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عزل وتشخيص جرثومة اللجيونيلا في ابراج التبريد ودراسة تواجدها في القناة التنفسية للعاملين في الشركة العامة لصناعة الاسمدة الجنوبية في البصرة == Isolation and Diagnosis of Legionella in The Cooling Towers and Study on Their Presence in The Respiratory Tract of The Workers of Southern General Company for Fertilizers Production in Basrah

Author name: جندي علك مهدي البوهلال
Supervisor name: هديل توفيق الحديثي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:

تداخل تاثيرات المواد الحافظة في حماية بكتريا التسمم الغذائي

Author name: محمد جاسم محمد
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
University location: Basrah
Key words:
  • اطروحات الرياضيات - الدوال تحليل

دراسة مسحية للبكتريا المرتبطة بالتهاب الزائدة الدودية وتحديد الحالة النسجية للمصابين في محافظة البصرة == Survey of bacteria associated with appendicitis and determination of Histological patient state in the province of Basrah

Author name: هدى عبد الرحيم مذكور
Supervisor name: محمد عبد الامام احمد | نوري حنون جاسم
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study has been done to determined pathogenic bacteria that associated with appendicitis .This study includes ninety samples of removal appendix taken form patients who cleared diagnosed as appendicitis infection by specialised doctors in general Basrah hospital and Al - Sadir teaching hospital for the period between September - 2013 and June - 2014.Distribution of appendicitis appear in all age groups, 10 - 20 year and 20 - 30 year were the most infected, as well as the infection appear in both gender, but rate of infection in male was relatively higher than female 68(59%), 47(41%) respectively. The ratio of the city's population has overcome the rural population 96(83.5%),19 (16.5%) respectively. The percentage of samples that gave positive culture was 80( 88.9%), while 10 (11.1%) of these samples negative culture. The study reveals 15 different bacterial isolation, the most common bacteria was Escherichia coli 80(44.9%) while other species was appears in less percentage Shigella dysenteria 14(7.9%), Salmonella enterica typhi 10( 6.5 %), Pseudomonas aeruginosa 8( 5.6 %) , Klebseilla Pneumonia 6(4.3%) , Klebseilla oxytoca 3(1.7%), Morganella morganai 7 ( 9.3 %) Neisseria spp. 6 ( 9.5%) , Enterobacter cloaca 4 ( 2.2 %) ,Serreatia spp. 3 )7.1 %(, Citrobacter ferundii 2 (7.7%) Proteus vulgaris 7) 6.5 %( Staphylococcus spp. 14(1.3%), Streptococcus spp. 72(5.1 %) and Bacillus subtilis 8)5.6 %(. Laboratory diagnosis for blood samples included estimated of total WBCs and found that (31%) of patients have natural WBCs values while the other patients have high values.Antibiotic sensitivity test to E.coli isolates towards 20 antibiotics because she is the most common bacteria showed that all isolates were resisted for most antibiotics which used in test especially for β - lactam group, and the isolates of E.coli were multi resistance for antibiotics.Identified minimum inhibitory concentrations MIC for some antibiotics The results showed that E.coli bacterial isolates's 30 isolation have shown resistance to anti AMO as MIC values ranged from 128 - 1024 Mcgm / ml while the MIC for Anti AMP values between 4 - 128 Mcgm / ml, As for antibiotics and Cefixim , Ceftriaxone, Cefotaxime, and it was her MIC values ranging between (1 - 128) Mcgm / ml Plasmid profile of E.coli isolates investigated to study the correlation between plasmid profile and antibiotic resisitant marker and results from agarose gel electrophoresis revealed that all E.coli isolates contain one plasmid band.This study includes the detection of some genes that encode to beta - lactamase enzymes in E.coli which were responsible about multi antibiotic resistanc and these genes loaded on plasmid DNA for ten isolaes and found that 5(50%) from isolates have blaTEM gene and 5 )40%( have blaCTX gene and 1(10%) have blaSHV gene. This study also considered note the general appearance of appendix samples , some of them are enlarged and surrounded by vesicles, some with fibrous walls and ulcerated with mixed colors, then examined the histological changes it, the study showed changes in histological structure of the excess was extensively congestion of blood vessels, veins in serosa and subserosal layers and increased the amount of diffuse lymphoid tissue in the layers of the appendix walls.

دراسة وبائية وجزيئية للجرثومة Escherichia coli O157 : H7 المعزولة من عينات سريرية وبيئية في محافظة البصرة - العراق == Epidemiological and molecular study of the bacterium Escherichia coli O157 : H7 isolated from clinical and environmental samples Basra Governorate / Iraq

Author name: عباس ضرب شعبان
Supervisor name: ميثم ايوب عبد القادر الحمداني
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: A total of 464 clinical and environmental samples attempt to isolate Escherechia coli O157 : H7 serotype were collected, of which 183 stool samples from diarrheal children at Al - Qurna hospital and Al - Medaina Hospital of children during the period between October 2013 - August 2014. Environmental samples (281) : 121 samples of drinking ( tap) water , 80 samples of river water , and 80 samples of sewage water (collected from different regions of Basra Governorate) . All samples was tested and detected the presence of Escherichia coli serotype O157 : H7 using chromogen agar and ( CT - SMA ) as selective media and using direct immunological testing for direct identification of the pathogen (Certeste) for clinical samples.The study revealed the isolation of 56 isolates of E.coli O157 : H7 at a rate of (12.06%) : 16 clinical isolates at a rate of (8.7%), 40 environmental isolates at a rate of (14.23) : 9 isolates from drinking water at a rate of (7.4%), 18 isolation from sewage at a rate of (22.5), and 13 river isolates at a rate of (16.25).Antibiotic susceptibility testing toward 20 antibiotic of E.coli O157 : H7 isolates showed that all isolates from all sources resistant to the antibiotics Ampicillin, Amoxacilllin, , Rifampin and Piperacillin while showed high sensitivity to antibiotics amikacin, Kanamcin, ciprofloxacin and norfloxacin.The clinical isolates of E.coli O157 : H7 showed multiple high antibiotic resistance index Muliple Antibiotics Resistance (MAR index) ranged between (0.25 - 0.8), Where two isolates showed a resistance to sixteen antibiotics, Two isolates showed resistance to twelve antibiotics, Two isolates showed a resistance to eleven antibiotics, one isolate showed a resistance to ten antibiotics,Three isolates showed a resistant to nine antibiotics and two isolates showed aresistance to eight antibiotics, One isolate showed a resistant to six antibiotics and two isolates showed resistance to five antibiotics.The environmental isolates of E.coli O157 : H7 showed less resistance ranged between (0.25 - 0.75) for samples of sewage and (0.35 - 0.50)rivers samples , and (0.30 - 0.55)tap water samples . As shown, one isolate resistant to fifteen antibiotics, one isolate showed a resistance to thirteen antibiotics and four isolates showed a resistance to ten antibiotics from sewage. As for rivers Tow isolates showed a resistance to ten antibiotics, three isolates showed a resistant to nine antibiotics while a one isolate of tap water showed a resistance to eleven antibiotics, three isolates showed a resistance to ten antibiotics and an one isolates showed a resistance to nine antibiotics and two isolates resistant to eight antibiotics.Investigating about the content of plasmid in isolates E.coli O157 : H7 to study the relationship between the plasmid content as an indicator of antibiotic resistance, as the results showed that most of the isolates E.coli O157 : H7 four clinical isolates containing the plasmid including one molecular weight > 10kbp with a Patterns of multiple different resistance,also two isolates contain three plasmids of a molecular weight > 10kb. And one isolate containing four plasmids with molecular weights ranging from (4 - 10Kb) and one isolate containing five plasmids resistant to sixteen antibiotics with molecule weights ranged between >(2 - 10Kb).The isolates E.coli O157 : H7 isolated from the environment , sixteen isolates showed that they contain plasmids of molecular weights> 10kb , one isolate contains two plasmids of molecular weights ranging between (4 - 10Kb), five isolates containing three plasmids of molecular weights ranging from (2.9 - 10) Kb and two isolates containing four plasmids of molecular weights ranging between( 2 - 10 Kb

دراسة فسلجية لبعض انواع من جنس Bacillus المقاومة للمعادن الثقيلة وتحسين قابليتها الاصحاحية == Physiological Study Of Some Bacillus Spp. Resistant To Heavy Metals And Improving Their Ability Of Bioremediation

Author name: ایمان عبعوب مخیفي
Supervisor name: امین عبد الجبار السلمي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: English
University location: Basrah
First pages:

عزل وتشخيص جرثومة Helicobacter pylori من مياه الصرف الصحي في محافظة البصرة باستعمال الطريقة الزرعية وتقنية ال CR ( 16S rRNA ) == Isolation And Identification Of Helicobacter Pylori From Sewage In Basrah Province By Culture Method And PCR (16S Rrna)

Author name: الاء يعقوب الربيعي
Supervisor name: امين عبد الجبار السلمي | اسعد محمد رضا الطائي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: English
University location: Basrah
First pages: