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الكشف عن جينات البيتالاكتاميز واسعة الطيف لبكتريا الكلبسيلا المعزولة من عينات سريرية وبيئية في محافظة ذي - قار == Detection of ESBLs in Klebsiella spp. Isolated from Clinical and Environmental Samples at Thi - Qar Province

Author name: زينب محمد عيدي اللامي
Supervisor name: يحيى عبد الرضا عباس
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Thi-Qar - College Of Science
Language: English
University location: Dhi Qar
First pages:
Abstract: This study aimed for isolation and identification of Klebsiella spp.from clinical and environmental samples and detection the prevalence of some extended spectrum β - lactamases (ESBLs) genes (bla CTX - M, bla CTXM1) in Klebsiella spp. Samples collected were included clinical samples ( Burns , wounds , urinary tract infections, respiratory tract infections, otitis media and vaginal infections ) and hospitals environments. A total of 554 (468 clinical and 86 environmental samples) during the period from October 2013 to March 2014 were collected. Growth on blood agar, MacConkey agar and E.M.B agar was identified by cultural, morphological and biochemical tests and confermated by API 20 E system which revealed that : 45 ∕ 554 ( 8,1%) gave positive growth for Klebsiella spp. as following : 2 ∕ 48 (4.1 %) fromburns , 7∕ 40 (17.5 %) from wounds, 29 ∕315 ( 9.2%) from urinary tract infections, 4 ∕ 25 (16%) from respiratory tract infections, 3 ∕86 (3,4%) from hospitals environments.All the 45 isolates of Klebsiella spp. were screened for their antibiotic resistance against 15 antibiotics of different classes using Kirby - Bauer disk diffusion method. The results showed that all the tested isolates were resistant to at least 6 antibiotics of which they were tested, hence the isolates were considered to be multidrug resistant.The results showed that all of the isolates were found to be resistant to β - lactam antibiotic (ampicillin and amoxicillin) Disk approximation test and not production of ESBLs by these isolates.Combined disk diffusion method was used for detection of metallo - β - lactamase - producing isolates , it was emplace each of imipenem and antibiotic with and without EDTA on Mueller - Hinton agar , because EDTA working to inhibit of metallo - β - lactamase . The results showed that there was no effect for EDTA on the diameter of inhibition zone in all isolates.All the 45 isolates were submitted to molecular detection of some ESBLs genes (bla CTX - M , bla CTX - M1) using PCR technique. The results showed that only 28 ( 62,2%) isolates were carried ) blaCTX - M or blaCTXM1) and 21 isolates were carried both genes (blaCTX - M and blaCTXM1).

تاثير حليب الابل في بعض المعايير الفسيولوجية في اناث الجرذان المختبرية Rattus norvegicus المعامله بالبنزين == The Effect of Camel's Milk in Some Physiological Parameters of Female Rats (Rattus norvegicus) Treated with Benzene

Author name: زينب محمد عباس الغزي
Supervisor name: خالد كاطع الفرطوسي | محمد عجة عودة
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Master
University: University of Thi-Qar - College Of Science
Language: English
University location: Dhi Qar
First pages:
Abstract: The present study was conducted in Department of Biology, College of Sciences, University of Thi - Qar, Iraq to investigate the effect of camel's milk on some physiological parameters of female rats (Rattus norvegicus) treated with benzene which included (weight of body and internal organs, hematological and biochemical parameters). The study was included 48 female rats divided into 8 equal groups, 6 for each group, the control group (A) which treated orally with (5 ml /kg) of physiological solution (NaCl 0.9%) for 4 months, camel's milk group (B) which treated orally with (5ml/kg) camel's milk for 4 months, benzene group(1) (C) which injected (I.P.) with (0.2ml/kg) of benzene by two doses for each week for four months, benzene group(2) (D) which injected (I.P.) with (0.4ml/kg) of benzene by two doses for each week for four months, protection group (E) which injected with (0.2ml/kg) of benzene and treated with (5ml/kg) of raw camel's milk for four months, protection group (F) which injected with (0.4ml/kg) of benzene and treated with (5ml/kg) of raw camel's milk for four months, treatment group (G) which injected with (0.2ml/kg) of benzene and after the end of duration of injection treated with (5ml/kg) of raw camel's milk daily for 1 months, treatment group (H) which treated with (0.2ml/kg) of benzene and after the end of duration of injection treated with (5ml/kg) of raw camel's milk daily for one month, and identified the animal weights at the beginning and end of the experiment and after the end of the period experiment all rats were weight organs (heart, liver, kidneys and spleen) and took blood samples to measure the level of malondialdehyde MDA, Ceruloplasmin CP, Total Cholesterol TC, Triglyceride TG, height density lipoproteins HDL, low density lipoproteins LDL, very low density lipoproteins VLDL and the levels of ALT, AST and ALP. The current study showed significant decrease at (P≤ 0.05) in the weight of (body, heart and kidney), and the acidophil, RBCs, SUMMARY

دراسة تشخيصية ووبائية لطفيلي المقوسة الكوندية لطلبة جامعة ذي قار باستعمال تقنيتي الاليزا Toxoplasma gondii وتفاعل سلسلة البلمرة ذو الوقت الحقيقي == Diagnostic and Epidemiological Study of Toxoplasma gondii for Students of Thi - Qar University by ELISA and Real - Time PCR Techniques

Author name: رباب علي شناوه الموسوي
Supervisor name: بسعاد عقرب العبودي | منال بادي التميمي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University of Thi-Qar - College Of Science
Language: English
University location: Dhi Qar
First pages:
Abstract: Toxoplasma gondii is a unique intracellular parasite, which infects a large proportion of the world, population, but clinically uncommonly causes significant disease. The present study was performed for the first time in Thi - Qar province to estimate the prevalence of toxoplasmosis among university students. Blood samples were collected from 319 (111 males and 208 females) apparently healthy students, they have ages between (18 - 42) years attended from different colleges of Thi - Qar university (students of morning and evening study), during the period from October 2013 to April 2014.Enzyme linked Immunosorbant Assay (ELISA), was used to evaluate the presence of anti - Toxoplasma IgM and IgG antibodies and detection of B1 gene of T.gondii DNA by Real - Time Polymerase Chain Reaction (RT - PCR).The results indicated that 21.94% of students were exposed to positive for anti - Toxoplasma antibodies, 5.33% of them had IgM, 11.91% had IgG, and 4.70% had both IgM and IgG. Statistically, they were significant difference between them.There were a significant difference between males and females in total rate but not significant of antibodies pattern in them, which recorded 7.52% of males and 14.42% of females were positive for anti - Toxoplasma antibodies.The study demonstrated that no significant difference in infection percentages between colleges where students studied, although the highest seropositivity students was 47.83% in education sports college whereas it did not score any infection with toxoplasmosis in College of Education for Pure Science .The current study also showed that the highest positive percentage with T.gondii of male students was 28.57% in College of Engineering while the highest percentage with T.gondii of female students was 30.43% in College of sports education.The present study revealed that the acute infection of toxoplasmosis which presented IgM and both IgM and IgG was high at small age youngstudents (18 - 22) years while the chronic infection which presented IgG was high at old students (38 - 42) years.The present study demonstrated that seroprevalence of toxoplasmosis was significantly associated with close contact with cats. Although more students in the positive sample group eating undercooked meat but not significant difference between those 36.13% and other students who did not have this habit 13.5% has been discovered.The results showed the highest positive percentage samples at middle socioeconomic level was 24.10% while the lowest percentage at good socioeconomic level was 19.60% .The results showed that the highest positive percentage samples 31.0% at Refaee district whereas the lowest percentage was 19.78% as noticed at Naserya district.The present study demonstrated that seroprevalence of toxoplasmosis was significantly associated with presence of clinical symptoms (fever, headache, malaise, influenza) that may have a relationship with toxoplasmosis of infected students when compared with not presence clinical symptoms of those (p<0.05) they recorded 18.57% and 81.43% respectively.The present study revealed an association between blood group system and Toxoplasma infection with highest prevalence among samples of blood group A+ 30.65% and lowest prevalence in samples of blood group B+ 17.44% .The current study evolutes the rate of some blood parameters, in infected and non infected students, there was no significant difference observed in mean hemoglobin level (Hb), total count of leukocytes (WBCs), neutrophilis, lymphocytes, monocytes and basophilis numbers in both student . While significant differences were observed in mean of eosinophils number of infected students was more than number of non infected students.Moreover, mean of lymphocytes number recorded significant differences between infected and non infected females only.Besides the serological diagnosis of T.gondii, Real - Time PCR (RT - PCR) technique was used to confirm the infection with T.gondii by detection B1 gene of T.gondii DNA in the blood of students. Out of 319 students only 6.26% showed positive for toxoplasmosis among those 2.50% were males and 3.76% were females. The positive result in RT - PCR analysis were distributed on the patterns of the anti - Toxoplasma antibodies by ELISA test , Any positive blood samples with IgM, IgG and both IgM and IgG respectively have not been found whereas 20 positive cases of no anti - Toxoplasma antibodies.Real - Time PCR test in student's blood has advantages in detection of recent or active toxoplasmosis

التغيرات الفصلية والموقعية لتراكيز بعض العناصر النزرة في الرواسب والمياه والنبات المائي الشمبلان Ceratophyllum demersum في نهر الفرات عند قضاء سوق الشيوخ جنوب العراق == Seasonal and Reginal changes in concentration of some trace elements in sediment, water, and aquatic plant(Ceratophyllum demersum) in the Euphrates River at Suq Alshuyoukh districtsouthern of Iraq.

Author name: نور كاظم خير الله
Supervisor name: باسم يوسف الخفاجي
General topic: Biology
Specific topic: Environment - Environmental pollution
Degree: Master
University: University of Thi-Qar - College Of Science - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: The current study was performed to get the knowledge of seasonal and Reginal changes in concentration of some trace elements(Zn, Pb , Ni and Co) in sediment, water and aquatic plant (Ceratophyllum demersum) in the Euphrates River at Suq _ Alshuyoukh district, south of Iraq . The study was include some of the chemical and physical properaties for water (air and water temperature,turbidity, electrical conductivity,salinity,dissolved oxygen , biochemical oxygen dimand, pH,nitrate,phosphat also total organic carbon and sediment texture). The samples were collected from the river on seasonaly from Autumn 2015 to summer 2016. There are three stations were selected for the study , the first station was near by the tourist Alfadhliyah bridge, afertile farmland on both sides of the river. The second station was in Suq_Alshuyokh , it's away from the first station about 15 km , it's a center of boats which used for fishing ,agriculture lands and homes. The third station was located in stream of ALkermashiya which lieson snuffa_marsh (apart of the Hammar marsh) ,It's away from the second station about 11 km and it's near by villages and fertile farmland. The results of study showed that air temperature was ranged between (15.8 - 49.5)° C and water temperature was ranged between (13.8 - 48.7 ) °C . The highest temperature recorded in the third location during Summer. Turbidity concentrations ranged between (5.7 - 15.6) NTU. The electrical conductivity are ranging between (3920 - 8336) Ms/cm , and salinity of water was low (Oligohaline) ranging between (2.50 - 5.33) ppt and recorded the highest concentrations in the third station . the highest value of pH was (8.9) in the secondstation and the lowest value was (7.3) in the first location while the Dissolved Oxygen concentrations ranged between (3.1 - 8.45) mg/L. The biological oxygen demand (BOD5) was ranged between (0.35 - 4.5) mg /L. As for concentration of nitrates was ranged from (0.02 - 6.33) mg/L. and phosphate ranged between (0.01 - 5.8) mg/L. the total organic carbon as percentage was ranged between (0.48 - 3.1) %.while sediment texture was mixed from sand ,silt,clay,the percentage for the mentiond compenents was,(50%,20% and 30%)in station 1,(40%,10%,50%)for station 2 and (10%,20% and 70%) for station3Atomic absorption spectrofotometer was used to estimate four trace elements (Zn,Pb, Co, and Ni) .The concentrations of the mentionel elements in water dissolved phase were (2.86,5.87,1.63,0.51) μg /L,while their concentrations in particulate phase were (12.48,28.21,17.74,1.08) μg /gm dry weight respectively. In sediment the concentrations averages oftrace elements were (142.73,127.70,176.82,29.24) μg /gm dry weight respectively.In aquatic plant (Ceratophylum demersum) the concentrations averages of trace elements were (123.32,117.54,157.54,27.90) μg /gm dry weight respectively . It concluded that the concentration of studied elements in sediment more than their concentration in water (dissolved and particulate) phase and more than their concentration in plant (Ceratophyllum demersum), also all concentrations of trace elements were in the international acceptable values.

دراسة مصلية ومناعية لداء المقوسات في مرضى القلب == Serological and Immunological Study of Toxoplasmosis in Heart Disease Patients

Author name: حيدر صباح عبد الحسين
Supervisor name: بسعاد عقرب العبودي | احمد حسن محمد
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University of Thi-Qar - College Of Science - Department Of Biology
Language: English
University location: Dhi Qar
First pages:
Abstract: Toxoplasmosis is the general term for infection and disease in human and animal caused by a parasite called Toxoplasma gondii, this disease is prevalent among different categories of people , Heart disease patients are one of them. Because of the dormancy status of T. gondii, there is ambiguity and variation in the immunity of patients. This study aimed to investigate the immune status of heart disease patients infected with toxoplasmosis, to detect the prospective relationship between heart disease patients and T. gondii, and investigate the relationship between toxoplasmosis and risk factors which included gender, age, blood group, contact with cats, and habitation.One hundred fifty samples of blood had been collected at period from August 2016 till November 2016 from patients with heart disease, they lying or visit Heart Medical Centre in Thi - Qar province. According to gender, the patients divide in to (81 males and 69 females) with age ranging 18 to 82 years. Also one hundred samples of blood had been collected from apparently healthy persons as negative control ,dividing according to gender in to ( 55 males and 45 females) with age ranging 19 to 56 years . All samples of both subjects had been tested by ELISA technique to detect anti - Toxoplasma Abs (IgG & IgM) . While the positive samples with toxoplasmosis were tested to detect the level of interleukins (IL - 10, IL - 12 and IL - 17).The overall of seropositivity of anti - Toxoplasma Abs that scored by ELISA in the current study was 44% in heart disease patients are higher than those in apparently healthy controls 19%, and there was a significant difference between them. There is a significant difference between male and female, in the seropositivity of anti - Toxoplasma Abs that scored in controls, in male was higher than those in female. Presence of antiSummary toxoplasmosis Abs in all types of heart disease patients with significant difference among them, and the highest percentage of anti - toxoplasmosis Abs in Mitral stenosis( MS) was 55.8% , and the lower value was 30.3% in Myocardiopathy.There are significant differences between the age groups and the percentage of anti - Toxoplasma Abs in heart disease patients with highest value was 63.2% at age group of 61 - 75 years, and the lowest one was 26.3% at age group of 15 - 30 years. While no significant differences between the age groups and toxoplasmosis in apparently healthy controls with highest value was 19.5% at age group of 31 - 45 years, and the lowest one was 8.6% at age group of 15 - 30 years.The results demonstrated that seroprevalence of toxoplasmosis was significantly associated with close contact with cats. The percentage of seroprevalence anti - Toxoplasma Abs in heart disease patients and controls inhabited rural area was 52.5% and 32% are higher than those inhabited urban area was 34.3% and 6% respectively. Some blood parameters were estimated of both subjects infected and non - infected with toxoplasmosis. There was high significant difference at p≤0.05 observed in mean ratio of eosinophil’s number between infected and not infected subjects with toxoplasmosis in both subjects. While there was no significant differences in mean ratio of total count of WBCs. Moreover, mean of lymphocytes number recorded significant differences betweeninfected and non - infected heart disease patients only. The blood group phenotypes of the infected heart disease patients and controls highlight the role in toxoplasmosis infection and the AB blood group characterized by the highest significant percentage of infection. The results of interleukins appeared high mean levels of IL - 12 82.85 pg/ml in heart disease patients infected with toxoplasmosis when compared with control 60.97 pg/ml, and there was significant difference between them. While there was no significant difference in IL - 10 and IL - 17 in both patients and controls. The results also showed decreased level of IL - 17 in patients with heart disease. The increased level of IL - 12 in patient with heart disease infected with toxoplasmosis indicate to the activity of cell mediated immunity in those patients and may be lead to provoke an inflammatory status in the heart valves and muscle then worse heart disease to be sever and need complicated treatments

دراسة وبائية فسلجية لطفيلي المقوسة الكونيدية Toxoplasma gondii لمرضى الغسيل الكلوي والسطان في محافظة ذي قار العراق == Epidemiological and Physiological Study of Toxoplasma gondii for Patients with Renal Dialysis and Cancer in Thi - Qar Province - Iraq

Author name: نور كاظم مطر الركابي
Supervisor name: بسعاد عقرب العبودي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University of Thi-Qar - College Of Science
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: The current study was conducted in Thi - Qar province to investigate of Toxoplasma gondii prevalence among renal dialysis and cancer patients by using Enzyme Linked Immunosorbent Assay test and also the study determined of some factors that may affected that may affected the prevalence of infection, such as age, sex, marital status, place of residence and the contact with cats and a number of chemical dosing that exposed to it cancer patient or the number of times the dialysis for renal dialysis patients.One hundred blood samples were collected from renal dialysis patient and one hundred samples others were collected from cancer patient from two hospital AL - Hussein Hospital and AL - Habboubi Hospital for the period from the beginning October 2015 to the end of the month of March 2016 and prepared a questionnaire form for each person to get their information, as well as the collection of 50 blood samples from (healthy) as control group . .The results of the current study showed that the prevalence of toxoplasmosis in Thi - - Qar province in both renal dialysis and cancer patients and control group were 28% and 36% and 24% respectively. recorded results of Enzyme Linked Immunosorbent Assay ELISA test results for a positive antibody IgG in both renal dialysis and cancer patients and control group 25% , 30% , 22% respectively. As for the IgM antibody study did not record any positive result in both categories of the study (dialysis and cancer) only control group was 2%. The result of IgG & IgM together in renal dialysis and cancer and control group 3%, 6%, 0% respectively. .The highest infection prevalence were detected in renal dialysis in the age group (40 - 49 years) amounted (26.67%) and less infection in the age group (60> years) with percentage of 23.08% . As for cancer patients, the highest infection prevalence in the age group (50 - 59 years) was 53.85% and were less infection in the age group (30 - 39 years) amountin 14.29%. .The current study recorded there is a significant differences at level of P <0.05 in infection prevalence accoriding to gender for renal dialysis patients as it was infection prevalence in males 35.18%, while in the females the infection prevalence was 19.56%. As for cancer patients the study recorded significant differences P <0.05 where the infection prevalence in females were highest compared to males where it was 38.59% and 32.55% respectivelyThe study did not show any significant differences P> 0.05 for the infection prevalence according to contact with cats in renal dialysis patients but the study found significant differences between married and unmarried where the infection prevalence among married 25.84%, while the unmarried 45.45% .The infection prevalence were in the City highest 29.27% compared to countryside which amounted to 22.22%, the study found that the increase in the number of times the dialysis increased incidence of infection prevalence it was 25.53% for patients who conducted the dialysis treatment more than six times and 16.66% for patients who have less than six times. In cancer patients, the study observed significant differences P <0.05 for the contact with cats and it was highest infection prevalence in the city amounted to 45.21% compared to countryside 11.11%. and recorded a high infection rates at married 37.90% either at unmarried did not show they have no infection by parasite. For factor chemical dosing show high infection rates in patients who have been exposed to more than one dose of a chemical 37.63% As for those who were not exposed to any chemical dose was ratios have 14.29% .and wasblood type B + higher infection in renal dialysis patients 40.74% either in the cancer patients were O+ highest amounted to 58.62 %The results of the blood Parameters which conducted for infected males by toxoplasmosis and non - infected had not noticed a change in the hemoglobin for both categories. As total count and differential white blood cells the study did not record significant differences in the renal dialysis patients except neutrophil blood cell was observed height in infected males than non - infected . as for Cancer group was a decrease in the total number and differential white blood cells except acidophilus did not record any change in its level. As the females the study results were that there was no significant difference for both renal dialysis and cancer patient except Lymphocyte found decline in infected than non - infected for cancer patients .showed the results of the current study for standards biochemical that there are no significant differences P <0.05 for renal dialysis and cancer patient in the case of urea, creatinine, the study do not found any effect of toxoplasmosis on the rates of both urea and creatinine. As albumin was observed to high at dialysis patients and low at cancer patients at infected with toxoplasmosis compared to non - infected either control group the study did not reported significant differences for urea and creatinine and albumin. either liver function the study did not find change in the level of liver enzymes (AST.ALT) in dialysis and cancer patients and control group (healthy) .The presnt study recorded there is no significant differences in lipid profile (HDL, LDL, VLDL, Cholestrol, Triglyceride) at infected and non - infected in renal dialysis goup either cancer patients was observed high (HDL, VLDL, Cholestrol, Triglyceride

دراسة تاثير بعض المستخلصات المعزولة من ,الطحلب الاخضر Cladophora sp. في بعض المعايير الفسلجية لاناث الجرذان المختبرية Rattus norvegicus السليمة والمستهدفة مختبريا ,بالسكري == Study effect of some isolated extracts of green alga Cladophora sp. in some physiological parameters of female rats Rattus norvegicus laboratory healthy and laboratory - targeted diabetes

Author name: زهرة شعيبث بزيع الماجد
Supervisor name: حسين يوسف خلف الركابي | خالد كاطع الفرطوسي
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: The current study involved isolations of green alga Cladophora glomerata from Euphrates River in the city of Nasiriyah and demonstrating of alcoholic extract and alkaloid effect with concentrations (2.5, 5 mg/kg ) in the body weight and some blood standards and iochemical for Female rats normal laboratory and experimentally in effected diabetic, attended the alcoholic and alkaloid extracts of the alga. It was aqualitative statements on extract to see content of chemical compound were tested for cytotoxicity alkaloid extract toward the red blood cells of human and at different concentration showed no toxicity to extract through out observation period . The record of alcohol and alkaloids extract of laboratory rats in the first and second experiments is the following : - I. Get a significant decrease (P<0.05) in the mean body weight to the animals two group and (third and fourth) groups compared with the first group in the first and second experiments, significant decrease of animals two groups (fifth and sixth) compared with (second group)in the second experiment.II. Get significant increase (p<0.05) in the sugar level in (the second group) compared with (the first group) and significant decrease (P<0.05) in the sugar level of animals (four, fifth and sixth) the groups compared with (the first and the second) groups respectively in the both experimental.III. Get a significant decrease (P<0.05) in the number of red blood cell in (the fourth and sixth) groups, compared with (the first and second) groups respectively and get decrease no significant (P>0.05) in the level hemoglobin (Hb), paked cell Volume (PCV) and platelets compared with (the first and second) groups respectively in the first group. IV. Get significant decrease in number red blood cell in (the fourth group) compared with (the first group) and get decrease no significant in platelets compared with (the first and second) groups, significant decrease in hemoglobin level in (fifth and sixth) groups compared with (second group), significant decrease of paked cell volume (PCV) in the (the three and sixth) groups compared with (the first and second) groups respectively in the second experiment. V. Results appeared in the first experiment get significant increase (P<0.05) of white blood cell in the (second,fifth and sixth) groups compered with the (the first and second) groups respectively, no significant increase (P>0.05) in the percentage of neutral blood cell compared with the (first and second) groups respectively, while his significant increase in the percentage of acidic blood cells in the (third and fourth) groups compared with the (first group), the increase significant (P<0.05) in the percentage of monocyte cells compared with the (first and second groups) respectively, also had a significant decrease (P<0.05)in the percentage of lymphocyte cells compared with the (first and second groups) respectively.VI. Result appeared in the second experiments a significant decrease in white blood cells of (fifth and sixth) groups compared with the (second group), get significant increase in percentage of neutral blood cell in(third and fourth) groups compared with the (first group) and get significant increase in the percentage of monocyte, acidic, monocyte cells in (the third and fourth) groups compared with (the first group) and (the fifth and the sixth) groups for acidic and lymphocytes cells compared with the (second group).VII. The results also reported a significant decrease (P<0.05) in the level malodialdyhde (MDA) for animals (sixth group) compared with the (second group) in the first experiment and significant decrease in the level malondialdyhde (MDA) for animals (fifth and sixth)groups compared with the (second group) in the second experiment and get no significant decrease of level ceruloplasmin and albumin compared with the first and second groups respectively in both two experiments .VIII. Results appeared a significant decrease (P<0.05) in the level cholesterol triglycerides(TG) and very low density lipoproteins compared with the ( first and second) groups respectively and get significant decrease (P<0.05) in low density lipoprotein in the (fourth group) compared with the (first group) and (fifth and sixth) groups compared with the (second group), and result appeared get no significant decrease in the level (ALT) enzyme compared with the (first and second) groups respectively, andno significant decrease in level (AST) enzyme in the (fourth group) compared with the (first group) and no significant increase in (sixth group) with the (second group), also get significant increase (P<0.05) in level (ALP) enzyme in (third group) compared with the (first group) in the first experiment and there are not occur significant differences in level liver enzymes (ALT,AST,ALP) in the second experiment . IX. Results appeared get significant increase (P<0.05) in level hormone (FSH) in (fourth group) compared with the (first group), significant increase in level hormone (LH) in (fifth and sixth) groups compared with the (second group), significant increase in level hormone Estrogen compared with the (first and second) groups respectively in the first experiment , and also there are no significant difference in level Femininity sexual hormones (FSH,LH,Estrogen) in the second experiment

التشوهات الخلقية في اجنة الفئران السويسرية البيضاء من امهات معاملة بجرع مختلفة من عقار Mus masculus الديكساميثازون == Developmental anomalies in swiss albino mice embryos Mus masculus from the mother treated with different doses of Dexamethasone

Author name: صدى غالب طاهر الموسوي
Supervisor name: علي نعيم سلمان
General topic: Biology
Specific topic: Zoology - Embryology
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences
Language: English
University location: Dhi Qar
First pages:
Abstract: The current study was conducted for the period from September 2015 until August 2016 in college of Education for Pure Sciences - University of Thi - Qar, this study aimed to fallow the embryonic lung development and evaluate histological changes in embryos's lung development when pregnant mice treated with different doses of Dexamethasone (Dex) and evaluate Alpha - fetoprotein (AFP) levels were evaluated as an indicator for congenital malformations. Sixty pregnant mice were randomly divided into four groups and each group contain 15 pregnant mice. The members of each group were given specific dose of Dex and at different time periods, while the control group injected with a solution of Normal Saline 0.9%.All animals received doses used by tail intravenous injection until the end of the time periods specified .Blood samples were taken for the purposes of biochemical parameters account as well as the collection of samples for histological study .The treatment of animals under the same conditions were determined with a dose based on body weight, according to what was stated in the pharmaceutical constitutions where's the therapeutic dosage was 0.002 mg \2mg (0.1mg.kg from body weight ). We used another two concentrations from drug ,one less than the therapeutic dosage (0.05mg \kg from body weight) and another more than the therapeutic dosage (0.2mg \kg from body weight ).The results have showed that was a negative effects on mice embryo's body weights and the lengths increase with increasing of number and doses concentrations. The using of different doses of Dex also showed various changes in general external morphological features and congenital malformation in embryos of treated mothers included : Death of embryos ,placenta damage ,neural Tube Defect ,Trunk Torsion ,Headhemorrhage, Brain hypertrophy ,Liver Hypertrophy ,Letter C Shape Embryos, swelling ,Convoluted Tail ,Short nostril and limbs, curved head, Wrinkled skin ,Crooked pave ,Short toes ,Seal legs appearance, Oedema ,absent fingers and eyelids ,Tail congestion and Bulging eyes. Histologically microscopic examination showed histological changes with varied severity and appearing between different sub experimental group compared with the control group.Rapid elongation in lung bud, advancing in lung development, the lung asflower appearance, Loosely lung mesenchyme ,necrosis in lung tissue ,mucus hypersecretion, elongation in epithelial air ways,epithelial Hyperplasia ,mesenchyme looks as butterfly in appearance in some parts ,thinning of alveolar walls ,thinning of the interstitialmesenchymal lung tissue ,elongation of developing alveolar septa ,free alveolar septa. Alfa - fetoprotein (AFP) levels showed different concentrations value in correlation with congenital malformations .when the (AFP) value was high there were neural tube defects and advancing in the lung development while they were low in normal lung development and in brain hypertrophy.

دراسة لنوعية المياه وتركيز الهيدروكربونات النفطية وبعض العناصر النزرة في المياه الجوفية للابار القريبة من مصافي الجنوب في منطقة الشعيبة - البصرة جنوب العراق == Study Water Quality and Concentration of Petroleum Hydrocarbons and Some Trace Elements in Groundwater of Wells Near South Refineries in Al - Shiaba region - Basrah, Southern Iraq

Author name: بثينة مهدي يونس
Supervisor name: باسم يوسف الخفاجي
General topic: Biology
Specific topic: Environment - Environmental pollution
Degree: Master
University: University of Thi-Qar - College Of Science
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: The present study was carried out to determine the concentration of total petroleum hydrocarbons in two phases dissolved and suspended in water, and Water Quality, in addition to three trace metals nickel Ni, lead Pb and cadmium Cd were determined in the groundwater, in eight wells. Six wells from W1 to W6 close to the source of pollution about half kilometer to three kilometer and two wells (w7 - w8) were far with in12.5Km from the mentioned source pollution area (well control).Wells were located on surrounding southern refineries (Al - hiaba ) - Zubair - Basra in southern Iraq .Water samples were collected seasonally from autumn 2015 to summer 2016.The present study including, the measurements of some environmental factors, water and air temperatures , salinity, electrical conductivity, turbidity, total dissolved soluble solids (TDS), pH , dissolved oxygen , total hardness and chloride ions .Results were ranged between ,( 19.5 - 48.8) ºC ( 25.0 - 29.3) ºC , (4.35 - 10.95) mg∕L, (5.01 - 11.45) mS ∕cm , (1.110 - 6.803) NTU , (3206 - 7328) mg∕L (7.05 - 8.72) , (1.35 - 12.25) mg ∕L ,(1275 - 4200) mg ∕L and ( 1560 - 5416) mg∕L respectively .Total petroleum hydrocarbons (TPHs) were determined using spectroflurometer . Results showed that concentrations of means TPHs as dissolved fraction in water were in all wells highest in spring 2016 than winter then autumn 2015, while less concentration recorded was during summer 2016.The highest mean concentration was 35.91 μg /L in well W1 in a spring 2016 while lowest value 0.73μg/L in well W7 in summer 2016 . Results of highest means concentrations of TPHs particular fraction in water were 137.39 μg /g dry weight in well W1 in spring 2016 and lower means concentrations of TPHs particular fraction 9.89 μg /g dry weight in well W8 summer 2016. Sixteen compounds of Polycyclic Aromatic Hydrocarbons(PAHs) were identified in water (dissolved and particulate fractions ) during spring 2016 using a liquid Chromatograph high performance (HPLC). The total concentrations of dissolved PAHs in water ranged between the highest concentration 7205 ng∕L in well W1, and lowest concentration 39.1 ng∕L in well W8 ,the highest concentration of Phenanthrene compound in well W1 was 3472 ng∕L and lower concentration for Benzo(a) Pyrene compound was 0.37 ng∕L in well W8,was the more dominate PAHs compound in dissolved phase recorded Benzo(b)Fluranthene .The total concentrations of PAHs particular fraction ranged between the highest concentration 9334 μg /g dry weight in well W1, while lowest concentration 370 μg /g dry weight in well W8.The more dominant PAHs compound as particular fraction Fluorene in spring season ,were compound Dibenzo(a,h)anthracene has a highest concentration 6689 ng∕g dry weight in well W3 and the to west concentration compound was Benzo(a)anthracene ng∕g in well W8. Results recorded highest concentration of lead 0.933 μg /L in well W1 in spring 2016 and lowest concentration 0.208 μg /L in well W7 in summer 2016. While means concentrations of nickel ranged between 0.445 μg /L in well W1 in winter 2016 and lowest concentration 0.055 μg /L in wellW8 in autumn 2015.Cadmium record its highest level 0.496 μg /L in well W3 in spring 2016 and lowest concentration 0.053 μg/L in well W8 in summer 2016.Results of this revealed that southern refineries have a major effects on ground water pollution in vicinity of oil fields by some hydrocarbons derived from those refineries

تقييم صلاحية مياه نهر الغراف - جنوب العراق للاستخدامات المختلفة باستخدام دليل نوعية المياه (النموذج الكندي) == Evaluation of the validity of The AL - Gharraf - River Water Southern of Iraq for various uses by using the Water Quality Index (Canadian Model)

Author name: مها مثنى حسن التميمي
Supervisor name: حسين يوسف خلف الركابي
General topic: Biology
Specific topic: Aquatic Environment
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: اجريت الدراسة الحالية على نهر الغراف في محافظة ذي قار(جنوب العراق) فصليا ابتداء من فصل الصيف 2014 ولغاية فصل الربيع 2015 باستعمال دليل نوعية المياه (النموذج الكندي ) في هذه الدراسة بوصفه اداة فعالة في تقييم نوعية مياه نهر الغراف ولاول مرة في منطقة الدراسة لتحديد صلاحيته لاغراض مختلفة منها الشرب ومعيشة الاحياء والري للوقوف على حال المياه ونوعيتها في هذا النهر . اختيرت اربع محطات على طول النهر لجمع عينات المياه تمثلت المحطة الاولى عند مدينة الحي في محافظة واسط قبل دخول النهر محافظة ذي قار واحداثياتها 32°08'29.4"N ; 46°02'38.3"E))، اما المحطة الثانية تقع بعد خروج النهر من قضاء الرفاعي وتبعد عن المحطة الاولى بمسافة 50 كم تقريبا واحداثياتها (31°41'21.6"N ; 46°06'53.9"E) ،تقع المحطة الثالثة بعد خروج النهر من قضاء الشطرة وتبعد عن المحطة الثانية بمسافة 40 كم تقريبا واحداثياتها (31°22'50.8"N; 46°10'51.0"E)، والمحطة الرابعة والاخيرة تقع عند مدينة الاصلاح وتبعد عن المحطة الثالثة 72كم تقريبا واحداثياتها( 31°09'55.0"N; 46°36'36.6"E). جمعت عينات المياه من محطات الدراسة فصليا ابتداء من فصل الصيف2014 ولغاية فصل الربيع 2015 .اختير26 متغيرا من المتغيرات ذات التاثير الاكبر في نوعية المياه وصلاحية استخدامها لتجهيز مياه الشرب ولاغراض معيشة الاحياء ولاغراض الري في ايجاد قيم الدليل وهي درجة حرارة الهواء والماء وسرعة الجريان والاس الهيدروجيني والاوكسجين المذاب والمتطلب الحيوي للاوكسجين والمتطلب الكيميائي للاوكسجين والتوصيلية الكهربائية والملوحة والعكارة والمواد الصلبة الذائبة الكلية والعسرة الكلية والكاربونات والبيكاربونات والقاعدية الكلية والكالسيوم والمغنيسيوم وايون الكلورايد وايون الصوديوم وايون البوتاسيوم ونسبة امتزاز الصوديوم والفوسفات الفعالة والنترات الفعالة والكبريتات والعناصر النزرة (الكادميوم والرصاص) وبكتيريا القولون البرازية . اوضحت نتائج الدراسة الحالية ان درجة حرارة الهواء تراوحت بين(10 - 44) ○م ،اما درجة حرارة الماء فكانت بين (7.3 - 28.5) ○م ، وسرعة الجريان بين(0.07 - 0.25)م/ ثا ،اما قيم الاس الهيدروجيني سجلت بين(5.92 - 8.5)،كما سجلت الدراسة الحالية تراكيز للاوكسجين الذائب بين(1.06 - 8.4)ملغم/لتر ،اما المتطلب الحيوي للاوكسجين فكان ضمن المدى(22. 1 - 3.24)ملغم/لتر، بينما قيم المتطلب الكيميائي للاوكسجين بين (6.2 - 35.4) ملغم/لتر، في حين تراوحت قيم التوصيلية الكهربائية بين(674 - 1622) مايكروسمنز/سم، كما تراوح تركيز الملوحة بين (0.43 - 1.04) غم/لتر والعكارة بينNTU 6.7 - 3.11))،كما سجل تركيز المواد الذائبة الكلية بين(336 - 902) ملغم/لتر، والعسرة الكلية بين (367 - 833) ملغم CaCO3/لتر وكانت قيم الكاربونات غير محسوسة والبيكاربونات بين و(294 - 605) ملغم CaCO3/لتر، كما سجلت الدراسة الحالية تراكيز القاعدية الكلية بين(152 - 301) ملغم CaCO3/لتر، وكانت قيم الكالسيوم بين (52.3 - 197) ملغم/لتر والمغنسيوم بين (35.6 - 134)ملغم/لتر، والكلورايد بين (118 - 312)ملغم/لتر، كما سجلت مديات ايونات الصوديوم بين (93 - 201)ملغم/لتر والبوتاسيوم(3.2 - 13.37)ملغم/لتر ،اما نسبة امتزاز الصوديوم فكانت بين (0.63 - 3.98)ملي مكافئ/لتر،واما الفوسفات فيقع بين (0.03 - 0.44)ملغم/لتر.، بينما قيم النترات تقع بين (1.35 - 4.1)ملغم/لتر، في حين تروحت الكبريتات بين (202 - 399)ملغم/لتر. كما اظهرت الدراسة الحالية ان قيم العناصر النزرة كانت في الطور العالق ، اعلى من الطور الذائب اذ سجل عنصر الرصاص في الطور الذائب ما بين (1.72 - 47.81)مايكغم/لتر بينما في الطور العالق بين(5.78 - 135.1) مايكغم /غم وزن جاف. اما قيم عنصر الكادميوم في الطور الذائب بين (1.46 - 10.08)مايكغم وفي الطور العالق بين(2.16 - 110.72) مايكغم /غم وزن جاف. كما سجلت مياه المحطات المدروسة اعدادا لبكتريا القولون البرازية بين(50 - 333)خلية/100مل تراوحت نتائج الدراسة الحالية لقيم دليل نوعية المياه لتجهيز مياه الشرب(PWSI) بين (19.87 - 26.98) لكافة المحطات طوال مدة الدراسة وهي بذلك تصنف ضمن الفئة الخامسة 44 - 0)) الفقيرة (Poor) على مقياس الدليل, اذ سجلت اعلى قيمة للدليل(26.98) (Poor) في المحطة الثانية خلال فصل الشتاء وادنى قيمة للدليل(19.87) (poor) في المحطة الرابعة لنفس الفصل. اما قيم دليل نوعية المياه لاغراض معيشة الاحياء (WQIAL) فكانت بين(4.37 - 12.06) وهي بذلك تصنف ضمن الفئة الخامسة ايضا 44 - 0))الفقيرة(Poor) لجميع المحطات طوال مدة الدراسة ,اذ سجلت اعلى قيمة للدليل (12.6 ) (poor) في المحطة الثالثة خلال فصل الشتاء وادنى قيمة (4.37) (poor) في المحطة الرابعة خلال فصل الصيف. في حين وجدت قيم دليل نوعية مياه الري (IWQI) ما بين (16.11 - 43.32) وهي بذلك تصنف ضمن الفئة الخامسة 44 - 0))(Poor) على مقياس الدليل, لجميع المحطات طوال مدة الدراسة ,اذ سجلت اعلى القيم للدليل في المحطة الاولى ( 43.32) (poor) خلال فصل الشتاء وادنى قيمة(16.11)(poor) في المحطة الرابعة خلال فصل الصيف. اظهرت نتائج تحليل المكونات الاساسيPrincipal Component Analysis (PCA) ان الكادميوم والبوتاسيوم والنترات والفوسفات والكلورايد والمتطلب الكيميائي للاوكسجين وبكتريا القولون البرازية والمغنيسيوم اهم المتغيرات المسؤولة عن التغاير في نوعية مياه الشرب وبالتالي اكثرها تحديدا لمدى صلاحية نهر الغراف لتجهيز مياه الشرب, تلاها الصوديوم والعسرة الكلية والكبريتات والعكارة والاوكسجين المذاب والرصاص والقاعدية الكلية والتوصيلية الكهربائية والمواد الصلبة الكلية والكالسيوم والاس الهيدروجيني والمتطلب الحيوي للاوكسجين .في حين اهم المتغيرات المسؤولة عن التغاير في نوعية المياه لاغراض معيشة الاحياء ،درجة الحرارة والمتطلب الكيميائي للاوكسجين وبكتريا القولون البرازية والبوتاسيوم والفوسفات والكلورايد والكادميوم تلتها المتطلب الحيوي للاوكسجين والعكارة والاس الهيدروجيني والرصاص والمواد الصلبة الذائبة الكلية والاوكسجين المذاب والعسرة الكلية . في حين كان الاس الهيدروجيني والبيكاربونات والرصاص والتوصيلية الكهربائية هي المتغيرات الاكثر مسؤولية في التاثير على نوعية مياه الري ،تلاها الكادميوم ونسبة امتزاز الصوديوم والكلورايد. | The current study was conducted on Al - Gharraf River in Thi - Qar governorate(southern of Iraq) for the period from Summer 2014 to Spring 2015 using water quality index(Canadian model) in this study as an effective tool in the assessment of Gharraf River water for the first time in the study area to determine suitability for drinking, irrigation and aquatic organisms life. Four stations were determined along the river to collect samples AL - Hay city to the completion in the marshes .The first station was located in the district city and away from the second station of 50 kilometers (32 ° 08'29.4 "N; 46 ° 02'38.3" E), The second station was located after the departure of the river to spend AL - Rifai and its coordinates (31 ° 41'21.6 "N; 46 ° 06'53.9" E), The third station was located after the departure of the river AL - Shatra and away from the second station of approximately 40 km (31 ° 22'50.8 "N; 46 ° 10'51.0" E) The fourth station was located at the AL - Esllah city and away from the third station about 72km (31 ° 09'55.0 "N; 46 ° 36'36.6" E). Water samples were collected seasonally from a quarterly of study stations (and by four samples in the chapter) during every two months, starting from summer 2014 until spring 2015. twenty even variables were compiled the greatest impact on the water quality of the river and the validity of the Garraf which have using for the processing of drinking ,for the purposes of living requirements and irrigation purposes in the directory to find the WQI. These variables were air temperature, water temperature, the water current velocity, pH ,dissolved oxygen ,biological oxygen demand, chemical oxygen demand and electrical conductivity, salinity, turbidity ,total dissolved solids ,total hardness, carbonate ,bicarbonates ,total alkalinity, calcium ,magnesium, ion chloride ,sodium ion and potassium ion, reactive phosphate, reactive nitrate, sulfate sodium adsorption ratio ,trace elements (cadmium and lead) and fecal coliform bacteria. The current results showed that the air temperature ranged between (10 - 44) Cº, while the temperature of the water ranged between (7 - 28) Cº, the water current velocity ranged from (0.25 - 0.7) m / sec, pH between (5.92 - 8.5), The current study also recorded the concentrations of dissolved oxygen between (1.06 - 8.4 mg / L). The concentration of biological oxygen demand within the range (1.22 - 3.24) mg / L . The chemical oxygen demand of between (6.2 - 35.4)mg / L, Electrical conductivity values ranged between (674 - 1622) μs / m, .Salinity concentration ranged between (0.43 - 1.04) mg / L .Turbidity of water ranged from (3.11 - 6.7)NTU. Total dissolved solids ranged between (336 - 902) mg / L. Total hardness ranged between (367 - 833) mg CaCO3 /L. Carbonate values was non - palpable and bicarbonate and ranged between (294 - 605) mg CaCO3 /L. The total alkalinity concentration between (152 - 301) mg CaCO3 /L, Calcium ranged between (52.3 - 197 )mg / L. Magnesium ranged between (35.6 - 134) mg / L. Chloride ranged between (118 - 312) mg / L. Sodium ions were recorded between (93 - 201) mg / L. potassium (3.2 - 13.37) mg / L. The proportion of sodium adsorption ranged between (0.63 - 3.98) mequ/L. Phosphate ranged between (0.03 - 0.44) mg / L. Nitrates ranged between (1.35 - 4.1) mg / L. Sulfates ranged between (202 - 399) mg / L. The finding showed that the values of trace elements in the airborne phase was higher than the dissolved phase range the lead element of the dissolved phase between the values (1.72 - 47. 81) μg/L, while in the suspended phase ranged between(5.78 - 135.1) μg /gm dry weight. In the case cadmium of values of soluble part in the water range was (1.46 - 10.08) μg/L, either in the suspended phase of the water was (2.16 - 110.72) μg /gm dry weight. Studied water stations also recorded Numbers of fecal coliform ranged from (50 - 333) cells / 100 ml. Was also recorded in the studied stations . The values of potable water supply index (PWQI) ranged between (19.87 - 26.98) for all stations accordingly it is categorized as fifth grade(0 - 44) (Poor). The highest value was (26.98) (Poor) in the second stations through the winter and the lowest value of the index (19.87) (poor) in the fourth station of the same season. Water quality index for aquatic organisms life (WQIAL) ranged between (4.37 - 12.06) for all stations there for it is evidently categorized as fifth grade(0 - 44) (Poor) , As it recorded the highest value of index 12.6 (poor) in the third stations during the winter and the lowest value of index was( 4.37) (poor) in the fourth stations during the summer. The values for irrigation water quality index (IWQI)ranged between (16.11 - 43.32) for all stations accordingly it is categorized as fifth grade(0 - 44) (Poor) , As it the highest values of the index in the first station 43.32 (poor) during the winter and the lowest value of index was (16.11) (poor) in the fourth station during the summer. The results of Principal Component Analysis (PCA) showed that cadmium, potassium, reactive nitrates, phosphates, chloride ,chemical oxygen demand ,fecal coliform and magnesium were the most effective variables on the changes in water quality index for drinking water , followed by sodium, sulfates, total hardness ,turbidity ,dissolved oxygen, lead , total alkalinity ,electric conductivity ,total solids, calcium, pH and biological oxygen demand. While the most important variables responsible for the variation in water quality for quality organisms life,were water temperature ,chemical oxygen demand ,fecal coliform, potassium, phosphate, chloride , cadmium, followed by biological oxygen demand ,turbidity, pH, lead, total solids, dissolved oxygen and total hardness. While the pH ,bicarbonates, lead and electrical conductivity were the most influents effective on the determination of irrigation water quality, followed by cadmium, sodium adsorption ratio and chlorine .

التغيرات النسجية لتاثير اشعة الهاتف النقال في بعض اعضاء طائر السمان الياباني (Coturnix coturnix japonica) == The histological changes to the effects of mobile phone radiation in some organs of ( Coturnix coturnix japonica )

Author name: احسان فرج عبد الصاحب الحسناوي
Supervisor name: ستار عبود فارس
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: تضمنت الدراسة الحالية استـعمال (48) طـيـرا مـن ذكـور واناث طائر الســمان الـيابانــي Coturnix coturnix japonica، التي كانت بعمر ( 60 الى 70 ) يوما , تم الحصول على الطيور من الاسواق المحلية لمدينة سوق الشيوخ / محافظة ذي قار / العراق ، وبعد فحصها من قبل الاطباء البيطريين والتاكد من كونها سليمة وضعت الطيور معا لمدة (30 يوما) لغرض التكيف ومن ثم قسمت الطيور الى اربع مجاميع ووضعت في اقفاص خشبية بابعاد (50 × 50 سم3 ) وبواقع (12 طيرا) في كل مجموعة (6 ذكور و6 اناث) , وضعت الاقفاص في ظروف مناسبة من حيث التهوية والاضاءة ودرجة الحرارة التي بلغت من (23 - 25 مº) ، عرضت المجموعة الاولى لاشعاع الهاتف المحمول لمدة (7 ايام) , وعرضت المجموعة الثانية لمدة (30 يوم) , والمجموعة الثالثة لمدة (60 يوم) , اما المجموعة الرابعة فهي مجموعة السيطرة , وبعد انتهاء فترة التعرض شرحت الـطـيـور واسـتخـرجـت الاعـضاء الـمـراد دراسـتـها (الـخـصى ، الـمـبايـض ، الـكـبـد ، الـكـلى) . اظهرت نتائج الدراسة الحالية حصول انخفاض معنوي ( P≤0.05) في معدل اقطار النبيبات المنوية وكذلك حصول انخفاض معنوي في معدل سمك طبقة الخلايا الجرثومية في الخصى وكذلك حصول انخفاض معنوي في معدل وزن الخصى في ذكور المجاميع المعرضة لاشعاع الهاتف المحمول مقارنة مع مجموعة السيطرة كما اظهرت نتائج الدراسة الحالية حصول تغييرات نسجية في خصى ذكور المجاميع المعرضة لاشعاع الهاتف النقال تمثلت هذه التغيرات بحصول نقصان في اعداد الحيوانات المنوية وقلة في اعداد الخلايا المكونة للنطف ونقص في كثافة النسيج الضام الذي يفصل بين النبيبات المنوية وكذلك تحلل degeneration في النسيج الضام بين النبيبات المنوية بالاضافة الى تحلل في جدار النبيب المنوي وكذلك حصول تنخر في الظهارة النبيبية وحصول نزف دموي بين النبيبات المنوية ، واظهرت نتائج الدراسة الحالية للمبيض حصول انخفاض معنوي في معدل وزن المبيض في المجاميع المعرضة لاشعاع الهاتف النقال مقارنة مع مجموعة السيطرة كما اظهرت الدراسة للمبيض في اناث المجاميع المعرضة لاشعاع الهاتف النقال لمدة (30 و60 يوم) حصول تحلل في جدار المبيض وقلة في اعداد الحويصلات المبيضية وكذلك احتقان في الاوعية الدموية ونزف دموي بالاضافة الى ارتشاح الخلايا الالتهابية , كما اظهرت نتائج الدراسة للكبد في المجاميع المعرضة لاشعاع الهاتف النقال حصول توسع expansion في الجيبانيات الكبدية واحتقان congestion وتوسع في الوريد البابي المركزي وكذلك حصول تفجي vaculation في سايتوبلازم الخلايا الكبدية بالاضافة الى ارتشاح الخلايا الالتهابية وحصول نزف دموي ، كما اظهرت نتائج الدراسة الحالية ان تعرض طيور السمان الياباني لاشعاع الهاتف النقال يؤدي الى حصول انخفاض معنوي في معدل قطر الكبيبة الكلوية وارتفاع معنوي في معدل الحيز بين الكبيبة ومحفظة بومان وارتفاع معنوي في معدل قطر النبيب الكلوي عند المقارنة مع مجموعة السيطرة كما اظهرت نتائج الدراسة الحالية للكلى في المجاميع المعرضة لاشعاع الهاتف النقال حصول ارتشاح infiltration للخلايا الالتهابية ونزف دموي hemorrhage وانكماش shrinkage في الكبيبات الكلوية بالاضافة الى حصول تحلل degeneration في النبيبات الكلوية . | The current study includes use (48) birds from both sexes (males , females) from Coturnix coturnix japonica . These birds were brought from the local market of Suq Al - shuyukh city / Thi - Qar province / Iraq , and then checked by veterinarians and make sure they are healthy we put them together for (30) days for adaptation and then divided the birds into four groups and put in wooden cages with dimensions 50 × 50 cm3 for 12 birds in each group (6 males and 6 females) , the cages put at organization and controlled whence ventilation , lighting and temperature ranged between (23 - 25 Cº) , the first group was exposed of mobile phone radiation for (7) days , and exposed a second group for (30) days , and the third group for (60) days , while the fourth group a control group , and after the end exposure period vivisection the birds and we extracted the organs we want study (testes , ovaries , liver , kidneys). The results of the current study showed a reduction in the rate of the seminiferous tubule diameters as well as a reduction in the rate of germ cells layer thickness in testes males mobile phone radiation - exposed aggregates compared with control group , the results of the current study also showed histological changes in testis males groups mobile phone radiation - exposed these changes were decrease in sperm counts and lack of number of cells of sperm and a decrease in density connective tissue which separates between seminiferous tubules and degenerating in connective tissue between seminiferous tubules addition to smashed in the wall of seminiferous tubule as well as epithelium necrosis and hemorrhage between seminiferous tubules , also showed the results of the current study of the ovary in females mobile phone radiation - exposed groups for (30 and 60) days smashed into the wall of the ovary and lack in numbers ovarian follicles and vascular congestion and hemorrhage in addition to infiltration of inflammatory cells , also showed the results of the current study of the liver in groups mobile phone radiation - exposed expansion in sinusoids, congestion, expansion in central vein and vaculation in hepatocytes in addition to infiltration of inflammatory cells and hemorrhage , as the results of the current study showed that exposure to radiation Japanese quail mobile phone leads to a reduction in renal glomerulus diameter rate and high rate of spread between the glomerulus and Bowman's capsule and high renal tubule diameter rate when compared to the control group and the results of the current study showed that the kidneys in mobile phone radiation - exposed aggregates infiltration of inflammatory cells, and hemorrhage shrinkage in renal glomerulus in addition to getting degenerate renal tubules .

عزل وتشخيص المضاد الحيوي المنتج بواسطة عزلة Penicillium chrysogenum من البيئات المختلفة في ترب مدينة الناصرية == Isolation and Identification of antibiotic produced by Penicillium chrysogenum isolated from different environments in Nasseriya city soi

Author name: ميسم كريم حسن
Supervisor name: سعد سلمان هميم | محمد حسين مشهد
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University of Thi-Qar - College Of Science - Department Of Biology
Language: English
University location: Dhi Qar
First pages:
Abstract: The present study includes the isolation and identification of some mycoflora from 40 soil samples in six places ( Remnants of fat - born, parks, edges of the river, animal wastes, sewage and rubbish) during October 2015 - January 2016 in Nasseriya City, Iraq. According to different environmental factors, the isolated genera were Aspergillus, Penicillium, Mucor, Rhizopus, Cladosporium, Sepedonium chrysospermum, Alternaria chlamydospora, Bipolaris, Chrysosporium, Candida albicans, Rhododendron flavum, Humicola, Geotrichum candidum, Fusarium and Acremonium. Three isolation methods were used. Dilution method, direct plate method, alcohol and heat treatment technique using the cultural media viz. Potato Dextrose Agar ( PDA), Sabouraud Dextrose Agar (SDA) and Potato Carrots Agar (PCA). Aspergillus represented the highest fungal isolates which represent 62 (37.12%). followed by Penicillium with 47 (28.14%), Mucor 22 (13.16%), Rhizopus 15 (8.98%), Cladosporium 6 (3.59%), Sepedonium chrysospermum and Alternaria chlamydospora, 3 (1.80%), Biopolaris, Chrysosporium and Candida albicans with 2 (1.20%), and finally Rhododendron flavum, Humicola, Acremonium, Fusarium and Geotrichum candidum recorded the lowest fungal isolation with one isolate for each (0.60%). The study was aimed to isolation of Penicillium from soil and assay its antibacterial activity. The results showed that dilution method gave a best fungal growth in comparison with direct plate method and alcohol and heat treatment technique in 25 ºC and pH = 6. PDA appeared as an optimum medium for isolation in comparison with other media such as SDA and PCA. The preliminary results showed that Penicillium sp. exerted antibacterial activity against Gram positive and Gram negative bacteria; Therefore, this fungus was used for production of antibiotic on Potato Dextrose Broth (PDB) medium. The optimum conditions were obtained at pH = 6, incubation temperature of 25 ºC and shaking rate of 180 rpm for 7 days to fermentation. Ethyl acetate was a good organic solvent to extraction of antibiotic which produced (3g/L) as white to creamy crystals. The characterization of the antibiotic product after extraction and purified by chemical methods included Thin Layer Chromatography (TLC) test, Nuclear Magnetic Resonance (NMR) Spectra and Mass Spectra. The Minimum Inhibitory Concentration (MIC) for clinical bacterial species was 1 - 10mg/ml.The PCR method used in this study utilized Internal transcribed spacer (ITS1 - 4) as a primer for identification of isolates. Two PCR products of the targeted gene of Penicillium chrysogenum isolates which locally named as (Penicillium chrysogenum F1 and Penicillium chrysogenum F2) were selected and subjected to partial DNA sequencing for the ITS gene to follow up the possible molecular relationship between these local isolates and what recorded globally in Genbank.

Predictability of the Expression of ER, PR Receptors, HER2 Marker by the Coloration with of IV Collagen Expression and AgNORs in Breast Carcinoma among Iraqi Women in Thi_Qar Providence : Immunohistochemical Study == Predictability of the Expression of ER

Author name: نجم الازيرجاوي
Supervisor name: هيفاء عبد علي الشمري
General topic: Biology
Specific topic: Life Science
Degree: Master
University: University of Thi-Qar - College Of Science
Language: English
University location: Dhi Qar
First pages:
Abstract: This study is adescriptive exploratory study in Thi - Qar providence for some breast cancer patients . This study aims to assess the relationship between gene expression for each type four of the collagen (IVCollagen) and gene expression positive for both estrogen receptor ER - Eestrogen - receptor and progesterone PR - Progestron receptor and fairs tumor HER2 (Human epidermal growth factor receptor) By using chemistry of dyes immunological technique and the nuclear regulatory regions Argyrophilic nucleolar organiser region (AgNOR(. The present study addresses the identification of the type IV collagen content in the basement membrane of epithelial tissue of the breast cancer patients in Thi qar and its relationship to nuclear organization region and maintain its relationship with hormonal receptors and progesterone genetic factor and the extent of their association with the occurrence of breast cancer.Fifty tissue samples are collected from Hussein Teaching Hospital inThi Qar and some private laboratories in the providence each case where data included that the most important factor is age, where he found that the highest percentage of injuries, age (55 - 46 years) increased by 38%. And mixed samples, either partial removal of the tumor or the eradication of whole breast. After collecting samples saved each formalin concentration of 10% for 24 hours at a minimum and then buried with parafin wax and completed the work by histological methods own work.The use of special select number of collagen type IV and determine the type of hormonal receptor positive or negative and also used several positive pathological tissues of the tumor to determine the human epidermal growth factor receptor by using silver nitrate to study the nuclear organization regions. Results of the current study showed that there is a positive relationship between collagen gene expression of type IV and between fairs oncogene(HER2 human epidermal growth factor receptor), as observed the presence of collagen type IV in tissue sections of tumors with HER2 negative gene expression of genetic factor, and with the positive expression for each Estrogen receptor positive ( estrogen positive ) and progesterone receptor positive( Progstrogen positive).Results of this study also showesthe presence of collagen or lack in histological sections of the tumors with positive gene expression of the HER2 oncogene fairs as well as the non - existence of collagen in the sections taken from tumors with negative expression of estrogen and progesterone receptors. The results of the current study also showed that there is a clear positive relationship between nuclear organization regions and between Argyrophilic nucleolar organiser region (AgNOR) Fairs oncogene HER2, as it was noted the presence of many of the nuclear regions of the dye - derived tissue sections of tumors with positive gene expression of the HER2 oncogene fairs, while nuclear chromosomal regions did not appear in the sections derived from tumors with negative expression of estrogen receptor and progesterone hormone. The results of the current study also showed that there is a lack of positive gene expression of collagen type IV, the presence of a number of chromosome regions nucleuse (Argyrophilic nucleolar organiser region (AgNOR)).

تاثير داء السكري وعقار البنتوستام على تطور الاصابة بداء اللشمانيا الاحشائية في ذكور الفئران المختبرية == The Impact of diabetes and Pentostam on development the infected Leishmania donovani parasite in male laboratory mice

Author name: افراح علي عبد الامير العزاوي
Supervisor name: فاضل عباس منشد العبادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University of Thi-Qar - College Of Science - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: This study included evaluation of the role of diabetes and Sodium Stibogluconate drug (Pentostam) against infection leishmaniasis parasite in laboratory animals experimentally infected with the parasite, Where we used in this study male white laboratory rats of the strain Mus musculus Balb /c. Aggregates were divided randomly into eight major groups of eight mice, The first group were injected with saline solution at a dosage of 0.2 ml, the group was considered as a negative control, The second group were injected with parasite (1.2 × 10 6 parasites / 0.2 ml) at a dosage of 0.2 ml, The third group was injected with the parasite and alloxan at a dosage of 0.2 ml, while the fourth group were injected only with alloxan, While the fifth group were injected with a Pentostam drug at a dosage of 20 mg/kg, as well as the sixth group were injected with leishmania parasite at a dosage of 0.2 ml and Pentostam drug at a dosage of 20 mg/kg while the seventh group were injected with the parasite and alloxan at a dosage of 0.2 ml and Pentostam drug at a dosage of 20 mg/kg while the eighth group injected with alloxan at a dosage of 0.2 ml and Sodium Stibogluconate drug at a dosage of 20 mg/kg, Where it was dissected first four groups a period of 45 days while the other groups were dissected after 75 days with recorded weights of the body before and after the experiment. Also studied the histological pathological changes and taking weights for each of the liver and spleen, blood samples were taken for the purpose of knowing the effect of which occurred in the blood parameters and the level of glucose in the blood. The results of the statistical analysis of the current study showed in the second set significantly higher (P≤0.05) total weight of the body and the weight of the liver spleen, White blood cells lymph and acidophilus, while there was a significant decrease in the total number of white blood cells, neutrophils and red blood cells there was no significant difference (P≤0.05) inthe blood glucose concentration compared with the first group. The fourth group showed a significant decrease (P≤0.05) in total body weight and red blood corpuscles with (P≤0.05) blood glucose level and neutrophil cells, the groups treated with the drug showed the sixth and seventh group height body weight and white blood cells and lymphocytes and a significant decrease (P≤0.05) red blood cells, neutrophils and acidity. There was no significant difference (P≤0.05) in the concentration of glucose in seventh group. The eighth group showed a significant decrease (P≤0.05) in the total number of white and red blood cells and significant increase (P≤0.05) in lymphocytes and acid, while there was no significant difference (P≤0.05) concentration of blood glucose. The fifth group showed the treatment of the drug loss in the radiology of hepatic cells and degeneration of clear and congestion in the central vein with the infiltration of inflammatory cells within the hepatic tissue, and showed a clear exhaustion in the white pulp and the proliferation of red pulp.we observed in the histological sections of the present study in the sixth group infected with parasite and treated with pentostam, a significant improvement of the liver tissue. The spleen showed a simple propagation of the white heart. The seventh group is experimentally infected with leishmania and the alloxan The development of diabetes and the treatment of pentostam is spread with Kupffer cells and expansion in the blood sinusoids with changes in the spleen. White and the group of nematodes around it with breeding in the red pulp as in the fifth group.

دراسة انتشار ذبابتي الرمل paptasi Phlebotomus و(Diptera : psychodidae) p.sergenti ودورهما في نقل مرض اللشمانيا في محافظة ميسان == Study the Prevalence of sand fly Phlebotomus p.sergenti (Diptera : psychodidae) paptasi and and its role in the spread of Leishmaniasis in Maysan province

Author name: عبد الحسين شلويط زغير
Supervisor name: صادق صاحب علي | بسعاد عقرب العبودي
General topic: Biology
Specific topic: Zoology
Degree: Master
University: University of Thi-Qar - College Of Science
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: during field studies have been done in order to know the spread of sand flies in Maysan province during the beginning of October /2015 until the end of September 2016 through the collecting of samples from five areas that geographically different which included the areas of AL - majer AL - kabber, AL - uzair, AL - musharah , kumait and Mugharbah in the middle of the center of province .1263 insect have been collected by two types of traps light insect traps and manual insect traps. These insects included two species : papatasi and sergenti which belong to the same genus ( phlebotomus ) the number of the first species insects was more than of the second species. The number of the first species reached 85.90% while the number of the second species was 14.04% . The inset had been classified in transmitted diseases center in BaghdadThe insects have been found in the whole areas of the studying except the species sergenti which has been not found in the area of AL - majer AL - kabber . There are two peak spread times of these insects : The first is through out October and the second is the through out may . Concerning the relationship between the spread of these insects and the occurrence of Leishmaniasis positivewhere the infestation by this disease began on September November and December and the peak spread is recorded on January on the country of the inset occurrence where it begins at spring and the beginning of autumn . The spread of these insects was in flounced by the environmental factors such as temperatures and humidity . The flagellated promastigotes of leishmania parasite were not found during the anatomy of a lot of insects.Four chemical insecticides were used to control these insects where the effect of the insecticides (snake) was the strongest for killing these insects.515 infection of Leishamaniasis has been recorded in Maysan province at proportion of 506 cutaneous infestation and 9 visceral infection respectively .The rate of skin infestation was high in age groups between (1 - 10 ) year in comparison with another age groups.Concerning the types of ulcer ,the dry ulcer were dominant and the number of dry ulcers was 873 while wet ulcers were 100 concerning the diameter of the ulcer the higher infections occurred at diameter of 1 Cm which reached 873 infection . The number of patients infected by one ulcer was the highest which reached 301 infestation.The number of dry infestation in the cities was higher than in rural areas on the contrary of wet infestation . concerning the distribution of skin infestation on the body areas the higher ratio of infestation occurred in higher and lower extremities which reached 344 at proportion of 68.57%.The infestation of skin Leishmaniasis has been concentrated in the center of province which reached 334 at proportion of 66.07%.Concerning the months of the year , the majority of skin leishmaniasis was on January which reached 137 at ratio of 27.66%most of visceral leishmaniasis occurred in the patients of blood group type O proportion of 33.33% .The infection of visceral Leishmaniasis in males was higher than of females and the highest than and the highest infection of visceral Leishmaniasis was in age groups between (1 - 3) years and also the number of visceral Leishmaniasis infestation was the highest on January . Kumait areas is characterized by the presence of the highest visceral Leishmaniasis infestation.

دراسة تجريبية وجزيئية للبكتريا المصاحبة لحالات الحروق في محافظة ميسان == Experimental and Molecular Study of the Bacteria associated with burns in Maysan Governorate

Author name: حسين علي دنانه البهادلي
Supervisor name: رحمن لعيبي جلاب | حيدر خميس شنان
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: Burn injuries are considered as health problems that may sometimes lead to rapid death. Bacteria are considered as the most important causes of microbial infection associated with burns. In present study, 75 clinical samples were collection from patients with burns for the period from October 2015 to June 2016 from the lobby burns in Sadr Teaching Hospital in the Maysan Governorate, these burn swabs revealed 56 bacterial isolates, 41 (73.21%) were Gram - negative bacterial, distributed of (35.71%) Pseudomonas aeruginosa, (28.57%) Klebsella Pneuomonia, (3.57%) Escherichia coli ,Enterobacter (5.35%). On the other hand 6(10.71%)were Gram - positive bacterial. out of 75 burned swabs, 84% revealed a single pathogens and 16% shown a mixed bacteria. On the other hand, Antimicrobial susceptibility for the bacterial burn isolates revealed that P. aeruginosa was resistant to routine tested antibiotics, like Amoxicillin, Gentamycin and Tobramycin in 100%, While was resistance to Ciprofloxacin 85%, while most P. aeruginosa was sensitive to Ceftizmiden in 50%. Results also showed that Staph. aureus was resistant to Gentamycin, Tobramycin, and Levofloxacin in 50% respectively.Depending on the rate of injury burns microbial and resistance to antibiotics used, p.aeruginosa and Staph.aureus isolated from the burnswere chosen and study their role in the events of infection, experimental burns, and for this purpose has been the use Albino mouse male as a model for a pilot study for pathogenesis and progress associated with the infection of the disease bacterial burns. (33) male mice were divided into tow groups, the first group(24) mice were burned (deep dermal second degree) and infection by 0.2X108 cfu kg of elected isolates, the route of infection was subcutaneously and scratching within burned area. In the second group six were infected without burn, which was considered as positive control, while in the thirdgroup three mice were burned and injected by 0.2ml of normal saline and consider as negative control .the first group was subdivided into two subgroups (12) mices for each burn dominant pathogens, then divided into subgroups each of six mices and following up (3,7, days intervals post infection process). P.aeruginosa burned mice group, shown skin infection more progressive and more severity in comporning with group infected by Staph. aureus, In addition, they were significant increases in the body temperature of all infection burned mice group(p.value)compared with those pre - infection process. Reisolation of bacteria from infection mice organs revealed, that high percentages in experimental p.aeruginosa burned model comparison with Staph. aureus.Diagnosed bacterial isolates by API 20and Vitek compact system, in addition to molecular diagnostics using PCR technique for the detection of 16S rRNA gene, All isolates showed a positive result of this gene. The amplified 16S rRNA gene seguence was compared with the seguence in NCBI seguence database.the bacterial strain identified as P.aeruginosa EPSI. This study reflects the usefulness of sequence analysis of the 16S ribosomal RNA gene in identifying bacteria and determining bacterial diversity. Various techniques that are based on utilizing the 16S rRNA gene are discussed. Of critical importance is the use of massively parallel sequencing to study bacterial diversity. Through massively parallel sequencing which is replacing traditional methods of bacterial identification; various bacterial habitats are surveyed to compile their species compositions.

عزل وتشخيص البكتريا المرضية مع ايلاء اهتمام خاص للبكترياStaphylococcus auerus المرافقة ل Cutaneous Leishmaniasis في محافظة ذي قار == Isolation and Identification Pathogenic Bacteri with Special Regard to Staphylococcus auerus Associate with Cutaneous leishmaniasis at Thi - Qar Province

Author name: رضاء الله مرتضى الاعظمي
Supervisor name: قاسم حسن وداعة
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Thi-Qar - College Of Science
Language: English
University location: Dhi Qar
First pages:
Abstract: The present study is designed to isolated and identification the pathogenic bacteria associated with Cutaneous leishmaniasis. Cutaneous leishmaniasis (CL) is a parasitic disease characterized by single or numerous ulcerations. This was study performed in Iraqi in Shatra general hospital and Al - hussein teaching hospital in Thi - Qar province 2016 - 2017.Total of 370 samples of suspected patients were taken and after examining them using geimsa stain , the positive were 186 males and 126 females then sterilize the area surrounding for ulcer , all samples were taken to (swab transport media) ,culture to MacConkey agar and blood agar after 24 hr of incubation at 37C ,after diagnosed by biochemical testing then species determined by API system. The positive results on culture media 92/312 , males 62 (67.4%) , female 30 (32.6 %).The total bacteria isolated from the lesions were in males and female staph auerus 52 cases (56.5%), Escherichia coli 8 cases(8.6%) , Bacillus cereus 6 cases(6.5%) , Pseudomonas. aeruginesa 5 cases (5.4% ), Strep. Pyogenes 6 cases(6.5%) ,Strepto.agalactiae 3 cases(3.3%).While other that isolated in males only and did not appear in females were aerobic bacteria ,Listeria moncytogenes 3 cases(3.4%) , Actinobacteria .spp 2 cases(2.2%) ,Klebsiella. Pneumonia 2 cases (2.2%) , Rodococcus .equi 1 cases (1.1%) , and anaeroberia bacteria ,Peptococcus niger 2 cases (2.2%) ,Peptostreptococcus .spp 2 cases (2.2%) .Then find bacteria that more presence more with cutaneous Leishmaniasis ,were it was staph. aureus therefore it were objected study antibiotic resistant .The present study demonstrates that Staph. aureus XIV isolates were resistant to methicillin 50 (96.15%), penicillin 48 (92.30%) , Clindamycin 37 (71.15%), ciprofloxacin 30 (57.69%) , Gentamicin 30 (57.69%),Amoxicalave 29 (55.76%), Azithromycin 25 (48.07%) , Amoxicillin 22 (42.30%), Tetracycline 21 (40.38%), Rifampin 10 (19.23%) and Vancomycin all staph .auerus are sensitive to Vancomycin The all 52 Staph. aureus isolates were further examined using polymerase chain reaction (PCR) for detection of (16SrRNA ,mecA and PVI gene ). 16SrRNA gene which were responsible for diagnosis ,where all isolates were positive to was 52\52 (100%) , mecA gene which were responsible for methicillin resistance this was detected in 52\52 (100%) . of Staph. aureus isolates and a part of virulence factors PVL gene was detected in 46/52(88.46%) of Staph. aureus isolates which are responsible to kill the leukocytes of human and other animals.

التوصيف المظهري والجزيئي للانواع البكتيرية المصاحبة لحالات الحروق في محافظة ذي قار == Phenotypic and Genotypic Charactrization of Bacterial species Assocuated with burn infections in ThiQar province

Author name: عبير هادي فرهود الحبيب
Supervisor name: رحمن لعيبي جلاب
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: هدفت الدراسة الحالية الى تشخيص الانواع البكتيرية المصاحبة لحالات الحروق والكشف الجزيئي عن الموروثه 16SrRNA في الانواع البكتيرية المشخصة واستخدام تقنية DNA Sequencing . تناولت الدراسة الحالية فحص ومعاينة 107 مريضا تم اختيارهم بشكل عشوائي (ذكور واناث) تراوحت اعمارهم بين 1 - 45 سنة من الراقدين في مستشفى الحسين التعليمي للمدة من حزيران - 2015 تشرين الاول 2016 اذ اجري الفحص السريري من قبل الطبيب المختص وتشخيص الاصابة على انها حالة حروق مرضية ناتجة عن اسباب مختلفة. جمعت 107 عينة مسحات قطنية Cotton swabs وزرعت على وسط اكار المكونكي ((MacConkey agar واكار الدم ((Blood agar والاكار المغذي ((Nutrient agar. شخصت العزلات عن طريق الاختبارات المظهرية والبايوكيمائية وعامل التشخيص API20E ونظام الفايتك ( (Vitek 2compactفضلا عن التشخيص الجزيئي باستخدام جين.16S rRNA واظهرت نتائج الدراسة الحالية وجود 117 عزلة بكتيرية تفوقت فيها العزلات السالبة لصبغة كرام ((Gram negative بنسبة 93.16% على العزلات الموجبة لصبغة كرام (Gram positive)بنسبة 6,83 وكانت نسب توزيع العزلات البكتيرية المشخصة Pseudomonas aeroginosa 32.47% , Klabseilla pneumonia 21.36% واقل نسبــة عزل كانـت لبكتريا Staphylococcus epidermidis بنسبة عزل 1.7% , في حين كانت نسب العزلات البكتيرية التالية Acinetobacter baumannii , Escherishia coli , . Enterobacter claocae ,Staphylococcus aureus , Burkholderia cepacia, Pruteus mirabillis, , Pantoea agglomerans بنسبة عزل. 12.36% , 11.1% , 5.12% , % 5.12 , 3.41% , 2.56 على التوالي.تناولت الدراسة الحالية بعض الجوانب الوبائية للحالات المصابة التي شملت معلومات عن المرضى المصابين بالحروق التي تضمنت الجنس , العمر , موقع السكن ونوع الحرق. وبينت النتائج ان عدد الحالات المشخصة كاصابات حروق للذكور كانت بنسبة 42.9% بينما كانت نسبة اصابة الاناث 57.%, تم تقسم المرضى الى عدد من المجاميع حسب الفئات العمرية. اعلى نسبة اصابة كانت في الفئة العمرية 9 - 1سنة بنسبة 54.2% وهي الفئة الخاصة بالاطفال تليها الفئة العمرية erishi29 - 20 بنسبة 20.56% واقل نسبة اصابة كانت في الفئة العمرية 49 - 40 بنسبة 4.67% وكانت نسب الفئات العمرية الاتية 19 - 10 , 39 - 30 بنسبة 11.21 , 9.34 على التوالي. اشارت النتائج الى ان حالات الاصابات المشخصة توزعت بنسب متفاوتة فيما يتعلق بنوع الحرق اذ كانت اعلى نسبة اصابة بالحروق بواسطة الماء المغلي Boiled water 38% تليها اصابة الحرق بواسطة لهيب الغاز Fire by gas flame بنسبة 28.9% واقل نسبة اصابة كانت لحروق الصدمة الكهربائية بنسبة 4.67%.اما فيما يتعلق بطبيعة ومكان السكن فقد اظهرت الدراسة ان اعلى نسبة اصابة كانت بين الافراد الذين يسكنون المناطق الريفية بنسبة 84.1% في حين كانت اصابات المركز بنسبة 15.88% . وجدت الدراسة الحالية ان نسبة الاصابة بالحوادث غير المقصودة كانت 86.9% في حين كانت الاصابات المقصودة 13% , لوحظ في الدراسة الحالية ان اعلى الاصابات بالحروق كانت تحدث في فصل الشتاء بنسبة 75.7% مقارنة باصابات الحروق في فصل الصيف 24.29% .اما بالنسبة الى اختبار فحص الحساسية فقد بينت نتائج الدراسة الحالية ان اكثر المضادات الحيوية فعالية ضد كل من البكتريا السالبة لصبغة كرامBacteria Gram negative والموجبة لصبغة كرام Gram positive هي Ceftazidin , Ciprofloxacin,Amicacin .واظهرت الدراسة ان اكثر العزلات مقاومة هي بكتريا P.aeruginosa التي اظهرت مقاومة لكل المضادات الحيوية بنسب متفاوتة. اكدت نتائج الدراسة الجزيئية لتضخيم جين 16SrRNA ان كل العزلات اعطت نتيجة موجبة لهذا الجين وان حجم الجين لكل الانواع البكتيرية كان 1500pb كذلك اكدت النتائج ان الحصول على500Pb من DNA Sequencing للجين 16SrRNA كافية لتشخيص الانواع البكتيرية كلا على حدة . ايضا تم مقارنة جميــــع نتائج DNA Sequencing للانواع البكتيرية (Pseudomonas aeruginosa ,Escherishi coli, Enterobacter cloaca , Proteus mirabillis, , Klebsiella pneumonia Staphylococcus.heamolyticus ,) التي اكدت تشابه نسب تتابع القواعد النيتروجينية مع نسب القواعد النيتروجينية المخزونة في البنك الجيني باستخدام برنامج MEGA6 Moleculas Evolutionary Genetics Analysis)) اكدت نتائج الدراسة الحالية على وجود 21 سلاله جديدة قيد التسجيل واعطيت الرمز TQU1 الى TQU12. | The present study addressed the examination and preview the 107 patients. who were randomly selected (males and females) and who ranged in age from 1 - 45 years old and have fallen asleep at the Hussein Teaching Hospital of the, period from Jon 2015 to November 2016. where he conducted clinical examination by a specialist doctor and diagnosed as a case of burns satisfactory result for different reasons. specimens were cultured on appropriated culture MacConkey agar, Blood agar and Nutrient agar, diagnosed isolates by phenotypic tests, Biochemical test, diagnosis factor API20E system Vitek 2 compact, as well as to molecular diagnostics, During this study, In total 117 bacterial isolates.109 isolates were Gram - negative bacilli and 8 were Gram - positive cocci. he distribution of bacterial isolates diagnosed Pseudomonas aeroginosa 32.47%, Klabseilla pneumonia 21.36% and the lowest rate was to isolate the bacteria by isolating Staph.epedermidis 1.7%, while the following bacterial isolates Acinetobacter baumennii, Escherishia coli, Enterobacter claocae, Staphylococcus .aueus, Burkholderia cepesa, Pruteus mirabillsi, Pantoea agglomerans by isolating : 12 : 82% , 11.1% , 5.12% 5.12% 3.41% 2.56 respectively. The present study addressed some of the epidemiological aspect of infected cases burns,which included information on patients with with burns, which included sex, age, residence location (place), burning type. The results showed that (42.9%) isolates were males and (57.%) were female.The most frequent patient were belonged to the 0 - 9 years old (54.2%) which is for children. 29 - 20 followed by isolation of 22 20.56% and less isolating rate was in the 49 - 40 age group. Created results also cases of infections diagnosed were distributed to varying degrees with respect to the type of cremation, where the highest rate of injury burns Boiled water 38%, followed by the burning by flames gas Fire 28.9%,Fire by gasoline flames (19.6%),Hot liquids (8.41%) and Electrical Shock(4.67%). As for the nature and place of residence have study found that the highest proportion of cases were among individuals who live in rural areas increased by 90 injured 84.1% while the injuries the center has reached 17 cases of burn injury by 10.88% ,Frequency of the manners of burning based on intention or accidentally was as follow : (86.95) of patients were burned accidentally and (13%) were intenion. Present results showed that the most frequent burning (75.7%)was occurred during winter. The lowest frequency (24.2%)was belonged to summer .So the most common time of burning of present was during winter. As for the screening test sensitivity was shown the results of the current study that the most effective antibiotics against both Gram - negative bacilli bacteria and Gram - positive cocci were Ceftazidin, Ciprofloxacin, Amicacin while most of isolates showed high resistance to Ticarkcillin, Gentamicin, Impenem. Where the highest proportion of resistant bacteria P.aeruginosa which showed high resistance to all antibiotics in different proportions. It showed the results of the molecular study to amplify the gene 16SrRNA that all isolates gave a positive result and it's within the range 1400pb - 1500 pb. Also it has been compared to the results of all the DNA Squencing bacterial species (P.aeruginosa, E.coli, E.cloaca, A.baumennii, P.mirabili), which confirmed its similarity ratios nitrogenous bases relay with nitrogenous bases ratios stored in the gene bank

التوصيف الجزيئي لبعض الفطريات الانتهازية المعزولة من المرضى الضعاف مناعيا واختبار حساسيتها تجاه مستخلص الثوم == Molecular Identification of Some Opportunistic Fungi Isolated from Immunocompromised Patients and Their Testing Sensitivity Against Extract of Garlic ( Allium sativum)

Author name: هند حبيب عبد الامير الفرطوسي
Supervisor name: ياس خضير عباس | ميثاق ستار عبود
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: تضمنت الدراسة الحالية العزل والتوصيف الجزيئي والمظهري لبعض الفطريات الانتهازية من القناة التنفسية السفلى للمرضى الضعاف مناعيا المراجعين لقسم الاورام في مستشفى الحبوبي والمراجعين للعيادة الاستشارية للامراض الصدرية في محافظة ذي قار (جنوب العراق) حيث تم جمع 130عينة قشع sputum للفترة من 1/10/2015 الى 1/5/ 2016 . بلغ عدد العينات الموجبة للزرع 85عينة وبنسبة 65.38% , اذ شكل عدد عينات الاناث الموجبة 21 عينة وبنسبة 46.67 % , اما عدد عينات الذكور الموجبة فقد بلغ 64 عينة وبنسبة 75.29% . ظهرت اعلى نسبة عزل عند الفئة العمرية 61 - 70 سنة وبنسبة 32.94 % بعدها الفئة العمرية 31 - 40 سنة وبنسبة 15.25 % . وتم الحصول على 106 عزلة فطرية . باستخدام طرق التشخيص المظهرية والجزيئية, بينت هذه الدراسة ان اعلى نسبة ظهور للفطريات المعزولة تمثلت بجنس spp. Candidaثم جنس Aspergillus spp.تلاه جنس Penicillium spp. بالنسب المئوية 49.41% 38.32 % 21.17 % وبالترتيب . كما ظهرت Candida albicans ثم Aspergillus flavus بالنسب المئوية 36.47 % , 12.94% بالترتيب, كما تم تسجيل 15 عزلة من الفطريات المعزولة في البنك الجيني الدولي . شملت الدراسة استخدام اربعة مذيبات عضوية وهي الايثانول, الاسيتون, الميثانول والماء بحيث كان قد استخدم من المذيبات الثلاثة غير الماء التراكيز 50%, 70% فضلا عن التركيز المطلق للحصول على مستخلص الثوم. اختبرت اربعة تراكيز من كل مذيب ضد 11 نوعا من الفطريات المعزولة, واظهر المستخلص المائي كفاءة جيدة في تثبيط جميع انواع الفطريات المدروسة بالمقارنة مع المذيبات العضوية الاخرى اذ بلغ اعلى معدل تثبيط عند التركيز400 مايكروغرام\مل بحيث ابدى الفطر Penicillium notatum تاثرا كبيرا للمستخلص المائي الذي بلغ اعلى معدل تثبيط له (60 مليمتر) عند التركيز المذكور . حددت الفعالية الحيوية لكل مذيب ضد كل الفطريات المدروسة وتم مقارنة المستخلص مع ثلاثة مضادات فطرية Miconazole وNystatin وAmphtericin B واظهر المضادان الاول والثاني تاثيرات متباينة اما المضاد الثالث فلم يظهر اي تاثير على الفطريات المختبرة . تم تحديد السمية الخلوية للمستخلص المائي الخام باستخدام كريات الدم الحمر ولم يتسبب في اي تحلل دموي. كذلك تضمنت الدراسة الكشف عن المركبات التي يحتويها المستخلص المائي للثوم باستخدام الكشوفات النوعية وتقنية كروماتوغرافيا الغاز GC فكشفت تلك الطرائق وتقنية كروماتوكرافيا الغاز بان المستخلص يحتوي على القلويدات والفينولات والفلافونويدات والتربينويدات والتانينات والكاروتينات بالاضافة الى وجود الاحماض الامينية ومركبات كبريتية اهمها الاليسين allicin واخرى اروماتية | The current study included isolation and molecular and phenotypic identification of some the opportunistic fungi from the lower respiratory tract of the immunocompromised patients in the Department of Oncology and Thoracic Consultant Clinic of Alhaboobi hospital at Thi - Qar Province ( South of Iraq). The specimens of sputum were 130 which collected during 1/ October /2015 until 1/May/2016. The study showed that 85 of the collected specimens appeared as positive results which were 65.38% of the ratio in which the females ones were 21 (46.67%) specimens while 64 (75.29%) for males. In addition that, the highest ratio of the positive results was recorded in the ages of 61 - 70 years, followed by 31 - 40 years which were 32.94% and 15.25%, respectively. Totally, 106 isolates of the isolated opportunistic fungi appeared from the positive specimens. Through using morphological methods and molecular techniques, this study showed the first rank of the isolated fungi was Candida spp followed by Aspergillus spp, and Penicillium spp which possessed ratios of 49.41%, 38.32% and 21.17, respectively. According to the species, the first ratio of the isolation was Candida albicans followed by Aspergillus falvus, as 36.47%, 12.94%, respectively. Fifteen fungal isolates of the present study were recorded in the Gene Bank of NCBI. In this study, the extracts of Allium sativum were separately obtained by using four organic solvents (ethanol, acetone, methanol and water) and three concentrations (50%, 70% and absolute) of each solvents except water. Four concentrations of each extract were tested against 11 species of the isolated fungi in which the water extract gave the best value of the bioactivity including highest inhibitory value (60 mm) against Penicillium notatum by using 400µg\ml compared with the extracts of ethanol, acetone, and methanol. The bioactivity of Allium sativum extracts of each solvent was done against the tested fungi in comparison with Amphotericin B, Nystatin and Miconazole in which two of them produced the different bioactivities while Miconazole had no effect. The blood cytotoxicity of the crude water extract was performed against red blood cells and it did not appear any hemolysis. The chemical tests and Gas Chromatography (GC) revealed that the water extract contains alkaloids, phenols, flavonoids, terpenoids, tannins and carotenes in addition to sulfur formations, such as allicin as well as aromatic compounds.

التحري الجزيئي والوبائي لطفيلي المتحولة Entamoeba Spp. في الاطفال المصابين بالاسهال في محافظة ذي قار == Molecular and Epidemiological investigation of the Parasite Entamoeba Spp. In children with diarrhea in Thi Qar Province

Author name: منار كريم كاظم السعيدي
Supervisor name: فاضل عباس منشد العبادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: شملت الدراسة الحالية فحص 3909 عينة غائط للاطفال دون سن 12 سنة الذين يعانون من الاسهال ويشكون من الام بطنية والمراجعين لمستشفى بنت الهدى التعليمي ومستشفى الشهيد محمد الموسوي والمراكز الصحية والعيادات الخارجية في محافظة ذي قار خلال المدة من شهر تشرين الاول 2015 لغاية شهر ايلول 2016 تضمنت الدراسة ثلاثة محاور رئيسة . المحور الاول هو تشخيص جنس المتحولة مجهريا باستعمال المسحة المباشرة الرطبة وطريقة التركيز وذلك للكشف عن الاطوار المتغذية والمتكيسه للطفيلي واظهرت اصابة كلية بجنس المتحولة Entamoeba spp. بنسبة 26.86% واختيرت 80 عينة من العينات الموجبة مجهريا لتاكيد التشخيص باستعمال الفحص الجزيئي PCR بواسطة الجين 18S rRNAفسجلت الدراسة اصابة كلية بطفيلي المتحولة بنسبة 35% ، اظهرت نتائج الفحص باستعمال طريقة Nested PCR لتمييز الانواع (Species Specific) اصابة بطفيلي المتحولة الحالة للنسج Entamoeba histolytica بنسبة 39.29% وبطفيلي المتحولة المتغايرة Entamoeba dispar بنسبة 39.29% . فحصت العينات الموجبة (28) للPCR باستعمال Real - Time PCR وبوجود المجسان Probes المجس Histolytica - 96 T الخاص بالمتحولة الحالة للنسج والمجس Dispar - 96 T الخاص بالمتحولة المتغايرة وذلك لتاكيد التشخيص وتمايز الانواع فثبت وجود المتحولة الحالة للنسج باعلى نسبة 53.57% والمتحولة المتغايرة بنسبة 39.29% والانواع الاخرى لجنس المتحولة بنسبة 7.14% وبفارق معنوي بين الطريقتين الجزيئيتين مع وجود تاثير معنوي لنوع الطفيلي في احداث الاصابة . اظهرت الدراسة اصابة مفرد لطفيلي E. histolytica بنسبة 45.50% ولطفيلي E.dispar بنسبة 27.20% فيما سجلت اصابة مختلطة بنسبة 27.30% كما وسجلت وجود كريات الدم الحمر في 80% من العينات الموجبة لوجود E. histolytica وبنسبة 54.55% من العينات الموجبة لوجود E.dispar . تضمن المحور الثاني دراسة انتشار الاصابة بجنس المتحولة من خلال دراسة علاقة الاصابة مع بعض العوامل الوبائية ، منها فصول السنة التي اظهرت وجود فروق معنوية P≤0.05 فكان فصل الصيف اعلى الفصول من حيث نسبة الاصابة 37.06% وادناها في فصل الشتاء بلغت 14.14% ، كذلك لم تبين النتائج وجود فروق معنوية للاصابة فيما يخص الفئات العمرية فكانت النسب اعلاها بين الاطفال دون (3) سنوات فبلغت 30.50% ادناها بين الاطفال في الفئة الاعلى من (10) سنوات بلغت 17.13% ، لم تسجل الدراسة اية فروق معنوية في نسبة الاصابة بالطفيلي بحسب الجنس فسجلت نسبة 26.87% و26.85% للاناث والذكور على التوالي، في حين اظهرت الدراسة وجود فروق معنوية ملحوظة فيما يخص المستوى المعاشي فكانت نسبة الاصابة لدى الاطفال الذين ينتمون للعائلات ذات الدخل المحدود هي الاعلى 37.98% واقلها للذين ينتمون للعائلات ذات الدخل فوق الجيد وبلغت 10.11% كما سجلت النتائج وجود فروق معنوية في نسب الاصابة حسب المستوى التعليمي للابوين فكانت نسبة الاصابة عالية لدى الاطفال الذين المستوى العلمي لذويهم دون الابتدائية مقارنة بذوي الاطفال من حملة الشهادات الجامعية وبلغت النسب 36.85% و8.55% على التوالي فيما يخص عدد افراد العائلة كانت نسبة الاصابة عالية وبفارق معنوي لدى الاطفال الذين ينتمون لعائلات تتكون اكثر من (10) افراد واقلها للذين ينتمون لعائلات التي يبلغ عددها (5 - 3) افراد بنسب بلغت 36.95% و8.54% على التوالي . تضمن المحور الثالث دراسة بعض المعايير الدمية ومعرفة التغير في نسبة هذه المعايير لدى الاطفال المصابين بالطفيلي عند مقارنتها بالمعايير الدمية للاطفال الاصحاء وتم ذلك باستعمال جهاز Coulter Counter . جمعت 150 عينة دم من الاطفال المصابين بطفيلي المتحولة و50 عينة دم من الاطفال الذين يمثلون مجموعة السيطرة الاصحاء وتبين وجود فروق معنوية واضحة في نسبة خضاب الدم 10.16%وحجم كريات الدم الحمر المضغوط 34.7% حيث لوحظ انخفاضهما في مجموعة المصابين مقارنة بالقيم في مجموعة السيطرة التي بلغت 12.34 و39.10 على التوالي. سجلت نتائج العد الكلي لخلايا الدم البيض وجود ارتفاع معنوي في الاطفال المصابين مقارنة بمجموعة الاطفال الاصحاء وبلغت 11.3، 8.37 على التوالي وفيما يخص العد التفريقي لها فقد بينت النتائج وجود ازدياد بفارق معنوي في عدد الخلايا العدلة 6.92 و4.03 والخلايا الحمضة 0.61 و0.17 والخلايا الوحيدة 1.19 و0.93 على التوالي ولوحظ كذلك وجود انخفاضا معنويا في عد الخلايا اللمفاوية في الاطفال المصابين مقارنة بقيمتها لدى اطفال مجموعة السيطرة التي بلغت 2.19 و2.76 على التوالي | The current study included examination of 3909 stool samples from under 12 years old children who visited Bint Al - Huda hospital, Martyr Mohammad Al - Mosawi hospital, medical centres and outpatient clinics in Thiqar governorate during the period from October 2015 until September 2016. All those individuals were suffering from diarrhea and abdominal pain. This study included the microscopic identification of Entamoeba Spp. using direct wet amount and concentration techniques in order to diagnose both Trophozoite and cyst stages of the parasite. The result revealed that 1050 (26.86%) of the collected samples were infected with Entamoeba Spp. Out of these infected samples, 80 were randomly selected for further confirmation by conventional PCR and 18S rRNA. Using this method, 28 (35%) was recorded to have Entamoeba Spp. Positive samples from conventional PCR were further examined by Nested PCR for species specific identification. The result of this method showed the presence of two species, E. histolytica (39.29%) and E. dispar (39.29%).Positive samples for molecular examination (28) were then examined by Real time PCR in order to verify and confirm the molecular results. Two probes were used, Histolytica - 96 Tfor E. histolytica and Dispar - 96 T for E. dispar. A significant difference was observed between two methods. E. histolytica was found to be higher percentage followed by E. dispar and then Entamoeba Spp., 53.57%, 39.29% and 7.14% respectively. The Real time PCR reported mono - infection with E. histolytica in 45.5% and with E. dispar in 27.2%, whereas reported mixed infections by both parasites in 27.3%. Red blood cells (RBCs) were detected in 80% E. histolytica positive samples and in 54.55% E. dispar positive ones.The environmental factors and their correlation with Entamoeba Spp. prevalence were also included in this study. The infection prevalence based on the seasonal variation showed significant differences P≤ 0.05. Summer recorded the highest infection prevalence whereas the lowest was in the winter, 37.06% and 14.14% respectively. Regarding the infection in age groups, the highest percentage (30.5%) was recorded in under 3 years old group and the lowest (17.13%) was found in above 10 years old one. However, no significant difference P≤ 0.05 was observed between these age groups. Furthermore, no significant differences were recorded betweenpatient’s sex. Entamoeba Spp. infection (37.98%) appeared to be higher in children who belong to families with low standard of living compared to those who are from families that earning a higher income (10.11%). A Similar pattern was found in Entamoeba Spp. prevalence based on the number of the patient’s family members. Higher prevalence and significant difference were reported (39.95%) in patients who belong high family member (above 10), compared to those from 3 - 5family member (8.54%).One hundred and fifty blood samples from infected children with Entamoeba Spp. and fifty samples from healthy children as a control were collected during the present study in order to observe the change in blood picture and parameters between two groups. Significant differences were reported in blood hemoglobin (10.16%) and volume of packed RBCs (34.7%) in the infected group compared to 12.34% and 39.10% in the control group. Total leukocyte count was found to be significantly higher in the infected group (11.3) compared to the individuals in the control group (3.37). Differential leukocyte count also showed significant differences in Neutrophils (6.92%, 4.03), eosinophils (0.61, 0.17), monocytes (1.19,0.93) and lymphocytes (2.19, 2.76) in infected and control groups respectively.

تاثير الاصابة التجريبية بطفيلي المقوسة الكوندية Toxoplasma gondii وبعض الادوية على خصوبة الجرذان المختبرية Rattus norvegicus == Effect of experimental Toxoplasma gondii infection and some drugs on fertility rats Rattus norvegicus

Author name: زهراء سعدون هادي الغزي
Supervisor name: فاضل عباس منشد العبادي | علي اسماعيل عبيد السنافي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: This study included 3 stages. In the first stage, Toxoplasma gondii was isolated from aborted women placenta who visited Bent Al - Huda hospital and Al - Shatra hospital after confirmation of the prescence of parasite by impression smear method. Then parasite uspension injected 0.3 ml which contain 100 cyst intraperitoneally (174 rat) to 87 male and 87 female rats, inaddition to control group 30 rat (15 male and 15 female). After 10 days of injection, diagnosed the acute infection by Real - Time Poplymerase Chain Reaction(Real - Time PCR) and showed 97.70% (170 infected rat and 4 noninfected rat) , in male 96.55% (84 infected rat and 3 noninfected) ,in female 98.85% (86 infected rat and 1 noninfected) . After 2months of infection 84 animals (42 males and 42 females)were sacrificed, dissected (brain, testis and ovary ) were isolated to confirm the occurance of chronic infection with the using Real - Time PCR. The incidence of infection was 100% in brain males and ovary females and recorded 71.43% in testis and 90.48% in brain females. So diagnosed infection by impression smear method, identified the amount of DNA of the parasite T. gondii by using Real - Time PCR. The use of the remaining number of animals within the following experiments.The second stage included effect of experimental Toxoplasmosis infection and some drugs on fertility 168 rat (84 male and 84 female). Animals were subdivided into 6 groups for each group included 14 rat : (First group) non infected treated by DMSO, (Second roup) non infected treated by Sulfadiazine, (Third group) non infected treated by Pyrimethamine, (Fourth group) infected treated by DMSO, (Fifth group) infected treated by Sulfadiazine and (Sixth group) infected treated by Pyrimethamine.The current study results revealed prominent effect of T. gondii infection on the hormonal level in both males and females. T. gondii infection induced significant decline in Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH) and Testosterone levels in nfected male compared with non infected males treated by DMSO. Sulf. and Pyr. treatment significantly elevated the level of these hormones (P <0.01) in infected males compared with fourth group similar to levels its in first group. Toxoplasmosis in female rats significantly decrease FSH, LH, Estrogen and Progesterone levels in compared with first group, while treating of infected females with Sulf. and Pyr. didn,t improve the declined levels of these hormones in infected and noninfected females comparedwith fourth and first group, respectively.The study showed that Toxoplasmosis in male rats induced significant decline in the sperm count in the epididymal cauda, sperm viability and relative weights of male sexual organs with an increase in sperm malformation ratio in infected male compared with first group. Treatment with Sulf. and Pyr. in infected male significanthy elevated the sperm count and viability (P<0.05), compared with fourth group. Sulf. didn,t induced further significant changes, but Pyr. induced further significant increase in sperm malformation ratio in infected (P< 0.01) and non infected (P<0.05) rats. The study also revealed that Toxoplasmosis significantly decrease the relative weight of ovary and uterus compared with first group, while treatment with Sulf. and Pyr. increased the declined relative weight of the ovary and uterus in the infected females.Testicular histological section of Toxoplasmosis infected male rats treatment with DMSO showed vacular degeneration of spermatogonia and spermatocytes. The seminiferous tubules revealed sever degeneration, shrinkal, necrosis, hemorrhage. Seminiferous tubules lumen contained little amount of sperm with appearance of gaint cell, polymorphonuclear leukocytes and exfoliated cellular debris. Metamorphosis and disintegration of the tissue between the seminiferous tubules was also observed compared to the first group that showed complex histologically intact while testes sections of infected rats treatment Pyr. and Sulf. showed improvement by removing histocompatibility damage caused by the parasite infection.The epididymal sections of T. gondii infected rats treatment with DMSO showed infiltration of mononuclear and multinucleated gaint cell. Sections also showed hyperplasia of duct lining and contained tissue cyst of T. gondii. All these changes were improved in infected males treated by Sulf. and Pyr. Ovary sections of the infected females treatment with DMSO compared with first group revealed decreased primary and secondary follicle increased follicle atresia. Histological sections of ovary females showed in the fifth and sixth group to increase the number of primary and secondary follicles and decreased number of follicle atresia compared overian sections for the fourth group. Uterine sections of the infected females treatment with DMSO showed hypertrophy, increase endometrial thickness and myometerium, increase polymorphonuclear cell infiltration, fewer glands and congestion of blood vessels compared with first group. Histological sections for ovary females of the fifth and sixth group showed changes but less severe than is present in the fourth group.In third stage from studying Toxoplasmosis on fertility parameters of male and female rats, 84 rat(42 male and 42 female) which showed the results of the current study, when paired fertilized healthy females by males is infected and infected of T.gondii treatment with Sulf. , Pyr. and DMSO, and inversion, there is the effect of the parasite and used drugs the percentage of getting pregnant and pregnancy size and rate of embryos weight and placental weight and increase the proportion of fetal resorption and the proportion of early loss of embryos in fertilized healthy females by males five groups compared to the first group.Based on that conclude from the study that the infection of parasite T. gondii had negative effects on the fertility of male and female, and that all of thePyr. and Sulf. were not honest is the other of the impact on reproductive functions.

جدولة n من النتاجات على ماكنة واحدة لتصغير دالتين == An n - Jobs One Machine Scheduling for Minimization the Sum of Two Criteria

Author name: ازهار مهدي عبادي
Supervisor name: محمد كاظم زغير الزويني
General topic: Mathematics
Specific topic: Mathematics
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Mathematics
Language: English
University location: Dhi Qar
First pages:
Abstract: In this thesis, we considered the problem of scheduling ???? jobs on a single machine. The aim of this study is to find the optimal and near optimal solutions for the sum of cost of total flow time and maximum late work with unequal ready time, this problem denoted by 1????????⁄Σ????????+????????????????????????=1⁄. The problem is strongly NP - hard, the branch and bound method was using to find optimal solution. Two lower bounds (LB1, LB2) are proposed each of them based on decompose the problem into two sub problems. The lower bounds of the problem is the sum of the lower bounds of the two sub problems. A heuristic which gives an upper bound in the root node of BAB algorithm was proposed, its effective in finding an optimal or near optimal schedule. Also, we proved some special cases of the problem which lead to optimal solution, three dominance rules were stated and proved. The results of extensive computational tests show that the proposed BAB algorithm is effective in solving problems up to (35) jobs at a time less than or equal to (30) minutes.We apply two local search methods to find near optimal solutions : Artificial Fish Swarm Algorithm (AFSA) and Fruit Fly Optimization Algorithm (FOA) .Computational experience found that these local search methods can solve the problem up to (6000) jobs with reasonable time, also found that : The AFSA has better results for the problem of size less than or equal to (35) jobs, but for the problems of size larger than (35) jobs, The FOA has the best results. All methods used in this research are programmed by using a programming language (MATLAB Language).

Blow up method for studying bifurcation of solution in singular perturbation ordinary differential equation and differential algebraic equation

Author name: حوراء كريم مناهي
Supervisor name: كمال حامد ياسر الياسري
General topic: Mathematics
Specific topic: Mathematics
Degree: Master
University: University of Thi-Qar - College Of Education For Pure Sciences - Department Of Mathematics
Language: English
University location: Dhi Qar
Key words:
  • Blow up Method
  • Bifurcation Theory
  • Singular perturbation system Di erential - Algebraic Equations
First pages:
Abstract: This thesis deals with studying a new subject of a singularity perturbed ordinary di erential equations system. It is considered the foundation base to get a di erential algebraic equations, where the concept of singularity perturbed ODEs is explained. Thenit studies the ways to deal with the perturbation parameter ϵ through two case studies : The rst case : Is the study of behavior of solution for singularity perturbed ODEs when perturbation parameter 0

تشخيص تصلب الشرايين باستخدام معالجة الصور الرقمية == The Diagnosis of Atherosclerosis Using Digital Image Processing

Author name: عبد الكريم صكر كمر
Supervisor name: حازم باقر طاهر العلي
General topic: Physics
Specific topic: Physics
Degree: Master
University: University of Thi-Qar - College Of Science - Physics Department
Language: English
University location: Dhi Qar
First pages:
Abstract: Accelerating the pace of scientific progress and that focus in the field of human service requirements is to provide daily health and service. Health aspect is one of the most important themes of science that needs to be searched because it has a direct impact on human existence. Medical Physics is one of the branches of the Physics Department that deals with the interest of providing theoretical or scientific foundations which had the aims to provide scientific services in the medical field. The subject of the medical image processing is one of the applications of this section. It aims to increase the efficiency of medical diagnostic systems to provide an integrated diagnostic system that works to distinguish a heart disease and atherosclerosis.This study depends on medical images that were obtained from patients in Nasiriyah Heart Center. Where thesis focus on images for arteries feeding heart muscle for Infected patients and other healthy. Set of operations were performed on these images derived from work areas and the separation arteries of these areas from the rest of unimportant information within the image To be brought into the neural network, the use of 110 image to train the network 60 and 50 were used for the test.The next step is to count the number of qualities for each image after it has been extracted artery of the image by making some operations on this image .The proposed system has proven successful in the conduct of the diagnostic process through the results obtained on the ratio of 100% in the training phase and 92% in the testing phase

دراسة وتحضير خلية شمسية صبغية ذات تركيب صلب باستخدام صبغة نوع D146 ثنائي اوكسيد التيتانيوم كانود وانابيب الكاربون النانوية متعدد الجدار ككاثود == Study and Preparation of Solid State Dye Sensitized Solar Cell using D149 as a dye ,TiO2 Nanoparticles as a Photo anode and MWCNTs as a Counter Electrode

Author name: ايات عبد علي كطان
Supervisor name: سامر مهدي عبد المحسن
General topic: Physics
Specific topic: Solar Energy Physics
Degree: Master
University: University of Thi-Qar - College Of Science - Physics Department
Language: English
University location: Dhi Qar
First pages:
Abstract: The dye sensitized solar cell (DSSC) is one of the photochemical electric cells, which consists of the photo electrode, dye, electrolyte, and the counter electrode. The advantage of DSSC is the low cost of the solar energy conversion into electricity because of inexpensive materials and the relative ease of the fabrication processes. In this work, titanium dioxide (TiO2) films as a photo electrode were prepared from anatas titanium Oxid (TiO2) nanoparticles and deposited by doctor blade method on fluorine doped tin oxide (FTO)coated glass substrates. After the process of deposition that including the prepared samples in the oven, the temperature raises from room temperature (R.T) to set up to around 400°C. Half an hour later, the temperature started to decrease to reach theroom temperature (R.T) . Multi - walled carbon nanotubes (MWCNT)used as a counter electrode instead of platinum(Pt),where prepared by spray pyrolysis technique (SPT) at 300°C,10 sec as spraying time ,and 60 sec stopped time. In the next step, Preparing the dye solution at room temperature then placed TiO2 electrode in the dye solution for 24 hours in the dark .The last step is collecting two electrodes one over the other leads to build a solar cell. Then a mixture electrolyte about(0.6 M 1 - methyl - 3 propoylimidazolium iodide (PMII) + 0.1 M LiI )is dipped onto the surfaces of both (FTO glass/TiO2NP/D149/PMII and MWCNTs/FTO)glasses .The thickness about (400nm) and the films are prepared under various deposition conditions in order to understand the effect of the temperature on the cell properties and to specify the optimum condition. X - ray diffraction (XRD), scanning electron microscope (SEM) are used for the structural analysis.Raman scattering spectrum used to determine the type of material CNTs used for DSSCs fabrication , Fourier transform infrared spectroscopy (FTIR) used for description as an analysis for system chemical structure of the atoms. UV and visible spectra have been recorded as a function of wavelength in the range (200 - 1000 nm) in order to study the optical properties such as reflectance, energy gap of allowed and forbidden direct transitions, extinction coefficient, refractive index, and dielectric constant in real and imaginary parts. It has been found that all the investigated parameters are affected by the sensitizer dye in the visible region of the spectrum .Finally I - V characteristic curves of the fabricated cell were measured and studied, this cell shows highest photovoltaic performances to date as Solid state dye sensitive solar cells
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