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الكشف الجزيئي عن بكتريا Oxalobacter formigenes باستخدام جينات 16SrRNA، oxc في البراز من اشخاص اصحاء == Molecular Identification of Oxalobacter Formigenes Bacteria Based On 16S rRNA, Oxc Genes In Stool Samples Healthy Subjects

Author name: طارق طلال خلف
Supervisor name: زهرة محمود الخفاجي
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: بكتريا Oxalobacter formigenes من مكونات المكنون البشري الميكروبي، تتغذى على الاوكزالات السامة وبذا تخلص الجسم منها. البكتريا سالبة لصبغة كرام، ولا تكون الابواغ ولاهوائية المعيشة، ويمكن عزلها من الغائط البشري، ولدراسة البكتريا من النواحي الجزيئية، تم جمع ع | Oxalobacter formigenes is one of the human microbiome, uses the toxic oxalate for their growth (Oxalotroph), and helps the body to get rid of excess oxalate. The bacterium is Gram negative, non - spore former, obligate anaerobe, and can be isolated from human stool.To carry out molecular studies on this bacterium, 50 samples of human stool were collected from subjects with wide range of ages (3 - 63 years) and different genders, subjects were healthy and have no medications and especially antibiotics for at least for 3 months. Genomic DNA was extracted with special kit, to amplify certain housekeeping genes, these are 16S rRNA (3' end) segment having the anti - Shine - Dalgarno sequence (ASD), and the oxc gene responsible for production of oxalyl - CoA decarboxylase (middle region responsible for active site at where the Thiamin pyrophosphate binds, the enzyme co - factor). It was possible to amplify the 16S rRNA gene in 46 sample out of 50 (92%), and 7 samples out of 50 (14%) for oxc gene. Analysis and alignments of 16S rRNA sequences put the Iraqi samples in the O. formigenes i.e., the same species depending on Distance Score of alignments, it has been found that ASD of bacteria is conserved and represented by the sequence " 5' CGCGGUGAACGUUCCCGGG3' " in the studied samples and in strains found in the International Databases. Segment of oxc (~ 600 bp) translated into proteins (190 - 194 amino acids) were aligned with oxc protein of O. formigenes (M77128) reference strain, it has been found that this segment similar to the TPP binding site and characterized by its high hydrophobicity

المراتب التصنيفية للنباتات البرية من ذوات الفلقتين في منطقة صدور - ديالى == Wild Dicots Plant Taxa of Sudoor - Diyala Province

Author name: نسرين صبار هاشم حسين المهداوي
Supervisor name: علي حسين عيسى الموسوي | وسام مالك داود
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: This study examined the current survey of the vascular plants of dicotyledon wild during the study period (2013 - 2012) In the area of Sudoor - Diyala, the study was based on the 774 samples of plants with their duplicates as collected by The Researcher. All of these collections have been cacched in the Iraqi herbariums. To be specific there are 186 species which relate to 133 genus and 43 families. All these, have been scientifically classified with thier local and common Iraqi or arabic names.Besides, their duration and economic importance (medical, toxic, nutritional or forage, artificial, harmful, aromatic, ornamental, fuel plants or other uses). Moreover, their geographical distribution in the districts of Iraq and thier geographical existence in neighboring countries to Iraq (Saudi Arabia, Kuwait, Turkey, Iran, Syria and Jordan) , and the study results statistics have shown that the vast majority of the collected species are Herbal Plants totalling 164 species out of 179 species in all.This, in fact, can be ascribed to the impossibility of txonomizing 7 plants species for the incapacity to obtain their flowers or fruits. As for the shrub species it amounts to 13 species where as the number of woody species of trees is 2 only. As for the economic plants and their significance, let s mention the fact that the number of plants used as forage amounts to 86 species, the medical plants are 83 species, the toxic plants fall into 43 species, the ornamental plants are 23 species, the industrial plants are 14 species, the weed plants are 11 species, the aromatic plants are of 6 species, and the plants used as fuel are of 4 species. It also appears that the majority of the plant in the area of study is widespread in the various districts of Iraq, i.e. the number of such plants amounts to 110 species, however, there are plants of medium spread in the districts of Iraq, i.e. the number of such plants amountsto 54 species.There still are some plants whose geographic distribution has no been identified in the districts of Iraq and their number is 15 species, and as for their geographic extension into the neighboring Countries, these plants amount to 116 species in Saudi Arabia, 84 species in Turkey, 81 species in Iran, 79 species in Kuwait, 69 species in Syria and 26 species in Jordan. Furthermore, the species of plants collected perviously and have not been found during the period of study amount to 32 species and these relate to 13 families.The shrinkage of the vegetation cover in the area of Sudoor is attributed mainly to the drought conditions and the wind soil erosion which the area has undergone in the previous years. Besides, there are some other reasons including the overgrazing, the expansion of agriculture and the unstudied projects of construction, which have resulted in the disappearance of natural plants in the area.

دراسة المشعرة المهبلية Trichomonas vaginalis والجراثيم المرافقة لها في نساء مدينة تكريت == Study of Trichomonas Vaginalis And Bacteria Associated With It In Tikrit City'S Women

Author name: ميلاد عدنان مزهر
Supervisor name: حسين ساهر اسود العبيدي | عبد الله حسين عبد الله الجبوري
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تناولت الدراسة الحالية عزل طفيلي المشعرة المهبلية T. vaginalis والتعرف على انواع البكتريا المرافقة له من النساء اللواتي يعانين من التهاب المهبل Vaginatis المراجعات لمستشفى تكريت التعليمي قسم النسائية والتوليد للمدة مابين اب 2007 ولغاية نهاية شباط 2008،بلغ | The present study included the isolation of T. vaginalis and conducted to determine bacteria associated with this parasite in women attending gynecology and delivary department in Tikrit teaching hospital suffering from vaginitis from august 2007 till the end of February 2008. One hundred ninety three samples were collected, their ages ranged between (14 - 57) years.The rate of infection with this parasite was 9.3%. it was not associated with bacterial in a proportion 33.3%, where as 66.7% was associated with bacterial infection.Bacterial vaginitis represented 80.4%, S. aureus was the most commonly isolated associated with T. vaginalis. It was with a proportion 41.8% and was the most common bacteria represented 21.3% among other bacterial species causing vagintis. The sensitivity of diagnostic tests were variable since the direct examination was the highly sensituse test (100%) in comparison with staining using giemsa and gram stains, their sensitivity were 19.6% and 0.0% respectively. The most common clinical symptoms related to trichomoniasis infections were discharges with burning and itching which represented 44.8% and white discharge was 64.7% which represent discharge according to color.. According to epidemiology of this parasite, the highest percentage of infection was 14.6% in urban women with secondary educational level and with high socioeconomic level while represent 22.7% and 15.8% respectively and the highest rate of infection (12.9%) as recorded in ages group (24 - 33) years. In married women the proportion of infection was 9.4% while in women whom were not child bearing it was 15.9%. Also a highest infection rate 16.6% was recorded in women who using antifertility tablets. The present study also determined the relationship between the pH of vagina and types of infection since high infection rate was with parasite 91.7% and 97.4% of bacterial vaginatis at pH ? 4.5. The present study showed a highly significant elevation in E2 hormone valvo in pregnant and nonpregnant women infected with trichomoniasis while this hormone was decreased in sterile women infected with trichomoniasis and in pregnant and nonprengnant and sterile women infected with bacteria vaginatis.

تاثير نقص البروتين في بعض التغيرات النسجية والسريرية والوظيفية في ذكور الفئران البيض == The Effect of Protein Deficiency In Some Clinical, Histological And Physiological Changes In Male White Mice

Author name: نسم عماد دايم الفؤادي
Supervisor name: فاضل فرهود مكي الجبوري | هاشم محمد عبد الكريم العلاق
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: The present study is designed to know the effect of protein deficiency on mice males through study the clinical histological changes of some organs including (liver, kidney, stomach and testes).In addition of study the blood and biochemical parameters, where they were sheltering animals in the animal house of the branch of physiology / College of Veterinary Medicine / University of AL - Qadisiya and the number was 90, all of the mice are males and age (28 - 30) days kept for two weeks before starting the experiment for the purpose of acclimatization was given during this period, diet control, then the experiment began between 01/05/2014 until 19/2 / 2014, and at weights ranging from start racing experience between 17 - 18 g, all in a period of growth.These animals were divided into three groups, each group contains 30 mice, first group (control) was give 18% total protein contains animal protein 7%, while the second group was given 13% total protein contain 3.5% animal protein, and the third group was given 10 % total protein without any animal protein for different period as follow (15, 30, 45)day.Experiment takes study of changes in body weight of animals and some selected organs also study histological changes for studied organs, also physiological blood parameters such as PCV, Hb, GCS and TSP.Results showed significant decrease (p<0.05) in weight body rate of animals, also in the rate of selected organs means compared with control group. The second treatment for 45 days most significant in decreased of body weight and organs weight if it compared with first treatment in all periodsHistological procedures showed that clinical and histological changes are graduated by the severe of protein deficiency and period of time. The histology investigation for liver showed that congestion of blood vessels accompanied with dilation of central vein and portal vein, and necrosis of the hepatic cells and destroyed the general total structure of liver. The section of kidney showed that necrosis and destroyed in renal tubules and epithelial cells in the lumen of tubules and bleeding in renal tubules and clear shrinkage in glomerulus. There are histological changes in stomach by necrosis and desquamation for gastric cells in both period (30, 45) days, while there are no histological clinical changes in both doses in period (15 days) for exposure. Histological sections of testes showed induction inhibition of spermatogenesis and desquamation of germ cells and congestion in the interstitial tissue and dilation of lumen of seminiferous tubules in both periods (30, 45) days, while there is no any changes in both first and second treatment in period of (15)days.In contrast, the hematological parameters showed that the concentration of Hb, PCV significant decrease (p<0.05) in blood of treatment animals which correlated with protein deficiency compared with control group, and the second treatment for (45) days showed the most significant in the parameters compared with the first treatment and for all periods.Biochemical tests obtain significant decrease (p<0.05) in blood glucose level in both treatment first and second in first period. Also the results showed that no significant increase in first treatment in both periods (30, 45) days compared with control group, while there is significant increase in the level of blood glucose in the second treatment in both periods (30, 45) days compared with the control group. Also did not score a significant decrease (P <0.05) in the total serum protein in thefirst treatment in the first period (15) days, compared with the control group, and found a significant decrease (P <0.05) in the first treatment in the two periods (30, 45) days compared with the control group, while found a significant decrease (P <0.05) in the total serum protein in the second treatment period (15, 30, 45) days compared with the control group.It can be conclusion that the exposure for protein deficiency due to clear clinical histological changes for studied organs and some changes in some hematological and biochemical parameters in white mice which treated with protein deficiency which it causes these symptoms

تاثير المستخلص المائي الخام لنبات الزنجبيل Roscoe officinale Zingiber على بعض الجوانب الدموية والتناسلية في الفئران الحوامل == Effect of Crude Aqueous Extract of Zingiber Officinale Roscoe On Some Hematological And Reproductive Aspects In Pregnant Mice

Author name: رغد خالد موفق مسلم
Supervisor name: محمد عبد الهادي غالي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: اجريت هذه الدراسة للتعرف على مدى تاثير المستخلص المائي الخام للزنجبيل Zingiber officinale Roscoe (الطازج، اوالجاف) على الفئران الحوامل المجرعة عن طريق الفم وبالتراكيز (284، 568، 1136، 1420 ملغم/ كغم) اثناء مراحل الحمل المختلفة (المبكرة والوسطية والمتاخرة | This study was designed to investigate the effect of the crude aqueous extract of ginger (Zingiber officinale Roscoe) (fresh or dry) on pregnant mice orally administered at doses (284, 568, 1136, 1420 mg/kg) during different durations of pregnancy (early, intermediate, and final) for a period of 7 days for each duration. The study showed that there was no significant difference in the average weights of the animals at (P < 0.05) after treatment with (dry or fresh) extract at doses (284, 568, 1136 or 1420 mg/kg) comparing with the control group.The parameters of blood in the early duration of pregnancy showed a significant decrease in the average of hemoglobin (Hb) at (P < 0.05) after treatment with (fresh or dry) extract at the doses (568, 1136, 1420 mg/ kg), while the average of packed cell volume (PCV), demonstrated a significant decrease after treatment with fresh extract at doses (284, 568, 1136, 1420 mg/kg), and at (568, 1136, 1420 mg/kg) after treatment with dry extract at (P < 0.05) : The average of red blood cells (R.B.C.) after treatment with (fresh or dry) ginger extract, at (P < 0.05) showed a significant decrease at doses (284, 568, 1136, 1420 mg / kg), while the average mean corpuscular hemoglobin (MCH), and the average mean corpuscular volume (MCV) at (P < 0.05) revealed a significant increase and at doses (568, 1136, 1420 mg/kg) after treatment with dry extract, at doses (1136, 1420 mg/kg) for the average of (MCH), at doses (568, 1136, 1420 mg/kg) for the average (MCV) after treatment with fresh extract, while the average Mean corpuscular hemoglobin concentration (MCHC) did not show any significant difference at (P < 0.05) after treatment with (fresh or dry) extract comparing with the control group.The parameters of blood in the intermediate duration of pregnancy showed a significant decrease at (P < 0.05) in the average of (Hb), (PCV) and (R.B.C.) at doses (284, 568, 1136, 1420 mg/kg) after treatment with fresh extract, while dry extract demonstrated a significant decrease in average of (Hb) and (PCV) at doses (568, 1136, 1420 mg/kg), and a significant decrease in the average of (R.B.C.) but at doses (284, 568, 1136, 1420 mg/kg), while the average of (MCH) and (MCV) showed a significant increase at (P < 0.05) and at doses (568, 1136, 1420 mg/kg) after treatment with (fresh or dry) extract, while the average of (MCHC) did not showed any significant difference at (P < 0.05) after treatment with (fresh or dry) extract comparing with the control group.The parameters of blood in the final duration of pregnancy revealed a significant decrease (P < 0.05) in the average of (Hb), (PCV) and (R.B.C.) at doses (284, 568, 1136, 1420 mg/kg) after treatment with (fresh or dry) extract, while (MCH) showed a significant increase at (P < 0.05) and at doses (568, 1136, 1420 mg/kg) after treatment with (fresh or dry) extract, (MCHC) did not demonstrated any significant difference at the level (P < 0.05) after treatment with (fresh or dry) extract at all doses, while (MCV) showed a significant increase at (P < 0.05) and at doses (284, 568, 1136, 1420 mg/kg) after treatment with fresh extract, and at doses (568, 1136, 1420 mg/kg) after treatment with dry extract comparing with the control group. The results showed a significant decrease at (P < 0.05) in the average of diameters of ovarian and corpus luteum after treatment with (fresh or dry) extracts in the early and final durations of pregnancy at doses (568, 1136, 1420 mg/kg), and in the intermediate duration after treatment with (fresh or dry) extract but at doses (284, 568, 1136, 1420 mg/kg) comparing with group control.As for the concentration of the hormone estrogen (? - estradiol) didn’t demonstrated a significant difference at (P < 0.05) during different durations of pregnancy after treatment with (fresh or dry) extract at all doses, whereas progesterone showed a significant decreased at doses (284, 568, 1136, 1420 mg/kg) in all durations after treatment with (fresh or dry) extract comparing with the control group. Using doses at (284, 568, 1136, 1420 mg/kg) with (fresh or dry) extracts revealed a significant increase at (P < 0.05) in the average number of infected follicles in the all durations of pregnancy comparing with the control group. The results showed that there are no significant difference in the lengths of the embryos during intermediate and final durations at (P < 0.05) after treatment with (fresh or dry) extract at all doses comparing with the control group.There are also histopathological changes in the ovary, uterus and placenta included degeneration in ovarian follicles, necrosis, congestion, hemorrhage, edema, vasodilatation and rupture in blood vessels at different using doses.These findings indicated that the treatment with (dry and fresh) extract at doses (568, 1136 or 1420) mg/ kg harmful on pregnancy in different durations (early, intermediate, and final) , also the using of fresh ginger more harmful than dry ginger.

التحري عن جينات المقاومة لمضادات البيتالاكتام واسعة الطيف في بكتريا Acinetobacter baumannii المعزولة من مصادر سريرية == Detection of Extended Spectrum Beta - Lactam Resistance Genes In Acinetobacter Baumannii Isolated From Clinical Sources

Author name: صفا ماجد محمد الباجلاني
Supervisor name: عباس عبود فرحان الدليمي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: تضمنت الدراسة عزل وتشخيص 16عزلة من بكتريا Acinetobacter baumannii من اصل 196 عينة جمعت من مصادر سريرية مختلفة في مستشفى بعقوبة العام ومستشفى البتول التعليمي وكانت اعلى نسبة عزل لهذه البكتريا من مسحات الجروح %10.8، ومسحات الحروق بنسبة 8.3%، والادرار 6.9%، | The study included isolation and diagnosis of 16 isolates of Acinetobacter baumannii out of 196 samples collected from different clinical sources in Baquba General Hospital and Al - Batool Teaching Hospital. The highest rate for the isolation of these bacteria from wounds 10.8%, burns 8.3%, urine 6.9%, and blood 5%. The diagnosis of isolates was confirmed by ViTEK2 device in addition to phenotypic, microscopic characteristics and biochemical tests.The results of the investigation of virulence factors for Acinetobacter baumannii isolates showed that all isolates have the ability to adhesion surfaces of epithelial cells of humans (100%), while the ability of 13 isolates to produce biofilm was 81.2%, seven isolates had possessed efflux pumps with high efficiency (43.7%). The investigation of the sensitivity of the isolates for ten antibiotics, the results showed all isolates were resistant to antibiotic cephalexin was 100%, against antibiotics meropenem and imipenem was 50%, the other antibiotics resistance ratios ranged between 81.2% - 56.2%. The results of multi - drugs - resistance tests showed ten isolates of Acinetobacter baumannii possessed of multiple antibiotic resistance, the isolates were divided into two groups, resistance group and other sensitive depending on the resistance to antibiotics, as the first group included ten isolates resistance from 6 - 9 antibiotics, the second group included six isolates resisted from 1 - 4 antibiotics, and the first group is dominant in the study. The concentration (100 µg/ml) of cephalexin, cefotaxime, ceftriaxone to test the ability of isolates were resistant to cephalosporin antibiotics, all isolates showed resistance to this concentration for antibiotic Cephalexin, the resistance Cefotaxime, Ceftriaxone to this concentration was 75% for both antibiotics. The combination disk method used to investigate isolates producing for extended spectrum ? - lactamase enzyms and metallo ? - lactamase enzyms the results indicated that the percentage of isolates under study producing extended spectrum ? - lactamase enzymes and metallo ? - lactamase were 62.5% and 56.2% respectively from the total 16 isolation. It was determined the minimum inhibitory concentration MIC for cefotaxime and ceftazidime by method of multiplying serial concentrations, The results showed that the minimum inhibitory concentration for cefotaxime values ranging between 32 - 1024 µg/ml, as for the MIC ceftazidime his values ranging between 16 - 1024 µg/ml. Conducted process extract bacterial DNA for isolates of Acinetobacter baumannii, then held polymerize chain reaction PCR for isolates resistant to antibiotics ? - lactam and MIC values more than 64 µg/ml through the use of specialized primers that target specific sequence of genes blaCTX - m and blaSHV, relay outputs of the multiplication agarose gel concentration of 1%, the results showed that 5 isolates of Acinetobacter baumannii out of 10 isolates possessed gene blaCTX - m and by 50%, as for blaSHV gene the results showed that three of the isolates under study only has this gene and by 30%.

التاثيرات النسجية والدمية وبعض النواحي الوظيفية لعقار البيروكسيكام في ذكور الفئران البيض Mus musculus == Histological And Hematological Effects And Some Physiological Aspects of Piroxicam In Male Albino Mice Mus Musculus

Author name: بشرى عمر احمد معروف
Supervisor name: انتظار محمد مناتي
General topic: Biology
Specific topic: Zoology - Histology
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: The current study aimed to detect the effect of Piroxicam in making behavioral, hematological and physiological changes in the kidneys of adult male albino mice Mus musculus, The present study also included measuring the diameters of glomeruli, proximal and distal convoluted tubules as well as the histopathological study.A total number of (128) adult male albino mice were utilized in this study, they were randomly distributed into four main groups, the first three groups were orally treated with concentration (50, 100, 150) mg/kg respectively, the forth group considered as control group were orally treated with (0.1) ml of distilled water for eight weeks, the samples were collected every two weeks for the histological study, blood samples were withdrawn for the hematological and physiological studies.The current study showed that there were behavioral changes observed on the experimental animals, represented by reluctance to food intake, lethargy, tremor, introversion, fast breathing, change in eye’s color and blackened feces, and the intensity of these symptoms increases with the increment of concentration and administration period.The statistical study results revealed that there was significant decrease at (P<0.05) in the mean numbers of red blood corpuscles, hemoglobin concentration and the percentage of packed cells volume in orally treated groups with (50, 100, 150) mg/kg for (6, 8) weeks of administration in comparison with control group, as for the white blood cells the statistical results for this study showed significant increase (P<0.05) in their mean number in treated groups with concentration (100, 150) mg/kg for (6, 8) weeks of administration compared to the control, whereas the groups treated with concentration of (50) mg/kg showed no significant changes along the administration period.Also all the concentrations showed significant differences (P<0.05) in the mean concentration of urea, creatinine and potassium ion in the blood serum of orally treated mice at the last weeks of administration in comparison with the control group.The statistical results of the present study also revealed that there were significant differences (P<0.05) in the mean diameters of renal glomeruli of the treated groups with concentration (50, 100, 150) mg/kg for eight weeks while there were non - significant differences in other periods, also there was non - significant decrease in the mean diameters of proximal convoluted tubules in the treated groups with concentration of (50, 100, 150) mg/kg in all periods except the period four weeks it showed a significant decrease (P<0.05) in the mean diameters of proximal convoluted tubules of the groups treated with the concentration (100, 150) mg/kg in comparison with control group. The statistical results also clarified that there were significant differences (P<0.05) in the mean diameters of distal convoluted tubules of the treated groups with concentration (100, 150) mg/kg for six and eight weeks of administration, while the group treated with concentration (50) mg/kg had no significant differences over the whole administration period.The study also recorded the presence of histopathological symptoms in the kidneys of treated mice with the dose (50)mg/kg represented by hemorrhage, vascular congestion, calcium casts formation inside the lumens of renal tubules, while the groups treated with (100)mg/kg of the drug showed a fibrin deposition inside renal tubular lumens, infiltration of inflammatory cells, cytoplasmic vacuolation in the epithelial cells of the renal tubules, and sloughing epithelium of renal tubules, while in the medullary region, there were epithelial cells atrophy in the renal tubules. The histological examination of mice kidneys treated with (150) mg/kg showed the appearance of hyalinization, shrinkage and complete loss of glomeruli, Pyknosis of nucleus and fibrosis around the blood vessels as well as the presence of oedema which occupied a large area of the kidney tissue

تقييم نوعية المياة الصناعية ووحدات المعالجة لمعمل النسيج القطني في الكاظمية == Evaluation of The Industrial Wstewater And Treatment In Al - Kadhimia Cotton Weaving Industry

Author name: اسيل حسين محمد السعداوي
Supervisor name: سهير ازهر الكاظمي
General topic: Biology
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: This study includes two axis, the first one determined the physical and chemical characteristics for several concentrations of industrial wastewater of cotton textile factory, that located in Al - Kadhmiya, which are disposable to the main assembly in the treatment unit (first unit) of the factory ; Also the final water quality was evaluated after the treatment in the fourth unite and compared with normal environmental averages (or compared with acceptable limits for discharged.The second axis of this study assessed the efficiency of the physical and chemical treatments in the third unit, and also the biological treatments in the fourth unit through detecting the removal percentage for each stage of the treatment to evaluated the treatment unit efficiency of this factory. Samples were collected from four treatment units for six month, single sample was collected per two weeks.The (local, field) tests were assessed for the collected samples including (Temperature, PH, DO and EC, while the laboratory tests included TSS, TDS, BOD, COD, oil and grease, So4 - 2 and heavy metals such as Pb, Zn and Cr).The results showed that the temperature values were with (normal range) acceptable limits (22.3C?) in the first unit and (19.8C?) in the fourth unit; PH values also were within normal limits in the almost treatment units (neutral), however, the electrical conductivity average was 3123?c/cm in the first unit, and 1101 ?c/cm in the fourth unit ; Moreover, the average values of COD were 580 mg/L in the first unit and 163 mg/L in the fourth unit. The removal percentage was 41% in the primary sedimentation in thesecond unit while it was 36% in the third unit for biological treatment.The BOD values were 487 and 302 mg /L in the first and fourth unit respectively ; the removal rates were 27% and 29% for primary sedimentation stage and biological treatment respectively and these rates are acceptable for normal limits.In general, the final treatment concentration was 302 mg/L in the fourth unit which is normal environmental rang, and also BOD concentration was not exceeded the environmental limits in the final stage of the treatment while the total percentage dissolved salts were 37% and 22% for the third unit which are normal in comparison to the Iraqi limits after the treatment for the fourth unit (703mg/L). Furthermore, the results of suspended solids found that the removal percentage was 76% for the second unit and 43%for the third unit while the total concentration of the last treatment was 61mg/L which is with normal limits, also the concentration of lipids of oils and grease was 0.8 mg/L for the fourth unit, while the removal percentage was efficient for the primary sedimentation and biological treatment. The heavy metals concentraitions were now with water and the main assembly tank and this was within normal environmental limits.

دور اجهاد المضاد الحياتي (سيفتازديم) في امراضية الممرضة البولية الايشريكية القولونية المنتجة للبيتا لاكتاميز موسعة الطيف في الفئران المختبرية == Role of Antibiotic (Ceftazidim) Stress On Pathogenicity of Uropathogenic Extended Spectrum ? - Lactamaes E. Coli In Laboratory Mice

Author name: علي حسون حمادي عبد الله
Supervisor name: محمد قيس العاني | حارث جبار فهد
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: Arabic
University location: Anbar
First pages:
Abstract: جمع 60 نموذجا من عينات بول وسط المجرى من المرضى الراقدين في المستشفى الذين يعانون من اخماج المجاري البولية من مستشفى اليرموك ومستشفى الكرخ ومستشفى القديس رافائيل/ الراهبات للمدة من 27 شباط 2014 ولغاية 25 اذار 2014، وقد اظهرت نتائج الزرع البكتريولوجي الحصو | Sixty mid - stream urine specimens were collected from patients presented with urinary tract infections whom attending Al - Numan, Al - Yarmouk, Al - Karkh, and Saint Raphael/Al Rahibbat hospitals in Baghdad for the period February, 27th to March, 25th 2014. Bacteriological culture identified fifty (80.33%) isolates as E. coli depending on cultural and biochemical characteristics as well as Api and Vitek 2 automated systems. However, 82% of isolates developed ? - hemolysis on blood agar plates.Susceptibility of all isolates against seven commonly prescribed antibiotics were investigated and 96, 82, 84, 92, 92, and 56%. of isolates were resistant to ampicillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, cefalothin, imipenem, and meropenem, ceftazidime, respectively.Concomitantly, ? - lactamase production was detected phenotypically by disk replacement method. The results showed that 82% of all isolates have the ability to produce ? - lactamase. Interestingly, correlation coefficient between ? - lactamase and hemolysin production was 0.99.Using microtiter plate method, all E. coli isolates were tested for their ability to form biofilm. The results revealed that 36, 54, and 10% of isolates formed biofilm with variable thickness; weak, moderate, and strong, respectively.Alongside with previous findings, blaCTX - M - 2, blaVIM, blaTEM, and blaPER were identified in 18, 12, 10, and 4, respectively. Nevertheless, none of blaOXA - 1, blaOXA - 4, blaOXA - 30, blaIMP, blaCTX - M - 1, blaCTX - M - 9, blaKPC, and blaSHV. The isolate number 11 harboured the highest number of genes (blaTEM, blaVIM, and blaPER) among all tested isolates. Twelve albino mice was divided randomly into four groups comprising A through D injected with ceftazidime at sub MIC, E. coli 11, E. coli 11 with ceftazidime solution, and standard strain, respectively.Histopathological sections did not show any changes in respeuct to group A. however, group C suffered signs of infection less than those appeared in group B sections. Simultaneously, group D suffered intense histpathological changes more than other groups infected with resistant isolate.

دراسة علاقة سم Ochra. A بحالات الفشل الكلوي غير معروف الاسباب في محافظة القادسية == Study The Relationship of Ochra.A With Kidney Failure of Unknown Case In Al - Qadisiya Governorate

Author name: هدى رحيم هاشم الموسوي
Supervisor name: بهيجة عبيس حمود الخالدي
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Doctorate
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: اجريت هذه الدراسة لتحديد العلاقة بين السموم الفطرية وحالات الفشل الكلوي غير معروف الاسباب لدى المرضى المراجعون الى مستشفى الديوانية التعليمي للفترة من اذار/2012 الى كانون الثاني/2013 والذين تم تشخيصهم من قبل الاطباء المختصون. اظهرت نتائج فحص عينات الادرا | This study was designed to detection the Relationship between some fungi and it’s toxin with Renal falier in patients suffer from Renal falier attended to Al - Diwanyia teaching hospital in period from March/ 2012 to January/ 2013, These patients were diagnosed by physician in above hospital. By using Thin Layer Chromatography (TLC) the results showed 23.07% from patients blood specimens had Ochratoxin A, 19.23% from patients urine specimens had Ochratoxin A 38.46% from patients had Ochratoxin A in blood and urine where only 59.61% your blood and urine were clear from above toxin, So healthy persons were had Ochratoxin A in your blood and urine by rate 20% 40% respectively and 10% from them had Ochratoxin A in your blood and urine and only 65% from them don’t had Ochratoxin A in their blood and urine.the concentration of Ochratoxin A in blood were ranged between 6.2 - 25.8 ?g/kg from weight boody and 4 - 10.3?g/kg in urine and in healthy reach concentration of Ochratoxin A in blood 2.3 - 3 ?g/kg from weight boody and 4.3 - 4.6 ?g/kg in urine.The data of this study showed the male highly infected 87.5% with ocratoxin while in female 70% and 71 - 80 years age had highly infected 92.85% with ochratoxin A. The results of study of biochemical parameter in patients showed highly raised in WBC and urea and Keratinin to reach to 12000 corpuscle /ml3 310 mg/dl, 12.2 mg /dl respectively and lowed the activity of alkaline phosphates enzyme and albumin into 2.2 u/l, 1.5 gm/dl, respectively and suger 120 mg /dl. The results of isolation and diagnosis by depending on Morphological, Microscopical and Biochemical characters showed there were two species of fungi belong to Aspergillus sp, include A. ochraceaus 25.42%, A. niger 12.71% and A. candidus 15.25% and Candida albicans 46.61% were isolated from urine patients where only C. albicans and C. krusi were isolated from blood of patient by percentage 66.66% and 33.33% respectively. So the results of single PCR method selected to detection the OCR and PSK genes in Aspergills spp which responsible for ochratoxin A production , showed 83.33% from A. ochraceaus isolates had OCR gene (907 bp) and 66.66% from A. nger had PSK gene (584bp)Also the results of this study showed A. ochraceus and A. niger had ability to produce metabolic compounds when grow on yeast - extract broth and these compounds had significant toxic effects(P?0.05) in biochemical parameters in albino rats represented raised number of white blood cells (WBCs) into 10610 cell/µm3, urea into 228 mg/dl and Keratinin into 15 mg/dl in blood of animals which treated with 5000 ?l/kg from metabolic products of above fungi respectively, comparative with control groups 10100 cell/µm3. in other side, these metabolic products caused in same dose lead to lower of albumin and activity of alkaline phosphatase enzyme into 2.8 mg/dl and 3.7 u/l respectively where normal level for this parameter in control groups.Histological study showed the dose of metabolic products 500 - 5000 µl/kg for both fungi caused pathogenic changes in kidney represented by, atrophy highly proliferation of cells in endothelial layer of Bowman's capsules in addition to hemorrhage and Focal agrgregate inflammatory cells.In other side, study of toxicity of ochratoxin A which extracted by using thin layer chromatography (TLC) teqnique from urine and blood of patients and yeast extract broth media that had growth of A. ochraceus and A. niger showed signification effects(P?0.05) for this compound in physiological and biochemical parameters also caused significant effects in histological parameters similar to these effects which occurred in previsious experiments while these effect don’t appear in control groups.

تحديد القابلية التمنعية لمستضد الفوعة لبكتريا (Vi) Chitosan المحملة على حبيبات Salmonella typhi وذيفان الكزاز في الجرذان == Determination of Immunogenicity For Virulence Antigen of Salmonella Typhi Antigen Conjugated To Chitosan And Tetanus Toxoid In Rats

Author name: رغدة سعد محمد العمري
Supervisor name: زياد متعب الخزاعي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: This study was carried out during the period from October 2013 to September 2014. Salmonella typhi Samples were obtained from the Central Health Laboratory for the purpose of purification capsular polysaccharide antigen (Vi Ag). Capsular polysaccharide antigen was loaded on chitosan nanoparticle and tetanus toxoid. Recorded results showed that the antibody titers in (T1) Vi Ag + chitosan groups(620.8±364.8) were increased significantly than other groups, it included (T2) Vi Ag group (448±117.73), (T3) Vi + Tetanus toxoid group (537.6±142.53) and (T4) Vi Ag+ Ch. +TT group (121.6±38.4) at (P? 0.05).The documented results of total and differential WBCs counts were showed a significant increment in group (T1) (21020 ± 891.85 cell/1ml) compared with the control group (C) (5500 ± 230.94 cell/1ml) and other groups, it included (T3) group (10740 ± 980.61 cell/1ml), (T4) group (10650 ± 1217.78 cell/1ml) and (T2) group (9980 ± 572.18 cell/1ml) at (p? 0.05). on the other hand, the results of differential count showed elevation in percentage of neutrophils in group (T2) (40 ± 1.89%) compared with the control group (C) (33.3333 ± 0.66%) and other group, it included a (T3) group (33.6 ± 0.67%), (T4) group (34 ± 1.37%) and (T1) group (19.4 ± 0.66%) at (p? 0.05). Results also showed an increase in lymphocytes in the group (T1) (79.2 ± 2.7%) compared with the control group (58.3333 ± 1.76%) and other group, it included (T4) group (62 ± 2.06%), (T3) group (61.2 ± 2.72%) and (T2) group (55.8 ± 1.11%) at (p? 0.05).Also The study included determination the level of gene expression of two clusters of differentiation, CD25 and CD29 gene expression by RT - qPCR. The results of relative gene expression in CD25 gene showed in T4 (5.1936±2.17) were increased significantly than other groups, it included T1 (2.5604±0.64), T2 (3.7032±0.54) and T3 (4.4739±1) at (P? 0.05). The results of relative gene expression in CD29 gene which showed T3 (162.3256±89.52), increased significantly than other groups, it included T1 (33.8276±16.6), T2 (59.6817±26.65), and T4 (37.9605±3.09) at (P? 0.05).This findings were supported by the increment activity determined in lymph nodes of spleen & thymus which increased in size and numbers with active germinal centers. The overall results indicated that the use of chitosan nano particles and tetanus toxoid can enhance and improve the immune response against a given vaccines.

دراسة معالجة مياه الفضلات لمصافي بيجي وامكانية استخدامها كمياه لري بعض الخضر == Study of Wastewater Treatment For Baiji Refinery And The Possibility of Using It As Water Some Verdures

Author name: مريم عدنان ابراهيم
Supervisor name: خليل ابراهيم بندر النعيمي
General topic: Biology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت الدراسة في قسم علوم الحياة - كلية التربية - جامعة تكريت من شهر تشرين الثاني 2010 الى شهر تموز 2011 ? وكان هدف الدراسة هو معرفة بعض الصفات الفيزياوية والكيمياوية للمياه المطروحة من شركة مصافي الشمال/بيجي وامكانية استخدامها لري بعض المحاصيل الزراعية ب | This study was conducted at the Department of Biology in the College of Education - University of Tikrit. The study aims at estimating some physical and chemical characteristics of water raised from the North Refineries Company / Baiji and can be used to irrigate some crops after treatment with a number of physical methods. It also aimed at estimating some heavy elements and their impact on the growth of plants that have been studied (tomato, eggplant, sweet pepper, hot pepper). The region from which samples have been brought of the area was Albu Juwaree village that is located in the city of Baiji and adjacent to the company. Besides, it occurs near wide agricultural areas. After estimating some physical and chemical characteristics of water, soil, and the proportion of oil waste, qualities of plants were studied as well as the assessment of (carbohydrates, proteins) and some of the heavy elements and their effects on the growth of those plants.The results showed that the water of industrial waste led to a reduction in the proportion and natural growth of plants. The most affected seeds were the seeds of sweet pepper, where the total percentage of germination was(0%) when infected %)100) and the least affected seeds were the seeds of tomato, where the total percentage of germination seeds was (80%) when water is contaminated (100%). The treatment by filtering has achieved the best germination of seeds of tomato and sweet pepper, where the ratio was (100%). It turned out that contaminated water has prevented the natural growth of those plants in respect to the number and the size of leaves, the length of roots and the weight of fresh and dry leaves. The methods of physical treatment showed that lightening (25%, 50%, 75%) made to the contaminated water led to the removal of the significant effects, especially when lightening was (75%).The results indicate that there is no significant differences in the lengths of the roots of tomatoes and eggplants, except for the root of hot and sweet pepper.While the heavy elements, which was part of the study in this research, beginning with cadmium was at its highest value at the treatment by filtering and less value in (100%). Through the study it was found that the percentage of lead in all studied plants were (0.00) ppm. The Results showed that the contaminated water led to the reduction of the proportion of carbohydrates with the increase of pollution(%100), where the least proportion of carbohydrates was at the treatment by filtering. The results also showed a decrease in protein concentration with the least proportion at lightening and less value in. (25%)The results showed that the water content of contaminated industrial heavy metals studied (cadmium - lead - copper - zinc - nickel) has decreased at all treatment when treated by filtering, as well as for the remaining elements, except lead, which was (0.00) in all plants and all lightening ratios.The results of this study show that treatment of contaminated water with industrial oil waste by physical methods led to minimizing the negative impacts of such water in reducing the percentage of germination and the reduction below the natural growth compared with non - treatment water and showed that the best way of treatment for the best growth of plants were lightening with (75%) and treatment by filtering. The best response to the treatment was tomato and the most resistant to pollution were (tomato and eggplant) and the most affected by contamination and least resistant was sweet pepper.

دراسة لتشخيص بكتريا الملوية البوابية Helicobacter pylori المعزولة من الخزع النسيجية والغائط == Study For Diagnosis Helicopacter Pylori Isolated From Biopsy And Stool

Author name: ابرار علي حسين
Supervisor name: هيام عبد الرضا كريم العواد | ياسمين خضير الغانمي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Karbala
First pages:
Abstract: تناولت الدراسة الحالية عزل وتشخيص بكتريا (Helicobacter pylori) من الخزع النسيجية وعينات البرازالماخوذة من المرضى المراجعين مستشفى الحسين التعليمي حيث جمعت 122 عينة من الخزع النسيجية من وحدة الناظور من 61 مريض بواقع عينتين للمريض الواحد, كما تم جمع 228 عين | The present study addressed isolate and diagnose the bacteria (Helicobacter pylori) from biopsy and stool, samples taken from AL - Hussein Teaching Hospital where collected 122 samples of biopsy from the endoscopy. From 61 patients by two samples per patient, also were collected 228 samples of stool from the laboratory of the Department of parasites by two samples of 114 patients for the period from January 2014 to January 2015. Where shown on biopsy results which were subject to examination by the rapid urease test (RUT) the presence of 60 (98.4%) cases, while there were 23 (37.7%) case of those bacteria being positive related to biopsy was not significant differences between the two tests performed. When the distribution of test results (RUT) and culture according to age group, it appear that the highest percentage of infection was in 50 - 41 age group that which showed the highest percentage on examination (RUT) of the number of infected at 16 (26.23%) cases, either in the culture technique number of infected at 9 (14.75%) cases. But when the results of each of the examination (RUT) and culture were distribution according to the sex of the patient's infection was the higher in the females than males reaching males 28 (45.90%) cases in females of 32 (52.46%) cases for examination (RUT). No significant differences appear between the sexes in terms of infected. While the males in 8 (13.11%) and in the case of females was 15 (24.59%) case for the culture technique it did not show significant differences between the sexes in terms of the patients. While taking stool samples to check the stool antigens, it was found that there are 76 (66.7%) cases diagnosed while the technology for the polymerase chain reaction gene cagA to 49 (43%) cases have significant differences between the tests performed. When distribution the results of each of the stool antigens and polymerase chain reaction technique by age group category the highest infected was in 46 - 55 the age group with the number of 23 (20.18%) people case for screening stool antigens. The test results of the technology polymerase chain reaction showed that highest rate of infection was in the 35 - 26 age group where the number of patients was at 15 (13.16%) case.The results of the stool antigens examination also showed that the infection rate among males was higher than females, 39 males (34.21%) and in the case of females has reached the number of 37 cases (32.46%) casesThat did not show significant differences between the sexes patient. The technical results of the polymerase chain reaction by sex has been the injury was higher in females than males reaching 22 in males (19.30%) cases, while in females was 27 (23.68%) cases did not show significant differences between the sexes in terms of the infected. The results of the study conclude that (RUT) and stool antigen test is the fastest in the detection of infection from the bacteria H. pylori and then comes beyond culture technique for biopsy sample and then polymerase chain reaction for stool samples

تاثير الاجهاد المائي والسماد الورقي والمخصب العضوي في نمو نبات حلق السبع الشجيري Adhatoda vasica (L.) Nees ومحتواه من بعض المواد الفعالة وفعاليتها المضادة في بعض بكتريا تسوس الاسنان == Effect of Water Stress, Foliar And Organic Fertilizers On Growth of Adhatoda Vasica) L.(Nees. And Its Content of Some Active Ingredients And Inhibition Activity of Some Dental Caries Bacteria

Author name: ليث سريع الركابي
Supervisor name: ثامر خضير مرزة
General topic: Biology
Specific topic: Plant - Physiology
Degree: Doctorate
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: This study was conducted in Biology Department - College of Science - University of Al - Qadisiya that included pot experiment from the period of 1/4 to 1/8/2013, to assess the effect of water stress, foliar fertilizer (Foltron) and organic fertilizer (Azomin) on growth of Adhatoda vasica (L.) Nees and Its Content of Some Active Ingredients. Laboratory experiments were also done from the period of 1/8 to 1/3/2014, to determine the activity of leaves and roots extracts that resulted from plants of pots treatments to inhibit growth of some bacteria genera causing Dental Caries i.e. Staphylococcus aureus, Streptococcus mutans and Streptococcus pneumonia.A pot experiment included seventy two transplants (age of one month and 8 cm height) that were transplanted in plastic pots (one transplant per pot) in 1/4/2013. Pots were filled with sandy soil and peatmose 2 : 1 v/v. Plants Irrigated with two levels 50 and 25% of field capacity. Foliar fertilizer was used at three concentrations of 0, 3 and 6 ml/L that sprayed twice on plant shoot till complete wetness during 15/4 and 1/6/2014. Organic fertilizer was also used with irrigation water at three concentrations of 0, 4 and 8 ml/L twice on 1/5 and 15/6/2014. The design of the experiment was Randomized Complete Blocks Design (RCBD) in a factorial arrangement (2×3×3) with four replications per each treatment. Revised Least Significant Difference (RLSD) was used to compare treatment means at probability 0.05 level when treatment effect was significant.Results of pots experiment were as follows : 1 - Increasing water stress level caused a significant decrease in the most characteristics of vegetative and root growth except for stem internode and root lengths, while increasing in concentration of foliar and organic fertilizer gave the opposite effect of water stress.2 - Water stress caused a significant decrease in leaves content of total chlorophyll and nutrient elements N, P, K, Mg and protein percentage, while foliar and organic fertilizer caused a significant increase on the above characteristics.3 - Water stress caused a significant increase in proline percentage and Vit.C. and Vasicine and Vasicinone alkaloids, while foliar and organic fertilizer caused a significant decrease in proline percentage and Vit.C. and a significant increase in Vasicine and Vasicinone alkaloids.4 - The treatment of plants that were subjected to the water stress via foliar or root fertilizing or both of them caused a significant increase for most of vegetative and root growth characteristics, total chlorophyll, nutrient elements, protein percentage and Vasicine and Vasicinone alkaloids.5 - The treatment of plants that were underwent to the water stress via foliar and root fertilizing caused a significant decrease in proline percentage, Vit.C. and stem internode and root lengths.6 - The interaction of the three studied factors gave a significant positive effect to rise alkaloids of plant.Laboratory experiments included picking fresh leaves and root of plants that dried aerial in a shade, and then extracted it via methanol alcoholic of 70%, it was detected for some chemical components of extract by using reagents. Bacterial genera were isolated from the surfaces of the teeth and cultured on the growth media, and it was diagnosed by the Vitek Compact 2 System Diagnosis. The activity test involved the impact of the alcohol extract for leaves and roots to the inhibition of bacterial growth by the agar well diffusion method on Muller - Hinton.The design of the experiment was Complete Randomized Design (CRD) in a factorial arrangement (18×3) with three replications per each treatment. Revised Least Significant Difference (RLSD) was used to compare treatment means at probability 0.01 level when treatment effect was significant.Results of laboratory experiment were as follow : 1 - Increasing extracts concentration caused a significant increase in growth inhibition of the studied bacterial genera.2 - plants extraction that of the combination of A2B3C3 included (25% water stress, 6 ml/L foliar fertilizer and 8 ml/L organic fertilizer) gave the highest activated inhibition for bacterial genera compared with plant other extraction for all tested bacterial genera.3 - The plants extracted that treated by combination A2B3C3 in concentration 150 mg/ml gave higher activated inhibition for Streptococcus pneumonia reached 25.307 ml compared with Amoxicillin that reached 36.167 ml, while the inhibited extracted root reached for the same treatment 16.060 ml (Table 37 and 38).4 - Plants leaf extract was the highest effective inhibition of bacterial compared to the roots extract.

عزل وتشخيص بكتريا Streptomyces المنتجة للمضادات الميكروبية من ترب بعض اقضية محافظة صلاح الدين == Isolation And Identification of Antimicrobial Producing “Streptomyces” From Soils of Some Districts In Salahaddin Province

Author name: هيام عزيز عباس البدري
Supervisor name: عبد الكريم فتاح عمر
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تضمنت الدراسة عزل وتشخيص انواع تابعة للجنس Streptomyces، اذ جمعت 50 عينة من ترب اقضية تابعة لمحافظة صلاح الدين. شخصت?11 نوع من بكتريا Streptomyces باستخدام الاختبارات الشكلية والمزرعية والكيموحيوية حيث ظهر كل من الانواع (Streptomyces antibioticus, Strep | The Study involved ; isolation and characterization of species related to Streptomyces. Fifty samples of soil has been taken from different districts of Salah aldden province. The isolates were identified by the morphological, biochemical tests and classified to nine species, The following species were found : (Streptomyces antibioticus, Streptomyces coelicolor , Streptomyces distalicus and Streptomyces fluvissimus ) in Tikrit district, ( Streptomyces cyaneus ) in Ballad and beiji districs, ( Streptomyces exofolicus) in Ballad and Aldor districts (Streptomyces lavendulea ) in Samarra and Aldor districts , (Streptomyces purpureus ) in Samarra and Beiji districts and (Streptomyces violaceus ) in Samarra and Tikrit districts. The sensitivity of these bacterial isolates were tested toward the following : (Azithromycin, Cefotaxime, Cephatexin, Doxycycline, Erythromycin , Gentamicin, Neomycin, Penicillin, Tobramycin and Vancomycin).All species showed high resistance to cefotaxime. But all species were sensitive to neomycin and doxycycline. The isolated species of Streptomyces were tested for their ability of antibiotic production. It was found that (Klebsiella) was sensitive to all the species except species Streptomyces coelicolor. Streptomyces coelicolor showed an effect against Proteus. While Staphylococcus aureus was sensitive to the following species (Streptomyces antibioticus, Streptomyces cyaneus and Streptomyces purpureus). The Scanning Electron Microscopy was used to study the surface of spores. The species of (Streptomyces antibioticus) was found to has warty surface while spores of ( Streptomyces coelicolor) has smooth surface.

دراسة بعض المتغيرات الفسلجية وتركيز البروستاكلاندينات عند مرضى الربو

Author name: سيماء عبد الرحمن شعبان
Supervisor name: زيد محمد مبارك المهداوي | صالح محمد رحيم العبيدي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت هذه الدراسة لمعرفة بعض المتغيرات الدموية والفسلجية عند مرضى الربو، وقد شملت 46 مريضا بالربو من كلا الجنسين (31 مريضا من الاناث و15 مريضا من الذكور) تراوحت اعمارهم بين 21 - 75 سنة من ضمن الحالات الواردة على مستشفى تكريت التعليمي والمركز الاستشاري لام

معالجة مطروحات مياه فضلات الصرف الصحي لمدينة تكريت باستخدام تقنية المجففات الحرارية الدواره واعادة استخدامها كاسمدة عضوية == Treatment of Domestic Wastewater Effluent of Tikrit City Using Thermal Rotating Dryer Technique And Reuse As Organic Fertilizers

Author name: هالة ارشد علي حسين
Supervisor name: جهاد ذياب محل الجنابي
General topic: Biology
Degree: Doctorate
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت الدراسة في مختبرات كليتي التربية والعلوم - جامعة تكريت للفترة من نيسان 2010 لغاية شباط 2011 تتضمن الدراسة معالجة الحماة الناتجة عن حوض التجميع الابتدائي باستخدام تقنية المجففات الحرارية الدوارة للعمل على ازاحة ماء الحماة والتي تمثل اصعب العمليات الت | This study was conducted at a period from April 2010 to February 2011, to treat the sludge produced from collection tank at tikrit domestic wastewater plant, using thermal rotating dryer technique(T.R.D) or dewatering the sludge.This process represents one of the difficult applied process for the sludge treatment. This technique considered a modern technique in this field.Physical, chemical and biological characteristics of the manufactured sludge were determined and compared with organic fertilizers (I.O.F) imported from republic of Estonia (Available in local markets).T.R.D technique induced dewatering of the treated sludge at ratio48.5 - 61.5% and producing highly sterilized organic fertilizers which was free of pathogenic organisms(bacteria, worms and eggs) with high chemical characteristics comparing with those imported from Estonia. Manufactured organic fertilizers M.O.Fs have allow acidity pH ranged between 7.5 - 7.7 ,. electrical conductivity at range 1630 - 1873, organic matter at ratio 4.56 - 5.35%, with a moderate concentrations of Pb, Cd, Zn.The concentration of N and K was 1.8, 5.96% respectively while the concentration of phosphorus are 350 ppm. Three levels of M.O.Fs were used for corn plants(5, 10 and 20ton/acres). Results showed that all these levels significantly induced increasing for some physiological and morphological characteristics of corn plants compared with those treated by I.O.Fs or control plants. The vegetative dry weight of corn plants fertilized by M.O.Fs were 23.95gm/plant compared with 16.3 and 5.3 gm/plant for plants fertilized by I.O.Fs and control respectively. (M.O.Fs) induced significant increase in nitrogen concentration compared with plants fertilized by I.O.F and control while the concentrations of Pb, Cd, Zn were not affected by using of M.O.Fs on the other hand Pseudomonas aeruginosa and Escherichia coli were contributed in reducing and removing some of pollutants from the sludge at three incubation periods (30, 60 and 90 day). These two species caused significant decrease in pH and EC of the sludge, P. aeruginosas showed the highest removing ratio of nitrogen (93.1%). The cooperation of the two species caused high removing ratio for the Total organic carbon(87.72%) at 60 days and organic materials (90.68%) at 90 days of incubation also showed high removing ratio for phosphors 100%) at 90 days of in cubation.) These two species also caused high removing ratio for pb, Cd and Zn at30 days and P. aeruginosa was more effective in removing Pb, Cd and Zn from the sludge then E. coli.

انتاج انزيم الفوسفاتيز القاعدي من العزلة المحلية لبكتريا Bacillus sp. وتنقيته جزئيا

Author name: جعفر انور قدوري الخاجي
Supervisor name: رباب عمران راضي الجيلاوي
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: Fifteen local isolates of Bacillus bacteria and previously isolated were screening a from laboratory of advanced biotechnology and Genetic Engineering in Babylon University, College of Science to know their ability on production of extracellular alkaline phosphatase after its growth in liquid production in PH8 medium for 4 days and temperature 37°C. and destinguished 11 isolates by their different abilities on produc of enzyme and Bacillus sp.I bacteria was their best and perfected it selection to complete the study and described by depending on microscopic and cultured and biochemical characteristics studying of effect some of environmental condition for production of alkaline phosphatase enzyme by submerged cultural method were appeared that the optimum PH for production was 8, and optimum temperature 40°C, after incubation time 4days and explain that the production by static incubator was better from shaker incubator.Alkaline phosphatase of the isolate Bacillus sp.I was purified partial by fractionating with ammonium sulphate and the best ratio for saturation 95%. And on purification of enzyme by ion exchange chromatography with batch wise by using DEAE - cellulose and enzymatic recovery was 37.1%, and fold 3.64 once, and enzyme passed after concentrated on gel filtration column (Sepharcyl S - 200) one peak was appeared for enzyme and enzymatic recovery was 23 % and fold 36.80 once.Results of electrophoresis by presense of denaturation condition appeared that the molecular weight of alkaline phosphatase produced from gel filteration by using Sepharcyl S - 200 gain approximately 29.5 KDa and 28.8 KDa and this enzyme composed of homodimer.The characterization results for the enzyme indicated that the optimal pH for activity of enzyme was (9), while the optimal pH for the enzyme stability was (8 - 9), and found that the optimal temperature for activity of anzyme was 30 ? C, and on studying optimal temperature for stability of enzyme indicated that better stability for this enzyme was on temperature 0 ?C while the activity lost completely on temperature 60°C. and on studying effect some chemical compounds in activity of enzyme found that the enzyme needs to cofactor and it is zinc andmagnessium ion to increase catalytic activity and needs to calessium ion to increase stability of enzyme. At that time potassium and sodium ions appeared a negatively effect on activity, and heavy metals ions Fe and Hg appeared an inhibited effect to activity and so that the enzyme was inhibited by presence EDTA and and potassium ferro cyanide indicating that the enzyme from metal enzymes.Determination of constant kinitics of the enzyme was completed of Michaelis - Menten constant (Km) of para - nitrophenyl phosphate and this gained 500 micromolar and maximum velocity of this enzyme (Vmax), and this gained 2500 unit/ml.

دراسة تشخيصية وتصنيفية لطفيليات الديك الرومي Meleagris gallopavo في محافظة القادسية == Diagnostic And Taxoaomical Study of Turkey (Meleagris Gallopavo) Parasites In Al - Qadisiya Province

Author name: هند عبد الزهرة عبد الكاظم الشباني
Supervisor name: هادي مدلول حمزة الميالي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: خلال المدة ما بين بداية شهر ايلول 2014 ونهاية ايار 2015 جمع وفحص 50 طيرا من الديك الرومي Meleagris gallopavo من مناطق مختلفة من محافظة القادسية في مختبرات الكلية بهدف عزل الطفيليات الخارجية والداخلية المتطفلة على هذه الطيور وتشخيصها فضلا عن دراسة نسبة الا | The current study carried out during the period between the first of the September 2014 end of May 2015.fifty birds of domestic turkey Meleagris gallopavo was collected from different areas of Al - Diwaniya city markets, to isolating and identification the external and internal parasites parasitized on these birds as well as study the incidence and intensity and the most important pathological and histological changes resulting from external and intestinal infections, Six species of external parasites was isolated included four species of biting lice Gonoiocotes gallina, Goniodes gigas, Menacanthus straminus and Oxylipeurus sp. The infection rate and severity of 10%, 6.81.4%, 4.72.2, %, 54.2%, 0.09, respectively, and Two species of Ticks are Argus persicus and Haemophysalis sp. Nymph. and the rate of infection was 2%, 0.36; 2%, 0.24 : respectively. The results showed that the triple infection of lice are the most common, (63.63%.) While internal parasites that 9 species of intestinal parasitic worms in the digestive system included two species of Protozoa Eimeria sp. and Histomonas meleagridis The infection rate and intensity was14%, 4.20 0.2%, 0.14, respectively, Four species of tape worms Railletina cesticillus, Railletina tetragona. Choanoyania infundibulum and Hymenolepis sp.The intensity of infaction was 2%, 100.2% 53.284, 4, 00, 2% 2%, 0.42; respectively, Three species of nematodes Ascardia gallinarum, Capillaria sp. and Heterakis gallinarum and severity of infection was 21.73%, 1.2; 2% 0.100; 17.79%, 1.25; respectively. The study showed the presence of internal parasitic worms sites in the gastrointestinal tract it was observed that most of tapeworms parasitize in the small intestine and rates higher than what is found in other parts of the gastrointestinal tract, and the protozoa are found in the liver and small intestine. The nematodes are also found in various parts of the gastrointestinal tract, including both of the small intestine and the large intestine and esophagus. The species Haemophysalis sp. and Oxylipeurus sp. and helminths Raillietina cesticillus, Raillietina tetragona, Choanotania infundibulum, Hymenolepis sp. Ascardia gallinarum, Capillaria sp.Heterakis gallinarum. and Eimeria sp. were the first recording in turkeys in Iraq. Also the current study showed a many of gross and microspcopic pathological changes caused by lice in infected birds, such as lethargy, laziness and sagging wings and nervous tension and break the feathers and hair loss, as well as change the color of the skin also appeared many of the macroscopic and microscopic changes in the gastrointestinal tract as a result of, intestinal worm included changes macroscopic obstruction and hemorrhagic bruises and blood infiltration The microscopic changes have included the infiltration of inflammatory cells Nicrosis and abstraction of cells lining the of these channels and the proliferation of Kubffer cells and expansion in sinosoids and atrophy of the villi tissue.

التاثيرات السمية لجسيمات الفضة النانوية على بعض الاعضاء لاناث طائر السمان Coturnix coturnix : دراسة نسجية وكيموحيوية == Toxic Effects of Nanosilver Particles On Some Organs of Female Quail (Coturnix Coturnix); Histological And Biochemical Study

Author name: عبير ساجد عبد علي
Supervisor name: غزوة درويش النقيب
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: تضمنت هذه الدراسة التعرف على تاثير جسيمات الفضة النانوية في نسجية كل من الكبد والطحال والكلى فضلا عن تاثيرها في وظائف الكبد والكلى من خلال قياس بعض انزيمات الكبد المتضمنة (AST & ALT) وقياس مستوى اليوريا والكرياتنين (Urea & (Creatinine, فضلا عن تاثيرها في | This study was included to identify the effect of silver nanoparticles on histology of the liver, spleen and kidney, as well as it's effect on functions of the liver and kidneys through the measurementof some liver enzymes included (AST and ALT) and measurement of urea and creatinine levels, as well as the effect of silver nanoparticles in the weight of studied samples, numbers and sizes of the eggs were produced through the exposure periods.(45) quail (females) were collected from agricultured research center in Abu - Ghraib, divided into (4) groups including : T1 (12 quails were exposed to 4ppm), T2 (12 quails were exposed to 8ppm) and T3 (12 quails were exposed to 12 ppm) of nanosilver particles solution for (60) days.As well as control group T4(9 birds were exposed to distilled water).After the end of the exposure period samples were sacrificed by discapitation of the neck, and blood was collected for the purpose of serum separation which required for biochemical study, samples were dissected and fixated with Bouin's fluid, histological slides were prepared and stained with Eosin - Hematoxylin.Biochemical study were showed significant decrease in the level of liver's enzymes (AST & ALT), urea and creatinine. This decrease was elevated when the concentration was increased, as well as significant decrease in each of sizes and numbers of the eggs were produced through exposure periods and non - significant decrease in the body weight.Exposed groups with silver nanoparticles showed histopathological alterations for each of liver, spleen and kidney, in liver included congestion of blood vessels, infiltration of inflammatory cells, exudate, appearing of concilman bodies, fatty degeneration in most of hepatocytes which progressed to fatty changes in most of samples were exposed with 12ppm, Hyperplasia for each of blood vessel walls and capsule, atrophy of hepatocytes, and appearing of giant cells, while kidney sufferd from hydropic degeneration in most of renal tubules hemorrhage for each of cortex, medulla and glomerulus, hyaline degeneration of tubules, amyloid precipitation for each of glomerulus and blood vessels, sloughing of epithelial cells of renal tubules from basement membrane, hypertrophy of renal tubules with hyperplasia for cells of tubules and atrophy of renal tubules.Spleen tissue showed decreased in sheets of white pulp and lymphocyte numbers red pulp cells. Severity of these effects varied depending on individual differences between samples and concentration of silver nanoparticles, which increase in samples were exposed with highest concentration.

استعمال التقانة الجزيئية في تشخيص التباين الوراثي في تراكيب وراثية من الحنطة المتحملة للملوحة == Using Molecular Biology In Identification of Genetic Variation In Wheat/Genotypes For Salt Tolerance

Author name: غفران علي حسين العبيدي
Supervisor name: وسام مالك داود | ابراهيم اسماعيل المشهداني
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: This study is made in bio - technology Research Center/Al - Nahrain University 2012 - 2013 for heredity genetic variation by using RAPD and ISSR method between tow genotypes elected to status salinity tolerance 2H and N5 and local varieties Iraq and Latifia sensitive to salinity.Tow experiments are made, first for measure the percentage of germination under conditions of salinity, being of planted the seeds of genotypes and local varieties studied in three replicates and three salt levels 0, 16, 12 ds.m - 1 by 10 seeds in each experimental unit, and after 10 - 15 days of agriculture was to estimate the percentage of germination and the second experiment to study the genetic variation between varieties tolerant and sensitive to salinity, where its seeds are planted soil saline by tow salt concentrations 0, 20 ds/m and after 20 - 25 days of germination, took samples of the leaves of plants to extract the DNA for study genetic variation using RAPD - PCR technique between varieties tolerant and sensitive to salinity.The results of the percentage of germination of the existence of significant differences between genotypes salt - tolerant 2H and N5 and cultivars comparing Iraq and Latifia sensitive to salinity, gave genotype N5 highest percentage of germination was 71%, and gave the genotype 2H germination percentage of 62% in the salt level of salt 16 ds/m, While giving classes comparison Iraq and Latifia less germination percentage reached 16.5% and 25% at the same level of salt, also gave genotypes 2H and N5 germination percentage of 66% and 75%, respectively, while giving classes comparison Iraq and Latifia germination percentage of 50% for both cultivars in the Second level of salt, It is clear from these results that the genotypes N5 and 2H elected from breeding programs and improvement are the most salt - tolerant varieties of local Iraq and Latifia sensitive to salinity at the stage of germination and which is the stage mostsensitive to salinity of the stages of growth of other, particularly in the third level of salt 16 ds/m.The results show that the interaction of RAPD - PCR using 7 primers and there are differences between genotypes N5 and 2H and local varieties Iraq and Latifia and varied this primers in terms of the number of bands and its location was the initiator of OPC - 12 is the best among the primers being of manages to show the discriminative power of through its production bands with a molecular weight 100bp in genotypes N5 and 2H under conditions of salinity just did not show this bands in the local varieties Iraq and Latifia and under the same conditions of salinity, This indicates that this band represents a source of difference between genotypes and local varieties in the degree of salinity tolerance because this band does not appear in all genotypes and cultivars studied under non - saline conditions, it may return this deals band in genotypes and N5 2H under conditions of salinity to show Jenny expression by some of the salt - tolerant gene, which leads to the appearance of this genetic variation in salinity tolerance status.Has also been used 15 primers to determine the genetic variation technique ISSR - PCR between genotypes N5 and 2H and local varieties Iraq and Latifia sensitive to salinity, appeared a number of bands general, however, these primers cannot show any special bands, and thus these primers failed to show this genetic variation between species studied.We conclude by the results that the genotypes 2H and N5 were the most tolerant to salinity at the stage of germination and who have shown a special bands under conditions of high salinity, while the local varieties are sensitive to salinity not appeared in this band under the same conditions and by this difference in the appearance of this package can We concludethat there is a genetic variation between these genotypes and local varieties this may be due to their differences in the degree of salinity disclaim.

تاثير بعض عوامل المكافحة الحيوية في بعض الجوانب الحياتية للذبابة المنزلية Musca domestica L. (Diptera : Muscidae) == Effect of Some Biological Control Agents On Some The Biological Aspects of Musca Domestica (Diptera : Muscidae)

Author name: علي مرتضى كاظم الياسري
Supervisor name: محمد رضا عنون الحسناوي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: تضمنت الدراسة الحالية تقويم كفاءة بعض طرائق السيطرة الحيوية باستعمال الفطر Sorokin Metschnikoff)) Metarhizium anisopliae والبكترياBacillus thuringiensis var.israelensis ومنظم النمو Neporex Cyromazine) ) في المكافحة الحيوية للذبابة المنزلية Musca domestica | The present study aimed to evaluation of efficacy of some methods Metarhizium anisopliae Sorokin) Metschnikoff) Bacillus thuringiensis var.israelensis and Neporex) Cyromazine) from be used as biocontrol agents against (Diptera : Muscidae)Musca domestica L. The results showed the following : 1. Different concentration of Fungal suspension have been affected the life stages of Musca domestica. The mortality rate of eggs were (18.66%) at the concentration of 2 × 103 spore /ml, and increased to (36.66%) at the concentration of 2 × 106 spore /ml. The first larvae instars have showed highest mortality rate reached (83.33%) when treated with 2 × 106 spore/ml, while (56.66%) of them were dead at concentration of 2×103 spore/ml. (36.66%) of pupae of Musca domestica were dead when exposed to 2 × 106 spore /ml. The treatment with highst concentration caused high adult mortality, i.e. (96.66%) and (93.33%) for adult males and females of the Musca domestica respectively after 168 hours, while (70%, 73.33%) when treated with 2×103 spore /ml. 2. The secondary metabolities of fungi when applied, all first larvae instars of Musca domestica were dead and (96.66%) of the when tretead with concentration 100%, while (66.66%) and (63.33%) for the same larval instars at concentration 25%. 3. The result showed the different concentration of bacterial suspension affected the mortality rate of larvae of Musca domestica.The first larva instars have showed highst mortality rate which reached (76.66%) when treated with 2 × 105 spore /ml while (50%) of them where dead at concentration 2 × 102 spore /ml after 72 hours from treatment. 4. The effect of secondary metabolites of bacteria when applied, All first larva instar of Musca domestica were dead and (90.00%) when treated with concentration 100% and decreased to (73.33%) after 72 hours from treatment , the mortality rate of adult was (100%) at concentration 100% and decreased to (63.33%) after 72 hours from treatment. 5. The concentration of insect growth regulator neporex have effected , The highst mortality rate eggs was (37.00%), at the concentration 100 part per million, while (19.33%) at the concentration 25 part per million, , the first larval instars mortality at the concentration 100 part per million, was (96.66%) at the concentration 100 part per million while (66.66%) at the concentration 25 part per million.the mortality rate of pupa was (73.33%) while (30%) at the concentration 25 part per million after 72 hours from treatment

اثر التغذية الورقية بالبوتاسيوم والحديد المخلبي في نمو وحاصل الذرة الصفراء (mays L. Zea) تحت نظام الري بالتنقيط == Effect of Foliar Nutrition of Potassium And Chelated Iron In Growth And Yield of Corn (Zea Mays L.) Under Drip Irrigation System

Author name: ايمن احمد عبد الكريم العباسي
Supervisor name: نجم عبد الله جمعة الزبيدي
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: نفذت تجربة حقلية في محطة ابحاث محاصيل الغالبية /محافظة ديالى في الموسم الخريفي 2013في تربة ذات نسجة طينية غرينية بهدف معرفة تاثير التغذية الورقية بالبوتاسيوم والحديد المخلبي في نمو وحاصل الذرة الصفراءL.) Zea mays (صنف بحوث 106 تحت نظام الري بالتنقيط بتصمي | A field experiment was conducted during autumn season 2013 in silty clay soil at Field Crops Researchs Station in AL - Ghalbiea/ Diyala Governorate.The aim of this experiment to study effect of foliar nutrition of potassium and chelated iron in growth and yield of maize(Zea mays L.) Buhooth 106 under drip irrigation system.Random Complete Block Design (RCBD) was used in this study with three replications. Spraying four concentrations of potassium(0, 1000, 2000, 3000) mgK. L - ¹ as potassium sulfate K%41.5 and four concentrations of chelated Iron EDTA(Fe%13)(0, 50, 100, 200)mg Fe.L - ¹. The results showed that the suitable) K (foliar nutrition treatment was 3000mgK.L - ¹ which caused significant differences in plant height, number of leaves, stem diameter, leaf area, dry matter of green plant, ear length, number of rows per ear, number of grain per ear, weight of 500grains, grain yield, chlorophyll, concentration of K and concentration of Fe as compared with control, it in order to195.87cm, 15.51 leaf, 27.32mm, 59.11dm2, 6017g.m2, 20.158cm, 17.025row, 516.75grain, 143.75g, 9.507t.ha - 1, 51.34 SPAD units, %3.003, 145.36mg.Kg - 1.Using Fe - foliar nutrition treatment was 50mgFe.L - ¹ caused significant differences in plant height and number of leaves it 193.76cm and 15.54 leaf، and 100mgFe.L - ¹ caused significant differences in stem diameter, leaf area, dry matter of green plant, ear length, number of rows per ear, number of grains per ear, weight of 500grains, grain yield, chlorophyll and concentration of K as compared with control it in order of 27.76mm, 60.41dm2 , 6004g.m2, 20.95cm, 17.15 row, 505.33 grain, 143.00g , 9.646 t.ha - 1, 50.08 SPAD unit, 2.9284%.While 200mgFe.l - ¹ caused low of all traits parameters except concentration of Fe in leaves was 188.18 mg.Kg - 1.The interaction between potassium and iron K3000 * Fe100 of significant effect in more traits parameters compared with control treatment and concentration of potassium decreased high level of iron(200mgFe.L - ¹) of monitor form

تعيين بعض الظروف الزرعية لانتاج انزيم السليليز والكحول الاثيلي من بعض انواع البكتريا المعزولة من التربة == Determination of Some Cultural Conditions For Cellulase And Ethanol Production By Bacteria Isolated From Soil

Author name: عبد الستار عبد الجبار ابراهيم
Supervisor name: عدنان نعمة عبد الرضا العزاوي | انيس عبد الله كاظم
General topic: Biology
Specific topic: Microbiology - Enzymes
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: The study included isolate and diagnose of some types of anaerobic and aerobic bacteria capable of decomposing and fermentation of cellulose. Isolation and diagnose the bacteria Clostridium phytofermentous from 10 agricultural soil samples, was 50 % of the total 50 colony, while isolation of Escherichia coli from 15 waste water samples which represented 40% of the total 75 colony and isolated Pseudomonas aeruginosa from 15 normal soil samples, and was 53% of the total 75 colony. production of cellulase enzymes was evaluated by using absorbance method. Higher activity obtained via Clostridium phytofermentous with maximum value of 42.8 IU / mL, while Pseudomonas aeruginosa gives 12.5 IU / mL and Escherichia coli gives 29.1 IU / mL.. Optimum temperature was 35 C and pH 7, for production cellulose enzyme The concentration of ethanol Alcohol was measured by using Titration method. Maximum concentration of ethanol alcohol produced by Escherichia coli, which reached 8.28 g/liter, while Pseudomonas aeruginosa was 7.86 g/liter, and Clostridium phytofermentous reached 6.62 g/L.

دراسة بيئية للطحالب الملتصقة على بعض النباتات المائية في مياه مبزل الفرات الشرقي (الحفار) / الديوانية - العراق == An Ecological Study of Epiphytic Algae On Some Aquatic Plants In East Euphrates Drainage Water /(Al - Haffar) Al - Diwaniya/Iraq

Author name: زهرة كليب مهدي الخزعلي
Supervisor name: فؤاد منحر علكم
General topic: Biology
Specific topic: plant - aquatic life
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: تناولت الدراسة الحالية الخصائص البيئية المؤثرة على الطحالب الملتصقة على النباتات المائية في مبزل الفرات الشرقي (الحفار) في الديوانية - العراق, اذ شملت الدراسة قياس بعض الخصائص الفيزيائية والكيميائية لمياه المبزل والخصائص الاحيائية التي تضمنت دراسة كمية ون | The present study tackles ecological characteristics which is effected of Epiphytic algae in east Euphrates drainage (AL - Haffar) in AL - Diwaniya, This study included the measurement some of physical and chemical characteristics of drainage water, and biological characteristics which includes studying quality and quantity of Epiphytic, Three aquatic plants Ceratophyllum demersum, Phragmits australis, and Imperata cylindrical had been selected, Samples collected monthly during The period from November 2012 to October 2013.Three location have been chosen, the first is near AL - Abtan village, Second is after the first location five kilometers, while the third location is after the Second about seven kilometers. The study shows that the temperature of the air is about (7 - 39c) and water temperature is approximately (10 - 33c), while light penetration is about (10.5 - 63cm) the flow speed is (0.25 - 0.68 M/Sec), Water turbidity is (40.02 - 63.03 NTU), while Electrical conductivity is approximately about (3014 - 3964 Sc/cm). The results of the study shows that the water drainage Oligohaline, the salinity values ranged from (1.92 - 2.53‰), the total (T.D.S) is about (1741 - 2226 mg/l), while the T.S.S. is about (41 - 67 mg/l). The result of this study shows that the pH is limited Through all the period of the study (6.98 - 8.18), The drainage water get good airing dissolved oxygen values are(6.71 - 8.14 mg/l), biological oxygen demand are (2.05 - 5.23mg/l) and carbon dioxide values are about (81.50 - 119.57mg/l).The results of this study also show about that drain water is alkaline and belonged to bicarbonate ions, total alkaline is (140.28 - (189.27 mg/l) It is discovered that the drain water is too hard, hardness values are (512.15 - 612.45mg/l), while Calcium concentrations are (128.36 - 173.40mg/l) and Magnesium concentrations are (30.31 - 50.09 mg/l). Plant nutrients, N concentrations are about (0. 98 - 3.99 µg/l) of Nitrit, Nitrate concentrations are (216 - 415 µg/l) and Phosphate concentrations are about (0.9 - 2.92 µg/l), and The percentage of total organic carbon in the residues is(0.59 - 0. 17) %. The total number of epiphytic algae types which are diagnosed during study period reaches to 320 species belongs to 86 genera within 7 classes They are Bacillariophyceae, Chlorophyceae, Cyanophyceae, Euglenophyceae Xanthophyceae, Dinophycea eand Rhodophyceae, Bacillariophyceae obtaints the dominion which reach to (183) species of Bacillariophyceae. It makes rate (57.19%).Than Chlorophyceae came concerning types number 68 species in rate (21.25%) , Following by Cyanophyceae 53 species in rate 16.56%. Euglenophyceae are 10 species in rate (3.13%).Xanthophyceae is represented in two species belong to2 genera.It makes proportion (0.63%) , Dinophyceae which represented in 3 species belonged to 3 genera in rate (0.94 %) and Rhodophyceae represented in one species and one genera in rate (0. 3%) of total number of algae.Class of the Bacillariophyceae, during study period were Navicula وNitzschia وCymbella وSynedra وAchnanthes, genera of Oscillatoria and genera of, Scenedesmus manifest that highest number of available types during the period of study.It has note that there are numbers of the monthly and located changes in total number of epiphytic algae on host plants.was varied in months and locations due to the host plants. Ceratophyllum demersum marks the Highest total number of epiphytic algae 172.89× 104 individual/ gm of wet weight in third location during April. Imperata cylindrica marks the lowest total number of epiphytic algae 3.51×104 individual/ gm of wet weight in scecond location during January, The total number of epiphytic on host plants ranges between (175.39 - 668.6) ×104 individual/ gm of wet weigh.The total number of main epiphytic algae classes is varied in total number of epiphytic algae.Bacillariophyceae diatoms composed 81.02%, in all locations during the study period, followed by Cyanophyceae composed 9.7%, while Chlorophyceae composed 8.6%.Euglenophyceae, Chrysophyceae, Dinophyceae and Rhodophyceae, were not important concerning their number. Statistical analysis results show there are significant differences between locations and months of study in relation to physical, chemical and biological features. Also there are connected correlations positive and negative between epiphytic algae and physical, chemical and biological features that measured during the study period of probability level of P<0.05.
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