التحري الجزيئي عن النمط الثالث لداء السكري البادئ عند النضج (MODY 3) في مرضى السكري العراقيين == Molecular Investigation of Maturity Onset Diabetes of The Young Type 3 (MODY 3) In Iraqi Diabetic Patients
التحري عن التشوهات الكروموسومية وطفرات المورث FLT3/ITD في مرضى ابيضاض الدم النخاعي الحاد == Screening For Chromosomal Aberrations And Gene Mutations FLT3/ITD In Patients With Acute Myeloid Leukemia
التنميط الوراثي لمستضدات التطابق النسيجي في عينة عراقية من مرضى السكري النوع الثاني == Hla Genotyping In A Sample of Iraqi Type 2 Diabetes Mellitus Patients
التقييم الجزيئي لنسخ جين المقاومة الدوائية MDR1 في بعض المرضى العراقيين البالغين المصابين بسرطان ابيضاض الدم الحاد == Molecullar Assessment of Multidrug Resistance Gene (MDR1) Transcript In Some Adult Iraqi Patients With Acute Leukemia
دراسة جزيئية عن جين المقاومة mecA في بكتريا العنقودية الذهبية المقاومة للمشيسلين والمعزولة من بعض مستشفيات بغداد == Molecular Study For Detection of Meca Gene In Methicillin - Resistant Staphylococcus Aureus Isolated From Some Hospital In Baghdad City
الكشف الجزيئي عن التغيرات في جين MSX1 المسؤول عن حالة فقدان الاسنان باستخدام سلسة تفاعل البلمرة في عينة من المرضى العراقيين == Molecular Detection of Msx1 Gene Changes Responsible For Causing Hypodontia Using Polymerase Chain Reaction (PCR) In Sample of Iraqi Patients
Author name:
اماني احسان الصقر
Supervisor name:
اسماعيل حسين عزيز | اكرم فيصل الحويزي
Abstract:
مصطلح الهايبودونشيا يعني نقص الاسنان الخلقي ويعد من اكثر التشوهات الفموية حدوثا لدى الانسان. مائة وخمسة وسبعون من مرضى نقص الاسنان الخلقي سجلوا في هذه الدراسة لديهم على الاقل سن واحد مفقود ولايزيد عدد الاسنان المفقودة عن ستة، قصدوعلاج الاسنان التقويمي في | Hypodontia means congenitally missing teeth, and considers as one of the most common oral alteration in human. One hundred and seventy five of hypodontia patients were matriculated in this study, having at least one missing tooth and no more than 6 missing teeth, seeking orthodontic treatment, who attended Al - Huwaizi Dental Center at AL - Harethia District - Baghdad, and control group consists of twenty five apparently healthy subjects (15 females, and 10 males). The age of both groups ranged from14 to 65 year. Buccal swabs were collected (for molecular study) from 25 of those patients who attended the clinic in a period between the beginnings of October 2013 to the end of April 2014, and from control group. Information were taken from the subjects under study according to a questionnaire that includes, name, gender, age, family and medical history, and the place of residence. Hypodontia was diagnosed according to the history of patients, clinical examination, orthopantomogram (OPG), and dental casts. The result of demographic study of hypodontia patients revealed that hypodontia was found in 129 of females which were more than that in males(46) with significant difference (p < 0.05). The ratio of females to males was 2.8 : 1. The missing teeth in hypodontia patients were found in right, left or both sides. The number of hypodontia patients, who had missing teeth in the right side were 37, in the left side were 48, while in both sides reached to 90 with non - significant differences and the results clarified that the missing teeth in anterior region found in 81 patients were higher than that in posterior region (63) while the least in both regions which recorded in 31 patient. It was found that hypodontia was more common in the maxilla (73) (upper jaw) than that in mandible (65) (lower jaw), whereas 37 suffering from missing teeth in both jaws, with non - significant differences. Present results showed that the maxillary lateral incisor (LI) was the most frequently missing tooth (124), the second most missing tooth was mandibular second premolar (PM2) (101) followed by lower central incisor (CI) (33), the maxillary second premolar(PM2) (27) whereas the lowest frequently missing teeth were canine (C), and the first premolar (PM1). The molecular part of present study used polymerease chain reaction (PCR) technique for amplification of DNA samples extracted from buccal swabs of twenty five hypodontia patients and control group.Four pairs of primers X1.1F, X1.3R; X1.4F, X1.4R; X2.1F, X2.3R, and X2.3F, X2.4R of the MSX1 gene, obtained from Bioneer Company (Korea), were used to amplify overlapping regions of the 2 exons of the MXS1 gene. The first pair of primers was used to amplify fragment with product size of 421 bp., while the second, third, and forth pairs of primers were used to amplify fragments with product size of 152 bp., 493 bp., and 264 bp., respectively. The outcome of MSX1 gene amplification showed that four patients with the first pair of primers and nineteen patient with the third pair of primers gave negative result (no bands) which differed from the result of the other patients and control. The disappearance of bands may be attributed to MSX1 microdeletion in those patients.The sequencing of MSX1 gene for the PCR product of second, third and fourth pairs of primer showed no genetic mutation, while the PCR product of the first pair of primers reveled nine missense and two silent mutations.It was concluded that hypodontia occurre frequently in Iraqi population and its occurrence in females was higher than that in males, and the gene MSX1 is responsible for many teeth missing in hypodontia patients.
التحري عن طفرة JAK2V617F والمستويات المصلية لانزيمي الفوسفتيز القلوي واللاكتيت ديهايدروجنيز في مرضى ابيضاض الدم النخاعي المزمن == Detection of Jak2V617F Mutation And Serum Levels of Alkaline Phosphatase And Lactate Dehydrogenase In Chronic Myelogenous Leukemia
تقييم التعبير الجيني للجينات CK19, MGB, MUC1 microRNA - 195 and microRNA - let 7a في نساء عراقيات مصابات بسرطان الثدي == Evaluation of MGB1, CK19, MUC1, microRNA - 195 and microRNA - let - 7a Expression In Iraqi Women With Breast Cancer
التحري الجزيئي عن بعض التغيرات في الدنا المايتوكونديري للنطف لمرضى يعانون من وهن حركة النطف == Molecular Screening of Some Changes In Sperm Mitochondrial DNA In Asthenozoospermic Patients
تشخيص بكتريا Neisseria gonorrhoeae بالطرق التقليدية والجزيئية في المرضى الذكور ودراسة مدى تاثيرها في حدوث الحذوف في موقع AZF == Conventional And Molecular Diagnosis of Neisseria Gonorrhoeae In Male Patients And Study Its Suspected Effects In Microdeletions In Azf Locus
دراسة بعض تاثيرات اللقاح المحضر من العزلة المحلية لبكتيريا Klebsiella pneumoniae == Study of Some Effects of Prepared Vaccine From Local Strain of The Klebsiella Pneumoniae
الكشف عن الرز المحور وراثيا باستخدام انواع مختلفة من التفاعل الانزيمي المتسلسل PCR == Detection of Genetically Modified Rice By Different Type of PCR
Author name:
ياسمين ابراهيم فرحان
Supervisor name:
امنة نعمة الثويني
Abstract:
In recent years, foods produced by genetic engineering technology have been on the world food markets. The biosafety aspects, regulations, and labeling for these foods are still contentious issues in most countries. Thus detection and quantificationof GMOs play crucial role for developing regulations on GM foods.In this study, eighty six non - labeled rice samples from different locals and exported market were analyzed to detect the genetic modification using a DNA based detectionvmethods as, conventional Polymerase chain reaction (PCR), and Real time PCR (RTPCR).The DNA rice samples were extracted by manual C - hexadecyl - Trimethyl - Ammonium - Bromide (CTAB) method and wizard kit method. The result revealed that DNA yield by the two methods is comparable. Rice DNA tends to be of a higher concentration when purified with the CTAB method; however, this particular DNA is more easily to amplify, the optical density (OD) was recorded 1.70 - 1.98 and the concentration of DNA quantified by fluorometer DNA rice samples, ranged from 11 to 50.5 ?g/?l. The DNA rice sample has also been used successfully with the Wizard Genomic DNA Purification Kit, and showed varieties in quality, the OD was recorded 1.65 - 1.95, and the concentration between 4.7 - 43.8 ?g/?l.The rice specific gene (sps gene) was detected by PCR. The results demonstrate that the purity of the extracted DNA in all tested rice samples was sufficiently high for a sensitive PCR analysis and the primer of detected gene appeared clearly at 251pb.Three genes; CaMV 35S promoter, NOS terminator, and insecticide resistant gene Cry1Ac were used to detect of GM rice by PCR, and Real time PCR using oligonucleotide sets targeting to novel genes. The result showed that there was no positive result reaction with conventional PCR, while the outcome of gradient PCR revealed a positive reaction in one sample (Uncle Bens brown) for CaMV35S promoter only. Gradient PCR with 12 replicons for each sample was used for qualitative detection of CaMV35S promoter gene, after optimization of melting temperature and cycles run (45 cycles) , the results appeared positive in the last three grades (63.9, 64.6, 64.9) for CaMV35S promoter, but NOS terminator, and CryIAc were recorded negative results.The result of Real - Time PCR clarified that the CaMV35S promoter specific primer showed strong amplification with Ct, and Tm values were reached into 33.73, 38.63 and 61.55, 62.92 in two samples Uncle Bens brown and Himalayan brown, respectively, whereas NOS terminator gave positive results in four samples Maxims, Laasturiana, Carolin white and Mahatma, and the values Ct and Tm reached to30.87, 30.31, 30.54, 33.75 and 64.53, 64.61, 62.62, 63.87 respectively in comparison with the positive control, while CryI Ac which did not show any positive signal.It was concluded that using molecular methods like Real - time PCR will be useful tool for detecting GM rice such as a part of the approval detection processes because of the rarity of data concerning consumption of GM rice in Iraq.
تقييم اختبارات PCR وطرق الزرع الاعتيادية في التشخيص المبكر لتجرثم الدم لدى الاطفال في مستشفى حماية الاطفال التعليمي في مدينة الطب / بغداد == Evaluation of PCR And Culture Methods For The Early Diagnosis of Bacteremia In Children From Welfare Teaching Hospital In Medicine City /Baghdad
