انتشار بكتريا Escherichia coli في المرضى في المرضى المصابين بالتهاب المرارة الحصوي في محافظة الديوانية == Dissemination of Escherichia Coli In Patients With Cholecystitis Calculus In Al - Diwanyia Governorate
التحري عن عوامل الضراوة لبكتيريا اشيريشيا القولون المعزولة من المرضى المصابين بالتهاب المجاري البولية وقياس نمط بعض الحركيات الخلوية لديهم == Detection of Virulence Factors of Escherichia Coli Bacteria Which Isolated From Patient With Urinary Tract Infection And Measuring Some Cytokines In Them
Author name:
رواء ماجد محمد البو صالح
Supervisor name:
ميثم غالي يوسف
Abstract:
تضمنت هذه الدراسة جمع 100 عينة من اعمار مختلفة للمرضى المصابين بالتهاب المجاري البولية الذين راجعوا مستشفى الديوانية التعليمي ومستشفى الولادة والاطفال في مدينة الديوانية , خلال المدة من تشرين الثاني 2012 الى نيسان 2013 للتحري عن بكتيريا Escherichia coli ا | groups of patient with pyelonephritis, whose visited Al - Dewaniya teaching and Woman s and children hospital in Al - Dewaniya city during the period from November 2012 to April 2013 for detection the isolates of Escherichia coli. The results showed that 56% of bacterial isolates were E.coli and 44% isolates belonged to others bacterial types. These results indicated that the females were more infected 67.86%(38\56) with pyelonephritis than the males 32.41(18\56). Always our result were pointed that the age of 30 - 39 old years have beeninfected with pyelonephritis and present the high percentage infection 30.36% comparison with others age groups (1 - 9, 10 - 19, 20 - 29, 30 - 39, 40 - 49, 50 - 59, 60 - 69, 70 - 79) , with percentage of (1.79%, 7.14%, 14.29%, 30.36%, 19.64%, 10.71%, 12.50%, 3.57%) respectively. The resistance of E.coli isolates to the four generation of Cephalosporin antibiotics were showed the following state : - The first generation of Cephalosporin antibiotic were indicated to Cephalothin 76.6%, Cefazolin 71.4%, Cephlexin 69.7% and Cephadroxil 66.1%. - The second generation of Cephalosporin antibiotic were indicated to Cefaclor 59.0%, Cefonicid 60.8%, Cefprozil 50.0%, Cefoxitin 64.2% and Cefmetazole 55.3%. - The third generation of Cephalosporin antibiotic were indicated to Ceftriaxone 32.2%, Cefotaxim 35.7%, Ceftazidim 37.5%, Cefixim 42.9%, Cefdinir 35.7% and Ceftizoxim 30.4%. - The fourth generation of Cephalosporin antibiotic were consisting of one antibiotic only like Cefepime with percentage of 39.3% The results concerned the virulence factors genes showed that the gene irp2 witch responsible for taking the iron from the blood, whereas the gene pap responsible for the production of P - type Pilli, afa gene responsible for the production fimbriae, iha gene responsible for production of capsule and the gene tst responsible for toxic shock. The result of PCR for the E.coli DNA showed that all the thirty isolates of this bacteria contain the gene irp2, while the others genes pap, afa, hly, and iha were presented the following percentage 36.6%, 30.0%, 96.6% and 10.0%respectively, whereas the gene tst didn’t recorded any isolates concerting. So in this study was to measure immune factors related to the urinary tract infection is in general and pyelonephritis in specially, has been measure some of cytokines which (Interleukin - 8, Interleukin - 6, Tumor necrosis factor - ?), they found increase in these factors in sera of patients with pyelonephritis compared to healthy persons.
