Author name:
منى خزعل جابر القطراني
Supervisor name:
عباس مهدي جاسم | منال زباري سبتي
Abstract:
A study was conducted at the technology tissue culture lab that belong to private sector in Fayhaa / Shatt al - Arab / Basra province and tissue culture lab of college of Agriculture / University of Basra from November 2014 to December 2015, off shoots of Musa acuminate dwarf banana cv. Indian of six months age were used as a source of explants which was propagated by half of Shoot tips on MS medium supplement with Benzyl adenine (BA) to stimulate shoot multiplication and then rooting on medium free of Cytokinin and supplemented by Naphthalene acetic acid NAA at 1 mg . L - 1, Then Plantlets were hardened by two types of covering (Polyethylene cover and glass cover) to find its effect on success of hardening . plants then transferred to greenhouse and treated by two types of NPK fertilizer (NPK+ TE) 20 - 20 - 20+TE and 10 - 20 - 30+TE by four levels : 0.5, 1.0, 1.5 and 2.0 gm .plant - 1 besides the control treatment (without fertilization). Fertilizers were added after two and four months of hardening to find the effect of those fertilizers and interference between them in addition after two months and four months later on vegetative growth and chemical characteristics of banana plants derived from tissue culture. results can be summarized as follows : 1 - Results showed no significant difference between the two types of covering in percent of success of hardening , percent of lost humiditiy and vegetative growth except plant height where glass cover was more effective than polyethylene cover.2 - The results showed the effect of fertilizer type on vegetative growth of banana plants where the NPK (20 - 20 - 20+ TE) ( F1) was superiverالخلاصة SUMMARYBcompared to NPK (10 - 20 - 30+ TE) (F2) in most of vegetative growth (leaf area , plant height, stem diameter) which were (82.6 cm 2, 28.74 cm, 12.16 mm) respectively fo r the first addition and (123.7 cm 2, 34.07 cm, 14.33 mm) respectively for a second addition, where was no significant difference in number of leaves under the two types of fertilizers .3 - Fertilizer level at 2.0 gm .plant - 1 was significantly more effective in leaf area, plant height and stem diameter for both additions and (108.5 cm 2, 31.67 cm, 14.10 mm) respectively for the first addition and (190.8 cm 2, 39.40 cm, 17.13 mm) for a second addition respectively, whereas, 1.5 gm .plant - 1 was superior leaves number for both addition .4 - There were interaction between type and level of fertilizer ,where F1 type and 2.0 g . plant - 1 gave highest leaf area, plant height and stem diameter for both addition which were 121.3 cm 2 , 34.42 cm and 15.19 mm respectively for the first addition and 212.1 cm 2, 41.46 cm and 17.96 mm for a second addition respectively. The interaction between fertilizer type F1 and 1.5 g . plant - 1 level was superior in leaves number for both addition which were 3.40 and 6. 00 laef . plant - 1 respectively5 - Fertilizer type F1 was more effective than type F2 on chlorophyll a , b and total chlorophyll, (0.647, 0.367, 1.014) mg. gm - 1 respectively for addition after two month and (0.731, 0.403, 1.134) mg. gm - 1 respectively for addition after four months later. Fertilizer level at 2.0 gm . plant - 1 was superior in chlorophyll content for both additions after two and four months .6 - The interaction between fertilizer type and fertilizer level was significant effect on the stock content of chlorophyll a , b and total chlorophyll forالخلاصة SUMMARYCboth addition, where F1type and 2.0 g . plant - 1 level ,gave the highest rate (1.098, 0.546, 1.644) mg . gm - 1 respectively for the addition after two months and (1.160, 0.574, 1.734) mg .gm - 1 respectively for the after four months addition .7 - No significant effect for the fertilizer types of carbohydrate , and phosphorus content for both addition, and fertilizer type F1 was more effective than type F2 on nitrogen content for both additions and rate of (27.71, 32.25) mg. gm - 1 respectively . F2 type was superior of potassium content for both additions and give (23.92, 29.10) mg. gm - 1 respectively8 - Results showed the level of fertilizer 2.0gm.plant - 1 was significant effect in carbohydrates, nitrogen, phosphorus and potassium level (54.09, 33.57, 2.13, 25.57) mg. gm - 1 after two months addition and (62.51, 39.95, 2.37, 31.37) mg . gm - 1 respectively for the addition after four months .9 - The level interaction between F1type of fertilizer and 2.0 g . plant - 1 level was on carbohydrates and nitrogen (55.35, 35.34) mg. gm - 1 after two months and (63.13, 42.53) mg. gm - 1 respectively for the four months addition .10 - F2 type and 2.0 gm. plant - 1 level give the highest phosphorus level (2.20) gm. gm - 1 after two months addition , while for added after four months may be greater than F1type and 2.0 gm. plant - 1 level a significant Superiority at over all interactions highest rate (2.48 mg .gm - 1) . As for the content of potassium the leaves , F2 type and 2.0 gm. plant - 1 level was significant and give the highest rate (26.10, 33.70) mg. gm - 1 for two addition respectively.
