Author name:
محمد عدنان هاشم ال ابليش
Supervisor name:
طالب عويد الخزرجي | عقيل حسين العاصي
Abstract:
تضمن البحث الحالي دراسة مقارنة مظهرية وتشريحية وكيميائية ووراثية لثمانية انواع تابعة للجنس Heliotropium L. (Boraginaceae) في وسط وشمال العراق هي H.bacciferum Forssk., H.ellipticum Ledeb. H.europaeum L., H.lasiocarpium Fisch., H.noeanum Boiss., H.ramosissi | The current research study is a comparative morphological, anatomical, chemical, genetical of the genus Heliotropium in mid and north of Iraq between eight species belonging to the genus of the family Boraginaceae, and the study included some morphological characteristic of the roots, stems, leaves, flowers (calyx, corolla, stamens, pistils), inflorescence, fruits and pollen grain shows that there are clear differences between the species under study. The study included aspects of anatomy and the differences included the qualities of epidermis leaf and stem and stomata as well as study transverse sections of roots, stems, leaves and petiole and peduncle, fruits, Nutlets and seeds, also studied the system of Venation in the leaf where he studied the type of venation as well as study the direction of midrib, thickness, branching as well as the study of secondary veins, Arrangement, Aerioles and Veinlets that showed clear differences between species.The study showed that pollen grains in the species are Tricolporate in all species except H.bacciferum it contains four grooves and pollen grains of contain Psaudotricolporate found in all species except H.lasiocarpium was completely absent of false grooves have distinguished this species by this character, showed the study of pollen grain Psaudotetracolporate in species H.bacciferum only and did not appear in the rest of the species. the study noted that there are variations evident in the two axes equatorial and polar as well as in the length of the groove, width, the rate of the thickness of the wall of pollen grain, the rate of diameter germination aperture in all species. The study showed clear difference between species in dimensions of upper and lower epidermis leaf, as well as a study stomata index on the surfaces of upper and lower leaves where show that all species were the lower surface more than the upper surface in density of stomata and Trichomes were studied in all parts of plants under study it was observed that there is variation in the distribution of hair and their locations as well as the lengths of hairs and thickness of the walls and shape of apex and base. Studied species in pathway of photosynthesis showed that two species are C4 plants H.noeanum, H.suaveolens either species H.bacciferum, H.ramosissimum, H.lasiocarpium was belong of intermediate C3 - C4 species while the rest were from C3 plants after analysis qualities and similarities of these qualities were very important to isolate the species from each other because present of bundle sheath, thickness of leave in cross section, thickness of cuticle, thickness of chlorenchyma and collenchymas tissues, frequency of stomata and it distribution, the number of aerioles and veinlets density and size. Has also been studying the chemical content of alkaloids and found that there are six alkaloids isolated for the first time from inflorescences for plants belonging to the genus Heliotropium were isolated using the technique liquid chromatography for identified six alkaloids compounds (indicine and supinine, indicine - N - oxide, heleurine, heliotrine and lindelofidine), which included the study of compounds Alkaliods type, concentration and percentage and the pyrrolizidine alkaloids that have a biological importance of cure tumors and cancers. The study showed high percentage of heliotrine in four species of Heliotropium H.bacciferum, H.ramosissimum, H.europaeum, H.noeanum were (48%, 54%, 51%, 54%) while heleurine was high percentage in two species H.suaveolens, H.supinum were (33%, 56%), while the study showed lower percentage of indicine - N - oxide in three species H.ramosissimum, H.noeanum, H.supinum were (2%, 4%, 1%). The current research identified for the first time on the content of the Glycosides from inflorescences in eight species of Heliotropium from mid and north of Iraq. These species are H.bacciferum, H.ellipticum H.europaeum, H.lasiocarpium, H.noeanum, H.ramosissimum, H.suaveolens, H.supinum by using the liquid phase chromatography diagnosis of six compounds (heliotrope, isopyrrolidine, isorhamnetin, naringenin, quercetein, triterpene) results showed that plant species studied vary their content of these compounds, with absent of naringenin in the species H.noeanum while absent of triterpene in species H.ellipticum, H.europaeum, H.supinum. They also showed the studied differences in concentrations glycosides and percentage of species as shown H.europaeum, H.lasiocarpium, H.noeanum, H.ramosissimum, H.supinum, H.suaveolens lower concentrations (12 - 37 mg / ml) and percentages (1 - 9%) for quercetein compared to the rest of the species studied and the species H.europaeum, H.lasiocarpium, H.noeanum, , H.supinum, H.suaveolens were higher concentrations (99 - 442 mg / ml) and percentages (26 - 51 %) for heliotrope compared to other species. These results indicate the possibility of the adoption of the content glycosides for inflorescence criterion for the separation between species as well as use for medical purposes, especially pyrolizidine and isopyrrolidine important in the treatment of tumors and cancer. Studied the genetic variation through the study of random variation indicators RAPD for eight species of the genus Heliotropium, and using 14 primer showed variation between the species under study and were divided into two groups are : 1. Group A : Included only two species H.bacciferum, H.ramosissimum where the genetic distance between these two species (0.249) where the two species are similar in genetic traits and genetic content in the absence of many bands of the primers and appear in the other. 2. Group B : Included six species of the genus Heliotropium under study and was less genetic distance between H.ellipticum, H.europaeum where (0.105) and this is a great similarity in appearance bands between the two species in some of primers, then the following two species were also two other, where he was the closest genetic distance between the two species H.lasiocarpium, H.noeanum where (0.153) and was very close two species of H.ellipticum, H.europaeum were was (0.125). The data were analyzed and characteristics after into the program (NTSYSpc) to study morphological and anatomical, chemical, genetic and find a genetic distance inter - species on the basis of similarity So was isolated species to groups and based on a large number of morphological characteristics and anatomical, chemical, genetic possible distinguish species from each other and show the variation at the level of species belonging to the genus Heliotropium.
