± 0.16 μUI/ml as compare to the other two genotypes heterozygous CG and recessive homozygous GG . Although non significant , T4 recorded its lowest values among diabetic of the CC genotype .However, significantly (P <0.05 ),T3 hormone found to have lower values in the two homozygous genotypes; CC genotype for diabetic 0.54± 0.03 ng/ml and non diabetic 0.53±1.64 ng/ml ; GG genotype : 0.72± 0.04 ng/ml ; 1.82± 0.59 ng/ml for diabetic and non diabetic respectively . while individuals with CG genotype found to be non significantly different .Lastly it could be concluded that this trait may have some effect on BMI and may play a role the variation found among peoples in biochemical parameters (cholesterol, thyroid hormones ) changing's especially among diabetic patients. Ministry of High Education and Scientific Research Basrah University/ College of Education for Pure Science Department of Biology A Molecular Study of TAS2R38 (PTC Tasting Gene) Polymorphism Among Basrah Population and It's Association With Diabetes MellitusA Thesis submitted to the Council of the College of Education for Pure Science/ University of Basrah as a partial fulfillment of the requirements for the degree of Philosophy of Doctor of Science in Biology / Genetics By Hasna Amir Mohaus BSc. Biology / 1994MSc. Biology / 1998 Supervision by Assist.Prof.Dr. Prof.Dr.Faizah A.

دراسة بعض التغيرات الفسيولوجية والنسجية لسم الافعى ذات الحراشف المنشارية (افعى سيد دخيل Echis carinatus) في الجرذان المختبرية == A study of some physiological and histological effects of venom Saw Scaled Viper (Said Dakhil) Echis carinatus in laboratory rats

Author name: بيداء ريحان علي الموسوي
General topic: Biology
Specific topic: Zoology - Histology
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was designed search of some physiological and histological effects of the venom of Echis carinatus sochureki (Said Dakhil snake) in male and female rats, and the role of antivenom (anti - venom) in the treatment of these effects. The while study was divided into two parts, the first part included the effect of [0.04 mg/kg (the second group) and 0.08 mg/kg (the third group)] of venom in rats. While, the second part related with the effect of [0.25ml/animal (the second group) and 0.5ml/animal (the third group)] antivenom in male and female rats treated with 0.04mg/kg and 0.08mg/kg of venom. After 24 hours of injection, blood samples collected for the hematological and biochemical parameters, and taking the right epididymis and epididymis left removed to examine the count and maliformations of sperm. Also, liver, kidney, testes and ovary were collected for sections. The results of the present study showed a significant decrease (P≤0.05) in RBC in the third group (0.08) mg / kg when compared with the first group (control) and second group (0.04) mg / kg. Also was there a significant decrease in HCT and MCV in the second and third groups compared with the first group, while there was a significant increase in the platelets of the third group compared with the first and second groups in in both sexes. The results indicated a significant decrease in RBC in the second and third groups compared with the first group, also a significant decrease in HCT in the second and third groups compared with the first group. The results showed a significant increase in the HCT in the second group compared with the third group. The results showed a significant decrease in the MCV in the third gr oup compared with first and second groups in female rats. The present study (P≤0.05) showed a significantdecrease in total WBC, lymphocytes and neutrophils in the second groups (0.04) mg / kg and the third (0.08) mg / kg, and a significant decrease in monocytes, acidiophils and basophils of the second and third groups compared with the first in the male rats. The results indicated a significant decrease in neutrophil in the second and third groups compared with the first group, and a significant decrease in lymphocytes, monocytes and acidiophils of the third group compared with the first and second groups in the female rats. The present study showed a significant increase (P≤0.05) in the level of glucose and urea in the third group (0.08) mg / kg compared with the first (control) and second (0.04) mg / kg groups. There was a significant increase in creatinine and albumin in the second and third groups compared with the first group, also a significant decrease in the total protein, cholesterol and HDL in the second and third groups compared with the first group.There was a significant decrease in the level of TG, LDL and VLDL in the third group compared with the first and second groups, The results showed a significant decrease in ALT and AST in the third group compared with the first and second groups, and a significant increase in the ALP in the third group compared with the first and second groups in the male. The hormonal study showed a significant decrease (P≤0.05) in the level of FSH, LH, testosterone and estrogen in the second and third groups compared with the first group in male and female rats. The present study a significant decrease (P≤0.05) in the count of sperms in the second and third groups compared with the first group, while, there was a significant increase in the maliformations of sperms in the third group compared with the first and second groups. The present study showed a significant decrease (P≤0.05) in the level of immune globulins (IgA - IgG - IgM) in the second and third groups compared with the first group in both sexes. The results of the effect of (0.5 - 0.25) ml / animal antivenom in male and female rats treated with (0.04) of snake venom showed a significant decrease in RBC and HCT in the second and third groups compared with the first groups,and there was a significant increase in the MCV in the second and third groups compared with the first group in male. The study results showed a significant increase in RBC, HCT and MCV in the second and third groups compared with the first group in the female rats.The current study showed a significant decrease (P≤0.05) in total WBC of the second group compared with the first and third groups, and a significant decrease in the lymphocytes and monocytes in the second and third groups compared with the first group, also the results showed a significant increase in total WBC in the second and third groups compared with the first in the male. Also, found a significant decrease in lymphocytes and monocytes in the secon d and third groups compared with the first, it was observed a significant rise in monocyte in the first group compared with the second and third groups. The results showed a significant increase in the percentage of granular white blood cells in the second and third groups compared with the first group in the female rats.Results of the present study indicated a significant decrease (P≤0.05) in the level of glucose, urea, albumin and total protein in the third group compared with the first and second groups, and there was a significant increase in the level of cholesterol HDL - VLDL in the third group compared with the first and second groups, also a significant decrease in TG in the second group compared with the first and third groups, The results showed a significant decrease in the level of HDL in the second and third groups compared and second groups with the firstgroup. the results showed a significant increase in the level of albumin and total protein in the third group compared with the first and second groups, and the results indicated a significant in increase cholesterol level, LDL and VLDL in the third group compare with the first and second groups, There was a significant decrease in the level of TG in second group compared with the first and third groups, and a significant decrease in the level of HDL in the second and the third groups compared with the first group in the female rats.The results indicated the effect of (0.5 - 0.25) ml / animal antivenom in male and female rats treated (0.08) mg / kg of snake venom, showed a significant decreased (P≤0.05) in RBC, HCT , MCV, lymphocyte and monocyte in the second and third groups compared with the first group, a significant decrease MCH in the second group compared with the first and third groups in male rats, showed a significant decrease in RBC HCT and MCV in the second and thirdgroups compared with the first group.The present study showed a significant decrease (P≤0.05) in the level ofglucose and urea, ALT and ALP in the third group compared with the first and second groups, also a significant decrease in the level of creatinine, LDL, VLDLand AST in the second and third groups compared with the first group, and asignificant increase in the level of albumin, total protein and HDL in the thirdgroup compared with the first and second groups. a significant increase in levelof cholesterol and TG in the second and third groups compared with the firstgroup in the male rats, While showed a significant decrease in level of glucose,urea and ALT in the third group compared with the first and second groups,there was a significant decrease in the level of creatinine, LDL, VLDL, AST and ALP in the second and third groups compared with the first group, a significant increase in albumin and total protein in the group third compared with the first and second groups, while a significant increase in level of cholesterol, TG and HDL in the second and third groups compared with the first group in female.The histological results showed changes in the liver of male and female rats treatment I n venom snake and constrictions consisted obtaining inflammation and blood congestion and enlarged nuclei of hepatic cells and degeneration in the cytoplasm and necrosis of liver cells, as well as, changes in the kidney tissue such as having a bleeding and hyperplasia and congestion blood and inflammation and analyze the entire glomerulus and necrosis of the cortical cells.The current study showed the incidence and clear changes in testicular tissue treatment venom snake of obtaining vascular congestion and analyze the interconnection fabric between the tubule and the decay of some nuclei and abnormalities tubule and testicular and shrinking and analyzes and the presence of multiple nuclei giant cells in the wall of tubule.The current study for histological changes in the ovary as sections showed the presence of large numbers of corpus luteum and the decrease numbers of ovarian follicles and the disappearance of the evolutionary stages of ovarian cysts.

تاثير PTU على بعض الجوانب النسيجية والفسلجية في الاجنة والاناث الحوامل Rattus norvegicus == Effect of PTU on some aspects of histological and physiological in fetus and pregnant rats(rattus norvegicus

Author name: انوار ناذر صيوان
Supervisor name: مها خليل الملاك | علاء عبد الخالق حسين
General topic: Biology
Specific topic: Zoology - Histology
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: Aim of study was to evaluated the effects of propylthiouracil (Ptu) drug on both maternal and fetus rats, used experimental animals (Rattus norvegicus) and after housing, breeding and adapting, (50) of virgin females choosen, isolated and then divided into two groups each group contain (25)rats, The mean weight of the animals was(200 ±50) gm and (8 - 10) week of age. Females on first group regarded as control and treated with distilled water while animals in second group were treated with (Ptu)dose as (0.05 %) and left for(14.5, 16.5, 18.5, 20.5, 21.5)for each group. Macroscopic observations on each group were recorded including changes in thyroid gland morphology, hypertrophy and enlargement at each period (14.5, 16.5, 18.5, 20.5, 21.5) days of gestation for both pregnant rats and embryos,the shape of uterine hornes,and uterus with changes of fetus number, resorption, atrophy, malformation of body parts, indifferentiation of fore and hind limbs, skin abnormalities were noticed. The changes in placenta which appeared irregular, pale colour, congested with white fibrous regions were noticed in hypothyroid rat comparing with placenta of control group animal, which looked red, discoid shape and normal surface without any abnormal signs, in addition fatty ovaries with large amount of adipose tissue was accumulated in abdomen cavity, the ovaries irregular and showed structures in most pregnant rats of hypothyroid group comparing with control pregnant females.Present showed significant decreased at (P≤0.05) in mean length, weight and number of fetus at (14.5, 16.5, 18.5, 20.5, 21.5) days of gestation in all embryos related to hypothyroid rats compared to control. Histological study with light microscopy on sections from thyroid gland related to rats with induced hypothyroid showed reduced, small follicles with empty or little colloid substance, each follicle lining simple cuboidal or flat epithelium, an increased with stroma, variable size of follicles most of them smaller compared to control thyroid gland from control rats, which showed normal lobules, large follicles filled with colloid, and poorly stroma.Sections from uterus, ovaries, oviduct related to hypothyroid rats showed variable histological changes ranged from mild to severe like abnormal reduced layers of uterus, irregular uteri folded, degenerated with simple epithelial tissue that lining the uterus villi, haemorrhage in uterine gland, deposition of collagenous fibers, infiltration of lymphocytes and accumulation of adipose tissue. Ovaries from hypothyroid rats showed variable changes with ovaries follicles maturation, accumulated of adipose tissue, decreased graffian follicles, infiltration of lymphocytes and deposition of collagenous fibres with degenerated of lining epithelial layer compared with control ovaries.Microscopic observations of placenta sections from hypothyroid rats at different period of gestation revealed loss tissue, reduced placental layers thickness, necrosis in the peripheral regions, degenerated cytotrophoblasts that surrounding capillaries, the trophoblast giant cells appeared with degenerative changes in labyrinth and spongiotrophoblasts regions. undifferentiated fetal blood capillaries compared to control which the sections have normal structure differentiated to decidual basalis and labyrinth zones, the junctional zone composed of outer giant cells that separated the decidua basalis and trophospongium.In this study the ultrastructural changes in thyroid gland from maternal and fetus related to hypothyroid rat at (14.5, 16.5, 18.5, 20.5, 21.5) days of gestation were identified by electron microscopy and included changes in apical cytoplasm of follicular cells to microvilli, blebs and protrusions also dilated rough endoplasmic reticulum, increased lysosomes, the empty vacuoles more than the dense vesicles, increased mitochondria and Golgi apparatus cisternae, changes chromatin and more cellular debris. Biochemical results in thyroid hormones revealed to significant increase in mean concentration of (TSH) in pregnant females with hypothyroidism during gestation periods in compared with control group, also there was an decrease in both (T3, T4) non significant, significant increased concentration in serum of pregnant rats on treated group with (Ptu) compared with their concentration of control pregnant rats.Levels of some oxidative enzymes (GSH & MDA) was estimated in this study and there was significant increased,non significant in their concentrations in serums of rats with induced hypothyroidism comparid to control group during each period of gestation (14.5, 16.5, 18.5, 20.5, 21.5) day. The study determined some of haematological parameters such as (R.B.Cs) count, (H.b) level and (P.C.V) ratio which showed non significant at (P≤0.05) hypothyroidism rats compared with control rats at the same period of gestation. Also the results revealed non significant with (T.L.C), (D.L.C) in all pregnant rats with hypothyroidism compared to control rats, the data showed an increased with lymphocytes and Neutrophils and Esoinphils cells in smears prepared from hypothyroidism rats compared to control group

بعض الجوانب الحياتية للروبيان الدخيل والروبيان Macrobrachium nipponense (De Haan,1849) من Metapenaeus affinis (H. Milne Edwards, 1837) بعض المواقع المائية في محافظة البصرة == Some Biological aspects of the Invasive Shrimp Macrobrachium nipponense (De Haan,1849) and The Shrimp Metapenaeus affinis (H. Milne Edwards, 1837) from some Aquatic sites of the Basrah province

Author name: انوار مالك جبار المالكي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study intended to shed the light on some biological aspects of the invasive oriental river Shrimp Macrobrachium nipponense compared with that of Jinga shrimp Metapenaeus affinis . For this purpose, Monthly samples were collected and chosen from four Aquatic sites was chosen in the Basrah province (one at each of Al - Mashab and Al - Sallal Marshes and two at Al - Garmah River) during the period from July 2015 to June 2016. collection was done by Fishnet pulled by a boat .The Shrimp numbers were calculated during the time unit (ind./hour) .The Water’s temperature and salinity were studied, it ranged from 15 - 33 ͦ c and 2 - 9.1psu respectively. From a total of 3326, individuals were collected during the study period, only 7% were belong to the first shrimp (M. nipponense ) Compared with 93% to the second shrimp( M. affinis ).The number of each species studied shrimps, varied the monthly. The high number of M. nipponense was 38(ind./hour) recorded during July 2015 and June 2016, while the lowest was 2 (ind./hour) recorded during October and November 2015 . For M. affinis The number of individuals ranged between 980(ind./hour) during August 2015 and 29 (ind./hour) during November 2015 .The comparative monthly percentages were high during seven and six months in the marshes sites recorded for first and second shrimp respectively. The monthly variation in percentages of the two species of shrimps was studied, It ranged (for M. affinis) from 98.8% during August 2015 to 56.8% during July 2015 , while for M. nipponense , It ranged from 43.2% during July 2015 to 1.2% during August 2015 .The results of Frequency showed that the highest percent (100%) was recorded in four months for M. nipponense , these are July, October 2015 February and May 2016. While M. affinis was recorded in most months.Summary bThe population sizes for the two species were studied. For the first species( M. nipponense ), the size class 50 - 60mm dominant during all months of the study period, While for the second species (M. affinis), the size classes 40 - 50, 50 - 60, 60 - 70, and 70 - 80 were recorded during all the months for the first shrimp, the highest percent was 47.3% recorded for the size class 70 - 80 mm , While there is no individuals recorded for the size class 10 - 20 mm during all the months except July 2015 for the second shrimps ,the percentage ranged between 41.4% and less than 1% recorded during May 2016 to March 2016 respectively. On the other hand , the total Frequency for M. nipponense ranged from 83.3% to 0% for the size classes 70 - 80 mm and 10 - 20 mm respectively, While for M. affinis was from 100% recorded for the classes 40 - 50, 50 - 60, 60 - 70 and 70 - 80mm to 8.3% for the class .The Length - Length and Length - weight relationships for the two species were studied The results showed positive correlation between the total length and each of cephalothorax length and abdomen length (r= 0.932 and r= 0.945 respectively, P≤0.01 ) for M. nipponense , Also M. affinis , the correlation was positive for the total length and each of cephalothorax length(r= 0.938) and abdomen length (r= 0.971) (P≤0.01 ) . The correlation was positive between the total length and wet weight for each M. nipponense (r= 0.918 P≤0.01) and M. affinis (r= 0.913 P≤0.01).The Statistical analysis for the weight - weight relationships showed positive correlation between dry and wet weight for M. nipponense (r= 0.966, P≤0.01) and M. affinis (r= 0.918 P≤0.01).The correlation between the lengths of the body and the 2nd chela for male & female of M. nipponense (r= 0.909 and r= 0.823 respectively), these values of correlation refer to the continuous growth of the 2nd chela in male even after the adult stage .Summary cThe concentrations of ten heavy and light metals in shells & muscles of the two shrimps were studied. The results showed a significant differences in eight of them (Cadmium, Potassium, Calcium, Iron, Magnesium, Nickel, Zinc and Sodium) . While there is no significant differences recorded for Lead and Cobalt .The study of some biological aspect which concerned with distribution of eggs on pleopods of females of M. nipponense , showed that the highest mean of eggs was 547.3 (38%) carried on the 2nd pleopod while there are fewer means carried on 1st and 3rd pleopods , and no carry eggs (0%)were carried on the 5th pleopod. The Statistical analysis showed that there was significant differences between the eggs carried on 4th and 5th pleopods and each of the rest ones . lengths of pleopod was positively correlated with the number of eggs (r= 0.661, P≤0.01) The weight of ovigerous female was positively correlated with weight of eggs (r= 0.835, P≤0.01) .The combined effects of salinity and temperature on survival of newly hatched larvae (zoea1 and zoea2) of M. nipponense for 24 & 48 hours’ were tested.The highest survival percentage (100%) for zoea1 was recorded in 12 psu salinity in each of 18, 22 and 26 ͦ c ,While the lowest survival (0%) was recorded in distal water in each 30 and 34 ͦ c . the highest survival percentage was recorded in 12 psu salinity in each 18, 22, 26 and 30 ͦ c. While the lowest one recorded in distal water and 34 ͦ c. The Statistical analysis showed that the percentage survival correlates negatively with temperature and positively with salinity each alone. the combined effect of salinity and temperature on survival was positive with percentage ranged between 38 - 56 % .

عزل وتشخيص بعض الانواع الجرثومية من مياه الانهر الداخلية في مدينة البصرة ودراسة قابليتها على المعالجة الحيوية للعناصر الثقيلة == Isolation and identification bacterial species from inner rivers in Basra governorate and study their ability to Bioremediation of heavy metals

Author name: انوار عبد الوهاب مكي
Supervisor name: علي عبود شريف
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was conducted during November and December 2013.It included studying six inner rivers branching from Shatt al - Arab river in Basrah governorate.These rivers are Abu Al - khasseb, Al - Ribat, Al - Khandaq, Al - Ashar, Al - Khura and Al - Sarraji .The study included isolation and identification of three species of genus Bacillus which are B. licheniformis,B. megaterium and B. badius ,and two species of genus Micrococcus which are M. halobius and M. kristinae .Physical and chemical characteristics of the water samples were measured, which including pH,temperature,electric conductivity and Dissolved oxygen. The results showed that there were not correlation between physical and chemical characteristics and the concentrations of the heavy metals except for water temperature which had a negative significant correlation with the concentrations of nickel and lead.The concentrations of heavy metal dissolved in water (copper, nickel , lead and cadmium ) were measured. The concentrations of Cu+2 and Pb+2 were higher than that of Ni+2 and Cd+2 in the water .However,in general the concentrations of Cu+2, Ni+2, Pb+2 and Cd+2 were high in comparison with the local and international standards.All species of bacteria showed high resistance to the heavy metals ions starting from concentration of 1 mg/l. B. licheniformis was recorded high resistance to Pb+2, Cu+2 and Ni+2 which reached 2600 mg/l, 500mg/l and 300mg/l respectively. M. halobius was also recorded a high resistance to Pb+2 and Cd+2 with 2600mg/l and 400mg/l respectively. The highest resistance was recorded by M. kristinae to nickel with 300 mg/l . The mid resistance was by B.badius to all studied heavy metals and the lowest was by B. megaterium to all heavy metals studied in comparison with the other species of bacteria.The identified bacteria were used in the process of bioremediation of the heavy metals (Cu+2, Ni+2, Pb+2, Cd+2) with different concentrations (25, 50 and100 ) mg / l. B. licheniformis showed high ability to remove Ni+2, Cu+2 and Cd+2 than other species of bacteria, which reached (28.30%, 44.34% and 24.27%) mg/l. . respectively. B. badius showed a high capability for removing Cu+2 with 45.78% mg / l .Where as B. megaterium showed a high capability for removing Pb+2 with 23.43% mg / l.. M. halobius had the highest capability for removing Cd+2 with 25.42% mg / l. and M. kristinae had a mid capability to remove the heavy metals under studied.The study included observing the effect of the incubation period on removing the heavy metals ions.The results found that the best period was 72 hours in comparison with 24 and 48 hours.Also, the study included the effect of initial concentration in the process bioremediation.The results showed that the concentration of 25mg/l was the best for removing the heavy metals ions among the other concentrations, 50 and 100 mg/l.

عزل وتشخيص احد قلويدات الايض الثانوي لبعض الطحالب ودراسة فعاليته الحيوية == Isolation and identification one alkaloid of secondary metabolites from some algae and study of biological activity

Author name: انفال نوري عباس اللفتة
Supervisor name: احمد محسن عذبي | اقبال جاسم الاسدي
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study includes an isolation, Identification and purification of three species of algae, two of them belonging to cyanobacteria they are Oscillatoria brevis and Nostoc carneum . The third one was Enteromorpha intestinalis which belong to green algae which was from different location in Basrah .There are two extract were prepared from the algal species Alcohol , and alkaloid extracts , Alcoholic extract prepared to know what was compound it had and alkaloid extracts to test bioactivity of algae Cytotoxcity also was carried out on human red blood cells, the results revealed that alkaloid extract from E.intestinalis was nontoxic, whereas bioactive compounds isolated from O.brevis , N.carneum. Showed hemolytic action .The bioactivity of alkaloid extract was examined to elucidate their on ability to inhibit the growth of gram Positive and negative bacteria. .Biological activity of alkaloid extracts of three algae isolated were determined by using the Minimal Inhibitory Concentration (MIC) againsttwo bacterial isolates. And also test the effect of filtrate pure algal culture on growth of plant pathogenic fungi ,also apparently that the algal alkaloid extract and isolated compounds exhibits antioxidant.The antitumor activity of the algal alkaloid extract from E.intestinalis against Rhabdo myosarcoma cell line was examined. The results showed that crude alkaloid extract possessed an antitumor bioactivity at low concentration 0.78mg / ml .In this study also was carried out to investigate the possibility of preventing the hypercholesterolemia by using alkaloid extract of E.intestinalis .it also aimed to study the effect of use this extract in reducing the Plasma Total Cholesterol (TC) , Triglyceride (TG), Low - Density Lipoprotein (LDL) , Very Low - Density Lipoprotein (VLDL) and no effect on High - Density Lipoprotein (HDL).The identification of the compound was made depending on the active groups test and spectroscopic analyses including : Infrared (IR) and Gas Chromatography / Mass spectrum (GC - MS). The results of such analyses showed that alkaloid extract from E.intestinalisa has three compound these are Hexadecanamide , Methenamine and Benzenedicarboxylic acid, diisooctyl est and alkaloid extract from N.carneum showed that has three compound 1,2 - Benzenedicarboxylic acid diisooctyles,ا9 - Octadecenamide, (Z) - )ااااا and Didecyl phthalate .Hexadecanamide purification from alkaloid extract of E.intestinalis depended on physical and chemical properties The identification of the compound was made depending on the active groups test and spectroscopic analyses including : Infrared (IR); Gas Chromatography / Mass spectrum (GC - MS) and this component showed bioactivity on gram. Positive and negative bacteria and it has also antioxidant activity

استخدام عوامل استحثاث لانتاج مؤيضات ثانوية من بعض عزلات انواع الفطر واختبار فعاليتها الضد بكتيرية والخلايا السرطانية Penicillium والكولسترول == Use of inducing agents in secondary metabolites production from some Penicillium isolates and assay their activity against bacteria , cancer cells and cholesterol

Author name: امل صالح عبد الرضا
Supervisor name: توفيق محمد محسن
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study was designed to investigate active compounds from different Penicillium isolates ,that isolated from different sources , using different media . Eleven isolates related to five species : P. chrysogenum, P.paxilli , P. citrinum, P. cosmopolitanum, P. digitatum were isolated. After primary screening was performed , the best isolates that have high antibacterial activity against gram positive and gram negative bacterial was selected. The selected isolates were cultured on fermentation media according to the suitable media for each isolate, and the active compounds were extracted using ethyl acetate as a solvent. P. cosmopolitanum and P. chrysogenum were cultured on penicillin production medium (PPM) , P. paxilli was cultured on Aspergillus complete medium (ACM) and P. digitatum and P. citrinum were cultured on solid state fermentation media consist of wheat bran . The efficiency of fungal crude extracts from each isolate against the reference strains S. aureus NCTC 6571 and E. coli ATCC 25922 and against Candida albicans (pathogenic and reference strain)was examined, and the Minimum inhibitory concentration (MIC) was determinated for each extracts.The inhibition zone of fungal extracts against E. coli and S. aureus ranged between (20 - 50) mm , and they had high activity against C. albicans ( reference and pathogenic isolate) ranged between 25 - 45 mm.Induction of secondary metabolite production by the selected fungi was carried out using biological factor (Streptomyces sp.) , chemical agent (colchicine) and physical agent by UV radiation. Apparently that the biological induction by Streptomyces isolate was the best compared with the other treatments.The results revealed that the two fungal extracts ALO1 and Apax exhibit a high antioxidant activity at low concentrations and volumes of the extract compared to ascorbic acid as control. ALO1 had radical scavenging activity (RSA%) reach 99.9% at volume 3μl , and Apax had RSA 91.6% at concentration 500μg/ml. Also, the fungal extract ,ALO1, showed a significant activity as anticancer agent against RD - cell line. The IC50 of ALO1 was 8.5μl after 24h and 23.76μl after 72h. The hypercholesterolemia ability of ALO1 in vivo and in vitro was assayed . In vivo experiment designed using female albino mice and divided into three groups each one contain 8 mice : group 1 treated with 10μl , group 2 treated with 20μl of ALO1 , and group 3 treated with normal saline as control group. The results showed that total cholesterol , triglycerides, LDL and VLDL were significantly reduced when compared with control group while the HDL level was increased in comparing with control group. The chemical analysis of the crude extracts showed that Apen2 contained 259 μg/g of penicillin G when analyzed with HPLC apparatus . Also the GC mass analysis of ALO1 showed that Docasadionic acid initialize area76.18% and Palmitic acid initialize area 15.09% from the total identified compounds.Whereas the GC mass analysis of Apax showed that Hexandioic acid initialize area40% and Stearolic acid initialize area 35.57% from the total identified compounds.In addition , it was detected the presence of penicillin biosynthetic gene cluster : pcbAB, pcbC and penDE in P. cosmopolitanum and P. chrysogenum.The result showed that gene pcbC and penDE were found in the genomic DNA but the gene pcbAB was not amplified by PCR.
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