انتشار بكتريا Escherichia coli في المرضى في المرضى المصابين بالتهاب المرارة الحصوي في محافظة الديوانية == Dissemination of Escherichia Coli In Patients With Cholecystitis Calculus In Al - Diwanyia Governorate
التحري عن عوامل الضراوة لبكتيريا اشيريشيا القولون المعزولة من المرضى المصابين بالتهاب المجاري البولية وقياس نمط بعض الحركيات الخلوية لديهم == Detection of Virulence Factors of Escherichia Coli Bacteria Which Isolated From Patient With Urinary Tract Infection And Measuring Some Cytokines In Them
Author name:
رواء ماجد محمد البو صالح
Supervisor name:
ميثم غالي يوسف
Abstract:
تضمنت هذه الدراسة جمع 100 عينة من اعمار مختلفة للمرضى المصابين بالتهاب المجاري البولية الذين راجعوا مستشفى الديوانية التعليمي ومستشفى الولادة والاطفال في مدينة الديوانية , خلال المدة من تشرين الثاني 2012 الى نيسان 2013 للتحري عن بكتيريا Escherichia coli ا | groups of patient with pyelonephritis, whose visited Al - Dewaniya teaching and Woman s and children hospital in Al - Dewaniya city during the period from November 2012 to April 2013 for detection the isolates of Escherichia coli. The results showed that 56% of bacterial isolates were E.coli and 44% isolates belonged to others bacterial types. These results indicated that the females were more infected 67.86%(38\56) with pyelonephritis than the males 32.41(18\56). Always our result were pointed that the age of 30 - 39 old years have beeninfected with pyelonephritis and present the high percentage infection 30.36% comparison with others age groups (1 - 9, 10 - 19, 20 - 29, 30 - 39, 40 - 49, 50 - 59, 60 - 69, 70 - 79) , with percentage of (1.79%, 7.14%, 14.29%, 30.36%, 19.64%, 10.71%, 12.50%, 3.57%) respectively. The resistance of E.coli isolates to the four generation of Cephalosporin antibiotics were showed the following state : - The first generation of Cephalosporin antibiotic were indicated to Cephalothin 76.6%, Cefazolin 71.4%, Cephlexin 69.7% and Cephadroxil 66.1%. - The second generation of Cephalosporin antibiotic were indicated to Cefaclor 59.0%, Cefonicid 60.8%, Cefprozil 50.0%, Cefoxitin 64.2% and Cefmetazole 55.3%. - The third generation of Cephalosporin antibiotic were indicated to Ceftriaxone 32.2%, Cefotaxim 35.7%, Ceftazidim 37.5%, Cefixim 42.9%, Cefdinir 35.7% and Ceftizoxim 30.4%. - The fourth generation of Cephalosporin antibiotic were consisting of one antibiotic only like Cefepime with percentage of 39.3% The results concerned the virulence factors genes showed that the gene irp2 witch responsible for taking the iron from the blood, whereas the gene pap responsible for the production of P - type Pilli, afa gene responsible for the production fimbriae, iha gene responsible for production of capsule and the gene tst responsible for toxic shock. The result of PCR for the E.coli DNA showed that all the thirty isolates of this bacteria contain the gene irp2, while the others genes pap, afa, hly, and iha were presented the following percentage 36.6%, 30.0%, 96.6% and 10.0%respectively, whereas the gene tst didn’t recorded any isolates concerting. So in this study was to measure immune factors related to the urinary tract infection is in general and pyelonephritis in specially, has been measure some of cytokines which (Interleukin - 8, Interleukin - 6, Tumor necrosis factor - ?), they found increase in these factors in sera of patients with pyelonephritis compared to healthy persons.
التاثيرات النسيجية والدمية والكيموحيوية لفلوريد الصوديوم على بعض اعضاء الجسم في الارانب المحلية == Histological, Haematological And Biochemical Effects of Sodium Fluoride On Some Body Organs of Local Rabbits
تاثير مستخلص الثوم والرمان على الجرذان المصابة تجريبيا بطفيلي Cryptosporidium parvum ومقارنتها مع عقار Metranidazole == Effect Extract Allium Sativum And Punica Grantium On White Rat Expermental Infection Cryptosporidium Parvum And Comper With Metranidazole
دراسة بيئية للهائمات النباتية في مبزل CD4A (مبزل هور شعيب) الديوانية - العراق == Ecological Study of The Phytoplankton In Cd4A (Hor Shuaib) Drainage In Al - Diwaniya Iraq
انتشار انزيمات بيتالاكتاميز نوع OXA بين عزلات بكتيريا الزوائف الزنجارية في مدينة الديوانية == Prevalence of OXA Beta - Lactamaes of Pseudomonas Aeruginosa In Al - Diwaniya City
Author name:
رنا مشعل سالم
Supervisor name:
سيوف خومان علوان الرماحي
Abstract:
The aim of This study is to determine the prevalence of OXA ? - lactamase in pseudomonas aeruginosa isolates from clinical and other environmental cases in Al - Diwaniya Teaching Hospital using phenotypic and molecular methods.The Samples of the study were collected from various sources and they were 390 samples for the period from November 2011 to March 2012, and they includ (292 clinical specimens and 98 environmental sample), the results of cultural and biochemical tests showed that 50 isolates (39 of them were from clinical cases and 11 were from environmental samples), belonged to ps. aeruginosa, and their diagnosis were confirmed by 16s - ribosomal RNA, and the study showed that all the isolates containing the 16s - ribosomal RNA gene, which represents the designed diagnostic gene in this study.The results of this study showed that the higher rate of P. aeruginosa isolates was due to burns infections (23.68 %) , followed by respiratory tract infections (sputum) that were (15.38 %) and then the case to otitis media infections (12.5 %), and lastly that due to urinary tract infections (10.49 %), while the rate isolation from the environmental samples was (5.11 %) from the floor of burns department, followed by (10.8 %) from the medical instruments of department workers.It was noted from this study that the age and gender of the patients have some effect on the infection by P. aeruginosa it was recorded that the higher rate of infection was in patients older then 61 year old, and regarding gender, it was found that females are more infected than male, especially in urinary tract infections. The study showed that there is high rate of infection in the admitted patients, in older people and in those who need urinary catheterization, in addition to those with severe burns and those in the intensive care units. Drug sensitivity test had been carried out for all the bacterial isolates to 22 types of antibiotics by disk diffusion method of Kirby - Bauer, and this study had indicated that there was relatively high resistance from P. aeruginosa to ? - lactam antibiotics, aminoglycoseides, and fluoroquinolone. The results also showed that the studied isolates has possibility of producing broad - spectrum ? - lactames enzymes, and this is supported by the resistance of this bacteria to the third generation cephalosporins and to Alaztronam, it was also shown that there was difference in the resistance to carbapenems antibiotics, the resistance rate to imipenem and meropenem were 16% and 64 %, respectively. Polymyxin B and Polymyxin E also called (Colistin) antibiotics were also used, and the resistance rate %0 for Colistin and %4 for Polymyxin B, that means there was two isolates which are resistant to Polymyxin B and this resistance was a challenge to the success of therapeutic efforts. this study noted that there was 22 (44%) isolates which were resistant to at least three types of antibiotics, and regarded as multi - drug resistant (MDR) and Extensive - drug resistance (XDR) isolates were26 (52%) which was the higher rate among the three types of resistance, The third type of resistance was 2 (4%) which represents the resistance to all types of studied antibiotics (PDR). The ability of these isolates to produce broad - spectrum OXA ? - lactames enzymes groups was investigated thrrough detection of presence of genes blaOXA - 10, blaLCR - 1, blaOXA - 18, blaOXA - 1, blaOXA - 2 in these isolates by using the polymerization chain reaction enzyme technology] (PCR) it showed 50/50 (100%) isolates contain blaOXA - 10 gene, which belongs to the OXA group I, and the results of this study showed no amplification results for blaOXA - 18, blaLCR - 1, blaOXA - 1, blaOXA - 2 genes, which belong to the main groups of broadspectrum OXA ? - lactames enzymes which are OXAgroup II, OXAgroup III and OXA groupV respectively except OXA18 enzyme
