University location: Qadisiyah

First pages:

Abstract: The aim of This study is to determine the prevalence of OXA ? - lactamase in pseudomonas aeruginosa isolates from clinical and other environmental cases in Al - Diwaniya Teaching Hospital using phenotypic and molecular methods.The Samples of the study were collected from various sources and they were 390 samples for the period from November 2011 to March 2012, and they includ (292 clinical specimens and 98 environmental sample), the results of cultural and biochemical tests showed that 50 isolates (39 of them were from clinical cases and 11 were from environmental samples), belonged to ps. aeruginosa, and their diagnosis were confirmed by 16s - ribosomal RNA, and the study showed that all the isolates containing the 16s - ribosomal RNA gene, which represents the designed diagnostic gene in this study.The results of this study showed that the higher rate of P. aeruginosa isolates was due to burns infections (23.68 %) , followed by respiratory tract infections (sputum) that were (15.38 %) and then the case to otitis media infections (12.5 %), and lastly that due to urinary tract infections (10.49 %), while the rate isolation from the environmental samples was (5.11 %) from the floor of burns department, followed by (10.8 %) from the medical instruments of department workers.It was noted from this study that the age and gender of the patients have some effect on the infection by P. aeruginosa it was recorded that the higher rate of infection was in patients older then 61 year old, and regarding gender, it was found that females are more infected than male, especially in urinary tract infections. The study showed that there is high rate of infection in the admitted patients, in older people and in those who need urinary catheterization, in addition to those with severe burns and those in the intensive care units. Drug sensitivity test had been carried out for all the bacterial isolates to 22 types of antibiotics by disk diffusion method of Kirby - Bauer, and this study had indicated that there was relatively high resistance from P. aeruginosa to ? - lactam antibiotics, aminoglycoseides, and fluoroquinolone. The results also showed that the studied isolates has possibility of producing broad - spectrum ? - lactames enzymes, and this is supported by the resistance of this bacteria to the third generation cephalosporins and to Alaztronam, it was also shown that there was difference in the resistance to carbapenems antibiotics, the resistance rate to imipenem and meropenem were 16% and 64 %, respectively. Polymyxin B and Polymyxin E also called (Colistin) antibiotics were also used, and the resistance rate %0 for Colistin and %4 for Polymyxin B, that means there was two isolates which are resistant to Polymyxin B and this resistance was a challenge to the success of therapeutic efforts. this study noted that there was 22 (44%) isolates which were resistant to at least three types of antibiotics, and regarded as multi - drug resistant (MDR) and Extensive - drug resistance (XDR) isolates were26 (52%) which was the higher rate among the three types of resistance, The third type of resistance was 2 (4%) which represents the resistance to all types of studied antibiotics (PDR). The ability of these isolates to produce broad - spectrum OXA ? - lactames enzymes groups was investigated thrrough detection of presence of genes blaOXA - 10, blaLCR - 1, blaOXA - 18, blaOXA - 1, blaOXA - 2 in these isolates by using the polymerization chain reaction enzyme technology] (PCR) it showed 50/50 (100%) isolates contain blaOXA - 10 gene, which belongs to the OXA group I, and the results of this study showed no amplification results for blaOXA - 18, blaLCR - 1, blaOXA - 1, blaOXA - 2 genes, which belong to the main groups of broadspectrum OXA ? - lactames enzymes which are OXAgroup II, OXAgroup III and OXA groupV respectively except OXA18 enzyme

دراسـة مظهرية وتشريحيـة مقارنة للاجناس Sophora L. وTaverniera DC. وSecurigera DC. (Leguminosae) في العراق == Morphological And Anatomical Study of Genera Sophora L., Taverniera Dc. And Securigera Dc.(Leguminosae) In Iraq

Author name: زهراء قاسم عبد محسن الكلابي

Supervisor name: ازهار عبد الامير سوسة

General topic: Biology

Specific topic: Plant

Degree: Master

University: University of Al-Qadisiyah - Faculty Of Education - Department Of Biology

Language: Arabic

University location: Qadisiyah

First pages:

Abstract: تضمن البحث الحالي دراسة مظهرية وتشريحية لثلاث اجناس للعائلة البقولية Leguminosae في العراق وهي Securigera DC. وSophora L. وTaverniera DC.، ودرس نوع واحد من الجنسين الاول.securidaca S والثالث T.nummularia ونوعين من الجنس الثاني So.japonica وSo.gibbosa.تم | The present research included Morphological and Anatomical study for three genera Securigera DC. , Sophora L. and Taverniera DC. belong to the family Leguminosae in Iraq. Four species it was taking one species of the first genus S.securidaca and third genus T.nummularia , two species from the second genera So.japonica, So.gibbosa. The study of Qualitative and quantitative characters of vegetative and reproductive parts were also study and discuss The variation in These qualities. Morphological traits were compared in terms qualities of characters of Habit and Duration , stems, leaves, stipules, inflorescences , bracts and parts floral , fruit and indumentum. Species were separated and placed in groups depending on the obvious differences and similarities these qualities as some of morphological characteristics such as the shape of Leaves, if possible to isolate species T.nummularia it have Unifoliolate Leaves and the outer species have Imparipinnate and the rest of stipules shape in isolate species S.securidaca if was a shape Foliar and other species was Spiny and other qualities. other variations have been discussed with in the qualities. And it became clear of Habit and Duration , stems, leaves, stipules, bracts and parts floral, fruit were of considerable taxonomic value. The study also involved anatomical characters of different organs particularly the leaf epidermis, indumentum, cross section of the leaves, petioles and stems also venation.as some Anatomical characters like species S.securidaca for the rest of the species it Undulate wall clearly been under review also benefit from cross section of the stems as possible to isolate species S.securidaca it was Hollow and empty Pith from the cinter either species was Solid if occupies the Pith status. show that many of anatomical characteristics specially the stem, leaf and also the shape of the walls of epidermal cells and some qualities indumentum taxonomic importance were assessed. Taxonomic treatment has been carried out for all genera and their species and a key was designed for them. with description for each genus and species in this study.

التاثير القاتل لمستخلصات نبات اليوكالبتوس Eucalyptus camaldulensis في يرقات بعض انواع البعوض (Diptera : Culicidae) في التجمعات المائية لمزارع القمح والشعير في ضواحي مدينة الكوت - العراق == Extracts Killer Effect of Eucalyptus Camaldulensis Plant On Larvae of Some Mosquito Species (Diptera : Culicidae) In Aquatic Pools of Wheat And Barley Farms In Suburbs of Kut / Iraq

Author name: زينب محمد عباس

Supervisor name: جميل سعد السراي

General topic: Biology

Specific topic: Zoology

Degree: Master

University: Wasit University - College Of Science - Department Of Biology

Language: Arabic

University location: Wasit

First pages:

Abstract: شملت الدراسة جمع عينات من يرقات الطور الثالث والرابع لبعض انواع البعوض من الجداول المائية لمزارع القمح والشعيرفي مدينة الكوت - واسط / العراق خلال الموسم الزراعي الممتد من تشرين الثاني 2013 ولغاية نيسان 2014 ومن ثم تقييم فاعلية المستخلص المائي والكحولي لاو | The present study was established to evaluate the effectiveness of the water and alcohol extracts of Eucalyptus camaldulensis Dehnhardt Leaves on the killing of the 3rd - 4th instar larvae of some mosquito species collected from Wheat and Barley fields in AL - Kut city, Wasit province / Iraq, during the period of six months (November 2013 - May 2014). This study has been demonstrated the following results : The study showed the presence of three types in the study aquatic environment distributed on gender, type I return to the genus Aedes, a type Aedes caspius pallas and appeared during months November 2013 to February 2014. The types other two Faaudan to genus Culex two type Culex tritaeniorhynchus Giles and Culex pipines L has appeared during March and April 2014, respectively, of a monthly period extending from from November 2013 to the end of the study in April 2014. The results of the study showed the water and alcohol extract leaves the plant E. camaldulensis kill larvae in the third and fourth instar of the three types of mosquitoes effect. The following : - The alcohol extract of Eucalyptus camaldulensis leaves was more effective on the mortality of mosquito larvae than water extract after 24h following treatment. In which the (10000 ppm) at the highest concentration of both extracts (alcohol and water) showed the highest response. The mortality rate of larvae Ae.caspius of alcohol and water extracts were (100, 90) % respectively. As well as the alcoholic LC50 and LC90 that were given to same species, were calculated and the results were (8, 2700 ppm) respectively. Whilst the water extract at the same lethal concentrations findings were (2500, 9900 ppm) respectively. - The mortality rate of larvae Cx. tritaeniorhynchus of alcohol and water extracts were (73, 66) % respectively. As well as the alcoholic LC50 and LC90 that were given to same species, were calculated and the results were (6400, 12500 ppm) respectively. Whilst the water extract at the same lethal concentrations findings were (5600, 11400 ppm) respectively. - The mortality rate of larvae Cx. pipines of alcohol and water extracts were (43, 3.3) % respectively. As well as the alcoholic LC50 and LC90 that were given to same species, were calculated and the results were (16100, 22300ppm) respectively. Whilst the water extract at the same lethal concentrations findings were (zero, zero) respectively.

دور الفئران المنزلية Mus musculus والجرذان النرويجية Rattus norvegicus في وبائية بعض الامراض الطفيلية من اوالي الدم والانسجة في مدينة تكريت وضواحيها == The Role of Mus Musculus And Rattus Norvegicus In The Epidemiology of Some Blood And Tissues Parasite Diseases of Protozoa In Tikrit City And It'S Districts

Author name: شيلان قادر صادق الصالحي

Supervisor name: توفيق ابراهيم محمد الالوسي

General topic: Biology

Specific topic: Life Science

Degree: Master

University: Tikrit University - Faculty Of Education - Department Of Biology

Language: Arabic

University location: Salahaddin

First pages:

Abstract: اجريت الدراسة في مناطق وبعض اقضية محافظة صلاح الدين للفترة من كانون الاول 2006 الى الفترة تشرين الثاني 2007، وتم فحص 200 نموذجا من الفئران المنزلية Mus musculus و50 نموذجا من الجرذان النرويجية Rattus norvegicus جمعت من ثماني مناطق وهي حي شيشين وحي الجمعية | The study was carried out in various regions of Selah El - Deen from the period 12 - 2006 to 11 - 2007. In this research a total of two hundred Mus musculs and fifty Rattus norvegicus from eight regions of Selah El - Deen area, namely, Sheshen area, Al - Jamiea area, Al - Sikak area, Al - Kadisea, Al - Alban Factory, Al - Dor, Samara and Al - Alam were investigated. The examination results of the brain, liver, spleen and blood smears of each rodent were showed 4 species of parasitic protozoa : 1. Toxoplasma gondii : This species found in Mus musculs only with the percentage of infection (2.0%).2. Leishmania spp. : This species found in Rattus norvegicus only with the percentage of infection (2.0%).3. Eperythrozoon coccoides : This species found in the blood of Mus musculs only with the percentage of infection (1.5%).4. Encephalitozoon cuniculi : This species found in Mus musculs with the percentage of infection (3.0%), and in Rattus norvegicus with the percentage of infection (4.0%).The percentage of infection in Mus musculs was (6.5%) but the percentage of infection in Rattus norvegicus was (6.0%).Encephalitozoon cuniculi appeared in a high percentage of infection in both Mus musculus and Rattus norvegicus.The experimental results of heart and lungs tissues for all rodents studied was negative. The statistic results showed no significant difference in percentage of infection among species of protozoa in Rattus norvegicus, but there were significant difference in percentage of infection among species of protozoa in Mus musculus.There were no significant difference in percentage of infection between collection area of Rattus norvegicus, but there were significant difference in percentage between collection regions of Mus musculus.The seasons of year had no significant difference on the percentage of infection with Rattus norvegicus, but there were significant difference in percentage between the seasons of year in Mus musculus.Rodents sex showed no significant difference in percentage of infection in Mus musculus and Rattus norvegicus

دراسة تاثير مستخلصات القرفة (Cinnamomum zeylanicum) والسماق (Rhus coriaria) في التثبيط المايكروبي وبعض المعايير الحيوية في ذكور الجرذان == Study The Effects of Cinnamon (Cinnamomum Zeylanicum) And Sumac (Rhus Coriaria) Extracts On Microbial Inhibition And Some Biological Parameters In Rats Male

Author name: فراس عدنان حسين الزيدي

Supervisor name: كركز محمد ثلج الجبوري

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: Tikrit University - Faculty Of Education - Department Of Biology

Language: Arabic

University location: Salahaddin

First pages:

Abstract: صممت الدراسة في مختبرات قسم علوم الحياة / كلية التربية وقسم علوم الاغذية / كلية الزراعة في جامعة تكريت, اذ تضمنت الدراسة التعرف على المواد الفعالة في المستخلصات المائية والكحولية في كل من قلف القرفة وبذور السماق واختبار فعاليتها التثبيطية عند تركيز 10, 25 | This study was conducted in Biology department laboratory / Education college and Food science department in Agriculture college in Tikrit University. the study was conducted to investigated to the aquaies and alcoholic activities extracts from each Cinnamon bark and sumac seeds and assay of inhibition activity concentration at 10, 25, 50 and 100 mg/wells against gram positive and negative bacteria and phagocytosis Immunity level after injection by E.coli type, further more to study the effect it in some physiological parameters in male rats after oral administration for 8 days by 50 and 100 mg/kg from rats body weight from each aquais and alcoholic extracts. The results was illustrated the aquaies and alcoholic extracts from each Cinnamon bark and sumac seeds were contains the active groups from compound such as Tannins, Resins, Phenols, Flavonoids, Alkaloids, Glycosides, Coumarins. while the effects was found the aquaies extracts a high significantly effects (p < 0.05) from alcoholic by inhibition ability when assay. The Minimum inhibition concentration (MIC) from each ones which mass 50 and 125 mg/ml from culture media to inhibition concentration by E.coli and S.pyogen cinnamon bark aquaies extract. it was 75 and 100 mg/ml from culture media to inhibition concentration by E.coli and S.pyogen from alcohol extracting its inhibiting efficiency throw the measure of the diameter of the inhibiting area that has shown that the gratest effect was E.coli bacteria which its diameter of inhibiting area 17 mm and when the concentration is 100 mg/wells from the alcoholic and aquaies extracting and the less effect is S.pyogen and S.aeruginosa in case of the extraction from cinnamon bark it was 17 mm the diameter of inhibiting area of E.coli bacteria. when the concentration 100 mg/wells from aquaies extracting from sumac. whether with alcohol extraction, the bacterial type were similar in their sensitivity in diameter of inhibiting from 18 to 20 mm but the oral administration of the extract on the level of phagocytosis process through the injection of rats in affixed number from E.coli bacteria. The result was illustrated the type of concentration from each cinnamon bark and sumac seeds such as aquaies and alcoholic was caused an increase in the phagocytosis process through the significantly decrease in the number of bacteria with increase of the concentration from the groups that oral administration from extraction that there number wear (zero) on the concentration 100 mg/kg from rats body weight when the time is 120 minute which was equal with effincy of the ciprofloxacin antibiotic on the concentration 5 mg/kg from rats body weight. The result also showed the effect of oral administration from the extraction of cinnamon bark and sumac seeds such as aquaies and alcoholic, that significantly caused the decreased in the concentration of the hemoglobin and Total count red blood cells and the packed cell volume on the concentration 100 mg/kg from the weight but the measures have not significantly changed the parameters MCV, MCH, MCHC with increase of concentration from extraction compared with the group of controlling. The white blood cells also decreased in the significantly aquaeis and alcoholic extracting from the cinnamon bark it was 6.8 × 103/mm3 on the oral administration of the rats from the concentration 100 mg/kg from the body weight of the aquaeis extracting, and 6.8 × 103/mm3 on the oral administration of the rats from the concentration 100 mg/kg from the body weight of the alcoholic extracting, That numbers have not affected during the oral administration from the extractions of sumac only on the concentration 100 mg/kg from the body weight of the alcohol extracting which was 5.6 × 103/mm3 the increase was in the ratio of the number of the neutrophils in the whole extraction alcoholic and aquaies for the both of plants. While the effect of oral administration from the extraction in the lipids profiles was clear in the decrease significantly in the concentration of the glucose and albumin and Urea and uric acid and creatinine and triglyceride and, cholesterol, and the low density lipoproteins (LDL) and very low density lipoproteins (VLDL), significant increasing in globulin and high density lipoproteins (HDL) in aquaies and alcoholic extraction of sumac seeds while the value of Total protein and HDL is not significantly differtited in aquaies and alcoholic extraction moreover oral administration of the both of plants. The enzyme activity value was included significantly decreased of the Alkaline phosphtase activity value with the increased orally administration from aquaies and alcoholic extracts from both of plants, while increased significantly Aspartames amenotransferase (AST) activity value in all cinnamon extracts while was significantly decreased when administrate from sumac seeds extract, But don’t significantly differ Alanine amenotransferase (ALT) activity value in all concentration from aquaies and alcoholic extracts from both of plants.

Author name: نورس مجيد حميد الخفاجي

Supervisor name: علي عبد الكاظم الغانمي

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

Language: Arabic

Full text from the source: https://uokerbala.edu.iq/wp-content/uploads/2020/07/Rp-Preparation-of-Two-Nanohybrid-Compounds-from-Chlorhexidine-and-Tannic-Acid-and-Determination-Their-Antimicrobial-Activities-Against-Microorganisms.pdf

University location: Karbala

First pages:

Abstract: تضمنت هذه الدراسة عزل وتشخيص اهم انواع البكتريا المسببة لاخماج الحروق كما تضمنت ايضا تحضير وتشخيص مركبين نانويين هجينين احدهما من المطهر كلورهكسدين والاخر من حامض التانيك. تم تقييم الفعالية التثبيطية للمطهر النانوي المحضر اعلاه ضد البكتريا المعزولة في هذه | This study included the isolation and diagnosis of the most important species of bacteria responsible for the infection in burns. It also included the preparation and detection of two hybrid nano compounds, one of them from the disinfectant chlorhexidine and the other from the tannic acid. The inhibitory activity of the nano disinfectant was assessed against the isolated bacteria in this study also, the antioxidant activity of the hybrid nano tannic acid was identified and the results showed the following : 1 - As a total 32 samples were taken from burned patients hospitalized in the burning ward Imam Al - Hussien medical city in Sacred Karbalaa governate. It had been obtained 8 isolates (34.78) % from Pseudomonas spp., 7 of them were Pseudomonas aeruginosa and one was P. oryzihabitans , 4 isolates (17.39) % were Acinetobacter baumannii, 4 isolates (17.39) % were Klebsiella pneumoniae, and one isolate (4.34) % of Proteus mirabilis, Escherichia coli, Serratia liquefaciens and Chryzeobacterium meningosepticum and two isolates (8.69) % of Staphylococcus aureus in addition to one isolate (4.43) % from Candida guilliermondii yeast.2 - Chlorhexidine disinfectant and tannic acid were used in preparation of the two hybrid nano compounds after intercalating each of them on layers of double hydroxide (Mg/Al - LDH). FT - IR spectrum studies has shown the appearance of specific chemical groups and the disappearance of others while the spectrum of x - ray(XRD) has shown the appearance of new levels of diffraction in the spectrum of the hybrid nano disinfectant Mg/Al - CHX - LDH and the spectrum of the hybrid nano tannic acid Mg/Al - TA - LDH as compared to the spectrum of the Mg/Al - LDH, which indicate that the two prepared compounds under study is a nano hybrid compounds. Results of the scanning electronic microscope has shown, the change in the shape of the surface of the nanohybrid compounds that prepared above as compared to the layer of double hydroxide. It turns out from the results of the examination with the use of Atomic Force Microscope (AFM), that the diameter of the particles of hybrid nano disinfectant and hybrid nano tannic acid were (121.66 and 77.70) nm, respectively. 3 - The inhibitory activity of the hybrid nano disinfectant Mg/Al - CHX - LDH and the free disinfectant (Free CHX) has been studied and the results revealed followings : a. The hybrid nano disinfectant has the highest inhibitory activity against the two bacterial isolates P. aeruginosa (11 and 23 - A) with an inhibition diameter of 24 mm for each, while the inhibition diameter for the free disinfectant was 23.5 mm against the isolate P. aeruginosa 4 - C.b. The hybrid nano disinfectant showed the highest inhibitory activity against the two isolates A. baumannii (16 - A and 31 - A) with an inhibition diameter of 18 mm for each, while the highest inhibition activity for the free disinfectant was against the isolate A. baumannii 22 - B with an inhibition diameter of 19.5 mm.c. The hybrid nano disinfectant showed the highest inhibitory activity against the isolate K. pneumoniae (16 - B) with an inhibition diameter of 24.5 mm, while the highest inhibition activity for the free disinfectant was against the isolate K. pneumoniae 15 - B with an inhibition diameter of 24.5 mm also.d. The highest inhibition activity for the hybrid nanoic disinfectant against each of bacteria P. mirabilis and E. coli with an inhibition diameter of 23.5 mm, while the highest inhibitory activity for the free disinfectant was against the bacteria E. coli with an inhibition diameter of 24.5 mm, while the bacteria C. meningosepticum was the least affected among the other types of the isolated bacteria with an inhibition diameter of 8 mm and it was resistance to the hybrid nano disinfectant.e. The highest inhibition activity for the hybrid nano chlorhexidine was against the isolate S. aureus 21 with an inhibition diameter of 28.5 mm, while the highest inhibition activity for the free chlorhexidine was against the isolate S. aureus 22 - A with aninhibition diameter of 31.5 mm.f. The inhibitory activity of both the hybrid nano and the free disinfectant against C. guilliermondii yeast was closely to each other with an inhibition diameter of (28 and 29) mm, respectively.4. The study of antioxidant activity confirmed the following : a. Using free radical scavenging method, the IC50(Half maximal inhibitory concentration) value against ABTS free radical were at 10 µg /ml for free tannic acid while it was at 125 µg /ml for the hybrid nano tannic acid Mg/Al - TA - LDH.b. Measurement of ferric ion reducing power revealed that free tannic acid had reducing power greater than that of the hybrid nano tannic acid

التوصيف الجزيئي لبعض مسببات الاخماج الجلدية في محافظة الديوانية وتاثير جزيئات الفضة النانوية في نموها == Molecular Characterization of Some Dermatophytic Fungi In Al - Diwania Province And The Effect of Silver Nano Particles On Their Growth

Author name: نور جاسم محمد

Supervisor name: نيران عبيد جاسم

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Al-Qadisiyah - College Of Science - Department Of Biology

Language: Arabic

Full text from the source: https://qu.edu.iq/repository/wp-content/uploads/2017/04/%D9%85%D8%B1%D8%A9-%D8%B1%D8%A7%D9%81%D8%AF.pdf

University location: Qadisiyah

First pages:

Abstract: اجريت هذه الدراسة للتحري عن الفطريات المسببة للامراض الجلدية في الانسان حيث تم جمع 100عينة من الاشخاص المشكوك باصابتهم بالفطريات الجلدية من المرضى المراجعين لمستشفى الديوانية التعليمي من بداية تشرين الاول 2013 وحتى نهاية مايس 2014 وكانت هذه النماذج ماخوذة | This study was conducted to investigate the fungi that causes skin diseases of human. One hundred samples were collected from people who suspected to infect with fungal skin disease from patients coming to Al - Diwaniya teaching hospital from beginning of October 2013 till the end of May 2014. These samples from (skin, hair and nail). All samples that taken from patient were subjected to direct examinations by using wet touch of (KOH 10%) solution. At the same time the samples were grew on sabroud media to determine the type of fungal skin diseases of human. The direct examination shows positive results of 72 samples which represent 72% while for laboratory growth results, its shows 65 samples with positive results 65%. There are three types of Trichophyton fungus had been isolated which are T.rubrum 28 isolates, T.mentagrophytes 16 isolates and T.schoenleinii 4 isolates and there was one type of genus Microsporum which is M.canis 24 isolates. The results show that Tinea corporis infection is the common and its more among females than males where it is 26.39% and 13.89% respectively, then Tinea capitis which is 19.4% in males which is higher than in females which represents 13.89% while Tinea unguium was in females more than males which was in females 16.67% while in males 9.72%. The percentage of fungal skin infection was differ according to the location where the number of infections in the rural area for all types of Tinea were more than the number of infection in the urban area where the number of infected people with Tinea corporis was 18 which represent 25% while in the urban the number was 11 which represent 18.28% while the number of infected people with Tinea capitis in the rural area were 16 which represent 22.22% and the infected in the urban were 8 which represent 11.11%. While Tinea unguium the number of infected people in the rural 10 which represent 13.89% and in the urban 9 people which represent 12.5%. The diagnosis of isolated fungi depended on phenotype of fungal growth and microscopic examination, in addition to special tests for diagnosing skin fungal infections. Also the PCR was used for diagnosis and the work had been completed by using genotypic tree for fine diagnosis and detection the fungal species. The result were send to the genes bank site to be sure about the fungus type through comparison with the registered fungi in this site and by using MEGA6 program for genetic tree analysis of type (unweighted pair group method with arithmetic mean). The results of analysis shows clear converge of local Trichophyton spp. Isolates with Trichophyton rubrum in comparison with other types that appears in the genetic tree analysis while Microsporium diagnosed through genetic tree analysis of type (up GMA tree) and the result of analysis shows clear converge of local Microsporium spp. Isolates with Microsporium canis compared with other types of genetic tree analysis. The effect of silver nanoparticles that is used against growth of T.rubrum, T.mentagrophytes and M.canis. The results show that all the concentrations used were effective in stopping of growth of T.mentagrophytes when deal with the standard measure when dealt with concentration of 4 mg/l the more effective in stopping of radiating growth of the fungus. Regarding the fungus T.rubrum the concentrations of 4 mg/l more effective in stopping of growth while there was no difference for 1mg/liter concentration. Regarding the fungus M.canis, the mentioned concentrations had large effect on fungal colonization growth compared with the standard and this fungus more than other fungi affected by nano silver ions

دراسة تاثير المستخلص المائي لنبات اللهانة على مستويات هرمونات الدرقية, الاجهاد التاكسدي وعدد من المعايير البيولوجية في الارانب == Study The Effect of Cabbage Aqueous Extract On The Thyroid Hormones Levels, Oxidative Stress And Some Biological Parameters In Rabbits

Author name: سرى سمير محمد الدوري

Supervisor name: زيد محمد مبارك المهداوي | صاحب جمعة عبد الرحمن

General topic: Biology

Specific topic: Zoology

Degree: Master

University: Tikrit University - College Of Science - Department Of Biology

Language: Arabic

University location: Salahaddin

First pages:

Abstract: هدفت الدراسة الحالية الى معرفة بعض تاثيرات المستخلص المائي لنبات اللهانة ((Brassica oleracea على مستويات هرمونات الغدة الدرقية الثايرونين ثلاثي اليود Triiodothyronine (T3) والثايروكسين (Thyroxine (T4والهرمون المحفز للدرقية Thyroid stimulating hormone (TSH | The aim of the present study was to investigate some effects of the aqueous extract of cabbage (Brassica oleracea L) on the Thyroid hormones concentrations Triiodothyronine T3, Thyroxine T4 and Thyroid stimulating hormone (TSH), Body weight rate, and some physiological and biochemical parameters such as the total count of leukocytes (WBCs), packed cell volume (PCV) and concentrations of Hemoglobin (Hb), Glucose, Total Cholesterol(TC), Triglycerides (TG), High Density Lipoprotein (HDL - C), Low Density Lipoprotein (LDL - C), Very Low Density Lipoprotein (VLDL - C), Phospholipids, Atherogenic indices levels, Uric acid, Total protein, Albumin, Globulin, Serum Creatinine Blood urea, glutathione(GSH), Malondialdehyde(MDA), Peroxynitrite radical(ONOO - ) Ceruloplasmin(Cp), Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) in sera of male rabbits exposed to oxidative stress induced by hydrogen peroxide (0.5%) with drinking water along the period of experiment (30) days, with comparison the effect of the cabbage extract with the effect of carbimazole (anti - thyroid drug) to induce Hypothyroidism.For the purpose of this study, preliminary experiments were carried out to find the most effective dose of the cabbage extract and this dose was (300 mg/kg body weight).Rabbits (8 - 10 months age) and (950 - 1560 gm weight) were determined randomly divided into five groups, each group includes five replicates These groups are : The control group, cabbage aqueous extract group, cabbage aqueous extract and H2O2 group, carbimazole drug group and H2O2 GroupThe results showed the following : 1 - Treatment the animals with cabbage aqueous extract caused significant decrease (P<0.05) in Hb, PCV and concentrations of T3 hormone, T4 hormone, TC, LDL - C, phospholipids, second atherogenic index, blood urea, serum creatinine, albumin, MDA, (ONOO - ) radical and Cp, While it caused significant increase in weights, TSH hormone, TG, VLDL - C, glucose, globulin and GSH compared with the control group. While no significant variations were observed in total (WBCs) and concentrations of AST, ALT, HDL - C, atherogenic index (first and third), uric acid and total protein compared with the control group. 2 - Treatment of rabbits with carbimazole drug showed significant decrease in levels of body weight, Hb, PCV, and concentrations of T3 hormone, HDL - C, blood urea, albumin, glucose, GSH and Cp. This treatment showed as well a significant increase in T4 hormone, TSH hormone, ALT, TG, uric acid, VLDL - C, serum creatinine and atherogenic indices levels, Whereas showed no significant variations in total (WBCs), AST, TC, LDL - C, phospholipids, total protein, globulin, MDA and (ONOO - ) radical compared with the control group. 3 - Compared with the control group the oxidative stress induced by H2O2 caused significant decrease in concentrations of Hb, T3 hormone, HDL - C, albumin, GSH and Cp, While this stress showed a significant increase in total (WBCs), T4 hormone, ALT, TG, VLDL - C, phospho - lipids, atherogenic indices levels, serum creatinine, glucose, MDA and (ONOO - ) radical. However no significant variations were observed in weights, TSH hormone, PCV, AST, TC, LDL - C, uric acid, blood urea, total protein and globulin compared with the control group.4 - The Treatment of rabbits under oxidative stress with cabbage extract showed significant decrease in Hb, PCV, T3 hormone, TC, LDL - C, phospholipids, atherogenic indices levels, blood urea, serum creatinine, albumin, GSH, MDA, (ONOO - ) radical and Cp comparison with the control group. While a significant increase was observed in weights and concentrations of T4 hormone, TG, HDL - C, VLDL - C, uric acid, glucose and globulin, While no significant variations showed in total (WBCs), TSH hormone, ALT, AST and total protein comparison with the control group.The study results revealed that the effect of cabbage aqueous extract was better than carbimazole effect as antithyroid and as antioxidant role by reduction the harmful effects of the free radicals through some improvement was observed in many physiological and biochemical activities in the body and may be used in protection and treatment many of pathological cases specially Hyperthyroidism, cancer and cardiovascular diseases.