Author name:
عقيل كريم حسن الطفيلي
Supervisor name:
جمال احمد عباس السيد سلمان | صباح نعمة كامل الثامر
University location:
Najaf
Abstract:
The experiment was designed by using a ompletly randomized split plot design in three replicates during the two growing seasons (2012 - 2013) and (2014 - 2015) in an Alsahla area/province of Najaf to study two cultivars of leek response (local and Syrian) Allium ampeloprasum L. to four fertilization treatments (control, the nitrogen fertilization, Azotobacter bacteria and enriched bio - fertilizer EM1), and spraying of Putrescine, in three concentrations (0, 75 and 150 mg.l - 1) in addition to their interaction on each other on the growth traits and plant content of active substances.The experiment composed of 24 treatments, Averages were compared using the least significant difference at the level (P<0.05), The results showed the following : : egetative growth charactersV - 1A significant superiority of the local cultivar for both seasons as compared with Syrian cultivara. The Syrian cultivar showed a significant increase in the diameter of bulbs for both seasons as well as in the fresh weight of bulbs and number of roots for the first season and the plant height for the second season.Fertilization factors significantly effected all of vegetative growth traits for both seasons in a nitrogen fertilization for both seasons a significant increase in the number of roots and fresh roots weight and for the first season, in the number of leaves/plant - 1, leaves dry weight. While in the second season, it showed a significant increase in bulb diameter. Treatment with Azotobacter for both seasons showed a significant increase in the fresh weight of leaves, the dry weight of bulbs and dry weight of roots, and in the first season, in plant hight and diameter of bulbs, but in the second season the same treatment had significantlyBaffected in number of leaves, the dry weight of leaves and the fresh weight of bulbs. The bio - fertilizer EM1 showed a significant increase in the fresh weight of bulbs in the first season, where as in the second season, EM1 fertilizer showed a significant increase in plant height. The interactions between all factors significantly affected in all traits.Spraying of Putrescine had a significant effect on vegetative growth. Spraying with Putrescine (150mg/l - 1) for both seasons showed a significant increase. There is no significant differences between all Putrescine concentrations in roots number during the second season. The interactions between all factors significantly affected in all comparisons in both seasons.2. The chemical content of leaves and bulbs of leek : The "Syrian" cultivar showed superiority over the "local" cultivar for both seasons in leaves content of carbohydrates and the ratio of total solids dissolved materials reaching (30.08 and 21.00%) and (6.33 and 5.20%) for both seasons respectively, as compared with the "local" cultivar (29.70 and 18.99%) and (5.64 and 4.53%) respectively, in the first season "Syrian" cultivar showed superiority over in the percentage of protein in leaves and bulbs, leaves and bulbs content of nitrogen, bulbs content of phosphorus and sulfur, leaves and bulbs of potassium and the content of total chlorophyll of leaves and content of bulbs of carbohydrates and leaves content of sulfur for the second season, but the "local" cultivar showed superiority in content of carbohydrates of bulbs and phosphorus, sulfur of leaves content and leaves content of total chlorophyll for second season. In addition to other important results.Nitrogen fertilization showed superiority on other fertilization factors for both seasons in leaves content of carbohydrates (in comparison with the control treatment), protein and nitrogen (in comparison with EM1 treatment), and a rateCincrease reaching (14.04 and 35.43%), (38.92 and 13.15%) and (38.16 and 13.11%) respectively, and in the first season, the nitrogen fertilization showed a significant increase in content bulbs of protein, nitrogen and potassium, and the total chlorophyll content in leaves, and in the second season in the content of bulbs in carbohydrates and content of potassium of leaves, while treatment with Azotobacter showed a significant increase for two seasons in bulbs and leaves of phosphorus (in comparison with control treatment), and bulbs content of sulfur (compared with control treatment for first season and EM1 treatment for the second season) and a rate increase reaching (31.77 and 17.62%), (18.08 and 34.53%) and (19.20 and 21.60%) respectively, and in the first season Azotobacter treatment showed a significant increase in the percentage of total solids dissolved materials in leaves, leaves content of potassium and sulfur and bulbs content of carbohydrates, while in the second season, treatment with Azotobacter showed a significant increase of bulbs content of protein, nitrogen, and potassium, but treatment of bio - fertilization EM1 showed a significant increase in leaves content of the percentage of total solids dissolved materials, and total chlorophyll and sulfur in the second season. The interactions between all factors significantly affected in all comparisons in both seasons.Putrescine (150mg/l - 1) showed superiority significant increase for two season over other concentration, as compared with control treatment in the content of leaves and bulbs from carbohydrates, and a rate increase reaching (23.39 and 24.12%) and (34.69 and 8.23%) respectively. The concentration of putrescine (75mg/l - 1) gave high value and for two seasons in leaves content of the total chlorophyll and a rate increase reaching (8.84 and 20.78%), and the sulfur (compared with 150mg/l - 1 for first season and control treatment for second season, and a rate increase reaching (21.38 and 8.92%) respectively, and no significant difference between putrescine concentrations in leaves content of the total solid dissolved materials and potassium for the second season.D3. the active substances content of leaves and bulbs of leek : "local" cultivar showed superiority for both seasons in leaves and bulbs content of Allicin, Quercetin, kaempferol and total phenolics, and the content of leaves of the total flavonoids, and the content of the total flavonoids in bulbs during the second season, while no significant difference between two cultivars in bulbs content of the total flavonoids in the first season.The treatment of Azotobacter showed a significant superiority for both seasons in the content of leaves and bulbs of Allicin, Quercetin, kaempferol, nitrogen fertilization also significant superiority, for both seasons in bulbs content of the total phenolics and the content of leaves of the total flavonoids and the content of leaves of the total phenolics in the first season, and the bio - EM1 significant superiority on bulbs content for both seasons in the total flavonoid content and the content of leaves in the total phenolics in the second season.The superiority of Putrescine at (150mg/l - 1) concentration showed significant increase on other concentrations and for both seasons in leaves and bulbs content of Allicin, Quercetin, kaempferol and total flavonoids, and content of leaves of the total phenolics in the first season, but in the second season, there are no significant differences between the two cultivars. but the content of bulbs of the total phenolics the (150mg/l - 1) concentration gave higher results in the first season, but the second season (75mg/l - 1) concentration superiority and gave higher results.
