مكان الجامعة: البصرة

الصفحات الاولى:

المستخلص: Twelve isolates of Azotobacter as free nitrogen fixing bacteria were isolated from rhizosphere of different plants and soils in (Thi - Qar and Basrah province) ,two strains were brought from (Thi - Qar university - College of Sciences) and one strain was brought from Ministry of Agriculture ( imported from Italy), all strains were diagnosed by studying biochemical , microscopical and morphological characteristcs .strains (A1,A21and AB) gave maximam efficiency of nitrogen fixation then were used for inoculanting seeds of corn.Agricultural experiment was carried out during spring season 2014 in research station of College of Agriculture - Basrah University in loam soil using CRD design with three replicates to study response of corn for inoculation with Azotobacter intergraded with organic fertilizer (0, 30) ton hectar - 1 and nitrogen fertilizer levels(0,80,120and160) kg N hectar - 1of which represented of (0,50,75,100)% of recommended completed of nitrogen fertilizer(160)kg N hectar - 1.After tow months of growing ,plants were scythed .then dried and estimated : heigh plant, length of root , dry weigh for plant and root, (N,P,K) uptake in plant and root , number of Azotobacter, total residual nitrogen in soil polts.Results obtained from this study : - 1 - A1 locally isolate showed superiority over the rest used isolates in height plants , length root,dry weight of plant and root, (N) uptake in vegetative part in corn and soil total nitrogen residual percentage 18.3%,56.1%,13.6%,64.4%, 56.9% respectively compared with control.2 - Use treatment(A1N2M1) lead to increase height plants , length root,dry weight of plant and root, (N) uptake in vegetative part in corn, number of Azotobacter and soil total nitrogen residual 3 - A21 strain showed superiority over the rest used strains in phosphorus and potash uptake of vegetative part percentage 62.6% and 61.2% respectively.4 - Treatment(A21N2M1) excelled significantlly in phosphorus and potash uptake of vegetative and root of plant .5 - Inculation whith three isolates and adding organic matter (singular or mixed) lead to significant increase all characteristcs studied.

تاثير الباكلوبيوترازول والكازين في تكوين الاجنة الخضرية وانباتها لنخلة التمر Phoenix dactylifara L صنف نيرسي

اسم المؤلف: زياد طارق صافي عبد العلي

الموضوع العام: علوم الزراعة

السنة: 2014

الموضوع الدقيق: النبات

الدرجة: دكتوراه

الجامعة: جامعة البصرة - كلية الزراعة - قسم علوم التربة والموارد المائية

اللغة: العربية

مكان الجامعة: البصرة

الصفحات الاولى:

المستخلص: Present study was conducted in the Plant Tissue Culture Laboratory, Agriculture Collage, Basrah University, Basrah , Iraq, in the period 2012 - 2014. The objective of this study was to evaluate the effect of different concentrations of growth retardant (paclobutrazol) and casein hydrolysate on somatic embryogenesis formation and germination in date palm (Phoenix dactylifera L.) cultured in vitro. The shoot tip explants were excised from offshoots that approximately 3 - 4 years old. Shoot tip explants then cut it in four segments and each segment cultured on Murashige and Skoog (MS) basal medium containing different concentrations of paclobutrazoal (0, 0.3, 0.5, 0.7, 0.9 and 1.0 mg/L) or casein hydrolysate (0, 200, 400, 600, 800 and 1000 mg/L).Results obtained in this study can be summarized to : 1 - Study showed that the MS medium containing pacloputrazol (1.0 mg/L) or casein hydrolysate (1000 mg/L) increased in fresh and dry weight of primary callus and embryogenic callus comparing to control treatment.2 - Primary and embryogenic callus cultured on MS medium fortified with 1.0 mg/L paclobutrazol for a long period showed superiority in fresh and dry weight compared with other treatments and control.3 - The period need it for somatic embryo formation was decreased to (17 and 20.33 days) when the embryogenic callus with 0.5 and 0.7 mg/L of paclobutrazol respectively. Results also revealed that the numbers of white and big spherical somatic embryos were appeared when high concentrations of paclobutrazol 1mg/L and casein hydrolysate (1000 mg/L) have been used.4 - The rate of somatic embryos length were increased when the concentration of casein hydrolysate increased to 1000 mg/L. In contrast, length rate of somatic embryos were decreased when treated with paclobutrazol.5 - High fresh and dry weight of somatic embryo were recorded on MS medium containing (0.9 and 1 mg/L) of paclobutrazol.6 - The high germination rate of somatic embryos was obtained on MS medium containing 0.3 and 0.5 mg/L paclobutrazol or MS medium enriched with 800 and 1000 mg/L in short time in comparing with other treatments.7 - Among the different concentrations of paclobutrazol tested, 0.3 and 0.5 mg/L were the most effective concentrations for increase the length of leaf and primary roots, but the 0.7mg/L and 0.9 led to increase root numbers only, whereas the high concentration of paclobutrazol 1.0mg/L increased the length of leaf and decreased the rate of roots. In contrast the high concentrations of casein hydrolysate (800 and 1000 mg/L) affected positively on leaf and root length.8 - Embryogenic callus tissue subjected to 1.0 mg/L paclobutrazol, containing high carbohydrate, whereas, embryogenic callus treated with 0.5 and 0.7 of paclobutrazol containing high protein. In contrast, embryogenic callus tissue treated with different concentrations of casein hydrolysate containing high carbohydrate and protein.9 - Histological study revealed that the high concentration of paclobutrazol reduced the size of cells in compare with control treatment.