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انتشار المقاومة للمضادات الحيوية وبعض عوامل الضراوة في بكتريا Pseudomonas aeruginosa المعزولة من مستشفيات في النجف المعزولة من مستشفيات في النجف == Molecular Study To Some of Pseudomonas Aeruginosa Virulence Factors Isolated From Clinical And Environmental Isolates

Author name: ذو الفقار حيدر حسن
Supervisor name: ماجدة مالك متعب
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:
Abstract: اجريت الدراسة الحالية لغرض التحري عن وجود الجينات الكروموسومية المسؤولة عن بعض عوامل الضراوة المهمة لجنس Pseudomonas aeruginosa كانتاجها للسم الخارجي A والالجنيت اضافة الى الجينات الكروموسومية المسببة لمقاومة الكوينولونات في العزلات السريرية والبيئية لبكت | This study include investigate the presence of chromosomal quinolone resistance genes and virulence genes(toxA and algD) in clinical and environmental isolates from Al - Sadr Medical city.A total of 110 samples were collected from different clinical sources(burns, wounds, and urine), and 50 from the hospital environment.the samples were collected during 10, Oct, 2012 to 2, Feb, 2013. A total number of Pseudomonas aeruginosa isolates accounted for 89 of 160 samples.The distribution of these isolates were 28 from Burns, 14 wounds, 15 urine, and 32 from hospital environments. Identification of isolates has been accomplished depending on phenotypic diagnostic methods and PCR technique.our study proved that PCR technique depending on oprI and oprL genes was a rapid method for identification of non - pigmented isolates. A total of 20 antibiotics resistant isolates were chosen for Molecular studies. The result reveling that 100% of isolates had a chromosomal mutation in the QRDRs of genes (gyrA, gyrB, parC, and parE). High percentage of isolates(45%) carried mutated genes gyrA and parC, followed by 15% of isolates were carried either mutated gene gyrA alone or three mutated genes gyrA, parC, and parE and and 10%of isolates were carried three muted genes gyrA, gyrB, and parC, while gene that encoding to parC mutation were detected in 5% of isolates. Our study is the first study about detection of Quinolone Resistance Determining Regions in P.aeruginosa in Iraq. The results showed the the presence of mexAB - oprM multidrugs efflux system expression in 100%(20) isolates. while the phenotypic test depending on MIC values for ciprofloxacin alone in the presence of efflux inhibitor showed that only 15%(3) isolates had the active efflux pump.

تقييم مستوى بعض الحركيات الخلوية لدى المرضــى المصابين بفايروس الحلا البسيط النمط الاول Herpes simplex virus - 1 == Evaluation of The Level of Some Cytokines In Patients With Herpes Simplex Virus Type I (HSV - 1)

Author name: حسين علي كاظم
Supervisor name: سهام جاسم الكعبي
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:
Abstract: هدفت الدراسة الحالية الى تسليط الضوء على مجموعة من المرضى المصابين بفايروس الحلا البسيط النمط الاول Herpes simplex virus - 1(HSV - 1) من خلال تقييم مستوى نوعين من الحركيات الخلوية الموجودة في مصولهم. شملت هذه الحركيات الخلوية الانترلوكين Interleukin - 6(I | The present study aimed to highlight a group of patients infected with herpes simplex virus type I (HSV - 1) by evaluating levels of two types cytokines in sera. These included cytokines interleukin - 6 (IL - 6) and tumor necrosis factor - alpha (TNF - ?) has been evaluated using the technique of Enzyme - linked immunosorbent Assay (ELISA), has also been investigating the relationship between these cytokines and the stages of infection compared to the control group. Before that rapid cytologic method has been used to diagnose the virus by detecting the presence of multinucleated giant cells, and then was diagnosed serologically by specific IgM and IgG monoclonal antibodies against Herpes simplex virus - 1. It was also used Radial immunodiffusion method to evaluate levels of total IgM and IgG in patients sera.Sixty four sera samples were collected from patients who are attending to the consultants clinics of Al - Hakeem general hospital and AL - Sader teaching hospital in AL - Najaf AL - Ashraf governorate for the period from October 21/2012 until March 13/2013. These Samples were divided into two groups : (32) sample during primary and recurrent infection, (32) after recovery from infection (latent stage), as well as (16) sample were collected from healthy individuals, who were regarded as acontrol groups.The study reached to the following results : • A significant increment (p<0.05) in sera concentrations of IL - 6 in patients during infection as compared with control groups, but the increment was not significant in sera of patients during latent stage.• TNF - ? levels increased in sera of patient, but this increment was not significant as compared with controls group.• There was no significant increase of Total IgM levels in sera of patients.• Total IgG levels increased significantly in sera of patients during infection and latent stage as compared with control group.• A highly significant positive correlation (r= 0.7, p= 0.00) between TNF - ? and IL - 6 concentration in sera of patients.• There was no significant correlation (p>0.05) between Total IgM levels and (IL - 6 and TNF - ?) in sera of patients with correlation coefficient (r= 0.1, r=0.2) respectively.• Positive significant correlation (r= 0.4, p= 0.02) was found between total IgG levels and IL - 6 in patients during infection, but was no correlation between them in latent stage.• There was no association between age and IL - 6 and TNF - ? levels in sera of patients with HSV - 1.Finally, the elevation of TNF - ? and IL - 6 levels in sera of infected patients with HSV - 1 may be play an important role in pathogenicity of virus, as well as it may limits the viral infection.Furthermore, there are positive correlation between levels of TNF - ? and IL - 6 in sera of patients

دراسة جزيئية للـ Bacteriocine المنتج من قبل بكتريا lactobacillus salivarius == Molecular Study of Bacteriocine Produce By Lactobacillus Salivarius

Author name: ايمان حمزة محمد
Supervisor name: احلام نعيم كاظم الياسين
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:
Abstract: هدفت الدراسة الحالية التحري عن قابلية عزلات بكتريا Lactobacillus salivarius على انتاج البكتريوسين ودوره في تثبيط فعالية بعض العزلات البكتيرية السالبة لصبغة غرام. جمعت 155 عينة من مصادر سريرية مختلفة شملت 20عينة مسحات مهبلية، 8 عينة مسحات اللعاب، 6عينة حل | The aime of study detecting the a bility of Lactobacillus salivarius to produce bacteriocin and determine it’s role in inhibition the activity of some Gram negative bacteria. Atotal of 155 sample from different clinical source has been collected during the period from October - 2012 to March - 2013, these include 20 samples from vaginal smear, 8 samples from saliva and 6 samples from human milk.The results of primary isolation, Laboratory diagnosis and biochemical test showed that 34 isolates has bee identified as L.salivarius.Ahigh precent of isolation has been carried out from human milk which was 40%. The ability of all isolates to produce gelatinase and biofilm has been detected and the results revealed the ability of some isolates to produce gelatinase and biofilm. Ahigh percent of gelatinase production has been detected in L.salivarius isolated from milk sample (50%), while alow percent of gelatinase production was 0% in L.salivarius isolated from saliva. The results of optical density of biofilm produced by L.salivarius isolates from milk which was 100% higher than it in isolates from vaginal and saliva which was 85% and 75% respectively. All isolates shows antibacterial effect against some Gram negative bacteria, the diameter of inhibition as between (2 - 8), the results showed the ability of 4 selective isolates of L.salivarius to produce bacteriocin in which the Rf valve of precipitate bacteriocin was (0.42 - 0.6) compard with leaky bacteriocin hich was (0.57 - 0.8), also leaky bacteriocin as more activity against G - ve bacteria than precipitate bacteriocin. The result of plasmid profile in L.salivarius revealed the presence of megaplasmid in all isolate except one which had one megaplasmid and one small plasmid. Bacterial conjugation between L.salivarius that produce bacteriocin and had megaplasmid which used as adonor cell with E. coli Jm 109 as a recipient cell showed transferring of megaplasmid from donor to recipient cell and the transconjugant cells showed high anti - bacterial effect due to bacteriocin production against G - ve bacteria. The result of amplification of Sal P gene encoding salivracin P by PCR confirmed that all isolate had no Sal P gene in there genetic materials which revealed that these isolates had another type of bacteriocin rather than Salivracin P.

دراسة بكتريولوجية - وراثية البكتريا Acinetobacter baumannii المعزولة من مستشفيات مدينة النجف الاشرف == Bacteriological And Genetic Study Of Acinetobacter Baumannii Isolated From Hospitals In Al-Najaf Al-Ashraf City

Author name: سحر محمد جواد
Supervisor name: احلام كاظم نعيم الياسين
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: English
University location: Najaf
First pages:

فاعلية عسل النحل الطبيعي ضد الزوائف الزنجارية والعنقوديات الذهبية المخمجة للجروح في الجرذان المهقاء == Efficiency of natural bee honey against Pseudomonas aeruginosa and Staphylococcus aureus associated with infected wounds in albino rats

Author name: رغد محمد علي جاسم الدعمي
Supervisor name: جميل جري يوسف الحميداوي
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:

دراسة مناعية لحالات العقم الناتجة عن الاصابات البكتيرية لدى الرجال في محافظة النجف الاشرف == Immunological Study of Infertility Cases in Male which Associated with Bacterial Infection in AL-Najaf AL-Ashraf Governorate

Author name: قيس خضير عبد العباس الحدراوي
Supervisor name: سهام جاسم محسن الكعبي | احسان عيدان عبد الكريم السيمري
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:

استخدام تقنية التفاعل التضاعفي التسلسلي للتحري عن بكتريا Gardnerella vaginalis في حالات الخمج المهبلي البكتيري غير المتخصص == Using of Polymerase Chain Reaction Technique for Detection of Gardnerella vaginalisIsolates In Non-Specific Bacterial vaginosis

Author name: حنان خالد دايخ الظالمي
Supervisor name: احلام كاظم نعيم الياسين
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:

دراسة بكتريولوجية ووراثية للمكورات العنقودية الذهبية المقاومة للمثيسيلين المعزولة من مستشفيات مدينة النجف

Author name: ميعاد كاظم علي الخضيري
Supervisor name: علي محسن المحنة
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:

دراسة التاثيرات السمية لحبوب الحنطة الملوثة بالفطرين Aspergillus flavus وAspergillus niger في الجهاز التناسلي لاناث الجرذ الابيض وامكانية السيطرة عليها في المخزن == Study of the toxic effects of the fungi Aspergillus flavus and Aspergillus niger in the reproductive system of females of Albino rats and the ability to control it in the store

Author name: عذراء حرجان محسن
Supervisor name: سامي عبد الرضا علي الجميلي
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:

دراسة الفعالية التضادية لمستخلصات اوراق نباتي اليوكالبتوس Eucalyptus camaldulensis D. والزعتر Thymus vulgaris L. في جرثومة Staphylococcus aureus خارج جسم ذكور الفئران البيض وداخله == of white mice Eucalyptus camaldulensis D.and Thymus vulgaris L.on Staphylococcus aureus in vitro and in males Study of antibiosis activity of extracts of

Author name: سهير عبد الكريم حبيب الرماحي
Supervisor name: عباس جواد الصراف | ثامر خضير مرزه
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:

دراسة بكتيريولوجية لمسببات تجرثم الدم الهوائية في النجف == BACTERIOLOGICAL STUDY ON EAROBIC CAUSES OF BACTERAEMIA IN NAJAF

Author name: جنان محمد حسين فيروز علي
Supervisor name: محمد صبري عبد الرزاق السعيد
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:

التحري عن حامض الكولانيك في بعض العزلات المرضية لبكتريا Klebsiella pneumoniae == Investigation of Colanic Acid by Some Isolates Klebsiella pneumoniae

Author name: غصون محمد علي وداي العطية
Supervisor name: محمد صبري عبد الرزاق السعيد
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:

امكانية تصنيع مبيد احيائي من الفطر Trichoderma harzianum Rifai لمكافحة مرض تعفن البذور وموت البادرات في الحنطة == Possibility of manufacturing Bio-fungicide from Trichoderma harzianum Rifai fungus to control seed decay and damping-off on Wheat

Author name: صباح لطيف علوان
Supervisor name: سامي عبد الرضا الجميلي
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:

التحري عن بعض انزيمات البيتالاكتاميز في العزلات السريرية لبكتريا الزوائف الزنجارية في مدينة النجف == Investigation of some B-Lactamases in Clinical Isolates of Pseudomonas aeruginosa in Najaf City

Author name: الهام جواد كاظم بلال
Supervisor name: علي محسن المحنة
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:

دراسة بكتريولوجية ومناعية للبايوسين المستخلص من الزائفة الزنجارية المعزولة محليا وتاثيره على الخلايا السرطانية خارج وداخل الجسم الحي == Bacteriological & Immunological Study on Pyocin Extracted from Local Isolate of Pseudomonas aeruginosa and its Effect on Cancer Cell in vivo and in vitro

Author name: ماجدة مالك متعب
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:

دراسة تاثير مستخلصات نباتي الياس L. Myrtus communis والثوم Allium sativum L. في بكتريا Pseudomonas aeruginosa خارج وداخل الجسم الحي == Study of effect of extracts of Myrtus communis L. and Allium sativum L. on Pseudomonas aeruginosa in vitro and in vivo

Author name: نبراس يحيى عبد الله السلامي
Supervisor name: عباس جواد الصراف | ثامر خضير مرزه
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:

دراسة ميكروبيولوجيه لالتهاب عنق الرحم بين النساء في مدينة النجف الاشرف == Microbiological Study of Cervicitis among Women in Najaf AL-Shraf City

Author name: ابتهال اسماعيل عبد الله الشمرتي
Supervisor name: جعفر كاظم نعمة الموسوي
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:
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