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دراسة مقارنة مظهرية ونسجية للاعور وعلاقته بالبيئة الغذائية في بعض الحيوانات == Comparative study Morphological and Histological of caecum in relation to feeding ecology in some animals

Author name: عطاف طلال شاكر الكرعاوي
Supervisor name: فاضل فرهود مكي الجبوري | منار محمد حسن
General topic: Biology
Specific topic: Zoology
Degree: Master
University: University of Babylon - College Of Science For Girls - Department Of Biology
Language: Arabic
University location: Babylon
First pages:
Abstract: The present study included four axis, the aim of which is to reveal the histological and morphological changes in the caecum of birds and mammals in relation to the effect of food on it, The result, showed the following. The 1st axis includes a laboratory study on the (Starnus vulgarus) since it is omnivorous birds and has the ability to change its natural feeding very fast, The aim of the experiment is to show the effect of food on the length of the small intestine and caecum. Thus, 25 birds were dissecting. The experiment lasted 40 days. The results showed that there was moral differences in the sum of body weight, small intestine length, caecum length and thickness, in both herbivorous and carnivorous feeding groups. The average body weight of the herbivorous feeding group was (60.04 ± 11.72)gm as compared with (70.48 ± 6.37)gm for the carnivorous feeding group .There was a moral differences in the length of the small intestine and caecum in both groups of birds. The average length of herbivorous group was (27.56 ± 3.61)cm as compared with (22.90 ± 1.50)cm in the other group. There was moral differences the length of caecum of both groups. The average length in the herbivorous group was (5.91 ± 1.38)cm . compared with(1.27 ± .22)mm in the other group. The amount of vegetable food consumption was much more 73 gm/day than that of the animal food 23 gm/day. The histological studies of the caecum of the Starnus vulgarus revealed that there was amoral differences in the thickness of the lumen of the herbivorous feeding and carnivorous feeding in herbivorous was(83.00 ±3.70) while in carnivorous was(21.5 ± 2.85) .The thickness of the muscular layer in the herbivorous feeder was(2.07 ±27.60 ) compared with(5.20 ± 1.92) in the other group. The 2nd axis includes a histological and morphological studies on the caecum of two different species of birds namely Anas platyrhynchos herbivorous bird and Falcon peregrinus carnivorous bird . It was found that there was amoral differences in small intestine length, caecum length and thickness between the two groups. The rate of small intestine length in duck was(8.14 ± 1.37) cm while that of the hawk measured(25.04 ± 1.35)cm. It was noticed that the length of caecum in the duck was (11.68 ± 1.54)cm as compared with(0.40±0.08)cm in the hawk. The thickness of the caecum in the duck was (0.67 ± 0.14)cm as compared with (0.17 ± 0.04)cm in the hawk. Histologically. There was amoral differences in the lumen thickness and muscular layer. Thus, the thickness of lumen in the duck was(467.50±33.21) while that in the hawk was(141.00±6.754) . The thicken of muscular layer in the duck was (20.20±6.37) while that in the hawk was(6.40±2.07) . The third axis includes a morphological and histological study of appendix of the rabbit to reveal the effect of food on it. It was noticed that the average weight of the body was (1100.05 ± 1.86)gm and the length of the small intestine was (36.51 ± 2.17)cm and the length of the appendix was (8.32 ± 1.27)cm and the thickness of the appendix was(0.61 ± 0.19)mm.the link among the small intestine and appendix length , appendix length and thickness was studied, too. It was that observed appositive correlation among the small intestine and appendix length , among appendix length and thickness, the correlation index among the small intestine and appendix length was(0.4) while that among the appendix length with its thickness was(0.5). Histologically, it was noticed that the appendix of the rabbit made of four layers namely, mucosa, sub mucosa , muscularis and serosa. It was also found that there was aplenty of goblet cell inside the simple columnar epithelial tissue of appendix lumen. It was also noticed that there was some kind of cells called endocrine(entero endo crino cyte), and that there was a massive spread of lymphoid follicles. The fourth axis : It includes a histological comparative study between the normal and infected appendix in human. The normal appendix of the human composed of four layers namely mucosa, sub mucosa, muscular and serosa. It was noticed that there was an intensive spread of goblet cell with in the simple columnar epithelial. It was also noticed that there was a presence of crypts of liberikuhn with in mucosa layer with widely spread to lymphocytes with in mucosa and sub mucosa layer. In the condition of inflamed appendix it was found large deformation in all layers. The information start in the internal lining of the appendix which is the mucosa layer then the information spread later to the other part.

التغييرات الفسيولوجية والبايوكيميائية لنبات عين البزون باستخدام حامض الساليسليك خارج الجسم == In Vitro Physiological and Biochemical Changes of Catharanthus roseus Using Salicylic Acid Under Salinity Stresse.

Author name: هديل عامر كاظم
Supervisor name: بشير عبد الحمزة محمد العلواني
General topic: Biology
Specific topic: Plant
Degree: Doctorate
University: University of Babylon - College Of Science - Department Of Biology
Language: Arabic
University location: Babylon
First pages:
Abstract: اجريت التجارب في مختبرات كلية العلوم في جامعة بابل ,خلال الفترة 2015 - 2016 ,لتقدير كمية منتجات الايض الثانوي(المركبات القلويدية : الفنكرستين والفنبلاستين)من اوراق وكالس نبات عين البزون Catharanthus roseus. تضمنت الدراسة تعقيم الاوراق وزراعتها في وسط الـ MSمضافا اليه منظمات النمو النباتية : (1 mg.l - 1) من كل من 2,4 - dichlorophenoxi acetic acid (2,4 - D) وBenzyl adenine (BA) من اجل تحفيز الكالس. بعدها اخذت كمية مماثلة من الكالس وزرعت في وسط الـ MS مع منظمات النمو ومضافا اليه تراكيز مختلفة من ملح الـ, 6, 9, 12 and 15 dS.m - 1 NaClوتراكيز مختلفة من حامض الساليسلك50,100,150 and 200 mg.l - 1,وعمل تداخلات بين الملح والحامض. وقد ارتفعت مضادات الاكسدة الانزيمية SOD,CAT,POX ومضادات الاكسدة الغير انزيمية AsA ,proline ,GSH وكذلك الـ MDAفي الكالس تحت تاثير التراكيز 9,12 and 15 dS.m - 1 من NaCl وكذلك التراكيز 150 and 200 mg.l - 1من الـSA بالمقارنة مع عينة السيطرة وكذلك مع الكالس من التداخلات 6 dS.m - 1 NaCl +50 and 100 mg.l - 1SA ازداد محتوى الفنكرستين في تراكيز الملح 9,12 and 15 dS.m - 1وكذلك في تراكيز حامض الساليسيلك150 and 200 mg.l - 1,وقد كان اعلى تركيز للفنكرستين عند التوليفة 150 mg.l - 1SA+ 12 dS.m - 1NaCl حيث وصل الى129.092 µg/g واعلى تركيز للفنبلاستين عند التركيز 50 mg.l - 1 SA حيث وصل الى16.231µg/g.. | This experiment was conducted in faculty of Science labs, Babylon University, during 2015 - 2016 for extraction, to evaluate the quantity of secondary metabolites which are Alkaloids : (Vincristine &Vinblastine) from the leaves and callus tissue of Catharanthus roseus. This study included sterilized leaves of Catharanthus plant to culture them on MS medium supplemented with plant growth regulators : 1 mg.l - 1 of 2,4 - dichlorophenoxi acetic acid (2,4 - D) and 1 mg.l - 1 of Benzyl adenine (BA) to induced callus. Then, identically callus fresh weight sub - cultured on the same MS medium supplemented with different level of salinity using 6, 9, 12 and 15 dS.m - 1,and different concentration of salicylic acid 50,100,150 and 200 mg.l - 1 and their interactions. The results revealed that antioxidant enzymes activity like Superoxide dismutase (SOD), catalase (CAT),and peroxidase(POX),and non - enzymatic antioxidants like Glutathione(GSH), proline, Ascorbic acid(AsA),and Malandialdehyde (MDA) were high in callus under 9,12 and 15 dS.m - 1 of NaCl ,and 150 and 200 mg.l - 1 of SA, than in callus control and callus grown in MS media supplemented with 6 dS.m - 1 NaCl and 50 and 100 mg.l - 1SA and their combinations. Vincristine was increased in 9,12 and 15 dS.m - 1 of NaCl ,and 150 and 200 mg.l - 1 of SA, and the higher content was in the combination 150 mg.l - 1SA+ 12 dS.m - 1 NaCl where it was 129.092 µg/g. The higher content of Vinblastine was in the concentration 50 mg.l - 1 SA where it was 16.231µg/g

تاثير داء السكري وعقار البنتوستام على تطور الاصابة بداء اللشمانيا الاحشائية في ذكور الفئران المختبرية == The Impact of diabetes and Pentostam on development the infected Leishmania donovani parasite in male laboratory mice

Author name: افراح علي عبد الامير العزاوي
Supervisor name: فاضل عباس منشد العبادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University of Thi-Qar - College Of Science - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: This study included evaluation of the role of diabetes and Sodium Stibogluconate drug (Pentostam) against infection leishmaniasis parasite in laboratory animals experimentally infected with the parasite, Where we used in this study male white laboratory rats of the strain Mus musculus Balb /c. Aggregates were divided randomly into eight major groups of eight mice, The first group were injected with saline solution at a dosage of 0.2 ml, the group was considered as a negative control, The second group were injected with parasite (1.2 × 10 6 parasites / 0.2 ml) at a dosage of 0.2 ml, The third group was injected with the parasite and alloxan at a dosage of 0.2 ml, while the fourth group were injected only with alloxan, While the fifth group were injected with a Pentostam drug at a dosage of 20 mg/kg, as well as the sixth group were injected with leishmania parasite at a dosage of 0.2 ml and Pentostam drug at a dosage of 20 mg/kg while the seventh group were injected with the parasite and alloxan at a dosage of 0.2 ml and Pentostam drug at a dosage of 20 mg/kg while the eighth group injected with alloxan at a dosage of 0.2 ml and Sodium Stibogluconate drug at a dosage of 20 mg/kg, Where it was dissected first four groups a period of 45 days while the other groups were dissected after 75 days with recorded weights of the body before and after the experiment. Also studied the histological pathological changes and taking weights for each of the liver and spleen, blood samples were taken for the purpose of knowing the effect of which occurred in the blood parameters and the level of glucose in the blood. The results of the statistical analysis of the current study showed in the second set significantly higher (P≤0.05) total weight of the body and the weight of the liver spleen, White blood cells lymph and acidophilus, while there was a significant decrease in the total number of white blood cells, neutrophils and red blood cells there was no significant difference (P≤0.05) inthe blood glucose concentration compared with the first group. The fourth group showed a significant decrease (P≤0.05) in total body weight and red blood corpuscles with (P≤0.05) blood glucose level and neutrophil cells, the groups treated with the drug showed the sixth and seventh group height body weight and white blood cells and lymphocytes and a significant decrease (P≤0.05) red blood cells, neutrophils and acidity. There was no significant difference (P≤0.05) in the concentration of glucose in seventh group. The eighth group showed a significant decrease (P≤0.05) in the total number of white and red blood cells and significant increase (P≤0.05) in lymphocytes and acid, while there was no significant difference (P≤0.05) concentration of blood glucose. The fifth group showed the treatment of the drug loss in the radiology of hepatic cells and degeneration of clear and congestion in the central vein with the infiltration of inflammatory cells within the hepatic tissue, and showed a clear exhaustion in the white pulp and the proliferation of red pulp.we observed in the histological sections of the present study in the sixth group infected with parasite and treated with pentostam, a significant improvement of the liver tissue. The spleen showed a simple propagation of the white heart. The seventh group is experimentally infected with leishmania and the alloxan The development of diabetes and the treatment of pentostam is spread with Kupffer cells and expansion in the blood sinusoids with changes in the spleen. White and the group of nematodes around it with breeding in the red pulp as in the fifth group.

دراسة انتشار ذبابتي الرمل paptasi Phlebotomus و(Diptera : psychodidae) p.sergenti ودورهما في نقل مرض اللشمانيا في محافظة ميسان == Study the Prevalence of sand fly Phlebotomus p.sergenti (Diptera : psychodidae) paptasi and and its role in the spread of Leishmaniasis in Maysan province

Author name: عبد الحسين شلويط زغير
Supervisor name: صادق صاحب علي | بسعاد عقرب العبودي
General topic: Biology
Specific topic: Zoology
Degree: Master
University: University of Thi-Qar - College Of Science
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: during field studies have been done in order to know the spread of sand flies in Maysan province during the beginning of October /2015 until the end of September 2016 through the collecting of samples from five areas that geographically different which included the areas of AL - majer AL - kabber, AL - uzair, AL - musharah , kumait and Mugharbah in the middle of the center of province .1263 insect have been collected by two types of traps light insect traps and manual insect traps. These insects included two species : papatasi and sergenti which belong to the same genus ( phlebotomus ) the number of the first species insects was more than of the second species. The number of the first species reached 85.90% while the number of the second species was 14.04% . The inset had been classified in transmitted diseases center in BaghdadThe insects have been found in the whole areas of the studying except the species sergenti which has been not found in the area of AL - majer AL - kabber . There are two peak spread times of these insects : The first is through out October and the second is the through out may . Concerning the relationship between the spread of these insects and the occurrence of Leishmaniasis positivewhere the infestation by this disease began on September November and December and the peak spread is recorded on January on the country of the inset occurrence where it begins at spring and the beginning of autumn . The spread of these insects was in flounced by the environmental factors such as temperatures and humidity . The flagellated promastigotes of leishmania parasite were not found during the anatomy of a lot of insects.Four chemical insecticides were used to control these insects where the effect of the insecticides (snake) was the strongest for killing these insects.515 infection of Leishamaniasis has been recorded in Maysan province at proportion of 506 cutaneous infestation and 9 visceral infection respectively .The rate of skin infestation was high in age groups between (1 - 10 ) year in comparison with another age groups.Concerning the types of ulcer ,the dry ulcer were dominant and the number of dry ulcers was 873 while wet ulcers were 100 concerning the diameter of the ulcer the higher infections occurred at diameter of 1 Cm which reached 873 infection . The number of patients infected by one ulcer was the highest which reached 301 infestation.The number of dry infestation in the cities was higher than in rural areas on the contrary of wet infestation . concerning the distribution of skin infestation on the body areas the higher ratio of infestation occurred in higher and lower extremities which reached 344 at proportion of 68.57%.The infestation of skin Leishmaniasis has been concentrated in the center of province which reached 334 at proportion of 66.07%.Concerning the months of the year , the majority of skin leishmaniasis was on January which reached 137 at ratio of 27.66%most of visceral leishmaniasis occurred in the patients of blood group type O proportion of 33.33% .The infection of visceral Leishmaniasis in males was higher than of females and the highest than and the highest infection of visceral Leishmaniasis was in age groups between (1 - 3) years and also the number of visceral Leishmaniasis infestation was the highest on January . Kumait areas is characterized by the presence of the highest visceral Leishmaniasis infestation.

دراسة تجريبية وجزيئية للبكتريا المصاحبة لحالات الحروق في محافظة ميسان == Experimental and Molecular Study of the Bacteria associated with burns in Maysan Governorate

Author name: حسين علي دنانه البهادلي
Supervisor name: رحمن لعيبي جلاب | حيدر خميس شنان
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: Burn injuries are considered as health problems that may sometimes lead to rapid death. Bacteria are considered as the most important causes of microbial infection associated with burns. In present study, 75 clinical samples were collection from patients with burns for the period from October 2015 to June 2016 from the lobby burns in Sadr Teaching Hospital in the Maysan Governorate, these burn swabs revealed 56 bacterial isolates, 41 (73.21%) were Gram - negative bacterial, distributed of (35.71%) Pseudomonas aeruginosa, (28.57%) Klebsella Pneuomonia, (3.57%) Escherichia coli ,Enterobacter (5.35%). On the other hand 6(10.71%)were Gram - positive bacterial. out of 75 burned swabs, 84% revealed a single pathogens and 16% shown a mixed bacteria. On the other hand, Antimicrobial susceptibility for the bacterial burn isolates revealed that P. aeruginosa was resistant to routine tested antibiotics, like Amoxicillin, Gentamycin and Tobramycin in 100%, While was resistance to Ciprofloxacin 85%, while most P. aeruginosa was sensitive to Ceftizmiden in 50%. Results also showed that Staph. aureus was resistant to Gentamycin, Tobramycin, and Levofloxacin in 50% respectively.Depending on the rate of injury burns microbial and resistance to antibiotics used, p.aeruginosa and Staph.aureus isolated from the burnswere chosen and study their role in the events of infection, experimental burns, and for this purpose has been the use Albino mouse male as a model for a pilot study for pathogenesis and progress associated with the infection of the disease bacterial burns. (33) male mice were divided into tow groups, the first group(24) mice were burned (deep dermal second degree) and infection by 0.2X108 cfu kg of elected isolates, the route of infection was subcutaneously and scratching within burned area. In the second group six were infected without burn, which was considered as positive control, while in the thirdgroup three mice were burned and injected by 0.2ml of normal saline and consider as negative control .the first group was subdivided into two subgroups (12) mices for each burn dominant pathogens, then divided into subgroups each of six mices and following up (3,7, days intervals post infection process). P.aeruginosa burned mice group, shown skin infection more progressive and more severity in comporning with group infected by Staph. aureus, In addition, they were significant increases in the body temperature of all infection burned mice group(p.value)compared with those pre - infection process. Reisolation of bacteria from infection mice organs revealed, that high percentages in experimental p.aeruginosa burned model comparison with Staph. aureus.Diagnosed bacterial isolates by API 20and Vitek compact system, in addition to molecular diagnostics using PCR technique for the detection of 16S rRNA gene, All isolates showed a positive result of this gene. The amplified 16S rRNA gene seguence was compared with the seguence in NCBI seguence database.the bacterial strain identified as P.aeruginosa EPSI. This study reflects the usefulness of sequence analysis of the 16S ribosomal RNA gene in identifying bacteria and determining bacterial diversity. Various techniques that are based on utilizing the 16S rRNA gene are discussed. Of critical importance is the use of massively parallel sequencing to study bacterial diversity. Through massively parallel sequencing which is replacing traditional methods of bacterial identification; various bacterial habitats are surveyed to compile their species compositions.

التوصيف المظهري والجزيئي للانواع البكتيرية المصاحبة لحالات الحروق في محافظة ذي قار == Phenotypic and Genotypic Charactrization of Bacterial species Assocuated with burn infections in ThiQar province

Author name: عبير هادي فرهود الحبيب
Supervisor name: رحمن لعيبي جلاب
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: هدفت الدراسة الحالية الى تشخيص الانواع البكتيرية المصاحبة لحالات الحروق والكشف الجزيئي عن الموروثه 16SrRNA في الانواع البكتيرية المشخصة واستخدام تقنية DNA Sequencing . تناولت الدراسة الحالية فحص ومعاينة 107 مريضا تم اختيارهم بشكل عشوائي (ذكور واناث) تراوحت اعمارهم بين 1 - 45 سنة من الراقدين في مستشفى الحسين التعليمي للمدة من حزيران - 2015 تشرين الاول 2016 اذ اجري الفحص السريري من قبل الطبيب المختص وتشخيص الاصابة على انها حالة حروق مرضية ناتجة عن اسباب مختلفة. جمعت 107 عينة مسحات قطنية Cotton swabs وزرعت على وسط اكار المكونكي ((MacConkey agar واكار الدم ((Blood agar والاكار المغذي ((Nutrient agar. شخصت العزلات عن طريق الاختبارات المظهرية والبايوكيمائية وعامل التشخيص API20E ونظام الفايتك ( (Vitek 2compactفضلا عن التشخيص الجزيئي باستخدام جين.16S rRNA واظهرت نتائج الدراسة الحالية وجود 117 عزلة بكتيرية تفوقت فيها العزلات السالبة لصبغة كرام ((Gram negative بنسبة 93.16% على العزلات الموجبة لصبغة كرام (Gram positive)بنسبة 6,83 وكانت نسب توزيع العزلات البكتيرية المشخصة Pseudomonas aeroginosa 32.47% , Klabseilla pneumonia 21.36% واقل نسبــة عزل كانـت لبكتريا Staphylococcus epidermidis بنسبة عزل 1.7% , في حين كانت نسب العزلات البكتيرية التالية Acinetobacter baumannii , Escherishia coli , . Enterobacter claocae ,Staphylococcus aureus , Burkholderia cepacia, Pruteus mirabillis, , Pantoea agglomerans بنسبة عزل. 12.36% , 11.1% , 5.12% , % 5.12 , 3.41% , 2.56 على التوالي.تناولت الدراسة الحالية بعض الجوانب الوبائية للحالات المصابة التي شملت معلومات عن المرضى المصابين بالحروق التي تضمنت الجنس , العمر , موقع السكن ونوع الحرق. وبينت النتائج ان عدد الحالات المشخصة كاصابات حروق للذكور كانت بنسبة 42.9% بينما كانت نسبة اصابة الاناث 57.%, تم تقسم المرضى الى عدد من المجاميع حسب الفئات العمرية. اعلى نسبة اصابة كانت في الفئة العمرية 9 - 1سنة بنسبة 54.2% وهي الفئة الخاصة بالاطفال تليها الفئة العمرية erishi29 - 20 بنسبة 20.56% واقل نسبة اصابة كانت في الفئة العمرية 49 - 40 بنسبة 4.67% وكانت نسب الفئات العمرية الاتية 19 - 10 , 39 - 30 بنسبة 11.21 , 9.34 على التوالي. اشارت النتائج الى ان حالات الاصابات المشخصة توزعت بنسب متفاوتة فيما يتعلق بنوع الحرق اذ كانت اعلى نسبة اصابة بالحروق بواسطة الماء المغلي Boiled water 38% تليها اصابة الحرق بواسطة لهيب الغاز Fire by gas flame بنسبة 28.9% واقل نسبة اصابة كانت لحروق الصدمة الكهربائية بنسبة 4.67%.اما فيما يتعلق بطبيعة ومكان السكن فقد اظهرت الدراسة ان اعلى نسبة اصابة كانت بين الافراد الذين يسكنون المناطق الريفية بنسبة 84.1% في حين كانت اصابات المركز بنسبة 15.88% . وجدت الدراسة الحالية ان نسبة الاصابة بالحوادث غير المقصودة كانت 86.9% في حين كانت الاصابات المقصودة 13% , لوحظ في الدراسة الحالية ان اعلى الاصابات بالحروق كانت تحدث في فصل الشتاء بنسبة 75.7% مقارنة باصابات الحروق في فصل الصيف 24.29% .اما بالنسبة الى اختبار فحص الحساسية فقد بينت نتائج الدراسة الحالية ان اكثر المضادات الحيوية فعالية ضد كل من البكتريا السالبة لصبغة كرامBacteria Gram negative والموجبة لصبغة كرام Gram positive هي Ceftazidin , Ciprofloxacin,Amicacin .واظهرت الدراسة ان اكثر العزلات مقاومة هي بكتريا P.aeruginosa التي اظهرت مقاومة لكل المضادات الحيوية بنسب متفاوتة. اكدت نتائج الدراسة الجزيئية لتضخيم جين 16SrRNA ان كل العزلات اعطت نتيجة موجبة لهذا الجين وان حجم الجين لكل الانواع البكتيرية كان 1500pb كذلك اكدت النتائج ان الحصول على500Pb من DNA Sequencing للجين 16SrRNA كافية لتشخيص الانواع البكتيرية كلا على حدة . ايضا تم مقارنة جميــــع نتائج DNA Sequencing للانواع البكتيرية (Pseudomonas aeruginosa ,Escherishi coli, Enterobacter cloaca , Proteus mirabillis, , Klebsiella pneumonia Staphylococcus.heamolyticus ,) التي اكدت تشابه نسب تتابع القواعد النيتروجينية مع نسب القواعد النيتروجينية المخزونة في البنك الجيني باستخدام برنامج MEGA6 Moleculas Evolutionary Genetics Analysis)) اكدت نتائج الدراسة الحالية على وجود 21 سلاله جديدة قيد التسجيل واعطيت الرمز TQU1 الى TQU12. | The present study addressed the examination and preview the 107 patients. who were randomly selected (males and females) and who ranged in age from 1 - 45 years old and have fallen asleep at the Hussein Teaching Hospital of the, period from Jon 2015 to November 2016. where he conducted clinical examination by a specialist doctor and diagnosed as a case of burns satisfactory result for different reasons. specimens were cultured on appropriated culture MacConkey agar, Blood agar and Nutrient agar, diagnosed isolates by phenotypic tests, Biochemical test, diagnosis factor API20E system Vitek 2 compact, as well as to molecular diagnostics, During this study, In total 117 bacterial isolates.109 isolates were Gram - negative bacilli and 8 were Gram - positive cocci. he distribution of bacterial isolates diagnosed Pseudomonas aeroginosa 32.47%, Klabseilla pneumonia 21.36% and the lowest rate was to isolate the bacteria by isolating Staph.epedermidis 1.7%, while the following bacterial isolates Acinetobacter baumennii, Escherishia coli, Enterobacter claocae, Staphylococcus .aueus, Burkholderia cepesa, Pruteus mirabillsi, Pantoea agglomerans by isolating : 12 : 82% , 11.1% , 5.12% 5.12% 3.41% 2.56 respectively. The present study addressed some of the epidemiological aspect of infected cases burns,which included information on patients with with burns, which included sex, age, residence location (place), burning type. The results showed that (42.9%) isolates were males and (57.%) were female.The most frequent patient were belonged to the 0 - 9 years old (54.2%) which is for children. 29 - 20 followed by isolation of 22 20.56% and less isolating rate was in the 49 - 40 age group. Created results also cases of infections diagnosed were distributed to varying degrees with respect to the type of cremation, where the highest rate of injury burns Boiled water 38%, followed by the burning by flames gas Fire 28.9%,Fire by gasoline flames (19.6%),Hot liquids (8.41%) and Electrical Shock(4.67%). As for the nature and place of residence have study found that the highest proportion of cases were among individuals who live in rural areas increased by 90 injured 84.1% while the injuries the center has reached 17 cases of burn injury by 10.88% ,Frequency of the manners of burning based on intention or accidentally was as follow : (86.95) of patients were burned accidentally and (13%) were intenion. Present results showed that the most frequent burning (75.7%)was occurred during winter. The lowest frequency (24.2%)was belonged to summer .So the most common time of burning of present was during winter. As for the screening test sensitivity was shown the results of the current study that the most effective antibiotics against both Gram - negative bacilli bacteria and Gram - positive cocci were Ceftazidin, Ciprofloxacin, Amicacin while most of isolates showed high resistance to Ticarkcillin, Gentamicin, Impenem. Where the highest proportion of resistant bacteria P.aeruginosa which showed high resistance to all antibiotics in different proportions. It showed the results of the molecular study to amplify the gene 16SrRNA that all isolates gave a positive result and it's within the range 1400pb - 1500 pb. Also it has been compared to the results of all the DNA Squencing bacterial species (P.aeruginosa, E.coli, E.cloaca, A.baumennii, P.mirabili), which confirmed its similarity ratios nitrogenous bases relay with nitrogenous bases ratios stored in the gene bank

التوصيف الجزيئي لبعض الفطريات الانتهازية المعزولة من المرضى الضعاف مناعيا واختبار حساسيتها تجاه مستخلص الثوم == Molecular Identification of Some Opportunistic Fungi Isolated from Immunocompromised Patients and Their Testing Sensitivity Against Extract of Garlic ( Allium sativum)

Author name: هند حبيب عبد الامير الفرطوسي
Supervisor name: ياس خضير عباس | ميثاق ستار عبود
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: تضمنت الدراسة الحالية العزل والتوصيف الجزيئي والمظهري لبعض الفطريات الانتهازية من القناة التنفسية السفلى للمرضى الضعاف مناعيا المراجعين لقسم الاورام في مستشفى الحبوبي والمراجعين للعيادة الاستشارية للامراض الصدرية في محافظة ذي قار (جنوب العراق) حيث تم جمع 130عينة قشع sputum للفترة من 1/10/2015 الى 1/5/ 2016 . بلغ عدد العينات الموجبة للزرع 85عينة وبنسبة 65.38% , اذ شكل عدد عينات الاناث الموجبة 21 عينة وبنسبة 46.67 % , اما عدد عينات الذكور الموجبة فقد بلغ 64 عينة وبنسبة 75.29% . ظهرت اعلى نسبة عزل عند الفئة العمرية 61 - 70 سنة وبنسبة 32.94 % بعدها الفئة العمرية 31 - 40 سنة وبنسبة 15.25 % . وتم الحصول على 106 عزلة فطرية . باستخدام طرق التشخيص المظهرية والجزيئية, بينت هذه الدراسة ان اعلى نسبة ظهور للفطريات المعزولة تمثلت بجنس spp. Candidaثم جنس Aspergillus spp.تلاه جنس Penicillium spp. بالنسب المئوية 49.41% 38.32 % 21.17 % وبالترتيب . كما ظهرت Candida albicans ثم Aspergillus flavus بالنسب المئوية 36.47 % , 12.94% بالترتيب, كما تم تسجيل 15 عزلة من الفطريات المعزولة في البنك الجيني الدولي . شملت الدراسة استخدام اربعة مذيبات عضوية وهي الايثانول, الاسيتون, الميثانول والماء بحيث كان قد استخدم من المذيبات الثلاثة غير الماء التراكيز 50%, 70% فضلا عن التركيز المطلق للحصول على مستخلص الثوم. اختبرت اربعة تراكيز من كل مذيب ضد 11 نوعا من الفطريات المعزولة, واظهر المستخلص المائي كفاءة جيدة في تثبيط جميع انواع الفطريات المدروسة بالمقارنة مع المذيبات العضوية الاخرى اذ بلغ اعلى معدل تثبيط عند التركيز400 مايكروغرام\مل بحيث ابدى الفطر Penicillium notatum تاثرا كبيرا للمستخلص المائي الذي بلغ اعلى معدل تثبيط له (60 مليمتر) عند التركيز المذكور . حددت الفعالية الحيوية لكل مذيب ضد كل الفطريات المدروسة وتم مقارنة المستخلص مع ثلاثة مضادات فطرية Miconazole وNystatin وAmphtericin B واظهر المضادان الاول والثاني تاثيرات متباينة اما المضاد الثالث فلم يظهر اي تاثير على الفطريات المختبرة . تم تحديد السمية الخلوية للمستخلص المائي الخام باستخدام كريات الدم الحمر ولم يتسبب في اي تحلل دموي. كذلك تضمنت الدراسة الكشف عن المركبات التي يحتويها المستخلص المائي للثوم باستخدام الكشوفات النوعية وتقنية كروماتوغرافيا الغاز GC فكشفت تلك الطرائق وتقنية كروماتوكرافيا الغاز بان المستخلص يحتوي على القلويدات والفينولات والفلافونويدات والتربينويدات والتانينات والكاروتينات بالاضافة الى وجود الاحماض الامينية ومركبات كبريتية اهمها الاليسين allicin واخرى اروماتية | The current study included isolation and molecular and phenotypic identification of some the opportunistic fungi from the lower respiratory tract of the immunocompromised patients in the Department of Oncology and Thoracic Consultant Clinic of Alhaboobi hospital at Thi - Qar Province ( South of Iraq). The specimens of sputum were 130 which collected during 1/ October /2015 until 1/May/2016. The study showed that 85 of the collected specimens appeared as positive results which were 65.38% of the ratio in which the females ones were 21 (46.67%) specimens while 64 (75.29%) for males. In addition that, the highest ratio of the positive results was recorded in the ages of 61 - 70 years, followed by 31 - 40 years which were 32.94% and 15.25%, respectively. Totally, 106 isolates of the isolated opportunistic fungi appeared from the positive specimens. Through using morphological methods and molecular techniques, this study showed the first rank of the isolated fungi was Candida spp followed by Aspergillus spp, and Penicillium spp which possessed ratios of 49.41%, 38.32% and 21.17, respectively. According to the species, the first ratio of the isolation was Candida albicans followed by Aspergillus falvus, as 36.47%, 12.94%, respectively. Fifteen fungal isolates of the present study were recorded in the Gene Bank of NCBI. In this study, the extracts of Allium sativum were separately obtained by using four organic solvents (ethanol, acetone, methanol and water) and three concentrations (50%, 70% and absolute) of each solvents except water. Four concentrations of each extract were tested against 11 species of the isolated fungi in which the water extract gave the best value of the bioactivity including highest inhibitory value (60 mm) against Penicillium notatum by using 400µg\ml compared with the extracts of ethanol, acetone, and methanol. The bioactivity of Allium sativum extracts of each solvent was done against the tested fungi in comparison with Amphotericin B, Nystatin and Miconazole in which two of them produced the different bioactivities while Miconazole had no effect. The blood cytotoxicity of the crude water extract was performed against red blood cells and it did not appear any hemolysis. The chemical tests and Gas Chromatography (GC) revealed that the water extract contains alkaloids, phenols, flavonoids, terpenoids, tannins and carotenes in addition to sulfur formations, such as allicin as well as aromatic compounds.

التحري الجزيئي والوبائي لطفيلي المتحولة Entamoeba Spp. في الاطفال المصابين بالاسهال في محافظة ذي قار == Molecular and Epidemiological investigation of the Parasite Entamoeba Spp. In children with diarrhea in Thi Qar Province

Author name: منار كريم كاظم السعيدي
Supervisor name: فاضل عباس منشد العبادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: شملت الدراسة الحالية فحص 3909 عينة غائط للاطفال دون سن 12 سنة الذين يعانون من الاسهال ويشكون من الام بطنية والمراجعين لمستشفى بنت الهدى التعليمي ومستشفى الشهيد محمد الموسوي والمراكز الصحية والعيادات الخارجية في محافظة ذي قار خلال المدة من شهر تشرين الاول 2015 لغاية شهر ايلول 2016 تضمنت الدراسة ثلاثة محاور رئيسة . المحور الاول هو تشخيص جنس المتحولة مجهريا باستعمال المسحة المباشرة الرطبة وطريقة التركيز وذلك للكشف عن الاطوار المتغذية والمتكيسه للطفيلي واظهرت اصابة كلية بجنس المتحولة Entamoeba spp. بنسبة 26.86% واختيرت 80 عينة من العينات الموجبة مجهريا لتاكيد التشخيص باستعمال الفحص الجزيئي PCR بواسطة الجين 18S rRNAفسجلت الدراسة اصابة كلية بطفيلي المتحولة بنسبة 35% ، اظهرت نتائج الفحص باستعمال طريقة Nested PCR لتمييز الانواع (Species Specific) اصابة بطفيلي المتحولة الحالة للنسج Entamoeba histolytica بنسبة 39.29% وبطفيلي المتحولة المتغايرة Entamoeba dispar بنسبة 39.29% . فحصت العينات الموجبة (28) للPCR باستعمال Real - Time PCR وبوجود المجسان Probes المجس Histolytica - 96 T الخاص بالمتحولة الحالة للنسج والمجس Dispar - 96 T الخاص بالمتحولة المتغايرة وذلك لتاكيد التشخيص وتمايز الانواع فثبت وجود المتحولة الحالة للنسج باعلى نسبة 53.57% والمتحولة المتغايرة بنسبة 39.29% والانواع الاخرى لجنس المتحولة بنسبة 7.14% وبفارق معنوي بين الطريقتين الجزيئيتين مع وجود تاثير معنوي لنوع الطفيلي في احداث الاصابة . اظهرت الدراسة اصابة مفرد لطفيلي E. histolytica بنسبة 45.50% ولطفيلي E.dispar بنسبة 27.20% فيما سجلت اصابة مختلطة بنسبة 27.30% كما وسجلت وجود كريات الدم الحمر في 80% من العينات الموجبة لوجود E. histolytica وبنسبة 54.55% من العينات الموجبة لوجود E.dispar . تضمن المحور الثاني دراسة انتشار الاصابة بجنس المتحولة من خلال دراسة علاقة الاصابة مع بعض العوامل الوبائية ، منها فصول السنة التي اظهرت وجود فروق معنوية P≤0.05 فكان فصل الصيف اعلى الفصول من حيث نسبة الاصابة 37.06% وادناها في فصل الشتاء بلغت 14.14% ، كذلك لم تبين النتائج وجود فروق معنوية للاصابة فيما يخص الفئات العمرية فكانت النسب اعلاها بين الاطفال دون (3) سنوات فبلغت 30.50% ادناها بين الاطفال في الفئة الاعلى من (10) سنوات بلغت 17.13% ، لم تسجل الدراسة اية فروق معنوية في نسبة الاصابة بالطفيلي بحسب الجنس فسجلت نسبة 26.87% و26.85% للاناث والذكور على التوالي، في حين اظهرت الدراسة وجود فروق معنوية ملحوظة فيما يخص المستوى المعاشي فكانت نسبة الاصابة لدى الاطفال الذين ينتمون للعائلات ذات الدخل المحدود هي الاعلى 37.98% واقلها للذين ينتمون للعائلات ذات الدخل فوق الجيد وبلغت 10.11% كما سجلت النتائج وجود فروق معنوية في نسب الاصابة حسب المستوى التعليمي للابوين فكانت نسبة الاصابة عالية لدى الاطفال الذين المستوى العلمي لذويهم دون الابتدائية مقارنة بذوي الاطفال من حملة الشهادات الجامعية وبلغت النسب 36.85% و8.55% على التوالي فيما يخص عدد افراد العائلة كانت نسبة الاصابة عالية وبفارق معنوي لدى الاطفال الذين ينتمون لعائلات تتكون اكثر من (10) افراد واقلها للذين ينتمون لعائلات التي يبلغ عددها (5 - 3) افراد بنسب بلغت 36.95% و8.54% على التوالي . تضمن المحور الثالث دراسة بعض المعايير الدمية ومعرفة التغير في نسبة هذه المعايير لدى الاطفال المصابين بالطفيلي عند مقارنتها بالمعايير الدمية للاطفال الاصحاء وتم ذلك باستعمال جهاز Coulter Counter . جمعت 150 عينة دم من الاطفال المصابين بطفيلي المتحولة و50 عينة دم من الاطفال الذين يمثلون مجموعة السيطرة الاصحاء وتبين وجود فروق معنوية واضحة في نسبة خضاب الدم 10.16%وحجم كريات الدم الحمر المضغوط 34.7% حيث لوحظ انخفاضهما في مجموعة المصابين مقارنة بالقيم في مجموعة السيطرة التي بلغت 12.34 و39.10 على التوالي. سجلت نتائج العد الكلي لخلايا الدم البيض وجود ارتفاع معنوي في الاطفال المصابين مقارنة بمجموعة الاطفال الاصحاء وبلغت 11.3، 8.37 على التوالي وفيما يخص العد التفريقي لها فقد بينت النتائج وجود ازدياد بفارق معنوي في عدد الخلايا العدلة 6.92 و4.03 والخلايا الحمضة 0.61 و0.17 والخلايا الوحيدة 1.19 و0.93 على التوالي ولوحظ كذلك وجود انخفاضا معنويا في عد الخلايا اللمفاوية في الاطفال المصابين مقارنة بقيمتها لدى اطفال مجموعة السيطرة التي بلغت 2.19 و2.76 على التوالي | The current study included examination of 3909 stool samples from under 12 years old children who visited Bint Al - Huda hospital, Martyr Mohammad Al - Mosawi hospital, medical centres and outpatient clinics in Thiqar governorate during the period from October 2015 until September 2016. All those individuals were suffering from diarrhea and abdominal pain. This study included the microscopic identification of Entamoeba Spp. using direct wet amount and concentration techniques in order to diagnose both Trophozoite and cyst stages of the parasite. The result revealed that 1050 (26.86%) of the collected samples were infected with Entamoeba Spp. Out of these infected samples, 80 were randomly selected for further confirmation by conventional PCR and 18S rRNA. Using this method, 28 (35%) was recorded to have Entamoeba Spp. Positive samples from conventional PCR were further examined by Nested PCR for species specific identification. The result of this method showed the presence of two species, E. histolytica (39.29%) and E. dispar (39.29%).Positive samples for molecular examination (28) were then examined by Real time PCR in order to verify and confirm the molecular results. Two probes were used, Histolytica - 96 Tfor E. histolytica and Dispar - 96 T for E. dispar. A significant difference was observed between two methods. E. histolytica was found to be higher percentage followed by E. dispar and then Entamoeba Spp., 53.57%, 39.29% and 7.14% respectively. The Real time PCR reported mono - infection with E. histolytica in 45.5% and with E. dispar in 27.2%, whereas reported mixed infections by both parasites in 27.3%. Red blood cells (RBCs) were detected in 80% E. histolytica positive samples and in 54.55% E. dispar positive ones.The environmental factors and their correlation with Entamoeba Spp. prevalence were also included in this study. The infection prevalence based on the seasonal variation showed significant differences P≤ 0.05. Summer recorded the highest infection prevalence whereas the lowest was in the winter, 37.06% and 14.14% respectively. Regarding the infection in age groups, the highest percentage (30.5%) was recorded in under 3 years old group and the lowest (17.13%) was found in above 10 years old one. However, no significant difference P≤ 0.05 was observed between these age groups. Furthermore, no significant differences were recorded betweenpatient’s sex. Entamoeba Spp. infection (37.98%) appeared to be higher in children who belong to families with low standard of living compared to those who are from families that earning a higher income (10.11%). A Similar pattern was found in Entamoeba Spp. prevalence based on the number of the patient’s family members. Higher prevalence and significant difference were reported (39.95%) in patients who belong high family member (above 10), compared to those from 3 - 5family member (8.54%).One hundred and fifty blood samples from infected children with Entamoeba Spp. and fifty samples from healthy children as a control were collected during the present study in order to observe the change in blood picture and parameters between two groups. Significant differences were reported in blood hemoglobin (10.16%) and volume of packed RBCs (34.7%) in the infected group compared to 12.34% and 39.10% in the control group. Total leukocyte count was found to be significantly higher in the infected group (11.3) compared to the individuals in the control group (3.37). Differential leukocyte count also showed significant differences in Neutrophils (6.92%, 4.03), eosinophils (0.61, 0.17), monocytes (1.19,0.93) and lymphocytes (2.19, 2.76) in infected and control groups respectively.

تاثير الاصابة التجريبية بطفيلي المقوسة الكوندية Toxoplasma gondii وبعض الادوية على خصوبة الجرذان المختبرية Rattus norvegicus == Effect of experimental Toxoplasma gondii infection and some drugs on fertility rats Rattus norvegicus

Author name: زهراء سعدون هادي الغزي
Supervisor name: فاضل عباس منشد العبادي | علي اسماعيل عبيد السنافي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: This study included 3 stages. In the first stage, Toxoplasma gondii was isolated from aborted women placenta who visited Bent Al - Huda hospital and Al - Shatra hospital after confirmation of the prescence of parasite by impression smear method. Then parasite uspension injected 0.3 ml which contain 100 cyst intraperitoneally (174 rat) to 87 male and 87 female rats, inaddition to control group 30 rat (15 male and 15 female). After 10 days of injection, diagnosed the acute infection by Real - Time Poplymerase Chain Reaction(Real - Time PCR) and showed 97.70% (170 infected rat and 4 noninfected rat) , in male 96.55% (84 infected rat and 3 noninfected) ,in female 98.85% (86 infected rat and 1 noninfected) . After 2months of infection 84 animals (42 males and 42 females)were sacrificed, dissected (brain, testis and ovary ) were isolated to confirm the occurance of chronic infection with the using Real - Time PCR. The incidence of infection was 100% in brain males and ovary females and recorded 71.43% in testis and 90.48% in brain females. So diagnosed infection by impression smear method, identified the amount of DNA of the parasite T. gondii by using Real - Time PCR. The use of the remaining number of animals within the following experiments.The second stage included effect of experimental Toxoplasmosis infection and some drugs on fertility 168 rat (84 male and 84 female). Animals were subdivided into 6 groups for each group included 14 rat : (First group) non infected treated by DMSO, (Second roup) non infected treated by Sulfadiazine, (Third group) non infected treated by Pyrimethamine, (Fourth group) infected treated by DMSO, (Fifth group) infected treated by Sulfadiazine and (Sixth group) infected treated by Pyrimethamine.The current study results revealed prominent effect of T. gondii infection on the hormonal level in both males and females. T. gondii infection induced significant decline in Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH) and Testosterone levels in nfected male compared with non infected males treated by DMSO. Sulf. and Pyr. treatment significantly elevated the level of these hormones (P <0.01) in infected males compared with fourth group similar to levels its in first group. Toxoplasmosis in female rats significantly decrease FSH, LH, Estrogen and Progesterone levels in compared with first group, while treating of infected females with Sulf. and Pyr. didn,t improve the declined levels of these hormones in infected and noninfected females comparedwith fourth and first group, respectively.The study showed that Toxoplasmosis in male rats induced significant decline in the sperm count in the epididymal cauda, sperm viability and relative weights of male sexual organs with an increase in sperm malformation ratio in infected male compared with first group. Treatment with Sulf. and Pyr. in infected male significanthy elevated the sperm count and viability (P<0.05), compared with fourth group. Sulf. didn,t induced further significant changes, but Pyr. induced further significant increase in sperm malformation ratio in infected (P< 0.01) and non infected (P<0.05) rats. The study also revealed that Toxoplasmosis significantly decrease the relative weight of ovary and uterus compared with first group, while treatment with Sulf. and Pyr. increased the declined relative weight of the ovary and uterus in the infected females.Testicular histological section of Toxoplasmosis infected male rats treatment with DMSO showed vacular degeneration of spermatogonia and spermatocytes. The seminiferous tubules revealed sever degeneration, shrinkal, necrosis, hemorrhage. Seminiferous tubules lumen contained little amount of sperm with appearance of gaint cell, polymorphonuclear leukocytes and exfoliated cellular debris. Metamorphosis and disintegration of the tissue between the seminiferous tubules was also observed compared to the first group that showed complex histologically intact while testes sections of infected rats treatment Pyr. and Sulf. showed improvement by removing histocompatibility damage caused by the parasite infection.The epididymal sections of T. gondii infected rats treatment with DMSO showed infiltration of mononuclear and multinucleated gaint cell. Sections also showed hyperplasia of duct lining and contained tissue cyst of T. gondii. All these changes were improved in infected males treated by Sulf. and Pyr. Ovary sections of the infected females treatment with DMSO compared with first group revealed decreased primary and secondary follicle increased follicle atresia. Histological sections of ovary females showed in the fifth and sixth group to increase the number of primary and secondary follicles and decreased number of follicle atresia compared overian sections for the fourth group. Uterine sections of the infected females treatment with DMSO showed hypertrophy, increase endometrial thickness and myometerium, increase polymorphonuclear cell infiltration, fewer glands and congestion of blood vessels compared with first group. Histological sections for ovary females of the fifth and sixth group showed changes but less severe than is present in the fourth group.In third stage from studying Toxoplasmosis on fertility parameters of male and female rats, 84 rat(42 male and 42 female) which showed the results of the current study, when paired fertilized healthy females by males is infected and infected of T.gondii treatment with Sulf. , Pyr. and DMSO, and inversion, there is the effect of the parasite and used drugs the percentage of getting pregnant and pregnancy size and rate of embryos weight and placental weight and increase the proportion of fetal resorption and the proportion of early loss of embryos in fertilized healthy females by males five groups compared to the first group.Based on that conclude from the study that the infection of parasite T. gondii had negative effects on the fertility of male and female, and that all of thePyr. and Sulf. were not honest is the other of the impact on reproductive functions.

دراسة جزيئية ومناعية لالتهاب الكبد الفيروسي نمط جـ لمرضى الثلاسيميا في محافظة النجف الاشرف - العراق == Molecular and immunological study for Hepatitis C virus in Thalassemia patients in AL - Najaf AL - Ashraf / Iraq

Author name: ناديه حبيب سرحان الشيباني
Supervisor name: موسى نعمة مزهر | جميل جري يوسف
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Master
University: University of Kufa - College Of Education For Girls
Language: Arabic
University location: Najaf
First pages:
Abstract: This study was conducted to detect the infection percentage of Hepatitis C Virus (HCV) in the samples of thalassemic patients in AL - Najaf Governorate.About 130 suspected cases attending AL - Zahra'a hospital for children and delivery in Al - Najaf city during period from 1st of November (2014) to 28thof April (2015).Only 69 samples were carried out in this study .The practical side for this study followes three main paths : 1 - The Molecular identification of the presence of Ribonuclic acid of Hepatitis C virus in Thalassemic patients by using RT - PCR.2 - Using the Enzyme Linkedimmunosorbant assay (ELISA - III)technique to determinate immunological markers anti - HCV IgG and interleukine - 28B (IL - 28B).3 - Using theCYANHemato devicefor the purpose of counting blood cells which is called Coulter - Method to give a Complete blood count(CBC).The overall results obtained in this study were as follows : - Out of 69 samples,25(36%) samples of thalassemic patients were infected with Hepatitis C virus, 16 male and 9 female ,their age range from (4 - 43) years ,while 44(64%) samplesof thalassemic patients were withoutHepatitis C virus infection , 22 male and 22 female, their age range from (3 - 35) years . - The result showed that 25samples seropositive to anti - HCV IgG in all age groups where the age group (10 - 20) years old has thehighest percentage among other age groups. - The result of the examination of HCV - RNA Eleven of (25) HCV seropositive patients were positive to RT - PCR technique. - The result of this study showed that level of Interleukin - 28Bin thalassemic patients with HCV was high (mean or significant) (12733.33) pg/ml as compared with thalassemic patients without HCV (270.0463) pg/ml and control(12.1267)pg/ml. - The result also has revealed that the hematological parameters of white blood cells (WBCs),monocytespercentage (MON) and lymphocytes percentage (LYM) significantly have increased (P<0.05) in Hepatitis C patients compared to those of control group. - Granulocytes(GRA) showed a significant decrease (P<0.05) in percentage in Hepatitis C patients compared to those of control groups.

دراســـــة بعض المعايـــــير الجزيئيـــــة والمناعيـــــة لبكتيريا Staphylococcus aureus المقاومة للمثيسيلين والمعزولة محليا من الاصابات الجلدية المكتسبة مجتمعيا

Author name: هدى سعد عزاوي
Supervisor name: سلمان عزيز | سميرة كاظم
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University of Kufa - College Of Education For Girls
Language: Arabic
University location: Najaf
Key words:
  • المكورات العنقودية الذهبية
  • الخراج ونضح الجروح
  • الاصابات الجلدية
First pages:
Abstract: تعد البكتيريا العنقودية الذهبية المقاومة للمثيسيلين المكتسبة مجتمعيا من الكائنات المجهرية المرضية التي تسبب طيفا واسعا من الاصابات في مختلف بقاع العالم في اماكن مختلفة من جسم الانسان اذ اقترنت امراضيتها مع اصابات الجلد والانسجة الرخوة . تهدف هذه الدراسة الى التعرف على مدى انتشار هذه البكتيريا في حالات اصابة انسجة الجلد المختلفة ,واجراء تقييم جزيئي لهذه البكتيريا المعزولة من حالات مرضية مختلفة , وتقييم مستوى بعض الحركيات الخلوية في مصول المرضى . وقد اشتملت على 169 عينة اخذت من المرضى المصابين بالتهابات الجلد المختلفة كـ الدمامل والخراج الجلدي والجروح والقدم المصابة بالقرحة لدى مرضى السكري وعدوى الحروق والحصف (القوباء) واخذت من المرضى الذين يراجعون العيادات الخارجية , والمراكز الصحية , وكذلك العيادات الخاصة, لمدة امتدت اكثر من خمسة اشهر , وللفترة من تشرين الاول 2014 م وحتى اذار2015 م. تضمنت الدراسة الحالية اخذ مسحات من الجلد المصاب بالالتهابات المرضية المختلفة وعينات دم ,من بين 169 عينة ,76 عزلة فقط اثبتت عائديتها الى جنس المكورات العنقودية الذهبية وبنسبة 45% من العزلات الماخوذة من حالات مختلفة لاصابة الجلد , وكان معدل العزل العام للعنقوديات الذهبية من القدم المصابة بالقرح لدى مرضى السكري 5 عزلات بنسبة 33% نسبة العزلات المقاومة للمثيسيلين 20% (عزلة واحدة ),اما المصابين بخراج الجلد والدمامل والقوباء الجلدي فكان معدل عزلها كالاتي (69)18 %,(55)21% , (31)9%,نسبة العزلات المقاومة للمثيسيلين (55.6)10% , (61.9)13%, (11.11)1% على التوالي . ان معدل عزل هذه البكتيريا من عدوى الجروح والحروق فكانت كالاتي (32)11%, (44)12% نسبة العزلات المقاومة للمثيسيلين منها (72.7)8%% (16.6)2, على التوالي . ان العزلات البكتيرية من المكورات العنقودية الذهبية المكتسبة مجتمعيا مقاومة لجميع مضادات البيتالاكتام وغير مقاومة للمضادات الحيوية التي لا تنتمي الى هذه المجموعة. اجري الكشف الوراثي باستعمال تقنية سلسلة تفاعلات البلمرة polymerase chain reaction اذ شخصت العزلات الخمسة والثلاثين باستعمال 3 جينات وهم mec Agene وهو الجين الخاص بالمقاومة للمثيسيلين , وكان عدد العزلات الحاملة لهذا الجين 22 عزلة من اصل 35 عزلة وبنسبة 62% . واخضعت العزلات المقاومة للمثيسيلين الى عملية تعريف للقطعة الكروموسومية النوع الرابعSCCmec IV اذ يعد هذا النوع سمة تفريقية للعزلات البكتيرية من المكورات العنقودية الذهبية المقاومة للمثيسيلين والمكتسبة في المجتمع ، اذ وجدت بنسبة 100 % . كذلك تم التحري عن امكانية تواجد مورثة الذيفان الخارجي Panton Valatine leukocidine ( pvl ) باستعمال تقنية تفاعلات البلمرة PCR اذ شخص في 19 عزلة بكتيرية بنسبة 54% ، اقترن وجود السم بالاخص مع حالات الاصابة بالدمامل والخراج والجروح . اجريت الفحوصات المناعية عند المصابين بالتهاب انسجة الجلد , وذلك من خلال تقييم مستوى بعض الحركيات الخلوية مثل IL - 8 وLTB - 4 باستعمال طريقة الممتز المناعي المرتبط بالانزيم Enzyme linked Immune Sorbant assay وقد لوحظ زيادة معنوية في تراكيزها في مصول المرضى مقارنة بعامل السيطرة . | Community acquired methicillin resistant Staphylococcus aureus is one of the pathogenic micro - organism that cause a wide spectrum of infections in different sites of the human body where their pathogenicity is related to skin and soft tissue infection. This study is aimed to explore the role of this bacteria in different cases of skin infections.The genetic structure of these isolated strains of Staphylococcus aureus was also one of this study targets to evaluate immunological response by measurement of concentration of some cytokines as indicator of immune response. One hundred sixty nine patients with different cases of skin infection were enrolled in this study by taking one skin swab from each patient with different skin infection as Furuncle, boil, wound infection, impetigo and burns infection. All these cases were taken from out - patient clinics and private clinic along 5 months period from October 2014 to march 2015 in AL - Najaf Province , From the 169 swab samples only 76 swabs were shown to be positive Staphylococcus aureus with percent 45%.The percent of isolates of S. aureus from different cases of skin infection (5) isolates from diabetic foot with 20% of them are Methicillin resistant (one isolate) and skin abscess, boil, and impetigo were 18(69%),21(55%),9(31%)from which methicillin resistant were 10(55.6%), 13(61%) and 1(11.11%) respectively Percent of isolates from wound &burns infection were 11(32%) and 12(44%) and methicillin resistant isolates were 8 (72.7%) and 2(16.6%) respectively . The process of diagnosis of methicillin - resistant isolates was performed by using sensitivity test for cifoxitin following (CLSI,2014) instruction . Only 35 isolate showed resistant to methicillin when Cefoxitin disk was used then antibiotic sensitive test for other antibiotic disks done., Isolates of CA - MRSA showed resistant to all β - Lactam antibiotics and also to other drugs that are not belonged to this type of antibiotics. Genetic study was evaluated by using PCR technique to diagnosis the following genes in 35 isolates : mecA gene : which is considered responsible for methicillin resistant ,it was found that only 22 from 35 isolates (62%)carrying the gene. SCCmec IV gene which restricted only to Community acquired isolates of Staphylococci were explored in the isolates which considered a diagnostic character for Community acquired Staphylococci and this gene was found in 100% of isolates . Exotoxin panton - valatine toxin also diagnosed in 19 isolates with percent 54% in isolates of S. aureus using PCR technique. This exotoxin was produced in cases of Furuncle, skin abscess, and wound infection . Immunological test was introduced in this study to evaluate the innate immune response by measuring the concentration of Cytokines IL - 8&LTB4 which showed significant elevation in their concentration in comparison with healthy persons.

دراسة لاعتلال الاعصاب المحيطية عند مرضى الداء السكري من النوع الثاني == Study of peripheral neuropathy in patients with diabetes mellitus type II

Author name: اسراء ماجد نعمة عبيد العيكاشي
Supervisor name: سحر محمود جواد الجمالي | عمار جبار حمد الشمري
General topic: Biology
Specific topic: Zoology
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Biology
Language: Arabic
University location: Najaf
First pages:
Abstract: Diabetes mellitus is one of the most chronic diseases that is widely spread all over the world and affects all age groups. The disease has a vast potential to affect almost every body organ, especially the peripheral nerves, which causes high mortality rates from disease complications. These complications cause damage in the organs of the body including the nerves located in the limbs that generate an intense heat resulting from the destruction of nerve fibers in addition to the acute pain resulting from the effect of diabetes in those nerves. This study was designed to determine the sensitivity of those nerves by displaying the results of nerve conduction study for patients and compared it with their clinical examination to determine the severity of numbness and assess the level of pain in the upper and lower limbs.The study was conducted at the Diabetes and Endocrinology Center in Al - Sader Medical City in Al - Najaf governorate and it included following up the case of (78) patients with diabetic peripheral neuropathy by nerve conduction study. The study was began from 5/9/2014 until 7/4/2015. It included diabetic men and women, and they were divided into three age groups (35 - 68), and the study excluded the patients with peripheral neuropathy but have foot ulceration because the nerve connect system (SUDOSCAN REPORT ) was used in the current study, cannot perform nerve conduction study for patients with foot ulceration also smoking and the patients who taking treatment for diabetic peripheral neuropathy .During this study the clinical examination for patients was conducted and compared it with the nerve conduction study of them , also recording the information were related with the history of patients ,as while asmeasuring the concentrations of urea and creatinine in serum, in addition the study was recognized the effect of diabetes mellitus on the kidneys by estimating the ratio of albumin to creatinine in the urine, or named (ACR). the study compared the glycosylated hemoglobin levels and body mass index with the nerve conduction study results for all patients.The present study has shown a statistical significant difference (P <0.05) between the clinical tests of the patients who were tested by tuning fork as while as nerves test needle test and between nerve conduction study for both sexes also at all age groups included in the study. The results of the study did not show any a statistical significant (P>0.05). difference when compared the results of nerve conduction study among the different age groups for males. For females however, there was a statistical significant difference (P<0.05) amongst the different age groups.The results of the current study did not show a statistical significant difference (P>0.05) as regards the duration of injury for males, while it revealed a statistical significant difference among females (P <0.05), the results of the current study also observed the presence of a statistical significant increase (P<0.05) in the ratio of albumin to creatinine in patients with diabetic peripheral nervous for males and females.When compared the glycosylated hemoglobin (HbA1c) levels over the past three months, there are no a statistical significant differences (P>0.05) for males and females ,while the study revealed no a statistical significant increase (P> 0.05) in the concentrations of urea in males when compared to their nerve conduction study results, as well as theconcentrations of creatinine which did not show a statistical significant difference( P> 0.05) at them.The study was also revealed the presence of a statistical significant difference (P<0.05) in the concentrations of urea in females with diabetic peripheral neuropathic and did not show a statistical significant difference (P> 0.05) in the concentration of creatinine at them. The results of the current study did not show a statistical significant difference (P>0.05) between the body mass index among males and it showed a statistical significant difference among females (P <0.05)with peripheral diabetic neuropathy.The study reached that diabetes, with long periods of infection, has a crucial role in showing and the advancement of the disease for most of the patients, particularly those who lack the suitable amount of control over the percentage of the Glucose in the blood. The study also deducted that the disease is accompanied with kidney failure for most of the studied patients. It was also revealed that the nominees of some of the patients which appeared during their examination via tuning fork as while as nerves test needle was not the cause of their infection with diabetes, therefore, the study recommended the adoption of the results of the diabetic peripheral neuropathy to diagnose and asses the infection with peripheral nervous surround diabetes.

دراسة بعض العوامل البيئية المؤثرة في بعض انواع القشريات في نهر الكوفة == Study Som of Enviromental factors offecting Some Crustacea species in the Kufa river

Author name: ايمان حساني شباط السلامي
Supervisor name: جاسم حميد رحمة الخزاعي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University of Kufa - College Of Education For Girls
Language: Arabic
University location: Najaf
First pages:
Abstract: اجريت هذه الدراسة للتعرف على انواع القشريات وانتشارها في نهر الكوفة , وتاثير بعض العوامل البيئية على تواجدها , اذ جمعت عينات الدراسة شهريا للمدة من اب 2014 لغاية تموز 2015 من ثلاث محطات , الاولى : تقع شمال جسر الامام علي (عليه السلام) والثانية قرب مبزل ميسان والمحطة الثالثة قرب جسر الكوفة القديم ( قريبة من دار ضيافة المحافظة ) , اذ قيست العوامل البيئية الفيزياوية والكيمياوية مثل درجة الحرارة والاس الهيدروجيني والاوكسجين المذاب والمتطلب الحيوي للاوكسجين والتوصيلية الكهربائية والكدرة , والعسرة الكلية وايون الكالسيوم . تراوحت الحدود الدنيا والعليا لحرارة الهواء 14.3) - 42.6 ) مo وحرارة الماء (13 - 30.4) مo والاس الهيدروجيني 6.8) - 9.6 ) والاوكسجين الذائب 2.9) - 9.1 ) ملغم / لتر والمتطلب الحيوي للاوكسجين 0.7) - 4.7 ) ملغم / لتر والتوصيلية الكهربائية 1162) - 2055 ) مايكروسيمنز/ سم والملوحة 0.7) - 1.31 ) جزء بالالف والكدرة 1.4) - 17.5) نفثالين وحدة كدرة والعسرة الكلية 210) - 930 ) ملغم / لتر وايون الكالسيوم 119 ) - 380 ) ملغم / لتر . تم خلال الدراسة الحالية تسجيل جديد لنوعين من القشريات الكبيرة لاول مرة في نهر الكوفة وهما الروبيان النهري Macrobrachium nipponense والسرطان النهري Potamon magnum اللذان يعودان الى رتبة عشارية الاقدام Decapoda , اذ سجل اعلى كثافة للروبيان النهري 63.58 ) ) فرد / م 3 في المحطة الاولى في شهر تشرين الاول واختفى في محطات الدراسة جميعها في شهر كانون الثاني وشباط , بينما سجل اعلى كثافة للسرطان النهري 32.89) ) فرد / م 3 في المحطة الاولى في شهر نيسان . وتم تشخيص 32 وحدة تصنيفية تعود الى رتبتين من القشريات الصغيرة , وهي رتبة Cladocera ورتبة Copepoda اذ وجد ان رتبة Cladocera هي الاكثر وفرة وغزارة في محطات الدراسة اذ تضم خمسة وعشرون نوعا , بينمراتبة Copepoda تضم سبعة انواع.تم تشخيص ثمانية اجناس من رتبة متفرعة اللوامس Cladocera وهي Bosmina , Daphnia , Ceriodaphnia , Simocephalus , Alona , Chydorus , Macrothrix وMonia , في جميع محطات الدراسة , ومن الاجناس التي سجلت اعلى كثافة هي جنس Bosmina وDaphnia بينما سجل الجنسين Macrothrix وMonia اقل كثافة اذ اختفت في معظم اشهر الدراسة وفي جميع محطات الدراسة. وتم تشخيص اربعة اجناس من رتبة مجذافية الاقدام Copepoda وهي Cyclops , Diaptomus , Microcyclops , Macrocyclops , ومن الاجناس التي سجلت اعلى كثافة هو جنس Cyclops ومن الاجناس التي سجلت اقل كثافة هو جنس Diaptomus . وتم تسجيل جديد لاحدى عشر نوع من القشريات الصغيرة Microcrustacea لاول مرة في نهر الكوفة تعود لرتبتين هما Cladocera وCopepoda وهذه الانواع هي umholtzil Daphnia , Daphnia galeata , Daphnia magna , Daphnia retrocurva , Simocephalus sexpinosus , Alona affinis , Chydorus gibbus , Monia micrura , Cyclops strenuous , Macrocyclops albidus , Microcyclops sp . , اظهرت نتائج التحليل الاحصائي وجود علاقة ارتباط بين كثافة القشريات والعوامل الفيزياوية والكيمياوية المقاسة شهريا ولمدة عام , كذلك لوحظ وجود فروق معنوية بين اشهر ومحطات الدراسة بالنسبة لكثافة القشريات والعوامل الفيزياوية والكيمياوية عند مستوى P ≤ 0.05 . | This study was conducted to identify the types of Crustaceans and distribution in Al - Kufa River and the effect of some environmental factors on their presence , Study samples were collected monthly for the period from August 2014 to July 2015 of three stations, the first station is located north bridge Imam Ali , the second near the Missan trocar and the third station near old Kufa bridge . The Environmental factors, were measured ( physical and chemical) such as temperature , pH , dissolved oxygen, , electrical conductivity, salinity , turbidity ,total hardness (T . H) and calcium ion. Lower and upper limits for air temperature ranged from (14.3 - 42.6) Co and water temperature (13 - 30.4) C0 , pH (6.8 - 9.6) , dissolved oxygen (2.9 - 9.1) mg / L , the biological oxygen demand (BOD5) (0.7 - 4.7) mg / L), electrical conductivity (1162 - 2055) µs/ cm and salinity (0.7 - 1.31) ppt , turbidity (1.4 - 17.5) NTU, total hardness (210 - 930) mg / L and calcium ion ( 119 - 380) mg / L. During the current study, a new record of two species of Macrocrustaceans for the first time in AL - Kufa River Macrobrachium nipponense and river crab Potamon magnum which they return to the Order Decapoda, The highest density was recorded for the M. nipponense (63.58) individual / m 3 in the first station in October month and disappeared in the study, all stations at January and February, months , while the highest density of river crab P. magnum record (32.89) individual / m 3 in the first station in April month. in this study diagnosed 32 taxonomic unit return to Microcrustacea : Cladocera and Copepoda orders , also cladocera reverded where found that the most abundant and the abundance of plants in the study are Cladocera rank as comprising twenty - five type, while Copepoda rank of seven . Diagnosed eight genus belong to the Cladocera such as Bosmina, Daphnia, Ceriodaphnia, Simocephalus, Alona, Chydorus, Macrothrix, Monia, in all study stations, The genera Bosmina and Daphnia recorded high density while Macrothrix and Monia recorded low density which disappeared in the most study month in all study stations. Also four genera of the Copepoda were diagnose such as Cyclops, Diaptomus, Microcyclops and Macrocyclops, and genus Cyclops record high density while the genus Diaptomus recorded low density. eleven species of Microcrustacea were recorded for the first time in AL - Kufa River belonging to the Cladocera and Copepoda orders and these species are Daphnia umholtzil, Daphnia galeata, Daphnia magna, Daphnia retrocurva, Simocephalus sexpinosus, Alona affinis, Chydorus gibbus, Monia micrura , Cyclops strenuous, Macrocyclops albidus, and Microcyclops sp. Statistical analysis showed a correlation relationship between the density of Crustaceans and the physical and chemical factors measured per month for one year, also noted that there were significant differences between months and study stations for the intensity of Crustaceans and the physical and chemical factors at P ≤ 0.05

دراسة تاثـير المستخلصات المائيـة لنباتي الليمـون الحامـض والسعـد في ذكور الجرذان البيض السليمة والمصابة تجريبيا بداء السكري == Studying the Effect of Aqueous Extract of Citrus limon and Cyperus rotandus in alloxan induced in Diabetic Male Albino Rats

Author name: زهراء كريـم عبد الله حسن الميالي
Supervisor name: زينب شنيور مهـدي الطرفـي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University of Kufa - College Of Education For Girls
Language: Arabic
University location: Najaf
First pages:
Abstract: داء السكري هو اضطراب ايضي مزمن يتصف بارتفاع سكر الدم مع اضطراب في ايض الكاربوهيدرات والدهون والبروتين نتيجة لخلل في افراز الانسولين من قبل البنكرياس ، او تاثيره الحيوي اوكليهما، يشكل هذا الداء تهديد خطير ومتزايد على حياة الكثير من الناس ويعد المشكلة الرئيسية التي تودي بحياة الملايين، وما للنباتات الطبية اثر مهم في صحة الانسان كونها مصدرا مهما للعديد من الادوية والمستحضرات الطبية , وتكمن الفائدة الطبية لهذه النباتات في قابليتها على انتاج العديد من المركبات الكيميائية ذات الخصائص الصيدلانية التي تدخل كمادة اولية او عوامل مساعدة في صناعة الادوية ولكثرة استخدام الاعشاب (النباتات الطبية) في الوقت الراهن بوصفها الطب البديل جاءت هذه الدراسة . اجريت هذه الدراسة الحالية في كلية التربية للبنات / جامعة الكوفة للمدة من 2014/10/1 م ولغاية 2015/5/30 م وتضمنت الدراسة تحضير المستخلص المائي الحارلكلا النباتين (بذور الليمون الحامض Citrus limon ودرنات السعد (Cyperus rotundusوقد تضمنت الدراسة متابعة (42) حيوان من ذكور الجرذان البيض ، وتم تقسيمها الى سبعة مجاميع تضمنت كل مجموعة ستة ذكور ، اذ جرعت المجموعة الاولى فمويا بمحلول الملحي الفسيولوجي حسب وزن الجسم واعتبرت كمجموعة سيطرة (سالبة) ، اما المجموعة الثانية فقد حقنت تحت الجلد بالالوكسان (1 مل) واعتبرت كمجموعة سيطرة مصابة (موجبة)، اما المجموعة الثالثة فقد جرعت فمويا بالمستخلص المائي الحار لبذور الليمون الحامض بتركيز (500ملغم/كغم) بتركيز (750ملغم/كغم) وحسب وزن الجسم ، والمجموعة الرابعة جرعت فمويا بالمستخلص المائي الحار لدرنات السعد حسب وزن الجسم ، والمجوعة الخامسة فحقنت بالالوكسان تحت الجلد ومن ثم جرعت فمويا المستخلص المائي الحار لبذور الليمون الحامض بتركيز (500ملغم/كغم) وحسب وزن الجسم، والمجموعة السادسة التي حقنت بالالوكسان تحت الجلد ومن ثم تم تجريعها فمويا المستخلص المائي الحار لدرنات السعد بتركيز (750ملغم/كغم) حسب وزن الجسم ، واخيرا المجموعة السابعة التي حقنت بالالوكسان تحت الجلد ومن ثم جرعت فمويا بعقار الاميرال (Glimepiride) بتركيز (0.1) ، علما ان التجريع قد استمر لمدة ثلاثين يوما كاملا وبواقع مرة واحدة يوميا .وقد اظهرت نتائج الدراسة ما يلي :  حصول انخفاض معنوي (P< 0.05) في وزن الجسم واوزان الاعضاء (البنكرياس والكبد والكلية) لدى مجموعة السيطرة الموجبة(المحقونة بالالوكسان) عند مقارنتها بالمجاميع : السيطرة السالبة والمجموعتين المعاملتين بالمستخلص المائي الحار لبذور الليمون الحامض ودرنات السعد على التوالي والمجموعة المعاملة بالالوكسان وعقار الاميرال وكذلك المجموعتين المعاملتين بالالوكسان ومستخلص النباتين المذكورين على التوالي، وعلى النقيض من ذلك فقد اظهر وزن الجسم وكذلك اوزان الاعضاء ارتفاعا معنويا (P< 0.05) لدى المجموعتين المصابتين تجريبيا بداء السكري والمعاملتين بمستخلص النباتين كلا على حدا عندما تقارن بمجموعة السيطرة الموجبة.  وجود انخفاض معنوي (P< 0.05) في التعداد الكلي لخلايا الدم البيض وكريات الدم الحمر وتركيز الهيموغلوبين والصفيحات الدموية عند الحيوانات المصابة تجريبيا بداء السكري عند مقارنتها مع المجاميع : السيطرة السالبة والمجموعتين المعاملتين بالمستخلص المائي الحار لبذور الليمون الحامض ودرنات السعد على التوالي والمجموعة المعاملة بالالوكسان وعقار الاميرال وكذلك المجموعتين المعاملتين بالالوكسان ومستخلص النباتين المذكورين على التوالي ، على العكس من ذلك حصول ارتفاع معنوي (P< 0.05) في مجموعة الحيوانات السليمة لدى المجموعتين المصابتين تجريبيا بداء السكري والمعاملتين بمستخلص النباتين كلا على حدا عندما تقارن بمجموعة السيطرة الموجبة . حصول ارتفاع معنوي (P< 0.05) في مستويات الكلوكوز ، الكولسترول الكلي الكليسيريدات الثلاثية ، والبروتينات الدهنية واطئة الكثافة والواطئة الكثافة جدا وحصول انخفاض معنوي (P< 0.05) في مستوى البروتينات الدهنية عالية الكثافة لدى مجموعة السيطرة الموجبة عند مقارنتها مع المجاميع : السيطرة السالبة والمجموعتين المعاملتين بالمستخلص المائي الحار لبذور الليمون الحامض ودرنات السعد على التوالي والمجموعة المعاملة بالالوكسان وعقار الاميرال وكذلك المجموعتين المعاملتين بالالوكسان ومستخلص النباتين المذكورين على التوالي ، وكذلك حصول انخفاض معنوي (P< 0.05) في المعايير المذكورة اعلاه وارتفاع في البروتينات الدهنية عالية الكثافة في مجموعة الحيوانات السليمة ولدى المجموعتين المصابتين تجريبيا بداء السكري والمعاملتين بمستخلص النباتين كلا على حدا عندما تقارن بمجموعة السيطرة الموجبة . وحصول ارتفاع معنوي (P< 0.05) في فعالية الانزيمات الكبدية ALT, AST وALp والبلروبين لدى مجموعة السيطرة الموجبة عند مقارنتها مع المجاميع : السيطرة السالبة والمجموعتين المعاملتين بالمستخلص المائي الحار لبذور الليمون الحامض ودرنات السعد على التوالي والمجموعة المعاملة بالالوكسان وعقار الاميرال وكذلك المجموعتين المعاملتين بالالوكسان ومستخلص النباتين المذكورين على التوالي ، وحصول انخفاض معنوي (P< 0.05) في فعالية الانزيمات اعلاه في مجموعة الحيوانات السليمة ولدى المجموعتين المصابتين تجريبيا بداء السكري والمعاملتين بمستخلص النباتين كلا على حدا عندما تقارن بمجموعة السيطرة الموجبة . حصول انخفاض معنوي (P< 0.05)في مستوى البروتين الكلي والالبومين والكلوبيولين لدى مجموعة السيطرة الموجبة عند مقارنتها مع المجاميع : السيطرة السالبة والمجموعتين المعاملتين بالمستخلص المائي الحار لبذور الليمون الحامض ودرنات السعد على التوالي والمجموعة المعاملة بالالوكسان وعقار الاميرال وكذلك المجموعتين المعاملتين بالالوكسان ومستخلص النباتين المذكورين على التوالي ، وحصول ارتفاع معنوي(P< 0.05) في في مجموعة الحيوانات السليمة ولدى المجموعتين المصابتين تجريبيا بداء السكري والمعاملتين بمستخلص النباتين كلا على حدا عندما تقارن بمجموعة السيطرة الموجبة . اشارت نتائج الدراسة النسجية للكبد حصول تغير نسجي للكبد في حالة ارتفاع السكر بالدم تمثل بحصول توسع بسيط بالجيبانيات ،واحتقان دموي مع حدوث تنخر للخلايا الكبدية ونزف فيها ، وظهور مناطق التهابية عدة واستسقاء مائي وبعد معاملة المجموعة المصابة بالمستخلص المائي لكلا النباتين لوحظ رجوع الشكل الطبيعي للخلايا الكبدية . اشارت نتائج الدارسة النسجية للكلية حصول تغير نسيجي فيها في حالة ارتفاع الكلوكوز بالدم والمتمثل بخروج الانوية من الكبيبة الكلوية وتجمعها في اماكن متفرقة خارج الكبيبة وكذلك وجود نزف وتحطم في جدران الكبيبة والاوعية الدموية الموجودة ضمنها وتحطم واضح للقشرة الكلوية وعند معالجة الحيوانات المصابة بالمستخلص المائي لكلا النباتين لوحظ رجوع الشكل الطبيعي للكلية . اشارت نتائج الدارسة النسجية للبنكرياس حصول تغير في التركيب النسيجي للبنكرياس في حالة الاصابة بالسكري نتيجة الحقن بمادة الالوكسان تتمثل بوجود النزف الحاد في جميع مناطقها وحصول توسع وتحطم في الخلايا وعدم انتظام اشكالها وانحلال الانوية وخروجها من اماكنها الصحيحة ولكن عند معالجة الحيوانات المصابة بالمستخلص المائي لكلا النباتين لوحظ رجوع الشكل الطبيعي للبنكرياس.وقد سجلت النتائج جميعا بشكل عام تاثيرا افضل للمستخلص النباتي لبذور الليمون الحامض قياسا بدرنات السعد والدرنات افضل من العقار في تخفيض سكر الدم اضافة الى تحسين الاضطرابات الايضية المتسببة عن داء السكري المستحدث بعقار الالوكسان . | Diabetes Mellitus is a metabolic disorder characterized by hyperglycemia due to a defect in Insulin secretion from the pancreas, insulin action or both, with disturbances in (carbohydrates, lipid and protein) metabolic due to a defect in Insulin secretion, Insulin action or both. This disease is a serious and growing threat to the lives of many people , it isthe main problem which cause death to millions,The medical plants have important impact on human health as an important source of many medicines and medical products. The medical advantage of these plants lies in their ability to produce many of the characteristics that the pharmaceutical intervention have as a primary material or contributing factors in the pharmaceutical industry chemical compounds. The frequent use of herbs (medical plants) at the present time as alternative medicine, as came in this study.This study was conducted at the Faculty of Education /University of Kufa during the period from2/10/ 2014 to 30/5/ 2015. The study included the preparation of aqueous extractsofCitrus lemonseeds and Cyperus rotundus tuber. Also the study includedthe use ofaqueous extractsofCitrus lemon at concentration (500) mg/kg andCyperus rotundus at concentration (750) mg/kgineach dosageaccording to the body weight of the albino rats (Ratus ratus). The study includes (42) white malerats divided into seven groups with six rat in each group and they were injected according to their weight in the following way : the first group was orally dosed with physiological saline and it was considered as a control(negative), the second group was injected with alloxan (1ml.) and were considered as infected control group (positive). The third group were also dosed orally with decocted extract of citrus lemon seeds in (500mg/kg). The fourth group was dosed orally with decocted extract of Cyperus rotundus (750mg/kg) concentration. The fifth group was first hypodermically injected with alloxan and then orally dosed with decocted extract of citrus lemon seeds in (500mg/kg) concentration. The sixth group was first hypodermically injected with alloxan and then orally dosed with decocted extract of Cyperus rotundus (750mg/kg) concentration. The seventh group was first hypodermically injected with alloxan and then orally dosed with Glimepiride with (0.1) concentration. The dosing continued for 30 days.  A significant decrease (P< 0.05) was shown in total body weight and organ weight such as pancreas, liver and kidney in uninfected control(Negative) when compared with other groups : in the two groups that were treated with hot aqueous extract of Citrus lemon seeds ad Cyperusrotundus tuber respectively, also,the group was treated with Amyral and the group that injected with alloxan and that treated with hot aqueous extract of Citrus lemon seeds and Cyperusrotundus tuber, we alsofound a significant increase (P< 0.05) in the above criteria in the two groups that were injected by alloxan and treated by two extracts when compared with the positive control group.  A significant decrease (P< 0.05) was shown in the total WBC and RBC count , and the hemoglobin concentration platelets in uninfected control group when compared with other groups : in the two groups that were treated with hot aqueous extract of Citrus lemon seeds and Cyperusrotundus tuber, respectively, also the group was treat with Amyral and the group that were injected with alloxan and that which was treated with hot aqueous extracts of Citrus lemon seeds and Cyperusrotundus tuber, also a significant increase (P< 0.05) was shown in the above criteriain diabeticanimalsgroup compared with healthy control group, we also found a significant increase (P< 0.05) was shown in the two groups that were injected by alloxan and treated by two extracts when compared with positive control group.  A significant increase (P< 0.05) was clear in the levels of glucose , total cholesterol, triglyceride, low density lipoprotein , very low density lipoprotein and a significant decrease (P< 0.05) in high density lipoprotein in uninfected control group when compared with other groups : in the two groups that treated with hot aqueous extract of Citrus lemon seeds and Cyperusrotundus tuber, respectively.Also the group was treated with Amyral and the group that injected with alloxan and that treated with hot aqueous extract of Citrus lemon seeds ad Cyperusrotundus tuber, a significant decrease (P< 0.05) was clear in the above criteria and significant increasein high density lipoprotein in diabeticanimalsshown in the two groups that were injected by alloxan and treated by two extracts when compared with the positive control group.  A significant increase (P< 0.05) the effectiveness of hepatic enzymes(ALT , AST ,ALP, Bilirubin) in uninfected control group when compared with other groups : in the two groups that were treated with hot aqueous extract of Citrus lemon seeds and Cyperusrotundus tuber, respectively, also the group was treated with Amyral and the group that injected with alloxan and that treated with hot aqueous extract of Citrus lemon seeds and Cyperusrotundus tuber, also a significant decrease (P< 0.05) in the effectiveness of hepatic enzymes in diabeticanimals, a significant decrease (P< 0.05). The effectiveness of the above enzymes in the two groups that were injected by alloxan and treated by two extract when compared with the positive control group.  The presented histological study of the liver refers to the histological changes in the liver in the state of hyperglycemia that is represented by the expansion of sinusoid, blood accumulation, necrosis and bleeding in hepatocyte, presence of many inflammation areas, watery dropsically but when treating diabetic animals by aqueous extract of (Citrus lemon andCyperusrotundus), it wasnoted thatthey returned back to the normal shape of hepatocyte. The histological study of the kidneyrefers to the occurring of the histological changes in the kidney in the state of hyperglycemia that were represented by getting out the nucleus from glomerulous and aggregation in many area out theglomeruli, also, the bleeding or the destruction of glomerulous wall with blood vessels, the present distraction in kidney cortex but when treating diabetic animals with aqueous extract of (Citrus lemon and Cyperusrotundus), it was noted that it returned back to the normal shape of kidney. The histological study of the pancreasrefers to the occurring of the histological changes in infected diabetic animals by (alloxan) which represented an acute bleeding in all regions, destruction and expansion in kidney cells, abnormal shape, nucleus degenerative and leaving the true area but when treating diabetic animals with aqueous extract of (Citrus lemon and Cyperusrotundus), it was noted that it returned back to the normal shape ofpancreas.In conclusion, the results of this study indicate that the Citrus limonseeds and Cyperus rotundus tuberextracts gave good results, even better than the reference drug Amyral for loweringbloodsugar, in addition to improvingmetabolic disorderscaused byalloxan induced-diabetes

دراسة لتكوين الغشاء الحيوي لبكتريا Escherichia coli وProteus mirabilis المعزلة من اصابات المسالك البولية في محافظة النجف الاشرف == Study of Biofilm formation of Escherichia coli and Proteus mirabilis isolated from urinary tract infection in AL - Najaf Al - Ashraf governorate

Author name: رسل عيدان محسن
Supervisor name: سهام جاسم محسن
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Kufa - College Of Education For Girls - Department Of Pathological Analyzes
Language: Arabic
University location: Najaf
First pages:
Abstract: The aim of this study is to inferring the ability of biofilm production and virulence factors which has helped to form it into two types of bacteria P.mirabilis and E.coli. Two hundred and fifty samples have been collected from patients who are suffering Urinary tract infection (UTI) in Teaching Hospital at Al - Najaf Al - Ashraf during the period ( 1/10/2014 - 1/1/ 2015). The samples have has identified by diagnostic methods : culturing , microscopic , biochemical and using VITEK - 2 compact system more sensitively and specifically method.The results have been showed that the isolation ratio was 30 (37.5%) of E.coli, while P.mirabilis was 14(17.5%) also, a five isolates (6.5%) wereEnterobacter cloacae ssp cloacae and only two isolates (2.5%) were Raoultella ornithinolytica from the total number of specimens. The virulence factors and antibiotic sensitivity test of two bacteria : P.mirabilis and E.coli were determined and the more virulence and multidrug resistance were selected.The hemagglutination properties of P.mirabilis which isolated from clinical samples were determined , the reaction with red blood cells of human group O+ was explored. The ratio of Hemagglutination was reached 42.86%, 50%, 7.14% to MSHA, MRHA and NOHA respectively. However, E.coli has been a different results that was reached 30 % , 26.67% and 43.33% from type MSHA, MRHA and NOHA as well. On the other hand, the thin - film production (pellicle) has been formed by 13 isolates of bacteria P.mirabilis and 21 isolates of bacteria E.coli, when it was growth on the nutrient broth in a temperature 37˚C for 24 - 18 hours. Moreover, it has been shown that there is high affinity for adhesion with the epithelium cell in the urinary tract. In addition, the E.coli has proved the higher efficiency than P.mirabilis in adhesion. It was detected ability of bacteria to biofilm production in three methods. The bacteria E.coli have showed their ability on biofilm production where by the rate was (43.3, 52 and60)% on the different growth ways which was the Congo Red, the tube and the plate respectively.The gel electrophoresis has been done on all isolates which was to analyzed on adhesive factors genetically whereby the bacteria P.mirabilis, E.coli have shown ability to gene expression (fimH and afaB) with a rate of (42.86 and 28.57)% and (30 and 20)%, respectively.Also, it has been clarified that the antibiotics ( Ciprofloxacin, Amikacin and Ceftazidime) have an effect on the most virulent bacteria to prevent the formation of biofilm when it is taken a half of minimum inhibitory concentrations. It was found that the minimum inhibitory concentrations were the most efficient in the prevent a biofilm formation in both types of bacteria.It appears to be found that the minimum inhibitory concentrations of these antibiotics were the most efficient in the prevent the formation of biofilm in both types of bacteria. Ciprofloxacin has the high efficiency to prevent the biofilm formation in E.coli while this concentration has no effective on biofilm production from others. It has been found that there is a clear positive relationship between the presence of adhesion gene (fimH , afaB) and the ability to adhesion on the epithelial .The conjugation process was conducted successfully to transfer the adhesion genes between (E.coli and P.mirabilis) which have been considered a donor strains to transfer the genes to the standard strain E.coli HB101 which have been considered a receipt strains whereby have shown ability to adhesion and biofilm formation.

دراسة حيوية وتشخيص جزيئي لداء الاكياس العدرية في المضائف الوسطية اعتمادا على تحليل تتابع جينات rDNA - ITS1 وmtCOX1 في محافظة ذي قار == Biological Study and Molecular Identification on Hydatidosis in Intermediate Hosts Depending on Sequence Analysis for rDNA - ITS1 and mtCOX1 Genes in Thi - Qar Province

Author name: وسام جاسم حنش
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was carried out in Thi - Qar province during the period from beginning of January until the end of December 2015 to investigate the prevalence of infection with hydatidosis in human and other intermediate hosts. The molecular techniques was used to detect the strain of Echinococcus granulosus. The present study diagnosed 58 infection with hydatidosis in different organs such as liver, lungs, kidney and spleen of surgically treated patients in AL - Hussein Imam Teaching Hospital inNassiriyah city, centre of Thi - Qar province. The total number of hydatid cysts was 72 cyst in different organs with total intensity of infection 1.24. The females recorded highest percentage of infection 67.24% than males 32.76 %, and the age group 21 - 30 year showed highest percentage of infection 24.14 % . The lowest percentage recorded in age group more than 60 year 5.17 %.The results of study showed that right lobe of liver was more affected 58.62 % than left lobe 13.79 %. The percentage of left lung infection was 10.34 % while right lung 8.62 %. The total average of fertile cysts in infected organs was 81.82 % and sterile cysts 18.18 %. The highest percentage of infection was recorded in house women 87.18 %. The number of recorded cases in Nassiriyah district was 21 case with percentage of infection 36.21 %.During the same period a total of 1303, 1287, 405, 90, and 294 head from sheep, cattle, buffaloes, camels and goats were examined respectively.The percentage of infection was 7.83 %, 7.69 %, 3.46 % and 2.22 % in sheep, cattle, buffaloes and camels respectively, and no infection was recorded in goats. The intensity of infection was 4.07, 3.27, 1.64 and 2 in sheep, cattle, buffaloes and camels respectively. The results of statistical analysis showed no significant differences (P????0.05) in percentage and intensity of infection.The percentage infection of liver, lungs and both together in sheep was 5.45 %, 1.23 % and 1.15 % respectively, and there was no significant differences in percentage of infection but intensity of infection differed significantly(P????0.05). The percentage of liver, lungs, both together and spleen infection in cattle were 3.11 %, 2.95 % , 1.55 % and 0.08 % respectively while intensity of infection in liver, lungs, both together and spleen was 2.5, 2.18, 7 and 1 respectively. The percentage of liver, lungs and both together infection in buffaloes were 2.22 %, 0.99 % and 0.25 % respectively while intensity of infection in liver, lungs and both together was 1.89, 1 and 2 respectively. No significant differences in percentage and intensity of infection in camels andboth percentage and intensity of infection in liver and lungs were 1.11% and 2 respectively.High significant differences was recorded between percentage infection in males and females of animals. The percentage of infection in females were 15.87 %, 13.42 %, 6.99 % and 3.45 % in sheep, cattle, buffaloes and camels respectively. Highest percentage of infection were 0.72 %, 0.18 % and 0.46 % in males of sheep, cattle and buffaloes respectively. No infection were recorded in males of camels.T he age was effect on percentage of infection and big aging groups recorded in sheep, cattle, buffaloes and camels. The present study recorded significant differences in percentage of fertile hydatid cysts in liver and lungs of animals and highest of percentage of fertility recorded in liver and lungs of sheep 84.21 % and 79.24 % respectively and lower percentage of fertility was in liver of buffaloes 11.11% and liver of cattle 14.70 %. The lower percentage of sterile hydatid cysts was in liver and lungs of sheep 15.79 % and 20.75 % respectively and highest percentage recorded in liver of buffaloes 88.88 % and spleen of cattle 100 %.S t a tistical analysis using of ANOVAs test showed no significant differences in total length, length of blade and width for large and small protoscolices hooks of hydatid cysts isolated from liver and lungs of sheep, human, cattle buffaloes, camels and hooks of sheep which is showed genetic variations (new strain).The PCR - RFLP technique was used in identification of E.granulosus strains infecting human and intermediate hosts in Thi - Qar province. The molecular study is carried out on 75 hydatid cysts collected from human, sheep, cattle, buffaloes and camels. The DNA is extracted from protoscolices of hydatid cysts isolated from infected organs. PCR - RFLP technique showed that sheep strain G1 of E.granulosus is predominant in Thi - Qar province depending on amplification of rDNA - ITS1 gene. The PCR products in all DNA samples showed 1000 bp approximately. Alu1 endonuclease with rDNA - ITS1 demonstrated two bands 800 bp and 200 bp approximately, while Rsa1 endonuclease showed two bands 655 bp and 345 bp approximately which is similar to sheep strain genotype 1.Sequence analysis for rDNA - ITS1 gene showed the presence of sheepstrain genotype 1 which is similar 100 % to sheep strain in gene bank exceptone isolate (sheep liver) was differs from other strains in position ofnucleotide sequence. It was similar 99 % for sequence of sheep strain. Thedata base of this new strain was recorded in gene bank for DNA sequence National Center for Biotechnology Information (NCBI) and European Nucleotide Archive (ENA) for the first time globally under the name Echinococcus granulosus strain WH1AW2 (GenBank : LT547814). Polymerase chain reaction technique and sequence analysis of mtCOX1 gene showed the presence of sheep strain G1 of human, sheep, cattle, buffaloes and camels. The sequence analysis showed the genetic variation in mtCOX1 gene for hydatid cyst in infected sheep represented by point mutation type transition which caused change Adenine to Guanine, and sequence data base of the sample recorded for first time in NCBI and ENA (GenBank : LC203589). Buffaloes strain G3 isolated from human lung recorded for first time in Thi - Qar province depending on comparison of samples sequences with source strains recorded in gene bank (NCBI) using "BLAST". The size of bands for mtCOX1 gene was 450 bp in all samples

استخلاص وتنقية وتشخيص ببتيد كلايكوسيدي من الحبار Loligo sp. ودراسة تاصيرة على خط الخلايا السرطانية Hela Cell Line والطبيعية REF == Extraction , Purification, Characterization Glycosidic Peptide From Loligo sp. and Study Effect On Cancer Cell Line (Hela) and Normal (REF)

Author name: هيفاء عدنان منصور الموسوي
Supervisor name: بلسم انيس مارينا | ضمياء قاسم سكر
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was designated to investigate the capcity of pure Loligo sp extract on two cell lines : - the cancer cell line Human cervix uteri epitheloid carcinoma (Hela) and Rat Embryo Fibroblast cell line (REF) as normal cell line.The study included extraction of tissue (whole body) of Loligo sp by using 30% ammonium sulfate. and chemical group detected in the extract by using qualitative chemical test and the result showed that the crud and pure extract contained alkloides, proteins, carbohdrates, saponines, flavonoids, aldehyde &keton group and phenol group .and the extacte does not contain glycosides.The crude extraction was purified by column chromatography using sephadex G - 25. and the purity of extract detected by using polyacryl amide gel electrophoresis under non denaturated condition. The result showed that the extracted riched with amino acid and some carbohydrate by using high performance liquid chromatography (HPLC) with compared standerd amino acid and suger.Infrared Spectroscopy of purified extract shows that it contain (N - H - C - OH) to emphasize on found amino acid in the extract.The cytotoxicity effect on cell lines study by four concentraction ( 125, 250 ,500 and 1000)ϻg/ml were prepared and tested on cell line with five replicates for each concentraction, the optical density of cell growth read by the Elisa reader 550 nm and use by tetra zolium bromide (MTT). The result for in vitro study showed that all concentraction had high inhibition on tumor cell line and a highly significant inhibition in (Hela) line was recoreded 42.79% at exposure time 48hr in the concentraction 1000 ϻg/ml the effect was dose dependent. The inhibitory effect of extract on Hela cell line higher than in the REF. while it was less effect of extract on Rat Embryo Fibroblast cell line after exposure time 48hr.

مستويات الاديبونكتين والابلين والهرمونات الاخرى والمعايير الدموية اثناء الحمل وفي مرضى متلازمة المبيض متعدد الاكياس == Adiponectin, Apelin and other Hormones Levels and Blood Indices during Pregnancy and in Patients with Polycystic Ovaries Syndrome

Author name: هناء سلمان كاظم
Supervisor name: طه جاسم الطه | علي فالح الاسدي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study aimed to determine the levels of adiponectin and apelin and their relations with all parameters. Investigation in the present study included; hormones, lipid profile, liver enzymes activity and haematological profile in groups of Iraqi Basrah pregnant women during different months of pregnancy and to correlate these levels to maternal age, Body Mass Index, excepted parity and sex of fetuses and correlated with level of adiponectin and apelin hormones only and the results were compared with non - pregnant females as control group. The present study included (180) women ranging from 20 - 40 years old, out of them (70) pregnant and (20) non - pregnant women with polycystic ovary syndrome (PCOs), and (90) females non - pregnant were considered as control group. The pregnant women attended the obsteric units in the general state hospitals in Basra Governorate. They are as follows : Al - Basra General Hospital for Maternity and Childhood, Al - Mawane, Al - Fayha’a and Basra General Hospital which drain most patients from urban and rural areas. None of the selected women suffered from any type of disease. Required data were collected by the researcher depending on direct interview with women before being admitted to the study, using a questionnaire including information on age, parity, month of pregnancy, and geographical area..The Women were divided into two age group (≤ 27 and ≥28 years). Moreover, females were also divided according to BMI into normal (≤ 25), over weight (25 - 30) and Obese (≥30).The month of gestation was determined not only from the date of the women’s last menstruation (LMP) but also according to the ultrasonic reports that pregnant women had. Blood samples (10 ml) were taken from pregnant women at the end of each month of pregnancy and from control group as well as from women with PCOs. Each blood sample was divided into EDTA tube (2 ml) which was used for complete hematological picture study (RBC, Hb, PCV, PLT, WBCs count and WBCs differential count) and the plain tube (8 ml) where the serum was obtained, for biochemical study (Adiponectin, Apelin, testosterone, Progesterone, estradiol, follicular stimulating hormone, luteinizing hormone, insulin, lipid profile and liver enzymes).The results indicated that maternal age had no significant effect on the level of adiponectin, apelin, FSH and LH hormones, while the study showed a significantly effect on level of progesterone, estradiol, testosterone and insulin hormones between pregnant and non - pregnant women. Adiponectin levels decreased in pregnant and non - pregnant females with increased body mass index whereas apelin, estradiol, progesterone and insulin levels increased. When the comparison between pregnant and non - pregnant women was done, a decrease in adiponectin and apelin was noticed. Accompanied by an increase in the level of estradiol, testosterone, progesterone and insulin hormones in pregnant women compared to non - pregnant women.Women with PCOs showed low levels of both Adiponectin and apelin with high levels of Testosterone and Insulin.Results also revealed that age had no effect on total cholesterol, triglycerides, and HDL, in both pregnant and non - pregnant females, but when the BMI was taken in consideration, high levels of these parameters were found in - group of BMI (obese). In addition, the present study showed that age had no effect on liver enzymes (AST and ALT) while ALP showed that the behavior increased up as a different high body mass index in pregnant women, in comparison with the control group. However, ALP activity also increased in high BMI group.Hematological study showed that age had no effect on RBC, Platelets, and hematocrit. Similar results were found when BMI was taken in consideration. Total WBCs showed a non - significant increase in pregnant women as compared with the control group. Adiponectin levels were significantly higher in pregnant females carrying female fetuses compared to those with male fetuses, while the other hormone apelin did not differ between the two groups. The Result of the present study revealed that adiponectin was significantly decreased as apelin was significantly increased with the increased parity.

دراسة مسحية للبكتريا المرتبطة بالتهاب الزائدة الدودية وتحديد الحالة النسجية للمصابين في محافظة البصرة == Survey of bacteria associated with appendicitis and determination of Histological patient state in the province of Basrah

Author name: هدى عبد الرحيم مذكور
Supervisor name: محمد عبد الامام احمد | نوري حنون جاسم
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study has been done to determined pathogenic bacteria that associated with appendicitis .This study includes ninety samples of removal appendix taken form patients who cleared diagnosed as appendicitis infection by specialised doctors in general Basrah hospital and Al - Sadir teaching hospital for the period between September - 2013 and June - 2014.Distribution of appendicitis appear in all age groups, 10 - 20 year and 20 - 30 year were the most infected, as well as the infection appear in both gender, but rate of infection in male was relatively higher than female 68(59%), 47(41%) respectively. The ratio of the city's population has overcome the rural population 96(83.5%),19 (16.5%) respectively. The percentage of samples that gave positive culture was 80( 88.9%), while 10 (11.1%) of these samples negative culture. The study reveals 15 different bacterial isolation, the most common bacteria was Escherichia coli 80(44.9%) while other species was appears in less percentage Shigella dysenteria 14(7.9%), Salmonella enterica typhi 10( 6.5 %), Pseudomonas aeruginosa 8( 5.6 %) , Klebseilla Pneumonia 6(4.3%) , Klebseilla oxytoca 3(1.7%), Morganella morganai 7 ( 9.3 %) Neisseria spp. 6 ( 9.5%) , Enterobacter cloaca 4 ( 2.2 %) ,Serreatia spp. 3 )7.1 %(, Citrobacter ferundii 2 (7.7%) Proteus vulgaris 7) 6.5 %( Staphylococcus spp. 14(1.3%), Streptococcus spp. 72(5.1 %) and Bacillus subtilis 8)5.6 %(. Laboratory diagnosis for blood samples included estimated of total WBCs and found that (31%) of patients have natural WBCs values while the other patients have high values.Antibiotic sensitivity test to E.coli isolates towards 20 antibiotics because she is the most common bacteria showed that all isolates were resisted for most antibiotics which used in test especially for β - lactam group, and the isolates of E.coli were multi resistance for antibiotics.Identified minimum inhibitory concentrations MIC for some antibiotics The results showed that E.coli bacterial isolates's 30 isolation have shown resistance to anti AMO as MIC values ranged from 128 - 1024 Mcgm / ml while the MIC for Anti AMP values between 4 - 128 Mcgm / ml, As for antibiotics and Cefixim , Ceftriaxone, Cefotaxime, and it was her MIC values ranging between (1 - 128) Mcgm / ml Plasmid profile of E.coli isolates investigated to study the correlation between plasmid profile and antibiotic resisitant marker and results from agarose gel electrophoresis revealed that all E.coli isolates contain one plasmid band.This study includes the detection of some genes that encode to beta - lactamase enzymes in E.coli which were responsible about multi antibiotic resistanc and these genes loaded on plasmid DNA for ten isolaes and found that 5(50%) from isolates have blaTEM gene and 5 )40%( have blaCTX gene and 1(10%) have blaSHV gene. This study also considered note the general appearance of appendix samples , some of them are enlarged and surrounded by vesicles, some with fibrous walls and ulcerated with mixed colors, then examined the histological changes it, the study showed changes in histological structure of the excess was extensively congestion of blood vessels, veins in serosa and subserosal layers and increased the amount of diffuse lymphoid tissue in the layers of the appendix walls.

دراسة فعالية بعض مستخلصات الطحلب الاخضر Enteromorpha intestinalis (L.,1820 على الاكياس العذرية للمشوكة الحبيبية Echinococcus granulosus (Batsch,1786 وكمضادات لخط الخلايا السرطانية نوع HeLa == Study of the activity of some of the green alga Enteromorpha intestinalis (L.,1820) extracts on Hydatid cysts of Echinococcus granulosus (Batsch,1786) and as anticancer cells type HeLa

Author name: غزوان طالب نوري الجابر
Supervisor name: صبيح هليل المياح | احمد محسن عذبي
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was conducted in order to evaluate the activity of green alga Enteromorpha intestinalis extracts (methanol and hexane extracts) on growth and development of hydatid cysts of Echinococcus granulosus in vitro and in vivo by using mice type Mus musculus Balb\c that in comparing with albendazole drug. Anti - cancer activity was also evaluated in vitro for both algal extracts by using HeLa cells. Chemical compositions of algal extracts were analyzed by using Gas chromatography - mass spectroscopy (Gc - mass)technique . The hydatid cysts were collected from livers and lungs of infected sheep which slaughtered in Basrah abattoir. While, The algae specimens were collected from Abu Sokhir marshland area /north of Basra. The results revealed that E.intestinalis chemical compounds are carbohydrate , alkaloids, phenols , saponins , triterpenes and sterols compounds. In vitro study showed that the 1 mg / mouse weight of albendazole drug was able to reduce the percentage of protoscolices activity to zero after the third day of treatment. While, the dose 6 98 mg / kg of methanol extract inhibited all protoscolices after five days of treatment, followed by the dose 804 mg / kg of hexan extract that inhibited all protoscolices after six days of treatment.In vivo study revealed that the methanol extract (698 mg / kg) treatment was the best in reducing the weights of organs significantly in comparison with the positive control group it was 2.192 , 0.192 , 0.434 and 0.282 g of liver, spleen, lung and kidney respectively. The methanol extract (698 mg / kg) treatment lowered the average number of hydatid cysts (2.6) more than other extracts treatments , then followed by hexane extract (804 mg / kg) treatment it was 2.8 cyst .This study has identified that the liver enzymes Aspartate transaminase (ALT) and Alanine transaminase (AST) values in the serum of positive control group were higher significantly than negative control group the value of the enzyme ALT was 79 IU / l and the value of the enzyme AST was 118.4 IU / l.All methanolic extracts groups showed a preference in enzyme's values compared with other treatments groups , the value of ALT enzyme of 698 , 688 and 678 mg / kg of methanol extract groups were 46.8 , 45.8 and 44.6 IU / l respectively, while all hexane extracts groups showed a preference in AST enzyme values compared to the other treatments groups, the value of AST enzyme of 804 , 794 and 784 mg / kg of hexane extract groups were 73.6 , 70.2 and 70.4 IU / l respectively. Found a significant increase in the number of white blood cells WBCs in a positive control group ( 4.6 × 013μ), compared with a negative control group and the other groups, While it has been found significant decrease in the Hb (5..8deciliter) and Packed cell volume (PCV) (31..%) values in positive control group as compared with control negative group. The study found that the number of white blood cells WBCs n the methanolic extract group (678 mg / kg) was 4.06 × 103 microliter was as same as in the negative control group, In contrast, hexane extract group (804 mg/ kg) has did not significant difference with negative control group in the Hb and PCV were 11.5 g / dl and 36.8% respectively .Histological study confirmed that there are the histological changes in positive control group included congestion , bleeding , degeneration , infiltration of inflammatory cells and hyperplasia. Whereas the histological changes in group treated with albendazole were bleeding, infiltration of inflammatory cells , atrophy of the glomerulus and fibrosis in some areas . In algal extracts groups the histological changes were infiltration of inflammatory cells, bleeding and congestion.The Gc mass results revealed that the ethanol extract contains loliolide , ethyl stearate , palmitic Acid , ethyl palmitate , phytol , ethyl oleate , ethylhexyladipate and squalene compounds. While, hexane extract has N, Ndimethyltetradecylamine , diisobutyl phthalate , palmitic Acid , ethyl palmitate and ethylhexyladipate compounds.Anti - cancer study showed the algal extracts have an inhibition activity against cancer cells, The half maximal inhibitory concentration (IC50) value of the methanol extract was 79.08 μ/ ml, While, the IC50 for hexane extract was 156.3 μ/ ml.

دراسة تشريحية تصنيفية لبعض من نباتات العائلة السوسبية Euphorbiaceae في العراق == Anatomical and Taxomanical study for some plant of Euphorbiaceae family in IRAQ

Author name: امل علي ياسين الحسن
General topic: Biology
Specific topic: Plant
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was conducted to investigate several taxonomic aspects which comprised the micro - morphological studies of epidermal layer of leaves, pollen grains, fruits and seeds.In addition to that, the anatomical and molecular studies for 12 species of Euphorbiaceae family which belonged to five genera AndrachneL. , Chrozophora L., Euphorbia L, Mercurialis L. and RicinusL., were performed. One representative species for each genuswas selected; in exception 8 species were chosen for the genus Euphorbia..The current results confirmed that the anatomical characters showed valuable taxonomic traits which can evidently be used to distinguish between species. The most prominent trait was mesophylla tissue in leaves which were observed in three categories;1 - Bifacial leaves : - which found in C.tinctoria, Euphorbia sp ., E. milii, E.annua and R.comminus 2 - Uni - facial (heterogenus) leaves : - this category of the mesophyll was detected in two species only A.telephioides and E.prostrata3 - Uni - facial (homogenus) leaves : - this category were noticed in five species; E.denticulata ,E.helioscopia ,E,macrocarpa , E.microsphaeia and E.peplus The second important comparative anatomical character was the Mid rib, which are in four types with several shapes from which discrimination between the species can be made : - 1. Convex - concave mid rib : - that was showed in E.helioscopia, E.macrocarpa, E.milii and M.annua.2. Convex - convex : - This type was found in three species C.tinctoria , Euphorbia sp.and R. comminus3. Flat - Convex : - This type of mid rib was observed in A.telephioides E.microsphaera, E.peplus and E.prostrata4. Flat - Flat : - This type was observed in only one species E.denticulata In stem, the most prominent parts of taxonomic evidence were pith and vascular bundles.In according to the pith, species can be divided into hollow stem (without pith) and solid stem (containing medulla good configuration). Hollow stem was clear in the stems of A.telephioides , E.microsphaera , E.prostrata and M.annua. While, the solid stem wasseen in the remaining species. Vascular bundles were existed in two types; that's connected vascular bundles that's characterized the hollow stems, and separate vascular bundles. In most of the species with the exception of M. annua, stem with scatteredvascular bundles were in cortex As well as, petiole which have features that a clear taxonomy values .either by outline of petiole or its vascular arc.Micro - morphological studies of epidermal layer of leaves showed very important taxonomical value. This layer of leaves reveals variation in shape of ordinary epidermal cell and nature of anticline cell wall, stomata complexes and Epicuticlular wax crystals.Micro - morphological of pollen was taxonomic evidence as well. It can be considered to divide the species of Euphorbiaceae family into two groups based on the number of germination apertures 1 - Hexocorporate : - this type was confined to C.tinctoria2 - Tricolporate all species studied remainingThe sculpture of pollen wall provided beneficial information invested in the species identification.Both seeds and fruits showed a distinctive role in species identification. Micromorophological study of seeds for species which belong to six genera Andrachne , Chrozophora, Euphorbia, Mercurialis , Phyllanthus and Ricinus were achieved by using Scanning Electron Microscope(SEM). The study concentrated on micro - morphological aspect of Caruncle, seed surface ornamentation and the shape of epidermial cell wall. The result revealed that most studied species were with carunclated seeds ,but some specieswere with ecarunclated seeds as in A.telephioides L. ,C. tinctoria( L) Raf , E.prostrata Atin and P. maderaspatensis L Seed surface ornamentation characteristics showed a significant taxonomical value.Furthermore, current result showed that there are a highly variation in shape, size and color of the seeds walls. With respect to the fruits characteristics, which showed variations in number of mercarpi, shape, size, color and surface ornamentation that can be adopted in the separation of the species studied Molecular study provided significant support for phenotypic and anatomical classification and support genetic convergence between the species in Euphorbiacea family. The genus Euphorbia species showed a significant genetically convergence and participated in predecessors monophyletic. However, the genera in Euphorbiacea family under study probably originated and evolved from different ancestors’ polyphyletic.

دراسة تصنيفية وحياتية للديدان الخيطية عائلة Tetrameridae Travassos, 1914 المتطفلة على بعض انواع الطيور في هور السناف محافظة ذي قار == Taxonomic and Biological Studies of Nematodes of the Family Tetrameridae Travassos, 1914 in the Some Birds in Sinaph marsh Thi - Qar province

Author name: نهى جبار عبد الركابي
Supervisor name: باسم ھاشم عبد الله
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: A total of 401 represented by 128 birds of Himantopus himantopus which belong to the family Recurvirostridae , 93 birds of Chettusia leucura which belong to the family charadriidae and 180 birds of Sturnus vulgaris which belong to the family sturnidae at the period from April 2014 to march 2015 in Al - Sanaf marsh , Suq - Al - Shuyukh city southern of the Thi - Qar province,for searching the nematodes which belong to the family Tetrameridae. Four species of nematodes, which belong to the family Tetrameridae, were isolated in the current study. They were T.nouveli, T.dubia, T.spinosa and Microtetrameres inermis. The H. himantopus was infected with the nematode T.nouveli and the prevalence of infection was 25.8% , bird.Furthermore, the Chettusia leucura was infected, the mean intensity was 3.3 worm in each infected birds. The nematode T.dubia was isolated from H. himantopus with the isolated from the Ch. Leucura mean intensity was 1.7.The nematode T.spinosa was isolated from H. himantopus the the mean intensity of infection was 2.3 and it was isolated from the Ch. leucura , the prevalence of infection 4.3% and the mean intensity was 1.8 worm in each infected worm.The record of these three nematode is considering the first record in Iraq.Microtetrameres inermis was isolated from the Sturnus vulgaris, the each infected birds.Himantopus himantopus from April 2014 to March of 2015.This bird was infected with three species from family Tetrameridae, The seasonal variation study of infection prevalence of Tetrameres spp.in, H. himantopus that there was II Summarycomplete seasonal cycle for nematodes T.nouveli, T.dubia , and T.spinosa .The nematode T.nouveli had hightest infection prevalence in summer the decrease in autumn, winter and spring while the infection prevalence of nematode T.dubia was hight in summer and autumn then decrease in winter and spring. The infection prevalence of nematode T.spinosa was generally low with little increase in autumn .The results of the current of the Ch. leucura indicated that this bird was infected with three species which nematodes T.nouveli, T.dubia and T.spinosa and they had seasonal appearance . There was not any significant effect of host sex on prevalence of infection, and it showed decreased in prevalence of infection when the bird's weight was increased.The study of lifecycle of nematodes : T.nouveli ,T.dubia and M. inermis revealed obtainment the larval stages after success the infection experimental of the grasshopper Schistocerca gregaria for the first time the three isolated nematodes from family Tetrameridae in this study which it was T.nouveli , T. dubia and M.inermis and was included the lifecycle of the three species . The differences were including the period of time to produce and developing the larval stages in addition to the differences in their shapes, it was revealed that were clear differences in the structure and their shape of the tip the posterior end of the third larval stage. The end of the third larval stage of the nematode T. nouveli had three caudal papilla and the posterior end of nematode T.dubia had five caudal papilla while the end of the third larval stage of M.inermis did not have any caudal papilla , so we can distinguish these nematode larval stage depended on the prevalence this character in their third larval stage.The experimental infection of the Columba livia domestica with nematode M.inermis was successful.While the experimental infection to the Columba livia domestica with nematode T.nouveli ,T.dubia .was failed The experimental infection of the Gallus domestica with nematodes of Tetrameridae T.nouveli, T.dubia and M.inermis was failed too.

دراسة تشخيصية وحياتية لعلقة المياه العذبة Dina punctata Johansson, 1927 (Erpobdellidae : Hirudinea في اهوار السويب من محافظة البصرة جنوب العراق == Identification and Biological study of the Freshwater Leech Dina punctata Johansson, 1927 (Erpobdellidae : Hirudinea) in AL - Swaib Marshes From Basrah province south of Iraq

Author name: ازهر محمد غالي الخزعلي
General topic: Biology
Specific topic: Zoology - Invertebrates
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The species Dina punctata is recorded for the first time in Iraq (from Al - Swaib marshes). The identification was conducted morphologicaly and molecularly characteristics. The morphological features summarized as : - - The species D. punctata has vermiform body compressed ventrally, ranging in length from 55 - 60 mm and in diameter from 0.5 - 2 mm. The individuals have a large ability for extening. The pointed anterior end carries small anterior sucker which resembles lips surrounding the mouth opining, while the rounded posterior end carries large rounded posterior sucker. - This species is characterized by heterogeneous features such as the color of the body which changes from blackish gray for Adults to dark brown for Juveniles, as well as the number and arrangement of eyes which is in general four pairs arranged as reverse U shape located on the dorsal anterior edge. The first two pairs of eyes are located on the middle line of the body while the third and the fourth pairs are each located on one side of the body behind the anterior pairs. - The body consists of 34 segments, each of them is divided to five secondary annuli. The fifth annuli of each segment is divided into two subsections, thus the main segment becomes consist of six annuli. - The male and female gonopores are open on the ventral surface of the 12th segment, separated from each other by two annuli, the male gonopore is usually larger and clearer and located forward in the middle of the groove which is separate the annuli b2 and a2 respectively. The female gonopore is smaller and located in the back of the male gonopore in the middle of the groove which is separate the b5 and b6 annuli respectively.Because of the instability in some morphological features, the species D.punctata also identified in the present study according to genetic features.During the genetic study, the region of internal transcription space (ITS2) in DNA was isolated from leech specimens by using special primers for region internal transcription space (ITS2), after isolation, the ITS2 genes are amplified.The results of polymerase chain reaction of DNA were obtained of single bands of 580 base pairs molecular weight for all degrees of hybridization. The sequence of each sample after detecting was entered in the database of National Center Biological Information (NCBI) and the result showed that the sequences of all samples are belonging to the species D. punctata. In the field study, monthly samples of leeches D. punctata was collected from the marshes of Al - Swaib on the eastern side of Shatt Al - Arab river inQurna northern Basrah Province during the period from January 2015 to December 2015. A total of 235 individuals and 623 Cocoon were collected during the study period. The highest number was 58 recorded for individuals during May 2015, while being 335 recorded for cocoons during February 2015. On the other hand there is no occurrence recorded during July - August for individuals, and June - November for cocoons.The study investigates the water temperature, salinity and abundance of individuals and cocoons. The statistical analysis showed negative correlation between temperature and abundance of both individuals and cocoons, while the correlation of salinity was slightly positive with abundance of individuals and cocoons.The study of population sizes structure showed that the size class 40 - 50 mm has the highest frequency during the study period compared with the other size classes. On the other hand, the highest percentage of frequency was 66.7%, which also recorded for the size class 40 - 50 mm.Laboratory experiments showed that the members of a species D.punctata able to tolerate saline concentrations between 2 - 6 psu the survival rate 100%, Also, these worms (D. punctata) feed by different ways, it can be considered Predatory Macrophagous and Scavengers, its food materials consist of invertebrate animals such as Oligochaetes, Lumbriculids, Chironomid larvae; fleshy body of some aquatic snails; and on dead fishes. In the laboratory, the leeches fed well on flesh and livers of chicken, sheeps and caws, The laboratory also studied, Three different types of food effect (chicken liver, zooplankton, and animal feed) on the growth of young leech for a period of six weeks.From the field and laboratory observations, the specie D. punctata reproduces sexually by cross fertilization. The male and female gonopores are easily observed in adults which ranging in length from 45 - 60 mm. as well as the sperm sacs on the mid ventral surface of the body during the reproduction season, which extend in our study during two periods : January - May and December 2015. The sexual reproduction processes were studded in detail and the embryonic development in cocoons was divided into five hypothetical stages according to morphological and anatomical changes and these stages are : - 1 - Depositing Cocoon Stage.2 - Primary Cleavage stage.3 - Development and extension.4 - Differentiation of regions stage.5 - Complement and release stage. The histological structure of the body wall, suckers, reproductive organs and digestive tract of adult leeches were investigated. The body wall consists of thick layers of circular, longitudinal and oblique muscles.The structures of the male reproductive system such as genital atrium, sperm sacs and testis were observed. There are two lateral blood sinuses around the mouth cavity in addition to many nerve cells. The posterior sucker is muscular, and the muscle cells are as a dense net in the peripheral part, while are as a loose cells in the middle what give it a sponge appearance, in addition to primitive glomerular cells, nerve cells and mucus cells

معاملة بذور الذرة الصفراء Zea mays L. بحامض السالسياليك واثرها في تحمل الشد المائي المستحث بمادة الكلايكول متعدد الاثلين == The treatment of Corn seeds (Zea mays L.) with salicylic acid and its effects on drought tolerance induced by polyethylene glycol (PEG)

Author name: وسن فوزي عبد الحسین
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - Faculty Of Education
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was conducted in Plant physiology laboratory, department of biology, college of education for pure sciences, University of Basrah from 1/6/2015 till 10/7/2016 in order to evaluate the effect ofpretreatment (soaking) seeds of corn (Zea mays L.) cv. Baghdad 3with salicylic acid (SA) on germination, early seedlings growth and to minimize the effect of water stress induced with poly ethylene glycol (PEG). The study included the following experiments : 1 - Effect of SA and PEG on germination using a hydroponic culture and Hoagland solution in which corn seeds were soaked in three concentrations (0.1,0.3,0.5) mMof SA in addition to control treatment with distilled water for six hours. Water stress were induced with PEG at concentrations of 0 (distilled water),1%,2%,3%,6%,9% and 12%. This experiment indicated that the optimum soaking concentration with SA was 0.5 mM and that there was no germination at 12% PEG.2 - Effect of interaction between corn seeds pretreatment with DW, 0.5mM of SA for six hoursand PEG at all previous concentrations except 12%, on early corn seedlings growth using hydroponic culture and Hoagland nutrient solution, for three weeks.3 - Effect of interaction betweencorn seeds pretreatment with DW, 0.5 mM of SA for six hoursand water stress induced with PEG at 3% and 6% on vegetative growth using pots filled with a mixture of sand and peat moss for three months.The parameters were evaluated after each of the previous experiments, also , Biochemical test . Plant height, leaves number, root length and number, dry and fresh weight for shoot and roots, while the followingparameters were evaluated in corn leaves after pots experiment : 1 - Biochemical tests included : proline and glycine betain concentrations, total carbohydrates, total chlorophyll, sodium and potassium ions concentrations.2 - Leaves anatomical study to compare the effect of PEG inducedwater stress and the role of its interaction with pretreatment with SA on thegrowth and development of internal structure of corn leaves.The following results were estimated : 1 - Germination percentage : increasing water stress in the nutrient media reduced the % germination significantly when compared with control treatment. Seeds pretreatment with SA enhanced the germination percent and the highest percent was at interaction between PEG at 1%, 2% and SA at 0.5 mM.2 - Growth parameters for seedling at hydroponic culture indicated that plant height, fresh and dry weight for shoot and roots have increased significantly when seeds were pretreated with 0.5 Mm SA All theprevious parameters were reduced significantly under PEG induced waterstress especially at 9%. Interaction between SAand PEG caused apositive significant increases in all studied parameters especially atconcentrations less than 6% and 9% PEG.3 - Growth parameters for corn plants grown at pots have showed a significant increase when pretreated with SA compared with that treated with DW. PEG caused a significant decrease in the growth parameters IIIespecially at 6%. Interaction between DW and 6% PEG recorded the lowest growth in all studied parameters. 4 - Biochemical tests : There was a significant increase in total carbohydrates and total chlorophyll content in leaves of corn plants pretreated with SA in compare with that pretreatdwith DW while the content of proline and glycine betaine were higher significantly in leaves pretreated with DW.In case of PEG induced water stress, the highest total carbohydrates content was at 6%, highest chlorophyll content at 3% while proline and glycine betaine highest content were at 6% PEG. 5 - Sodium and Potassium Concentration : The highest potassium concentration was at plant treated with SA while the highest sodium concentration was at plant treated with DW. Increasing PEG concentrations caused a significant increase in potassium concentration and a significant decrease in sodium concentration. Interaction results revealed that the highest potassium and the lowest sodium concentrations were at interaction between SA and PEG at 6%. 6 - Anatomical study results revealed that increasing water stress effected the leaves tissues structures and this were cleared at vascular bundles tissues (xylem and phloem tissues) and bundle sheath. Upper and lower epidermis, polliform cells were also affected by increasing water stress.The anatomy of leaves also showed that there was a positive enhancement in the structure of leaves tissues in the plants grown from pretreated seeds with SA.

دور مستارجات حشرتي الصرصر الامريكي Periplaneta mericana والبعوض Culex pipiens على فرط التحسس من النوع الاول وعلاقتها مع بعض الائل مستضدات معقد التوافق النسيجي من الصنف الثاني

Author name: هدى كاظم كريم الموسوي
Supervisor name: فوزية علي عبد الله | ضياء خليف كريم
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: This study aimed to verify the distribution of Periplaneta americana and Culex pipiens allergy and its association with some HLA class II alleles in asthmatic and allergic patients. 96 healthy (control ) and 96 suspected allergic individuals from the same geographical region ,paired by sex and age were included in this study. Enzyme Linked Immunosorbent Assay test (ELISA) was used to estimate the total and specific IgE antibodies in the sera of studied individuals. The detection of the HLA - DQB1*0602, HLADQB1* 0604 and HLA - DRB1*12 was performed by Polymerase Chain Reaction (PCR).The results of total IgE based ELISA which was conducted on 96 suspected allergic and24 healthy (control ) individuals revealed that there was extremely significant statistical association (P<0.0001) for total IgE concentration with being allergic or non allergic status of individuals. The overall frequency of higher total IgE antibody concentration ( IgE>200IU/ml) was observed in 72.9% of allergic patients and lower serum total IgE levels (IgE<200IU/ml) were associated with 87.5% of healthy individuals. According to the specific IgE based ELISA results the effect of allergic patients age on the IgE seropositivity against cockroach allergens was not considered to be significant(p>0.05),but the effect of sex was statistically significant)P<0.05(.There was no significant effect( p>0.05)for patients age and sex on the distribution of IgE antibodies against mosquito allergens. Out of 96 allergic patients, 59 (61.5%)and 62(64.6%)showed positive IgE antibody response against cockroach and mosquito allergens respectively. Concerning the PCR results the overall frequency of HLA - DQB1*0602 and HLA - DQB1*0604 alleles was %35.6 and %15.3 of cockroach allergic patients respectively and the HLA - DRB1*12 allele was not observed in these patients..Out of 62 mosquito allergic patients, 8(%12.9)showed HLA - DQB1 * 0602 positive results while HLA - DQB1*0604 allele was observed in 4(%6.5)patients and the HLA - DRB1*12 allele was not observed in these patients.The difference in the genotypig results was extremely highly significant(χ2 : 0.030;17.486; degrees of freedom(DF) : 3;p - value=0.00056136) between cockroach and mosquito allergic patients .The association between the presence HLA - DQB1 *0602 , HLA - DQB1 * 0604 and HLA - DRB1*12 and total IgE based ELISA results in allergic patients and healthy individuals revealed that the percentage of HLA - DQB1 * 0602, HLA - DQB1 * 0604 and HLA - DRB1*12 alleles presence in patients having total IgE lower total IgE )IgE<200 IU/ml) was higher than that of high )IgE>200 IU/ml) total IgE patients where HLA - DQB1*0602 appeared in high percentage(69.2%) followed by percentage (15.4 %( of HLA - DQB1* 0604 presence. . The relationship of HLA - DQB1and HLADRB1 alleles with the results of total IgE based ELISA was statistically significant in the least(χ2 : 60.756 and the degree of freedom (DF) : 5;p= P<0.00001) and greatest(χ2 : 11.806 ; the degree of freedom (DF) : 5 ; p = 0.037544) than 200 IU / ml, values .Depending on the association between the presence of HLADQB1* 0602 ,HLA - DQB1*0604 and HLA - DRB1*12 and positive or negative specific results of cockroach or mosquito allergens specific IgE based ELISA results in allergic patients revealed that the percentage of HLA - DQB*0602,HLA - DQB1*0604 and HLA - DRB1*12 alleles presence in cockroach allergic patients having positive results were higher than those ratios that have been observed in patients with negative ELISA results where HLA - DQB1*0602 appeared in high percentage)35.6%( followed by percentage( 15.3% )of HLA - DQB1*0604 . The relationship of HLADQB1and HLA - DRB1 alleles with the results of specific IgE based ELISA was statistically significant (χ2 : 10.412; DF : 3; p - value =0.01536989).Incontrast the percentage of HLA - DQB1*0602,HLADQB1*0604 and HLA - DRB1*12 alleles presence in mosquito allergic patients was found to be higher in negative ELISA results patients also the relationship of these HLA - DQB1 alleles with mosquito allergen specific IgE based ELISA results was not considared statistically significant (χ2 : 3.115 ;DF : 3 and p= 0.37423205).However HLA - DQB1 * 0602 allele was found in the highest percentage (20.6%), followed by allele HLA - DQB1 * 0604 (8.8%).Concerning the presence of HLA - DQB1*0602 ,HLA - DQB1*0604 andHLA - DRB1*12 in cockroach or mosquito allergic patients and healthy individuals,the HLA - DRB1*12 and HLA - DQB1*0602 allels were not found in healthy individuals.The genotyping results difference between cockroach allergic patients and healthy individuals was considered statistically extremely significant (χ2 : 74.485 ;DF : 5 and p =0) as well as the difference in the results of the genotyping of mosquitoes allergic patients and healthy people was statistically with very high significant (χ2 : 23.342 ; DF : 5 and p = 0.00029035).
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