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عزل وتشخيص بكتريا Enterococcus faecalis من مصادر مرضية مختلفة ودراسة امراضيتها في الفئران المختبرية Isolation And Diagnosis of Enterococcus Faecalis From Different Clinical Sources And Study of The Pathogenicity in Experimental Mice

اسم المؤلف: عباس ياسين حسن
اسم المشرف: وعد محمود رؤوف عباس عبود فرحان
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية بهدف عزل وتشخيص المكورات المعوية البرازية Enterococcus faecalis من مصادر سريرية مختلفة، وتقييم التغيرات المظهرية والافات المرضية في بعض اعضاء الفئران البيض السويسرية المصابة تجريبيا لذا تضمنت هذه الدراسة محورين رئيسين هما : الجانب ال | The present study was conducted to isolate and diagnose an Enterococcus faecalis which are isolated from different clinical sources, and evaluate the appearance changes and pathological lesions in some Swiss white mice organs which are infected experimentally, so this study deals with two main aspects : The bacteriological type : The results obtained may be summarized as follows : - The total number of (310) clinical samples were collected from Baquba Educational Hospital and Al - Batol Hospital for Maternity and Children in Baquba city from the beginning of March to the end of September (2009) with various ages and from both sexes, including (82) urine samples from patients with urinary tract infections, (46) vaginal swab from infection of female reproduction system, (45) surgical wound swabs, (38) stool sample from patients with abdominal pains and diarrhea, (32) throat swabs from tonsillitis patients, (25) middle ear swabs, (22) burn swabs, and (20) blood sample from patients with bacteremia and prosthetic valvular heart diseases. The isolates diagnose depended on the cultural and microscobial characteristics and biochemical tests, serological diagnosis according to Lancefield method and assertion diagnosis according to (API - 20 Strep) was done to find (32) isolates of Enterococcus : (28) isolates of E. faecalis (87.5%), (3) isolates of E. faecium (9.4%) and one isolate of E.gallinarum (3.1%). - The sensitivity of E. faecalis was tested against (23) different antibiotics. The isolates showed resistance of about (100%) against Aztreonam, Amikacin, Co - Trimoxazole, Cephalexin and Cefotaxime, while the isolates were sensitive about (100%) against Amoxicillin, Nitrofurantoin, Imipenem and Ampicillin, while they showed a various resistance to another antibiotics involved in this study. - The ability of the isolates for producing the Beta - lactamase enzymes was tested by the standard rapid iodometric method. The results showed the disability of these isolates for producing the Beta - lactamase enzymes. - The production ability of E. faecalis for some virulence factors was evaluated. The results showed the ability of these isolates of adherence to the uroepithelial cells of human's urinary system were (93%), and (82.1%) were bacteriocin producer, and (28.6%) were hemolysin producer, and (43%) were gelatinase producer, and (10.7%) were lipase producer, and (35.7%) were capsule producer, and (14.3%) were cytolysin producer, while none of the isolates under the study showed the ability of haemagglutination.Histopathology type : (4.6 x 106) cell / ml concentration of the bacterial suspension of E. faecalis was used orally on mice and histopathological changes were examined after (3, 5, 7, 14) days of infection, which revealed the ability of bacteria for colonization and causing pathological lesions and many histological changes in the liver, kidney, small intestine and hearts of these animals. The damage in the animal tissues of the second group (treated with Streptomycin and EFU10) was more than that in the third group (treated with Streptomycin and EFS14), and fourth group (treated with EFU10 only). Generaly, the most important histopathological changes were the necrosis in some areas, and infiltration of inflammatory cells in infected tissues. These pathological lesions cause damage and decompose of tissues with distortion of their morphologies.

دراسة وبائية تشخيصية لداء اللشمانيا مع تحديد انماط الطفيل المسبب بواسطة تقنية PCR وتمييز الانواع الناقلة من ذبابة الرمل sand fly في محافظة ديالى Epidemio Diagnostic Study for Leishmaniasis With Determination of Parasite Strain’s By PCR Technique And Morphologically Differmates The Species of Sand Fly Vector in Diyala Governorate

اسم المؤلف: جابر عودة كاظم
اسم المشرف: توفيق ابراهيم محمد الالوسي عبد الله حسين عبد الله الجبوري
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية تسليط الضوءعلى انتشار داء اللشمانيا الجلدية والاحشائية المتسبب من قبل الطفيلي Leishmania spp في مناطق مختلفة في محافظة ديالى ودراسة الظروف البيئية المساهمة في انتشار المرض ومقارنة الفحوصات السريرية والمختبرية والمصلية المستخدمة في تش | The aims of present study to illuminate a light on visceral and cutaneous leishminiasis disease caused by leishminia Spp parasite as registered in Diyal Governorate, and study the environment condition which contributed in diffusion the disease and comparison the tests of clinical, Laboratory and serological tests which used in diagnosing with PCR technique test, the study included many steps. 1. The epidemiologically which is main to determine the rates of infection as registered in some district of Diyala province in according to the age, sex, and the month of the year so according to the environmental conditions : - 7200 samples of blood collected from many different ages persons from different districts in Diyala. (3009 males, 4011 females) are examined. The study shows a positive cases of 607 distributed among the districts including (Jalawlaa, Bani Saa'd, Baladruz, Baquba, Alodeim amd DeliAbbas) these register a highest percentage of infection in Jalawlaa sub - district about 12.8% and less percentage of infection are in Baquba about 1.3% whiel is register highest percentage of in infection were (>1 - 5) years about 13.2% and lower percentage infection (<5 - 10) years wuth rate 2.4% so the groups of ages (25 - 30) years didn't appear any infections. according to the gender the males are high from the females whom effected with this disease the number of males (432) case with rate 71, 1%. The number of infected females about 175 cases with rate 28.8%.while in the month of years were the male infection are a highest infection in January - 2012, but did not register any infection in (June and July). The environmental condition infective in prevalence the disease between the people whom resident the houses which breed the animals inside, the number of infections are 245 cases from 607 positive cases with rate 40.3%. This was the low rate 4.9% from the infected people because of rats in their hoses. which didn't kill them. 2. The comparison of diagnostic methods of clinical test, laboratory test and serological test, with the molecular methods from the direction of sensitivity and specificity, The studies results appeared the importance of clinical test subtle in case of a similar cases depend on the clinical symptoms there are a highest cases from clinical test that the children suffering from liver magle their number are 176 cases the rate 80.36%, but the lowest cases of clinical test are 59.19% whom suffered from a skin pallor and black. The laboratory tests appeared the blood picture through testing the 350cases whom suspicion in their infection with leishmaniasis, the number of whom suffering from the lower of WBC 73 with rate 34.2%, while the low cases are 6 with rate 2.82% from whom suffering from hipper ESR, but the serological test was IFAT, Dipsrtick, spotkala - azor, ELISA, KA tex LATEX. Diagnostic infection leishmaniasis a best in diagnostic disease is dipstick test it's sensitivity are 95%, but it's specificity 100% but the lowest is the test of LATEX, it's sensitivity 75% and specificity 80%.3. The Molecular methods : PCR technique test clear recently the new highest sensitivity in diagnosis when the number of the parasite are a little in the blood in the infections peoples whom choose in random way. when testing 100 samples of DNA from the infection with cutaneous and visceral leishmaniasis, the number of infected male are 73, But the number of infected females are 27 with leishmaniasis. and studying the relation between PCR diagnosis according to the sex, age, district and environment condition, so as the recognize between the species of the parasite from the sequence Nitrogen basics with number in each species, (the species are L.donovani, L.infantum, L.tropica, L.major) so recognize the vector insect sand fly Morphology by key classification (ph.alexandri, ph.papatasi Ph.sergentasi, and sqauanlpleuris).

دراسة بيئية لمجتمع الطحالب وبعض الملوثات البيئية في نهر الحسينية - كربلاء المقدسة / العراق Ecological Study For Algal Community And Some Pollutants In Al - Husseinia River Holy Karbala - Iraq

اسم المؤلف: سارا حمود عبد الامير الاسدي
اسم المشرف: فكرت مجيد حسن جاسم محمد سلمان
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الاحياء المائية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:
المستخلص: اجريت دراســة بيئية شاملة على مياه نهر الحسينية في الفترة الممتدة مـن تشــرين الاول 2012 ولغاية ايلول 2013، تم فيها دراسة الخصائص الفيزيائية والكيميائية لمياه النهر بالاضافة الى اجراء دراسة كمية ونوعية لمجتمع الطحالب الهائمة والملتصقة المتواجدة في المناطق | Acomprehensive ecological study was conducted on Al - Husseinia River during the period from October 2012 to Septemper 2013. This study includes measuring some chemical and physical properties of water and conduct quantitive and qualitative study of the Algal community which includes the phytoplankton and the periphyton algae , in addition to measuring some of pollutants types such as Heavy metals and Polycyclic Aromatic Hydrocarbons (PAHs) the water of the river and its sediments and some kinds of aquatic plants such as Ceratophyllum demersum, Hydrilla verticillta, Potamogeton pectinatus and Phragmites australis. The results of the present study showed temporal and spatial variations in the physical - chemical properties of water. The water temperature ranged between (12.7 - 31.3) °C which accompanied the variations of air temperature that ranged between (8.5 - 45.3) °C. The river was tending to the alkalinity and the PH was ranged between 7.5 - 8.8 were dominated by bicarbonate ions, the electrical conductivity and the Total Dissolved Solids and The Total suspended solids ranged between (840 - 1416.67) ?s/cm and (413.33 - 700) mg / l and (6.33 - 55.07) mg / l respectively. The river was oligosaline with a salinity ranged between (0.54 - 0.91) PSU. The water current velocity and light penetration recorded fluctuated during the period of the study, their values ranged between (11.68 - 37.77) cm / Sec and (33.67 - 148.67) cm, respectively. The Dissolved oxygen and biological oxygen demand increased during the cold months and decreased during the warm months where the values ranged between (4.97 - 12.63) mg / l and (0.4 - 4.48) mg / l respectively. The river was very hard, the total hardness ranged between (240 - 516.67) mg / l, and the Total alkalinity ranged between (106.33 - 162) mg / l. Calcium ions and Magnesium ions ranged between (40.08 - 162.32) mg / l and (13.67 - 64.73) mg / l. The values of sulfates ranged between (58.94 - 131.37) mg / l ; their values show an increase at the study sites which were under highly human activities, but they did not exceed the permissible levels of the drinking water quality. The nutrients such as silica and total nitrogen and total phosphorous showed variations during the study and their values ranged between (1.63 - 6.26) mg/l and (1.4 - 3.5) mg / l and (1.33 - 17.29) ?g/l, respectively. A total of 303 algal taxa of phytoplankton and periphyton was identified during the study belonged to six classes of Algae; they include Bacillariophyceae (196 taxa) , Chlorophyceae (59 taxa) , Cyanophyceae (33 taxa) , Euglenophyceae (6 taxa) , Dinophyceae (6 taxa) and Cryptophyceae (3 taxa). The Bacillariophyceae were a dominated class in the phytoplankton community, they constitute 62.4 % of the total number of species followed by Chlorophyceae by 20.66 % and Cyanophyceae by 11. 6 % respectively. The Dinophyceae and Euglenophyceae constitute 2.07 %, while the cryptophyceae constitute the lower percentage which amounted to 1.2 % of the total species. Some identified algae like as Nitzschia, Navicula, Cymbella, Fragilaria, Scenedesmus and Oscillatoria were dominated by their species numbers in the phytoplankton community , and the total numbers of the phytoplankton cells ranged between (446.8 × 10 3 - 9177 × 10 3) cell / l. In October recorded the highest number of the cells, while in March recorded the lower numbers of the algal cells during the study. A total of 203 taxa was characterized in the epipelic algal community dominated by Bacillariophyceae by 79. 3 %, followed by Cyanophyceae by 10.84 % and Chlorophyceae by 6.9 % respectively, while the Euglenophyceae and Dinophyceae constitutes the lower percentage formed 1.48 % of the total species. The total number of epipelic algal cells ranged between (9924.5 to 10.02 × 104) cells / cm2. Winter season recorded the highest average of the total number of cells, while Spring season recorded the lowest numbers of cells. Also, some genera such as Nitzschia, Navicula, Cymbella, Fragilaria and Oscillaroria were dominated in the study period. During this study some taxa of epiphytic algae have also identified on four types of aquatic plants, which included C.demersum , H.verticillata, P.pectinatus and P.australis, the numbers of algal taxa on each there were 124, 116, 77 and 114 respectively. The results showed dominated of Bacillariophyceae followed by Chlorophyceae and Cyanophyceae and other classes respectively. Some algal genera are dominated by their species of the studied plants such as Cymbella, Navicula, Nitzschia, Fragilaria and Gomphonema. In Autumn the highest numbers of epiphytic cells were recorded, where the total numbers of cells in these plants are 74.24 × 104 cells/g, 38.46 × 104 cells/g, 19.22 × 104 cells/g and 38.88 × 104 cells/g respectively. While the lower number of cells recorded during Summer in each of C.demersum by 8.07 × 104 cells/g and H.verticillata by 8.034× 104 cells/g and P. pectinatus by 3.74 × 104 cells/g, whereas the P. Australis recorded the lowest number of cells during Spring which was 22.51 × 104 cells/g. C.demersum contained the highest numbers of algal cells compared to other plants. The distribution and concentrations of some Heavy metals such as Cd, Cu, Pb, Fe and Zn are studied in each of the water (dissolved and particulate fractions), and sediments (exchangeable and residual fractions) and four aquatic plants. The average of these heavy metals concentrations in the dissolved phase of the water were (1.4, 1.15, 3.74, 5.46 and 7.66) ?g / l, respectively. While their concentrations in the particulate phase were (3.67, 110.23, 63.97, 732.98 and 167.2) ?g/g respectively. In the sediments the concentrations of these heavy metals in the exchangeable phase were (2.53, 38, 43.45, 169 and 42.16) ?g/g, respectively, whereas their concentrations in the residual phase were (2.54, 32.82, 50.33, 1452.75 and 53) ?g/g, respectively. The concentrations of the Cd, Cu, Pb, Fe, Zn in the aquatic plants are also measured during this study and the average of theirs in C.demersum were (11.09, 14.16, 48.58, 2202 and 65.42) ?g/g, respectively. While their concentrations in the H. verticillate were (10.17, 12.99, 43.58, 2921.98 and 60.45) ?g/g, respectively. In P. pectinatus were (11.58, 13.52, 50.06, 2315.47 and 77.88) ?g/g, respectively. Whereas their concentrations in P.australis were (9.29, 11.83, 39.84, 1845.21 and 52.76) ?g/g, respectively. The distribution of sixteen Polycyclic Aromatic Hydrocarbons is also determined in Al - Husseinia River during this study in each of water, sediments and aquatic plants. These organic compounds include Naphthalene، Acenaphthylene، Acenaphthene، Fluorene، Phenanthrene، Anthracene، Fluoranthene، Pyrene، Benzo(a)Anthracene، Chrysene، Benzo(b)Fluoranthene، Benzo(k)Fluoranthene، Benzo(a)Pyrene، Dibenzo(a, h)Anthracene, Benzo(ghi)perylene وIndo(1, 2, 3 - cd)Pyrene. Their concentrations in the water of river ranged between (0.24 - 58.72) ng/l recorded for each of Benzo(a)Pyrene and Benzo(ghi)perylene respectively, the total concentration of PAHs in the water was 152 ng/L, the results also showed that the source of water pollution of these compounds was pyrogenic and petrogenic origin. In the sediments the concentration of these compounds ranged between (0.36 - 119.06) ?g/g. For each of Naphthalene and Benzo(ghi)perylene, respectively. The total concentration of PAHs in the sediment was 257.71 ?g/gm., The results also showed that the source of sediment pollution with these compounds was also pyrogenic and petrogenic origin as the water. In aquatic plants, the concentration of these compounds in C.demersum and H.verticillata ranged between (0.12 - 152.3) ?g/g and (0.3 - 129.8) ?g/g for Fluoranthene and Benzo(ghi)perylene to each plants respectively , while their concentration in P.pectinatus ranged between (0.25 - 99.36) ?g/g for each Benzo(b)Fluoranthene and Benzo(ghi)perylene respectively, in P.australis PAHs concentration ranged between (0.37 - 134.85) ?g/g for Chrysene and Benzo(ghi)perylene, respectively. C.demersum recorded the highest total concentration of PAHs compared to other plants, and the results showed that the source of pollution in these plants was Pyrogenic origin. They also revealed that Benzo(ghi)perylene recorded the highest concentration during this study compared with other compounds.

دور الكاينيتين والسماد المركب (NPKZn) في تحمل نبات الفلفل Capsicum annuum L.)) للاجهاد الملحي Role of The Kinetin And NPKZn In Salt Stress Tolerance of Sweet Pepper (Capsicum Annuum L.)

اسم المؤلف: سعاد عبد سيد الجلالي
اسم المشرف: عباس جاسم حسين الساعدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت تجربة اصص في الحديقة النباتية التابعة لقسم علوم الحياة، كلية التربية للعلوم الصرفة - ابن الهيثم، جامعة بغداد في الموسم الربيعي عام 2014 هدف الدراسة كان دراسة تاثير تركيزين من كلوريد الصوديوم هي 50) و100) مليمول.لتر - 1 فضلا عن معاملة السيطرة واربعة | The aim of the pots experiment which was conducted in the Botanical Garden of Biology Department in the Faculty of Education /Ibn - Al - Haytham / University of Baghdad, during the spring season of 2014 was the influence of different concentrations of Sodium chloride (50, 100) mM.L - 1 in addition to the control treatment and and Four concentrations of kinetin (25, 50, 75, 100) ppm in addition to the control treatment.It is also aiming to study the effect of application and non application the level 160 Kg.H - 1of NPKZn fertilizer, and their interactions in some morphological characteristics root length, root size, plant height, the internode length, stem diameter, Leaf area, dry weight for root and shoot and the absolute growth rate for shoot.and some physiological characteristics Elements content and protein ratio in root, Elements content, protein ratio, total chlorophyll content, carbohydrates percentage and cytokinine concentration in shoot. Biochemical characteristics (enzyme activity of the enzymes) (SOD, POD and CAT) and some antioxidants that nonenzymatic : (the proline and vitamin C concentration) in shoot. Malondialdehyde (MDA) content in shoot, and some Floral morphological, physiological and biochemical characteristics of fruits (Number of branches, The total number of flowers, Number of blooming flowers, ratio of aborted Flowers, Number of knotted flowers, the number and size of fruits, their wet weight , nitrogen and calcium concentration, protein percentage and vitamin C concentration in fruits of pepper plant California weleander variety.The experiment was designed according to Randomized Completely Block Design (RCBD)with three facters kinetin, manure addition and Sodium chloride,) consisting 90 pots. Seedling of pepper plant was planted on 4/2/2014 acompanied by making all agricultural operations of irrigation and the removal of the jungles. results were obtained can be summarized as follows : 1 - The results showed that increasing the concentration of sodium chloride from zero to 100 mM.L - 1 Led to a decline in morphological, physiological and floral characteristics, with and significant increases in the content average of sodium, chloride, The proportion of flowers aborted and biochemical characteristics. The rate quality effectiveness for enzyme SOD increased from 7.24 to 34.66, POD from 8.10 to 26.35 and CAT from 4.91to 39.57 (unite mg protein - 1) , and proline acid content from 23.05 to 40.30 (mg.L - 1) and vitamin C concentration from 2.67 to 3.68 % and Malondialdihyde (MDA) from 1.84 to 3.86 (µM.gm F.W. - 1)2 - As concerns kinetine treatment effect, the results showed a significant increase in the average of morphological, physiological and floral characteristics, and significant decreases in the average of sodium, chloride contents and biochemical characteristics, in the cased increasing in the concentration of kinetine from 0 to 100 ppm caused decreases in the average of quality effectiveness for enzyme SOD from 25.79 to 21.91, POD from 21.83 to 17.20 and CAT from 27.88 to 21.73 (unit mg protein - 1) , and proline acid content from 34.27 to 31.68 (mg.L - 1), Malondialdihyde (MDA) from 3.15 to 2.85 (µM.gm F.W. - 1) and vitamin C concentration from 3.42 to 3.23 %.3 - application the level 160 Kg.H - 1of NPKZn Manure caused a significant increases in all averages morphological, physiological and floral characteristics, and significant decreases in the average of sodium and chloride contents and biochemical characteristics, fertilizing with the level 160 Kg.H - 1of NPKZn fertilizer caused decreases in the average of quality effectiveness for enzyme SOD from 22.03 to 19.88, POD from 17.70 to 14.53 and CAT from 23.34 to 17.34 (unite mg protein - 1) , and proline acid content from 32.84 to 28.93 (mg.L - 1), Malondialdihyde (MDA) from 2.97 to 2. 75 (µM.gm F.W. - 1) and vitamin C concentration from 3.29 to 3.03 %.4 - Results of the bilateral interpenetration between the Fertilization by fertilizer NPKZn and Sodium chloride concentrations, showed that the fertilizations has a positive and effective influence in reducing the negative effects of sodium chloride in all characteristics studied. 5 - The results showed the positive role of kinetine in decreasing the negative effects of sodium chloride Through bilateral overlap between factors. Especially when the concentration of 75 ppm of kinetine and concentration 100 of sodium chloride In all morphological, physiological and biochemical floral characteristics of plant.6 - The results indicated that the Interference between the fertilizer NPKZn and kinetine has a positive and clear role to both factors increase morphological, physiological, floral characteristics and reduce biochemical characteristics.7 - The effect of the triple overlap between study factors was significant, The results showed That plants sprayed kinetine And fertilization led to a significant reduction of most of the negative effects resulting from the of high concentrations of Sodium chloride in all morphological, physiological and flowering traits. Especially in the concentrations 75 ppm kinetine and in the existence of fertilization.

استخدام الزراعة النسيجية والفطر Glomusmossaes في الازالة الحيوية لعنصري الرصاص والكادميوم وتحمل الملوحة لنبات السيسبان Sesbaniarostrata L Application of Plant Tissue Culture And Glomusmossaes In Bioremediation of Lead, Cadimum And Salinity Using Sesbaniarostrata L. Plant

اسم المؤلف: التفات فاضل شحاذة الطائي
اسم المشرف: علي هاشم الموسوي كاظم محمد ابراهيم
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت تجارب عدة لغرض دراسة تحمل نبات السيسبان Sesbania rostrata L. للكادميوم والرصاص وكلوريد الصوديوم على مستوى النبات الكامل ومزارعة النسيجية. استحث الكالس واديم على وسط موراشيج وسكوج 1962 والمجهز 0.3 ملغم/لتر من الكاينتين و2 ملغم/لتر من حامض 2, 4 داي مث | Several experiments were carried out to study cadmium, lead and sodium chloride tolerance at the tissue culture or whole plant levels of Sesbania rostrata. Callus was induced and maintained on Murashige and Skooge (1962) medium (MS) supplemented with 0.3 mg/l Kinetin and 2 mg/l 2, 4 - dichlorophenoxy acetic acid (2, 4 - D) using cotyledons as the source for callus induction. Different concentrations of cadmium, lead and sodium chloride directly or gradually were added to the culture medium as contaminants. Selected tolerant cell lines were subjected to regeneration. Callus showed better tolerance to gradual exposure than direct addition. The concentrations of Potassium, lead, Cadmium and Sodium recorded to 17, 24, 27, 35 ppm when 1.0, 2.0 mg/L and 0.4% of cadmium, lead and sodium chloride were added respectively. The study included the effect of the cytokinin (benzyl adenine, BA) and the auxin (Naphthalene acetic acid, NAA) on the regeneration of shoots from callus tolerant to pollutants reached 76, 87 and 85% for callus tolerant to Cadmium, Lead and sodium chloride respectively, when the combination of 0.7 mg/l BA and 0.2 mg /l NAA was supplemented to the medium. The effect of indole butyric acid (IBA) on rooting of shoots. The concentration 0.7 mg /l gave highest percentage of rooting amounted to 66, 70 and 85% in a medium supplemented with cadmium, lead and sodium chloride respectively. Acclimatization of plantlets using river sand soil recorded 85% survival. The study was also included effects on plant height and fresh weights, shoot fresh weight was superior at the concentration 1.0 and 2.0 mg/l of cd and pb recording 8.7 and 7.5 g respectively. Treatment with NaCl led to increase the root fresh weight recording 19.1 g. Maximum cd accumulation reached 12.5 ppm in shoots, 10.5 ppm for lead in roots, 39 ppm for NaCl in roots. The experimental work investigated the effect of mycorrhiza (Glomus mosseae) on cd, pb and NaCl removal by Sesbania plant when grown in either soil contaminated with cd, pb and NaCl or non - contaminated soil (control). Inoculation with mycorrhiza led to an increase in seed germination percentage (96%), plant height (28 cm) in non - contaminated soil, while achieved an increase in shoot and root fresh weight recording 10.0 and 6.8 g respectively. Inoculation with mycorrhiza also led to a significant increase in cd and pb accumulation in roots reached 9.0 and 87.4 ppm respectively. Meanwhile, Mycorrhiza caused a significant increase in Na accumulation in root system (42.5 ppm) which already affected K concentration as a result of competition with Na recording 16.5 ppm in shoots. Mycorrhiza inoculation led to a significant increase in number of root nodes in non - contaminated soil recording 86%. Finally, mycorrhiza succeded in infecting Sesbania rostrata roots reached 90% in non - contaminated soil while it was 85% in contaminated soil

تشخيص بعض النواتج الحيوية لانواع من السيانوبكتريا المعزولة من مياه وترب مدينة تكريت ودراسة فعاليتها على بعض انواع البكتريا المرضية والحيوانات المختبرية Identification of Some Bioproducts From Some Species of Cyanobacteria Isolated From Water And Soil From Tikrit City And Study Their Biological Effects on Some Pathogenic Bacteria And Laboratory Animals

اسم المؤلف: ايمن عوني سليم جاسم
اسم المشرف: حميد سلمان خميس المهداوي احسان محمود عبد الله
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - البكتريا
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية عزل وتشخيص (19) نوع من السيانوبكتريا من عينات مياه وتربة طينية ومن الصخور من (5) خمسة مواقع في مدينة تكريت ضمن محافظة صلاح الدين، وقد تم اختيار ثلاثة انواع من هذه السيانوبكتريا وهي : Anabaena oryzae, Nostoc linckia, Anabaena laxa اعتم | The current study included Isolation and Identification of (19) species of cyanobacteria from water, clay soil and rocks samples, collected from (5) sites in Tikrit, salah Al - den province. Three species were chosen each species represent the environment which collected from it. These species were Anabaena oryzae, Nostoc linckia, Anabaena laxa. The effects of some environment factors on growth and biological products quantity has been studied in the three species above. The effects of the temperature showed that 26°C was the best for growth and biological products quantity than 16°C and 36°C. While the pH effects showed that pH 7.6 was the best for growth and biological products quantity than pH 6.6, 8.6. Three light intensities were used 1250, 2500, 5000 lux were used. The results showed that the light intensity of 2500 lux was the best for daily growth and quantity of extracted biological products. While the study of the effects of addition of macronutrient and micronutrient to Asm - 1 media which used for culturing of the three species of cyanobacteria by replacement of NaCl by NaNO3 in the medium, showed that there is an increase in daily growth and quantity of extracts of biological extracts, also the addition of FeSO4.7H2O and NaNO3 together showed further increase of daily growth and biological extracts quantity. The biological products has been identificated by HPLC - MS, IR and NMR. The results showed the presence of the neurotoxin Anatoxin - a and the hepatic toxin Microcystin in its various forms MCYST - LAba, MCYST - YM(O), [D - Asp3, Dha7]MCYST - LR and MCYST - YA in the biological products of Anabaena oryzae. The results also showed the presence of the hepatic toxin MCYST - YM(O) and [D - Asp3, Dha7]MCYST - LR in the extracts of the two spices of cyanobacteria Nostoc linckia and Anabaena laxa.The results also showed that the Muller Hinton Agar media is better than the Blood Agar media in testing the activity of these extracts in inhibition of the pathogenic bacteria, the method of holes is better than saturated discs, and the solvent DMSO (Di - Methyl Sulpho Oxide) is the better in testing of biological activities, while the filtrate of cyanobacteria did not shown any activity for inhibition of the pathogenic bacteria which include : Escherichia coli, Streptococcus pyogenes, Pseudomonas aeruginosa, Klebsiella pneumonia, Proteus Vulgaris, Staphylococcus aureus. The results showed the high capability of inhibition in concentrations of 1000, 2000, 3000 µg/ml of the extracts of the cyanobacteria Anabaena oryzae, Nostoc linckia and Anabaena laxa on the above pathogenic bacteria and some times more efficient than antibiotics used for comparison, and the concentration 3000 µg/ml was the most effective.The effects of biological extracts of the three species in concentrations of (1000, 2000, 3000) µg/kg of body weight on tested albino mice, using injection and feeding methods, was studied. The results showed poisoning nervous symptom for the first few hours, while the results showed hepatic poisoning symptom on the dissecting animals. The anatomic symptoms included changes the colour of the liver to darkening and appearance of necrosis, in addition to becoming lobbed and swollen some times with bleeding.While the poisoning effects on dissecting tissues of livers of the animals showed a simple swollen and some times assured in all of the liver accompanying with increase in growth and cell division, as well as increases in lymphatic cells inside the tissue and in general in liver tissue. The lymphocyte seemed bigger than for normal cell with multi - nuclei in white blood and increase in cell size and destruction of liver tissue. Nostoc linckia was the greater urging for cell division consequently probably more induce of cancer in the liver of the animals, while the biological product of Anabaena laxa showed more poisonous for liver cell than the biological products of Anabaena oryzae, the least effective in cytotoxic and urging cancer cell in liver of the animals.

تاثير الكلوتاثيون وبيروكسيد الهيدروجين وتداخلهما في بعض الصفات النوعية والكمية لنبات الماش Vigna radiat Effect of Glutathione And Hydrogen Peroxide And Their Interactions On Some of The Quantity And Quality Characteristic of (Mung Bean) Plant Vigna Radiata L.

اسم المؤلف: ايمان حسين هادي الحياني
اسم المشرف: وفاق امجد القيسي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Two field experiments were conducted during the spring and autum growth season of Vigna radiata L. in the year 2014 at botanical garden of Biology Department, Collage of Education for Pure Science (Ibn AL_Haithem) University of Baghdad.The experiments aimed to study the effect of glutathione and hydrogen peroxide, and their interactions on some of quantity and quality characteristic of plant Vigna radiate L.The treatment of glutathione (0, 25, 50, 75, 100) mg.L - 1 While the H2O2 (0, 5, 10, 15) ml mol. L - 1.The two experiment were designed as Randomized Complete Block Design as factorial experiment with two factors with three replication which included 60 experiment units the area of each one (1×1`) m. Results were analyzed Statistically and compared using average L.S.D at 0.05. The results of the experiments showed that glutathione revealed significantly increased all study parameter espically in 100 mg.L - 1 concentration, stem diameter by 54.56%, 28.04%, leaves number 69.34%, 53.14% for two seasons, fresh weight 44.20% for first season, the dry weight 37.43%, 91.46%, leaf area 61.61%, 151.23%.leaf area index 86.61%, 146.35%, biomass duration 60.48%, 101.06%, absolute growth rate (AGR) 32%, 92.68%, inflorescences number 56.55%, 39.90%, flower`s number 71.97%, 22.60%, root length19.82. %, 26.20%, root dry weight 76.10%, 79.25%, total function of SOD 69.32%, 40.52%, total Function of POD 29.45%, 82.25%, total Function of GPX 30.90 %, 63.62%, chlorophyll concentration of a 73.48%91.40%, chlorophyll concentration of b 35.42%, 17.67% for two seasons respectively. The total chlorophyll content 13.69%, for second season, Caroten concentration 207%, 309%, proline concentration 84.47%, 31.75%, (MDA) concentration 6.25%, 38.35%, glutathione. concentration 41.49%, 23.62 %, H2O2 concentration 52.16%, 33.24%, pods number 17.43%, 16.93%, 100 seeds weight 22.95 % 22.48%, , 93.62%, seeds yield 52.17%, 43.70%, the carbohydrate percentage 64.07%, 19.21 %, the protein percentage 22.32% for first season while the results of effect of soaking seeds with H2O2 specially with 15m ml.L - 1 concentration increased stem diameter 45.52%, 34.00%, leaves number 18.55%, 30.75%, Lateral branch 26.06%, 48.04% for Two seasons respectially, fresh weight 35.45% for first season, dry weight 40.89%, 43.85%, leaf area 53.24%, 64.53%, leaf area index 92.30%, 62.39%, biomass duration 35.35%, 57.53%, abosult growth rate34.78 %, 48%, in, inflorescences number 34.98%, 38.10% flowers number 2.987, 20.13%, root length19.44 %, 19.36%, root dry weight 99%, 96.52% total functhion of (SOD)100%, 2853%, total function of (POD) 176.57%, 40.58%, total function of (CAT)118.29%, 71.78%total functhion of (GPX)12.80%, 61.40%, Caroten concentration 54%for second season, prolineconcentration 40.93%for first season, (MDA) content 17.31% for second season, glutathione concentration, 13.68% 24.29%, H2O2 concentration 26.53%, 30.58%, number of pods 42.77%, 20.51%, seads number /pod 22.93%, 22.93%, 100 seeds Wieght 24.07%, 24.86%, seeds yield 16.69% for first season, the solubly carbohydraty percentage 43.26%for first season, the protein percentage 11.50%, 25.18 % for two seasons respicatlly, while chlorophyll a decreas concentration ed by 15m ml.L1 12.87%for second season, total chlorophyll concentration 39.66%, 26.22 for two season respicatlly. There were high significant interaction between glutathione and H2O2 in all study parameter characters in the two experiments.

تقييم نوعية مياه نهر الفرات (بين مدينتي الكفل والشنافية) وسط العراق Assessment of Water Quality of Euphrates River (Between The Cities of Al - Kifl And Al - Shinafiyah) Iraq

اسم المؤلف: دنيا باهل جدعان صلال الغانمي
اسم المشرف: حسين يوسف خلف الركابي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: البيئة المائية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية على جزء من بيئة نهر الفرات الممتد بين مدينتي الكفل والشنافية لمدة عام تقويمي من ايار 2013 ولغاية نيسان 2014 واختيرت اربعة مواقع مختلفة وموزعة على منطقة الدراسة شملت ثلاث محافظات هي : بابل - النجف - القادسية (الفرات الاوسط - العراق)، | The current study has applied on the Euphrates River for a full year from May 2013 until April 2014. Four sites selected along the river whithin three provinces; Babylon, Najaf, Al - Qadisiyah. Assessment of water quality done by using Canadian Council of Ministers of Environment (CCME) and Index of Biological Integrity (IBI) for the purpose of determining the validity and quality of its water for the General purpose and for Drinking and Irrigation.The physicochemical properties and biological (Phytoplankton) were studied during the study period. These properties were; temperature of the air and water, light penetration, electrical conductivity, salinity, turbidity, water current speed, total suspended and dissolved solids, dissolved oxygen, biochemical oxygen demand, chemical oxygen demand, carbon dioxide, total alkalinity, acidity, total hardness and ions of calcium, magnesium, sodium, potassium, and the sodium adsorption ratio, chlorides, sulfates, boron, nitrite, nitrate, phosphate, silica and some heavy metals such as cadmium, lead, zinc, copper, chromium, manganese in both phases : dissolved and particulate. results showed that the values of the physical and chemical factors ranged as follows : (7 - 47) C, (10 - 31) C, (14 - 177.5) cm, (1020 - 5600) µs/cm, (0.65 - 3.46) 0%, (0.07 - 129) NTU, (27.03 - 115.57) cm/s, (591 - 2985) mg/L and (0.001 - 0.128) mg/L, (7.04 - 8.8), (6 - 11.8) mg/L, (0.13 - 6.6) mg/L, (8.2 - 53) mg/L, (81.4 - 198) mg/L, (30 - 200) mg/L, (6 - 46) mg/L, (490 - 929.67) mg/L, (83.37 - 290.18) mg/L, (0.79 - 174.84) mg/L, (92.5 - 713) mg/L, (1.9 - 11.18) meq/L, (6 - 133) mg/L, (97.97 - 861.73) mg/L, (329 - 1040) mg/L (0.01 - 4.23) mg/L, (0.008 - 1.33) µg/l, (2.16 - 44.74) µg/L, (0.012 - 0.59) µg/L and (104 - 514) µg/L respectively.Concerning the heavy elements, the values of dissolved cadmium ranged between (undetectable - 0.075 mg/L) and it's particulate between (0.0043 - 0.2705) mg /L and dissolved lead between (undetectable - 0.0232 mg /L) and it's particulate between (0.0045 - 0.148) mg/L and dissolved zinc between (un - detectable - 0.0168 mg/L) and it's particulate between (0.0115 - 0.316 mg/L) and dissolved copper (undetectable - 0.0273 mg/L) and copper is particulate between (0.0062 - 0.063 mg /L) and dissolved chromium between (undetectable - 0.0193 mg/L) and the chromium is particulate between (0.0043 - 0.0449) mg/L and dissolved manganese (undetectable - 0.0256) mg/L and it's particulate between (0.0085 - 0.0739) mg/L.The biological tests include Qualitative and Quantitative Study on phytoplankton and also included calculating the total number of coliform way casting dishes by using the MacConkey Agar media.The number of diagnosed species of Phytoplankton was 295 species, belonging to 74 genera. The Diatoms Bacillariophyceae won the biggest part of it as it formed 160 species and 35 genera any rate (54.24%), followed by green algae (70 species belonging to 24 genera) and green algae (52 species belonging to 11 genera) then Euglenophyceae (10 species belonging to both sexes), while golden brown algae and algae Pyrrophyceae were less algal groups registered as belonging to the two single - sex. Some types of phytoplankton existed in most of the duration of the study, including Bacillaria paxillifera, Cocconies placentula, Cyclotella meneghiniana, Diatoma elongatum, Fragilaria virescence, Melosira ambigua, Synedra acus.The total number of phytoplankton is estimated between (104.9 - 4460.09) cell × 103/liter. An increase in the number of phytoplankton in July and January was observed clearly. The concentrations of chlorophyll - a were ranged between (0.0693 - 9.78 mg /L). While the total number of results for coliform were (5 - 281 × 103) cells/liter, The water quality of the evidence Account (Canadian model) using a number of physical, chemical and Biological properties. The results showed that the waters of the Euphrates are located within categories (Poor - Good) with respect to the public evidence of the quality of water of this river while it was located within categories (Poor - Acceptable) with respect to their use for drinking, While its use for irrigation purposes, it was clear that run river water data fall into two categories (Poor - Good). The results of the analysis of the main components Principal component Analysis (PCA) indicate that the nitrates and the total number of coliform and dissolved oxygen, phosphate, zinc, lead and Biological oxygen demand and pH are the biggest impact on the water quality of the index values of the variables of general purpose.These factors are responsible for the deviation index values, followed by turbidity and total hardness and electrical conductivity and temperature of the water, copper, chromium, and cadmium heat. But with the biggest impact of the index water quality factors for drinking purposes are chloride and electrical conductivity and total hardness, magnesium, turbidity and basal college, sodium, calcium, copper, sulfates, nitrates and coliform, zinc and dissolved oxygen, pH, manganese and lead followed by boron nitrite, cadmium, chromium and vital requirement for oxygen. While the chloride and conductivity electrical, the proportion of sodium, copper, zinc and adsorption of boron have greatest influence on the value of water quality index for irrigation, followed by manganese, cadmium, chromium, lead and pH.The phytoplankton index of Biotic Integrity (P - IBI) has been calculated using ten units including (relative abundance of green algae, green algae, pyrrophyta, Euglenophyta, Xanthophyta, Centralales, pennales, species richness index, the density of phytoplankton and chlorophyll a) The results showed that phytoplankton index of Biotic Integrity values ranging between (47 - 74) in the first position and (47 - 67) in the second location, and (45 - 76) in the third site and (45 - 71) in the fourth site. In other words, phytoplankton index of Biotic Integrity with estimating index (good - poor). The results showed the effectiveness of phytoplankton index of Biotic Integrity values in estimating the environmental changes.

علاقة مستوى الزنك والنحاس ببعض المعايير المناعية في الاصابة بداء المقوسات في النساء في محافظة كركوك مع محاولة علاجية في الفئران المختبرية Relation of Zinc And Copper Levels With Some Immunological Markers in Women With Toxoplamosis in Kirkuk Governorate With Trial To Treatment in Mice

اسم المؤلف: عبير عباس علي
اسم المشرف: حسين ساهر اسود العبيدي شهاب احمد محمد
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: تم دراسة 100امراة مصابة بداء المقوسات Toxoplasmosis الذي يسببه الطفيلي المقوسة الكوندية Toxoplasma gondii للفترة من اذار 2007 ولغاية نيسان 2008 راجعن العيادة الاستشارية التابعة لمستشفى كركوك العام في مدينة كركوك. اظهرت نتائج الدراسة الحالية ان 56 % من الن | A total of 100 patients suffering from Toxoplasmosis which is infected by the parasite Toxoplasma gondii have been dealt with as subject of the sample during the period from March 2007 to April 2008 at the Consultant Clinic of Kirkuk General Hospital. The results of the present study showed that 56% of the subjects were pregnant and 44%were not. The highest percentage of the infected subjects was of 26 - 30 years old as 30.37% among the pregnant women and 36.35% among the non - pregnant women of the age mentioned above. The highest percentage 37.5% of abortion of the first period of pregnancy was in pregnant women, and 45.53% in non - pregnant women. The results of the study also showed more cases of infected women among those who bred animals in their living places as 69%. It is also found that the highest percentage of the infected women 64% were of those who use the running water for drinking. Moreover, it is noticed that there is an increase in the percentage of the infected women among the uneducated women 38%. The study showed that the percentage of the infection is proportional to residence 63%, the infection increase among women living in rural areas. The percentage of T - lymphocytes in peripheral blood of patients were also measured through measuring the percentage of the CD4+ and CD8+cells by using fluorescent microscope.The results showed a significant decrease (p<0.05) in this type of cells.The rate of percentage of CD4+ of the patient's were 31.704±0.116 while the rate of CD4+ in the peripheral blood of the control group was 62.260±0.237.The rate of percentages of CD8+ 19.382±0.080, whereas it was 31.680±0.307 of the control group.The rates of the percentages of CD4+ to CD8+ were also measured, the results showed a significant decrease (p<0.05) in the rate of the percentage in the patient's group 1.642 while it was 1.968 in the control group. The statistic analysis showed significant differences between these tow groups (p<0.05). The intensity of Zinc and Copper has been measured in the serum of the infected women via use of Atomic absorption instrument, a significant decrease (p <0.05) appeared in the rate of Zinc intensity in the serum on the infected women as compared with the control group.The rate of the intensity in patient's group was 4.65% ± 2.628µmol /l while in the control group it was 15.353± 1.640 µmol /l.A significant increase (p<0.05)was also noticed in the intensity of Copper in the patient's group (28.589±4.335 µmol /l)whereas it was 12.854±1.501 µmol /l in the control group. The index of the specific antibodies IgM and IgG related to Toxoplasma gondii was measured by ELISA method, a significant increase (p<0.05) was observed in the value of IgM and IgG in the patient's group as compared with the control group. The rate of index for IgM in the patient's group was 1.354±0.05 while it was 0.163±0.05 in the control group. The rate of value of IgG in the patient's group was 1.163±0.074 whereas it was 0.358±0.033 in the control group. In the present study, Toxoplasma gondii was isolated and diagnosed from human as first attempt, and then the infection was created in laboratory mice as an essential step for growing the parasite. The second step was studying the effect of some selected drugs on the experimental treatment for disease of toxoplasmosis created in the mice. The drugs are : Pyrimethamin, Clarithromycin, Spiramycin, Ivermectin.The results of the study showed the effects of these drugs on the tissue cyst formed in the brain and lungs. The number of tissue cysts in the brain of the mouse treated with pyrimethamin was 14.8 ±1.067 after 28 days. Significant differences appeared when this result compared with the control group in which it was 27.4±1.029. The number of tissue cysts formed in lung, tissue in the mouse treated with the same drug after the same period of the time was 6.2±0.583.This showed a significant differences as compared with the control group in which it was 10.2±0.583. No significant differences appeared between both groups as for the treatment by Clarithromycin antibiotic as far as the number of the tissue cysts formed in the brain after 28 days is concerned. The number was 23.8 ±0.86 as compared with the control group 38.6±1.029.The same compared is true of the lungs, tissue. The number of the tissue cysts was 23.8±0.86 as compared with the control group 28.4±1.805. Concerning the antibiotic Spiramycin, the results showed the effectiveness of it in decreasing the tissue cysts formed in the brain.The significant differences were (p<0.05), the rate of the cysts was 9.6±0.6 as compared with the control group in which it was 22.8±1.067.The same effectiveness appeared on the lungs, tissue as the rate of tissue cysts after 28 days of treatment was 4.6±0.509 as compared with the control group in which was 17±1.14 and the significant differences were (p=0.000).The Ivermectin drug showed a great effectiveness in minimizing the number of the tissue cysts formed in the brain and the lungs. The numbers of the tissue cysts formed after 28 days were 3.2±0.374 and 1±0.316 respectively; significant differences are observed when these numbers are compared with the numbers of the control group which were 22±1.843 and 19.4±1.435. Thus, the use of Ivermectin drug is considered as a pioneer and fruitful study since this drug has not been used for the treatment of Toxoplasmosis.

تحديد تجمعات Giardia lamblia ومعقد Entamoeba histolytica / Entamoeba dispar بالتشخيص الجزيئي (PCR) للمصابين في تكريت Determination of Giardia Lamblia Assemblages And Entamoeba Histolytica / Entamoeba Dispar Complex By Molecular Diagnosis (Pcr) in Patient in Tikrit

اسم المؤلف: انتصار غانم عبد الوهاب الصميدعي
اسم المشرف: شهاب احمد محمد الجبوري عقيل حسين العاصي
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة للمدة من كانون الثاني 2011 ولغاية كانون الاول 2011 للمرضى الراقدين والمراجعين لمستشفى تكريت التعليمي وبعض المختبرات الخاصة في مركز المدينة وضواحيها والذين يعانون اعراض مرضية مختلفه ولفئات عمرية مختلفة ترواحت بين اقل من سنة الى 61 سنة، ح | This study was conducted for the period started from January 2011 until December 2011 on inpatients and outpatients of Tikrit educational hospital and some private laboratories at Tikrit city and surrounded suburbs. Symptoms of different diseases among various age groups varies between less than one year to 61 years. The tests used the (ELISA & PCR) and the analysis of the PCR - RFLP to discriminate the infection by assemblage of Giardia lamblia and differentiate between pathogenic amoeba Entamoeba histolytica and nonpathogenic Entamoeba dispar compared with microscopical examination. The result of present study showed that (75) person who represent (37.5%) were infected by Giardia lamblia and (75) person who represent (37.5%) were infected by E.histolytica/E.dispare complex, out of (200) stool samples which have been tested by microscopical examination. Epidemiological study showed no significant difference among the sex for the two species parasites whereas a high percentage for infection recorded in group at age (1? - 10) year for Giardia lamblia and E.histolytica / E.dispar complex reach to (60%, 36%) respectively. Regarding the place of living Giardia lamblia in rural area showed infection rate of (19.5 %), while infection rate by E.histolytica/E.dispar complex reaches (22.5%) in urban area. Statistical analysis shows a high difference of significance according the source of water supply. Tap water showed high incidence for initiating infection which reaches to (93.3%) by G.lamblia and (90%) for E.histolytica/E.dispar complex. For the infection rates and their relationship to the educational level, the results show that the highest rates of infection recorded in non - educated (52%) and (73.3%) of Giardia lamblia and E.histolytica / E.dispar complex, respectively. Depending on the sample texture, the result show that oily diarrhea is more correlate with infection reaches to (33.3%) of Giardia lamblia and the watery diarrhea form 40% by E.histolytica / E.dispar complex, while simultaneous occurrence of both symptom (colic and loss of appetite) are the most symptom synchronized with infection records (44%) for Giardia lamblia and (36%) for E.histolytica / E.dispar complex. The percentage of infection by Giardia Lamblia in this study depends on the test results of the ELISA was (20%), while the percentage of infection E.histolytica/E.dispar complex was (6.7%). Testing has shown sensitivity compared to microscopic examination reaches to (22.6%) and (8%) for both parasite respectively, while the test showed a high specificity which reaches the rate (93.3%) and (100%) for both parasites respectively. Result of PCR showed a percentage of infection by E.dispar about (32%) (64 sample) from the total samples, whereas it does register any cases infection by E.histolytica. By counting the results of the PCR for the three genes (ssu rRNA + TPIA + GDH) they show a total infection by Giardia lamblia which was (10.5%) (21 samples). This study recorded percentage of infection Giardia lamblia depending on the result of the three Genes separately amounted to (4%), (5%), (3%) for (ssu rRNA, TPIA, GDH) respectively. According to the result of the analysis PCR - RFLP for GDH gene by using the enzyme NIaIV, it did not record any infection by Giardia lamblia (assemblage A), whereas (6 samples) recorded (100) by Giardia lamblia (assemblage B), by using the enzyme Rsal to determine the secondary assemblage shows that the total (6 samples) infected there are 5 samples carrying infection by two type of secondary assemblage returning to the assemblage B (assemblage BIII +BIV) and only one sample infected with (assemblage BIV). To determine the assemblage and secondary assemblage for the parasite result compared with amplification of TPIA gene with lysis by enzyme for the resulting amplification for GDH gene, resulting a ratio of (14.3%) for a sample infection by both assemblage (A+B) and secondary assemblage (BIII+BIV) together, and a ratio of (57.14%) infection by assemblage A only and (21.4%) infection by secondary assemblage (BIII+BIV) together and (7.14%) infection by BIV only.

التحري عن طفيلي المقوسة الكوندية Toxoplasma gondii في الطيور الداجنة في منطقة الفرات الاوسط والقطط في محافظة الديوانية باستخدام التقنيات المصلية والجزيئية Detection of Toxoplasma Gondii In Domestic Avian In Middle Euphrates Region And Cats In Al - Diwania Province By Serological And Molecular Techniques

اسم المؤلف: خديجة عبيس حمود الخالدي
اسم المشرف: خيري عبد الله داود العكيلي هادي مدلول الميالي
الموضوع العام: علوم الحياة
السنة: 2013
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية الكشف عن طفيلي المقوسة الكوندية Toxoplasma gondiiفي اربع انواع من الطيور ضمت الدجاج المحلي Gallus gallus domesticus والديك الروميMeleagri galbpavol والاوز الاربد Anser anser والبط المحلي Anas platyrhychos domesticus في منطقة الفرات ا | The current study was conducted to detect of Toxoplasma gondii in five species of avian including, Gallus gallus domesticus, Meleagris galbpavol, Anser anser, Anasplatyrhychos domesticus in middle Euphrates provinces of Iraq, also in cats in AL - Diwania province, during 2011 - 2013 by using serological tests (Latex, Rapid test cassette) and molecular test (conventional - PCR and Nested - PCR) as confirmative tests to detect of B1gene which was specific gene of T.gondii in blood and Tissue samples of different organs such as Liver, Heart, Brain, Eye, Lung, Pectoral muscles, Small intestine, Spleen, and Leg muscles of avian and cats which were seropositive cases of Rapid test cassette, also the study was included the detection Oocysts of T.gondii in soil samples of ten cities from Al - Diwania province by using sedimentation and floatation methods as primarily diagnosis, also using the polymerase chain reaction to detect of B1gene in these samples. A total of 320 Avian collected, include 80 samples of each species (20 samples of chicken, Turkey, geese, and Ducks) of each province of middle Euphrates (AL - Diwania, Babylon, AL - Najaf and Karbala), in addition to 10 samples of cats and 100 samples of soil from the center of AL - Diwania province, samples of avian and cats were involved to serological test such as LAT, the results showed there were 96 positive samples in percentage %30, include, 31(%38.75) of chicken, 28 (%35) of turkey, 22 (%27.5) of geese, 15 (%18.75) of ducks and 5 (%50) of cats, the higher Percentage (%23.95) of infection with T. gondii in avian was in titer 1/160 and the lower percentage (%3.73) in titer 1/640, Whereas in cats, the higher percentage in titer 1/20 and lower percentage in titers 1/40, 1/80, 1/160 which reaches %20. The results of Rapid test cassette were showed there were 60 out of 320 samples in percentage %18.75, include 24 (%30) of chicken, 19 in (%23.75) of turkey, 10(%12.5) of geese, 7(% 8.75) of duck and 3(%30) of cats. Also the results of Rapid test cassette were showed the higher percentage of types of antibodies in the serum of avian was %36.66 of IgG +IgM together and the lower percentage was %28.33 of IgM, whereas in cats, the higher percentage was %66.66 of IgG and the lower percentage was %33.34 of IgG +IgM together. To confirm the infection of avian by T.gondii, Polymerase Chain Reaction was used to detect the B1 gene (399 bp) in 320 blood samples of avian and 10 samples of cats, the results were showed there were 34 samples (%10.62) of avian gave positive result, include, 14 (%17.5) of chicken, 12 (%15) of Turkey, 8 (%10) of geese, whereas the results were showed the B1 gene was not present in the blood samples of ducks and cats. The results of Latex, Rapid test cassette and polymerase Chain Reaction were showed, the higher percentage was recorded in chicken % 17.5, %30, %35, whereas the lower percentages were %18.75, %8.75, %0 in D ucks by using the three tests, respectively. According to the results of Rapid test cassette (IgG, IgM +IgG), the results of polymerase Chain Reaction to detect the B1 gene in 16 samples of avian (4 samples of each species) out of 39 samples and 3 samples of cats, showed there were 9 samples (%56.25), include 4 (%100) of chickens, 3(%75) of turkey, 1(%25) of geese, and 2(%66.66) of cats and the higher percentage of B1gene (399bp) in livers of avian was %55.55, %22.22 in pectoral muscles then heart, spleen and small intestine in percentage %11.11 for each one of them, whereas in cats the percentage were % 66.66 in liver while in brain and kidney the percentage was %33.34 of each one of them.. The results of Nested Polymerase Chain Reaction for amplification the gene B1 in 12 blood samples (4 samples of each species of birds except ducks) was positive for conventional - PCR, Showed the amplification occurs in 4 samples in percentage % 33.33 during the first round of reaction and 3 samples in percentage %75 in the second round, in 10 samples of tissues, the amplification occurred in 6 samples in percentage %60 during the first round and in 4 samples in percentage % 66.66 during the second round of reaction. Also the results were showed the percentage of T.gondii antibodies in serum of four types of avian (except pigeons) according to the provinces were %28.75 in AL - Diwania, %40 (Higher percentage) in Babylon, %12.5(Low percentage) in AL - Najaf and %38.75 in Karbala by latex test, whereas the percentages %26.25 (higher percentage), %10 (lower percentage), %12.25, %17.5 in each of Karbala, AL - Najaf, Babylon, AL - Diwania province respectively by Rapid test cassette, and by using polymerase chain reaction, the percentages were %16. 25 (Higher percentage), %5(Lower percentage), %6.25, %15 in AL - Diwania, Babylon, AL - Najaf and Karbala province, respectively. Also the results showed, the percentage of infection in AL - Diwania province the samples of rural areas were higher (%37.5, %22.5, %17.5) whereas the percentages were lower (%20, %12.5, %12.5) in urban areas by using latex, rapid test cassette, and polymerase chain reaction, respectively. The results of comparison between two serological tests and Polymerase Chain Reaction, showed the percentages of infection were % 10.62 (34), %18.75 (60), %30 (96) by using Polymerase chain reaction, Rapid test cassette, latex test, respectively, also the sensitivity and specificity of latex test were %35, %72 whereas the sensitivity and specificity of Rapid test cassette were %35, %88, respectively in compared with standard Polymerase Chain Reaction. In the soil samples, the percentage of presence of Oocysts of T.gondii was %10 by using floatation method and %4 by conventional Polymerase Chain Reaction. The study of grossly and microscopy pathological changes in avian and cats which infected with T. gondii, was showed there were lesions on each of liver, heart, pectoral muscles, small intestine, brain and kidney.

دور اجهاد المضاد الحياتي (سيفتازديم) في امراضية الممرضة البولية الايشريكية القولونية المنتجة للبيتا لاكتاميز موسعة الطيف في الفئران المختبرية Role of Antibiotic (Ceftazidim) Stress On Pathogenicity of Uropathogenic Extended Spectrum ? - Lactamaes E. Coli In Laboratory Mice

اسم المؤلف: علي حسون حمادي عبد الله
اسم المشرف: محمد قيس العاني حارث جبار فهد
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: الانبار
الصفحات الاولى:
المستخلص: جمع 60 نموذجا من عينات بول وسط المجرى من المرضى الراقدين في المستشفى الذين يعانون من اخماج المجاري البولية من مستشفى اليرموك ومستشفى الكرخ ومستشفى القديس رافائيل/ الراهبات للمدة من 27 شباط 2014 ولغاية 25 اذار 2014، وقد اظهرت نتائج الزرع البكتريولوجي الحصو | Sixty mid - stream urine specimens were collected from patients presented with urinary tract infections whom attending Al - Numan, Al - Yarmouk, Al - Karkh, and Saint Raphael/Al Rahibbat hospitals in Baghdad for the period February, 27th to March, 25th 2014. Bacteriological culture identified fifty (80.33%) isolates as E. coli depending on cultural and biochemical characteristics as well as Api and Vitek 2 automated systems. However, 82% of isolates developed ? - hemolysis on blood agar plates.Susceptibility of all isolates against seven commonly prescribed antibiotics were investigated and 96, 82, 84, 92, 92, and 56%. of isolates were resistant to ampicillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, cefalothin, imipenem, and meropenem, ceftazidime, respectively.Concomitantly, ? - lactamase production was detected phenotypically by disk replacement method. The results showed that 82% of all isolates have the ability to produce ? - lactamase. Interestingly, correlation coefficient between ? - lactamase and hemolysin production was 0.99.Using microtiter plate method, all E. coli isolates were tested for their ability to form biofilm. The results revealed that 36, 54, and 10% of isolates formed biofilm with variable thickness; weak, moderate, and strong, respectively.Alongside with previous findings, blaCTX - M - 2, blaVIM, blaTEM, and blaPER were identified in 18, 12, 10, and 4, respectively. Nevertheless, none of blaOXA - 1, blaOXA - 4, blaOXA - 30, blaIMP, blaCTX - M - 1, blaCTX - M - 9, blaKPC, and blaSHV. The isolate number 11 harboured the highest number of genes (blaTEM, blaVIM, and blaPER) among all tested isolates. Twelve albino mice was divided randomly into four groups comprising A through D injected with ceftazidime at sub MIC, E. coli 11, E. coli 11 with ceftazidime solution, and standard strain, respectively.Histopathological sections did not show any changes in respeuct to group A. however, group C suffered signs of infection less than those appeared in group B sections. Simultaneously, group D suffered intense histpathological changes more than other groups infected with resistant isolate.

دراسة علاقة سم Ochra. A بحالات الفشل الكلوي غير معروف الاسباب في محافظة القادسية Study The Relationship of Ochra.A With Kidney Failure of Unknown Case In Al - Qadisiya Governorate

اسم المؤلف: هدى رحيم هاشم الموسوي
اسم المشرف: بهيجة عبيس حمود الخالدي
الموضوع العام: علوم الحياة
السنة: 2015
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة لتحديد العلاقة بين السموم الفطرية وحالات الفشل الكلوي غير معروف الاسباب لدى المرضى المراجعون الى مستشفى الديوانية التعليمي للفترة من اذار/2012 الى كانون الثاني/2013 والذين تم تشخيصهم من قبل الاطباء المختصون. اظهرت نتائج فحص عينات الادرا | This study was designed to detection the Relationship between some fungi and it’s toxin with Renal falier in patients suffer from Renal falier attended to Al - Diwanyia teaching hospital in period from March/ 2012 to January/ 2013, These patients were diagnosed by physician in above hospital. By using Thin Layer Chromatography (TLC) the results showed 23.07% from patients blood specimens had Ochratoxin A, 19.23% from patients urine specimens had Ochratoxin A 38.46% from patients had Ochratoxin A in blood and urine where only 59.61% your blood and urine were clear from above toxin, So healthy persons were had Ochratoxin A in your blood and urine by rate 20% 40% respectively and 10% from them had Ochratoxin A in your blood and urine and only 65% from them don’t had Ochratoxin A in their blood and urine.the concentration of Ochratoxin A in blood were ranged between 6.2 - 25.8 ?g/kg from weight boody and 4 - 10.3?g/kg in urine and in healthy reach concentration of Ochratoxin A in blood 2.3 - 3 ?g/kg from weight boody and 4.3 - 4.6 ?g/kg in urine.The data of this study showed the male highly infected 87.5% with ocratoxin while in female 70% and 71 - 80 years age had highly infected 92.85% with ochratoxin A. The results of study of biochemical parameter in patients showed highly raised in WBC and urea and Keratinin to reach to 12000 corpuscle /ml3 310 mg/dl, 12.2 mg /dl respectively and lowed the activity of alkaline phosphates enzyme and albumin into 2.2 u/l, 1.5 gm/dl, respectively and suger 120 mg /dl. The results of isolation and diagnosis by depending on Morphological, Microscopical and Biochemical characters showed there were two species of fungi belong to Aspergillus sp, include A. ochraceaus 25.42%, A. niger 12.71% and A. candidus 15.25% and Candida albicans 46.61% were isolated from urine patients where only C. albicans and C. krusi were isolated from blood of patient by percentage 66.66% and 33.33% respectively. So the results of single PCR method selected to detection the OCR and PSK genes in Aspergills spp which responsible for ochratoxin A production , showed 83.33% from A. ochraceaus isolates had OCR gene (907 bp) and 66.66% from A. nger had PSK gene (584bp)Also the results of this study showed A. ochraceus and A. niger had ability to produce metabolic compounds when grow on yeast - extract broth and these compounds had significant toxic effects(P?0.05) in biochemical parameters in albino rats represented raised number of white blood cells (WBCs) into 10610 cell/µm3, urea into 228 mg/dl and Keratinin into 15 mg/dl in blood of animals which treated with 5000 ?l/kg from metabolic products of above fungi respectively, comparative with control groups 10100 cell/µm3. in other side, these metabolic products caused in same dose lead to lower of albumin and activity of alkaline phosphatase enzyme into 2.8 mg/dl and 3.7 u/l respectively where normal level for this parameter in control groups.Histological study showed the dose of metabolic products 500 - 5000 µl/kg for both fungi caused pathogenic changes in kidney represented by, atrophy highly proliferation of cells in endothelial layer of Bowman's capsules in addition to hemorrhage and Focal agrgregate inflammatory cells.In other side, study of toxicity of ochratoxin A which extracted by using thin layer chromatography (TLC) teqnique from urine and blood of patients and yeast extract broth media that had growth of A. ochraceus and A. niger showed signification effects(P?0.05) for this compound in physiological and biochemical parameters also caused significant effects in histological parameters similar to these effects which occurred in previsious experiments while these effect don’t appear in control groups.

تاثير الاجهاد المائي والسماد الورقي والمخصب العضوي في نمو نبات حلق السبع الشجيري Adhatoda vasica (L.) Nees ومحتواه من بعض المواد الفعالة وفعاليتها المضادة في بعض بكتريا تسوس الاسنان Effect of Water Stress, Foliar And Organic Fertilizers On Growth of Adhatoda Vasica) L.(Nees. And Its Content of Some Active Ingredients And Inhibition Activity of Some Dental Caries Bacteria

اسم المؤلف: ليث سريع الركابي
اسم المشرف: ثامر خضير مرزة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: This study was conducted in Biology Department - College of Science - University of Al - Qadisiya that included pot experiment from the period of 1/4 to 1/8/2013, to assess the effect of water stress, foliar fertilizer (Foltron) and organic fertilizer (Azomin) on growth of Adhatoda vasica (L.) Nees and Its Content of Some Active Ingredients. Laboratory experiments were also done from the period of 1/8 to 1/3/2014, to determine the activity of leaves and roots extracts that resulted from plants of pots treatments to inhibit growth of some bacteria genera causing Dental Caries i.e. Staphylococcus aureus, Streptococcus mutans and Streptococcus pneumonia.A pot experiment included seventy two transplants (age of one month and 8 cm height) that were transplanted in plastic pots (one transplant per pot) in 1/4/2013. Pots were filled with sandy soil and peatmose 2 : 1 v/v. Plants Irrigated with two levels 50 and 25% of field capacity. Foliar fertilizer was used at three concentrations of 0, 3 and 6 ml/L that sprayed twice on plant shoot till complete wetness during 15/4 and 1/6/2014. Organic fertilizer was also used with irrigation water at three concentrations of 0, 4 and 8 ml/L twice on 1/5 and 15/6/2014. The design of the experiment was Randomized Complete Blocks Design (RCBD) in a factorial arrangement (2×3×3) with four replications per each treatment. Revised Least Significant Difference (RLSD) was used to compare treatment means at probability 0.05 level when treatment effect was significant.Results of pots experiment were as follows : 1 - Increasing water stress level caused a significant decrease in the most characteristics of vegetative and root growth except for stem internode and root lengths, while increasing in concentration of foliar and organic fertilizer gave the opposite effect of water stress.2 - Water stress caused a significant decrease in leaves content of total chlorophyll and nutrient elements N, P, K, Mg and protein percentage, while foliar and organic fertilizer caused a significant increase on the above characteristics.3 - Water stress caused a significant increase in proline percentage and Vit.C. and Vasicine and Vasicinone alkaloids, while foliar and organic fertilizer caused a significant decrease in proline percentage and Vit.C. and a significant increase in Vasicine and Vasicinone alkaloids.4 - The treatment of plants that were subjected to the water stress via foliar or root fertilizing or both of them caused a significant increase for most of vegetative and root growth characteristics, total chlorophyll, nutrient elements, protein percentage and Vasicine and Vasicinone alkaloids.5 - The treatment of plants that were underwent to the water stress via foliar and root fertilizing caused a significant decrease in proline percentage, Vit.C. and stem internode and root lengths.6 - The interaction of the three studied factors gave a significant positive effect to rise alkaloids of plant.Laboratory experiments included picking fresh leaves and root of plants that dried aerial in a shade, and then extracted it via methanol alcoholic of 70%, it was detected for some chemical components of extract by using reagents. Bacterial genera were isolated from the surfaces of the teeth and cultured on the growth media, and it was diagnosed by the Vitek Compact 2 System Diagnosis. The activity test involved the impact of the alcohol extract for leaves and roots to the inhibition of bacterial growth by the agar well diffusion method on Muller - Hinton.The design of the experiment was Complete Randomized Design (CRD) in a factorial arrangement (18×3) with three replications per each treatment. Revised Least Significant Difference (RLSD) was used to compare treatment means at probability 0.01 level when treatment effect was significant.Results of laboratory experiment were as follow : 1 - Increasing extracts concentration caused a significant increase in growth inhibition of the studied bacterial genera.2 - plants extraction that of the combination of A2B3C3 included (25% water stress, 6 ml/L foliar fertilizer and 8 ml/L organic fertilizer) gave the highest activated inhibition for bacterial genera compared with plant other extraction for all tested bacterial genera.3 - The plants extracted that treated by combination A2B3C3 in concentration 150 mg/ml gave higher activated inhibition for Streptococcus pneumonia reached 25.307 ml compared with Amoxicillin that reached 36.167 ml, while the inhibited extracted root reached for the same treatment 16.060 ml (Table 37 and 38).4 - Plants leaf extract was the highest effective inhibition of bacterial compared to the roots extract.

معالجة مطروحات مياه فضلات الصرف الصحي لمدينة تكريت باستخدام تقنية المجففات الحرارية الدواره واعادة استخدامها كاسمدة عضوية Treatment of Domestic Wastewater Effluent of Tikrit City Using Thermal Rotating Dryer Technique And Reuse As Organic Fertilizers

اسم المؤلف: هالة ارشد علي حسين
اسم المشرف: جهاد ذياب محل الجنابي
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: البيئة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • البيئة - التلوث البيئي
الصفحات الاولى:
المستخلص: اجريت الدراسة في مختبرات كليتي التربية والعلوم - جامعة تكريت للفترة من نيسان 2010 لغاية شباط 2011 تتضمن الدراسة معالجة الحماة الناتجة عن حوض التجميع الابتدائي باستخدام تقنية المجففات الحرارية الدوارة للعمل على ازاحة ماء الحماة والتي تمثل اصعب العمليات الت | This study was conducted at a period from April 2010 to February 2011, to treat the sludge produced from collection tank at tikrit domestic wastewater plant, using thermal rotating dryer technique(T.R.D) or dewatering the sludge.This process represents one of the difficult applied process for the sludge treatment. This technique considered a modern technique in this field.Physical, chemical and biological characteristics of the manufactured sludge were determined and compared with organic fertilizers (I.O.F) imported from republic of Estonia (Available in local markets).T.R.D technique induced dewatering of the treated sludge at ratio48.5 - 61.5% and producing highly sterilized organic fertilizers which was free of pathogenic organisms(bacteria, worms and eggs) with high chemical characteristics comparing with those imported from Estonia. Manufactured organic fertilizers M.O.Fs have allow acidity pH ranged between 7.5 - 7.7 ,. electrical conductivity at range 1630 - 1873, organic matter at ratio 4.56 - 5.35%, with a moderate concentrations of Pb, Cd, Zn.The concentration of N and K was 1.8, 5.96% respectively while the concentration of phosphorus are 350 ppm. Three levels of M.O.Fs were used for corn plants(5, 10 and 20ton/acres). Results showed that all these levels significantly induced increasing for some physiological and morphological characteristics of corn plants compared with those treated by I.O.Fs or control plants. The vegetative dry weight of corn plants fertilized by M.O.Fs were 23.95gm/plant compared with 16.3 and 5.3 gm/plant for plants fertilized by I.O.Fs and control respectively. (M.O.Fs) induced significant increase in nitrogen concentration compared with plants fertilized by I.O.F and control while the concentrations of Pb, Cd, Zn were not affected by using of M.O.Fs on the other hand Pseudomonas aeruginosa and Escherichia coli were contributed in reducing and removing some of pollutants from the sludge at three incubation periods (30, 60 and 90 day). These two species caused significant decrease in pH and EC of the sludge, P. aeruginosas showed the highest removing ratio of nitrogen (93.1%). The cooperation of the two species caused high removing ratio for the Total organic carbon(87.72%) at 60 days and organic materials (90.68%) at 90 days of incubation also showed high removing ratio for phosphors 100%) at 90 days of in cubation.) These two species also caused high removing ratio for pb, Cd and Zn at30 days and P. aeruginosa was more effective in removing Pb, Cd and Zn from the sludge then E. coli.

تاثير الري بالماء المعالج مغناطيسيا في نمو وحاصل الحنطة Triticum aestivum L Effect of Irrigation With Magnetic Treated Water On Growth And Yield of Wheat (Triticum Aestivum L.)

اسم المؤلف: فاضل كاظم كريم الابراهيمي
اسم المشرف: ثامر خضير مرزة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: نفذت التجربة في حقل تابع للمعهد الفني - الكوفة شمال غرب محافظة النجف لموسم النمو (2012 - 2013) بهدف معرفة تاثير نوعية مياه الري وشدة معالجتها مغناطيسيا لثلاثة اصناف من حنطة الخبز Triticum aestivum L. في مؤشرات النمو الخضري والزهري والكيميائي والانتاجي.ص | An experiment was conducted in a field belonging to the Technical Institute - Kufa north west of Najaf governorate during the season of the year (2012 - 2013) to determine the effect of irrigation water quality and intensity of magnetically treated to three cultivars of bread wheat (Triticum aestivum L.) on vegetative growth, flowering, chemical and productive indicators.The experiment was designed randomized complete blocks (RCBD) arrangement Split split - plots and three replicatesfor each treatment. Experiment included study three factors is the quality of irrigation water (river water or well water) - Main plot, wheat bread varieties (IPAA - 99, Rashid and Tamuze - 2) - Sub - plot and magnetic treatment to irrigation water four intensities is (0, 750, 1500 and 3000) Gauss - Sub - sub - plot. The Revised Least Significant Difference (RLSD) at 0.05 probability was used to compare treatment means, whenever treatments affects were evident.Results showed : 1 - Wheat plants irrigated with river water were superior in all parameters in forms of plant height, leaves number, leaf area, tillers number; productive and non - productive, root length, lateral root length of the plant, leaves content from total chlorophyll, dry weights for plant vegetative, phosphorus% in grains, grains weight of spike, spike number of plant and m2, weight of 1000 grain, plant yield of grains, m2 yield of grains, hectare yield of grains per hectare compare to plants irrigated with well water, which recorded the highest chemical content of grains from nitrogen, potassium, magnesium, protein and carbohydrates percentages. The percentage of flowering, spikes length, spikes grains number were superior in the beginning with the well water, were superior at the end with river water.2 - Magnetic treatment with irrigation water by 1500 gauss revealed that noticeable superior was obtained in most of plants vegetative and floral characteristics, while the chemical characteristics had increased with magnetic water treatment by 3000 gauss and yield characteristics treated with 750 gauss. The effect of magnetic water treatment on leaves content from total chlorophyll and magnesium percentage in grains was negative.3 - The majority of vegetative, chemical and productivity parameters was increased with Rashid plants cultivar compared to same parameters for Tamuze - 2 plants cultivar which superior than IPAA - 99 plants cultivar except number of leaves, tillers number and spike grains number which superior with IPAA - 99 plants cultivar.4 - The triple interaction between experiment all factors revealed a significant effect on magnetized river water at 1500 gauss in Rashid and Tamuz - 2 plants cultivars giving highest vegetative growth indicators average which increased on plant yield with low magnetic water treatment (750 gauss) to Rashid plants cultivars and medium strongly (1500 gauss) for Tamuz - 2 plants cultivars, while the characteristics of grains chemical content were superior with same plants cultivars but magnetized treatment well water at 3000 gauss.

التشخيص الجزيئي للفطر المحاري Pleuroutus ostreatus وفاعلية بعض المخلفات الصناعية في انتاجه وقابليته الخزنية Molecular Diagnosis of The Oyster Mushroom Pleuroutus Ostreatus And Effectiveness of Some Industrial Waste In Its Production And Its Ability Storage

اسم المؤلف: سولاف حامد تيموز
اسم المشرف: عبد الامير سمير سعدون
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تضمنت هذه الدراسة انتاج الفطر المحاري ostreatus Pleuroutus باستعمال اوساط زرعية محضرة من مخلفات صناعة محلية وما لها من اهميه من الناحية الاقتصادية كونه يعد عنصرا غذائيا مهما يحل محل البروتين الحيواني المتمثل باللحوم، وما لهذا الفطر من اهمية طبية وعلاجية ا | This study included the production of mushroom oyster Pleuroutus ostreatus by using agricultural media prepared from local industry and which has importance in the economic district it's consider important nutrition element instead of animal protein representive by meat. this fungus has importance of medical and therapeutic, the results showed existence of chemical substances concentrated in the fruiting bodies such as Galic acid and Statin and other substances. that have had an active role in reducing cholesterol in the blood as well as break the clot blood through the presence of certain types of enzymes that break down blood clotting, which may occur, the results showed when studying the mushrooms from the genetic diagnosis molecularly using the technique of the PCR to determine the type of isolation that gives the production of economically significant. Results showed the presence of gene hereditary called Osteareolysin who works with several aspects of the mushrooms which give the recipe contrast pathogenic fungi plant also this study included re - using some of the remnants of plant starch corn husks and Al - gluten and corn embryos. the remnants of Maysan sugar Factory of material Molasses and Molass Baggaz and the study of their role as carrying materials, central ferment and growth of the oyster mushroom during agricultural it on these remnants. The results of the chemical analysis of the remnants of the sugar indestrey in Maysan Molasses and Baggaz and waste plant starch in Al - hashemeyeh / Babylon Al - Baggaz and yellow corn straw showed that molasses substance was contain the amount of sugars which sometimes reach to 41%, that making it as a good base of food to mushroom. When added molasses at different levels 2, 4, 6% to the central media the study showed a positive relationship between the levels of molasses and the radial growth of the oyster mushroom. The concentrations 25, 50, 100% of the leaky innate showed ability Tdhadah high - fungal plant pathogen (Aspergillus niger, Aspergillus flavus, Fusarium solani, Stymphllium sp., Penicillium sp.) Antigonsim when studying the negative ability of the fungus. The results showed a significant increasing in the growth standards and gaining characteristics of the oyster mushroom in the mass production of fruiting bodies called the gaining total according to the wet weight and it was higher production on corn husks and pomace dates 761.89, 735.13 g /kg of media and concentration of molasses 6%. when measuring efficiency of the fungus to product the largest amount of fruiting bodies observed that the middle pea - corn husks and pomace dates gave a larger amount of the remaining media and a concentration of 6% 76.18, 73.51%, have been affected by the weights of pods fungal change is another factor which affected by rates of molasses. The results were closely between nutrition media but nevertheless observed superiority of Corn husks and Pomace dates on the rest of media (15.04, 13.77)by using 6% concentration of molasses. when calculate the lengths of the necks of the body fruiting because of the great importance in a statement recipes - gaining produced and influenced by user medai - aligned and concentrations of molasses, as there were differences moral at all concentrations of molasses, when calculating the percentage of dry matter increased significantly increase the concentration of molasses and all concentrations compared with a comparison of strew wheat, which amounted to 12.16% when adding 2% molasses observed the emergence of highly significant differences between treatments and compared with control treatment, which gave treatment of Pomace dates highest rates, followed by corn husks and corn embryo and Al - gluten and Baggaz 8.21, 9.00, 9.28, 9.29%, respectively. For the purpose of obtaining material efficient in maintaining the objects fruiting intact throughout the storage period tested preservatives (such as Sorbic acid and black pepper) and concentrations 40.20g / l of articles in a row leads to prolong longevity reservoir simulation of the objects fruiting and maintain the quality characteristics of the objects fruiting. The results showed that the addition of material to the feeder molass medai AGRO of oyster mushroom had been increased the proportion of protein in the fruiting bodies, either with respect to calculate the proportion of carbohydrates had been found that the percentage of carbohydrates are associated with a high positive correlation relationship between the concentration of molasses and the different transactions that were used in the experiment. The oils were represented less than what can be had reached attribute to 0%. Well conducted safety checks the health of the mushroom oyster and the results were that the fungus free of toxins by anatomy organic to members liver, kidney and intestines and by the standards of blood physiological mice tested. And the importance of this project calculated the economic viability and profits and revenue obtained from such a project.

مقارنة لتشخيص الخمج بالمقوسات الكوندية بين النساء المجهضات والولودات طبيعيا مع دراسة للتغيرات النسجية المتسببة عن الخمج Comparative Diagnosis of Toxoplasma Gondii Infection Among Aborted & Normally Delivered Women With Study of Histological Changes As A Result of Infection

اسم المؤلف: فراس محمد بشير عبد الكريم الخشاب
اسم المشرف: ابراهيم شعبان داوود صباح سعيد محمود
الموضوع العام: علوم الحياة
السنة: 2009
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة التي امتدت من بداية شهر ايار (2007) لغاية نهاية شهر اب (2008) للتحري عن نسبة الخمج بالطفيلي المسبب لداء المقوسات بين النساء المجهضات والولودات طبيعا في بعض مناطق مدينة الموصل والمراجعات لمستشفى الخنساء التعليمي للولادة والاطفال.شملت عي | This study was conducted during the period from May 2007 to August 2008 to investigate the rate of infection with toxoplasmosis among aborted and normally delivered women in Mosul City territories who attending AL - Khansa'a educating hospital.Out of 150 women under study, 50 were aborted while 100 were normally delivered. The employed tests were included latex agglutination test LATEX and Enzyme Linked Immunosorbent Assay (ELISA) with its two types (ELISA - IgM), (ELISA - IgG) which used as a high specific and sensitive test to differentiate between types of infection acute, chronic and congenital. Toxoplasmosis was diagnosed in 28(56%) out of 50 abortive placenta and in 57(57%) out of 100 non abortive placenta (normally delivered women), these samples of placenta were microscopically examined after homogenizing and staining by Gimsa and Leishman's stain The parasite development stages were also noticed including developing from the tachyzoites into the oocysts stage and the histopathological effects resulting from infection in the placenta in both aborted and normally delivered women which were represented by necrosis, vaculation and cells degeneration , inflammatory cells infiltration and the focal inflammatory reaction in the villi in addition to hemorrhage and accumulation of eosinophilic substances, the results also showed that 41 (82%) of aborted placenta and 18 (18%) of non abortive placenta (normal delivered women) were infected with Toxoplasmosis by using the laboratory Balb/c mice inoculation method while, some cases of inflammation were diagnosed in the brain, of the experimental infected mice, which were represented by the inflammatory cells infiltration and the prevailing of gliosis, tissue necrosis vaculation and cells degeneration.This study revealed that laboratory mice inoculation in the peritoneal cavity is a useful and efficient method in the detection of the parasite in infected placenta as compared with the microscopic examination of direct smears and examination of tissue sections from placenta.The present study showed an overall percentage of infection among aborted women was (92%) while (58%) showed an acute infection and (34%) chronic infection, in another hand the infection rate among normally delivered women was (70%), and it has been found the infection rate among acute and chronic cases were (41%), (20%) respectively, Its to be noted this is the first time in Mosul City for such a study conducted in this area concerning the congenital Toxoplasmosis in newborns with an infection rate (9%).In this study, sera were tested by Latex (LAT), and Enzyme Linked Immunosorbent Assay (ELISA). It was shown that IgG - ELISA is the most sensitive to diagnose the infection as compared to other tests.These study also concerned with the isolation and diagnosis of Toxoplasma gondii parasite from blood by inoculating the blood samples in laboratory Balb/C mice the rate of infection was (16%) in laboratory mice inoculated with blood collected from aborted women while the infection rate in laboratory mice inoculated by blood samples (mother and her neonatal cord blood) collected from normally delivered women appear in (12%) & (16%) respectively. The rate of occurrence of the specific antibodies against Toxoplasma gondii was identified, The highest rate was found for the type (IgG+ - IgM+), in both aborted and normally delivered women with percentage reach (40%) & (26%). The titer (100 IU/ml) of the specific IgM & IgG antibodies was found in high rate reached (75%), (43.5%) in mother blood, also found in a high percentage in the neonatal cord blood estimated as (50%), (64.4%) for the specific IgM & IgG antibodies respectively, in another hand the titer (100 IU/ml) was also found to be the more predominate titer in blood samples of aborted women with percentage reached to (50%) & (60.5%) for the specific IgM & IgG antibodies respectively.

تاثير المدعمات المختلفة في حاصل ومكونات الفطر الغذائي Agaricus bisporus واختبار كفاءته في عدد من المتغيرات الكيموحيوية والفسلجية في اناث الجرذان Effect of Various Supplementation in The Yield And Compositions of An Edible Mushroom Agaricus Bisporus And Evaluation of Its Efficiency in Some Biochemical And Physiological Parameters in Female Rats

اسم المؤلف: جمال جليل احمد البياتي
اسم المشرف: كركز محمد ثلج الجبوري عبد الله عبد الكريم حسن
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: اجريت الدراسة في كل من مزرعة العرهون التابعة لقسم وقاية النبات - كلية الزراعة ومختبرات قسم علوم الحياة - كلية التربية للعلوم الصرفة في جامعة تكريت خلال المدة 1/9/2013 - 10/6/2014 تضمنت دراستين الاولى تم فيها تقدير تاثير اضافات متنوعة من المدعمات الكاربو | The study was conducted in each of the Al - Mushroom farm in Department of plant protection, College of Agriculture and the laboratories of Biology Department - College of Education for Pure Science at the University of Tikrit for a duration of 1/9/2013 to June 2014. The first studies were included the adding of carbon, nitrogen and other additives at casing period on productivity, quality characteristics and chemical composition of Agaricus bisporus mushroom. The results showed significant increased (p<0.05) in total production and biological efficiency when adding carbon supplement at 1% of starch and became 26.41 kg/m2 and 88.03% respectively, also with nitrogen supplement at 1% from Erbil feed (EF) that’s became at 23.20 kg/m2 and 77.33% respectively, compared with the same value in control group which at 21.68 kg/m2 and 72.26% respectively. Also both treatments were recorded earlier in production at 15 and 16 days after casing compared with 18 days in a control, while the qualitative characterizes has been the treatment of 3% from methionine the highest content of total antioxidants and became at 1975 µg/g dry weight compared to the 1572 µg/g dry weight in the control treatment, and the highest protein content was became at 40.32% in treatment of 1% of LB compared with 22.36% of their content in the control treatment. The treatment of 1% of EF was recorder as higher content from mineral of calcium, magnesium and iron at 390, 2574, 50.74 µg/g dry weight compared to the content in the control group at 290, 1683 and 37.64 µg/g respectively, and the treatment of sodium selenite 5% was recordes a higher content of selenium at 5.00 µg/g compared to the control treatment which at 4.00 µg/g dry weight. The treatment of EF also recorded a higher copper content at 108.60 µg/g dry weight compared to the control treatment which at 37.00 µg/g. The other study was apply to assessment the effects of replacing casein at 5, 10 and 15% by the Agaricus bisporus product from the treatments with starch and multi - vitamin and EF on some growth and physiological parameters in female rats feding for 28 days. The results showed that the presence of mushroom in food has caused a significant decline in weight. The present of mushroom powder at 5% in rats feed lead to significant increasing in hemoglobin and total account of RBC resulting in 12.53 and 6.36× 106 cell/mm3, 13.13 and 7.06× 106 cell/mm3, 7.36× 106 cell/mm3 for starch, multivitamins and EF, respectively, compared to 12.25 and 6.8× 106 cell/mm3, respectively. As well as the significant increased in the total counts of white blood cells that were at 5.50, 5.20 and 5.00 x 103 cells/mm3, respectively, with the starch, and at 5.70, 5.40 and 5.20 x 103 cells/mm3, respectively, with multivitamins and at 5.90, 5.50 and 5.40 x 103 cells/mm3, respectively, for the EF group compared with the control group were at 5.15 x 103 cell/mm3. also was significant increased in differential WBCs as lymphocytes with increasing the replacing percentage for treatments above. Also due the replace of casein by mushroom on proportions referred to in the above change in blood biochemicals parameters which significant decreased in urea, total cholesterol, triglycerides and low density lipoproteins and increase the concentration of high density lipoproteins, as well as reduced the activity of enzymes Alanine amino transferase (AST), Aspartic amino transferase (ALT) and Alkaline phosphatase (ALP) with increasing the percentage of replacing. As for the effect of replacing the same proportions of the mushroom instead of casein on growth and physiological characteristics of female rats with experimental diabetes induced by Alloxan and fed for 17 days. The results showed that the development of diabetes trial led to a reduction in the rate of animal weights and an increase in the relative weight of internal organs such as the heart, liver, spleen, pancreas and kidneys of infected sugar group, and became at 0.46, 4.39, 0.60, 0.46 and 1.19 g/100 g respectively when compared with the control animals, at 4.07, 0.42, 0.39, 0.23 and 1.04 g/100 g, respectively. And decreased of red blood cells that were at 5.93 x 106 cells/mm3, respectively, for the group affected by diabetes, while at 7.36 x 106 cells/mm3 respectively the healthy control group. And decreased WBCs in the infected animals with diabetes trial and became at 4.83 x 106 cells/mm3 compared with the healthy control group which at 7.03 x 103 cells/mm3. Also there was caused in significant increased in glucose concentration, which was on the first, 7th, 14th, and 17th days at 428, 405, 431.60 and 432.20 mg/100 ml respectively, in comparison with a control without diabetis that was at 123.33, 124.66, 126.33 and 125.33 mg/100 ml, respectively. Further to increased concentrations of urea, cholesterol, triglycerides and low density lipoproteins and a decrease in the values of high density lipoproteins also, increased the values of enzymes activity of each Alanine amino transferase(ALT), Aspartic amino transferase (AST) and Alkaline phosphatase (ALP) compared with the non - diabetic infected control group. That feeding female rats of experimental diabetes on the replacement rates of the mushroom instead of casein in their diet has led to the significant decline of the weight and relative weight of internal organs such as the heart, liver, spleen, pancreas and kidneys compared with animals infected with the induced diabetes with increasing rates of substitution. The results also showed that increased in total counts of red and white blood cells that was at 6.40 x 106 cell/mm3 and 5.00 x 103 cell/mm3 respectively when replacing 5% casein from mushroom compared to the infected control group were at 5.93 x 106 cell/mm3 and 4.83 x 103 cell/mm3 respectively. The substitution of casein with 5, 10 and 15% from mushroom was caused to significantly lower of glucose values to became 273.40, 251.60 and 302 mg/100 ml compared with control group infected which at 432.20 mg/100 ml. Also, because of the found of mushroom in the food was caused a changed in the biochemical blood, which significantly reduced the concentration of urea, total cholesterol, triglycerides, LDL and increase the concentration of HDL. As well as significantly reduced the enzymes (AST), (ALT) and (ALP) activity with the replacement rate increase compared with values in the animals infected with diabetes induced by Alloxan. The results concluded that the possibilty of use starch and EF at 1% for the commercial mushroom production. Mushroom also can be used as a food for lowering blood glucose, cholesterol, triglyseride, LDL and liver enzymes with increase in HDL for the people suffered from diabetes, obesity and heart diseases.

التاثيرات الفسلجية والكيموحيوية لعدد من المستخلصات النباتية في الدم والجهاز التناسلي الذكري في الجرذان البيض Rattus norvegicus المعرضة للكرب التاكسدي A Study of Physiological And Biochemical Effects of Plant Extracts on Blood And Male Reproductive System of Albino Male Rats (Rattus Norvegicus) Exposed To Oxidative Stress

اسم المؤلف: صاحب جمعة عبد الرحمن
اسم المشرف: زيد محمد مبارك المهداوي صالح محمد رحيم العبيدي
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الحيوان
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية معرفة بعض التاثيرات الفسلجية والكيموحيوية للمستخلصات المائية للكرفس (Apium graveolensL) والجرجير(Eruca sativa Mill) والطماطم (Lycopersicon esculentum Mill) في الدم والجهاز التناسلي لذكور الجرذان البيض المعرضة للاجهاد التاكسدي المستحد | The aim of the present study was to investigate some physiological and biochemical effects of the aqueous extract of celery (Apium graveolens L.), gardeen rocket (Eruca sativa Mill) and tomato frait (Lycopersicon esculentum Mill) on blood and male reproductive so system of Albino rats (Rattus norvegicus) exposed to oxidative stress induced by hydrogen peroxide 0.5% with drinking water for 21 days using vitamin E, as well known antioxidant as criterion for comparison. For the purpose of this study, preliminary experiments were carried out to find the most effective doses of plant extracts and it was found that these doses were 100, 150, 250 mg/kg body weight for, tomato, celery and gordeen roket extracts respectively. Fifty rats (12 - 14 weeks age) were randomly divided to ten groups (treatments) each with five replicates.These groups are : Group (1) (control group) : Rats without any treatment.Group (2) : Rats treated with H2O2.Group (3) : Rats treated with celery extract (150 mg/kg b.w.).Group (4) : Rats treated with celery extract (150 mg/kg b.w.) and H2O2.Group (5) : Rats treated with Eruca extract (250 mg/kg b.w.).Group (6) : Rats treated with Eruca extract (250 mg/kg b.w.) and H2O2.Group (7) : Rats treated with tomato extract (100 mg/kg b.w.)Group (8) : Rats treated with tomato extract (100 mg/kg b.w.) and H2O2.Group (9) : Rats treated with vitamin E (500 mg/kg b.w.).Group (10) : Rats treated with vitamin E (500 mg/kg b.w.) and H2O2 Results could be summarized as follow : 1. Compared with the control, the oxidative stress induced by H2O2 caused significant increase in total count of leukocytes, neutrophilis, acidophils, basophilis, monocytes and lymphocytes, and in the level of blood sugar (glucose), Total cholesterol, triglycerides(TG), low density lipoprotein (LDL - C), very low density lipoprotein (VLDL - C) atherogenic index (First and second), Urea, Malondialdehyde (MDA) and percentages of dead sperms and abnormal sperms.This stress showed as well, a significant decrease in body weight, Hb, PCV, high density lipoprotein (HDL - C), total protein, Albumin, glutathione, sperm total number, percentages of motile and normal sperms, testes weight, prostate and epididymis weights and seminiferous tubules diameter. This treatments, however, showed no significant variations in activity levels of ALT and AST enzymes and seminal vesicle weight.2. In comparison with treatment (2) (rats treated only with H2O2) , the treatment of rats, under oxidative stress with celery extract showed a significant decrease in total count of leukocytes neutrophils, lymphocytes and in blood suger, cholesterol TG atherogenic (First index) blood urea, MDA, Percentage of dead and abnormal sperms and seminal vesicle weight.This treatment showed also a significant increase in level of Hb, PCV., body weight, total protein, Albumin glutathione, number of sperm, percentages of motile and normal sperms, testis weight, epididymis weight and prostate weight. However no significant variations were detected in levels of acidophils, basophils, monocytes, HDL - C, atherogenic (second index) and seminituferous tubules diameter.3. Treatment of Eruca extract and compared with the treatment of H2O2, showed significant decrease in lymphocytes number and in the level of blood sugar, cholesterol (T.G), LDL - C, VLDL - C and atherogenic (first and second index) blood urea, MDA, percentage of dead and abnormal sperms and seminal vesicles weight. The treatment showed also a significant increase in body weight, numbers of acidophils, monocytes, basophils, HDL total sperm numbers, percentages of motil and normal sperms and weights of testis, epididymis and prostate.However, no significant variations were observed in Hb, PCV, Leukocyte total counts, neutrophil, albumin, ATL and AST and seminiferous tubule diameter.4. The treatment of tomato extract showed similar results to that of celary and Eruca extracts except, a significant increase in AST enzyme and seminiferous tubules diameter was detected in case of tomato extract (i.e not seen in case of other plant extracts).5. Compare with H2O2 treatment, rats under oxidative stress treated with vitamin E (500 mg/kg b.w) showed significant decrease in lymphocyte numbers, Total cholesterol, TG, LDL - C, VLDL - C, atherogenic (first and second index), urea, MDA and percentages of dead and abnormal sperms.At the same time the vitamin E treatment showed a significant increase in body weight , Hb, neutrophils, acidophils , monocytes, total protein, glutathione, total sperms number, percentage of motile and normal sperms, and weights of testis, epididymis, and prostate.However, this treatment (compared with H2O2 treatment) showed no significant difference in the level of PCV total count of leukocytes, basophils, blood sugar, HDL - C, albumin, ALT, AST, seminal vesicle weight and seminiferous tubules diameter 6. The most important histological changes exhibited by the animals under oxidative stress were apparent interstitial degeneration, necrosis of supporting and spermagonial cells, separation of these cells in semicyclic manner from the basement membrane, decrease in spermatids (spermatoblasts) and mature sperms in seminiferrous tubule cavity.These effects (due to H2O2 treatment) were decreased to minimal levels when the H2O2 treated rats exposed to plant extracts and vitamin E, used in this study.7. Animals treated with plant extract or vitamin E alone (i.e not treated before with H2O2) showed no clear difference from the control animal (without any treatment) though some improvement was observed in some physiological, biochemical and histological characters of blood and male reproductive system of the studied animals The above results revealed that the plant extract used in this study play an important role in decreasing the harmful effect of the free radicals in the animals (rats) studied.

دراسة جزيئية ومناعية لطفيلي اللشمانيا الجلدية Cutaneous Leishmania في المحافظات الوسطى والجنوبية Molecular And Immunological Study of Cutaneous Leishmania In The Middle And Southern Provinces

اسم المؤلف: ازهار موسى جعفر الموسوي
اسم المشرف: علي حسين الكبيسي مهدي حسين العمار
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: علوم الحياة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:
المستخلص: The study includes distribution of the Cutaneous Leishmania in some governorates in Iraq. Specimens are included cases of outpatients in hospitals like : Al - Karamah, Al - Sadir Teaching Hospital in Baghdad, Al - Hilla Teaching Hospital, Al - Hussein Teaching Hospital and Ein Altamer General Hospitals in Kerbala, Al - Sadir Teaching Hospital in Al - Najaf, Al - Karamah and Al - Zahraa Teaching Hospitals in Wasit, Al - Diwanyia General Hospital. Al - Hussein General Hospital in Al - Nasiriya, Al - Smawa General Hospital, Al - Sadir Teaching Hospital and Al - Qurna General Hospital in Al - Basrah during October 1122 to march 1121 Cutaneous Leishmania is consider a major problem that faces the patients because it causes deformities in the infection region. The study includes 330 cases of skin ulcer in which 225 of them were microscopically positive. Parasitic growth was done using two types of cultural media NNN and RPMI - 1640.The results have shown a considerable difference between male and female in which male was significantly exceeding 131) %22.11 (and female was 94) %72.44 (and 139) %72.44 (of outpatients from rural areas increased in numbers from outpatients of urban areas, and only) %12.11(from urban areas.The number of ulcerations in body has exceeded infection, that means more than one ulceration in male 83 (85%) and for female 41 (62%), and concerning infection in three or four ulceration, it is approximately equal and normally distributed on face areas 99 and a percentage of (44%) then lower arms are 92 and a percentage of (40.80%) and the upper parts are 33 and a percentage of (14.66%). Also, one infection was recorded on The study shows that 46 patients in a percentage of (%20.44) having a dry infection; whereas 179 patients (%79.55) having the wet type.For accurate diagnosis of Leishmaniaa species distributed in Iraq, PCR Was used technique and diagnosed two types of Leishmania parasite that causes the illness. A band in a length of 560 bp in 186 samples related to Leishmania major and the band sample was 750 bp related to 39 Leishmania tropica.Through studying the samples in Kerbala City, the number infected samples were 125 for the years of 2010 - 2011 the actuality is (73) infection and (52) infection from 2011 - 2012 in a ratio of (58.4 % and % 72.7 accordingly) in different inhibited areas in the governorate in which (51)) %71.2 (was in Ein Al - Tamir Suburb, and 29 cases of) %11.1 (in Al - Husseinya Suburb, as well as 17 cases of (13.6%) in Al - Hur Suburb, 16 cases) %21.2 (in the south quarters and 7 cases of (5.6%) in the north quarters and only 5 cases of (4%) in the city center.The molecular diagnosis in Polymerase Chain Reaction for Kerbala governorate samples have shown 31) %17.2 (of L.tropica, 22) %24.7(of them were male and 9 of) %4.1 (of them were female. L. major was 94 of) %42.1 (in which 55 of) %77 (were in male and 39 of) %12.1 (were in female.The immunological study for the patients serums with L. major by ELISA technique has significantly shown raised values of IgG and IgM during infection in comparison with the control group, then it declines slowly after treatment in which IgG was in ratio (1811.1 ± 523.1 mg/dl), and IgM was in ratio (166.7 ±23.6 mg/dl). Also, in L. tropica - IgG was in ratio (1722.1 ± 524.0mg/dl) and IgM was in ratio (25.3±182.9mg/dl) in comparison with control specimens, and then the ratios have declined after treatment dosages with Pentostam drug.The cellular dynamics values have shown an abstract increase in which interferon - gamma (IFN - ?) in infected patients with cutaneous L. major were (221.1±2.2mg/dl) which declined after treatment abstractly to (7.12±1.1mg/dl) as well as for L tropica (22.1±7.2mg/dl) which shows no significant abstract difference after treatment with control.An increase appears in cytokine IL - 10 that reached in L.major (122.1±8.2mg/dl) and after treatment was (8.11±2.2mg/dl) and in L. tropica was (222.1±2.2mg/dl) with no significant abstract difference after treatment with control specimen.As a simple attempt to find a vaccine for Leishmania disease a Lipophosphoglycan was isolated and purified as known factor for promastigot stage. The vaccine injected for two groups of Bulb mice in a concentration of purified vaccine (L.majorand Ltropica) to study the immunological response by detecting the Lymphocyte Transformation assay, the Delayed Type Hypersensitivity test and the PhagocytosisIndex.In lymphocyte transformation the ratio of L. tropica was % 7.2 and for L. major was % 21.7 and there is significant difference by P? 0.05 in comparison with control specimen in which its ratio was %7.4, and for Delayed Hypersensitivity test study the average foot thickness injected with vaccine and the other foot which injected with phenol - saline solution - only (1.11 ± 2.12ml) for the first group and (1.21 ± 2.72) for the second group, and for the control group it has registered (1.77± 1.15 ml) with a considerable difference of P? 0.05. By calculating the ratio of Phagocytosis Index cells, it was 17% and 27.6% for the first and second groups while for the control group it has been reaching 9.10%. Therefore, we can get advantage from these values as indicators for the rule of vaccine in the immunological response and triggering of vaccine idea for this disease.

البعد الجمالي في التكوينات الابداعية للخط العربي Aesthetic Dimension In Linear Formations Creative Calligraphy

اسم المؤلف: كريم سالم الساعدي
اسم المشرف: جواد عبد الكاظم فرحان الزيدي
الموضوع العام: الفنون الجميلة
السنة: 2014
الموضوع الدقيق: الخط والزخرفة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تعد مسالة الابداع والابتكار واحدة من اهم المسائل التي تشغل الانسانية في شتى مجالات المعارف والعلوم والفنون، فالابداع سمة العصر الحديث والطريق نحو النمو والرقي الحضاري، ولا يستثنى اي حقل معرفي او جمالي من الانشغال في قضية الابداع والابتكار فهما وسيلة التا | The issue of creativity and innovation is one of the most important issues of concern to humanity in various fields, Creativity characteristic of the times and the road towards progress and prosperity.To Aisttny any field of cognitive or aesthetic preoccupation with this issue, they are the means of meditation deep problems in order to develop solutions to the original and suitable.In Arabic calligraphy, which still indicates many of the questions and give a lot of fields of research is the study of dimensions of creativity is very important to deepen in the region to focus on the aesthetic and artistic objectives.When not studied this issue extensively, has directed the researcher to study through the four seasons, wondering in his first season for dimensional aesthetic that is available by formations linear, and attributes its transformation into a formality creative new leaves the context of traditional and maintains the rules discreet Arabic calligraphy.In the theoretical framework have addressed researcher in three sections to the concept of linear creative formations, as well as the characteristics of the formal and aesthetic and semantic configurable linear, and the effectiveness of Marja receive text language as a springboard aesthetic creative founder of the degree zero aesthetic in modern linear configuration.This included the third chapter action research to analyze the models selected sample and the approval of the orientations of the search, the according to a set of pillars appropriate to achieve the objectives of the research, and seal search a bid for the results showed the contribution of infrastructure design and processing of the contents of the text, and overlapping techniques races other technical in the incorporation of the dimensions of the aesthetic and creative in the configuration linear , as demonstrated by the conclusions of the possibility of achieving the objectives set for the search through the system prepared by the researcher and down to develop a set of recommendations and proposals.

الانفتاح العلامي في الفن المفاهيمي The Symbolic Openness In The Conceptual Art

اسم المؤلف: سامرة فاضل محمد علي
اسم المشرف: مكي عمران راجي الخفاجي
الموضوع العام: الفنون الجميلة
السنة: 2017
الموضوع الدقيق: الفنون التشكيلية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: عني البحث الحالي بدراسة ( الانفتاح العلامي في الفن المفاهيمي ) ويقع في اربعة فصول، خصص الفصل الاول لبيان مشكلة البحث واهميته والحاجة اليه، وهدفه، وحدوده، وتحديد اهم المصطلحات الواردة فيه. وقد تركزت مشكلة البحث على الطبيعة التاويلية للعلامة في ال | This research paper is interested in studying (the symbolic openness in the conceptual art) and consists of four chapters. The first chapter is about the research problem, importance, goal, limits and identifying the most important expressions. The research problem is focused on the interpretation nature of a symbol in the conceptual art in all its expressions resulted by the changes of the world after modernity including the social, politic, economic, and cultural pressures which affect negatively on the level of vision, acceptance and sense. This is guaranteed due to the relation between the art and the modern development which include all human durations across history to be in cope with this change. So the artist is obliged to be more courageous and spontaneous in expressing himself and thoughts to cross the limited lines for the traditional painting to present conceptual artistic works that are not restricted with one artistic style or type to include all exist in nature including the human body, language and consumable items. So the current research's problem is begun through answering the following question, (how can the artist make the recipient as a second author for the artistic script?).The importance of the research is clear because it establishes a critical study to give the searchers, and those who interest in the graphic sides, a chance to be informed about the kinds of the conceptual art including the symbolic openness.The searcher finds this study is so important because there are no previous studies focusing on the symbolic openness in the conceptual art. The searcher also wants to be interested in the basis of this study to fulfill the intended goal which is identifying the symbolic openness in the conceptual art.The subjective research limits include the study of the symbolic openness in the conceptual art in all its branches, elements, time limits (1965 - 2016) and place limits including (USA, UK, Germany, Mexico and France). The important expressions in this research are also clarified.The second chapter is about the theoretical frame and the previous studies which includes four topics : the first topic is about the theories of a symbol in the critical studies. The second is about studying the thoughtful basis of the symbolic openness in arts after modernity. The third is about the conceptual art, the conceptual context and the graphic system. The last is about the indicators. The second chapter ends with the previous studies which are not closed to the current study.The third chapter is about the research procedures including identifying all the samples which is (200 artistic works) and choosing and analyzing only (20 artistic works) intentionally.The fourth chapter includes the results which are : 1 - Achieving the symbolic openness through interaction and conversation and be away from autism and introversion in all research samples.2 - There are many sides of the conceptual art including the language, graphic art, geography and the human body. So it is an art of moved concepts which are effective in the daily life of a human being and his environment.3 - The idea in the conceptual art is the tool which makes the art through its philosophic embodiment. This leads to make an argument which raises questions that make the visual perceiver be in harmony with senses and mental action and the artistic work to obtain the symbols, signs and concepts. Another results are : 1 - The searcher tries to clarify the script origin and its openness to be interpretable to make the recipient (second author) read and understand more meanings and facts as in the research samples. 2 - The recipient is responsible for understanding the artistic work to create new items that help in understanding the work.The research ends with recommendations, suggestions, references and appendixes.

المثاقفة وتمظهراتها في العرض المسرحي العراقي المعاصر Acculturation And Its Demonstrations In The Iraqi Contemporary Theatrical Performance

اسم المؤلف: علي عبد الحسين عبد الشيباني
اسم المشرف: علي محمد هادي الربيعي
الموضوع العام: الفنون الجميلة
السنة: 2016
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: وظفت مفاهيم المثاقفة واجراءاتها العملية في ميادين انسانية كثيرة ومنها الفن المسرحي بوصف المسرح ميدانا لخطاب فني / انساني قائم على عمليات التواصل والتفاعل والتاثر والتاثير بين الامم والثقافات, وبين الذات والاخر على اساس من الشراكة الضمنية, يهدف الى انت | Hired acculturation concepts and procedures of the operation in humanitarian fields and many of them theatrical art as Theatre Square speech technician / inhumane based on communication and interaction, vulnerability and influence among nations and cultures, processes, and between the self and the other on the basis of the implicit partnership, aimed at the production of localized knowledge depends on the existence of an intellectual and artistic elements , went in my intention (ego) to (other). Where they can not conceive of a theatrical presentation does not belong in the process of becoming the Performing to acculturation with other operations, which carries the same deliberate motives, in order to influence the other heading his theatrical discourse, this study aims to monitor the acculturation concepts and mechanisms of functioning as it presents are manifestations which acculturation Which shows in multiple letters of knowledge (historical, literary and artistic), including the theater, especially in the theater, to open doors on jurisprudence and the experiences of the cultural dialogue and the exchange of knowledge - based and interactive communicative stems from acculturation as a truly civilized based on the interaction and communication between different cultures, which means that the play is based on acculturation play with the other is determined mainly through the presence of the tributaries of the play from different cultures, which requires the presence of acculturation theatrical haunted obsession identity and try to consolidate the theatrical phenomenon in the Iraqi theater and the Arab world.This dilemma stems from a researcher at the establishment placed his research is marked (acculturation and their appearance in the contemporary theater of Iraq). The study came in four chapters formulated according to the following conformation : Chapter One : a methodological framework for the study. The researcher of the research problem, related to the phenomenon of acculturation as a given, civilizational, cultural, and scientific and technical give would represent entirely on the nature of the performance and its practical applications in the theater. And for which I got transformations and alterations drastic manner display through the possibility of dialogue and interaction on the intellectual level and formal appearances and their interaction in the aesthetic and semantic markup.Then go researcher to the importance of research, and its objectives, and its borders, determine the terminology, where linguistic definitions and terminology adopted by the terms (acculturation) and (manifestations) depending on the operational definition for both.The second chapter, a theoretical framework, it came with three topics : First topic : Epistemology acculturation. Three axes.The first axis : Prospects and theoretical concepts.The second axis : the types of acculturation (levels - Varieties - mechanisms).The third axis : acculturation under cultural globalization.The second topic : data models of acculturation in a speech literary culture. Specifically in models including : (Trips - Translation - Intersexuality - comparison - Literary criticism).The third topic : theatrical manifestations of acculturation between local and global privacy. It came with double.The first axis : manifestations of acculturation in the world stage.The second axis : manifestations of acculturation in the identity and privacy of rooting in the Arab and Iraqi theater.He concluded the second quarter indicators of the theoretical framework and previous studies, and the most prominent indicators : 1. characterized acculturation theatrical feature correlation theatrical performances, all displayed offset by another offer, within the fabric of quotations and previous assignments and contemporary performances of different directions.2. benefit acculturation theatrical cultural exchange effect between culture and other humanitarian consideration given common heritage of all nations. The third chapter, research procedures, related to an inventory of community search and selection of research sample. Access to research sample that consisted of theater the following analysis : • A play women in the war.• A play Ali Al - ordeiy and rivals.• A play Camp.• A play red carpet. The fourth chapter, it has dealt with the results of the research sample and discuss the analysis, including : - Contributed acculturation operations in most of the presentations to the activation of the intellectual and cultural aspects on the one hand, and self - knowledge and point of reference on the other. - Dealing with cultural globalization as an imported culture, it was a positive feature, making them Meet director with the local culture to be intellectually tunic / new practice, as a show of renewal carries attributes.And then conclusions, and including : - The play with the offsets Cultural lead to the widening of intellectual meaning and significance, and not necessarily lead to the break approved expectation and create an aesthetic distance. Which establishes a mission to an area of interpretation. - The effectiveness of acculturation contribute to the theater to achieve a state of communication between what refers to the width of multiculturalism on the one hand, and between the cultural repertoire receiver on the other. Deepening receiving effective. Then came the recommendations and proposals that emphasized the importance of the effectiveness of acculturation in the play and the need to expand work out and study them systematically. Then concluded Find a list of references in Arabic and translated sources, foreign sources, as well as a summary of the search in English

جماليات الخطاب النسوي في فنون ما بعد الحداثة The Aesthetics Of Feminist Discourse In The Arts Of Postmodernism

اسم المؤلف: فرح علي عبد الرزاق الدليمي
اسم المشرف: محمد علي علوان عباس القره غولي
الموضوع العام: الفنون الجميلة
السنة: 2017
الموضوع الدقيق: الفنون التشكيلية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: عني هذا البحث بدراسة ( جماليات الخطاب النسوي في فنون ما بعد الحداثة) وهو يقع في اربعة فصول الاول لبيان مشكلة البحث، واهميته، والحاجة اليه، وهدفه، وتحديد اهم المصطلحات الواردة فيه، وقد انطوت مشكلة البحث الحالي على الكشف على ان ما شهده العالم الغربي من | Focus this research to study ( The aesthetics of the annual speech in the arts of postmodernism ) is situated in the four chapters of the statement of the research problem, and the importance of, and need for, and purpose, and to identify the most important terms contained therein, have involved the problem of current research to reveal that what the western world has witnessed the a change in the concepts and transformations of intellectual and what happened from the rapid changes has had a clear impact in bringing about changes in the various structures and as a result of those big changes that affected the cultural, literary and cash by engaging in systems as well as cultural, literary and artistic on the other hand engage in systems, it promotes the emergence of new types of literature including feminist who was an anti - hegemony of art and literature male has adopted the theses of feminist criticism pivotal link antithesis between accomplished male dominance and the accomplished feminist and a weak presence on an area of research literature, and the theses postmodern fertile area from which the feminist movement demands, general women and actresses in particular I have proven that every woman is able to achieve the same in the works of art and literature and in line with the philosophical and intellectual propositions of postmodernism.Hence the problem of current research originated through the following questions : - What is the role played by data feminist discourse in the art of post - modernism? - How did the contemporary visual artists, may file accomplishing visually crystallizes feminist speech from an aesthetic perspective holds insights and theses culture feminism? The importance of searching through explanation for literary and monetary concepts contemporary have a close relationship with art feminist masculinity and femininity, and the feminine and feminist and women's notions of parental (Patriarchate) and gender and its relation to the ideas of postmodernism, and represents an attempt to shed light on the nature of the relationship between the aesthetics of feminist discourse and the products of post - modernism, according to interested professionals through access to the findings and conclusions, it has found a researcher that there is an urgent need for this study is the fact that the subject has not been studied previously within the area effective for the products of arts postmodernism, as well as this study the concentration of matter on the contemporary visual artists and knowledge her methods performing and her creative achievements which has been absent for several decades.The aim of this research to know the aesthetics of feminist discourse in the arts of postmodernism was limited research on how long the time period of (1951 - 2016) and of studying the feminist productions for artists postmodern and executed with different materials at different ores in streams (abstract expressionism, pop art, visual art , graffiti art, conceptual art, art synthesis, new expressionism, digital art).The second chapter (theoretical framework) guarantees the three sections, first topic focus to the concept of post - modernism and the arguments of Netasheh and Freud, structural and some concepts that diffusion structuralism, deconstruction and the proposals of Jacques Derrida and Michel Foucault, and a speech after the buzzards and globalization, and a letter of postmodernism and the culture of consumption, while focus second topic entrance into feminism and feminist literature and criticism feminist, language and given the female of the text, while the third section rotation arts postmodern and implications of feminist discourse in streams (abstract expressionism, pop art, visual art, graffiti art, conceptual art, art synthesis new expressionism, digital art).Chapter three has included action research, the researcher reviewed the research community and appointed by the style selected.The fourth chapter focus on results search results and conclusions, summarized the researcher to a range of outcomes, including : 1. Feminist discourse depends on the products of postmodernism on data aesthetic transformation and stylistic and technical as well as formulations structural forms of intellectual and contents and their contents, which appeared in the second half of the twentieth century, has shown the emergence of post - modernism, according to the visions of intellectual feminist adopt a new attitude to express contemporary problems of women, as in the research sample models.2. Gaining visual artists experiments in post - modernism, the dimensions of functioning merge with the experiences of contemporary plastic artists, but up to focus on the necessities of research on the technical trend on the one hand the aesthetic and the inevitability of a departure from the prevailing traditional patterns on the other hand, as in the research sample models.3. The theme of alienation in the products of human visual artists, the investment pattern overlap between the conscious and unconscious, and between reason and emotion, a choice of intellectual touches the essence of the idea of self and carry meanings and connotations, as in most of the research sample models.The researcher has reached a number of conclusions, including : 1. Aesthetics feminist discourse in the arts contributed postmodern elevating of philosophy initiated no mind and the adoption of individual categories and chance and blow up the idea of art through the presence of the idea of demolition and dismantling of the bonds aesthetic realities.2. Uniformity intellectual formulations for aesthetics feminist discourse in the arts of postmodern philosophies with frameworks postmodern theses and feminist criticism, as well as other contemporary monetary concepts that have had an active role in deepening the critical reading and calendar visual feminist done.3. Interpret the visual landscape of the products of feminist art, aesthetically, as manifestation Layout hermeneutical reading and analysis, especially where infrastructure is enhanced mental scene assignments of symbolic probe form actually modulated semantic from the signifier and the signified. The researcher considers a series of recommendations including : 1. Interest in academic studies that specializes in visual themes of feminist art and that the lack of the existing ones in the scientific field, the big and important in the definition of this art with data and types.2. The establishment of cultural institutions and the houses of the Arab local publishing translated foreign sources dealing with plastic feminist art experiences in the world, the lack of such sources in specialized scientific libraries.3. The establishment of plastic arts exhibitions and annual festivals and events and periodic conferences, dealing with plastic feminist art, and the definition of the methods and insights, methods and propositions own visual artists.The researcher proposed the following headings study : 1. Aesthetics feminist discourse in modern art.2. Feminist discourse and representations in products of contemporary Arab art.3. The aesthetics of the image in contemporary feminist art
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