First pages:

دراسة بعض الجوانب المناعية لاصابات اللشمانيا في عينة المرضى العراقيين == Study of Some Immunological Aspects of Leishmaniasis In Sample of Iraqi Patient

Author name: مرتضى قيس علي العامري

Supervisor name: انوار ادريس سليمان العساف

General topic: Biology

Specific topic: Microbiology - Immunology

Degree: Master

University: University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology

Language: Arabic

University location: Baghdad

First pages:

Abstract: This study was carried on 145 patients infected with leishmaniasis including; 75 with CL, 70 with VL, attending Al - Karama Teaching Hospital, Central Public Health Laboratory and Central Pediatric Teaching Hospital, during the period October 2013 to February 2014 were enrolled in this study, in addition to 30 healthy control collected from the National Center for blood donation for the same age groups for a group of patients. No clinical autoimmune manifestations such as arthralgia or cutaneous vasculitis were reported in any patients. Based on direct skin smear examination and serum samples analysis, the patients were distributed into two clinical groups : 30 patients suffering from cutaneous leishmaniasis and 30 patients suffering from visceral leishmaniasis patients.The presented study aimed to confirmation of diagnosis cutaneous leishmaniasis depending on direct skin smear and visceral leishmaniasis based on indirect immunofluorescent assay test IFAT, then investigate the association of IgG autoantibodies against some antigens, especially : nuclear antigens, like : ds - DNA, nucleosome, histones, SmD1, PCNA, ribosomal P0, SS - A/Ro60kD, SS - A/Ro52kD, SS - B/La, CENP - B, Scl70, U1 - snRNP, AMA M2, Jo1, PM - Scl, Mi - 2, Ku and against cardiolipin, in order to correlate the types of diseases presentation (cutaneous or visceral) with the presence of these autoantibodies in a sample of Iraqi patients.The study was reached to the following results : 1. The direct microscopically examination of skin smear in CL patients, showed positive results in 30 cases (40.0%) compared to 45 cases (60.0%) negative results, and was a significant difference at the level of (P?0.05).2. The anti - leishmanial antibodies by Indirect immunofluorescent assay test in VL patients, showed positive results in 30 cases (42.9%) compared to 45 cases (57.1%) negative results, and was a highly significant difference at the level of (P? 0.01).3. The age groups most affected in CL were 16 to 40 years, with high significant differences at the level of (P? 0.01) were observed when compared with the other age groups. As for patients with VL, who aged between 3 months to 9 years, they showed variable significant differences between age groups in female while there were no significant differences in male at the level of (P?0.01). The majority of patients were aged 3 to 4 years and they represent 9 (30.0%). The study result showed 16 CL patients (53.3%) have only one lesion, while 11 patients (36.7%) have 2 to 9 lesions and only 3 cases (10%) have ? 10 lesions in different sites of their bodies. There were elevated high significant differences at the level of (P?0.01) when compared between the groups. And 21 cases (70%) have infected for ? two months, and the remaining 9 cases get their infection for > 2 months duration. There were increased high significant differences observed at the level of (P?0.01) when compared between the groups. Dry lesions recorded superiority in 20 (66.7%) of the CL cases, and other 10 (33.3%) have been diagnosed as a wet lesions. There were elevated high significant differences observed at the level of (P?0.01) when compared between the two types of the lesion. And The upper limbs were the dominant site of lesions in the CL cases, 15 (39.4%) of the cases had their lesions in one or both hands, followed by the lower limbs in 11 (29.0%) cases and the less frequent site of infection was the face in only 4 (10.5%) cases and 8 (21.0%) cases common lesions. The results showed obvious high significant differences at (P? 0.01) between the groups.4. For the tests of the autoantibodies, the anti - ds - DNA antibody was positive only in one patients (3.3%) with CL of the total (30) patients, while tests of the rest 29 (96.7%) were negative, while in VL of the total (30) patients, 9 patients (30.3%) were positive and 21 (70.0%) were negative. The anti - ds - DNA in leishmaniasis patients were highly significant at the level of (P?0.01) when compared between the study groups. As for the anti - nucleosomes antibodies, it was positive in 5 patients (16.7%) and negative in 25 (83.3%) among patients in CL, while in VL patients, 16 patients (% 53.3) registered positive and 14 (46.7%) their result werenegative, the anti - nucleosomes antibodies in leishmaniasis patients were highly significant at the level of (P?0.01) when compared between the groups study. No score was recorded for anti - SmD1antibodies in CL patients, no one was positive 0 (0.0%) and all the 30 patients (100.0%) gave negative result for this antibody, while in patients with VL, the result showed 4 patients (13.3%) gave positive and 26 patients (86.7%) were negative, the anti - SmD1antibodies in leishmaniasis patients was significantly different at the level of (P?0.05) when compared between the groups. While the anti - SSA/Ro60kD and anti - SSA/Ro52kD, in VL patients, 9 patients (30.0%) scored positive results and 21 of the patients (70.0%) were tested negative for anti - SS - A/Ro60kD, but 7 patients (23.3%) were positive and 23 of the patients (76.7%) were tested negative for anti - SS - A/Ro52kD. While in patients with CL, no one scored positive (0.00%) and 30 of the patients (100.0%) were negative for anti - SS - A/Ro60kD, whereas one patient (3.3%) gave a positive result and 29 of the patients (96.7%) were negative for anti - SS - A/Ro52kD. About anti - SS - B/La we found that 19 of the patients (63.3%) were scored positive result for this antibody in addition to 11 patients (36.7%) who were negative in VL patients and 4 of the patients (13.3%) have those antibodies and 26 of the patients (86.7%) haven’t these antibodies in CL patients respectively. The anti - SS - A/Ro60kD, anti - SS - A/Ro52kD and anti - SS - B/La in leishmaniasis patients were highly significantly different at the level of (P?0.01), when compared between studied groups.5. VL cases showed a significantly different distribution of anti - nuclear antibodies (ANA) in compare with CL cases, especially; in Anti - Ku antibodies 24 (80%), anti - Mi - 2 antibodies 21 (70%) and anti - SS - B/La antibodies 19 (63.3%) respectively.6. The results of anti - nuclear antibodies level in patients with leishmaniasis like; anti - ds DNA, anti - nucleosome, anti - SmD1, anti - PCNA, anti - SS - A/RO 60, anti - SS - A/RO 52, anti - CNP - B were in high significantly different at the level of (P?0.01) and level (P?0.05) when compared between the groups, while the other autoantibodies like; anti - histone, anti - ribosomal P0, anti - Sc170, anti - U1 - snRNP, anti - AMA M2, anti - J0 - 1 and anti - PM - Sc1 showed no significant differences (P?0.01) between the investigated groups.7. The mean value of anti - cardiolipin IgG antibodies was increased substantially with highly significantly differences (P?0.01) in VL patients as compared with control group (8.123 vs. 1.959), also as compared with CL patients (8.123 vs. 2.402).

دراسة تاثير جنس الجنين وبعض المتغيرات الفسلجية في تراكيز بعض الهرمونات والمتغيرات الاخرى في دم الحوامل لمدينة تكريت وضواحيها == Study The Effect of Fetus Gender And Some Physiological Variables On The Concentrations of Certain Hormones And Other Variables On Pregnant Blood In Tikrit City And Its Suburbs

Author name: مروه عصام سليمان

Supervisor name: وهبي عبد القادر سلمان

General topic: Biology

Specific topic: Zoology - Physiology

Degree: Master

University: Tikrit University - Faculty Of Education - Department Of Biology

Language: Arabic

University location: Salahaddin

First pages:

Abstract: جرت هذه الدراسة في مختبرات مستشفى تكريت التعليمي ومراكز الرعاية الصحية ومختبر علوم الحياة في كلية التربية للبنات جامعة تكريت وضواحيها للفترة من تشرين الاول2011 ولغاية اذار 2012 لمعرفة تاثير مراحل الحمل وعوامل جنس الجنين وكتلة الجسم الحي وعمر الام والسكن ل | This study was conducted in the laboratories of Tikrit Teaching Hospital, health - care centers, and the laboratory of Biology in college girls education in Tikrit city and its suburbs from the period of October 2011 until March 2012. The study was performed to see the impact of the pregnancy stages; sex factors of the fetus; body mass index; the mother's age; and residence for pregnant women, in the concentration of : certain blood components (Hb, PCV, WBC); blood sugar; cholesterol; estrogen, progesterone and testosterone hormones; and (TSH, T3, and T4 hormones. The study included (90) women at different stages of pregnancy, and the number of pregnant women at every stage was (30), ranged their ages from (15 - 40) years. A sample of (10) ml blood was taken from the surveyed women. The serum was separated by the common methods of separation, and conducted thereon desired examinations, The outcomes were as follows : - 1. Superiority of the first and second stages of pregnancy significantly (P<0.05) in the concentration of (Hb, PCV), compared with the third stage. 2. Increasing the total count of white blood cells and the concentration of blood sugar and cholesterol significantly with the progress of pregnancy stages. 3. Increasing progesterone hormone concentration significantly with the progress of pregnancy stages, while the concentration of estrogen hormone decreased in the middle stage of pregnancy, and increased significantly in the first and third stages, whereas there were no significant differences in the concentrations of testosterone hormone during the different stages of pregnancy. 4. The concentration of TSH and T3 hormones were decreased significantly with the progress of pregnancy stages, while the concentration of T4 hormone was increased in the first and last stages of pregnancy.5. Superiority of male pregnancy significantly compared with the female pregnancy in the concentrations of Hb and PCV ratios during the first and second stages of pregnancy, while the opposite happened in the third stage.6. Superiority of male pregnancy significantly compared with the female pregnancy in the total count of white blood cells during the first and third stages of pregnancy.7. Superiority of male pregnancy significantly compared with the female pregnancy in the concentrations of blood sugar, while the female pregnancy surpassed significantly in the concentrations of cholesterol compared with the male pregnancy during all the pregnancy stages. 8. Superiority of female pregnancy significantly compared with the male pregnancy in the concentration of estrogen hormone in first stage, while the opposite happened in the third stage, while the male pregnancy surpassed significantly compared with the female pregnancy in the concentrations of testosterone hormone during second and third stages, as well as superiority of female pregnancy significantly compared with the male pregnancy in the concentration of progesterone hormone during the first and third stages of pregnancy, while the opposite happened in the second stage.9. Superiority of female pregnancy significantly compared with the male pregnancy in the concentrations of TSH hormone during the first stage of pregnancy, while the opposite happened in the third stage. while Superiority of male pregnancy significantly compared with the female pregnancy in the concentrations of T4 hormones during the first and second stage of pregnancy but the concentration of T3 superiority all pregnancy stages.10. A significant decrease in the values of Hb and PCV with increasing the body mass index during the second stage of pregnancy while the opposite happened in third stage of pregnancy.11. Increasing the total count of white blood cells significantly with increasing the body mass index in the first stage of pregnancy, while the opposite happened in the last stage of pregnancy. Increasing the concentration of blood sugar with increasing body mass index in first and second stages of pregnancy but concentration cholesterol decreasing significantly with increasing the body mass index.12. Decrease the concentrations of estrogen hormone significantly during first and second stages of pregnancy with increasing the body mass index for pregnant women, while a significant increase appeared in the concentration of progesterone hormone with increasing the body mass index during the second stage of pregnancy, and the opposite happened during the first and third stages. Testosterone hormone increased significantly with increasing the body mass index for pregnant women during first and second stages of pregnancy 13. A significant increase appeared in the concentrations of TSH hormone at pregnant women with decreasing the body mass index. while a significant increase for the concentration of T3 in first stage and T4 hormone increase at pregnant women during all stages of pregnancy with increasing the values of body mass index.14. No significant differences in values of Hb and PCV during the first and second stages of pregnancy for all ages, but the small ages surpassed significantly compared with the old ages during the third stage of pregnancy. while The total count of white blood cells decreased significantly with the progress of pregnant women's age during all pregnancy stages.15. The small age groups for pregnant women increased significantly in the concentration of blood sugar during the first stage of pregnancy, while they decreased significantly during the second stage of pregnancy.while Age did not affect significantly in the concentrations of cholesterol during the first stage of pregnancy, while the old age groups decreased significantly compared with the first and second age groups) 32 - 24), (23 - 15) during the second and third stages of pregnancy.16. The concentration of Estrogen hormone increased significantly with the age progress during the first and third stages of pregnancy compared with the second stage, while the concentrations of progesterone hormone decreased significantly at old ages during the first and third stages of pregnancy.The concentration of testosterone hormone was not affected significantly during the last stage of pregnancy for all ages, but it decreased significantly during the second stage of pregnancy compared with the first stage of pregnancy for all ages.17. A significant increase showed in for the concentration of TSH in first and the last stages while decreased significantly the concentration T3 and T4 hormones with the age - progress at all stages of age.18. No noteworthy impact for residence of pregnant women in all stages of age, cause its impact is very little on all the qualities covered in the present study.

تاثير بعضز الظروف البيئية على النظام الدفاعي المضاد للتاكسد خارج الخلوي لدى المدخنين والعاملين في مجال اللحام والاشعاع == Effect of Some Environmental Conditions On The Extra - Cellular Defense System Among Smokers And Workers In The Field of Radiation And Welding

Author name: معد رشيد مطلك الزبيدي

Supervisor name: مازن رزوقي محمد

General topic: Biology

Specific topic: Zoology - Physiology

Degree: Master

University: University of Diyala - College Of Education For Pure Sciences - Department Of Biology

Language: Arabic

University location: Diyala

First pages:

Abstract: تعد ظاهرة الاجهاد التاكسدي من اهم الميكانيكيات المحتملة لاحداث الضرر في النظام الحيوي عند التعرض لانواع مختلفة من المؤثرات والعوامل البيئية، ولذا تكون الانظمة المضادة للتاكسد هي اول من يتاثر بالزيادة الحاصلة في تكوين الجذور الفعالة المؤكسدة. لذلك تهدف هذ | The phenomenon of excessive oxidation considered the most important mechanism that cause potential damage to vital system when exposed to different forms of environmental factors, so anti - oxidants systems are the first to be affected by the increase formation of oxidizing radicals. For this reason, This study aims to determine the extent of the effect of some environmental conditions on the Extra - cellular antioxidant system, determine the most affected part in this system, study the correlation of this damage with other variable and to identify the natural values of different antioxidant system components. The study was carried out in the district of Baquba city, capital of Diyala province during the period from 10 October 2013 to 1 May 2014, the study group included (160) individual divided into four group (40) persons working in the field of welding, (40) persons workers in the radiation and (40) persons smokers and compared with (40) healthy people (the control group), all individual included in this study were male with age rang (25 - 45 years).Requested blood samples were taken for laboratory testes including complete blood count, and measurement of the following biochemical parameters, total serum protein, serum albumin, serum zinc, serum copper, serum iron, serum glutathione and malondialdehyde. The results of the laboratory test elicited that, the most common abnormalities in this study were the presence of low level of glutathione zinc, and copper in individual in all three study group in compare with controlled group and the differences were statistically significant(p value<0.05, <0.001, <0.01 respectively), also low level of iron in radiation group in compare with controlled groups and the differences were statistically significant(p value<0.01), and low level of proteins and albumin in smoking group in compare with controlled group and the differences were statistically significant(p value<0.01).also results of the current study revealed increased in the level malondialdehyde (MDA) in all three study groups in compare with controlled group and the differences were statically significant (p value<0.01). and increased levels of protein and albumin in the radiation and welder group in compare with controlled groups and the differences were statistically significant(p value<0.05) and increased in the level of iron in welder and smokers compare with control group and the differences were statistically significant (p value<0.001). The results of other laboratory test which include the complete blood count show High levels of white blood cells in all study groups compare with control group and the differences were statically significant (p value<0.001), and High levels of neutrophil and lymphocyte in welder and radiation group compare with control group and the differences were statically significant (p value<0.001). Results of the current study also showed increased levels of hematocrit, and red blood cells count and platelets count in a smokers group compare with control group and the differences were statically significant (p value<0.01).also there is increased in red blood cells count in radiation group in compare with controlled group (p value<0.05) , and decreased in platelets count in radiation group in compared with controlled group (p value<0.05).

تاثير المعاملة ببعض مبيدات الافات والمواد الكيمياوية في نمو الفطرين Alternaria tenuissima وFusarium oxysporum المرافقة لبذور وجذور الحنطة Triticum aestiumn == Effect of Some Pesticides And Chemicals In The Growth of Fungi Alternaria Tenuissima And Fusarium Oxysporum Associated With The Seeds And Roots of Wheat (Triticum Aestiumn)

Author name: مهند جاسم حمود

Supervisor name: عبد الامير سمير سعدون

General topic: Biology

Specific topic: Microbiology - Fungi

Degree: Master

University: University of Al-Qadisiyah - Faculty Of Education - Department Of Biology

Language: Arabic

University location: Qadisiyah

First pages:

Abstract: اذ تم في هذه الدراسة عزل عدة انواع من الفطريات المرافقة لجذور وبذور الحنطة تموز 2 مصدق 1وتم تشخيص تسعة انواع من الفطريات المرافقة للبذور وبنسب تردد مختلفة وهذه الانواع هي Aspergillus niger, Penicillium notatum , Alternaria tenuissima, Rhizopus stotanifer | As it was in this study to isolate several types of fungi associated with the roots and seeds of wheat July 2 certified 1 was diagnosed nine types of fungi associated with seeds and different frequency rates and these types areAspergillus niger, Penicillium notatum, Alternaria tenuissima, Rhizopus stotanifer P. digitatum, Fusarium oxysporum, A.flavus, Fusarium solani, A. alternata. Was diagnosed nine types of fungi isolated from the roots of wheat and the surrounding soil and different frequency rates and these types. A. alternata, A. niger, R. stolanifer, Mucor sp., Trichoderma hazianum, P. notatum, F. oxysporum, F. solani, Alternaria tenuissima. Testing was diagnosed two fungal species tested using a technique monomer chain reaction PCR. These species are fungal Fusarium oxysporum, Alternaria tenuissima. This study tested the effect of pesticides is a fungal pesticide jungles Granstar and insecticide Wide. The transactions chemical which urea and NaCl in the growth of some fungi isolated from the seeds and roots of wheat as it has been test the effect of different treatments and their interaction in the germination of seeds of wheat on the filter paper and compare nets comparison innate Elsa (Carbendazim) and underwent tests the effects of different treatments and their interaction in radial growth and dry weight of the two types of fungi isolated in the germination of their spores and bacterial length of the tube, as well as test the effect of transactions and their interaction in the germination of seeds in the soil sterile and non - sterile. The results showed that the use of urea and sodium chloride concentration of 15 mg / mL in the treatment of seeds of wheat led to increased germination of these seeds and the impact of a negative in some types of pathogenic fungi associated with them as well as the use of pesticides other than fungal pesticide jungles Granstar and insecticide Wide negative effect also in the vitality Activity and fungi.germination The results showed the existence of significant effects on rates of seeds treated with pesticides and salts on the filter paper analogy transactions comparison since given concentration 15 mg / 10 mL of pesticides and 15 mg / ml of salt are high in the germination of the seed treatment, which exceeded ratios germination of seeds of wheat treatment urea fertilizer treatment fungicide Elsa. As rate ratios germination of wheat seed treatment with urea 96.66%, while the rate ratios germination of seeds of wheat pesticide Elsa 93.33%, followed by treatment of pesticide Wide as rate ratios germination 83.33% and 80.00% for wheat seed when the focus 15 mg / ml of sodium chloride salt treatment, and after treatment Karan Star as the rate of germination rates of seeds of wheat 73.33%. The results showed the existence of significant effects in increasing the proportion of seed germination treatment with pesticides and salts in the soil sterile and non - sterile as it gave the Focus 15 mg / 10 mL of pesticides and 15 mg / ml of salts highest rates of germination of these seeds came these results are compatible with the results of seed germination in the filter paper. The results show that as well as the individual transactions were more influential than the transactions overlap in radial growth and dry weight of fungi testers and their spores germination rates and lengths of germ tubes, as growth rates of radiographic Fungi F. oxysporum and A. tenuissima in the treatment of a mixture Wide and Granstar 11.44, 8.55 mm, respectively, while the rates of diameters of the colonies for the treatment of urea pesticide Wide 33.66 and 26.66 mm, while the pesticide mixture Wide treatment with sodium chloride amounted to 37.00 and 15.00 mm, in while rates were diameters of colonies for the treatment of a mixture Granstar with sodium chloride, 52.66 and 25.00 mm, and the growth rates were radiography for the treatment of pesticide Granstar with urea 25.00 and 11.66 mm, and the growth rates for the treatment of sodium chloride with urea 31.24 and 37.33 mm, compared with the fungicide Elsa, which amounted to the radial growth rates of 8.55 - 11.44 mm Fungi testers respectively.

تقويم صفة تحمل بعض اصناف البطاطا المكثرة نسيجيا للجفاف على المستوى الجزيئي == Evaluation of Some Potato Varieties Grown In Vitro To Drought Tolerance At The Molecular Level

Author name: هوازن حليم صالح

Supervisor name: علي عبد الامير مهدي الصالحي

General topic: Biology

Specific topic: Biotechnologies

Degree: Master

University: University of Baghdad

Language: Arabic

University location: Baghdad

First pages:

Abstract: درست استجابة اربعة اصناف من البطاطا Solanum tuberosum L. وهي (Lusa, Ambo, Arizona, Riviera)، استجابتها للزراعة النسيجية، وتقييم صفة تحمل الجفاف، كما اجريت التحاليل الجزيئية لتحديد البصمة الوراثية لها.اوضحت نتائج تجربة اختبار استجابة الزراعة النسيجية، ان ا | Four varieties of Potato (Solanum tuberosum L. Lusa, Ambo, Arizona and Riviera) were studied for their response to tissue culture and evaluation of the treatment of drought tolerance and molecular analysis. Results showed that the varieties differ in their response to growth, where as Arizona was the best variety in shoot tip culture and the survival percent (100%) and gave the highest average of branch length and number of leaves which were 9.810 cm, 8.100 leaf/ plant, while Riviera showed lower rate of survival and growth (40%). Riviera was excluded from the experiments. Cytokines and auxins were used in the following concentrations Kinetin (kin) with 2, 4 mg/L and Benzel adenine (BA) with interacted with Indoleacetic acid (IAA) at 1 or 2 mg/l or Napthalene acetic acid (NAA) at 0.5 or 1.0 mg/L in multiplication experiment. Arizona variety exhibited the highest length shoot, number of nods, number of leaves, root length, dry weight of roots, fresh and dry weights of shoots when using kinetin 2mg/L; interacted with NAA at 0.5 mg/L. while it showed a significant increase in the average of branches number, stem height, fresh and dry weights of shoot as well as the number of roots after treatment with using BA at 1 mg/L in combination with 0.5 mg/L (IAA). Drought tolerance of the studied varieties was studied by using Poly Ethlene Glycol (PEG) at the concentrations 20, 40, 60, 70, or 80 g/l. It showed that proline content was at its highest in Ambo variety reached 22.811 ? /g at the concentration 80 gm/L PEG. Arizona gave the highest mean in number of branches, number of nods, number of leaves, number of roots, under water stress conditions when the medium was supplemental with 20 mg/L PEG. Molecular analysis the studied varieties was conducted by using 6 primers. Results showed that the inheritance approximation between them, 0.314, 0.297, 0.273 for Lusa, Ambo and Arizona respectively. These results indicate that Rivera variety is genetically different from the others.

دراسة جزيئية للـ Bacteriocine المنتج من قبل بكتريا lactobacillus salivarius == Molecular Study of Bacteriocine Produce By Lactobacillus Salivarius

Author name: ايمان حمزة محمد

Supervisor name: احلام نعيم كاظم الياسين

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Kufa - College Of Education For Girls - Department Of Biology

Language: Arabic

University location: Najaf

First pages:

Abstract: هدفت الدراسة الحالية التحري عن قابلية عزلات بكتريا Lactobacillus salivarius على انتاج البكتريوسين ودوره في تثبيط فعالية بعض العزلات البكتيرية السالبة لصبغة غرام. جمعت 155 عينة من مصادر سريرية مختلفة شملت 20عينة مسحات مهبلية، 8 عينة مسحات اللعاب، 6عينة حل | The aime of study detecting the a bility of Lactobacillus salivarius to produce bacteriocin and determine it’s role in inhibition the activity of some Gram negative bacteria. Atotal of 155 sample from different clinical source has been collected during the period from October - 2012 to March - 2013, these include 20 samples from vaginal smear, 8 samples from saliva and 6 samples from human milk.The results of primary isolation, Laboratory diagnosis and biochemical test showed that 34 isolates has bee identified as L.salivarius.Ahigh precent of isolation has been carried out from human milk which was 40%. The ability of all isolates to produce gelatinase and biofilm has been detected and the results revealed the ability of some isolates to produce gelatinase and biofilm. Ahigh percent of gelatinase production has been detected in L.salivarius isolated from milk sample (50%), while alow percent of gelatinase production was 0% in L.salivarius isolated from saliva. The results of optical density of biofilm produced by L.salivarius isolates from milk which was 100% higher than it in isolates from vaginal and saliva which was 85% and 75% respectively. All isolates shows antibacterial effect against some Gram negative bacteria, the diameter of inhibition as between (2 - 8), the results showed the ability of 4 selective isolates of L.salivarius to produce bacteriocin in which the Rf valve of precipitate bacteriocin was (0.42 - 0.6) compard with leaky bacteriocin hich was (0.57 - 0.8), also leaky bacteriocin as more activity against G - ve bacteria than precipitate bacteriocin. The result of plasmid profile in L.salivarius revealed the presence of megaplasmid in all isolate except one which had one megaplasmid and one small plasmid. Bacterial conjugation between L.salivarius that produce bacteriocin and had megaplasmid which used as adonor cell with E. coli Jm 109 as a recipient cell showed transferring of megaplasmid from donor to recipient cell and the transconjugant cells showed high anti - bacterial effect due to bacteriocin production against G - ve bacteria. The result of amplification of Sal P gene encoding salivracin P by PCR confirmed that all isolate had no Sal P gene in there genetic materials which revealed that these isolates had another type of bacteriocin rather than Salivracin P.