عزل وتشخيص بعض انواع من بكتريا المايكوبلازما من عينات سريرية في مدينة البصرة == Isolation and identification of some Mycoplasma species from clinical samples in Basrah city
Author name:
رواء صادق مجيد
Supervisor name:
غيداء جاسم عبد النبي الغزاوي
General topic:
Biology
Specific topic:
Life Science
Degree:
Master
University:
University Of Basrah - College Of Science - Department Of Biology
Language:
Arabic
University location:
Basrah
First pages:
24T3540 - p.pdf
Abstract:
In the present study , a total of 150 clinical samples were collected (50 samples of sputum ,50 swabs of gingiva and 50 vaginal swabs ) from patients visited Basrah general hospital and from the 1st specialized center of dental medicine in the city of Basrah . This study extended from January 2015 to May 2015. Samples were taken from males and females (except vaginal swabs where were collected from females only) .Their ages ranged from 6 - 70 years old. These samples were collected and cultured in a method monophasic - diphasic culture setup ( MDCS ) . Three types of Mycoplasma were isolated : Mycoplasma pneumoniae from sputum , Mycoplasma salivarium from gingival swabs and Ureaplasma urealyticum from vaginal swabs .These isolates diagnosed by biochemical tests and PCR. Mycoplasma spp. were isolated from 76 individuals out of 150 enrolled in this study, infection rate was 50.5% , 25 cases were diagnosed as M. pneumoniae (50%) , 13 cases were diagnosed as M. salivarium (26%) and 38 cases were diagnosed as U. urealyticum (76%).Mycoplasma was studied in related with two factors : sex and age . In this study, the Mycoplasma medium is modified , egg yolk was used instead of horse serum as source of cholesterol and also used two amino acids Arginine and Cysteine as well as urea ( when diagnosing U. urealyticum ) to support Mycoplasma growth. Besides that , thallium acetate was replaced by sodium benzoate , finally , magnesium sulfate was used as a sign of ammonia when diagnosing U. urealyticum.In this study , PCR technique used to diagnose species of Mycoplasma using ATPase gene ,16s rRNA gene and Urease gene .Sixty - eight isolates were identified by PCR technique , 25 of them were M. pneumoniae , 13 were M. salivarium and 32 were U. urealyticum.
