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تاثير الاشعة فوق البنفسجية Ultraviolet (UV) على بعض المعايير التكاثرية (معامل الانقسام للحيامن والتشوهات في رؤوس النطف) في الجرذان البيض == Effect of Ultraviolet On Some Reproduction Parameters (Mitotic Index of Sperms And Head Sperms Aberration)

Author name: قيس هادي لايذ الكرعاوي
Supervisor name: حسين خضير الميالي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Higher Diploma
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: قسمت 20 جرذ ابيض من الضرب Balb/C الى اربع مجاميع بعد تشعيعها (تشعيع الجسم كله) بفترات تعريض للاشعة فوق البنفسجية Ultraviolet لمدة (0 و20 و30 و60) دقيقة، ثم شرحت بعد 7 ايام من التعرض للاشعاع وتم حساب معامل انقسام mitotic index الخلايا الجنسية بالاضافة الى | Twenty white rats (Balb / C) have been divided into four groups after their exposure to ultraviolet ray for the following time periods (0, 20, 30, 60) minutes. Then the autopsy has been performed after 7 days of (UV) exposure and the mitotic index of sexual cell division has been counted. as well as the Head sperms aberration. The results of the study showed the following : 1 - Decrease in the mitotic index for the sexual cell. This decrease is directly proportional with the duration of exposure to radiation.2 - A slight increase in the percentage of the Head sperms aberration between (T 20) in comparison with the control group which is not exposed to radiation (T 0).3 - The increase in percentage of the Head sperms aberration is due to the (UV) and this abnormalities are directly proportional with the length of the exposure period as (T 30, T 60).

تاثير مستخلص الثوم والرمان على الجرذان المصابة تجريبيا بطفيلي Cryptosporidium parvum ومقارنتها مع عقار Metranidazole == Effect Extract Allium Sativum And Punica Grantium On White Rat Expermental Infection Cryptosporidium Parvum And Comper With Metranidazole

Author name: سرى رزاق خضير عبادة
Supervisor name: نجم عبد الواحد عبد الخضر الجدوع
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: جمعت عينات البراز من الاطفال في مستشفى النسائية والاطفال كذلك جمعت من العجول المصابة حيث اجري فحص العينات بصبغة الزيل نلسن ثم عزلت ونقيت الاكياس بطريقة التطويف بمحلول شيذر السكري وجرعت 2103xكيس بيض لكل جرذ بانبوبة التجريع الفموي وسجلت المدة قبل البائنة من | Collects stool samples from children in the women's and pediatric hospital. also collects all those from infected calves, where he conducts testing samples dye Zail Nelson then isolated and scrubbed bags manner floation solution Shidhir diabetes and gived rat 2 x103 bag eggs per rat tube dosage oral and recorded for the period before dowry of 3 - 5 days as recorded wounding 100%. The result is the appointment of the lethal dose of moderation LD50 of aqueous extracts of the garlic, peel the pomegranate after giving the rats doses graded by the mouth and the results indicate that the dose 7500 mg / kg of body weight is the lethal dose of aqueous extracts of garlic bulbs and dose limits of 5250 mg / kg is the lethal dose to peel pomegranate then tretment using cold aqueous extract to plant garlic. Allium satvuim L. peel and pomegranate Punica granantum L and drug Metronidazole (Flagyl) concentration of 250, 500, 750 mg / kg, the results shows the effectiveness of the current study, the aqueous extracts in the treatment of the parasites it turns out that the time required to kill the parasite suit inversely with the increasing concentration of extracts also show the efficiency of the aqueous extract of garlic bulbs and metronidazole concentration of 750 mg / kg in the treatment of 100% after 13 days of treatment with stops put bags of egg either concentration of 500 mg / kg of aqueous extracts garlic bulbs and metronidazole has shown the efficiency of treatment of by 100 % after 15 days of treatment showed, while the aqueous extract of pomegranate peel concentration 750 mg/kg therapeutic efficiency of 98.41% after 15 days of treatment, while the control group continued to launch until after the egg sacks (15) of treatment. The study shows histopathological section on the small intestine (duodenam, ileum, jejunum) Rats infection parasite get pathological changes represented by the crash of the villi of the small intestine and change epithelium from the vertical to the cube and the adhesion of some of the villi and gatherings of inflammatory cells in layer plate fitted, as well as for necrosis and desquamation of the cells lining the villi also destroyed gland and appeared egg sacks in the intestine result use 2x103 frome egg sacks also appeared effective abstract aqueous garlic in the intestinal tissue to restore its natural form after 15 day use concentration 750 mg/kg of aqueous extract of garlic bulbs and metronidazole while have taken normal sat to tissue treaement with aqueous pomegranate peel long than 15 day. test the effectiveness of aqueous extracts cold of garlic bulbs. Alluium sativum L. and peel pomegranate Punica granantum L in treatment parasite spores Cryptosporidum parvum and compared with the treatment of Metronidazole (Flagyl) in laboratory rats. Conducted on plant extracts cold of garlic and pomagranite the chemical tests have shown the presence of active substances of both alkaloids, carbohydrates, Flavonides, Alglycosidat, Alsaponyat, Alresins, phenolies

تاثير بعض العوامل البيئية والمايكروبايولوجية في تنقية مياه الشرب لمحطة المجمع السكني في قضاء الدور - محافظة صلاح الدين == Effect of Some Ecological And Microbiological Factors On Drinking Water Purification Plants In Al - Mugama Al - Sakany In Al - Dour City - Salahaldin

Author name: فخر الدين نور الدين رشيد الخفاف
Supervisor name: حميد سلمان خميس المهداوي
General topic: Biology
Specific topic: Aquatic Environment
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تضمنت الدراسة جمع عينات شهرية من مياه نهر دجلة ومحطة مياه الشرب العائدة للمجمع السكني في الدور اخذت العينات من خمس محطات مختارة ابتداء من شهر تشرين الثاني 2007 ولغاية شهر تشرين الاول 2008 لغرض دراسة بعض العوامل الكيمياوية والفيزياوية والبايولوجية السائدة | This study was included collecting of monthly water samples from Tigris river and water purification plant in AL - Mugama AL - Sakany in AL - Dour, These samples were collected from five chosen locations, started from November 2007 until October 2008, To Study The dominance of some chemical, physical and Biological factors Through the year its effects on water purification plants in AL - Mugama AL - Sakany in AL - Dour. These factors included air and water temperature, Turbidity, Electrical conductivity, pH, dissolved Oxygen, BOD, Total alkalinity, Total hardness, Calcium and Magnesium hardness , Chloride reactive nitrite, nitrate phosphate and salinity as well as some Biological factors such as Total number of bacteria and diatoms during the study period. The results of this study shown that there was a seasonal variation in some chemical and physical factors. where the value of air temperature ranged between (3.5 - 40) C and water temperature ranged between (3.4 - 32.5) C.Turbidity value ranged from (2.2 - 22.94) NTU while electrical conductivity ranged between (320 - 590) µs / cm. The results also showed that the water in Tigris river was alkaline, where the pH value ranged between (7.08 - 7.98). and aeration. The avarices value of dissolved oxygen ranged between (3.5 - 10.2) mg/L and The BOD value never increase to critical value, its ranged between (1.58 - 2.37) mg /L salinity ranged between (0.110 - 0.305) mg/L while chloride concentration ranged between (21.78 - 23.82) mg / L. It has been found that total hardness ranged between (170 - 370) mg/L while total alkalinity for the five station ranged between (124.1 - 137.8) mg No2/ L while Micronutrients ranged from (0.388 - 0.449) mg / L and the value of nitrate ranged between (ND - 0. 598) mg No2/ L and (0. 65 - 122) mg Po4 / L. In General where we compare the monthly average for physical and chemical variation for Tigris water with standard world limits applied for grouping of surface water, we can consider the quality of Tigris water is good and suitable for uses as a source for drinking water by using traditional purification of water.While the results of study of Biological factors showed that the total number of bacteria for Tigris water was modularity clear and unsafe for drinking with out treatment include sterilization. High number of bacteria was found in station (1) during February 2008 when it reached (284) cell / ml and the lowest average recorded in station (5) at December 2007 when its reached (50) cell/ml. This study also shown that high number of diatoms were recorded in station (1) when its reached (46860) cell/L at December 2007 and the lowest number recorded in station (5) when it reached (236) cell / L at November 2007. It has been identifies (11) genus and sp. of diatom in this stations and there were :

تحديد مقاومة بعض الجراثيم المعزولة من اخماج في مستشفى تكريت التعليمي للمضادات الحيوية وعلاقتها بالبلازميدات == Determination The Antibiotic Resistance of The Some Isolated Bacteria From Tikrit Teaching Hospital Infections And Their Relationship With Plasmids

Author name: شيماء عبد محمد علي
Supervisor name: وعد محمود رؤوف | خالد عمر محمد علي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: جمعت (140) عينة من المرضى الراقدين لثلاثة ايام فاكثر في مستشفى تكريت التعليمي للمدة من 1 - 8 - 2009 ولغاية 1 - 12 - 2009 بهدف تحديد العزلات الجرثومية الاكثر شيوعا ضمن اخماج مستشفى تكريت التعليمي ومقاومة هذه العزلات للمضادات الحيوية ودراسة العلاقة بين مقاو | One hundred fourti samples were collected from hospitalized patients for three days or more in the Tikrit Teaching Hospital during the period from 1/8/2009 until 1/12/2009 to determine the most commonly isolated bacterial from nosocomial infections in Tikrit Teaching Hospital and their resistance to antibiotics and to study the relationship between antibiotics resistance and plasmid content in the most commonly isolated bacteria and to evaluate the prevalence of these plasmids in the hospital environment. These samples include : (75) Urine, (28) stool, (28) of wound swabs and (9) sputum samples.The incidence of nosocomial infection was (45.7%) of them, urinary tract infections were (50.7%) from (75) samples, followed by wound infections (42.9%) from (28) samples, gastrointestinal infections (39.3%) from (28) samples then pneumonia (33.3%) from (9) samples the nosocomial infections were more common in females and age group 51 - 89 years. Different bacterial species were isolated in this study and the results showed that the most commonly isolated type was Citrobacter koseri in a proporation (21%) of urinary tract infections, followed by the following bacterial species : Escherichia coli (15.7%), Staphylococcus schleiferi (10.5%) , Staphylococcus epidermidis (7, 8%), Proteus vulgaris, Staphylococcus saprophytica, Klebsiella ozaenae, Klebsiella ornithindytica in aproportion (5.2%) for each, Enterobacter aerogenes, Providencia stuartii, Staphylococcus warneri, Staphylococcus aureus, Proteus mirabilis in aproportion (2.6%) for each.Citro. koseri were also the most common in wound infections (25%), followed by the following bacterial species : E.coli (16.6%), P.mirabilis (16.6%), Staph.schleiferi, P.vulgaris, Prov.stuartii and Prov.rettgeri in aproportion (8.3%) for each. Citro.koseri were also the most common in the gastrointestinal tract infections in a proporation (36.3%), followed by E.coli (18.1%), S.flexneri (18.1%), P.mirabilisc, Prov.stuartii, Prov.alcalifaciens in a proporation (9%) for each. . While Strept.pneumoniae was the most common(66.7%) in penemonia cases followed by K.pneumoniae (33.3%).The isolated bacteria were tested for antibiotics susceptibility and it has been found that most of these isolates showed high resistance to the studied antibiotics other than Prov. Stuartti was sensitive to all antibiotics except of Rifampicin and Tetracycline. As well as bacterial species belonging to the genus Staphylococcus were sensitive to Amikacin, Staph.aureus was also sensitive to Nitrofurantoin while Staph.warneri was also sensitive to Ciprofloxacin and Nitrofurantoin also Enterobacter aerogenes was resistant to all antibiotics except for Ciprofloxacin and Nitrofuratoin. Eleven of most commonly isolated bacteria which were multidrug resistant were chosen for the curing experiments using SDS to study the relationship of their plasmids with resistant to antibiotics. The results showed that the cured cells of E.coli lost their resistance to Tetracycline Ciprofloxacin and Cefotaxime which Citro.koseri lost their resistance to Tetracycline while K. ornithinolytica lost resistance to both antibiotics Tetracycline and Ciprofloxacin either P.mirabilis lost resistance only to tetracyclin as well as Strept.pneumoniae lost their resistance to Cefotaxime, tracycline and Ampicillin. Which may mean that the genes responsible for resistance to these antibiotics are located on plasmids as proven by the results of electrophoresis.Electrophoresis results also showed that all studied bacterial isolates had the same plasmid profile which may regarded as an epidemiological pointer that plasmids are conjugative which have the ability to transfer antibiotic resistance to other bacterial strains of the same species or to other different bacterial species.

تقييم بعض المؤشرات المـناعية للتفاعلات الالتهابيـة المتـرافقـة مـع الاصابة بطفيلي الاكياس المائية == Evaluation of Some Immunological Parameters Associated With Echinococcus Grunulosus Infection

Author name: حارث برع حسن علي الاوسي
Supervisor name: نغم ياسين كاظم البياتي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: اجريت الدراسة الحالية في محافظة ديالى وشملت الدراسة 184 عينة من الاشخاص الاكثر تعرضا للاصابة بمرض الاكياس المائية. وبعد الكشف تبين ان 16 منهم تاكدت اصابتهم بالاكياس المائية في كل من الكبد والرئتين (12, 4 مريضا على التوالي) وقد تم تسجيل بعض المعلومات من كل | The present study was carried out in Diyala province, and included 184 samples at risk of infection with hydatid cysts. The diagnosis obtained that there were 16 infectied individual with hydatid cysts in liver and lung (12, 4 patients, respectively). Some information were recorded for each individuals. The study included 24 healthy individuals as control. Immunological test was carried out for each group. The results obtained that the rate of infection was higher in female (4.9%) compared with male (3.8%). The highest rate of infection was in 31 - 40 and 41 - 50 age groups and there was no significant difference among age groups. According to occupation there was no significant difference and the student shepherds were not infected with hydatid cyst. When investigation of risk factor, the result showed that (3.26%) of infected individuals were contact with animals while those who eat vegetables were higher (5.43%). The liver was the first infected organ according to distribution of infection in body follow by lung with 75% and 25% respectively and there was no significant difference. According to cytokines, IL - 4, IL - 17A, MIP - 1?, IP - 10, the study were higher in infected individuals compared with non infected and there was significant difference at 0.001 p.value. There was no significant difference in means of IL - 4 and MIP - 1? in both sexes but there was significant difference in IL - 17A and IP - 10 between males and females. The study showed that there was significant difference in IL - 4 according to location of infection and it was higher in lung compared with liver. No significant difference in IL - 17A, MIP - 1?, IP - 10 was shown according to location of infection

تقييم نوعية مياه نهر الفرات (بين مدينتي الكفل والشنافية) وسط العراق == Assessment of Water Quality of Euphrates River (Between The Cities of Al - Kifl And Al - Shinafiyah) Iraq

Author name: دنيا باهل جدعان صلال الغانمي
Supervisor name: حسين يوسف خلف الركابي
General topic: Biology
Specific topic: Aquatic Environment
Degree: Doctorate
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: اجريت الدراسة الحالية على جزء من بيئة نهر الفرات الممتد بين مدينتي الكفل والشنافية لمدة عام تقويمي من ايار 2013 ولغاية نيسان 2014 واختيرت اربعة مواقع مختلفة وموزعة على منطقة الدراسة شملت ثلاث محافظات هي : بابل - النجف - القادسية (الفرات الاوسط - العراق)، | The current study has applied on the Euphrates River for a full year from May 2013 until April 2014. Four sites selected along the river whithin three provinces; Babylon, Najaf, Al - Qadisiyah. Assessment of water quality done by using Canadian Council of Ministers of Environment (CCME) and Index of Biological Integrity (IBI) for the purpose of determining the validity and quality of its water for the General purpose and for Drinking and Irrigation.The physicochemical properties and biological (Phytoplankton) were studied during the study period. These properties were; temperature of the air and water, light penetration, electrical conductivity, salinity, turbidity, water current speed, total suspended and dissolved solids, dissolved oxygen, biochemical oxygen demand, chemical oxygen demand, carbon dioxide, total alkalinity, acidity, total hardness and ions of calcium, magnesium, sodium, potassium, and the sodium adsorption ratio, chlorides, sulfates, boron, nitrite, nitrate, phosphate, silica and some heavy metals such as cadmium, lead, zinc, copper, chromium, manganese in both phases : dissolved and particulate. results showed that the values of the physical and chemical factors ranged as follows : (7 - 47) C, (10 - 31) C, (14 - 177.5) cm, (1020 - 5600) µs/cm, (0.65 - 3.46) 0%, (0.07 - 129) NTU, (27.03 - 115.57) cm/s, (591 - 2985) mg/L and (0.001 - 0.128) mg/L, (7.04 - 8.8), (6 - 11.8) mg/L, (0.13 - 6.6) mg/L, (8.2 - 53) mg/L, (81.4 - 198) mg/L, (30 - 200) mg/L, (6 - 46) mg/L, (490 - 929.67) mg/L, (83.37 - 290.18) mg/L, (0.79 - 174.84) mg/L, (92.5 - 713) mg/L, (1.9 - 11.18) meq/L, (6 - 133) mg/L, (97.97 - 861.73) mg/L, (329 - 1040) mg/L (0.01 - 4.23) mg/L, (0.008 - 1.33) µg/l, (2.16 - 44.74) µg/L, (0.012 - 0.59) µg/L and (104 - 514) µg/L respectively.Concerning the heavy elements, the values of dissolved cadmium ranged between (undetectable - 0.075 mg/L) and it's particulate between (0.0043 - 0.2705) mg /L and dissolved lead between (undetectable - 0.0232 mg /L) and it's particulate between (0.0045 - 0.148) mg/L and dissolved zinc between (un - detectable - 0.0168 mg/L) and it's particulate between (0.0115 - 0.316 mg/L) and dissolved copper (undetectable - 0.0273 mg/L) and copper is particulate between (0.0062 - 0.063 mg /L) and dissolved chromium between (undetectable - 0.0193 mg/L) and the chromium is particulate between (0.0043 - 0.0449) mg/L and dissolved manganese (undetectable - 0.0256) mg/L and it's particulate between (0.0085 - 0.0739) mg/L.The biological tests include Qualitative and Quantitative Study on phytoplankton and also included calculating the total number of coliform way casting dishes by using the MacConkey Agar media.The number of diagnosed species of Phytoplankton was 295 species, belonging to 74 genera. The Diatoms Bacillariophyceae won the biggest part of it as it formed 160 species and 35 genera any rate (54.24%), followed by green algae (70 species belonging to 24 genera) and green algae (52 species belonging to 11 genera) then Euglenophyceae (10 species belonging to both sexes), while golden brown algae and algae Pyrrophyceae were less algal groups registered as belonging to the two single - sex. Some types of phytoplankton existed in most of the duration of the study, including Bacillaria paxillifera, Cocconies placentula, Cyclotella meneghiniana, Diatoma elongatum, Fragilaria virescence, Melosira ambigua, Synedra acus.The total number of phytoplankton is estimated between (104.9 - 4460.09) cell × 103/liter. An increase in the number of phytoplankton in July and January was observed clearly. The concentrations of chlorophyll - a were ranged between (0.0693 - 9.78 mg /L). While the total number of results for coliform were (5 - 281 × 103) cells/liter, The water quality of the evidence Account (Canadian model) using a number of physical, chemical and Biological properties. The results showed that the waters of the Euphrates are located within categories (Poor - Good) with respect to the public evidence of the quality of water of this river while it was located within categories (Poor - Acceptable) with respect to their use for drinking, While its use for irrigation purposes, it was clear that run river water data fall into two categories (Poor - Good). The results of the analysis of the main components Principal component Analysis (PCA) indicate that the nitrates and the total number of coliform and dissolved oxygen, phosphate, zinc, lead and Biological oxygen demand and pH are the biggest impact on the water quality of the index values of the variables of general purpose.These factors are responsible for the deviation index values, followed by turbidity and total hardness and electrical conductivity and temperature of the water, copper, chromium, and cadmium heat. But with the biggest impact of the index water quality factors for drinking purposes are chloride and electrical conductivity and total hardness, magnesium, turbidity and basal college, sodium, calcium, copper, sulfates, nitrates and coliform, zinc and dissolved oxygen, pH, manganese and lead followed by boron nitrite, cadmium, chromium and vital requirement for oxygen. While the chloride and conductivity electrical, the proportion of sodium, copper, zinc and adsorption of boron have greatest influence on the value of water quality index for irrigation, followed by manganese, cadmium, chromium, lead and pH.The phytoplankton index of Biotic Integrity (P - IBI) has been calculated using ten units including (relative abundance of green algae, green algae, pyrrophyta, Euglenophyta, Xanthophyta, Centralales, pennales, species richness index, the density of phytoplankton and chlorophyll a) The results showed that phytoplankton index of Biotic Integrity values ranging between (47 - 74) in the first position and (47 - 67) in the second location, and (45 - 76) in the third site and (45 - 71) in the fourth site. In other words, phytoplankton index of Biotic Integrity with estimating index (good - poor). The results showed the effectiveness of phytoplankton index of Biotic Integrity values in estimating the environmental changes.

التحديد الجزيئي لبعض عوامل الضراوة في بكتريا Klebsiella spp المعزولة من مصادر سريرية مختلفة == Molecular Identification of Some Virulence Factors In The Bacterium Klebsiella Spp Isolated From Different Clinical Sources

Author name: فرقد حسن فليح
Supervisor name: بهيجة عبيس حمود الخالدي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: جمعت (150) عينة من المرضى المراجعين والراقدين في مستشفى الديوانية التعليمي والذين يعانون من اصابات مختلفة وللفترة من 1/كانون الاول/2013 ولغاية 1/نيسان /2014 وتضمنت هذه العينات ماياتي : (50) عينة حروق وجروح، (50) عينة ادرار و(50)عينة قشع من المرضى المراجعين | One hundred fifty samples were collected from patients admitted in Al - Diwaniyah Teaching Hospital and suffering from various injuries and for the period from 1 / December / 2013 and up to 1 / April / 2014 these samples in clude : (50) sample burns and wounds, (50) urine and (50) sputum.The results showed that 90 samples (60%) of the total number gave is positive result for the growth.the bacterial isolates were distributed among 35 samples (70%) of burns and wounds, (33) the sample (66%) from urine and (22) sample (44%) from sputum After biochemical and cultural test found that 99 isolate belonging to Different Genus and species of bacteria these isolates include 40 isolate (44.44%) bound and wound isolates Staphylococcus sp was the predoniant in these samples by rate (42.5%), 36 isolate(40%) E.coli was most isolate by rate 50% in urine sample and 23 (25.5%) Klebsiella pneumonia was the most common isolate by rate (78.26%) in these putum.. Sensitivity test results of antibiotics showed that most of the isolates have high resistance against antibiotics used in the study and the most isolates possess multiple resistance (Cross resistance) as shown resistance to most of antibiotics that used in these study. All asolates (% 100) were resistant to nitrofurantin and Tetracycline while(95%)is resistant to ampiciline (90%) of isolate were resistant to thired generation of cephalosporins (ciprofloxacin and ceftazidime) and (%80) of isolate wereresistant to gentamicin and impeneme, finely (%80)of isolate were resistant to azetroname..The results of the molecular detection of genes uge and mag A gene in Klebsiella sp in the 16 (50%) of the isolates had mag A as the 8 (44.44%) of the isolates bacteria K.pneumonia carrier of mag A and 8 isolates of type K.oxytoca had of the gene above the gene uge was his appearance in the proportion of isolates (46.87%) as it appeared in 9 (50%) of the isolates K.pneumonia and 6 isolates (42.87%) of the isolates k.oxytoca..There were relationship between resistance of bacteria to antibiotics and possession of these genes

دراسة تشريحية لسيقان وسويقات واوراق بعض اجناس العائلة الوردية rosaceae في الديوانية == Anatomical Study of The Stems, Leaves And Petioles For Some Species of Family Rosaceae

Author name: حسنى مهدي رياح الفتلي
Supervisor name: ازهار عبد الامير سوسة
General topic: Biology
Specific topic: Plant
Degree: Higher Diploma
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: شمل البحث الحالي دراسة بعض الانواع العائدة للعائلة الوردية Rosaceae في محافظة الديوانية من الناحية التشريحية, اذ تم دراسة المقاطع المستعرضة للسيقان والسويقات والاوراق لثلاثــــــــــــة انــــواع وهـي المشمـــــش Prunus armenicana L. والكمثـــــــــري.Pyr | Current research involved the study of some species belonging to the family rosary rosaceae in Diwaniyah province of anatomical terms, as has been the study of cross sections of the stems, leaves and petioles for three species apricot Prunus. armenicana L. And pear Pyrus communis L. And apple Malus domestica Borkh. The study has been the separation of these species into groups depending on differences and similarities of the attributes of the obvious anatomical changes were discussed among these species, It turns out that many of these qualities, Especially the form of stem and cutworms as well as the presence or loss of tissue such as textiles Parencyma green Chlorenchyma in the stem as possible discrimination species P.armenicana for the other two species of loss of such a fabric, And also the possible discrimination of this species through the general shape of the clips transverse Soik as it's looks like a horse shoe, While the other two it's like a triangle. As for the indumentum and taxonomic diversity epithet to isolate and diagnose these species from each other, Such as Glandular hairs and the loss of these filaments in the other two species.The study concluded that the possibility of the use of the anatomical characteristics of transverse sections of the Stem, Petioles and Leaves in the diagnosis of the species under study

تاثير الاصابات المجهرية المهبلية وبعض الهرمونات على الاصابة بالمشعرات المهبلية عند النساء في بعقوبة - محافظة ديالى == Effect of Microbial Vaginal Infections And Some Hormones On Trichomonas Vaginalis Infection In Women In Baquba - Diyala Province

Author name: حنان رحيم حسوني الخشالي
Supervisor name: نغم ياسين كاظم البياتي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: : توجد هناك عدة عوامل تلعب دور مهم في زيادة الاصابة بطفيلي المشعرات المهبلية وهذه العوامل تتضمن : اختلال مســـــتوى الهرمونات الجنســـــية, تغير الرقم الهيدروجيني للمهبل, بعض انواع الاصابات البكتيرية.الهدف من الدراسة : اولا : تحديد نسب وانواع الاصابات ال | Introduction : It have been founded that many factors play important roles in the increase incidence of infection with Trichomonas vaginalis these factors includes : disturbances in sex hormones level, change in pH of vagina and some types of bacterial infections.Aims : First : To determine the rates and types of Microbial vaginal infections that infected women in Baquba - Diyala province.Second : study of the relationship between the Trichomonas vaginalis and other microorganisms.Third : study the relationship between the Trichomonas vaginalis and the levels of some sex hormones.Patient and Methods : This study was carried out on 227 patients attended either Al - Batol Teaching Hospital for Maternity and Children in Baquba - Diyala, or some private clinic in Baquba city, most of these patient giving symptoms of vaginitis as a patient group, in addition to 10 healthy women as a control group.Patients group included married, widows and divorcees women with age range between (18 - 45) years, and at different educational level.Full clinical evaluation was done for those patient including history and physical examination. This evaluation revealed that 209 of patients (92%) had vaginal discharge, irritation and itching. Reguested blood samples were taken for laboratory test included estradiol, progesterone and testosterone. The level of these sex hormones in women with Trichomoniasis have been determined and compared with levels of these hormones in control group.In addition high vaginal swab were taken for microbiological tests. and used for diagnosis of Trichomonas vaginalis and other associated microorganisms, the rate of parasitic infection had been determined and it's relation with age group, marital status, level of education and level of vaginal acidity had been estimated. Moreover, the percentage of cases, with pus cells, epithelial cells, red blood cells and clue cells have been estimated. Results : the results of laboratory tests elicited the presence of Trichomonas vaginalis (6.3%). And in addition to it there is bacterial and fungal vaginal infection and the percentages were (54%) and (15%), respectively. Moreover, the results showed the presence of dual infections between the parasite and bacteria, the parasite and fungi, and bacteria and fungi and there percentages were (11.8 %), (8 %) and (4.9 %), respectively.Regarding age group, results showed that the highest parasite infection rate was among women in the age group (26 - 35 years) which was (41.1%), in compare with infection rate in others age group (18 - 25 years) and (36 - 45 years) which were (9.3 %) and (18 %), respectively. Regarding, the marited status, the highest infection rate was founded among married women in comparison with widows, divorcees women, and the percentages were (31.7 %), (6.8 %) and (14.7 %), respectively. Regarding, the educational level, the results showed highest rate of infection with Trichomonas vaginalis was among women with low educational level which was (11.8%) in compared with those with intermediate (7.9%), secondary (4.8%), and tertiary education (1.3%). Regarding, the pH of the vagina, results showed that the rate of vaginal infection were high when pH of vagina > 4.5 in comparison with the lowpH ? 4.5 and the percentages were (83.7%) and (16.3%), respectively. The results of direct smear and cultures showed that (25.9 %) of case (59 patient) were infected with Trichomonas vaginalis, (31.7%) of case infected with E.coli, (27.7%) of case infected with candida albicans, (21.1%) infected with Gardnerella vaginalis, (17.6%) percentages of Lactobacillus spp. (8.3%) percentages of Staphylococcus epidermidas (5.2%) in fected with S. aureus, (3.5%) infected with Enterobacter spp., (2.6%) infected with Proteus spp., (2.2%) infected with Klebsiella spp., (1.7%) infected with Acentobacter spp. and Streptococcus spp., (1.3%) infected with Pseudomonas spp., While the lowest rate was for Neisseria gonorrheae (0.8%). Also results showed that the highest percentage of dual infection with trichomoniasis it was in Gardnerella vaginalis which detected in 22 case (37.2%), followed by Candida albicans, which detected in 18 case (30.5%). While the lowest rate was with Neisseria gonorrheae which detected in one case (1.6%). The other laboratory tests including hormonal estimation in patients with trichomoniasis, the results of current study showed decrease level of estradiol hormone in women infected during follicular and luteal phases of the menstrual cycle, In follicular phase the mean of estradiol was (6.83 pg/ml) in comparison with control group that hormone rate was (42.61 pg/ml). In luteal phase the mean of estradiol was (27.00 pg/ml) comparison with control group which hormone rate was (87.16 pg/ml). In addition to that the level of progesterone have increased in follicular phase but decreased in luteal phase in comparison with control group. In follicular phase the mean of progesterone was (5.89 ng/ml) in com - parison with control group which hormone rate was (0.72 ng/ml). In luteal phase the mean of progesterone in infected women was (0.56 ng/ml) comparison with control group in which hormone rate was (19.83 ng/ml).Regarding, the level of testosterone, the results showed that the level had been increased during the follicular and luteal phases in infected women compared with control group. In follicular phase the mean of testosterone was (3.44 ng/ml) in comparison with control group which the mean was (0.24 ng/ml). In luteal phase the mean of testosterone was (3.65 ng/ml) in comparison with control group in which the mean was (0.30 ng/ml).Conclusion : The presence of infection with Trichomonas vaginalis, and it's associated with other microorganisms that are detected in the current study and other study previous may indicated a correlation between them. So those patient, constantly in need for a panel of laboratory test including : vaginal pH, and hormonal estimation as these factors may play a role in diagnosis and follow - up of the patients.

تاثير دوالي الخصيتين في معالم المني وسلامة الحامض النووي الـDNA == Effect of Varicocele In Semen Quality And Nucleic Acid DNA Integrity

Author name: حيدر لطيف فرحان المساعد
Supervisor name: صاحب يحيى حسن المرشدي | عبد الزهرة كاظم محمد علي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: شملت هذه الدراسة فحص 150 عينة من السائل المنوي لمرضى القيلة الدوالية Varicocele والاشخاص الخصبين بعد مدة امتناع قدرها 3 - 5 ايام حيث جمعت العينات في مختبرات مركز الخصوبة / مدينة الصدر الطبية / محافظة النجف الاشرف من 1 / 10 /2012 الى 30/ 4 /2013, لقلة الدر | This study was included 150 samples of semen for varicocele and fertile persons. The samples were collected after 3 - 5 day abstinence period at fertility center Laboratories / ALsader Medical city in An Najaf province.For the period from 1/10/2012 to 30/4/2013.Because the studies about varicocele are rare, the present studies aimed to know the impact of varicocele on semen and sperm parameter, sperm quality index, Malondialdehyde concentration and the percentage of sperm DNA integrity.So, this study was aimed at investigation wethere the Varicocelectomy in varicocele patients could positively influence studied parameters.The research was involved examining 83 sample of seminal fluid for varicocele patient. The percentage of varicocele grades were examined and the results were represented 40% grade1, 39% grade2 and 21% grade3.The result were showed a significant decrease (P<0.05) in sperm concentration , progressively motile sperm, normal sperm morphology percent and sperm viability percent for varicocele patient compared with the fertile persons, while there was noticed a significant increase (P<0.05) in Round cells concentration snd liquefaction time for varicocele patient compared to fertile person and the result of this study were showed significant decrease (P<0.05) of sperm quality index and sperm DNA integrity for varicocele patient compared to fertile persons (control).The study noted to the significant increase (P<0.05) of malondialdehyde concentration for varicocele patients compared to fertile persons. The coefficient factor was used to investigate the correlation between malondialdehyde concentration and the studies parameter.there was increase correlation (P<0.05) between malondialdehyde concentration and sperm concentration(r= - 0.86 P<0.05) progressively motile sperm (r= - 0.938), normal sperm morphology percent (r= - 0.90 P<0.05), sperm viability percent (r = - 0.846 P<0.05) and sperm quality index (r = - 0.836 P<0.05), while there was (P<0.05) positive correlation between malondialdehyde concentration and semen volume (r=+0.822 P<0.05), liquefaction time (r = +0.828 P<0.05) and round cells correlation (r= + 0.89 P<0.05).Also the coefficient was used to study the correlation between sperm DNA integrity and other study parameter. The results of this study were showed positive correlation between the percentage of DNA integrity and sperm concentration (r= +0.88 P<0.05), progressively motile sperm (r=+0.78P<0.05), normal sperm morphology (r= +0.86 P<0.05), sperm viability percentage (r=+0.87 P<0.05) and sperm quality index (r= +0.81 P<0.05), while it was noticed in used correlation between sperm DNA integrity and malondialdehyde concentration (r= - 0.681 P<0.05), semen volume (r= - 0.67 P<0.05), liquefaction time (r= - 0.65 P<0.05) and round cells (r= - 0.54 P<0.05). The effect of varicocele grading on sperm and semen parameter, sperm quality index, malondialdehyde concentration and percentage of sperm DNA integrity was studied, the result were showed a significant decrease (P<0.05)of sperm concentration, progressively motile sperm, sperm viability and normal sperm morphology percentage, and a significant increase (P<0.05) of liquefaction time and Round cells concentration for varicocele patients grad1, grade2 and grade3 compared with the fertile persons. and when we are compare between varicocele patient according to type of grade the results were show a significant decrease (P<0.05) of sperm concentration, progressively motile sperm and normal sperm morphology percentage and a significant increase (P<0.05) of liquefaction time for varicocele patients grade2 and grade3 compared to grade1 varicocele patient.So the result showed a significant decrease (P<0.05) of sperm concentration for grade3 varicocele patients compared with the grade2 varicocele patients. The result of this study were showed a significant decrease (P<0.05) in sperm quality index and sperm DNA integrity for grad1, grade2 and grade3 varicocele patients compared to fertile persons. also it was noticed a significant decrease (P<0.05) of sperm quality index and sperm DNA integrity for grade2 and grade3 varicocele patients compared to grade1 varicocele patients but the malondialdehyde concentration according to varicocele grade, the result were showed a significant increase (P<0.05) of malondialdehyde concentration for grade2 and grade3 varicocele patients compared to grade1 varicocele patients and fertile persons. In this study the effect of Varicocelectomy on study parameters was studied and the result were showed a significant increase (P<0.05) of sperm concentration, progressively motile sperm, sperm viability percentage, normal sperm morphology, percent, sperm quality index and sperm DNA integrity and a significant decrease (P<0.05) of liquefaction time and malondialdehyde concentration for Varicocelectomy compared to varicocele patients. The result were showed a significant decrease (P<0.05)of sperm viability percent, normal sperm morphology percent, sperm DNA integrity and sperm quality index and a significant increase (P<0.05) in round cells concentration, liquefaction time and malondialdehyde concentration for Varicocelectomy patients compared to fertile persons.From the result of present study, we are concluded that varicocele has been negatively impact on sperm and semen parameters, malondialdehyde concentration and sperm DNA integrity, and the effect of varicocele is associated with the increasing of varicocele grading, while Varicocelectomy has been positive impact on sperm and semen parameters, malondialdehyde concentration and sperm DNA integrity but this result have not reached to similar state to fertile persons

انتشار المقاومة للمضادات الحيوية وبعض عوامل الضراوة في بكتريا Pseudomonas aeruginosa المعزولة من مستشفيات في النجف المعزولة من مستشفيات في النجف == Molecular Study To Some of Pseudomonas Aeruginosa Virulence Factors Isolated From Clinical And Environmental Isolates

Author name: ذو الفقار حيدر حسن
Supervisor name: ماجدة مالك متعب
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: اجريت الدراسة الحالية لغرض التحري عن وجود الجينات الكروموسومية المسؤولة عن بعض عوامل الضراوة المهمة لجنس Pseudomonas aeruginosa كانتاجها للسم الخارجي A والالجنيت اضافة الى الجينات الكروموسومية المسببة لمقاومة الكوينولونات في العزلات السريرية والبيئية لبكت | This study include investigate the presence of chromosomal quinolone resistance genes and virulence genes(toxA and algD) in clinical and environmental isolates from Al - Sadr Medical city.A total of 110 samples were collected from different clinical sources(burns, wounds, and urine), and 50 from the hospital environment.the samples were collected during 10, Oct, 2012 to 2, Feb, 2013. A total number of Pseudomonas aeruginosa isolates accounted for 89 of 160 samples.The distribution of these isolates were 28 from Burns, 14 wounds, 15 urine, and 32 from hospital environments. Identification of isolates has been accomplished depending on phenotypic diagnostic methods and PCR technique.our study proved that PCR technique depending on oprI and oprL genes was a rapid method for identification of non - pigmented isolates. A total of 20 antibiotics resistant isolates were chosen for Molecular studies. The result reveling that 100% of isolates had a chromosomal mutation in the QRDRs of genes (gyrA, gyrB, parC, and parE). High percentage of isolates(45%) carried mutated genes gyrA and parC, followed by 15% of isolates were carried either mutated gene gyrA alone or three mutated genes gyrA, parC, and parE and and 10%of isolates were carried three muted genes gyrA, gyrB, and parC, while gene that encoding to parC mutation were detected in 5% of isolates. Our study is the first study about detection of Quinolone Resistance Determining Regions in P.aeruginosa in Iraq. The results showed the the presence of mexAB - oprM multidrugs efflux system expression in 100%(20) isolates. while the phenotypic test depending on MIC values for ciprofloxacin alone in the presence of efflux inhibitor showed that only 15%(3) isolates had the active efflux pump.

العلاقة بين كروماتين النطف ومعايير المني في مرضى عديمي الخصوبة == The Relationship Between Sperm Chromatin And Semen Parameters In Infertile Patients

Author name: وليد عبد علي حسن الخفاف
Supervisor name: صاحب يحيى حسن المرشدي | زينب شنيور مهدي الطرفي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: هدفت الدراسة الحالية الى تقييم النسبة المئوية لكروماتين النطفة غير السوي Abnormal sperm chromatin percentage لمرضى عديمي الخصوبة مقارنة بالرجال الخصبين ودراسة العلاقة بين النسبة المئوية لكروماتين النطفة غير السوي ومعايير المني ومؤشر نوعية النطف وتركيز الم | The aim of the current study was to evaluate the Abnormal sperm chromatin percent for the infertile comparing with the fertile men and study the relation between the Abnormal sperm chromatin percent, the sperm and semen parameters, sperm quality index and MDA concentration for the infertile normozoospermic and asthenozoospermic patients comparing with the fertile men.This study was conducted in the laboratories of fertility centers in Al - Sader medical city/ Al - Najaf Al - Ashraf from 1 / 11 / 2012 to 30 / 4 / 2013.By the end of the semen liquefaction time, the 72 samples were divided into two groups : - the fertile men, (17), and the second is represented by the Infertile Normozoospermic (23) and the Asthenozoospermic (32). The age average was (1.36±30.9) , (0.98 ±29.6) and (1.63± 31.4) years respectively. The samples were restricted to the non smoking patients only. The study results show a significant (P< 0.05) decrease in the sperm concentration, the percent of the progressive forward movement sperm, the sperm viability percent and the semen volume, and a significant (P<0.05) increase in the round cell concentration, liquefaction time, Malondialdehyde concentration and the percent of the abnormal chromatin of the Normozoospermic and the Asthenozoospermic patients comparing with the fertile men. The results also show a negative correlation (P<0.05) between the (MDA) concentration and the parameters represented by the sperm concentration, percent of the progressive forward movement sperm, the normal sperm percent, the sperm viability percent sperm quality index and semen volume. While it had been noticed that there was a positive relation between the MDA concentration and the parameters represented by the sperm liquefaction time, round cell concentration and the abnormal sperm chromatin percent of all the study groups. In this research the relation between the sperm chromatin damage and the other study parameters had been studied by dividing the samples according to the degree of chromatin damage. The results show a significant (P<0.05) decrease in the sperm concentration, the percent of the progressive forward movement sperm, the normal sperm percent, the sperm viability percent, the sperm quality index and semen volume, with a significant (P<0.05) increase in the liquefaction time and round cell concentration (MDA) concentration and the percent of the abnormal chromatin of the group (> 10) comparing with the group (? 10) for the fertile men, also the results show a significant (P<0.05) decrease in the sperm concentration, the percent of the progressive forward movement sperm, the normal sperm percent, the sperm viability percent and the semen volume, with a significant (P<0.05) increase in the liquefaction time and round cell concentration for the group (> 25) comparing with the group (? 25) for the infertile Normozoospermic patients. For the Asthenozoospermic patients the study shows a significant (P<0.05) decrease in the sperm concentration, the percent of the progressive forward movement sperm, the normal sperm percent, the sperm quality index, the sperm viability percent and semen volume, and a significant (P<0.05) increase in the liquefaction time and round cell concentration for the study groups represented by the group (> 20 - 40) and (< 40) comparing with (? 20). From the above results it could be concluded that the increase of (MDA) level resulted from the active (ROS) leads to an increase in the abnormal chromatin percent of the infertile Normozoospermic patients which had a negative effect on the semen parameters which could be a reason for the infertility for those patients

علاقة بعض الهرمونات والمعايير الكيموحيوية للمصابين بحصى المرارة == Relation of Some Hormones And Biochemical Variables For Patients With Gallstones

Author name: انسام فيصل ياسر الجوراني
Supervisor name: احسان ريسان ابراهيم الركابي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: صممت الدراسة الحالية لمعرفة مستويات بعض الهرمونات والمتغيرات الكيموحيوية لممصابين حصى المرارة، اذ اجريت الدراسة على المرضى الوافدين الى مستشفى الزهراء التعليمي ومستشفى الكرامة التعليمي / قسم الجراحة في محافظة واسط للمدة منذ بداية شهر تشرين الاول (2012) حت | The study was Designed to investigate some hormonal levels and biochemical variables in patients with gallstones, where the study was conducted on patients attending Zahra Hospital and Karama Hospital / Department of Surgery in Wassit province for the period since the beginning of the month of October (2012) and until the end of the month of April (2013), The number of patients (75) were aged between 15 - 70 years and the number of males (15) and females (60) compared with healthy controls and numbered (25) aged between 15 - 70 years and the number of males (5) and females (20). Blood samples were collected in volume (10 ml) blood serum was isolated to achive required measurements, which included measuring the level of the hormone leptin, thyroxine (T4), thyroid stimulating hormone (TSH), growth hormone (GH), also the study included estimate the concentrations of biochemical parameters : total cholesterol (T.Chol), higher density lipoprotein cholesterol (H DL - Chol), factor risk (RF), liver enzymes ALT and AST, total serum bilirubin (TSB), glucose, creatinine, albumin, Globulin, and total serum protein (TSP), The results of the study were as follows : .Prevalence of gallstones in Wassit province, and Females injury rate will gallstone in wassit province much higher than males with rate of 5/1. Significant increase (P <0.05) in the levels of leptin hormone and GH as well as non significant increase (P >0.05) in the levels of the hormone TSH, while there was a significant decrease (P <0.05) in the level of the hormone T4 in people who suffer from a gallstones disease compared with healthy people. Significantly increase (P <0.05) in the concentration of T.Chol, and RF, while there a non - significant decrease (P> 0.05) in the concentration of HDL - Chol. significant increase (P <0.05) in the concentration of ALT, AST, TSB, and glucose with a non - significant increase (P>0.05) in the concentration of createnine. significant decrease (P <0.05) in the concentration of albumin, TSP, while an increase is not significant (P>0.05) in the concentration of globulin. There is effect of sex, age, BMI, and duration of the disease for patients with gallstones compared to healthy persons. There is effect of the taking contraceptive pills for female patients infected with gallstones compared to patients female non Taking contraceptive pills.

دراسة مناعية نسجية للاستجابة المناعية لسرطان الغدة اللبنية المغروسة في الفئران البيض == Immunological And Histological Study For Immune Response To Implanted Murine Mammary Adenocarcinoma In Albino Mice

Author name: نور ابراهيم عبد الزهرة شبر
Supervisor name: احمد حميد عبود العزام | جميل جري يوسف الحميداوي
General topic: Biology
Specific topic: Microbiology - Immunology
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: يعد السرطان واحدا من المخاطر الاساسية التي تهدد حياة الانسان في مختلف بلدان العالم، لان هذا المرض لا يقف عند عضو معين فهو ينتشر الى كثير من اعضاء الجسم الاخرى ليفتك بها، ومن هذا انطلقت الدراسة الحالية التي اجـريت للفترة من تشرين الاول / 2012 م ولغاية اب | The Cancer is one of highly risk factors that threats the human life world wide, because the disease has metastatic property from primary to secondary sites. This study was carried out during the period extending between October 2012 and August 2013, in laboratories of Faculty of Veterinary Medicine & Faculty of Education for girls in University of Kufa. It is aimed to follow up the changes in volume of growing tumor in female albino mice which were injected with cells of murine mammary adenocarcinoma.The histological changes, immunohistochemical examination investigate the distribution of CD8+ T lymphocytes were also studied.The changes of IgG and IgM titers in serum were also investigated. The study included use of 40 female albino mice at age 6 - 8 weeks, 25g weight average.All mice were injected by cells of murine mammary adenocarcinoman (AM3) in subcutaneous of femoro - dorsal region, and ascending to cervical region. The tumor was successfully implanted into 28 mice.These mice divided into four groups, each contains seven tumor - bearing mice.The animals of the first group subjected to the investigations after 48 hrs from implantation , while those of 2nd, 3rd & 4th groups after 10, 20, 30 days respectively. Seven healthy mice were considered as control group to comparison of immunological tests. Tumor volume & relative tumor volume were measured in different experimental groups. Histological section were prepared by routine histological techniques that including the steps of dehydration, clearing, infiltration, embedding, cutting & staining by hematoxylin and eosin stains, then microscopical examination had been done by optic microscop. The results indicated there was significant increase in tumor volume and relative volume with advancing of tumor age in animals of 2nd, 3rd & 4th groups where compared with first group. The gross examination of the animals of different groups showed that the tumor grew as irregular projection with loss of hair and erosion of overlying skin with milky discharge. The post - mortem examination revealed that the tumor mass was divided into small lobules with various coloures and the tumor mass surrounded by fibrous tissue which increase in thickness as the tumor increased in the age. Extensive areas of necrosis were noticed in the centers of the tumor masses. The microscopic examination of tumor masses in the first group raveled there were sub cataneous small aggregation of tumor cells at the implantation region as well as dispersion of the implantated tumor cells. The inflammatory cells, especially neutrophils were infiltrated. In the second group, the tumor masses showed lobulation by fibrous septa with proliferation of new blood vessels with congestion of them. Infiltaration of inflammatory cells, particularly the lymphocytes. The tumor cells arranged as ribbons and acini, characterized by hyperchromacia pleomorphism, high N/C ratio as well as appearance of mitotic figures. The histological features of the third group revealed increase of amount of fibrous tissue extensive necrotic areas and loss of architecture.These appeared in the fourth group in addition to the presence of cellular debris with dead inflammatory cells. The immunohistochemical study showed no CD8+ T cells were infiltrated in the mass of first group but the little infiltration of these cells was show in the second group. The highest infiltration of the CD8+ T cells was appeared in the third group. while the little cells were discharged in the fourth group. The results of the single radial immunodiffussion assay showed that the implantation with mammary adenocarcinoma had significant effect in increasing the titer of IgM after (48) hours and (30) days from implantation of tumor compared to control group, while the titer is not significant after (10) and (20) days from implantation of the tumor compared to control group. Also the results revealed a significant decrease in the titer of IgG after (48) hours and (30) days from implantation of the tumor, while were no significant increase in the titer of immunoglobulin after (10) and (20) days from implantation of the tumor, compared to the control group. From all the above, We concluded that there was variable histological changes as the age of tumor advanced these changes were characterized by increase of amount of tumor cells in the first groups and occurrence of necrosis in late groups, as well as increase the fibrous tissue as the tumor age advanced. The infiltrative CD+8 lymphocyt was also variable as change of tumor age. The implantation of tumor in mice groups had marked effect in stimulation of humoral immune response through increase of IgM & IgG titers.

التعبير الجيني للمورث Erg11 وعلاقته بالمقاومة وظاهرة رجوع النمو لبعض عزلات خميرة المبيضات البيضاء المختبرة تجاه مضادات الازول الفطرية == Gene Expression of Erg11 And It’s Relation With Resistance And Trailing Growth Phenomena of Some Isolates of Candida Albicans Tested Against Azole Antifungals

Author name: حيدر عبد الحسين عباس
Supervisor name: عدنان حمد عبيد الحمداني
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: هدفت الدراسة الحالية الى الكشف عن دور المورث Erg11 والتعبير الجيني النسبي له في مقاومة خميرة المبيضات البيضاء C.albicans وعلاقته بنشوء عزلات مقاومة او ظاهرة رجوع النمو Trailing growth لهذه الخميرة عند اختبار حساسيتها الدوائية تجاه بعض مضادات الازول (الفلو | The current study aimed to detect the role of Erg11 gene and its relative expression in the resistance of Candida albicans, and its relationship with the emergence of resistace or Trailing growth isolates when tested its susceptibility against azoles agenst (fluconazole, ketoconazole). A total of 120 specimens were collected from patients of both gender with different ages whom infected with oral thrush, uro - genital and Gastrointestinal candidiasis and admitted the General Teaching Hospital and materinaty and children Teaching Hospital in AL - Diwaniyah city during the period from December/2012 to February / 2013. The isolation and identification of yeast isolates were done based on their morphological, Cultural and biochemical tests in addition to use the confirmatory systems such as the growth on Chrom Agar candida, Api Candida and Tobacco Agar medium to distinguish C.albicans from other yeast spp. Also, some virulence factors of C.albicans such germ tube formation, chlamydospores production, growth at of 45 c and resistance to cycloheximide were detected. The antibiotics sensitivity test was used in two method (disk diffusion & dilution) to determine the sensitive, trailing growth, resistance isolates and minimal inhibitory concentration (MICs) toward azole agents. The qRT - PCR was used to detect the relative quantitative of Erg11 gene expression and its relationship with resistance to azole and emergence of trailing growth phenomena in the presence of Fluconazoles and ketoconazole. The isolation and identification results revealed that the isolation percent of C.albicans was 47.05 % incomparison with other Candida species that were 15.3%, 11.76 %, 8.23 % and 5.9 % for C.krusi, C.dubliniensis, C.tropicalis, C. parapsilasis and C.glabrata , respectively. The antibiotic susceptibity tests of C.albicans showed the presence of resistance precent (38.89%) to fluconazole and the most of sensitive isolates revealed a trailing growth phenomena in the zone of growth inhibition of floconazole in a ratio 55.56%. While the resistance percent to ketoconazole was 27.78 % and the sensitive isolates showed the trailing growth in a ratio (38.89%). The statistical analysis showed a significant differences (P <0.05) among tested treatments. The MICs values of fluconazole against C.albicans isolates were 8, 16, and 64 Mg/ml for sensitive isolates and were 0.125 and 0.5 Mg /ml for resistance isolates and the trailing growth phenomena occurred at MICs value ? 8 Mg/ml within 24 hours of incubation while the values became ? 64 Mg/ml after 48 hours of incubation.There was a significant differences (p< 0.05) among tested treatments isolates incubated at 24 hours while there was no significant differences (P>0.05) at 48 hours of incubation. In respect with Ketoconazole, the resistance of C.albicans was increased after 48 hours of incubation at MICs values 0.125, 0.25, 0.5 and 1 Mg /ml while its was sensitive at values 4, 8 and 16 Mg /ml and the trailing growth phenomena occurred when the MICs ? 2 Mg/ml after 24 hrs of incubation, while it reached to ? 8 Mg/ml after 48 hrs of incubation. There was a significant differences (p< 0.05) among tested treatments incubated after 24 hours while there was no significant differences (P >0.05) among treatments after incubation 48 hours. The results of relative quantitative gene expression for both genes revealed a significant differences (p< 0.05) in gene expression levels through the occurrence of increased in the transcription of mRNA quantity among tested treatments.The up - regulation of Erg11 and fold change in mRNA transcription was 2.498 fold compared with 1.0 for control treatment after 24 hrs of incubation in the presence of antifungal, while the increasment was 12.606 fold after 48 hrs of incubation of isolates with antifungals.

دراسة بعض الصفات الفيزيائية والكيميائية والحياتية لمياه نهر دجلة ضمن مدينة تكريت == Study of Some Physical, Chemical And Biological Properties On Tigris River In Tikrit City

Author name: بشار طارق اسماعيل هلال الشنداح
Supervisor name: رياض عباس عبد الجبار
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تهدف الدراسة الحالية للتعرف على بعض الجوانب الفيزيائية والكيميائية والحياتية لمياه نهر دجلة ضمن مدينة تكريت ولمياه المخلفات السكنية في محطة المعالجة.جمعت العينات لمدة سبعة اشهر اعتبارا من شهر تشرين الثاني 2006 وحتى نهاية شهر ايار 2007 وبمعدل مرتين شهريا | The present study aimed to find out some physical, chemical and biological aspects of Tigris river passed in Tikrit - city, and population residence in the treatment unit.Samples were collected over the period of seven months during November 2006 to the end of May 2007 biweekly. Four stations were selected for sample collections over the distance of 25 km. Two stations were in Tigris river and two were in the treatment unit. The first selected station was close to the old water purification unit in Tikrit - city. The second station was selected at the treatment unit in AL - Dubaee before the mechanical treatment unit. The third station was selected at the treatment unit after the biological treatment. The fourth station was selected in Tigris river about ten km south of the treatment unit at AL - Dijla town. The study involved an investigation of some physical, chemical and biological aspects of Tigris river and the residence waste discharges to the river. This included air and water temperature, conductivity, PH, dissolved oxygen, BOD, Turbidity, total hardness, calcium and magnesium hardness, chloride, total alkalinity, nutrients (i.e., phosphate, nitrite, silica) , and the concentrations of heavy metals (i.e., cadmium and cupper). The biological factor included the total bacteria counts cultured on nutrient agar. Results of the study clarify the close relation between air and water temperature which varied (8.5 - 36) C0 and (9 - 27) C0 respectively. Conductivity values were relatively high and ranged (128 - 3530) microsemens/cm. River water almost was alkaline during the period of the study. PH values of the first and fourth stations were close in the river, although a value of 6.12 was recovered at station on during May. In all stations, PH values ranged (8.8 - 6.12). River water has a good ventilation, dissolved oxygen values ranges (8 - 11) mg/L at the first and the fourth stations, while these values were (0.1 - 1.34) mg/L at the second and third stations. Mean dissolved oxygen values pointed out the availability of good oxygen concentration for the continuity of living organisms which ranged (0.3 - 5.8) mg/L in Tigris river, while BOD values at the stations second and third were high and ranged (5.6 - 131.2) mg/L. Turbidity was high during April at the stations one and four and ranged (2.03 - 788) NTU in all stations. Total hardness in Tigris river was slightly low - very hardness and ranged (120 - 360) mg/L at the first and fourth stations, while these values were (180 - 480) mg/L at stations two and three.The high total hardness values coincided with the high conductivity values. Concentrations of calcium ions responsible on the calcium hardness ranged (211.4 - 120.7) mg/ L was higher than magnesium ions responsible for magnesium hardness range (5 - 265) mg/L.Records of chloride ions ranged (14.16 - 169.99) mg/L at all stations. However, chloride values at stations first and fourth were among the allowed world values and ranged (14.16 - 42.48) mg/L.Alkalinity values were due to the carbonate and bicarbonate and ranged (35 - 725) mg/L.phosphate values ranged (0.168 - 25.2) µg phosphor atom - phosphate/L. Low nutrient values recorded in Tigris river, while the highest values recorded at second station (treatment unit).Nitrite values fluctuated at all stations and ranged (0.078 - 14.778) µg Nitrogen atom - Nitrite /L. Silica values ranged (648 - 5961.6) µg silicon atom - silica /L. Heavy metals values (Cadmium and Cupper) clarify that, Cadmium were (un sensible), except one value (0.008) mg/L that recorded at November, and un sensible for Cupper.Results of the statistical analyses of all involved factors showed significant differences at stations first and second, and between the first and third stations as well as the fourth station. While there was no significant differences between the second and fourth stations. And no significant differences between stations first and fourth.Total bacterial counts indicated high bacterial counts, and the average of total bacterial count ranged (2.605X105, 5.44X108, 2.265X108, 2.385X105) / ml at the four stations respectively, so the Tigris river considered pollutant - very pollutant.

دراسة شكليائية ونسجية للقناة الهضمية في سمكة الخشني Liza abu Heckel.1843 == Morphological And Histological Study of The Digestive Tract In Liza Abu (Heckel, 1843)

Author name: حنان رعد ضياء حسين
Supervisor name: وجدان بشير عبد
General topic: Biology
Specific topic: Zoology - Histology
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: The morphological and histological structure of digestive tract in one species of Iraqi fish that resides in Iraqi inland fresh water (Liza abu) was studied. In this study used (43) fish were in different lengths, the gut of these fish have removed to study the relationship between the length of gastrointestinal tract and the standard body length as well as for morphological and histological study. The value of relative digestive tract length (RGL) was 2.9. Intestine was the longest part of the digestive tract, the average length of intestine was (32 cm). The shape of the folds of the mucous membrane lining the walls of digestive tract showed some differences in different parts of digestive tract, in esophagus the folds were longitudinal running along the wall and it was straight, unbranched and the mean of their number was (9 - 8) folds. In the stomach the folds were longitudinal and (7 - 6) in their number, they were branched into fingerlings structures. In pyloric caeca, anterior and posterior part of intestine and rectum the folds were fingerlings in shape, unbranched and their number were (20 - 19), (27 - 26), (25 - 23), (19 - 18) respectively. Histological study was shown that the wall of the digestive tract is consist of four major coats, from the inside to the outside they were as follows : Tunica Mucosa which characterized by its clear three parts, epithelial tissue, lamina propria and muscularis mucosa. Epithelial tissue in esophagus was thick stratified squamous epithelium, in stomach was thin simple columnar epithelium and gradually increase in length in each of pyloric caeca, intestine and rectum and it was simple columnar epithelial tissue. Mucous which is secreted in esophagus is made of a large proportion of neutral and acidic mucopolysaccharide, while the mucous secreted in the cardiac stomach consist of a large proportion of neutral mucopolysaccharide and less proportion of acidic mucopolysaccharide, either, in the pyloric stomach is consist of very small proportion of both neutral and acidic mucopolysaccharide, in pyloric caeca mucous consist of a small proportion of neutral mucopolysaccharide and less proportion of acidic mucopolysaccharide, and the mucous secreted in an anterior, posterior parts of intestine and rectum consist of small proportion of acidic mucopolysaccharide and large proportion of neutral mucopolysaccharide. Lamina propria consists of connective tissue containing capillaries and lymphoid cells and it represent the basis of the folds, in the cardiac stomach it contains simple tubular glands. Muscularis mucosa characterized by the presence of muscle fibers that are not continuous, the higher thickness of this layer was in pyloric stomach. Tunica Submucosa consists of connective tissue that contains capillaries, the higher thickness of this coat was in the stomach and less thickness of this coat was in the rectum. Tunica Muscularis consists of two layers of smooth muscle fibers, the internal layer has a circular arrangement, the external layer has a longitudinal arrangement in stomach, pyloric caeca, intestine and rectum. either in esophagus tunica muscularis was arranged in inverse manner. The circular and longitudinal muscle fibers in esophagus were skeletal muscle fibers, and they were smooth and non - striated in stomach, pyloric caeca, intestine and rectum. the higher thickness of this coat was in the stomach and less thickness was in the rectum Tunica Serosa is present in all regions of digestive tract.

التمنيع والتحدي لبكتريا Citrobacter freundii فاقدة الجدار المسببة لخمج السبيل البولي == Immunization And Challenge For Walled Citrobacter Freundii And Walled Defective That Causing Urinary Tract Infection

Author name: عبد الرسول عويد عداي الشبيب
Supervisor name: قاسم نجم ثويني
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: Cryptic bacterial infection has been studied in urinary system to Human, where 50 underwent urine samples from patient mostly based consulting auditors Hilla public Education Hospital for the period from November 2012 until Feb. 2013. Patients ranged in age from 20 - 50 years males and females bacterial infection rate was 70% distributed between the cell wall defective bacteria had 38% and 32% cell - wall bacteria and the results showed 30% as negative cases. Amicroscopic examination of the urine sludge Showed clear sovereignty of non - specialized immune response type of mixed cell 34% followed by mononuclear cell 30%. Samples were cultured on routine media (Blood agar , Macchonky agar) and Modified media (Variant agar, Variant broth). Out put diagnosed by bacterial system diagnostics, phen - otypic, microscopically and biochemistry by using the special test for microorganism diagnosis and using API 20 E and the use VITEK 2 to confirm some samples results. Characterized by colonies of cell wall defective bacteria grow on variant agar it resembles fried egg which could seen after staining Deinse stain. Citrobacter freundii recorded 12%, the highest rate among cellwall defective isolates. Sensitivity test for bacterial isolates were screened from U.T.I both two type (CWB & CWDB) showed the highest rate of sensitive to Impinem 100% represented CWDB, as well as the highest rate of resistance to Ampicillin 100% represented by the same group of isolates. experimental study with urinary tract infection by injecting bacterial stuck of Citrobacter freundii to the three groups of rabbits : first group ( 3 Rabbits ) : immunized with viable surface antigen extracted from CWDB, second group : ( 3 Rabbits ) immunized with heat killed surface antigen extracted from CWB, the third group (4 Rabbits) represented control group it been treated with sterile normal saline. the injection process through urethra, and then underwent a histological study, was histological damage to the members of the animal - derived non - immunized (control group) seemed clear was the appearance of symptoms of the total cellular infiltration and disruption of the mucous layer and epithelial and other symptoms. The immunized animals have shown their ability to defy injury. It is the study of serological reactions to bacterial antigens Citrobacter freundii immunized with vaccines for rabbits given high caliber agglutination with its specialized vaccines and low with adversity and that the absorption and the corresponding and variant antigen does not remove the agglutination effectiveness with immune sera prepared suggesting that the absorption is incomplete and that the difference between the wall and a wall defective in terms of antigenic is quantitative and qualitative.

دراسة بعض متغيرات الخطوط الجلدية للبنان ومجاميع الدم لذكور اطفال التوحد

Author name: اسراء جعفر صادق السعدي
Supervisor name: نصر فرحان عبد الله
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: Arabic
University location: Baghdad
First pages:

دراسة وبائية وسريرية لمرض الجيارديا Giardiasis في اطفال مدينة الشرقاط وضواحيها == Parasitic Study of Giardiasise Among Children In Sharqat City

Author name: علي حمد طلال موسى الجبوري
Supervisor name: حسين ساهر اسود العبيدي | عبد الله حسين عبد الله الجبوري
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت هذه الدراسة لبيان تاثير طفيلي الجيارديا المعوي على صحة الاطفال في قضاء الشرقاط لـ(250) طفل، تم اختيارهم بصورة عشوائية، اذ تراوحت اعمارهم من شهر - 15 سنة وللمدة ما بين شهر تشرين الاول 2006 لغاية شهر تشرين الاول 2007، تم فحص عينات الغائط من كل طفل بال

تاثير تدخين السكائر على فعالية انزيمات AST، ALT وALP ومستويات الشوارد في مصل دم المدخنين == Effect of Cigarette Smoking On The Activity of Serum AST, ALT, ALP And Levels of Electrolytes In Blood Serum of Smokers

Author name: حسام هاشم محمد العزاوي
Supervisor name: حميد محمود مجيد
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: للتدخين اثر مباشر وغير مباشر على معظم اعضاء الجسم، وربما يحدث تاثيره في اقل من ثانية عند استنشاقه ووصوله الى الحويصلات الهوائية وانتشاره في الاوردة الرئوية. ولدراسة التغير الحاصل في : مستويات انزيمات الكبد (AST) Aspartate aminotransferase، Alanine aminotr | Smoking effects directly and indirectly on most organs of the body, this effect may occur in less than a second when inhaled and reach the alveoli and prevalence in the pulmonary veins, to study the change in the : levels of liver enzymes (AST) aspartate aminotransferase, alanine aminotransferase (ALT) and (ALP) alkaline phosphatase and electrolytes (Na +, k +, Cl - , Ca2+, Fe2+ and PO43 - ) in the blood serum of male, Collection of 100 blood samples of male smokers which divided into three groups depending on the smoking period : 1 - first group of 5 - 10 years Comprises 35 smokers, the age range from 25 - 40 years. 2 - second group of 10 - 20 years Comprises 34 smokers, the age range from 25 - 40 years. 3 - third group of more than 20 years Comprises 31 Smokers ages ranged from 25 - 40 years. The above groups Compared with 40 person Non - smoker (group 4), withage range from (25 - 40) year, for the period from November.1st. 2013 to April.1st. 2014, Within the district of Baquba / Province of Diyala.. Results showed : 1 - Increasing in activity of serum liver enzymes AST and ALT (p ?0.01) for a period 5 - 10 years of smoking, and at level of (p?0.001) for both of the periods of smoking 10 - 20 years and more than 20 years as compared to control. While the levels of ALP increased significantly (p?0.01) for three groups as compared with control. 2 - There is no significant differences in the levels of electrolytes Na+, k+, Cl - in blood serum. 3 - There is a significant decrease in the levels of Ca2+ (p?0.001) for the three groups compared to control, while the iron concentration levels increased significantly (p?0.001) in blood serum of the three groups of smokers compared with control. phosphorus levels showed increasing significantly (p?0.05) in both groups of smokers 10 - 20 years and for more than 20 years compared to control. Conclusion from the above : presence of clear effects of smoking on the liver enzymes activity, calcium, iron, phosphorus, while there is no clear effects on electrolytes Na +, k + and Cl - .

دراسة بيئية لبعض انواع صنف بطنية القدم الارضية في ثلاث محافظات وسط العراق == An Ecological Study For Some Terrestrial Gastropoda Species In Three Provinces Middle Iraq

Author name: ايمان حسين عبد
Supervisor name: عماد الدين عبد الهادي المختار
General topic: Biology
Specific topic: Environment
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: اجريت الدراسة الحالية للتعرف على انواع صنف بطنية القدم الارضية المتواجدة في بعض محافظات وسط العراق (بغداد, بابل, كربلاء). اذ تم تسجيل الملاحظات الحياتية والتعرف على بيئاتها المفضلة وقياس العوامل البيئية ذات الاهمية. حيث تم تشخيص ثلاثة انواع وهي : Cornu as | The present study was conducted to identify the terresterial gastropoda species located in some provinces of middle Iraq (Baghdad, Babylon and Karbala). They were record Biological observations on their environment and learn about their prefer environments and to measure the important ecological factors. Three species were diagnosed : Cornu aspersum specie belong to Helicidae family, Monacha cantiana and Candidula gigaxii species belong to Hygromiidae family. Monthly sampling was conducted from (October 2013 - July 2014) of the three provincs on two sites per each provinc, Where the samples were collected randomly by five repeaters of one square meter once a month. The study also included soil ecological properties measuring : soil temperature, soil pH, Electrical conductivity, percentage moisture, organic matter content and soil texture.The results showed soil temperature values ranged between (8 - 30) C?, soil pH (7.33 - 7.96), Electrical conductivity (0.7 - 4.1) ds/m, percentage moisture (9 - 33) % , organic matter content (0.81 - 2.88) %. The results showed percentages rate for clay, silt and sand for Jadiriyah (38.39, 49.18, 12.43)%, Zafaraniya (36.66, 51.53, 11.81)%, Alexandria (37.93, 52.54, 9.53)%, Hindyia dam (40.54, 53.05, 6.41)%, Hosseinie (36.28, 45.81, 17.91)% and Twerij (37.79, 50.20, 12.01)%. Generally soil texture clay silt sandy soil for all study sites. The relative abundance of the studied species was recorded, The lowest value for Candidula gigaxii specie was 7.3 % in March in Hindyia dam, while the highest value was recorded for Cornu aspersum specie 75.0 % in December 2013 in Jadiriyah. Rates of population density (individuals / m 2) were calculated for the studied specimens and the results showed the presence of significant differences when comparing the study sites during research period. The population density rates for species the results showed the lowest value for Candidula gigaxii specie 0.4 (individual/m²) in October 2013 in Jadiriyah, and the highest value for Cornu aspersum specie 19.4 (individual/m²) in Janeuary 2014 in Jadiriyah. The results showed that Candidula gigaxii specie is found in sites (Jadiriyah, Zafaraniya, Hindyia dam(along research period, While the results did not record the presence of it in sites (Alexandria, Hosseinie, Twerij).The study showed presence of positive and negative significant relationships between species and soil ecological properties, as it were for Cornu aspersum specie negative significant relationship with air and soil temperature (0.827, 0.763) respectively, positive significant relationship with moisture and organic matter content (0.703, 0.880) respectively, while the results did not record significant differences with pH and Electrical conductivity (0.096, 0.168) respectively. Monacha cantiana specie has recorded negative significant relationship with air and soil temperature (0.431, 0.553) respectively, positive significant relationship with percentage moisture and organic matter content (0.368, 0.550) respectively, while did not record significant differences for pH and Electrical conductivity (0.214, 0.096) respectively. The results showed for Candidula gigaxii specie negative significant relationship of air and soil temperature (0.504, 0.322) respectively, positive significant relationship with moisture and organic matter content (0.380, 0.569) respectively, While did not record significant differences with pH and Electrical conductivity (0.088, 0.170) respectively.

دراسة بيئية للهائمات النباتية في مبزل CD4A (مبزل هور شعيب) الديوانية - العراق == Ecological Study of The Phytoplankton In Cd4A (Hor Shuaib) Drainage In Al - Diwaniya Iraq

Author name: ابتسام كاظم خضر الصالحي
Supervisor name: فؤاد منحر علكم
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: تضمنت الدراسة الحالية بيئة الهائمات النباتية في مبزل CD4A (مبزل هور شعيب) في الديوانية - العراق. للمدة من ايلول 2011 حتى نهاية اب 2012 حيث تم اختيار ثلاثة مواقع على المبزل، يقع الموقع الاول في بداية مجرى المبزل عند قرية شويخة ويقع الثاني على بعد 10 كم من | The present study deal with phytoplankton in CD4A drainage for a year starting from September 2011 to August 2012.Three sites have been chosen, the first is near shoekha village at the beginning of the drainage, Second is after the first site ten kilometers, while the third site is at the end of the drainage after Neffr sub district. The study includes measuring physical, chemical and biological properties of drainage water which includes studying quality and quantity of phytoplankton. The study shows that the temperature of the air is ranged from 12.5 - 42.1 c? and water temperature is about(11.5 - 31.5) c? light penetration from 16 - 57cm while turbidity is(49 - 2.1)NTU The study shows that the pH is limited in along the period of the study (6.4 - 8.4), Electrical conductivity is approximately about (2247 - 8030 µs/cm), the water drainage Oligohaline The dissolved oxygen values was from 3 - 8.6 mg/l and carbon dioxide values were ranged from 80.2 - 227.8 mg/l, it also show that drain water is alkaline and bicarbonate ions prevailed, total alkaline is (281.3 - 102.6)mg/l of calcium carbonate. It is discovered that the water in the area under investigation is too hard, hardness values were ranged from 1439.5 - 517.6 mg/l, while Calcium concentrations were ranged from 232.5 - 98.1mg/l andMagnesium concentrations were ranged from 220.3 - 64.2mg/l. Chloride concentrations were ranged from802.01 - 334.1µg/l, while Sulfate concentrations were ranged from 1184.9 - 421.03µg/l Plant nutrients Nitrite concentrations are about (6.22 - 0.58) µg/l, Nitrate concentrations (122.3 - 19.5) µg/l and Phosphate concentrations are about (2.09 - 0.1) µg/l, Phytoplankton types which are diagnosed during study period (143). Bacillariophyceae is dominate on other class and Chlorophyceae Cyanophyceae, Euglenophyceae, the study shows there are99, 120 and 125 species in the first, second and third site, the species that have highs number (Nitzschia, Navicula, Cynbella, Oscillatoria, chlorella, cocconeis, Amphora.Cyclotella), During the period study there are seasonal and site changes is total number of of phytoplankton which ranged about)5218.9 - 267.9 (x 103 cell/cm2. Statistical analysis showed there morale differences between locations and seasons in relation to physical, chemical and biological features also there is an interference between months, there are negative and positive, morale and in morale correlation between phytoplankton and physical, There are negative and positive, morale correlations between phytoplankton and physical, chemical features that measured during study period at probability level of (0.05).

دراسة بيئية ونوعية لمشروع تصفية ماء الكرخ في الطارمية == An Ecological And Qualitativ Study For Al - Karkh Drinking Water Plane At Al - Tarmia

Author name: احمد توفيق احمد المشهداني
Supervisor name: رياض عباس عبد الجبار
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: نظرا لما لمياه الشرب من اهمية كبيرة في حياة الانسان وضرورة ان تكون هذه المياه ضمن مواصفات ومعايير خاصة, فضلا عن ظهور العديد من مشاكل تلوث مياه الشرب في مختلف مدن العراق. دعت الحاجة لاجراء هذه الدراسة لبيان مدى صلاحية مياه الشرب المنتجة من مشروع تصفية ماء | Owing to the drinking water of great importance in human life and it is important to be under the specifications and standards, as well as the emergence of many of the problems of contamination of drinking water in various cities of Iraq. Needed to conduct this study to demonstrate the viability of drinking water produced from the project filter water Karkh (which is the largest project the liquidation of water in the Middle East, it is feeds many of the cities of Baghdad, on the side of Karkh) and compare the specifications of Iraq and the global standard for drinking water in order to investigate the potential weaknesses in the project and study the possibility of the development of such large projects as a first step to improving the situation of drinking water in Iraq. So were the tests, physical, chemical and biological common to drinking water in the project and divided into four main stations, namely, (station socket raw water, sedimentation basins secondary sand filters, pumps, distribution of drinking water), including nine stations secondary starting of the month of October - 2010 to July - 2011.The results showed high values of turbidity, and electrical condactivety and pH, and dissolved oxygen, and biological dimond of oxygen, and alkalinity, and total hardness, calcium hardness, magnesium hardness, chloride, phosphate, nitrite, silica, fluoride, sodium, potassium, and heavy metals, namely, (iron, copper and cadmium, nickel, lead, chromium and zinc), and the total numbers of bacteria, and total coliform bacteria, the fecal coliform bacteria and E. Coli in drinking water for most of the stations in the Alsta spring and low concentrations in summer and autumn.Values for water temperatures between (4 - 37.5) c, as recorded differences significant between the months of winter and spring, summer and autumn, and the values of brownish discharge ranged between (250 - 0.3) NTU as it showed the differences of the moral high values of turbidity in the winter and fall in the summer and fall in each stage of the treatmant with it, and the values of electrical conductivity was between (520 - 895) Micro Siemens / cm 3. The water Alnehrualcherb in the project was registered as a weak base pH values between (9.7 to 1.7), and it was well ventilated, as the oxygen values were high in the winter months registered clear monthly variation ranging between (5.14 - 2.3) mg / L, either values biologicaldimond of oxygen were values between (4.4 - ND) mg / L and found that basal total stations ranged from every stage of the liquidation potential in increasing concentrations of dissolved oxygen, between (160 - 100) mg / L, and found that drinking water hardness too, as that the values of total hardness ranged between ( - 215 335) mg / L as suited their values directly proportional to both the calcium and magnesium hardness ranging between (197 - 139) and (31.472 - 16.128) mg / L, respectively, while for the values of the chloride ranged between (69 - 25) mg / l, while the fluoride has recorded values ranged between (15 - 0.05) mg / L, with values ranging between sulfate (169.2 - 90) mg / L, free chlorine residual values ranged between (4.18 - 1.1) mg / L, and materials for suspended solids ranged values between (84 - ND) mg / L proven contribution stages of liquidation, all in the rates of removal of different, either TDS fluctuated values between (545 - 315) mg / L, with log Aluminum values ranged between (0.98 - 0.01) mg / L, but for the potassium values ranged between (3.2 - 2.15) mg / L, and sodium values ranging between (6.31 to 3.21) mg / liter.As for the concentrations of heavy metals did not detect any significant concentration of heavy metals measured except for iron ranging between concentrations (9.2 to 8.1) mg / L, so after the industrial plants that contain the droppings of these metals. The nutrient values were recorded between nitrite (0.024 - 0.001) mg / Trosgelt nitrate values between (1.7 - 0.16) mg / l, recorded between ammonia (0.54 - ND) mg / L, placed at either phosphate values ranged between (0.26 - ND) mg phosphorus atom - phosphate / L, while for the values of silica fluctuated between values (5.12 to 5.1) mg atom of silicon - silica / liter. As for the study of signs bacteriological ranged the total numbers of bacteria in air through (24) hours between (555 - 0) × 10 cells / 1 ml, and ranged in numbers for a period of (48) hours between (1186 - 0) 10 × cells / 1 ml, while for the numbers of total coliform bacteria fluctuated between (1700 - 0) cells / 100 liters, and fecal coliform bacteria, they ranged (1100 - 0) cells / 100 ml, and the E.coli bacteria, the numbers fluctuated between (1100 - 0) cells / 100 ml. For to match the drinking waterproduced from the projects liquefaction research found matching the values and concentrations of brownish, and connectivity electrical and pH, and dissolved oxygen, and the requirement is vital for oxygen, and basic college, and chloride, and ten college, and magnesium, nitrite, phosphate, and the total numbers of bacteria, and the numbers of total coliform bacteria, and fecal coliform bacteria and E Coli, and silica with the standard specifications for drinking water the Iraqi and global at all stations.While the study results show non - conformity of calcium concentrations in this study with the standard specifications for drinking water of Iraq and the world.

عزل وتشخيص البكتيريا Bacillus thuringiensis ودراسة تاثيرها في بعض المقاييس الحياتية لذبابة ثمار القرعيات (Diptera : Tephritidae) Dacus ciliatus == Isolation And Identification of Bacillus Thuringiensis And Studying Its Effects On Some Biological Parameters For Dacus Ciliatus (Diptera : Tephritidae)

Author name: ادريس صالح جليل
Supervisor name: عدنان نعمة عبد الرضا العزاوي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: تضمنت هذه الدراسة عزل البكتيريا Bacillus thuringiensis من مصادر بيئية مختلفة في محافظة ديالى وتشخيصها بالطرق الكيموحيويه والوراثية والجزئية، اذ جمعت 51 عينة من ترب مختلفة وهي(زراعية وحديقة وغير زراعية) وكذلك من الماء، من خمسة اقضية في محافظة ديالى (بعقوبة | This study included the isolation of pathogenic bacteria Bacillus thuringiensis from different environmental sources in Diyala province, diagnosis biochemical and genetic partial ways, Mar 50 samples collected from different soils, namely, (agricultural soils and garden soils and non - agricultural soils) as well as from the water, from five districts in the province of Diyala (Baquba, Khalis, Muqdadiyah, Khanaqin, Balad Ruz) for the period from 1 \ 9 \ 2014 to 20 \ 11 \ 2014. Showed the results of biochemical diagnosis and the presence of four samples and by percent 8% It was of a negative for transplantation bacterial and 46 sample growth by percent 92% It was of a growth positive for transplantation bacterial and found that 41 samples of which by percent 82% belong to the genus Bacillus and the species of Bacillus thuringiensis and the percentage of bacteria isolated of agricultural soils 94% and soils garden 93% and water 75%, and soils non - agricultural 44%. The pathogenic bacteria Bacillus thuringiensis of positive bacteria a gram dye and spore forming and The most of the natural habitat this bacterium is soil and it is capable of producing the diversified varieties of crystalline proteins with insecticide property. All isolates were tested for this bacteria that which were able growth on the media selective Acetate Sodium Selective and the production of insecticidal crystalline proteins (ICP) When staining by dye Coomassie brilliant blue (CBB) to conduct genetic partial diagnosis using several extraction (Mini gDNA Bacteria kit) processed by Bioneer company was the purity of DNA extracted ranging between (20.1 - 1.22) for all selected isolates, have been diagnosed with the bacteria at the level of genus and species Using the gene 16S rDNA with a molecular weight of 380 base pairs where the results showed polymerase chain reaction (PCR) that all isolates contain this gene and the same molecular weight which is 380 base pairs, also was diagnosed isolates containing the gene component crystalline proteins which cry a e molecular weight 200 base pairs using primer a specialist for this gene in polymerase chain reaction showed the results of gene doubled that all isolates contained the gene and the same molecular weight of 200 base pairs. After making sure of pathogenic bacteria as belonging to the genus Bacillus and species Bacillus thuringiensis according to their chemical diagnostics biochemical and diagnostic genetic partial, attended different concentrations They (1, 2, 3, 4, 5) g/L of thebio preparation for these bacteria to study their impact on the different roles of Cucurbits fruit fly Daus ciliatus. Has been tested the effect of different concentrations of bio - preparation of these bacteria isolated from soil and water on the roles of this insect which eggs and larvae and pupa and AdultsThe results of test the effect of bio - preparation of these bacteria on the eggs of age (1 - 2) Since the highest rate of hatchling (3.2) and by (89%) at a concentration of 1 g / l of bio - preparation and lowest hatchling (0.6) and by (19%) at a concentration of 5 g / L of bio - preparation comparison with control (3.2) and by (96%) for the isolated bacteria from soil Since the highest rate of hatchling (3.1) and by (92%) at a concentration 1 g / L of bio - preparation and lowest hatchling (2.1) and by (62%) at a concentration of 5, 4 g / L of bio - preparation comparison with control (3.2) and by (96%) for isolated bacteria from water, and on The last larval phase, The highest the value of the rate of highest (2.0) and by (99%) at a concentration of 5 g / L of bio - preparation and The less the value of the rate of loss (0.5) and by (26%) at 1g / L of bio - preparation concentration compared with the control treatment (0.07) and by (%3) for isolated bacteria from soil.reached its highest value the loss rate (1.2) and by (57%) at a concentration of 5 g / L of bio - preparation and less the value of the loss rate (1.0) and by (6%) at a concentration 1 g / L of bio - preparation comparison with control (0.07) and by (3%) for the bacteria isolated from water and on pupa age(6 - 7) day, reaching the highest rate of emergence of pupa (3.2) and by (92%) at a concentration of 1 g / L of bio - preparation and less the emergence of pupa rate (0.4) and (13%) at a concentration of 5 g /L of bio - preparation comparison with control (3.2) and by 96% for bacteria isolated from soil reaching the top of the emergence of pupa rate (3.1) and by (92%) at a concentration of 1 g / L of bio - preparation and less the emergence of pupa rate (2.1) and by (62%) at a concentration of 5 g / L of bio - preparation comparison with control (3.2) and by (96%) for bacteria isolated from water. and on kill adult, The highest killing rate for males (1.6) and by (32%) and females (1.5) and (30%) at a concentration of 5 g / l of bio - preparation and less killing of the male rate (0.5) and (10%) and females (0.4) and by (13%), comparison with control males (0.1) and by (2%) and females (0.1) and by (2%) for the isolated bacteria from soil. The highest kill the male rate (0.8) and by (32%) and females (0.7) and (30%) at a concentration of 5 g / L of bio - preparation, and less killing of the male rate (0.2) and (4%) and females (0.4) and by (13%) at a concentration of 1 g / L of bio - preparation comparison with control males (0.1) and by (2%) and females (0.1) and by (2%)for the isolated bacteria from water and one female productivity of egg reached its highest production rate eggs 84.5 eggs per female at a concentration of 1 g / L of bio - preparation, and less egg production rate of 54.5 eggs per female at a concentration of 5 g / L of bio - preparation comparison with control eggs productivity rate of 87.5 egg female for isolated bacteria from soil reaching its highest egg production rate (85%) eggs per female at a concentration of 1 g / L of bio - preparation, and less egg production rate (79.5%) eggs per female at a concentration of 5 g / L of bio - preparation, compared with treatment control productivity rate of whites (85.5%) eggs per female for isolated bacteria from the water
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