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دراسة بيئية لبعض العناصر الثقيلة في مياه نهر الفرات عند مدينة الناصرية وقابلية مراكمتها من قبل بعض الطحالب الخضر المزرقة ونبات الشمبلان مختبريا == Ecological Study of Some Heavy Metals on The Euphrates river Water at Nassiriyah City and The Ability of Accumulation by Some Blue green Algae and Ceratophyllum demersum L. in the Laboratory

Author name: عبد الوهاب ريسان عيال العبادي
Supervisor name: احمد محسن عذبي | اقبال جاسم الاسدي
General topic: Biology
Specific topic: plant - aquatic life
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The diversity of phytoplankton were studied and some physical and chemical environmental factors are (Temperature of air and water , pH , electrical conductivity , salinity , dissolved oxygen , alkalinity , hardness and nutrients) of four locations on Euphrates river water at Nassiriyah city in Thi Qar governorate . The first location lies north - west of Nassiriyah city at Al - Sharif region , whereas the second location lies in Al - Mustafaweia region , and the third location lies in Al - Majzarah region , and finally the fourth location lies in Al - Fadlyia city . Samples of water were collected from January to December of the same year of 2014 .The concentrations of some heavy metals are (lead , cobalt and cadmium ) were estimated , in addition to limitation of the efficiency of some algal species and Ceratophyllum demersum L. in bioremediation of their elements on the river water and a limitation of the accumulation sites of the heavy metals on the cells of the algal and plant by using the scanning electron microscope - x - ray . The results revealed clearly a variation on monthly , quarterly and locations of the number properties of the physical and chemical that were studied , the temperature of water ranged between 11.5cہ - 43.6c ہ and air ranged between 15.5cہ - 44.9 cہ , the high temperature at the second location was recorded .The high value of pH 8.62 in the forth locations at April was recorded and the low 7.2 in the third location at July . The concentrations of electrical conductivity were ranged between 3308 - 5773 μs / cm , whereas the concentrations of salinity ranged between 2.11 - 3.69 gm / l , the high concentration on the third location at February was recorded , and the low on the first location at April was recorded , while the concentrations of the dissolved oxygen ranged between 5.40 - 11.70 mg / l , the high concentration on the first location at February was recorded , whereas the low concentration on the third location at August was recorded , also the alkalinity concentrations ranged between 13 - 250 mg / l , the high concentration on the fourth location at March and the low concentration on the third location at July was recorded . From the results , it was show that , the river water was hardship , the hardness concentrations ranged between 850 - 1610 mg / l , the high concentrationaon the third location at January , and the low on the second location at May was recorded .While the concentrations of the nutrients , as nitrate and phosphate , the nitrate concentrations ranged between 13.6 - 41.5 mg / l , the high concentration on the third location at February and the low concentration on the first location at July , whereas the phosphates concentration ranged between 0.83 - 1092 mg / l , the high concentration on the third location on first location at April.Also the diversity of phytoplankton had been studied that were present in water of the river, There were diagnosis 198 species distributed between six classes , the dominance to the Bacillariophyceae as 90 species which belonged to 19 genus were recorded and at the percentage 45.45% of the total numbers of species recorded , followed by the Chlorophyceae 46 species which belonged to 25 genus and at the percentage 23.23% of the total species were that were recorded , followed by Cyanophyceae algae 46 species which were belonged to 15 genus and at the percentage 23.23% and algae of the Euglenophyceae had eight species which were belonged to three genus record and at the percentage 4.04% , with regard to the algae of Xanthophyceae had recorded five types at there belonged to three genus and at the percentage 2.52% and finally the algae of Chrysophyceae which was recorded three types belonged to the same genus , which collectively represent the percentage 1.51% .The study also experienced a record for species of phytoplankton at the first time in Iraq , which were five belonged to four classes , one of them was belonged to Chlorophyceae algae (Trochiscia granulate Prescott.) and the others to the Euglenophyceae(Phacus pyrum (Ehrenb.) Stein and Trachelomonas simillis Stokes) and one species for Chrysophyceae(Mallomonas elliptica (Kisselew) Conrad)and Xanthophyceae algae (Peroniella planctonica G.M.Smith).The concentration of some heavy metals (lead , cobalt and cadmium) in water of the locations was studied by using the flame atomic absorption spectrophotometer , the results were showing that , the concentrations of lead ranged between 4.58 - 29.0 μg / l , as record the high concentration on third location at August and the low on the first location at December , while the concentrations of cobalt were ranged between 4.60 - 23.0 μg / l , as record low concentration was on the first location at December ,bwhile the high concentration on the third location at August , cadmium concentrations ranged between 0.84 - 14.63 μg / l , the low concentration 0.84 μg / l recorded in the first location at December , while the high concentration 14.63 μg/ l in the third location at June .The study included too . a laboratory experiments to demonstrates the efficiency of some species of algae and Ceratophyllum demersum L. on the bioremediation of pollutants in the river water . The results indicates that , there was an increase on the percentage of the removal of elements with the increasing concentrations of elements that were added to water aquarium , reaching the low percentage of biological remove of the lead 95.2% at the treatment 50 mg / l and at the time period 10 days , whereas the biological remove of the high percentage 99.2% at the same treatment during the time period 40 days.As for the cobalt element , the results showing that , there were a gradual increases of the proportion of the removal with the increasing concentrations of cobalt that were added to the water aquarium , and reached to 94.2% at the treatment 50 mg / l at the time period 10 days , whereas the percent for the removal 98.56% at the time period 40 days at the same treatment , whereas the percentage of the vital removing of the element cadmium by the water plant Ceratophyllum demersum was increases in removing from the water aquarium that was 92.6% at the treatment 50 mg / l at the time period 10days , whereas it was 97.40% at the same treatment .As for the algae it had been isolated and diagnoses , the were four species of , blue - greens (Microcystis aeruginosa Kütz. , Nostoc linckia(Roth) Bornet and Thuret , Oscillatoria amoena(ktz.) Gomont and Hapalosiphon aureus West and West , samples were collected from the Euphrates river water on the studied locations . It was purified and propagation of these species on the laboratory for a single culture cell , unialgal culture and axenic culture to test their ability to remove and accumulation of the heavy metals from water. These species of algae were treated salt solution of heavy metals are lead , cobalt and cadmium both in privates and at different concentrations 0.10 , 0.25 , 0.50 , 1.00 and 2.00 mg / l for a period of two weeks . H.aureus was accumulated more of lead 1.724 μg / gm at the treatment 2.00 mg / l , followed by O.amoena which accumulated 1.638 μg / gm and N.linckia accumulatedc1.621 μg / gm and finally M.aeruginosa accumulated 1.579 μg / gm at the same treatment , but when adding the salt solution of cobalt nitrate , the alga O.amoena was accumulated more of cobalt element 1.726 μg / gm at the treatment is 2.00 mg / l , followed by H.aureus as accumulated 1.638 μg / gm , and was N.linckia accumulated 1.535 μg / gm , followed by M.aeruginosa 1.428 μg / gm at the same treatment . When are using the solution of the salt cadmium acetate , the N.linckia was accumulated more of cadmium 1.08 μg / gm , followed by H.aureus which was accumulated 0.63 μg / gm and finally O.amoena accumulated the percentage was reached 0.59 μg / gm at the same treatment .The study was included too the a limitation sites and accumulation of heavy metals within the plant cells and algae that were studied by using technology of scanning electron microscope - x - ray and the use of reagent X - ray EDX with SE2 .The results were showing that , The locations which accumulated heavy elements was in the cytoplasm of the cell . On the above of the foregoing the Ceratophyllum demersum L. showing high efficiency of accumulation of heavy elements inside the cells as compared with the studied algae , the efficiency and ability to increase of the accumulation increases by added of elements and by the increases period of exposure , also algae were revealed a capabilit

حساسية وخصوصية تقنية تفاعل السلسلة المتبلمرة Polymerase Chain Reaction PCR وبعض الطرق المصلية في الكشف عن الاصابت بطفيلي Toxoplasma gondii في النساء المجهضات وازواجهن == Sensitivity and specificity of Polymerase Chain Reaction (PCR) Technique and some Serological methods in the detection of the infection with parasite Toxoplasma gondii in women with recurrent miscarriage and their husban

Author name: فيحاء نوري عبد الحسن
Supervisor name: صبيح هليل المياح | شيماء جبار ريسان
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: Atotal of 300 blood samples were collected from abortifacients women and their husbands who consalted Al Sadr Teaching Hospital in Maysan province and private laboratories in Basra during the period from July 2015 to October 2015, In the same time 50 samples of uninfected males and femals were collected and used as a control groupAtotal of 300 blood samples were collected from abortifacients women and their husbands who consalted Al Sadr Teaching Hospital in Maysan province and private laboratories in Basra during the period from July 2015 to October 2015, In the same time 50 samples of uninfected males and femals were collected and used as a control group . The examination by Enzyme Linked Fluorescent Assay (ELFA) demonstrated that 160 (53.3%) of these samples were positive for toxoplasmosis , at rate of 53.3% , IgG antibody recorded an increase in percentage comparing with IgM and IgG + IgM together , IgG antibody recorded increase in the 138 samples at rate % In PCR technique two genes B1 and Tghsp70 were used to diagnose the same samples for the first time in Iraq and showed highest sensitivity (90.7%) . However age group 31 - 35 years recorded a high percentage of infection (90.5%) . The sensitivity of LAT test for detection of the toxoplasmosis was 80% , ELFA test recorded sensitivity 53.3% and PCR 90.7% with specificity were 60%, 53,3% and 70.7% respectively . Also the concentration of heat shock protein HSP70 in abortifacients of women and their husbands has been estimated , according to concentrations of 20, 40, 80, 160 and 320(ng/ml).II SummaryWhen examined used by ( LAT) test the results showed that males differ fromfemales with a significant difference P≤0.05 , the rate of males were 70% in theconcentration 160ng/ml , But it was observed that women with recurrent abortion, had higher rate 30.8% with high significant difference at the level of probabilityP≤0.01 in the concentration 80ng/ml , compared with males . The results of theage groups showed had highest levels of heat shock protein HSP70 were in theage group (>40) years at rate 66.7% in a concentration 320ng/ml and with highsignificant difference P≤0.01 compared with other age groups, While the agegroup (20 - 25) years had higher rate 11.4% in concentration 20ng/ml comparedwith other age groups , also the age group (26 - 30) years had higher rate 23.3%and 56.7% in concentrations of 80ng/ml and 160ng/ml respectively comparedwith other age groups.The results of estimated heat shock protein for the samples examined by ELFAtest showed that husbands were different from their wives and had higherrate75% with high significant difference P≤0.01 in concentration 160ng/ml .While the ratio of females were 37.5% in the concentration 320ng/ml higher thanthe males with a significant difference P≤0.05 , Also the result showed highsignificant difference P≤0.01 in concentration 160ng/ml and 320ng/ml betweenthe age groups , the age group (35 - 31) years had higher rate 66.7% inconcentration 160ng/ml and the age group (>40) years in concentration320ng/ml had higher rate 66.7%.As well the results of estimated heat shock protein HSP70 of the samplestested by PCR assay in the current study showed a significant difference P≤0.05between males and females , while the ratio of males at rate 59.4% higher thanthe ratio of women in a concentration 160ng/ml , but the rate of women understudy in concentrations 40ng/ml and 80ng/ml were at rate 11.8% and 27.2% ,respectively higher than the males with high significant difference P≤0.0 , onthe other hand no significant difference showed in this study between males andfemales in concentrations 20ng/ml and 320ng/ml , but the results showed thatage group (>40) years had higher rate 66.7% in the concentration 160ng/mlcompared with other age groups . While the age groups (20 - 25) years had higherrate 22.9% in concentration 80ng/ml compared with other age groups . So theage group (36 - 40) years had higher rate 14.3% in concentration 20ng/mlcompared with other age groups .Ministry of Higher Education University of Basrah Sensitivity and specificity of Polymerase Chain Reaction (PCR) Technique and some

دراسة الطفرات الوراثية في جيني TPO وTSHR في محافظة البصرة

Author name: فله عبد الستار
Supervisor name: فائزة عبد الوهاب احمد | اسعد يحيى عايد
General topic: Biology
Specific topic: Plant - Genetics
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: Considered the thyroid glands task Deaf which produces important hormones are Tri iodothyrosine T3 and Thyroxin T4 and is responsible for their production a number of genes that encode the enzymes and proteins and the occurrence of any defect in this gene lead to the emergence of disorders of the thyroid work, including the case of palaces and fragments in hyperthyroid through the emergence of different pathological symptoms that indicate the occurrence of one of the cases studied in this research gland disorders at the level of molecular genetics .The study included only females were collected blood samples from 70 patients divided into 35 blood samples of the state of palaces and 35 blood samples for the case of the bulk in hyperthyroid deaf and diabetes endocrine centers of hospitals and ports Fayhaa year and Qurna, as well as from laboratory d. Nizar Mahfouz and represented a group of patients and 20 blood samples from healthy people represented as a set of control and that during the period from April to September 2013. But has been working on a sample of 70 patients (35 blood samples by the state of palaces and 35 blood samples for the case of the bulk gland) to add to the 20 blood samples of control and ages of the two groups and the healthy range (12 - 60 years).DNA was extracted DNA of two and three encoded amplified regions (8, 9 and 14) for the TPO gene and encoded region tenth TSHR gene PCR technology after it was diagnosed and analysis of mutations using the method for Sequences .The results showed the presence of two types of changes in the TPO and TSHR genes , two polymorphism (SNP) and Mutation, in gene156TPO study found hereditary forms c.1117G> T, A372S and c.1194G> C, S398T in the two groups of patients and healthy and not they relate to the occurrence patients palaces gland activity, as the study diagnosed 12 Novel Mutation in exon 8 in 21 patients hypothyroidism as compared to control (who did not appear to have any kind of mutations diagnosed) and that all mutations diagnosed are mutations bitmap compensatory (substitution) the type of guarantee or Transtion Transversion depending on the nature of the base mutant, five of the mutations are Missense Mutation in 11 infected condition limitations are as follows c.949A>G,M317V , c.1064G>A,R355H, c.1277C>G,A425G , c.1063C>A,R355S and c.1207C>A,L402M and seven silent mutation in 10 patients hypothyroidism c.1062G>A,A354A , c.1050C>A,L350L , c.843C>A,A281A , c.1101G>A,V367V, c.1143C>T,I381I , c.1071G>A,R357R and c.2406C>T,D802D , While the results did not show any difference in the analysis of the genetic sequence of the two exons 9 and 14 at comparable with the genetic sequence of the control group. In this study suggests that there may be a correlation between the occurrence of mutations in a gene TPO and the state hypothyroidism . The results of the current study showed that the gene TSHR has an impact in both cases, and shortcomings in fragments in the activity of the gland where the study found three types of polymorphism (SNP) of the two of them Non - Synonymous type c.2181G> C, E727D and c.1489G> C, A496P and shape The last of the silent type genetic c.1377G> A, A459A, as the results showed that the two forms hereditary c.2181G> C and c.1489G> A have no correlation to happen disorders and are globally registered the shape genetic c.1489G> C, A496P did not score previously has current study for the first time and may have a link to happen one of the disorders depending on the genetic predisposition of the individual . I found the157current study, seven Novel Mutation in exon 10 distributed to five mutations among patients hypothyroidism as compared to control (who did not appear to have any kind of mutations diagnosed) and that all the mutations diagnosed are mutations bitmap compensatory (substitutive Substitution) to ensure the kind of Transtion or Transversion depending on the nature of the base mutant 'three mutations are Missense Mutation in 3 people with the status of the palaces and the proportion of each mutation them (20%) are as follows c.1330 T>C , Y444H ; c.1424 T>C ,L475P and c.1435 T>C , S479P and two silent mutation in patients hypothyriodism c.1338 G>A, L446L and c.2300 G>A , Untranscrib , While the results recorded and there is only Tafrtin in the case of the bulk in hyperthyroid by (5.71%), one Missense mutation (c.1832 C> A, P610Q) and the other is silent (Silent) (c.2103 C> A, R701R). The present study indicated that the probability of the occurrence of the disease mutations link through the site mutations in specific areas to link hormone catalyst and the interaction between the Receptor and the unity of secondary alpha (G - protein). Results of the current study also showed that mutations identical and disparate factors have an impact on events in the case of deficiencies, genetic gland activity in the TPO and TSHR through defect events and constructed partially or wholly in the function of the protein encoded by genes TPO and TSHR

عزل وتشخيص مركب ايضي ثانوي جديد لكل من الفطرين Fusarium Trichoderma harzianum وsolani المعزولين من السواحل الرسوبية في محافظة البصرة وتقييم فعاليتهما الحياتية == Isolation and Identification of Novel Metabolic Compound From Both Fusarium solani and Trichoderma harzianum Isolated From Sedimentary Coast of Al - Basrah City and Estimate their Activity

Author name: فردوس نوري جعفر
Supervisor name: محمد لطيف علي | نيران جاسم الصالحي
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: with the interest of medical and pharmaceutical uses of the natural products from marine fungi , thirty species of fungi belong to ninteen genus were isolated and identified from sea coast sediment of Al - Faw and Al - Seeba .The fungi Acremonium strictium , Geotricum candidum, Fusarium solani ,F.oxysporum , Penicilium sp. ,Trichoderma harzianum exhibited high inhibition activity toward growth of two reference strains of bacteria Escherichia coli (ATCC25922) and Staphylococcus aureus (NCtC 6571). The six selected fungi were grown in liquid culture media PDB at 25°C for two weeks ethyl acetate was used to extract the active compounds from thefermentation liquid media while ethanol was used to extract the active compounds from fungal mycelia .The crud extracts (fermentation and mycelium ) showed different inhibition activity against the pathogenic bacteria , Proteus mirabilis , S. aureus, isolates E. coli, Pseudomonas aeruginosa Klebsella pneumoniae. and pathoginic yeastes Candida albicans, C.tropicals,Cryptococcus neoformans and dermatophytic fungi Trichophyton verrucosum and Microsporium canis . The results revealed that the fermental fungi extracts has more inhibition activity than mycelial fungal extracts.The minimal inhibitory concentration (MIC) of fungal crude extracts were determined against gram positive and gram negative bacteria and pathogenic yeasts and fungi . antagonisum between studied fungi were evaluated , and the fungus T.harzianum has been the more antagonistic effect against other fungi. The toxicity of the fungal crude extracts were tested by using of human RBC at different concentration the result of this experiment showed that all extracts are non toxic except F.oxysporum extract.From all the above results the both F.solni and T.harzianum extracts showed higher activities ,thus these two extracts chosen for further studies. The anticancer activity of both extracts were studied against Hela cell line and the anticancer activity appeared at 250 Mg/ml and 62.5Mg/ml respectivily. The antioxidant activity of extracts were examined by three different methods ,B - carotene ,hydrogen peroxide scavenger and ferrus ion reduction , the results of three methods showed that both fungal extracts have antioxidant behaviours and the T.harzianum extract had more antioxident activity than F.solani extract and the antioxidant activity of both fungal extracts were increased as the concentration of the extracts increase.An optimization of the fungal growth and bioactivity of both fungi were conducted, by using different Nitrogen sources ( Asparagine , Yeast extract , peptone , Ammonium chloride , Sodium nitrate , Ammonium sulphate ) and carbon sources ( Maltose , Galactose , Glucose , Dextrose , Sucrose ,xylose and Starch ) the results elucidated that yeast extract was the best for growth of both fungi on solid and liquid media and high inhibition activity appear with T.harzianum against bacteria isolates , the carbon source starchenriched the growth of F.solani with more activity in inhibition the bacterial growth while the sucrose give high enrichment to the growth of T.harzianum in solid and liquid media with high activity toward bacterial growth. The study also indicates that the beast temperature and pH which gives the higher activity and growth 25°C and pH6 . The effect of different concentration of salinity were studied as well (1%,2%,3%,4%,5%,6% NaCl)and 2% showed the best concentration .The activity of two studied fungal extracts were superior than commercial antibiotic toward bacterial isolates .Preliminary qualitative chemical tests were carried out on the extracts and their chemical nature were identified .TLC technique were used and the chemical analysis showed that F.solani extract consist of two components with constant Rf values, the components marked as A1 and A2 . A1 shows higher activity against bacterial strains while T.harzianum extract consist of one component.The chemical structures of the isolated compound were

التشخيص المظهري والتغاير الوراثي لذباب القرعيات Dacus frontalis Becker ,1922 في محافظتي البصرة وميسان == Morphological identification and genetic variation of cucurbit fly Dacus frontalis Becker ,1922 in Basrah and Missan provinces

Author name: فاطمة قاسم حمدان
Supervisor name: فائزة عبد الوهاب احمد
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study is identified (morphologic and molecular )for the population of cucurbit fly which is a destructive pest of cucumber ,honey melon and cantaloupe in Basrah and Missan .Sample were collected from different region from Basrah province including Qurna , Safwan , Zubair , Khor Al - Zubair . Al - Misharah , Kumate , Kalat Salih, Ali Al - Garbi in Missan province for the period October ,2014 till September 2015.Result of the identification study showed there is only one species infect cucurbits which is Dacus frontalis Becker ,1922,family Tephritidae ,Order Diptera in all studied regions. This species was classified according to femur color of the middle leg ,its color is orange at the apical half with dark black spot at the basal part of the antenna with the presence of basal diagonal line underneath the scutum behind the thorax. female were characterized by the presence of ovipositor at the end of the abdomen and male characterized by present s one line of hair at the third of the abdomen ring .For the sensitivity of the insect fly cucurbits towards our fruits of cucurbits (cucumber and water melon represented by the number of eggs laid down by the female as it appeared no significant difference in the number of eggs laid down by the female in the provinces of Basra, Maysan and was the highest rate of lay eggs on the fruits of cucumbers choice of six eggs and a difference of moral on water option, which amounted to one egg.Molecular study show the kit equipped Bioneer company and named Genomic DNA Extraction kit was highly efficient in DNA Extraction it was later used in the Random Amplified Polymorphic DNA - Polymerase Chain Reaction which is one of the methods used in molecular identification .Used four primers for different sequence s. and electrophoresis results shows multiplied bands of different molecular weight for four different primers for both females and males .The number and molecular weight of bands was calculated manually comparative with DNA marker or ladder then used the number of bands in the following equation to find the genetic similarity and genetic distance between the different districts each primer for males and females .To find genetic similarity used the equation : Sfy=2Nfy/Nf+ Ny and to findgenetic distance used the equation : GD fy=1 - SfyAt the level of first primer for females it was less value of genetic distance is zero and this is evidence that one species and highest value of genetic distance is 0.9 on the second level of the primer for females it was less value of genetic distance is 0.2 and highest value of genetic distance is one at the third primer for females it was less value of genetic distance is 0.3 and highest value of genetic distance is one but the fourth primer for females it was less value of genetic distance is 0.3 and highest value of genetic distance is one .For males at the level of first primer of males it was less value of genetic distance is zero and highest value is 0.8 but the second primer of males it was the less value for genetic distance is 0.8 and on the level of genetic distance is zero on the level of third primer for males it was value of genetic distance is zero and highest value is 0.8 and fourth of males it was the less value of genetic distance is zero and highest value of genetic distance is 0.8

دراسة تصنيفية وبيئية لبعض انواع عائلة ذباب الخيل Diptera : Tabanidae ودورها في نقل طفيلي Trypanosoma evansi في محافظة البصرة

Author name: علاء ناظم حاتم علي
Supervisor name: ضياء خليف كريم
General topic: Biology
Specific topic: Zoology - Insects
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:

دراسة الطفيليات الداخلية والخارجية في وعلى انواع مختلفة من الحشرات

Author name: علا عبد الشهيد ناصر
Supervisor name: كاظم صالح حسن الهدلك | مسلم عبد الرحمن الطعمة
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: Present study was carried out to isolate and identify the worms nematodes found in the soil, and could be used as a means of biological control against insects, it has also been to isolate and identify Mites present on or within different types of economic and other insects, which were represented by the American cockroaches Periplaneta americana ( Dictyoptera; Blattiidae). And houseflies Musca domestica (Diptera : Muscidae), Persian and ants belonging to the family; Formcidae) Hymenoptera), flour beetles Tribolium castaneum (Tenebrionidae; Coleoptera), and the termites Microcerotermes diversus( Isoptera; Kalotermitidae))The study samples (soil and insects)were collected from different regions and ecosystem of the province of Basra, during the period of the month of December 2014 to the month of September 2015. By setting traps, appropriate and specifically designed for this purpose, that supplied with special food to attract nematodes in the soil and catch insects found in those ecosystem, . and also to determine the best ways to isolate samples preserved and propagate them..Also, the study dealt with experiments for the propagation of the mites (which collect from insect samples) in the laboratory,by growth them on different growth media, for the purpose of immunological and genetic study, and the ability to be used in bio resistance and also to determine the sensitivity of the laboratory mice, that attacked by the mites through conducting hitological study of the skin of these animals..Results recorded two types of endo parasitic nematodes these areFamily : Steinernematidae1 - Steinernema feltiae2 - S. carpocapsaeAnd five species of mites, these areFamily : Histiomatidae2 - Histiostoma sp1 Family : Acaridae 1 - Cosmoglyphus barbisetusgrifolapholiotae 2 - Mycetosancassania Family : Macrochelidae Vitzthum, 1930 Macrocheles muscaedomesticae Family : Pyemotidaepymephorus tarsalis

تاثير بعض مستخلصات الطحلب الاخضر Chara vulgaris L. على البيوض والطور اليرقي لشريطية القطط Taenia taeniaeformis Batsch, 1786 == The Effect of Some Green Algae Extracts Chara vulgaris L. On The Eggs and The Cysticercus of Taenia taeniaeformis Batsch, 1786

Author name: عقیل عبد الصاحب عبد الحسین الوائلي
Supervisor name: باسم ھاشم عبد الله | عماد یوسف عواد
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:

التغيرات الكيميوحيوية المصاحبة للشد الملحي وتقييم دور مستخلص الطحلب الاخضر Cladophora crispata في نبات الرز Oryza sativa L. المستزرع نسيجيا

Author name: عبد الامير رحيم عبيد
Supervisor name: حسيي خلف زاير | لوى حسين عبد القادر
General topic: Biology
Specific topic: Plant - Physiology
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted at the tissue culture laboratory at the Dept. of biology, College of Education pure Sciences from 1/3/2012 - 1/12/2014.With the objective of studing the tolerance of the salt stress of two rice (Oryza sativa L.)cultivars namely Anber and Furat using the technology of tissue culture. . Mature seeds (mature embryo and endosperm) were used as bing parts of explants which were cultivated in a semi - sold( Murashige and Skoog 1962) nutrient medium and some additives such as vitamins and amino acids .The growth regulator Dichlorophenoxy acetic acid (2.4 - D)was used, about 8mg/l , sucrose at 30g.l - 1 . . The study ,also ,tested the effect maltose at 30g.l - 1, and the effect of 0.5g/l of casein hydrolest were tested.the study also included the different of sea water concentrations (0,20,40,60)%on the induction of growth and development of callus ,the effect of green algae extract at Cladophora crispate by adding 1g fresh weight to the nutrient medium with all sea water concentrations.All the features of the callus were tested as well as the effect of the interaction between sea water concentrations and algae extract , as well as interactions among rice cultivar x sea water concentration x algal extract.The study depended on some growth indicators to determine the degree of callus to tolerance towards salt stress .the resistance of callus to salt depended on regeneration through the required of time for the formation of embryos ,number of germinated embryos ,and number of plantlets .The concentration of the major metal ion : sodium, potassium, phosphorus, calcium ,magnesium, potassium/sodium percentage , and the minor metal ions : iron ,copper, manganese were also some chemical components such as protines and amino acid proline measured, were also measured .The randomized block design (C.R.D)and factorial experiments were used depending ten replicates per treatment .The results can be summarized as follows : 1 - The results showed that the addition of sugar di maltose as an energy source and Casein hydrolyses to the nutrient medium had appositive effect on significant decrease in the required time to mitiate the callus ,in the increase of callus induction percentage and in the increase of freshweight of initial callus. .2 - The effect of sea water and algae extract : . A - The current study shaued that the addidtion of 20 and 40 % sea water to the nutrient medium resulted in a significant increase in the required time to induce the callus ,and a significant decrease in the percentage of callus induction and weight of the mitial callus as compared with the control treatment .The concentration 60%0f sea water caused the mature seeds to lose their ability to germinate and induce the callus if no induction occurs at all . The effect of algae extract resulted in a significant decrease in the required time for callus induction ,and a significant increase in the percentage of callus induction ,the weight of the mitial callus and 0,20,40% of sea water as compared with free algae extract medium.B - The results showed that the addition of 20% of sea water to the nutrient medium that is prepared for the growth of the embryo callus led to a significant increase in the fresh weight as compared with the control treatment , whereas the two concentrations 40 and 60%sea water led to a significant decrease in the embryo callus weight .The effect of the algae extracted to a significant increase in the fresh weight of the embryo callus with sea water concentrations as compared with the free algae extract medium.C - The addition of 20,40 and 60%sea water cause a significant decrease in the number of the somatic embryos ,thir germination auerage ,number and germination of the formed plantlets . This addition also caused a significant increase in the required time to stare the germination of the somatic embryos as compared with the control treatment .The addition of the algae extract led to increase the number of formed embryos ,germination percentage ,and number and percentage of plantlets ,wher was the addition led to lower the required time to germination the embryos compared with the free algae extract medium.D - The addition of 20 ,40 and 60% sea water to the nutrient medium resulted in a significant increase in the concentration of sodium ,calcium ,and magnesium ion in the embryo callus ,while a significant decrease occurred in the concentration of potassium and phosphorus ion and thedpotassium/sodium percentage as compared with the control treatment .The abone mentioned concentrations 0f sea water led to significant increase in concentration of ion ,copper, and zinc ions ,and significant decrease in manganese ion ,The addition of the algae extract caused in lowering the concentration of sodium ion .This decrease was significant in the 60 % sea water medium. The ingrease of potassium ,calcium ,phosphorus, and magnesium ion in embryo callus tissue when compared with free algae extract medium and was significant with some sea water concentration.E - The results ,also ,showed that the addition on 20, 40 and 60% concentrations0f sea water resoled in a significant decrease in concentration of some chemical ingredients such as protein and amino acid proline of the embryo callus in comparison with the control treatment . as far as the effect of the algae extract is concerned ,the addition of this extract to the nutrient medium led to a significant increase in the concentration of the proteins and amino acid prolne as well as with all sea water concentrations.F - The results of study showed that the addition of 20,40,60% sea water led to a significant increase in the rate 0f effectiveness 0f the enzyme Super oxide dismutase (S.O.D) and enzyme Ascorbate peroxides (APX),reaching the highest rate of effectiveness of the enzyme at the concentration 0f 60% compared with the comparative treatment , whil the highest average of the effectiveness of enzyme Catalase (CAT)and glutathione piroxidase (GP)and polyphenoloxidase (ppo)was at the concentration 20%sea water then it started to decline when increasing the concentration of sea water from 40 to 60% As for the algae extract it has been shown from the results of the current study that the addition of algae extract to nutrient medium to a high effectiveness of enzyme SOD,CAT ,APX and GP and more significant with all concentration of sea water The impact of algae extract on the effectiveness of the enzyme PPO has led to increasing the effectiveness of this enzyme but did not amount to a significant level except with sea water nutrient medium .G - The results of the current study showed a significant increase of the Amber cutivar in the percentage of the callus induction and the required time of this induction as well as the Wight of the initial and embryo callus ,number and germination percentage of thesomatic embryo , number of plantlets ,as wall as the percentage of the accumulation of potassium ,phosphorus ,calcium, and manganese ions ,as will as in the potassium/sodim ratio focus histidine proline and the effectiveness of the enzyme SOD and CAT enzyme and enzyme GP. While the product was higher than in the accumulation of Firat magnesium ion ,zinc copper and in the effectiveness of the enzyme in APX & PPO . .

دراسة وبائية وجزيئية للجرثومة Escherichia coli O157 : H7 المعزولة من عينات سريرية وبيئية في محافظة البصرة - العراق == Epidemiological and molecular study of the bacterium Escherichia coli O157 : H7 isolated from clinical and environmental samples Basra Governorate / Iraq

Author name: عباس ضرب شعبان
Supervisor name: ميثم ايوب عبد القادر الحمداني
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: A total of 464 clinical and environmental samples attempt to isolate Escherechia coli O157 : H7 serotype were collected, of which 183 stool samples from diarrheal children at Al - Qurna hospital and Al - Medaina Hospital of children during the period between October 2013 - August 2014. Environmental samples (281) : 121 samples of drinking ( tap) water , 80 samples of river water , and 80 samples of sewage water (collected from different regions of Basra Governorate) . All samples was tested and detected the presence of Escherichia coli serotype O157 : H7 using chromogen agar and ( CT - SMA ) as selective media and using direct immunological testing for direct identification of the pathogen (Certeste) for clinical samples.The study revealed the isolation of 56 isolates of E.coli O157 : H7 at a rate of (12.06%) : 16 clinical isolates at a rate of (8.7%), 40 environmental isolates at a rate of (14.23) : 9 isolates from drinking water at a rate of (7.4%), 18 isolation from sewage at a rate of (22.5), and 13 river isolates at a rate of (16.25).Antibiotic susceptibility testing toward 20 antibiotic of E.coli O157 : H7 isolates showed that all isolates from all sources resistant to the antibiotics Ampicillin, Amoxacilllin, , Rifampin and Piperacillin while showed high sensitivity to antibiotics amikacin, Kanamcin, ciprofloxacin and norfloxacin.The clinical isolates of E.coli O157 : H7 showed multiple high antibiotic resistance index Muliple Antibiotics Resistance (MAR index) ranged between (0.25 - 0.8), Where two isolates showed a resistance to sixteen antibiotics, Two isolates showed resistance to twelve antibiotics, Two isolates showed a resistance to eleven antibiotics, one isolate showed a resistance to ten antibiotics,Three isolates showed a resistant to nine antibiotics and two isolates showed aresistance to eight antibiotics, One isolate showed a resistant to six antibiotics and two isolates showed resistance to five antibiotics.The environmental isolates of E.coli O157 : H7 showed less resistance ranged between (0.25 - 0.75) for samples of sewage and (0.35 - 0.50)rivers samples , and (0.30 - 0.55)tap water samples . As shown, one isolate resistant to fifteen antibiotics, one isolate showed a resistance to thirteen antibiotics and four isolates showed a resistance to ten antibiotics from sewage. As for rivers Tow isolates showed a resistance to ten antibiotics, three isolates showed a resistant to nine antibiotics while a one isolate of tap water showed a resistance to eleven antibiotics, three isolates showed a resistance to ten antibiotics and an one isolates showed a resistance to nine antibiotics and two isolates resistant to eight antibiotics.Investigating about the content of plasmid in isolates E.coli O157 : H7 to study the relationship between the plasmid content as an indicator of antibiotic resistance, as the results showed that most of the isolates E.coli O157 : H7 four clinical isolates containing the plasmid including one molecular weight > 10kbp with a Patterns of multiple different resistance,also two isolates contain three plasmids of a molecular weight > 10kb. And one isolate containing four plasmids with molecular weights ranging from (4 - 10Kb) and one isolate containing five plasmids resistant to sixteen antibiotics with molecule weights ranged between >(2 - 10Kb).The isolates E.coli O157 : H7 isolated from the environment , sixteen isolates showed that they contain plasmids of molecular weights> 10kb , one isolate contains two plasmids of molecular weights ranging between (4 - 10Kb), five isolates containing three plasmids of molecular weights ranging from (2.9 - 10) Kb and two isolates containing four plasmids of molecular weights ranging between( 2 - 10 Kb

دراسة بعض التاثيرات الفسيولوجية والنسجية لدواء Paclitaxel المضاد للسرطان في الفئران المختبرية Mus musculus L. == Study of some physiological and histoloygical effects of the anticancer drug paclitaxel in laboratory mice Mus musculus L

Author name: ضحى طالب حميد الطائي
Supervisor name: سامي جبر المالكي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: صممت الدراسة الحالية لمعرفة تاثير دواء Paclitaxel المضاد للسرطان في الجوانب الفسيولوجية والكيموحيوية والنسجية في الفئران المختبرية البيض Mus musculus L. , اذ تم تقسيم ذكور الفئران المختبرية واناثها الى ثلاث مجاميع , تتكون كل مجموعة من ثمانية فئران هي : مجموعة السيطرة Control group : تتكون من 8)) ذكور و(8) اناث , حقنت بـ (0.2) مل من المحلول الفسيلوجي Normal Saline داخل الغشاء البريتوني (I.P) .مجموعة الجرعة الواطئة : تتكون من (8) ذكور و(8) اناث, حقنت بـ (0.2) مل من محلول دواء Paclitaxel بتركيز 12 ملغم / كغم داخل الغشاء البريتوني (I.P) .مجموعة الجرعة العالية : تتكون من (8) ذكور و(8) اناث , حقنت بـ (0.2 ) مل من محلول دواء Paclitaxel بتركيز 24 ملغم /كغم داخل الغشاء البريتوني (I.P) . وقد اوضحت نتائج الدراسة الحالية حدوث انخفاض معنوي في معايير الدم المتضمنة تعداد كريات الدم الحمر, وحجم الدم المضغوط وخضاب الدم في كل من ذكور الفئران المختبرية واناثها المعاملة بالجرعتين ( 12 و24) ملغم /كغم وانخفاض معنوي في معدل حجم الكرية الحمراء بالجرعة العالية في الاناث عند مقارنتها بمجموعة السيطرة . كما بينت النتائج انخفاضا معنويا في التعداد الكلي لخلايا الدم البيض والنسبة المئوية للخلايا الوحيدة والحبيبية في ذكور الفئران المختبرية المعاملة بعقار Paclitaxel بالجرعتين (12 و24) ملغم \كغم بينما لوحظ في الاناث انخفاض معنوي في العدد الكلي لخلايا الدم البيض والنسبة المئوية للخلايا الوحيدة بالجرعتين والحبيبية بالجرعة العالية 24 ملغم \كغم عند مقارنتها مع مجموعة السيطرة, وارتفاع معنوي في النسبة المئوية للخلايا اللمفاوية بالجرعتين 12)و 24) ملغم \كغم لذكور الفئران المختبرية واناثها. اظهرت النتائج وجود انخفاض معنوي في وزن جسم ذكور الفئران المختبرية واناثها بالجرعة العالية 24 ملغم / كغم فقط عند مقارنتها مع مجموعة السيطرة. كما اوضحت النتائج حدوث انخفاض معنوي في نسبة خصوبة ذكور الفئران المختبرية واناثها (عدد الحوامل) المعاملة بعقار Paclitaxel بالجرعتين ( 12و24 ) ملغم / كغم , وحدوث انخفاض معنوي في عدد مواليد الامهات والذكور المعاملة بالجرعتين , كما اظهرت النتائج انخفاضا معنويا في اوزان المواليد عند اليوم الاول للولادة والاسابيع الخمسة بعد الولادة في ذكور الفئران المختبرية المعاملة بالجرعتين بينما لوحظ في الاناث المعاملة بالجرعتين (12 و24 ) ملغم \كغم انخفاض معنوي في اوزان الولادات عند اليوم الاول للولادة والاسابيع الثلاثة بعد الولادة وبالاسبوع الخامس بالجرعة العالية عند مقارنتها بمجموعة السيطرة . سبب دواء paclitaxel انخفاضا معنويا في اعداد الحيامن بالجرعتين 12)و (24ملغم /كغم وخلال المدتين 45, 30 يوما , بينما لم تظهر النتائج فرقا معنويا في تشوهات ذيل النطف ورؤوسها بالجرعتين وخلال المدتين عند مقارنتها مع مجموعة السيطرة , فضلا عن ذلك بينت النتائج حدوث انخفاض معنوي في اعداد الحيامن السوية بالجرعتين وخلال المدتين. اظهرت نتائج الدراسة الهرمونية انخفاض معنوي في مستوى هرمون الشحمون الخصوي والهرمون المحفز للجريبات stimulating hormone (FSH) Follicle والهرمون اللوتيني Luteinizing hormone (LH) بالجرعتين في ذكور الفئران المختبرية, بينما لوحظ في الاناث انخفاض معنوي في هرمون الاستروجين بالجرعة العالية 24 ملغم /كغم , وانخفاض في الهرمون المحفز للجريبات بالجرعتين عند مقارنتها مع مجموعة السيطرة . كما بينت نتائج الدراسة الحالية وجود ارتفاعا معنويا في مستوى انزيمات الكبد المتمثلة بانزيم Aspartate transaminase ( AST) و(Alanine transaminase ( ALT وAlkaline phosphatase (ALP) بالجرعة العالية 24 ملغم / كغم في ذكور الفئران المختبرية عند مقارنتها مع مجموعة السيطرة . كما اظهرت نتائج الدراسة ايضا انخفاض معنوي في مستوى البروتين الكلي في ذكور الفئران المختبرية واناثها عند معاملتها بالجرعة العالية 24 ملغم / كغم فقط مقارنة مع مجموعة السيطرة. اشارت نتائج الدراسة الحالية الى ارتفاع معنوي في مستوى الدهون المتعادلة ثلاثية الكليسريد بالجرعة العالية في كلا الجنسين وانخفاضا معنويا في مستوى الدهون المتعادلة ثلاثية الكليسريد في ذكور بالجرعة الواطئة 12 ملغم/كغم وارتفاعا معنويا في مستوى الكولسترول الكلي لمصل الدم في الذكورفقط بالجرعة العالية 24 ملغم /ملغم وارتفاع معنوي في مستوى البروتين الدهني عالي الكثافة High density lipoprotein في ذكور الفئران المعاملة بالجرعة العالية 24 ملغم \كغم وبالجرعة الواطئة 12 ملغم \ كغم في الاناث , كما سبب الدواء انخفاضا معنويا في مستوى البروتين الكلي واطئ الكثافة جدا Very low density lipoproteinفي الذكور بالجرعة الواطئة عند مقارنتها مع مجموعة السيطرة . واظهر حقن دواء Paclitaxel في الفئران المختبرية حدوث ارتفاع معنوي في مستوى اليوريا لمصل دم ذكور بالجرعتين ( 12 و24 ) ملغم\كغم , بينما في الاناث بالجرعة العالية عند مقارنتها مع مجموعة السيطرة . واظهرت نتائج دراسة المقاطع النسجية في خصى الفئران المختبرية المعاملة بدواء Paclitaxel حدوث تحلل كامل في جدار معظم النبيبات المنوية وفرط تنسج في خلايا ليدك بالاضافة الى نزف في النسيج الضام البيني , وخلل تنسج Dysplasia . وبينت المقاطع النسجية في مبيض الفئران المختبرية المعاملة بالدواء حدوث احتقان بعض الاوعية الدموية , وتحلل خلية البيضة , وتنخر خلايا الطبقة الحبيبية وانفصالها عن خلية البيضة وعدم تكون طبقة خلايا الاكليل المشع Corona radiate بالاضافة الى تحلل نواة الحوصلة الثانوية . كما سبب دواء Paclitaxel تغيرات مرضية نسجية في كبد وكلى الفئران المختبرية , اذ اظهرت نتائج المقاطع النسجية لكبد ذكور الفئران واناثها المعاملة بالدواء حدوث تنخر وتنكس في الخلايا الكبدية ,وارتشاح الخلايا الالتهابية مع ظهور تشوه وتضخم انوية بعض الخلايا الكبدية وفرط تصبغ السايتوبلازم ,بالاضافة الى توسع الوريد المركزي والاوعية الدموية ,وتوسع الوريد البابي الكبدي واشباه الجيوب الكبدية واحتقانها . اظهرت الدراسة النسجية لكلى ذكور الفئران المختبرية واناثها المعاملة بالدواء حدوث تنخر بطانة النبيبات وتنكسها , وارتشاح في الخلايا الالتهابية مع ظهور تشوهات في الانوية وحدوث موت خلوي مبرمج , واحتقان في الاوعية الشعرية الدموية, وتوسع تجويف النبيب الملتوي القريب واختفاء حافته الفرشاية, وتوسع الاوعية الشعرية الدموية, ونزف وتضخم وفرط تنسج وتوسع, وتضيق حيز محفظة بومان في الكبيبة , وحؤول بطانة النبيبات, وانتفاخ بعض خلايا النبيبات الكلوية وتضخمها . | This study was designed to investigate the effects of the anticancer drug Paclitaxel on some physiological, Biochemical parameters and histopathological changes in albino laboratory mice Mus musculus L. , The male and female laboratory mice were divided into three groups, eight mice each : 1 - The control group (8) males and (8) females , all of them injected with (0.2)ml of physiological saline Intraperitoneal (I.P) .2 - The low dose group of (8) males and (8) females, all of them injected with (0.2) ml of 12 mg/kg paclitaxel , Intraperitoneal (I.P) . 3 - The high dose group of (8) males and (8) females, all of them injected with (0.2) ml of 24 mg/kg paclitaxel , Intraperitoneal (I.P) . The results of this study showed a significant decrease (P≤0.05) in blood parameters that include red blood corpuscle (RBC) , packed cell volume (PCV) and haemoglobin (Hb) for both male and female mice were injected with two doses of paclitaxel (12,24) mg/kg and a significant decrease in mean cell Volume (MCV) that are treated with high dose. The results also showed a significant decrease in the total number of white blood cells and percentage of monocyte and granulocyte cells in male mice treated paclitaxel for both two doses (12,24) mg/kg . while in female mice the total number of white blood cells and percentage of monocyte cells were significant decreased with two doses and percentage of granulocyte cells with high doses 24 mg\kg in comparison with the control group, and a significant increase in percentage of lymphocyte in male and female mice were treated with the two doses(12,24) mg/kg. The results also exhibited a significant decreased in body weight of male and female mice in high dose 24 mg/kg only when compared with control group. The results exhibited a significant decrease in fertility of male and female mice treated with paclitaxel with two doses (12 ,24) mg\kg, there was a significant decrease also in number of newborns male and female treated with two doses, and there was a significant decrease in newborns weight in the first day of birth and the five weeks of birth with (12,24) mg/kg , while female treated with the two doses , there was a significant decrease in the first day of birth and subsequent three weeks after birth with the two dose and fifth week with the high dose ( 12 , 24) mg/kg in comparison with the control group . The results of this study also showed a significant decrease in sperm count treated in mice with the two doses after 30 - 45 days of birth . while no significant difference in malformation rate of sperm (head and tail) with the two doses through the same period in comparison with the control group . there was a significant decrease in the number of normal sperm through the same period with the two doses . The hormonal study showed a significant decrease in testosterone level and follicle stimulating hormone (FSH) with both doses and luteinizing hormone (LH) level with two doses for male mice , in females there was a significant decrease in estrogen levels with high dose 24 mg\kg and a significant decrease in follicle stimulating hormone (FSH) with two doses in comparison with control group. The present study showed a significant decrease in levels of liver enzymes Aspartate transaminase (AST) , Alanine transaminase (ALT) and Alkaline phosphatase (ALP) in male mice were treated with high dose (24) mg/kg in comparison with control group . The present study also showed significant decreased in total protein in male and female mice treated with high dose (24) mg/kg only in compared with control group. The study showed a significant increase in triglycerides level in male and female mice that are treated with high dose and it also showed a significant decrease in triglycerides level in male mice with low dose 12 mg/kg .There is a significant increase in the total cholesterol level for serum in male mice only that are treated with high dose 24 mg/kg .The study showed a significant increase in high density lipoprotein (HDL) in male mice with high dose 24 mg/kg and even with the low dose 12 mg\kg in female mice . The drug causes a significant decrease in very low density lipoprotein (VLDL) in male mice that are treated with the low dose in comparison with the control group . Injection with paclitaxel in mice lead to a significant increase in urea level in serum for male mice that are treated with both doses (12,24) mg\kg in comparison with the control group. The results of the study of histological sections in male testis were treated with paclitaxel showed analysis in most seminiferous tubules , hyperplasia in leydig cell , bleeding in interstitial connective tissue and dysplasia. Histological sections in female mice ovary were treated with paclitaxel showed a congestion in some blood vessel , oocyte degeneration, necrosis of granular layer and separated from oocyte and degeneration of secondary follicle nuclei. Injection with paclitaxel leads to histopathological pathological changes in mice kidney and liver .The result of histopathological sections of liver in male and female mice were injected with paclitaxel showed necrosis and degeneration in hepatocytes, infiltration of inflammatory cells with show malformation and hypertrophy of some hepatocytes nuclei , hyperchromic cytoplasm, also dilation in central vein and blood vessel , dilation and congestion of portal hepatic vein and sinusoids. The study of histological sections for male and female kidneys were treated with paclitaxel showed necrosis of endothelial tubules and infiltration of inflammatory cells with show malformation in nuclei and apoptosis , congestion in blood vessel , dilation in proximal distal tubules and absent of brush border , dilation capillaries , bleeding, hypertrophy , hyperplasia, dilation and narrow of bowman's capsule , metaplasia endothelial tubules , swelling cells and hypertrophy of some renal tubules cells .

تقدير الهستامين في بعض الاغذية المحلية وتحديد جينات الهستدين (hdc) في البكتريا المعزولة منها ودورها في تراكم الهستامين == Estimation of histamine in some local food and detection of histidine genes (hdc) from their isolated bacteria and it’s role in accumulation of histamine

Author name: صلاح ناجي عزيز الخيون
Supervisor name: قيثار رشيد مجيد | طالب احمد جايد
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: ELISA technique was used to estimate of histamine in muscles of several types of local fresh fish, including : Tenualosa ilisha, Megalaspis cordyla , Chirocentrus dorab and Scomberoides commersonianus in addition of the shrimp Metapenaeus affinis , all samples of fish and shrimp were collects from Fao markets in Basarah city .Results showed the percentages of histamine in all types of fish, as well as shrimp were : 4.483 and 3.953 and 3.353 and 3.16 and 2.03 mg/kg respectively.Accumulation of histamine in fish after refrigerating for three days was also determinated. Results showed that the values of histamine accumulate were increased within preservation period .On the third day of histamine accumulate of C. dorab , T. ilisha , M. cordyla , S. commersonianus and shrimp were 4.56 , 3.99 , 3.43 , 3.19 and 2.01 mg / kg, respectively. While , on the first day were 4.01 , 3.65 , 3.03 , 2.98 and 1.89 mg/kg respectively .ELISA technique was also used to estimate histamine in Ripening cheese such as cheddar , Alkuda , Almoszerla and Oloadam cheese and smoked Hajdu . They were 4.267 , 2.433, 1.167 , 3.667 and 1.833 mg / 100 g , respectively.Many bacteria were isolated isolated from fishes and shrimp, they identified according to : growth characteristics on selective media, morphological, and microscopic examinations in addition to biochemical tests.Fifty three isolates of bacteria were found, they were : Klebsiella pneumonia sub. pneumonia , Klebsiella oxytoca , Morganella morganii sub. Morganii, Enterobacter aerogenes, Enterobacter cloacae , Cronobacter sakazakii ,AEnterobacter taylorae, Citrobacter freundii , Citrobacter amalonaticus , Proteus marina, Proteus vulgaris , Proteus mirabilis, Hafnia alvei , Escherichia coli, Serratia marcescens, Serratia fonticola , Vibrio vulnificus, Vibrio diazotrophicus , Vibrio hollisae , Vibrio alginolyticus, ,Vibrio cholera, Vibrio parahaernolyticus, Vibrio fluvialis, Lactobacillus helveticus, Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus johnsonii, Lactobacillus salivarius, Pseudomonas aeruginosa, Pseudomonas fluorescens, Staphylococcus saprophyticus, Staphylococcus aureus , Staphylococcus intermedius , Staphylococcus epidermidis, Bacillus pumilus , Bacillus licheniformis , Bacillus firmus , Bacillus subtilis, Bacillus alvei , Aeromonas. caviae , Aeromonas veronii bv.veronii , Aeromonas eucrenophila, A. sobria , Aeromonas veronii and Aeromonas encheleia .Isolation and identification of Lactobacillus from all samples of ripening cheese were done. Six species of Lactobacillus were found and as a follow : Lactobacillus plantarum , Lactobacillus delbrueckii, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus fermentum and Lactobacillus paracasei .DNA was extracted from all types of G+ and G - bacteria which isolated from fish and shrimp in addition to Lactobacillus bacteria that isolated from ripening cheeses . the DNA of all bacteria was good and pure .Polymerase chain reaction (PCR) technique was used for identification all isolates of Lactobacillus which were isolated from fish and shrimp, as well as ripening cheese depending on the bacterial 16S rRNA gene sequences of bacteria with using different types of diagnosed primers, genes had been identified for : Lactobacillus acidophillus , Lactobacillus plantarum , Lactobacillus delbrueckii , Lactobacillus fermentium , Lactobcillus helveticus , Lactobcillus crispatus ,BLactobcillus gasseri, Lactobcillus reuteri , Lactobcillus rhamnosus, Lactobcillus paracasei, Lactobacillus johnsonii , Lactobacillus salivarius and Lactobacillus casei .Nine primers were in RAPD technique to find out the genetic link between different strains of Lactobacillus bacteria, the results showed that the number and location of randomized bands were differed in some bacterial species but others was fitted and produced a variety genetic of bacterial strains, in particular a number of bands as possible to get a variety of different genetic profiles among Lactobacillus isolates .The results appeared different styles of bands and presence of specific bands in Lactobacillus bacteria in all primers . The results obtained from RAPD technique showed that the total number of bands were 630 bands, the total number of bands which formed genetically was 127 and the percentage of total proportion of genetic variation among the isolates was 20.15% .The results found of genetic primers scheme that the genetic distance was as close as possible between bacteria L.fermentum and L.rhamnosus and was a part of the one genetic group, but L.helveticus was close as possible genetically to this group , and then the L.johnsonii and L.acidophilus was more genetically distant to this group. Also it found that the genetic distance was very near between L.plantarum and L.paracasei and were a part of a one hereditary group and found also that L.reuteri closest genetically to this group, then followed by more genetically L.delbrueckii . However, L.acidophilus was also more genetically distant to this group .CThe results revealed that L.salivarius and L.crispatus were a one hereditary group and the genetic distance for this group was very close to the L.gasseri and it was farther genetically for genetic group, which includes L.fermentum and L.rhamnosus , also found that the bacteria L.casei showed far genetic distance between them and the groups of Lactobacillus .All fifty three isolates of bacteria which isolated from fish , shrimp and ripening cheeses that producing and did not producing histamine subjected to amplified genes which responsible of the histamine production by using the primers HIS1 - F/HIS1 - R ; JV16HC/ JV17HC ; Hdc - f/ Hdc - r ; 106/107 and UNI - L/ UNI - R . Amplification results showed that all primers used to amplified genes of histamine gene different genes sizes depending on the types of bacteria when were positive or negative to Gram stain and their OrigenIt was found the size of the gene was 350 base pairs , was found in G+ bacteria which isolated from fish , shrimp and cheese ripening . These bacteria included : L.acidophilus ,L.helveticus ,L.crispatus, L.gasseri ,L.casei , L.reuteri , L.fermentum,L.rhamnosus, L.paracasei, L.johnsonii, L.salivarius L.plantarum , L.delbrueckii , S.aureus , S. saprophyticus , S.epidermidis , S. intermedius , Bacillus pumilus, B.subtilis, B.licheniformis, B.firmus and B. alvei .May same results were found when amplified HISI - R/HISI - F primer , the size of histamine gene most positive Gram bacteria stain .High size of histamine gene 709 base pair appeared when amplified Hdc - r/ Hdc - f primer .This gene was found in G - bacteria which isolated from fish and shrimp . These bacteria included : Pseudomonas aeruginosa , Pseudomonas fluorescens, Klebsiella pneumonia , Morganella morganii , Enterobacter aerogenes, KlebsiellaDoxytoca, Hafnia alvei, Enterobacter cloacae, Vibrio alginolyticus, Cronobacter sakazakii, Escherichia coli , Enterobacter taylorae, Proteus vulgaris, Proteus mirabilis, Vibrio vulnificus , Vibrio diazotrophicus, Vibrio hollisae , Vibrio fluvialis , Serratia marcescens and Serratia fonticola .However , the size of histamine gene was 530 base pairs , when amplified 106 / 107 primer , the gene was found in most G - bacteria .On the other hand , the UNI - L/UNI - R primer did not amplify in all isolates of bacteria which isolated from fish , shrimp and ripening cheeses .Extraction of plasmids from all isolated bacteria was done . Results showed that isolates have different types of plasmids bands (small and large size) and when the electric deportation of the genetic material of plasmids on gel agarose while some other isolates appeared possess one plasmid.In this study , PCR technique was not detected the presence of histamine genes in plasmid of bacteria , because not occurred any amplify for any type of primers used . This indicates the presence of histamine genes on the genome of the bacteria and not on plasmids of bacteria .

دراسة بعض التاثيرات الفسيولوجية والنسجية لمبيد دايمثويت في الفئران المختبرية البيض Mus musculus L. == Study of some Physiological and Histological Effects of Dimethoate pesticide in laboratory white mice Mus musculus L

Author name: صفا محمد حسين
Supervisor name: علي عبد اللطيف العلي | فارس شاكر كاطع
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: تم دراسة التاثيرات الفسيولوجية والنسجية في بعض اعضاء الفئران المختبرية بعد حقنها بجرعتين من المبيد الحشري الفوسفوعضوي دايمثويت Dimethoate, اذ قسمت ذكور الفئران المختبرية الى ثلاث مجاميع وضمت كل مجموعة ثمان فئران وبالطريقة نفسها قسمت اناث الفئران وحقنت مجاميع الذكور والاناث كالاتي : - 1مجموعة السيطرة : حقنت بحجم 0.1 مل من المحلول الفسيولوجي .Normal saline 2 - مجموعة المعاملة الاولى Treated group 1 (T1) : حقنت بحجم 0.1 مل من محلول مبيد dimethoate وبتركيز 6ملغم/ كغم ولمدة 30 يوما ومثلت الجرعة الواطئة من المبيد. - 3 مجموعة المعاملة الثانية Treated group 2 (T2) : حقنت بحجم 0.1 مل من محلول مبيد dimethoate وبتركيز12 ملغم/ كغم ولمدة 30 يوما ومثلت الجرعة العالية من المبيد. بينت نتائج المعايير الدمية لذكور الفئران المختبرية وجود انخفاض معنوي في كل من عدد كريات الدم الحمر وخضاب الدم وحجم الدم المضغوط وبالجرعتين 6 و12 ملغم/ كغم مقارنة بمجموعة السيطرة, كما اظهرت النتائج انخفاض معنوي في معدل تركيز خضاب دم الكرية MCHC وبالجرعة 12 ملغم / كغم فقط ، اما في الاناث فقد انخفض معنويا عدد كريات الدم الحمر وحجم الدم المضغوط ومعدل حجم الكرية ومعدل تركيز خضاب دم الكرية وبالجرعتين6 و12 ملغم/ كغم عند المقارنة بمجموعة السيطرة, في حين انخفض معنويا معدل خضاب الدم Hb بالجرعة الواطئة 6 ملغم/ كغم فقط عند مقارنتها بمجموعة السيطرة. اظهرت نتائج العد التفريقي لخلايا الدم البيض في ذكور الفئران انخفاضا معنويا في عدد خلايا الدم البيض الكلي وبالجرعتين6 و12 ملغم/ كغم، كما انخفضت معنويا كل من النسبة المئوية للخلايا الحبيبية والوحيدة بالجرعة العالية 12 ملغم/ كغم فقط عند مقارنتها بمجموعة السيطرة ,في حين انخفض عدد خلايا الدم البيض الكلي والنسبة المئوية للخلايا الحبيبية في اناث الفئران المحقونة بالجرعة 12 ملغم/ كغم فقط من المبيد عند المقارنة بمجموعة السيطرة. اوضحت نتائج الدراسة الحالية وجود انخفاض معنوي في اعداد الحيامن بالجرعتين6 و12 ملغم/ كغم, ووجود ارتفاع معنوي في نسبة تشوهات رؤوس الحيامن وذيولها فضلا عن وجود انخفاض معنوي في اعداد الحيامن الطبيعية وبالجرعتين 6 و12 ملغم/ كغم مقارنتها بمجموعة السيطرة. بينت نتائج الدراسة الحالية وجود انخفاض معنوي في الزيادة الوزنية لذكورالفئران المختبرية واناثها المعاملة بالجرعتين 6 و12 ملغم/ كغم من مبيد الدايمثويت مقارنة بمجموعة السيطرة. اوضحت النتائج عدم وجود فروق معنوية في مستوى انزيمات كبد ذكور الفئران المختبرية Aspartate transaminase (AST) وAlkaline phosphatase (ALP) المعاملة بالجرعتين6 و12 ملغم/ كغم, فيما انخفض معنويا مستوى انزيم Alanine transaminase (ALT) بالجرعة الواطئة 6 ملغم/ كغم فقط مقارنة بمجموعة السيطرة, في حين ارتفع معنويا مستوى انزيمات AST وALP لكبد اناث الفئران بالجرعتين6 و12 ملغم/ كغم فضلا عن ارتفاع مستوى انزيم ALT وبالجرعة الواطئة 6 ملغم/ كغم فقط مقارنة بمجموعة السيطرة. كما اشارت النتائج الى ارتفاع معنوي في مستوى يوريا وكرياتنين المصل لذكور الفئران وبالجرعة 12 ملغم/ كغم فقط, كما ارتفع معنويا مستوى اليوريا فقط لمصل اناث التجربة بالجرعة العالية 12 ملغم/ كغم فقط ولم تظهر النتائج اي فارق معنوي في تركيز كل من اليوريا والكرياتنين لمصل مجموعة الجرعة الواطئة 6 ملغم/ كغم مقارنة بمجموعة السيطرة. اظهرت نتائج فحص المقاطع النسجية لاعضاء الفئران المختبرية حدوث تغيرات نسجية مرضية في انسجة كبد وكلى ورئة وطحال وخصى ومبايض الفئران المحقونة بمبيد دايمثويت وبالجرعتين 6 و12 ملغم/ كغم واعتمدت شدة التغيرات على جرعة المبيد اذ ازدادت شدتها في الجرعة العالية 12 ملغم/ كغم, وتمثلت التغيرات في الكبد بحدوث تنكس Degeneration وتنخرNecrosis لبعض خلايا الكبد واحتقان دموي Congestion وتوسع Explansions في الوريد البابي والمركزي واشباه الجيوب الكبدية فضلا عن وجود نزف Hemorrhage في المساحة البابية وارتشاحInfiltration خلايا التهابية في مناطق متفرقة في الكبد, اظهرت التغيرات في الكلية تنكس بعض خلايا بطانة النبيبات في حين لوحظت خلايا اخرى متنخرة, اضافة الى انسلاخSloughing وحؤولMetaplasia بعض خلايا بطانة النبيبات بالاضافة الى احتقان ونزف وارتشاح خلايا التهابية في الكبيبات . اظهر الفحص النسجي لرئة الفئران المحقونة بجرعتي المبيد تغيرات نسجية مرضية متمثلة بوجود وذمات Odema وارتشاح خلايا التهابية بمناطق مختلفة ونزف واحتقان الاوعية الدموية, فضلا عن حدوث فرط تنسج Hyperplasia وانتفاخ Swelling الخلايا المبطنة للاسناخ الرئوية مما يسبب تضييق تجاويف تلك الاسناخ. اما في الطحال فقد تمثلت التغيرات النسجية بحدوث تضخم للعقيدات الطحالية وتوسع الاوعية الدموية وقلة في اعداد خلايا متعددة الانوية الكبيرة , فضلا عن حدوث زيادة في خلايا النسيج النخاعاني myloid tissue المكون للدم ووجود تكدس مواد غير حية شفافة materials Hayline بلون وردي في مناطق اللب الاحمر لاسيما حول العقيدات الطحالية . ادى الحقن بمبيد الدايمثويت الى حدوث تغيرات نسجية مرضية في انسجة خصى ومبايض الفئران المختبرية اذ تمثلت هذه التغيرات بحدوث تحلل كامل لمعظم الخلايا المكونة للحيامن في النبيبات المنوية مما يؤدي الى قلة اعدادها, كما لوحظ تسمك Thicking الغشاء القاعدي لبعض النبيبات المنوية فضلا عن احتقان الاوعية الدموية ووجود وذمات بين النبيبات, اما التغيرات النسجية التي حدثت في المبيض شملت حدوث فرط تنسج واحتقان الاوعية الدموية في برنكيما المبيض. | The physiological and histopathological changes were studied in some organs of laboratory mice injected with two doses of organophosphorus insecticide (Dimethoate). male mice were divided into three groups of eight animals per groups, and the same for females and male and female groups were injected as following : 1 - Control group : were intraperitonially (i.p) injected with 0.1 ml of normal saline.2 - Treated group 1 (T1) : were i.p injected with 0.1 ml of 6mg/kg of Dimethoate for a period of 30 days and represented as a low dose of the pesticide.3 - Treated group 2 (T2) : were i.p injected with 0.1 ml of 12 mg/kg of Dimethoate for a period of 30 days and represented as a high dose of the pesticide. The results of blood parameters in male mice showed a significant decrease in both of red blood corpuscle (RBC) count, hemoglobin Hb and Packed cell volume (PCV) of both doses (6,12mg/kg), and significant decrease in mean cell hemoglobin concentration (MCHC) in dose of 6mg/kg only as compared with control group. while in female, both of RBC count,PCV, mean cell hemoglobin(MCH) and MCHC were significantly decreased in both doses (6,12mg/kg), a significant decreased in Hb in dose of 6mg/kg in comparision with control group were recorded. The results of differential WBC counts of male mice showed significant decrease in total WBC counts in mice injected by both doses (6,12mg/kg),also the percentage of monocytes and granulocytes were significantly decreased in dose of 12mg/kg only as compared with control group, while the WBC count and the percentage of granulocytes were significantly decresed in female injected with 12mg/kg only of pesticide as compared with control group. In the male mice injected with two doses (6,12mg/kg). the results of current study explained decrease significantely in sperm count, and significant increases in percentage of malformation rate of sperm heads and tails , as well as, significant decreases in normal sperm count when compared with control group. The results of present study showed significant decrease in weight gain for male and female laboratory mice treated with two doses (6,12mg/kg) of dimethoate in comparision with the control group. The present data showed no significant differences in the level of liver enzymes Aspartate transaminase (AST) and Alkaline phosphatase (ALP) of male laboratory mice treated with two doses (6,12mg/kg), while significant decreased in the level of Alanine transaminase (ALT) enzyme with dose of 6 mg/kg only as compared with control was noticed, while significantely increased in levels of liver enzymes (AST and ALP) in female mice with two doses (6,12mg/kg), as well as,there was a significant increased in level of ALT enzyme with dose 6 mg/kg only when comparision with control. The level of urea and creatinine serum significantly increased whith two doses (6,12mg/kg), and there was no significant difference with dose of 6mg/kg when compared with control, also, the level of urea significantly increased in the serum of female mice with 12 mg/kg only, and there was no significant differences with 6 mg/kg as compared with control group. The results of histological section for some organs of laboratory mice showed occurrence of histopathological changes in the tissues of liver,kidney,lungs,spleen,testis and ovaries of mice injected with doses (6,12mg/kg), and The severity of the effects were dependent on dose, Histopathological findings of liver included degeneration and necrosis of some liver cells, blood congestion and explansions in the portal vein,central vein and hepatic sunsoids, as well as, the presence of hemorrhage in the portal area, infiltration of inflammatory cells in scattered area of it. In the kidney, histopathological changes showed degeneration of some tubular epithelium,while other cells where observed necrotic, sloughing and metaplasia of some tubular lining cells, as well as, congestion, hemorrhage and infiltration of inflammatory cells in the glumeruli. The histological examination of mice lungs were injected with two doses of dimethoate showed histopathological changes represented by the presence of edemas, infiltrations of inflammatory cells in different areas, hemorrhages and vascular congestion, as well as the occurrence of hyperplasia and swelling of cells lining of the pulmonary alveoli, causing narrowing cavities of those alveoli. In the spleen, the histological changes represented by occurrence of hypertrophy of the spleen nodules, expansion of blood vessels and decrease in the number of megalomultinucleated cells, as well as an increase in the cells of hematopoietic myloid tissue and presence of hyaline material, stained with pink color in the red pulp areas, especially around the spleen nodules. Injection with dimetoate caused histopathological changes in the testis and ovaries of laboratory mice represented by complete analysis of most spermatogenic cells in the seminiferous tubules,leading to decrease their numbers ,we also observed thickening of the basement membrane of some seminal tubule ,as well as vascular congestion and deposition of a live materials in interstitial spaces ,while the histological changes that have occurred in the ovaries included hyperplasia and vascular congestion in the stroma in addition to decrease number of ovarian follicles and corpora leutea.

تقييم تلوث الهواء باستعمال دليل نوعية الهواء AQI ودليل التحول APTI لبعض المواقع المختارة في محافظة البصرة == Assessment of air pollution using AQI and APTI indices in selected locations of Basra governorate

Author name: شيماء هشام شهاب الدين
Supervisor name: نايف محسن عزيز
General topic: Biology
Specific topic: Environment - Environmental pollution
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study has been done for period from December to January 2015 including five station in three replicate for each station distributed in Basra Province (Al - Qurna district ,city center - Ashar ,Altenuma, and Zubair as well as region which is called Abu Al Khaseb , it is chosen as a control station for being agricultural area. The samples have been collected monthly for evaluating the parentage of gas pollution concentration (CO2,CO,NOx,SO2 and TSP) . The concentration of gas is measured by using gas analyzer device and the mount of particles in the air is measured by PDR - 1200. And black carbon ( BC) was measured by using Aethalometer . AQI was using for air quality for knowing the quality of surrounding air . Maps are drawn which show the spreading pollutants in Basra Province during the season by using GIS program . it is clear that there was differences concentration rates of pollutant gases in Basra province, the seasonally difference for gases pollutant proved that in Winter recorded high value for gases CO2, CO which reached 258.62 ppm for CO2 and 7.19 ppm for CO while in summer it is register the high value for NOx and SO2 which reached 0.406ppm for NOx and 0.319 for SO2 while TSP recorded difference reach high concentration 323.54 μg/m3 during Spring .While ,there is no difference has been recorded for black carbon in Autumn and Winter where as high concentration reached 25000 ng/m3 .The yearly difference in Al ashar shows that the highest for CO2 and CO during the period of studying followed by Al - Qurna recorded the highest concentration for NOx and SO2 while al - Zubair station recorded the highest concentration for TSP . Abu al Khaseb station recorded the lowest concentration for all gases and TSP because nature of the region which is formed from farms with lack of human activity .according to AQI which is use to measuringBthe air quality which shows the status of air and the degree of pollution , seasonally it is the highest rang for moderate in yellow color in Abu Khaseb station to unhealthy for sensitive groups in orange color in both al Zubair and Altenuma station where as al Qurna station recorded red color for unhealthy and in Al Ashar station they are record very unhealthy in purple color .while AQI for annually difference for the stations of study for unhealthy for sensitive groups in orange color for all stations except Abu Khaseb station , these groups are considered by negative affection human being health .Biochemical analysis has been done for leaves of plants that are spreading in the study area for knowing the effect of pollutants on biochemical indicators .The study included three kinds of plants which are phoenix dactylifera , ziziphus sp. And conocarpus lancifolius . Biochemical analysis test included total chlorophyll , water content, ascorbic acid and pH .the total chlorophyll estimated by using UV - spectrophotometer ,while ascorbic acid was estimated by using gas chromatography . The results shows significant different among leaves for different plants species in different study stations , the Date palm trees phoenix dactylifera recorded the highest concentration for total chlorophyll with the comparison with other plants. The seasonally differences show that lowest concentration of total chlorophyll in Winter reached 2.11 mg/g .The ascorbic acid recorded highest value in Winter reached 1.41 mg/ g and the water content rate of less recorded value in Spring reached %59.26 with no differences are recorded in the rest of the seasons . pH was recorded the highest rang in Winter reached 7.53.Ziziphus sp. recorded the lowest content of total chlorophyll for Winter reached 1.87 mg/ g and ascorbic acid recorded the highest value in Winter 1.31 mg/ g . whereas there was no significant different in water content in all seasons .pH recorded the high rang 6.64 in Winter , there were no significant in the rest of the season .Conocarpus lancifolius recorded the lowest value of total chlorophyll comparison with the other plants .while the lowest value for total chlorophyll recorded 1.42 mg/g in Winter . Ascorbic acid reached the highest concentration 1.31 mg/ g in Winter , while the water content recorded the lowest value in Winter 36 .88 % . While pH recorded 5.80 the lowest value in Winter.According to the biochemical analysis for plants for getting the value APTI for knowing the ability of tolerance of plants for air pollution which reflects the capability of the plants to tolerance of air pollution . The result of the study shows that Phoenix dactylifera recorded the highest value 7.36 in Al ashar station where as there are no significant recorded among the other station . Ziziphus sp. recorded the highest value 6.41 in al - Qurna station followed by al - Ashar station 6.07 whereas the other station did not recorded any significant . It has been noticed that Conocarpus lancifolius shows that lowest value of APTI which reached 3.91 in Al Zubair station and there were significant differences among the other stations.APTI was used for getting the value of Anticipated Pollution Index (API) which estimating biological characteristic and the economic value for plants. The results of API showed that the plants in Basra province Phoenix dactylifera recorded the value 6 and it was in excellent category. Ziziphus sp. recorded as poor plants recorded the value 2 . while Conocarpus lancifolius recorded the value 1 category as very poor plants.it has been noticed from values of APTI and API for phoenix dactylifera makes it typical plants as bioindicator and reducing the pollution .It is concluded for the current study that there are variation quantity and quality for gases and obvious spread for it is concentration of polluted gases and particles in air of the study stations in Basra. It was found that Phoenix dactylifera is a typical plants for using as bioindicator and reducing the air pollution .

عزل وتشخيص اميبا Acanthamoeba castellani ودراسة تاثيرها الممرض في الفئران المختبرية Mus musculus سلالة Balb/C == Isolation and Detection of Acanthamoeba castellani. and study their pathological effect on laboratory mice Mus musculus Balb \C strain

Author name: شيماء عبد الرزاق حميد
Supervisor name: مسلم عبد الرحمن محمد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: free_living species of amoeba belongs to the genus Acanthamoeba was recorded in the recent study , this species was classified as A. castellani was according to the morphological features , cultural properties , heat tolerance and osmosis .Astromoeba was also recorded that characterized by its star - shape and Naegleria spp. which isolated from environmental samples ,both are free living amoeba .Acanthamoeba was isolated from different environments , includings water and soil from various regions of Basrah Provinace and from gathered water in air - cooler basin and discarded water from air conditioning systems .It was isolated from the eyes of out - patients who were suffered from eye problemes and from healthy volunteers.Several culture media were used for parasite culturing including media S, Agar, PYG, Mix , Mud , Heat - killed E.coli coated agar ,Lock and Manitol media.The media differ in their capability for the parasite growth depending on their nutrients ingrediants and the suitable temperature for the growth ,and the high rate for the growth was on S medium 37◦C and there is increasing in the parasite size that it is a new medium and used for the first time .The PYG medium at 37◦C also cause a good growth for the parasite .The parasite susceptibility to Dexonium was examined in vitro which shows the activity of the drug against the parasite .The activity of other drugs including Cisplatin , Pentostam , Chloromphenicol , Methadin , Cifran and Genedin was examined in this study .Cifran and Cisplatin shows a close effect and less than others ,while Methadin does not affect on the parasite ,in other hand Chloramphenicol caused obvious malformation in the parasite and retardation in the numbers.The recent study showed the death dose for half of the lab mice infected with the parasite was 450 amoeba/ml.The study also proved that the parasite is one of the major causes for Blinding ,causes it invades the mice eyes and blinds them moreover it cause skin lesions , hypertrophy in spleen and kidneys and bleeding in brain and lungs .Different ways used in the infecting of mice by the parasite caused the death of them in two - four dayes ,The chronic infection cause death in long period.The parasite can infect the brain when it is injected in different ways through optic nerve ,peritonium ,veins , nose and skin ,in which it caused necrosis .The presence of the trophozoite and cyst cause WBC infeltration.The liver ,kidneys and lungs were also infected when the parasite injected through the optic nerve , peritonium ,veins ,nose and skin and caused necrosis , bleeding patches , gathering of the defence cells (WBC).

تقدير بعض العوامل البيئية والبكتريولوجية لمياه الصرف الصحي في محافظة البصرة / العراق == Assessment of some environmental and bacteriological factors of Wastewater in Basra Province, Iraq

Author name: سهى محمد علي محمد سعيد المظفر
Supervisor name: منال محمد اكبر | ميثم ايوب عبد القادر
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية لمعرفة تاثير مياه الصرف الصحي على المحتوى البكتيري في محطة حمدان لمعالجة مياه الصرف الصحي /البصرة حيث تستلم المحطة مياه المجاري المنقولة عبر شبكة من الانابيب التي تستلم مياه المجاري من50% من مناطق مدينة البصرة. اختبرت ثلاث مواقع لاخذ العينات هي المحطة الاولى حوض التجميع الخارجي والمحطة الثانية حوض الترسيب الابتدائي اما المحطة الثالثة فهي المياه الخارجة من المحطة والتي تصب في شط البصرة.وشملت الدراسة ايضا قياس بعض الخصائص الفيزيائية والكيميائية لمياه المجاري وهي (الاس الهيدروجيني pH ودرجة الحرارة Temperature والملوحة Salinity والمتطلب الحيوي للاوكسجين BOD5 والفوسفات والمواد الصلبة العالقة الكلية TSS والمواد الصلبة الذائبة الكلية TDS والنتريت NO2 والنترات NO3) خلال الفترة من كانون الاول2014/ ولغاية ايار2015/.ويتضح من نتائج هذه الدراسة ان المتطلب الحيوي للاوكسجين BOD5 هو عامل مهم وله علاقة باعداد البكتريا الكلية الموجودة في مياه الصرف الصحي.كما شملت حساب كثافة المجاميع البكتيرية المتواجدة في مياه المحطات.ولحساب العدد الكلي للمستعمرات البكتيرية اخذت عينات مياه الصرف الصحي وزرعت على اوساط عامة وانتخابية وتفريقية مختلفة استخدم وسط الاكار المغذي Nutrient Agarكما واستخدم وسط المانيتول الملحي الانتخابي Mannitol Salt Agarلعزل المكورات العنقودية الذهبية Staphylococcusaureus ووسط اكار الماكونكي MacConkey Agar لعزل بكتريا القولون البرازية Escherichiacoli ووسط AgarXLD الانتخابي التفريقي (Xylose lysine deoxycholate Agar ) لعزل انواع البكتريا التابعة لجنس السالمونيلا Salmonella.تم ايضا دراسة حساسية العزلات تجاه المضادات الحيوية باستخدام طريقة الانتشار عبر الاقراص Disc Diffusion Test حسب طريقة كربي باور Kirby Bauer Method. ان دراسة حساسية عزلات E. coli توصلت الى ان العزلات البكتيرية ابدت نسب مقاومة عالية لاغلب المضادات الحيوية المختبرة في هذه الدراسة ، كذلك ابدت المكورات العنقودية الذهبية مقاومة عالية لاغلب المضادات الحيوية في حين كانت حساسة لكل من كلورامفينيكول (C) وسيبروفلوكساسين (CIP) ونورفلوكساسين (NOR) وكانامايسين (K). كانت عزلات السالمونيلا متباينة في حساسيتها تجاه المضادات الحيوية المختبرة في هذه الدراسة لكن ابدت عدم مقاومة او مقاومة واطئة للمضاد الحيوي CIP وNOR . ابدت المجاميع البكتيرية المختلفة قيد الدراسة انماط مقاومة مختلفة تجاه المضادات الحيوية اذ ابدت انماط مقاومة مختلفة. وكذلك ابدت العزلات البكتيرية المعزولة من مياه الصرف الصحي مستويات مختلفة من التحمل لتراكيز المعادن الثقيلة ،فيما يخص عزلات E. coliابدت العزلات مستوى عال من التحمل للنيكل بلغ 64% من العزلات كان MIC لها 4mg/Lوللكادميوم فان نسبة 93% من العزلات كان MICلها 0.8mg/L. اما بالنسبة للمكورات العنقودية الذهبية فان 89% من العزلات كان MIC لها 2mg/L لعنصر النيكلوبالنسبة للكادميوم فان 33% من العزلات كان MIC لها 0.4mg/L .وبالنسبة للسالمونيلا كان تحمل 100% من العزلات لعنصر النيكل هو عند 2mg/L في حين كان 50% من العزلات MIC لها 4mg/L كحد اقصى لعنصر الكادميوم. | The present study has been conducted to detect the effect of different parameters of sewage on microbial content at Hammdan for wastewater treatment in Basra city. The station receive sewage transported via net of pipes from different parts of Basra city .Three sites in the station for taking samples ,the first site is the external collection basin, the second site is primary sedimentation basin, the third site is water output that carry the water after treatment to flow at Shatt al Basra. Some characteristics of sewage were measured, these include pH, Temperature, Salinity, BOD5, TDS, TSS, Phosphate, NO2 and NO3 from period December/2014 to May/2015. In this study it is clear that BOD5 is an important factor related to total bacterial count in sewage. The bacteriological study of sewage has been done including measurement of total count of bacteria isolation and counting of Escherichia coli, Staphylococcus aureus and Salmonella spp. From different stations selective media is used for isolation including Nutrient Agar , MacConkey Agar , Mannitol salt Agar and XLD Agar . The antibiotic susceptibility of the isolates towards selected antibiotics have been studied using disc diffusion test. In the present study E. coli isolates showed high percentage of resistance to most of antibiotics. Staphylococcus aureus also showed high percentage resistance to most of antibiotics studied and found susceptibility to Chloramphenicol, Ciprofloxacin and Kanamycin. Variable suceptibility to tested antibiotics in the present study has been shown by Salmonella spp., Salmonella spp. were susceptible or show low resistance to Ciprofloxacin and Norfloxacin .Different Antibiotics Resistance Profiles has been shown by different bacteria groups studies. The bacterial isolates from sewage in this study have shown different levels of tolerance to different heavy metals (Nickel and Cadmium). E. coli tolerate high concentration for Nickel (%64) and for Cadmium (%93). High tolerance level for Cadmium has been shown for Salmonella spp (%50).

خصائص خمائر البيئة النهرية لمحافظة البصرة وقابلية بعضها على المعالجة الحيوية للعناصر الثقيلة والهيدروكربونات النفطية == Characterization of yeasts in river habitat in Basrah Province and an ability of some of them for Bioremediation of the heavy metals and oil hydro carbonate

Author name: سناء قاسم بدر
Supervisor name: باسل عبد الزهرة عباس
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:

تقييم حالة النباتات المائية الغاطسة في بعض مناطق هور شرق الحمار وشط العرب جنوبي العراق باستخدام بعض دلائل التنوع الاحيائي == Assessment of the Status of Submerged Aquatic Plants in Some Areas of East Hammar Marsh and Shatt Al - Arab River Southern Iraq Using Some Biodiversity Indices

Author name: سما عباس عبد المجيد الزيدي
Supervisor name: دنيا علي حسين العباوي
General topic: Biology
Specific topic: Aquatic Environment
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted to assess the state of submerged aquatic plants in some areas of East Hammar marsh and Shatt al - Arab River, as measured by some of the chemical and physical properties of water and sediment in six stations, three of which are located in East Hammar marsh (Nagara, Sallal and Almshab) and the other three are located in the Shatt al - Arab (the island Muhammadiyah, Salhiya and Mhella). Environmental surveys of submerged aquatic plants were done and registered their presence, their vegetation cover and biomass as well as the biodiversity indices (diversity and evenness, and dominance) for 12 months from October 2015 until September 2016.The air temperature in the East Hammar marsh ranged between 19 - 45.3 C, water temperature 14.3 - 32.4C , transparency between 20 - 100cm , Turbidity between 20.9 - 65.3 NTU, depth of 50 - 160 cm and dissolved oxygen between 5.4 - 6.9 mg / L, pH between 7.6 - 8.6 , electrical conductivity between 3.8 - 12.5 mS / cm, nitrates between102.23 - 549.64 μg/L, effective phosphorus between 4.15 - 9.6 μg/L . As for the Shatt al - Arab River values ranged : air temperature between 18 - 48 C , water temperature was between 14.2 - 32.3 , Transparency between 23 - 90 cm, turbidity between 12.8 - 68.3 NTU , depth of 60 - 200 cm , dissolved oxygen between 5.4 - 7.9 mg / L , pH between 7.5 - 8.51 , electrical conductivity between 2.8 - 12.4 mS / cm , nitrates between 106.17 - 569.42 μg/L, effective phosphorus between 4.93 - 9.13 μg /L .BpH and electrical conductivity values of the sediments in the Shatt al - Arab and East Hammar marsh were reached 7.7 - 8.3 and 1.08 - 2.91 mS / cm and 7.5 - 8.4 and 1.12 to 3.05 mS / cm respectively, effective phosphorus values in sediment ranged in sediment of East Hammar marsh between 5.69 - 42.51μg/L, While in Shatt al - Arab fluctuated between 5.12 - 40.3 μg/L, and the value of the organic matter in the Shatt al - Arab were 5.6 to 14.5%, while in the East Hammar marsh reached 7.4 - 15%. The sediments are similar texture in the Shatt al - Arab and East Hammar marsh (Silty clay).The presence of three species were recorded in the Shatt al - Arab River included Potamogeton crispus , Potamogeton pectinatus and Potamogeton perfoliatus, while five species in the East Hammar marsh Ceratophyllum demersum, Myriophyllun spicatum , Potamogeton crispus , Potamogeton pectinatus and Potamogeton perfoliatus.As calculated biodiversity evidence was that East Hammar marsh was more diverse than the Shatt al - Arab, while dominancy was clear in the Shatt al - Arab, compared with East Hammar marsh.This study showed that the sudden rise in salinity values as well as increased nutrients led to degradation of water quality, which was reflected on the presence and vegetation cover of submerged aquatic plants as well as biodiversity resulting from the presence of Tilapia and other fish that use submerged aquatic plants as food as well as the human factor.

تاثير هرمون الثايروكسين على بعض المعايير الوظيفية للدم وبعض الجينات المنظمة لتمايز النسيج العظمي في الفئران المختبريةmusculus L. Mus. == Effect of Thyroxine hormone on some of physiological parameters of blood and some regular genes of bone differentiation in Laboratory Mice Mus musculus L.

Author name: سلمى سعيد عباس
Supervisor name: سامي جبر المالكي | علي عبد اللطيف العلي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية لمعرفة تاثير هرمون الثايروكسين على تمايز العظم وبعض المعايير الفسلجية والتي شملت المعايير الدموية والهرمونات المحرضة للقند واوزان واطوال الاجنة واوزان المواليد في الفئران المختبرية البيضاء Mus musculus L.,اذ قسمت اناث الفئران المختبرية في الدراسة الحالية على مجموعتين هما : المجموعة الاولى الرئيسية : لدراسة تاثير هرمون الثايروكسين L.Thyroxine hormone على المعايير الدموية للاناث الحوامل وغير الحوامل وعلى تمايز العظم في الاجنة(باستخدام طريقة تحضير المقاطع النسجية الاعتيادية للاجنة والطريقة الجزيئية باستخلاص المادة الوراثية (RNA) من الاجنة وباستخدام تقنية النسخ العكسي RT - PCR والبلمرة الكمية qRT - PCR ومعرفة تاثيره على اوزان المواليد والاجنة, وتالفت من 90 انثى بالغة بعمر(10 - 12)اسبوع وقسمت على مجموعتين ثانوية ,و هي مجموعة السيطرة والتي حقنت حيواناتها بـ 0.1 مل من المحلول الفسيولوجي0.9% ولمدة 10 ايام متتالية.بينما المجموعة المعاملة حقنت حيواناتها بـ0.1 مل من هرمون الثايروكسين وبجرعة مقدارها 0.5 ملغم /كغم/يوم ولمدة 10 ايام يوميا بعدها شرحت 5 اناث من كلا المجموعتين ,و زوجت 40 انثى المتبقية ولكلا المجموعتين مع الاستمرار بالحقن لحين تشريح الاناث واستخراج الاجنة خلال فترات عمرية متباينة (9و10و11و12و13و14و15) يوم.اما المجموعة الثانية الرئيسية خصصت لدراسة تاثير هرمون الثايروكسين على الهرمونات المحرضة للقند وهرمونات الدرقية وانزيم Alkaline phosphatase.وتتالف من 15 انثى وقسمت على ثلاث مجاميع ثانوية تضم كل منها (5) اناث, وهذه المجاميع هي : مجموعة السيطرة وحقنت بـ0.1 مل من المحلول الفسيولوجي 0.9%.ومجموعة المعاملة الاولى وحقنت بـ0.1 مل من محلول هرمون الثايروكسين وبجرعة 0.5 ملغ/كغم/يوميا ولمدة 15 يوم للاناث العذارى قبل التزاوج.ومجموعة المعاملة الثانية حقنت بـ0.1 مل من محلول هرمون الثايروكسين وبجرعة 0.5 ملغ/كغم/يوميا ولمدة 30 يوم . اظهرت نتائج الدراسة الحالية ان المعايير الدموية قد تباينت قيمها خلال الفترات الزمنية وللمجموعات ضمن المدى الطبيعي عدا في حالات قليلة ,اذ وجد هناك انخفاض معنوي في اعداد خلايا الدم البيض والخلايا الحبيبية والخلايا الوحيدة والخلايا اللمفية والنسب المئوية للخلايا الحبيبية والوحيدة واللمفية واعداد كريات الدم الحمر,وكذلك قيم مستوى كمية الهيموكلوبينHb والنسب المئوية لحجم الدم المضغوط PCV(HCT)% ومعدل كمية الهيموكلوبين MCHومعدل تركيز الهيموكلوبينMCHC ومعدل حجم كريات الدم الحمرMCV والصفيحات الدموية PLT في فترات متباينة من الحمل للمجموعة المعاملة مقارنة مع مجموعة السيطرة. وبينت النتائج وجود زيادة معنوية في خلايا الدم البيض والخلايا الحبيبية والخلايا الوحيدة والخلايا اللمفية والنسب المئوية للخلايا الحبيبية والوحيدة واللمفية, ومعدل تركيز الهيموكلوبينMCHC والنسبة المئوية لحجم الدم المضغوط %HCT ومعدل حجم كريات الدم الحمر MCVو الصفيحات الدميةPLT في مدد متباينة من الحمل للمجموعة المعاملة مقارنة مع مجموعة السيطرة. وسجلت نتائج الدراسة الحالية حصول ارتفاع معنوي في قيم هرموني T4,T3في الحيوانات المعاملة مع انخفاض معنوي في تركيز هرمون TSHفي الاناث المعاملة مقارنة مع السيطرة,و اوضحت النتائج ايضا انخفاضا معنويا في مستوى الهرمون اللوتيني LH وهرمون الحليب وللمجموعتين(15و30) يوم وزيادة معنوية في الهرمون المحفز للجريبات FSHللمجموعة الثانية(30 يوما) ,مقارنة مع مجموعة السيطرة, ولايوجد تاثير معنوي لهرمون الثايروكسين على هرمون الشحمون الخصوي ولنفس الفترتين مقارنة مع مجموعة السيطرة, وحصلت زيادة معنوية في مستوى انزيم الفوسفاتيز القاعدي بعد 30 يوما من الحقن بهرمون الثايروكسين مقارنة مع مجموعة السيطرة ,وحصول انخفاض في اوزان الاجنة بعمر 15يوما والمواليد للاناث المعاملة مقارنة مع مجموعة السيطرة , وبينت الدراسة زيادة معنوية في اطوال الاجنة المعاملة بعمر 9 و10و11و15يوما مقارنة مع مجموعة السيطرة. اما الدراسة النسجية فقد بينت ان الهيكل العظمي في الاجنة بعمر 9و10و11 يوما عبارة عن بداءات اعضاء تتكون من نسيج ميزنكيمي,ولم تسجل الدراسة النسجية وجود فرق بين مجموعة السيطرة والمعاملة في تمايز العظم .وظهرت بداية التعظم الغشائي والغضروفي في الاجنة بعمر12يوما ,اذ لوحظ بداية تمايز الخلايا الميزنكيمية الى ارومات عظمية,مع وجود القليل من المادة البينية الشبيهة بالعظم بين الارومات العظمية في منطقة الفك السفلي قرب غضروف ميكل ,والفك العلوي والعظم الحنكي واللامي وغضاريف الاضلاع ,اما في الاجنة بعمر13 و14و15يوما فقد ازدادت عمليات تمايز العظم بنوعية ,فاضافة الى النمو الذي حصل في العظام السابقة في تكوينها فقد ظهرت عظام جديدة في مناطق اخرى من هيكل الجنين تمثلت بعظام الصفيحة القاعدية والفخذ وحزام الكتف وحزام الحوض والاضلاع ,اما النتائج الجزيئية الخاصة بتمايز النسيج العظمي في اجنة الفئران المختبرية فقد اشارت الى التعبير الجيني لبعض جينات النسيج العظمي وتشمل Runx2وOSX وOsteo(OSC) وSialo(BSP) والتي بلغت ذروتها في مرحلة (13) يوم. ان لهرمون الثايروكسين تاثير على التعبير الجيني لبعض الجينات التي ترتبط مع التمايز النسجي للعظم اذ يؤدي هذا الى التغير في التعبير الجيني زيادة احيانا وانخفاضا خلال المدد العمرية المختلفة للاجنة .نستنتج من الدراسة الحالية ان زيادة مستوى هرمون الثايروكسين يسبب اضطراب في المعايير الدموية والتعبير الجيني لجينات العظم وانخفاض مستوى الهرمونات المحرضة للقند (LH وProlactin) وزيادة مستوى الهرمون المحفز للجريبات, وتعد هذه الدراسة الاولى على مستوى جامعة البصرة | the present study was performed to know the effect of thyroxine hormone on differentiation of bone tissue and some of physiological parameters, which included blood parameters and Gonadotropine hormones and Weights and Lengths and birth weight of mus musculus L.the females are divided into two groups ;the main first group which included 90 adult females (10 - 12)week, that subjected to study effect of thyroxine hormone on blood parameters of pregnant , nonpregnant females ,and bone differentiation of embryos (by using tissue sections method and molecular technique by RT PCR , and qRT PCR), and also known it is effect on weight of fetus and newborns; this group was also subdivided into two secondary subgroup : - control group ; was injected with 0.1 ml of normal saline (0.9%) for 10 consecutive days ,and treated group; was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 10 consecutive days ,after that, 5 females from both groups were anatomized,while 40 females were mated of the both groups with continuous of injection until the females were subjected to anatomical practices and then the the embryo were taken during varied peroids (9,10,11,12,13,14,15) day. The main second group which included 15 females ,was selected to study the effect of thyroxine hormone on ;sexual hormone ,thyroid hormone and alkaline phosphatase . this group was divided into another 3 subgroups,one regarded as control group which ; was injected with 0.1 ml of normal saline (0.9%) for 15 consecutive days.and second the first treated group (T1) ; was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 15 consecutive days ,and without mating of the females,and the second treated group ; (T2);was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 30 consecutive days and also without mating of the females. Results of the present study were shown that the blood parameters were varied in value between periods and groups (decreasing and increasing among normal value ) with exception in few cases , significant decrease of W.B.C , the granular cells ,monocytes, lymphocytes ,lymphocytes percentage ,granulocytes percentage and monocytes percentage during different periods of pregnancy in the treated animals compared with control , and was decreased RBC ,Hb ,HCT percentage ,MCH ,MHCH ,MCV, and PLT in varied periods , and we noticed a significant increase in WBC ,granular cells ,monocytes ,lymphocytes , granulocytes percentage , monocytes percentage , MCHC ,HCT percentage,MCV, and PLT in varied periods of pregnancy in the treated animals compared with control. Our study was exhibited significant increase in levels of T3 and T4 hormones of the treated animals compared with control, with significant decrease in TSH of treated animals , also significant decrease in LH and prolactin H and for groups(15 and 30 days) ,and significant increasing in FSH of second group (30 day) ,when compared with the control , and there was no effect of thyroxine on TEST H level and for 2 periods(15 and 30) , significant increasing in alkaline pho after 30 days from injection with TH H , decreased of body weight of embryos (15 days) and newborns of the treated females ,the current study showed significant increase of lengths of treated embryos (9,10,11,15 days). The histological study was explained the skeletal tissue ossification of embryos during (9,10,11)day post gestation was primitive organs of mesenchymal tissue and The histological study was not show asignificant differences of bone differentiation between treated and control groups,and intramemberaneous ossification was appeared of embryos at 12 days ,in this stage the begining of differentiation of mesenchyme cells was appeared into osteoblast , with a little of matrix like bone between osteoblasts in Mandible near the meckle cartilage , Maxilla, Palatine,and Hyoid, and endochondral ossification as in Ribs, where in embryos (13,14,and 15) The bone differentiation processes were increased of bone (the intramembranous ossification and endochondral ossification),in addition to the growth of previously formed bones,also new bones were appeared in different areas in embryo skeleton like Basal plate,femur,Scapula girdle , pelvis girdle and the Ribs . In other hand, the molecular results of bone differentiation in embryo ,were exhibited peak of expression of some of the genes of bone tissue (Runx2 ,OSX, Osteo(OSC) ,and Sialo(BSP) ) at 13 days of embryo age, also the thyroxine hormone was effected in expression of some the bone genes that included disturbanceas like increasing of expression during peroids and decreasing in other peroids of embryos life. We concluded from the present study that the increase of thyroxine hormone level caused disturbances of blood parameters and gene expression of the bone genes & decreasing of the level of LH & prolactin hormones and increase the level of FSH. This is the first study on the Basrah University

تقييم فعالية عقاري Nandrolone decanuate وAlbendazole للاصابات المختلفة بداء الاكياس العدرية في الفئران المختبرية سلالة Balb/c == Evaluate the effectiveness of a drug Nandrolone decanuate and Albendazole for injuries the different infections by hydatid cysts in mice Balb/c

Author name: سعيد فاضل مناتي الحمداني
Supervisor name: مسلم عبد الرحمن محمد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: تبنت الدراسة الحالية استعمال طرق مختلفة لاحداث الاصابة برؤيسات الاولية Protoscolex المستخرجة من الطور اليرقي ( الاكياس العدرية ) لطفيلي Echinococcus granulosus وهذه الطرق هي الحقن الوريدي والجنبي وتحت الجلد وكذلك عن طريق الغشاء البريتوني . اظهرت الدراسة حدوث الاصابة وتطور الاكياس العدرية في كل الطرق اعلاه . كذلك تم استعمال طريقة الحقن في اللفائفي والتجريع عن طريق الفم لكن لم تظهر الاصابة المستخدمة بهذه الطرق اي تطور للاكياس العدرية في حيواتات التجربة . كذلك حقن 650 رؤيس اولي في جميع طرق الحقن وتبين حدوث الاصابة في نفس مواقع الحقن بطريقة الحقن تحت الجلد والحقن الجنبي وعن طريق الغشاء البريتوني. ولكن طريقة الحقن الوريدي اظهرت حدوث الاصابة وتطور الاكياس العدرية في اماكن مختلفة من جسم الحيوان المختبري ويكون الكيس العدري متموضعا بين نسيج العضو المصاب في حين الحقن عن طريق الجنب وتحت الجلد والغشاء البريتوني يكون الاكياس العدرية المتطورة ملتصقة على سطح العضو المصاب . كذلك اثبتت الدراسة وجود اختلاف في الوان الاكياس العدرية المتكونه وحسب طريقة الحقن. استـــــعمل في هذه الدراســــــــة عقار Albendazole وعــــــقار Nandrolone decanuate كعقارات علاجات للاصابة المستعملة وتبين ان لها تاثير ادى الى موت الرؤيسات الاولية بتقدم فترة الاصابة واثبتت الدراسة ان عقار Nandrolone اكثر كفاءة من عقار البندازول وذلك من خلال الاختلافات في النسبة المئوية لموت الرؤيسات الاولية وكذلك بسبب هذا العقارشوه في شكل الرؤيسات وطريقة ابعاجها اذ كان الانبعاج الى الخارج في حين كان الانبعاج الى الداخل في الاصابات المعالجة بعقار البندازول . | The present study showed various methods for the infection by the protoscolex obtained from the larval phase of the Echinococcus granulosus . which involved the venus,pleural,subcutaneous and peritoneal injection . In all injection method 650 protoscolex were injected ,It was found that no infection happen in the same location to the injection which resulted by the subcutaneous,pleural and pretonal injections. On the contrary to thatresulted by venous injection in wich the injection occurred in varoes sites of the lab animals body ,and the hydatid cysts were pervasine with in the diseased infected organ tissues. Wile that resulted by pertonial ,subcutaneous and hered the surface of the organ. The colors of the hydrated cysts were also varied according to the infection methods.Albendazole and Nandrolone were used as treatments against the induced infections and it was found that Nandrolone was more efficient than albendazole,That was observed by the rates of the protoscolex caused by the action of the drugs and desmorphorphology and invagination outward while in albendazole was inward

دراسة مقارنة لتاثير هرمون النمو في عضلات وCyprinus مناسل سمكتي الكارب الاعتيادي والبلطي احمر البطن carpio (L. 1758) Tilapia zillii (Gervais, 1848 == A Comparative Study to the Effect of Growth Hormone on Muscles and Gonads of Carp Cyprinus carpio (L. 1758) and Tilapia Tilapia zillii (Gervais, 1848)

Author name: سعد محمد صالح عبد الصمد
Supervisor name: عقيل جميل منصور | عبد الكريم طاهر يسر
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: In the present study, the effect of human growth hormone on the muscles and gonads of common carp Cyprinus carpio (L. 1758) and red belly tilapia Tilapia zillii (Gervais, 1848) was studied by : - 1 - Studying the effect of human growth hormone on the growth of the two studied species by intraperitoneal (IP) injection of three replicates of control with 120 μg of normal saline / g Body weight and two doses (low and high) with 60 and 120 μg of human growth hormone / g Body weight respectively for both species, then changes were followed up in each treatment after every two weeks of injection by : - a - Pursue histological changes in the muscular tissue by using Cell Profiler software for the first time in Iraq. b - Pursue changes in chemical components ratios of body muscles.c - Measuring of specific growth rate and length - weight relationship for fishes.2 - Studying of human growth hormone effect on the gonads of fishes (adult males and females) with 60 males for each species and a same number of females, all of them injected either with low or high dose with 60 or 120 μg of human growth hormone / g Body weight respectively for both species or with 120 μg of normal saline / g Body weight in fishes of control. This experiment had achieved in a month.3 - Knowing the direct effect of human growth hormone on the follicles by implementing an in vitro experiment with the follicles of carp which implanted in basic salt solution (BBS) then growth hormone was added to the test tubes with two concentrations 5 μg/ ml and 10 μg/ ml, the control follicles were implanted in normal saline. The results of the study revealed that the maximum rate of percentages of the muscle fibers with area less than 314 μm2 for region R1(Dorsal side) was 88.29 % in carp in high dose treatment after the eighth week of injection, while it was 88.00 % for tilapia in high dose treatment after the eighth week of injection, too.In the region R2 (in tail) the maximum rate of percentages of muscle fibers with area less than 314 μm2 was recorded after the eighth week from the beginning of injection was 73.3553 % for carp in high dose treatment and 85.935% for tilapia in high dose treatment.The results showed that 1.00984 and 1.000074 were the highest and the lowest values of condition factor for carp respectively. For tilapia those were 1.009993 and 1.000151 the highest and the lowest values of it respectively.The rate of specific growth rate for length was 0.3616 and 0.44866 cm/day% for carp and tilapia respectively. While the specific growth rates for weight were higher than the comparable one for length and were 1.3489 g/day% for carp and 1.6085 g/day% for tilapia.The results exhibited that the rates of protein percentages for both species in the all treatments in general tend to be higher with progressing of experimental time. Muscle water content percentage rate varied with difference of dose and experimental time. The maximum rate of fat percentage in both species was in control treatment after two weeks of hormone administration.The highest rate of carp male Gonadosomatic index was 6.55% in high dose treatment after the third week of injection, as for tilapia males was 6.103% in low dose treatment after the third week of injection, while the lowest value for this index was recorded after the first week of injection in high dose treatment which as 2.81 and 2.89% for carp and tilapia respectively. For females the highest rate of Gonadosomatic index was in high dose treatment after the third week of injection for both species which was 11.001 and 6.0624% for carp and tilapia respectively. As for the lowest rate of carp female Gonadosomatic index was recorded in control treatment after the first week of injection it was 5.54%, while the lowest rate of this index for tilapia females was 2.99% in low dose treatment after the first week of injection.In the experiment of direct effect of this hormone on the follicles of carp it was found that the highest value of polarization index was 4.12 which recorded in high dose treatment after 48 hours of incubation while it reached the lowest value which was 3.031 in high dose treatment after 24 hours ofincubation.

دراسة تشخيصية مظهرية وجزيئية للشريطيات المتطفلة على نورس مستدق المنقار Larus genei والكرسوع Himantopus himantopus شمال محافظة البصرة == Diagnostic, Morphological and Molecular Studies on some cestodes parasitized on Larus genei and Himantopus himantopus , North of Basrah province

Author name: سحر محمد علي محمد سعيد المظفر
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: During the period from August 2015 to July 2016, fifty three aquatic birds of Larus genei (slender - billed Gull) family Laridae and 52 of Himantopus himantopus family Recurvirostridae were collected from Al - masehab marsh north of Basrah Province.The current study was focused on these two birds due to its economic importance to the local people of the study area. These two birds were found infected with 10 species of cestodes. Echinocotyle longirostris and Infula burhini were recorded for the first time in Iraq. Larus genei was considered as a new host of Wardium fusa.The isolated cestodes were described and compared with other recorded species at different parts of Iraq and the world.The percentage and the mean intensity of infection of these 10 species of cestodes were counted monthly and seasonly together with single, double and trible infection during the whole year. The main percentage infection was reached to 95.2% and the main intensity was 13.49. In L. genei the percentage and mean intensity were 90.5% and 15.4 recepectively, while for H. himantopus were 100% and 11.8 recpectively. In L. genei the high percentage infection was found in August 2015 and September , November, and December 2016, and during March, April, May, June and July for H. himantopus. A significant difference was recorded between the percentage and the mean intensity of infection.Random Amplified Polymorphic DNA (RAPD) technique with random 6 primers was used to distinguish between Infula burhini and Acoleus vaginatus wich isolated from the bird H. himantopus due to the morphological similarities.The Scanning Electron Microscope (Pharmacy College, Basrah University) was used to photograph the scolex of A. vaginatus and I. burhini.

تشخيص وحياتية حشرة مع تصنيف الديدان الخيطية المتطفلة عليهافي محافظة ميسان == Diagnosis and life cycle insect Gryllotalpa gryllotalpa (L.) with a taxonomy parasitic nematodes in the province of Missan

Author name: سجى عماد نعيم الساعدي
Supervisor name: باسم هاشم عبد الله | ضياء خليف كريم
General topic: Biology
Specific topic: Zoology
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: It was collected 318 mole cricket insects randomly from four regions in Missan including AL Kahala district by 191 insects, AL Mishraah by 62 insects, Kalat Salih by 61 insects and New Hay AL Hussein ( Centre of the city) by four insects. The period was from September 2013 to August 2014. It was diagnosed one species of mole crickets which is G. gryllotalpa decended from Gryllotalpidae families because of its essential charact ristics such as the general form of the insect, shape of the wings and legs, and its site and colour. In addition, there is a large lobe in the front legs of the insect and there one two species of veins in the front wings of the male used to attract females for mating. The life cycle of the insect was studied in the laboratory. Itstransformation is characterized by being imperfect progress, on egg, then a nymph and then an adult. The female lays about 100 eggs oval - shaped and grey in a room in the soil. The period of preegg - laying is approximately 100 days and the percentage of hatchling is 74% . The females remain alive after one day egg - laying is completed while the males remain 13 days after egg - laying. The eggs hatch into nymphs which pass stages of life development andit takes 115 day - Newly hatched nymphs are transparent and grey, its length is 10 mm with antennas and small cerci which are without wings. Wings begin to appear in the sixth stage of life. Then nymphs develop into brown adults with a large filled body. Its length is about 40 mm and all parts of the insect continues to grow in proportion to the size of the insect.After examining these insect, it showed that they are infected with seven species of nematode parasites. They are Binema ornate, Binema korsakowi, Binema anulinervus, Cameronia triovata, Cameronia multiovata, Mirzaiella asiatica, Grylophilla skrjabini. The Prevalence of those nematodes are 18%, 13%, 1%, 2%, 1.0%, 22% and 25.6% respectively. while the mean intensity of infection was 1.5, 1.6, 3.5, 4.5, 8, 1.5,2.15 respectively.Four of these species of nematodes were considered as new record in Iraq, These are : Binema anulinervus : Most important feature in the shape of the high - tail and long that look like a flagella.Mirzaiella asiatica : Featuring male this form of the parasite by the short and broad and meandering tail. The female is characterized by a length of the muscle of the esophagus and the existence of the tapes curled at his side. Cameronia triovata : The most important feature of this parasite egg shaped oval that is flat from one side. C. multiovata : What also distinguishes this form of the parasite is a large and long oval egg.Nematodes were a taxonomically quoited and illustrated.The species B. ornate and G. skrjabini appear repeatedly over 10 months followed by the parasite B. korsakowi for eight months then followed by M. asiatica for seven months, as for the parasites B. anulinervus, C. triovata, C. multiovata for two months

دراسة مظهرية وجزيئية بتقنية RAPD لبعض الاصناف الغيبانية لنخيل التمر في البصرة == Morphological and Molecular Study by RAPD Technique for Some Un Known (Ghaibany)Cultivars date - palme In Basrah

Author name: زينب شعلان عبد الرزاق علي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية اثناء الموسم الزراعي للعام 2015م على احد عشر صنفا بذريا (غيباني) لنخيل التمر Phoenix dactylifera L. النامية في البصرة وهي اصناف جديدة تسجل لاول مرة, درست ثمان وعشرون صفة من صفات النمو الخضري, وست وعشرون صفة من الصفات الثمرية كما. درست صفات البذور, فتبين ان الاصناف المدروسة اختلفت في الخواص الخضرية والثمرية, اذ اظهرت الدراسة ان كلا الصنفين نبوي والصنف نجمة تميزا بشكل التاج الشبه منفتح في حين كانت الاصناف الباقية مضمومة التاج. وكذلك انفرد الصنف نجمة عن باقي الاصناف بلون الاوراق الاخضر الشمعي. وتراوح معدل طول الورقة من 2.68 م في صنف الوردية الى 4.35م في الصنف نمروشة, كما تراوح معدل طول منطقة الاشواك من 56 سم في صنف دكلة عبد الى 160سم في الصنف ام اللسين. وتباينت الاصناف في معدل عدد الاشواك فكان ما بين 18 شوكة في الصنف وردية الى 47 شوكة في الصنف ام اللسين. واختلفت الاصناف بنمط ترتيب الاشواك على المحور الوسطي فقد انفرد الصنف برماني بنمط توزيع الاشواك الثنائي وامتاز الصنف وردية بالنمط المفرد الثنائي في حين كان النمط المفرد هو السائد في الاصناف الباقية, واتضح من الدراسة ان النسبة المئوية لطول منطقة الاشواك الى طول الورقة الكلي هي من الصفات التي تتسم بالثبات دونا عن باقي الصفات الخضرية. اما بالنسبة للخواص الثمرية فقد تراوح معدل طول العرجون للاصناف المدروسة مابين 66 سم في الصنف رحماني الى 152سم في الصنف برماني والذي يعد من العراجين الطويلة, وانفرد الصنف دكلة داود بلون العرجون الاصفر المائل للوردي اما الصنفين برماني ونجمة فقد كان لون العراجين فيهما اخضر في حين كان لون العراجين في الصنفين وردية ونبوي اصفر يميل للاخضرار واصفر في باقي الاصناف. اما الخواص الفيزيائية المدروسة للثمار فقد سجل الصنف دكل داود اقل المعدلات في حجم الثمرة في مرحلتي الخلال والتمر والتي كانت 5سم3 و2.6سم3 على التوالي, وامتاز الصنف الزريجي بكبر حجم ثماره لكلا المرحلتين الخلال والتمر 17سم3 و10.24سم3 على التوالي, وتبين ان طعم الخلال في الاصناف وردية ودكلة عبد والصنف رحماني والصنف زريجي عفصي اما الاصناف الباقية فهي حلوة المذاق. وامتاز الصنف نجمة بكونه مبكر جدا في موعد النضج وكانت الاصناف الباقية مبكرة النضج في حين كان الصنف برماني متوسط النضج, وامتازت اصناف الدراسة بكون التمور فيها طرية القوام عدا الصنف رحماني الذي كان قوام التمر فيه شبه جاف. واستخدمت الدراسة برنامج التحليل الالي لتحليل المكونات الرئيسة للثمار في مرحلة الخلال, فتبين ان الاصناف انقسمت بمجموعتين وحسب التقارب في البيانات المغذاة للبرنامج فانعزل الصنف الزريجي بمجموعة مستقلة لكون بياناته متفردة, اما المجموعة الثانية فقد توزعت الاصناف فيها الى تحت مجموعة, صغرى ضمت الاصناف دكلة داود ونجمة واقترب منهما الصنف وردية, اما الاصناف الباقية فتوزعت هي الاخرى بتحت مجموعتين. استخدمت الدراسة الجزيئية تقانة PCR RAPD لتثبيت البصمة الوراثية للاصناف المدروسة ولكشف الارتباط الوراثي بينها, وجرى باختبار عشرة بادئات عشوائية من مجموعة OPA وبادئان من مجموعة OPB. تم تحليل نواتج التفاعلات للبادئات مع Genomic DNA الاصناف كافة وكل على حده لتحديد مدى كفات البادئ فكانت النتيجة ان اربعة من البادئات ( OPA0.2 وOPA0.3 وOPA0.5 وOPA0.6) نجحت في كشف التباين بين الاصناف المدروسة, وبينت نتائج التحليل العنقودي للنواتج التفاعلات للبادئات ان الاصناف توزعت بمجموعتين, صغرى ضمت كل من الاصناف زريجي ونمروشة والصنف ام اللسين ومجموعة كبرى ضمت الاصناف الباقية, وقد توزعت فيها الاصناف الى تحت مجموعة ثانوية. مما يشير الى الاصول المشتركة, وبينت الدراسة امكانية انتخاب اصناف بذرية تمتاز بخصائص ثمرية جيدة وتكثيرها لدعم الاقتصاد الوطني. | This study was performed during agronomic season in 2015 on 11 cultivars (ghaibany) Phoenix dactylifera L. that grow in basrah, and these cultivars are new recorder. Twenty eight characteristics of vegetative growth were studied, seed characteristics were studied also. It was concluded that the studied cultivars varied in there vegetative and fruit qualities. The study showed that both cv Nabawy and cv Najma distinguished by semi - opened crown but the other cultivars had closed crown . In addition, cv Najma marked by waxen green leaves. The rate of leaf length ranged from 2.68 m in. cv Wardiah to 4.35m in cv Nammrosha. besides the length of spine area ranged from 56 cm in cv Deglat Abd cultivars to 160 cm in cv Am - Ellsian. The cultivars also varied in the rate of spine number which was 18 in cv Wardiah an 47 in cv Am - Ellsian. The cultivars differed in the style of spine arrangement on rachis since cv Bermany distinguished by the dual style of spine arrangement, while in cv Wardiah marked by single dual style, however the single style was prevalent in other cultivars. The study clarified that the percentage between spine area to the total length of the leaf was one of qualities that characterized by stability that differed from other vegetative characteristics. As for fruit characteristics, the length of peduncle of cultivars concerned ranged from 66 in cv Rahmany cultivars to 152 in Bermany and that was regarded long peduncle. cv Deglat Dawood marked by colour of peduncle which was yellow look like rosy, whereas cv Bermany and cv Najma the colour of their peduncle were yellow look like green and yellow in the rest. As for physical characteristics of studied fruit, cv Deglat Dawood recorded the lowest rates in the volume of fruit during the phases of khalal and dates, the volume was 5 c.m and 2.6 c.m respectively, cv Zraigai distinguished by the bigness of the volume of the fruit for both phases of khalal and date 17c.m and 10.24 c.m. The taste of khalal in the cultivars of cv Deglat Abd, cv Wardiah, Rahmany, cv Zreigai was tannic while the taste of other cultivars were sweet. cv Najma cultivars distinguished by being so early in maturity, the other cultivars were early in maturity but cv Bermany was middle in maturity. The dates of studied cultivars were soft except cv Rahmany that was semi - dry. The study used a program of automatic analysis to analyze the main components of fruit in khalal phases the, it became clear that the cultivars divided into two groups according to the convergence data that fed to the program. cv Zraigai cultivars was isolated in independent group because its data were unique, but the second group ,cultivars in which were classified in small group included cultivars of cv Deglat Dawood and cv Najma and cv Wardiah approached to this group. The other cultivars classified into two groups.The molecular study used P C R RAPD technique to fix genetic print of studied cultivars and to discover the genetic link among them. Ten of random primers of OPA group were tested and two of OPB were tested too. The products of reaction of primers were analyzed Genomic DNA for cultivars totally and individually; to determine the efficiency of primer the results was that for of primers succeeded in discovering the variation among cultivars concerned. The result of cluster analysis of reaction products of primers showed that the cultivars divided into two groups : small group included cv Zairgai, cv Nammrosha and cv Am - Ellsian cultivars, and big group contained the other cultivars in which cultivars distributed into secondary group and that refer to the common origin. The study showed the ability of electing and increasing Ghaibany cultivars that marked by good characteristics to support national economy

دراسة تصنيفية وحياتية للفطريات الناقصة الملونة في البصرة == Taxonomical and Biological study of Dematiaceous imperfect - fungi in Basrah

Author name: زينب خلف عبد الله
Supervisor name: توفيق محمد محسن
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: During this study 23 species belong to 10 genera of imperfect fungi were isolated from different natural sources ( plants, soil , and ari) from Basrah govermanete using different isolation methods.Among the isolated fungi the genus Alternaria was selected to identify its species by using the morphological characteristis by light microscopy and Scaning Electron in Microscope (SEM) and verified by molecular and chemical methods. Eight species of Alternaria were identified namely; A.alternata (isolate1 and 2) A.chlamydospora A.citri, A.raphani , A.longipes , A.infectoria A.tenuissima and A.chrysanthemi based on light microscope examination.Taxonomic relegation of these species were confirmed by SEM image. SEM revealed that there was differences in the conidial wall ornamentation .The molecular analysis using PCR depending on the loci ITS1, and ITS4 700bp which confirmed the morphological identification of most of species namely A.alternata (isolate 1 and 2) , A.chlamydospora , A.longipes , A.infectroia and A.tenuissima. However , A.citri was related to A.alternata, A.raphani was belonged to A.infectoria and A.chrysanthemi is belonging to A.lolii. Nevertheless ,by using the loci OPA1 - 3 there was no variation among the species of Alternaria ,this is may be due to the inadequate method applied.The genetic variation of 36 isolates of A.alternata was studied using 9 - primers by RAPD - PCR technique. All primes gave an amplification of DNA for all the fungal isolates. Among the primers OPC08 exhibited a highest efficacy reaching 235 total bands .In the present study the antibacterial activity of fungal crude extract was tested. It appeared that the extract exhibit an antibacterial bioactivity against E.coli , S.aureus , S.epidermidis and S.saprophyticus.However, the extract of A.alternata (isolate 1 and 2) showed an inhibition growth against all the tested bacterial strains. This study was also revealed that the fungal filtrates of Alternaria species possessed various chemical compounds. However, there were differences in the chemical composition among the fungal filtrates.The results showed that Alternaria species ( A.alternata isolate 1 and 2, A.chlamydospora A.citri, A.raphani , A.longipes) were capable to biosynthesize silver nanoparticles, when the fungal filtrate were treated with AgNO3 solution after72 hrs incubation .Silver nanoparticles synthesis was checked by the change of colour from yellow to brown and confirmed by Uv - vis spectrophotometry at wave length (420, 440 nm). SEM image showed that the silver nanoparticles with spherical shape and size ranged between (20 - 72nm). Aconclusion can be derived that application of SEM,chemical analysis of fungi extracts ,molecular analysis and the silver nanoparticles characteristics are useful in the taxonomy of fungi to solve the overlapping of the morphological identification .Taxonomic key were prposed for the species of the genes Alternaria based on the morphology , chemical compound with the fungal extracts and size of silver nanoparticles synthesized by fungi .Melanin pigment identify by TEM microscope and it appeare like dark drop inside the cell .
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