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تاثير التغير الاجتماعي في بناء الشخصية العراقية : دراسة سوسيو - انثروبولوجية في مدينة الناصرية نموذجا == The Social Change Effects In The Structure of The Iraqi Personality A Socio - Anthropological : Study In The City of Nasiriya As A Paradigm

اسم المؤلف: كمال رزاق حسين
اسم المشرف: صلاح كاظم جابر الصالحي
الموضوع العام: علم الاجتماع
السنة: 2013
الموضوع الدقيق: علم الاجتماع
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: ان دراسة تاثير التغير الاجتماعي في بناء الشخصية العراقية تمثل اهمية قصوى في علم الاجتماع والانثروبولوجيا نظرا لما يحدث من تغيرات في جوانب الحياة الاجتماعية, وتاثير هذا التغير في بنية الشخصية العراقية. فالشخصية تمثل احد اهم المحاور في المناقشات السياسية | In all human societies when social and cultural change accrues in one aspect of the social life the other aspects will influence in this change. That will extend to the social life of the individual and groups so as to their social personalities style. Th
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مشكلات العمل الصناعي من وجهة نظر العمال : دراسة اجتماعية ميدانية في مصفى الديوانية == The Industrial Labors Problems By View Point of Laborers Field Social Study At Dorah Refinery

اسم المؤلف: حيدر علي كاظم الرماحي
اسم المشرف: بسمة رحمن عودة الصباح
الموضوع العام: علم الاجتماع
السنة: 2013
الموضوع الدقيق: علم الاجتماع
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: First : the problem of the study : The problem with the study of ((the problems of industrial action from the viewpoint of workers)) that the problems which faced worker industrial different from the problems faced by the farmer or Earner in the agricul
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الذات والاخر في شعر عبد الوهاب البياتي == Self And The Other In The Poetry Of Abdul Wahab Al - Bayati

اسم المؤلف: رشا عبد الحسن عبد العظيم
اسم المشرف: هيام عبد زيد عطية
الموضوع العام: اللغة العربية وادابها
السنة: 2013
الموضوع الدقيق: الادب
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: Not without poem that presents itself scout for itself and visions from the others so desires nilly and keeps hair Iraqi ample room induces cases self - consciousness poetry and see the other, which is a mosaic is intertwined with the details and Taataalq movement filled openness and isolation at the same time, so it received placedself and the other great interest in psychological studies, and literary.... And under consideration to them as part of Aetjze of this integration and integration can not be aware of the individual itself only through knowledge of the other to him as a mirror of the self Therefore , any rupture or separation from the other works to break down the ego and threatened loss and marginalization losing its place among the community. Word when Bayati is part of the same charge the semantics of certain signals and gestures, he wanted Bayati to express his feelings toward the other of which became self - mergers with other one permeate to inside to see his thoughts and behaviors found vary sometimes differ at other times to open up possibilities for different cultural which have an impact onachieve a distinct form of hair.

الخطاب النقدي عند محمد حسين الاعرجي

اسم المؤلف: هيام عواد راشد
اسم المشرف: رحمن غركان عبادي
الموضوع العام: اللغة العربية وادابها
السنة: 2013
الموضوع الدقيق: الادب
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:

بنية الحديث النبوي : دراسة بنيوية == The Structure of Holly Prophet Hadhith : Structural Study

اسم المؤلف: عبد المحسن جاسم محمد
اسم المشرف: سرحان جفات سلمان
الموضوع العام: اللغة العربية وادابها
السنة: 2013
الموضوع الدقيق: الادب
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: This study is seeking to detect the structure of the Hadith, in light of the mechanisms proposed by the studies of structural which is aimed at the treatment of the text treatment dewy, recognize is form without Sultan directs, detailed from within, not o

اثر كتاب سيبويه في كتاب الحجة للقراء السبعة ابي علي الفارسي (ت377هـ) == Impact Sibawayh In Book Seven Argument For Readers Abu Ali Persian

اسم المؤلف: علي مطشر حاوي
اسم المشرف: جواد كاظم عناد
الموضوع العام: اللغة العربية وادابها
السنة: 2013
الموضوع الدقيق: اللغة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: Praise be to Allah, the Lord of the Worlds, and peace and blessings be upon our Prophet Muhammad and the God of divine. Mean and focused this study (after Sibawayh in the book argument for readers seven) Bektaben great for worlds sketching two writers to

البديع في علم العربية لابن الاثير (ت606هـ) : دراسة لغوية == Rhetoric of Linguistics By Ibn Al - Atheer (D. 606AH) : Linguistic Study

اسم المؤلف: نبا عبد الامير عبد الحميداوي
اسم المشرف: حيدر حبيب حمزة الجبوري
الموضوع العام: اللغة العربية وادابها
السنة: 2013
الموضوع الدقيق: اللغة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:

عبد الله النديم دوره السياسي والفكري حتى عام 1881م

اسم المؤلف: اسيل مهدي شاهر الحميداوي
اسم المشرف: محمد صالح حنيور الزيادي
الموضوع العام: التاريخ
السنة: 2013
الموضوع الدقيق: التاريخ الحديث والمعاصر
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
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خالد العظم سيرته ودوره في السياسة السورية 1903 - 1965 == Khaled Al Azem's Biography in Syrian Politic 1903 - 1965

اسم المؤلف: وائل عدنان محمد الحسيني
اسم المشرف: حسن علي عبد الله السماك
الموضوع العام: التاريخ
السنة: 2013
الموضوع الدقيق: التاريخ الحديث والمعاصر
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
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التحليل المكاني لخصائص السكان النشطين اقتصاديا في محافظة كربلاء المقدسة للمدة (1997 - 2011) == The Spatial Analysis of Characteristics of Economically active Population in Holy Karbala Governorate (1997 - 2011)

اسم المؤلف: وسيم عبد الواحد رضا النافعي
اسم المشرف: حمادي عباس حمادي الشبري
الموضوع العام: الجغرافية
السنة: 2013
الموضوع الدقيق: الجغرافية الاقتصادية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:

تقويم عملية الخصخصة في بلدان عربية مختارة مع اشارة خاصة الى العراق == RECTIFYING THE PRIVATIZATION IN SELECTED ARAB CONTRIES WITH THE CASE IN IRAQ

اسم المؤلف: فاطمة مصحب لفتة
اسم المشرف: موسى خلف عواد
الموضوع العام: الادارة والاقتصاد
السنة: 2013
الموضوع الدقيق: الاقتصاد
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:

واقع التنمية المكانية في محافظة القادسية وافاقها المستقبلية

اسم المؤلف: سحر كريم كاطع علي الناشي
اسم المشرف: كريم سالم حسين الغالبي
الموضوع العام: الادارة والاقتصاد
السنة: 2013
الموضوع الدقيق: الاقتصاد
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:

التغيرات الفسلجية لضربين من الباميا Abelmoschus esculentus (L.) Moench بتاثير حامض الدبال والزولفاست == Physiological Changes To Varieties of Okra Abelmoschus Esculentus (L.) Moench By Effect of Humic Acid And Zolfast

اسم المؤلف: ندى سالم عزيز الموسوي
اسم المشرف: عبد الامير علي ياسين
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: نفذت هذه الدراسة في كلية الزراعة/ جامعة القادسية خلال المدة من 10/4/2011 حتى 15/10/2011. الهدف منها دراسة الاستجابة لضربين من الباميا (الحسيناوية والبتيرة) لطريقتي اضافة حامض الدبال ومستحضر الزولفاست (الرش الورقي ومع ماء الري) وبتركيزين لكل منهما (الموصى | This study was conducted in Agriculture College, AL - Qadisiya University during the period of 10/4/2011 till 15/10/2011. The aim of the study was studying response of two okra varieties (Al - Husenawyia and Al - Pteira) humic acid and zolfast application methods (foliar and addition to irrigation water) and this concentration (recommended and doubled) on growth and productivity as well as leaf mineral and fruit medicinal active constituents.The humic acid and zolfast formulas were added by the use of hand sprayer or with irrigation water with the recommended and doubled concentrations for humic acid and zolfast as foliar spraying were 1 ml.L - 1 and 2 ml.L - 1 if doubled respectively while when used with irrigation water 4.8 ml.L - 1 and 9.6 ml.L - 1 if double for humic acid 7.2 and 14.4 ml.L - 1 for zolfast if doubled.The design of the experiment was Randomized complete blocks in a factorial arrangement (2×2×5) in three replications. The RLSD at 0.05 was used as a method to compare means separation treatment effect was evident. The results showed : 1 - Al - Husenawyia variety was superior in total leaf area, vegetative fresh and dry weights, root fresh and dry weights, P% and content addition to specific leaf weight, relative growth rate, fruit length, number of fruit/plant, fruits yield compared to Al - Pteira. Percentage of protein, carbohydrates, total soluble solids, caprylic, lauric saturated fatty acids and all the unsaturated fatty acids. Mucilage, folic acid and oil refractive index were also higher with Al - Husenawyia compared to Al - Pteira had higher stem diameter, fruit fresh and dry weight, K%, boron content and percentage of saturated fatty acids (Myristic, Palmatic, Stearic, Arachidic and Behinic). Ca% was the same for both cultivars.2 - Foliar application method of humic acid and zolfast showed beneficial effect on and all parameters studies except root fresh and dry weight and percentage of saturated fatty acids increased with irrigation water (except Lauric acid which was increased with foliar application). Methods of application did not affect stem diameter, Ca% in leaves and fruit total soluble solids.3 - Although the double recommended concentrations of humic acid and Zolfast reduced saturated fatty acids significantly their recommended concentration lowered saturated fatty acids (Caprylic, Myristic, Palmatic, Stearic, Arachidic and Behinic) compared to control treatment. Percentage of fiber was lower with the use of zolfast compared to humic acid and control. While K% was lowered when zolfast used in the double recommended concentration. No significant difference were found between the recommended concentrations of humic acid or zolfast in stem diameter, total leaf area, relative growth rate, vegetative dry weight, root dry weight, P%, Ca%, boron content, fruit length, number of fruit/ plant, fruit dry weight, mucilage, carbohydrates%, folic acid, oil%, percentage of saturated fatty acids (Caprylic, Myristic, Palmatic and Arachidic) and unsaturated fatty acids (Oleic and Linoleic) and the percentage of unsaturated fatty acids/saturated.4 - The interaction between cultivars and methods of application revealed that foliar application of both humic acid and zolfast reduced saturated fatty acid (Caprylic, Myristic, Palmatic, Stearic and Arachidic) significantly of both cultivars, compared to their addition to irrigation.5 - The interaction between cultivars and substances of application (humic acid and zolfast concentrations revealed that both cultivars had different response in some traits of studied to applied concentrations of humic and zolfast. Al - Husenawyia variety had higher vegetative fresh weight, root dry weight and fruit of mucilage content with the double recommended humic acid. While Al - Pteira variety had higher TSS% and Lauric acid% when used the double recommended concentration of zolfast.6 - The interaction between methods of application and concentrations of application substances showed superior effect on some studied parameters. Foliar application method was superior on addition to irrigation application with all concentrations except root fresh and dry weight and saturated fatty acids% (except Lauric acid) which were increased when added to the irrigation water to all concentrations of application substances. The same interaction did not revealed significant difference in stem diameter, Ca% and fruit fresh and dry weight.7 - The three way interaction factors studied in the experiment revealed an increase in growth parameter of both cultivars when humic acid and zolfast were added in their higher concentration (double recommended) by foliar application expect root fresh and dry weight and saturated fatty acids% (except Lauric acid) which were increased when added to the irrigation water. Al - Husenawyia variety was higher in all parameters with the use of higher concentration of humic acid and zolfast when applied with irrigation water or foliar application. The three way interaction revealed no significant difference in stem diameter, vegetative dry weight, Ca%, fruit fresh and dry weight, TSS%, percentage of oil and unsaturated fatty acids (Linoleic and Myristoleic).8 - Results of electrophoresis analysis revealed that the use of the doubled recommended concentration of humic acid and zolfast with irrigation water participated in building higher protein molecules in fruits to both cultivars. While the double recommended concentration of humic acid and zolfast did not show this trait when foliar applied.

انتشار داء اللشمانية الجلدية في محافظة القادسية مع تقييم مدى الاسجابة للعلاج بعقار البنتوستام بطريقة RT - PCR == Prevalence of Cutaneous Leishmaniasis In Al - Qadissia Province And The Evaluation of Treatment Response By Pentostam With Rt - Pcr

اسم المؤلف: رنا صالح صاحب محلول الدفاعي
اسم المشرف: غيداء عباس جاسم الجبوري
الموضوع العام: علوم الحياة
السنة: 2013
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: Results of clinical and laboratory examination of 239 cases showed 55 infection cases in Cutaneous Leishmaniasis and in infection proportion reaches at 23.01% out of the total number of the Cutaneous cases which are similar to Leishmaniasis during the months of the study. The Results also showed that the highest infection was recorded between the ages 1 - 10 years old and about 26 cases in 47.27% and between 10 - 20 years old about 17 cases in 30.9% and the lowerest was between 40 - 50 and 70 - 80 years old in one cases in 1.8%. The summit of the disease spread was in February for about 24 cases in 43.63% specially in AL - Hamza AL - Sharqi where the highest number of infection was recorded for about 23 cases in 41.81%. It is followed by AL - Shafiya for 10 cases in 18.18% and the lowerest was AL - Mhannawiya for one case in 1.8%. It has been clear that the number of the multiple epidemics is more than the individual ones in 70.5% while the latter in 29.98% respectively. It was focused on hands and legs in 50.35% and 24.46 %. The tissue test showed thick perspiration in the big infected pharynx and lymphatic cell in addition to the existence of abscess to the smash of the infected cell. Forty nine positive cases were found in 89.09% for the genetic material DNA which is connected with Leishmaniasis by mean of NestedPCR to identify the type results showed 47 samples which is L. major in 95.91% and to get sure by investigating the virulence factor lipophosphoglycan, proteophosphoglycan and cysteine protease it was found that all sample were positive in 100% as Leishmania. major. To know the affect of treatment pentostam on these samples, the standard proportion for the genetic patterns was calculated factor lipophosphoglycan, proteophosphoglycan and cysteine protease during five week. Results showed that there was a big difference for each gene in a solation during the period of treatment in a level of the possibility of (P?0.05) when used test LSD. and by comparing the three genes it was found that there was a meaningful between cysteine protease and the other two factor lipophosphoglycan, proteophosphoglycan and there wasn’t difference between lipophosphoglycan, proteophosphoglycan. Hence, it has been clear that the treatment pentostam has a big affect in parasite by its affect on the virulence factors and this explains that possibility of infection may happen again after having the treatment. The skin disease cutaneous Leishmaniasis is considered as one of the endemic parasitic diseases in Iraq. This study aims at identifying its prevalence and specifying the virulence factors in Cutaneous Leishmaniasis in order to define the type of disease reason and the affect of the treatment of Pentostam on the virulence factors lipophosphoglycan, Cysteine protease, proteophosphoglycans by means Real time - PCR

دراسة الاصابات الفطرية الجلدية لدى الاطفال في موضع الحفاظة == Mycological Infections In Children In The Position of The Diaper

اسم المؤلف: عباس عبد الحسين محي
اسم المشرف: ماجد كاظم عبود الشبلي
الموضوع العام: علوم الحياة
السنة: 2013
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:

تاثير اشعة الفا على التصاقية بعض انواع البكتريا == Study The Effect of The Alpha Ray On The Adhesion Some Type of Bacteria

اسم المؤلف: فردوس غضبان كاظم
اسم المشرف: زياد متعب الخزاعي
الموضوع العام: علوم الحياة
السنة: 2013
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية جمع 37 مسحة من المرضى المصابين باخماج العيون في مدينة الديوانية ومن كلا الجنسين حيث تضمنت الدراسة سيادة نوعين من المسببات البكتيرية لاصابات العيون ودراسة قابلية التصاق هذه البكتريا على العدسات اللاصقة وهي المكورات العنقوديـــــــــــ | The current study included (37) samples were collected from sicks of Eyes infection in Al - Diwaniya city from two kinds that the study included two type dominant of Bacteria causes for Eyes infection and study this Bacteria adhesion ability on the Lenses, that the Staphylococcus aureus and Pseudomonas aeruginosa as well as the impact of exposure to (Radiology Alpha and Gamma rays) on adhesion ability, As well as the search included the application of a mathematical model to adhesion formula. The current study results are showed the Staphylococcus aureus for percent (45.94)% and then the Pseudomonas aeruginosa for percent (32.43)%. The results were obtained being clear impact of irradiation on Bacteria adhesion ability to Lenses. The results were obtained the higher level adhesion reaching its, the Staphylococcus aureus in the natural case to (cfu/mm2 10 - 6×292) that in three hours whereas the Alpha ray exposure more than adhesion level that range six hours to (cfu/mm2 10 - 6×302) and when the Gamma ray exposure the higher adhesion level reaching that range (cfu/mm2 10 - 6×233) during exposure period that reach to four and half hour. About the Pseudomonas aeruginosa the adhesion higher level in the natural case to (cfu/mm2 10 - 6×204) during two hours and half hour whereas when exposure its, to Alpha ray that reaching to higher level adhesion range (cfu/mm2 10 - 6×298) during six hours as well as when exposure to Gamma ray the higher level adhesion reaching range (cfu/mm2 10 - 6×250) during four and half hour. Application the mathematical adhesion sample, the result obtained that the disengagement coefficient, the valuable impact its, so that the valuable increased as well as temporal to be late in approach stage of zero who mathematically certain the lenses importance that the important dangerous effective for Eye infection distribution. As well as the results were certain the Alpha and Gamma rays were irradiation, that have impact of where decreasing the adhesion bacteria numbers for lenses, that decreasing from Eyes infection

تاثير الاشعة فوق البنفسجية Ultraviolet (UV) على بعض المعايير التكاثرية (معامل الانقسام للحيامن والتشوهات في رؤوس النطف) في الجرذان البيض == Effect of Ultraviolet On Some Reproduction Parameters (Mitotic Index of Sperms And Head Sperms Aberration)

اسم المؤلف: قيس هادي لايذ الكرعاوي
اسم المشرف: حسين خضير الميالي
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: الحيوان - الفسلجة
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: قسمت 20 جرذ ابيض من الضرب Balb/C الى اربع مجاميع بعد تشعيعها (تشعيع الجسم كله) بفترات تعريض للاشعة فوق البنفسجية Ultraviolet لمدة (0 و20 و30 و60) دقيقة، ثم شرحت بعد 7 ايام من التعرض للاشعاع وتم حساب معامل انقسام mitotic index الخلايا الجنسية بالاضافة الى | Twenty white rats (Balb / C) have been divided into four groups after their exposure to ultraviolet ray for the following time periods (0, 20, 30, 60) minutes. Then the autopsy has been performed after 7 days of (UV) exposure and the mitotic index of sexual cell division has been counted. as well as the Head sperms aberration. The results of the study showed the following : 1 - Decrease in the mitotic index for the sexual cell. This decrease is directly proportional with the duration of exposure to radiation.2 - A slight increase in the percentage of the Head sperms aberration between (T 20) in comparison with the control group which is not exposed to radiation (T 0).3 - The increase in percentage of the Head sperms aberration is due to the (UV) and this abnormalities are directly proportional with the length of the exposure period as (T 30, T 60).

علاقة بعض الهرمونات والمعايير الكيموحيوية للمصابين بحصى المرارة == Relation of Some Hormones And Biochemical Variables For Patients With Gallstones

اسم المؤلف: انسام فيصل ياسر الجوراني
اسم المشرف: احسان ريسان ابراهيم الركابي
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: الحيوان - الفسلجة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: صممت الدراسة الحالية لمعرفة مستويات بعض الهرمونات والمتغيرات الكيموحيوية لممصابين حصى المرارة، اذ اجريت الدراسة على المرضى الوافدين الى مستشفى الزهراء التعليمي ومستشفى الكرامة التعليمي / قسم الجراحة في محافظة واسط للمدة منذ بداية شهر تشرين الاول (2012) حت | The study was Designed to investigate some hormonal levels and biochemical variables in patients with gallstones, where the study was conducted on patients attending Zahra Hospital and Karama Hospital / Department of Surgery in Wassit province for the period since the beginning of the month of October (2012) and until the end of the month of April (2013), The number of patients (75) were aged between 15 - 70 years and the number of males (15) and females (60) compared with healthy controls and numbered (25) aged between 15 - 70 years and the number of males (5) and females (20). Blood samples were collected in volume (10 ml) blood serum was isolated to achive required measurements, which included measuring the level of the hormone leptin, thyroxine (T4), thyroid stimulating hormone (TSH), growth hormone (GH), also the study included estimate the concentrations of biochemical parameters : total cholesterol (T.Chol), higher density lipoprotein cholesterol (H DL - Chol), factor risk (RF), liver enzymes ALT and AST, total serum bilirubin (TSB), glucose, creatinine, albumin, Globulin, and total serum protein (TSP), The results of the study were as follows : .Prevalence of gallstones in Wassit province, and Females injury rate will gallstone in wassit province much higher than males with rate of 5/1. Significant increase (P <0.05) in the levels of leptin hormone and GH as well as non significant increase (P >0.05) in the levels of the hormone TSH, while there was a significant decrease (P <0.05) in the level of the hormone T4 in people who suffer from a gallstones disease compared with healthy people. Significantly increase (P <0.05) in the concentration of T.Chol, and RF, while there a non - significant decrease (P> 0.05) in the concentration of HDL - Chol. significant increase (P <0.05) in the concentration of ALT, AST, TSB, and glucose with a non - significant increase (P>0.05) in the concentration of createnine. significant decrease (P <0.05) in the concentration of albumin, TSP, while an increase is not significant (P>0.05) in the concentration of globulin. There is effect of sex, age, BMI, and duration of the disease for patients with gallstones compared to healthy persons. There is effect of the taking contraceptive pills for female patients infected with gallstones compared to patients female non Taking contraceptive pills.

التعبير الجيني للمورث Erg11 وعلاقته بالمقاومة وظاهرة رجوع النمو لبعض عزلات خميرة المبيضات البيضاء المختبرة تجاه مضادات الازول الفطرية == Gene Expression of Erg11 And It’s Relation With Resistance And Trailing Growth Phenomena of Some Isolates of Candida Albicans Tested Against Azole Antifungals

اسم المؤلف: حيدر عبد الحسين عباس
اسم المشرف: عدنان حمد عبيد الحمداني
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: هدفت الدراسة الحالية الى الكشف عن دور المورث Erg11 والتعبير الجيني النسبي له في مقاومة خميرة المبيضات البيضاء C.albicans وعلاقته بنشوء عزلات مقاومة او ظاهرة رجوع النمو Trailing growth لهذه الخميرة عند اختبار حساسيتها الدوائية تجاه بعض مضادات الازول (الفلو | The current study aimed to detect the role of Erg11 gene and its relative expression in the resistance of Candida albicans, and its relationship with the emergence of resistace or Trailing growth isolates when tested its susceptibility against azoles agenst (fluconazole, ketoconazole). A total of 120 specimens were collected from patients of both gender with different ages whom infected with oral thrush, uro - genital and Gastrointestinal candidiasis and admitted the General Teaching Hospital and materinaty and children Teaching Hospital in AL - Diwaniyah city during the period from December/2012 to February / 2013. The isolation and identification of yeast isolates were done based on their morphological, Cultural and biochemical tests in addition to use the confirmatory systems such as the growth on Chrom Agar candida, Api Candida and Tobacco Agar medium to distinguish C.albicans from other yeast spp. Also, some virulence factors of C.albicans such germ tube formation, chlamydospores production, growth at of 45 c and resistance to cycloheximide were detected. The antibiotics sensitivity test was used in two method (disk diffusion & dilution) to determine the sensitive, trailing growth, resistance isolates and minimal inhibitory concentration (MICs) toward azole agents. The qRT - PCR was used to detect the relative quantitative of Erg11 gene expression and its relationship with resistance to azole and emergence of trailing growth phenomena in the presence of Fluconazoles and ketoconazole. The isolation and identification results revealed that the isolation percent of C.albicans was 47.05 % incomparison with other Candida species that were 15.3%, 11.76 %, 8.23 % and 5.9 % for C.krusi, C.dubliniensis, C.tropicalis, C. parapsilasis and C.glabrata , respectively. The antibiotic susceptibity tests of C.albicans showed the presence of resistance precent (38.89%) to fluconazole and the most of sensitive isolates revealed a trailing growth phenomena in the zone of growth inhibition of floconazole in a ratio 55.56%. While the resistance percent to ketoconazole was 27.78 % and the sensitive isolates showed the trailing growth in a ratio (38.89%). The statistical analysis showed a significant differences (P <0.05) among tested treatments. The MICs values of fluconazole against C.albicans isolates were 8, 16, and 64 Mg/ml for sensitive isolates and were 0.125 and 0.5 Mg /ml for resistance isolates and the trailing growth phenomena occurred at MICs value ? 8 Mg/ml within 24 hours of incubation while the values became ? 64 Mg/ml after 48 hours of incubation.There was a significant differences (p< 0.05) among tested treatments isolates incubated at 24 hours while there was no significant differences (P>0.05) at 48 hours of incubation. In respect with Ketoconazole, the resistance of C.albicans was increased after 48 hours of incubation at MICs values 0.125, 0.25, 0.5 and 1 Mg /ml while its was sensitive at values 4, 8 and 16 Mg /ml and the trailing growth phenomena occurred when the MICs ? 2 Mg/ml after 24 hrs of incubation, while it reached to ? 8 Mg/ml after 48 hrs of incubation. There was a significant differences (p< 0.05) among tested treatments incubated after 24 hours while there was no significant differences (P >0.05) among treatments after incubation 48 hours. The results of relative quantitative gene expression for both genes revealed a significant differences (p< 0.05) in gene expression levels through the occurrence of increased in the transcription of mRNA quantity among tested treatments.The up - regulation of Erg11 and fold change in mRNA transcription was 2.498 fold compared with 1.0 for control treatment after 24 hrs of incubation in the presence of antifungal, while the increasment was 12.606 fold after 48 hrs of incubation of isolates with antifungals.
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