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دراسة مصلية ومناعية لداء المقوسات في مرضى القلب == Serological and Immunological Study of Toxoplasmosis in Heart Disease Patients

اسم المؤلف: حيدر صباح عبد الحسين
اسم المشرف: بسعاد عقرب العبودي | احمد حسن محمد
الموضوع العام: علوم الحياة
السنة: 2017
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: ذي قار
الصفحات الاولى:
المستخلص: Toxoplasmosis is the general term for infection and disease in human and animal caused by a parasite called Toxoplasma gondii, this disease is prevalent among different categories of people , Heart disease patients are one of them. Because of the dormancy status of T. gondii, there is ambiguity and variation in the immunity of patients. This study aimed to investigate the immune status of heart disease patients infected with toxoplasmosis, to detect the prospective relationship between heart disease patients and T. gondii, and investigate the relationship between toxoplasmosis and risk factors which included gender, age, blood group, contact with cats, and habitation.One hundred fifty samples of blood had been collected at period from August 2016 till November 2016 from patients with heart disease, they lying or visit Heart Medical Centre in Thi - Qar province. According to gender, the patients divide in to (81 males and 69 females) with age ranging 18 to 82 years. Also one hundred samples of blood had been collected from apparently healthy persons as negative control ,dividing according to gender in to ( 55 males and 45 females) with age ranging 19 to 56 years . All samples of both subjects had been tested by ELISA technique to detect anti - Toxoplasma Abs (IgG & IgM) . While the positive samples with toxoplasmosis were tested to detect the level of interleukins (IL - 10, IL - 12 and IL - 17).The overall of seropositivity of anti - Toxoplasma Abs that scored by ELISA in the current study was 44% in heart disease patients are higher than those in apparently healthy controls 19%, and there was a significant difference between them. There is a significant difference between male and female, in the seropositivity of anti - Toxoplasma Abs that scored in controls, in male was higher than those in female. Presence of antiSummary toxoplasmosis Abs in all types of heart disease patients with significant difference among them, and the highest percentage of anti - toxoplasmosis Abs in Mitral stenosis( MS) was 55.8% , and the lower value was 30.3% in Myocardiopathy.There are significant differences between the age groups and the percentage of anti - Toxoplasma Abs in heart disease patients with highest value was 63.2% at age group of 61 - 75 years, and the lowest one was 26.3% at age group of 15 - 30 years. While no significant differences between the age groups and toxoplasmosis in apparently healthy controls with highest value was 19.5% at age group of 31 - 45 years, and the lowest one was 8.6% at age group of 15 - 30 years.The results demonstrated that seroprevalence of toxoplasmosis was significantly associated with close contact with cats. The percentage of seroprevalence anti - Toxoplasma Abs in heart disease patients and controls inhabited rural area was 52.5% and 32% are higher than those inhabited urban area was 34.3% and 6% respectively. Some blood parameters were estimated of both subjects infected and non - infected with toxoplasmosis. There was high significant difference at p≤0.05 observed in mean ratio of eosinophil’s number between infected and not infected subjects with toxoplasmosis in both subjects. While there was no significant differences in mean ratio of total count of WBCs. Moreover, mean of lymphocytes number recorded significant differences betweeninfected and non - infected heart disease patients only. The blood group phenotypes of the infected heart disease patients and controls highlight the role in toxoplasmosis infection and the AB blood group characterized by the highest significant percentage of infection. The results of interleukins appeared high mean levels of IL - 12 82.85 pg/ml in heart disease patients infected with toxoplasmosis when compared with control 60.97 pg/ml, and there was significant difference between them. While there was no significant difference in IL - 10 and IL - 17 in both patients and controls. The results also showed decreased level of IL - 17 in patients with heart disease. The increased level of IL - 12 in patient with heart disease infected with toxoplasmosis indicate to the activity of cell mediated immunity in those patients and may be lead to provoke an inflammatory status in the heart valves and muscle then worse heart disease to be sever and need complicated treatments

عزل وتشخيص المضاد الحيوي المنتج بواسطة عزلة Penicillium chrysogenum من البيئات المختلفة في ترب مدينة الناصرية == Isolation and Identification of antibiotic produced by Penicillium chrysogenum isolated from different environments in Nasseriya city soi

اسم المؤلف: ميسم كريم حسن
اسم المشرف: سعد سلمان هميم | محمد حسين مشهد
الموضوع العام: علوم الحياة
السنة: 2017
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: ذي قار
الصفحات الاولى:
المستخلص: The present study includes the isolation and identification of some mycoflora from 40 soil samples in six places ( Remnants of fat - born, parks, edges of the river, animal wastes, sewage and rubbish) during October 2015 - January 2016 in Nasseriya City, Iraq. According to different environmental factors, the isolated genera were Aspergillus, Penicillium, Mucor, Rhizopus, Cladosporium, Sepedonium chrysospermum, Alternaria chlamydospora, Bipolaris, Chrysosporium, Candida albicans, Rhododendron flavum, Humicola, Geotrichum candidum, Fusarium and Acremonium. Three isolation methods were used. Dilution method, direct plate method, alcohol and heat treatment technique using the cultural media viz. Potato Dextrose Agar ( PDA), Sabouraud Dextrose Agar (SDA) and Potato Carrots Agar (PCA). Aspergillus represented the highest fungal isolates which represent 62 (37.12%). followed by Penicillium with 47 (28.14%), Mucor 22 (13.16%), Rhizopus 15 (8.98%), Cladosporium 6 (3.59%), Sepedonium chrysospermum and Alternaria chlamydospora, 3 (1.80%), Biopolaris, Chrysosporium and Candida albicans with 2 (1.20%), and finally Rhododendron flavum, Humicola, Acremonium, Fusarium and Geotrichum candidum recorded the lowest fungal isolation with one isolate for each (0.60%). The study was aimed to isolation of Penicillium from soil and assay its antibacterial activity. The results showed that dilution method gave a best fungal growth in comparison with direct plate method and alcohol and heat treatment technique in 25 ºC and pH = 6. PDA appeared as an optimum medium for isolation in comparison with other media such as SDA and PCA. The preliminary results showed that Penicillium sp. exerted antibacterial activity against Gram positive and Gram negative bacteria; Therefore, this fungus was used for production of antibiotic on Potato Dextrose Broth (PDB) medium. The optimum conditions were obtained at pH = 6, incubation temperature of 25 ºC and shaking rate of 180 rpm for 7 days to fermentation. Ethyl acetate was a good organic solvent to extraction of antibiotic which produced (3g/L) as white to creamy crystals. The characterization of the antibiotic product after extraction and purified by chemical methods included Thin Layer Chromatography (TLC) test, Nuclear Magnetic Resonance (NMR) Spectra and Mass Spectra. The Minimum Inhibitory Concentration (MIC) for clinical bacterial species was 1 - 10mg/ml.The PCR method used in this study utilized Internal transcribed spacer (ITS1 - 4) as a primer for identification of isolates. Two PCR products of the targeted gene of Penicillium chrysogenum isolates which locally named as (Penicillium chrysogenum F1 and Penicillium chrysogenum F2) were selected and subjected to partial DNA sequencing for the ITS gene to follow up the possible molecular relationship between these local isolates and what recorded globally in Genbank.

تاثير داء السكري وعقار البنتوستام على تطور الاصابة بداء اللشمانيا الاحشائية في ذكور الفئران المختبرية == The Impact of diabetes and Pentostam on development the infected Leishmania donovani parasite in male laboratory mice

اسم المؤلف: افراح علي عبد الامير العزاوي
اسم المشرف: فاضل عباس منشد العبادي
الموضوع العام: علوم الحياة
السنة: 2017
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ذي قار
الصفحات الاولى:
المستخلص: This study included evaluation of the role of diabetes and Sodium Stibogluconate drug (Pentostam) against infection leishmaniasis parasite in laboratory animals experimentally infected with the parasite, Where we used in this study male white laboratory rats of the strain Mus musculus Balb /c. Aggregates were divided randomly into eight major groups of eight mice, The first group were injected with saline solution at a dosage of 0.2 ml, the group was considered as a negative control, The second group were injected with parasite (1.2 × 10 6 parasites / 0.2 ml) at a dosage of 0.2 ml, The third group was injected with the parasite and alloxan at a dosage of 0.2 ml, while the fourth group were injected only with alloxan, While the fifth group were injected with a Pentostam drug at a dosage of 20 mg/kg, as well as the sixth group were injected with leishmania parasite at a dosage of 0.2 ml and Pentostam drug at a dosage of 20 mg/kg while the seventh group were injected with the parasite and alloxan at a dosage of 0.2 ml and Pentostam drug at a dosage of 20 mg/kg while the eighth group injected with alloxan at a dosage of 0.2 ml and Sodium Stibogluconate drug at a dosage of 20 mg/kg, Where it was dissected first four groups a period of 45 days while the other groups were dissected after 75 days with recorded weights of the body before and after the experiment. Also studied the histological pathological changes and taking weights for each of the liver and spleen, blood samples were taken for the purpose of knowing the effect of which occurred in the blood parameters and the level of glucose in the blood. The results of the statistical analysis of the current study showed in the second set significantly higher (P≤0.05) total weight of the body and the weight of the liver spleen, White blood cells lymph and acidophilus, while there was a significant decrease in the total number of white blood cells, neutrophils and red blood cells there was no significant difference (P≤0.05) inthe blood glucose concentration compared with the first group. The fourth group showed a significant decrease (P≤0.05) in total body weight and red blood corpuscles with (P≤0.05) blood glucose level and neutrophil cells, the groups treated with the drug showed the sixth and seventh group height body weight and white blood cells and lymphocytes and a significant decrease (P≤0.05) red blood cells, neutrophils and acidity. There was no significant difference (P≤0.05) in the concentration of glucose in seventh group. The eighth group showed a significant decrease (P≤0.05) in the total number of white and red blood cells and significant increase (P≤0.05) in lymphocytes and acid, while there was no significant difference (P≤0.05) concentration of blood glucose. The fifth group showed the treatment of the drug loss in the radiology of hepatic cells and degeneration of clear and congestion in the central vein with the infiltration of inflammatory cells within the hepatic tissue, and showed a clear exhaustion in the white pulp and the proliferation of red pulp.we observed in the histological sections of the present study in the sixth group infected with parasite and treated with pentostam, a significant improvement of the liver tissue. The spleen showed a simple propagation of the white heart. The seventh group is experimentally infected with leishmania and the alloxan The development of diabetes and the treatment of pentostam is spread with Kupffer cells and expansion in the blood sinusoids with changes in the spleen. White and the group of nematodes around it with breeding in the red pulp as in the fifth group.

التقييم المتوقع للعوامل الاحيائية الدقيقة والفسلجية لتطور التدرن البشري في محافظة ذي قار == Prospective Evaluation Of Microbiological And Physiological Factors Of Human Tuberculosis In Thi-Qar Province/Iraq

اسم المؤلف: نافع فرحان مفتن الفرحان
اسم المشرف: منال بادي التميمي | خالد كاطع الفرطوسي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: ذي قار
الصفحات الاولى:

المحتوى البايوكيميائي لبعض الطحالب الدقيقة وتاثير بعض العوامل البيئية والملوثات في الاحماض الدهنية غير المشبعة == Biochemical Content of Some Microalgae and Effect of Some Environmental Factors and Pollutants In Unsaturated Fatty Acids

اسم المؤلف: صفاء حسين عبد الله الحسيناوي
اسم المشرف: حسين يوسف الركابي | راجي طعمة الزاملي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ذي قار
الصفحات الاولى:

الكشف عن بعض جينبت الضراوة في بكتريب Bacillus cereus المعزولة من بيئبة مختلفة في محافظة ذي قار == Detection Of Some Virulence Genes In Bacillus Cereus Isolated From Different Sources In Thi-Qar Province

اسم المؤلف: اسراء ظاهر طاهر
اسم المشرف: راجي طعمة ناصر | منال بادي صالح
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: ذي قار
الصفحات الاولى:

دراسة مناعية وكيموحيوية لاضداد الدهون المفسفره في النساء المجهضات == The Immunological And Biochemical Study For Antiphospholipid Antibody In Women With Recurrent Miscarriage

اسم المؤلف: لمى رشيد لفتة الخزاعي
اسم المشرف: منال بادي التميمي | محمد عجه عوده
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: ذي قار
الصفحات الاولى:

دراسة عن بعض المسببات الميكروبية للاجهاض والمعاير الدموية للنساء المجهضات == study of some microbial causes of abortion and hematological parameters of aborted women

اسم المؤلف: اسيل زغير سعدون الحميداوي
اسم المشرف: قاسم حسن وداعه | طالب فالح حسن
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ذي قار
الصفحات الاولى:

السمية الخلوية للسيميتيدين و فالبروات الصوديوم المؤثرة على خطوط الخلايا السرطانية == Cytotoxic Effect Of Cimetidine And Sodium Valproate On Cancer Cell Lines

اسم المؤلف: مسلم محسن خلف
اسم المشرف: علي اسماعيل السنافي | ناهي يوسف ياسين
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: ذي قار
الصفحات الاولى:

دراسة نوعية وكمية للطحالب القاعية وعلاقتها بالمحتوى العضوي في رواسب نهر الفرات عند مدينة الناصرية == Qualitative and Quantitative Study of benthic Algae and relation with Organic Content of the Sediment in the Euphrates River at Al- Nasryia

اسم المؤلف: نور عبد الكريم محسن المياحي
اسم المشرف: باسم يوسف الخفاجي | حسين يوسف الركابي
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: البيئة المائية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ذي قار
الصفحات الاولى: