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دراسة وبائية على طفيلي المشعرات المهبلية Trichomonas vaginalis وبعض الجراثيم المسببة للامراض المنقولة جنسيا وتاثير بعض المستخلصات النباتــيـــــة على نمو الطفيلي في الزجاج في مدينة كركوك == Epidemiological Study On Trichomonas Vaginalis & Some Associated Bacteria That Causing Sexual Transmitted Diseases & Effect of Some Herbal Extraction On The Parasite In Vitro In Kirkuk City

Author name: محسن عز الدين سليمان
Supervisor name: ابراهيم شعبان داوود | محمد عبد العزيز قادر
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: بلغ العدد الكلي للعينات التي جمعت وفحصت للفترة من بداية شهر تموز 2007 الى نهاية شهر مايس 2008 (2345) عينة لكلا الجنسين مــن مستشفيات كركوك للتحري عن وبائية طفيلي المشــــــعرات المهبليـــــــــة Trichomonas vaginalis وبعض الجراثيم المسببة للامراض المنقو | A total (2345) specimens were collected & examined from both sexes, from the beginning of July 2007 till the end of May 2008 attending Kirkuk hospitals for epidemiological study of Trichomonas vaginalis & some sexual transmitted microorganisms. The vaginal examinations were done by gynecologist, two high vaginal swabs were taken from (250) women & a cervical swab from some women with vaginal discharge. General urine examination & blood group with Rh factor were performed for each woman with vaginal discharge. Direct microscopic examination, staining & culture of swabs were performed on different methods for diagnosis of Trichomonas vaginalis, followed to diagnose Candida albicans, Neisseria gonorrhoeae, Chlamydia trachomatis & Treponema pallidum as well as some other bacterial groups. The rate of Trichomonas vaginalis was (2.8%) & Neisseria gonorrhoeae (0.8%), while no Chlamydia trachomatis & Treponema pallidum recorded in this study. The highest rate of Trichomonas vaginalis was among the age group between 15 - 29 years (3.6%), the rate of Trichomonas vaginalis infection was higher among illiterate (5.7%) than educated ones. The highest rate of Trichomonas vaginalis was recorded among those with blood group O & Rh (+ve) (3.8%). The same result was found regarding other microorganisms in both sexes. Statistically the results were not significant.The rate of infections in females was higher than males in all infection except Neisseria gonorrhoeae was recorded highest rate in males(35%). The percentage of mixed infections with Trichomonas vaginalis was Candida albicans (1.6%) & B - hemolytic streptococcus (0.4%). The maximum infections rate in vaginal swabs were Staphylococcus aureus (42.3%) & lowest rate of Neisseria gonorrhoeae (2%). Regarding the distribution of microorganisms isolated from urine samples of females suffering from vaginal discharges, the highest rate of infection was Eschirechia coli (22.2%) & lowest rate of Neisseria gonorrhoeae (2%) respectively. The rate of isolated microorganisms from urethral discharge of males were Neisseria gonorrhoeae (35%) & Escherichia coli (5%). Antibiotics Ciprofloxacin & Amikacin showed good inhibition activity against all pathogenic microorganisms. Regarding the effect of different concentration of metronidazole & medical herbs to the Trichomonas vaginalis invitro, metronidazole showed clear effective inhibition at concentration (1.25%) during 24 hours. The inhibitory effect of Zea mays (both alcoholic & watery extractions) was greater than Apium graveolens & Foeniculum vulgare. At concentration (2.5% & 5%) of Zea mays in both extractions was almost identical to metronidazole. Six solvents were used by thin layer chromatography (TLC) in order to separate alcoholic & watery extraction of medical herbs, only three of them A, B & F gave good results in separating herbal extraction with differing in its component. By measuring relative flow rate (Rƒ) of all compounds that separated and determind. The chemical compounds of each herbal extract were established & included alkaloids, flavonieds, amines, pigments, oils, carbohydrates, phenols, purines & tanines.

التحري عن الاميبا الحالة للنسج/ الاميبا المتغايرة Entamoeba histolytica/Entamoeba dispar في عينات البراز للمرضى با ستعمال تقنية PCR == Detection of Entamoeba Histolytic /Entamoeba Dispar In Stool Specimens By Using PCR Technique

Author name: بثينة عبد الحمزة حسون الزبيدي
Supervisor name: اخلاص مشرف عيدان
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: تسلط الدراسة الحالية الضوء على تشخيص وتحديد نسبة الاصابة بطفيلي الاميبا المرضية المتمثلة بالاميبا الحالة للنسج Entamoeba histolytica وتمييزها عن الاميبا غير المرضية المتمثلة بالاميبا المتغايرة Entamoeba dispar اللتين تتمايزان بشكلهما المتماثل في كلا الطور | This study sheds light on the determination and diagnose of the percentage of infection of like Entamoeba histolytica and differentiate it from the non pathogenic Entamoeba dispar that were morphologically identical in both cysts and trophozoite phase in two different groups : the first group includes 50 stool samples of patients suffering from diarrhea and abdominal pain (symptomatic group) while the second group includes 95 stool samples of patients not suffering from diarrhea and abdominal pain (Asymptomatic group).In addition to that, 20 stool samples were collected from healthy individuals as a control these samples were collected patients of AL - Zafaranyia General Hospital and AL - Alwyia Childhood Teaching Hospital in Baghdad from July, 2011 to May, 2012.These samples belong to different ages (1 year - < 50 years) of different sexes (males and females). Entamoeba samples were diagnosed by using two methods : the first one is the wet mount as a General Stool Examination(GSE) after macroscopic examination for sample and classifying them according to their consistency (solid, semisolid and liquid) and color (brown and yellow) and presence of blood and mucous in addition to diagnosing them microscopically to detect the presence of pus cells and both phases trophozoite and cyst for Entamoeba histolytica.While the second method that was depended in diagnosis is duplex polymerase chain reaction (dPCR) by using two pairs of genes for detection of their amplification in DNA sample isolated from stool. The first pair is cystein proteinase which represents one of virulent factors in Entamoeba histolytica parasite as a tissue invader. The second pair encodes the surface antigen present on the surfaces of both parasites E. histolytica and E. dispar. It is represented by Actin gene (Act). The results of the wet mount showed that in percentage of infection of both species (E.histolytica/E.dispar) the (54%) of patient symptom and (34.37%) patient a symptom.While results of the specificity and sensitivity showed that in positive sample wet mount negative PCR while negative sample wet mount showed that positive PCR and higher percentage of infection the Entamoeba dispar. While the results of the dPCR showed that in spite of the presence of both species Entamoeba pathogenic (Entamoeba histolytica) and non pathogenic Entamoeba (Entamoeba dispar) , the percentage of infection of Entamoeba histolytica was lower than that of Entamoeba dispar. This was clear by the detection of amplification the Reaction producets (dPCR) by using both genes : Actin &Ehcp5 where only 8 samples were detected and they were found to be positive amplification Ehcp5 and 31 samples were positive amplification in both groups of patients (symptomatic and a symptomatic). The percentage of infection in females was higher than that in males for all ages when using both tests (wet mount & PCR). More over the patients with less than (10) years old had higher percentage of infection in both sexes.In conclusion, it should not be depended on direct wet mount technique for the identification of infected parasite Entamoeba histolytica and differentiating it from Entamoeba dispar because of their high homology. That the molecular examination is the only method that is able to determine the kind of infection with parasite Entamoeba

تاثير مستخلص الثوم والرمان على الجرذان المصابة تجريبيا بطفيلي Cryptosporidium parvum ومقارنتها مع عقار Metranidazole == Effect Extract Allium Sativum And Punica Grantium On White Rat Expermental Infection Cryptosporidium Parvum And Comper With Metranidazole

Author name: سرى رزاق خضير عبادة
Supervisor name: نجم عبد الواحد عبد الخضر الجدوع
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: جمعت عينات البراز من الاطفال في مستشفى النسائية والاطفال كذلك جمعت من العجول المصابة حيث اجري فحص العينات بصبغة الزيل نلسن ثم عزلت ونقيت الاكياس بطريقة التطويف بمحلول شيذر السكري وجرعت 2103xكيس بيض لكل جرذ بانبوبة التجريع الفموي وسجلت المدة قبل البائنة من | Collects stool samples from children in the women's and pediatric hospital. also collects all those from infected calves, where he conducts testing samples dye Zail Nelson then isolated and scrubbed bags manner floation solution Shidhir diabetes and gived rat 2 x103 bag eggs per rat tube dosage oral and recorded for the period before dowry of 3 - 5 days as recorded wounding 100%. The result is the appointment of the lethal dose of moderation LD50 of aqueous extracts of the garlic, peel the pomegranate after giving the rats doses graded by the mouth and the results indicate that the dose 7500 mg / kg of body weight is the lethal dose of aqueous extracts of garlic bulbs and dose limits of 5250 mg / kg is the lethal dose to peel pomegranate then tretment using cold aqueous extract to plant garlic. Allium satvuim L. peel and pomegranate Punica granantum L and drug Metronidazole (Flagyl) concentration of 250, 500, 750 mg / kg, the results shows the effectiveness of the current study, the aqueous extracts in the treatment of the parasites it turns out that the time required to kill the parasite suit inversely with the increasing concentration of extracts also show the efficiency of the aqueous extract of garlic bulbs and metronidazole concentration of 750 mg / kg in the treatment of 100% after 13 days of treatment with stops put bags of egg either concentration of 500 mg / kg of aqueous extracts garlic bulbs and metronidazole has shown the efficiency of treatment of by 100 % after 15 days of treatment showed, while the aqueous extract of pomegranate peel concentration 750 mg/kg therapeutic efficiency of 98.41% after 15 days of treatment, while the control group continued to launch until after the egg sacks (15) of treatment. The study shows histopathological section on the small intestine (duodenam, ileum, jejunum) Rats infection parasite get pathological changes represented by the crash of the villi of the small intestine and change epithelium from the vertical to the cube and the adhesion of some of the villi and gatherings of inflammatory cells in layer plate fitted, as well as for necrosis and desquamation of the cells lining the villi also destroyed gland and appeared egg sacks in the intestine result use 2x103 frome egg sacks also appeared effective abstract aqueous garlic in the intestinal tissue to restore its natural form after 15 day use concentration 750 mg/kg of aqueous extract of garlic bulbs and metronidazole while have taken normal sat to tissue treaement with aqueous pomegranate peel long than 15 day. test the effectiveness of aqueous extracts cold of garlic bulbs. Alluium sativum L. and peel pomegranate Punica granantum L in treatment parasite spores Cryptosporidum parvum and compared with the treatment of Metronidazole (Flagyl) in laboratory rats. Conducted on plant extracts cold of garlic and pomagranite the chemical tests have shown the presence of active substances of both alkaloids, carbohydrates, Flavonides, Alglycosidat, Alsaponyat, Alresins, phenolies

دراسة وبائية وسريرية لمرض الجيارديا Giardiasis في اطفال مدينة الشرقاط وضواحيها == Parasitic Study of Giardiasise Among Children In Sharqat City

Author name: علي حمد طلال موسى الجبوري
Supervisor name: حسين ساهر اسود العبيدي | عبد الله حسين عبد الله الجبوري
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت هذه الدراسة لبيان تاثير طفيلي الجيارديا المعوي على صحة الاطفال في قضاء الشرقاط لـ(250) طفل، تم اختيارهم بصورة عشوائية، اذ تراوحت اعمارهم من شهر - 15 سنة وللمدة ما بين شهر تشرين الاول 2006 لغاية شهر تشرين الاول 2007، تم فحص عينات الغائط من كل طفل بال

دراسة تشخيصية وجزيئية لطفيلي الجيارديا المعوية Giardia intestinalis لدى الاطفال المصابين بالاسهال في محافظة القادسية == Diagnostic And Molecular Study of Giardia Intestinalis In Children Infected With Diarrhea In Al - Qadisiya Province

Author name: لبنى عبد القادر خنياب الابراهيمي
Supervisor name: هادي مدلول حمزة الميالي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: تضمنت الدراسة الحالية فحص 926 عينة غائط للاطفال المصابين بالاسهال والمراجعين لمستشفى الديوانية التعليمي ومستشفى الولادة والاطفال، وبعض المراكز الصحية والمختبرات الاهلية في محافظة القادسية خلال المدة ما بين 1/11/2012 الى 30/6/2013 وللفئات العمرية من ?1 - 1 | The current study included examination of 926 stool samples of children with diarrhea who attended to didactic hospital in Al - Diwaniya. The maternity hospital, some health centers and competent laboratories in Al - Qadisiya province during the period from 1/11/2012 to 30/6/2013 and for age range of ? 1 - 12 year exclusively. The current study showed the rate of total infection of Giardia parasite was 5.61% (52 of 926 samples). They were examined by direct wet smear method and floatation method by using light microscope to detect the trophozoite and cyst phases of parasite. It was found in numerous stool samples. The results of samples showed that the infection in males was higher than in females 6.12% and 5.11 % respectively. The age range 2 - 4 years recorded highest incidence of infection and the age range 8 - 10 years recorded the lowest incidence of infection 13.87% and 1.52 % respectively. Also it was recorded higher infection rate in June and the lower infection rate in January 10.37% and 1.92% respectively. It was recorded higher infection rate among the rural population in comparing with urban population 7.07% and 4.45 % respectively. statistical analysis results show a significant differences at(P?0.05). In the present study, Real - Time polymerase chain reaction (PCR) was used to detect Giardia parasite infection in microscope positive examined samples where it had record total Infection rate of 73.07% (38 of 52). Statistical analysis of survey results after depending on PCR technique showed significant differences in infection among the age range. The age range 2 - 4 years had recorded the higher infection rate 83.33% while there are no significant differences of infection according sex.It reached in males to 75% in comparing with females 70.83 %, also it did not show significant differences in infection rate according to living areas.It had reached in rural areas to 75.86 % in comparing with urban areas 69.56%. In addition, polymerase chain reaction technique was used (PCR) and restriction fragment length polymorphism (RFLP - PCR) to determine genotypes of Giardia intestinalis parasite for 20 case from diarrhea positive cases of children in Al - Qadisiya province, by amplifying gene (gdh) glutamate dehydrogenase gene by using specialists primer. They are GDHiF and GDHiR It got the amplification results successfully in all stool samples of 20 and positive examined Real - Time PCR were it had determined the existence of gdh gene of molecular weight of 432bp in all those samples. The study showed that 7 of the previous samples belong to genotype A (35%) and 13 samples belong to genotype B (65%).It showed that all samples of genotype A belong to secondary genotype AII in rate 100%, while samples of genotype B belong to two secondary genotypes, they are BIII (61.53%) and BIV (38.46%). The study showed the appearance of genotype A in the age range under six years, while genotype B in all age range approximately. The distribution of genotypes according to sex and housing it had record both genotypes appeared in both sex with different rate genotype B was more appearance than genotype A in males than females. According to the nature of housing secondary genotype AII had appeared in rate 70% in urban areas, while genotype B with its secondary different genotypes in both areas urban and rural, but with higher rate in rural areas (100 % and 30 % respectively). We had concluded from the current study that there is a close relationship between infection, the sex, age range, nature of settlment and monthly distribution.There are two basic geno types in province, they are A and B, for each of them there aresecondary genotypes AII, BIII, BIV.

دراسة بيئية وتصنيفية للقواقع المائية في مدينة النجف الاشرف وبعض المؤشرات حول حكة السباحين == Taxonomic And Ecological Study of Aquatic Snails In The Al - Najaf Al - Ashraf City And Some Pointers About Swimmer’s Itch

Author name: اسراء عبيد حسين الشبلي
Supervisor name: جاسم حميد رحمة الخزاعي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: اجريت الدراسة الحالية في محافظة النجف الاشرف تم جمع عينات القوقع من ثلاث محطات ( (منخفض بحر النجف وقضاء المشخاب والعباسية)) للفترة من 1 - 2 - 2013 لغاية 30 - 10 - 2013 وفحصت العينات في مختبر اللافقريات التابع لكلية التربية للبنات لغرض تشخيص الاطوار اليرقي | This study was conducted in Al - Najaf Al - Ashraf province and the samples were collected from Bahar Al - Najaf, Al - Mashkhab and Al - Abbasia, during the period 1/2/2013 untill 30/10/. The samples were in the laboratory of invertebrates in College of Education for Girls to diagnos the larva stages of Trematodes which parasitize on the aquatic snails with some of the indicators and observations about the causes of Swimmer’s itch disease. The lowest water temperature in AL - Abbasia water was 13?c in February while the highest temberature was recorded in Bahar Al - Najaf 30.4?c in August. The PH ranged between (7 - 8.5).The Electrical Conductivity recorded the lowest rate in February in Al - Abbasia 1.16 Microsimnez / cm and the highest rate in March in Bahar Al - Najaf 5.97 Microsimnez / cm and the salinity concentration was lowest the in February in Al - Abbasia 0.58 ppt, while the highest rate was recorded in March in the Bahar Al - Najaf (2.98) ppt and Turbidity was the lowest rate in Al - Mishkhab in October 2.8 Unit Turbidity nephlometric and the highest rate was in the AL - Abbasia in July (4.42) units Turbidity nephlometric while The Dissolved Oxygen recorded the lowest level in the Al - Abbasia 2 mg / L in August and the highest level in April in Al - Mishkhab 8.5 mg / L. The study showed the presence of five types of snails : Belamia bengalensis, Lymnae auricularia, Physa acuta, Melanopsis nodosa and Melanoides tuberculata. therefore, there are five species of snails in Bahar Al - Najaf while P.acuta was not recorded in Al - Abbasia and Al - Mashkhab in addition, the numbers of the snails varied according to the time, so the B.bengalensis and M.nodosa increased in June, July and August while L.auricularia and P.acuta and M.tuberculata snails increased during spring season. All snails that have been diagnosed were infected with (Miracidium, Sporocyst, Ridia and five types of Cercaria) stages.Also, the highest infection in the M.tuberculata was 36.3%, B.bengalensis 31.3%, M.nodosa 25.4%, L.auricularia 24 % while the snail P.acuta recorded lower infection rate at13.7%. The Results of the questionnaire revaled that the males infection in Swimmer’s itch was 76% higher than females.The highest infection was among (10 - 20 years) it reached 36%, and 39% in fishmen and aquatic birds which is the highest rate compared with others. The highest rate of infection was recorded in August (42 %) a large number of the patients exhibited symptoms of the disease after coming out of the water and dehydration reached 52%.The results showed that 75% of patients showed Symptoms of the Swimmer’s itch after (3 - 7days), also the hands and legs were more susceptible to infection 62 %, and the highest percentage of the S.dermatitis 86% wdetected in person swimming in Shallow water

علاقة مستوى الزنك والنحاس ببعض المعايير المناعية في الاصابة بداء المقوسات في النساء في محافظة كركوك مع محاولة علاجية في الفئران المختبرية == Relation of Zinc And Copper Levels With Some Immunological Markers In Women With Toxoplamosis In Kirkuk Governorate With Trial To Treatment In Mice

Author name: عبير عباس علي
Supervisor name: حسين ساهر اسود العبيدي | شهاب احمد محمد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تم دراسة 100امراة مصابة بداء المقوسات Toxoplasmosis الذي يسببه الطفيلي المقوسة الكوندية Toxoplasma gondii للفترة من اذار 2007 ولغاية نيسان 2008 راجعن العيادة الاستشارية التابعة لمستشفى كركوك العام في مدينة كركوك. اظهرت نتائج الدراسة الحالية ان 56 % من الن | A total of 100 patients suffering from Toxoplasmosis which is infected by the parasite Toxoplasma gondii have been dealt with as subject of the sample during the period from March 2007 to April 2008 at the Consultant Clinic of Kirkuk General Hospital. The results of the present study showed that 56% of the subjects were pregnant and 44%were not. The highest percentage of the infected subjects was of 26 - 30 years old as 30.37% among the pregnant women and 36.35% among the non - pregnant women of the age mentioned above. The highest percentage 37.5% of abortion of the first period of pregnancy was in pregnant women, and 45.53% in non - pregnant women. The results of the study also showed more cases of infected women among those who bred animals in their living places as 69%. It is also found that the highest percentage of the infected women 64% were of those who use the running water for drinking. Moreover, it is noticed that there is an increase in the percentage of the infected women among the uneducated women 38%. The study showed that the percentage of the infection is proportional to residence 63%, the infection increase among women living in rural areas. The percentage of T - lymphocytes in peripheral blood of patients were also measured through measuring the percentage of the CD4+ and CD8+cells by using fluorescent microscope.The results showed a significant decrease (p<0.05) in this type of cells.The rate of percentage of CD4+ of the patient's were 31.704±0.116 while the rate of CD4+ in the peripheral blood of the control group was 62.260±0.237.The rate of percentages of CD8+ 19.382±0.080, whereas it was 31.680±0.307 of the control group.The rates of the percentages of CD4+ to CD8+ were also measured, the results showed a significant decrease (p<0.05) in the rate of the percentage in the patient's group 1.642 while it was 1.968 in the control group. The statistic analysis showed significant differences between these tow groups (p<0.05). The intensity of Zinc and Copper has been measured in the serum of the infected women via use of Atomic absorption instrument, a significant decrease (p <0.05) appeared in the rate of Zinc intensity in the serum on the infected women as compared with the control group.The rate of the intensity in patient's group was 4.65% ± 2.628µmol /l while in the control group it was 15.353± 1.640 µmol /l.A significant increase (p<0.05)was also noticed in the intensity of Copper in the patient's group (28.589±4.335 µmol /l)whereas it was 12.854±1.501 µmol /l in the control group. The index of the specific antibodies IgM and IgG related to Toxoplasma gondii was measured by ELISA method, a significant increase (p<0.05) was observed in the value of IgM and IgG in the patient's group as compared with the control group. The rate of index for IgM in the patient's group was 1.354±0.05 while it was 0.163±0.05 in the control group. The rate of value of IgG in the patient's group was 1.163±0.074 whereas it was 0.358±0.033 in the control group. In the present study, Toxoplasma gondii was isolated and diagnosed from human as first attempt, and then the infection was created in laboratory mice as an essential step for growing the parasite. The second step was studying the effect of some selected drugs on the experimental treatment for disease of toxoplasmosis created in the mice. The drugs are : Pyrimethamin, Clarithromycin, Spiramycin, Ivermectin.The results of the study showed the effects of these drugs on the tissue cyst formed in the brain and lungs. The number of tissue cysts in the brain of the mouse treated with pyrimethamin was 14.8 ±1.067 after 28 days. Significant differences appeared when this result compared with the control group in which it was 27.4±1.029. The number of tissue cysts formed in lung, tissue in the mouse treated with the same drug after the same period of the time was 6.2±0.583.This showed a significant differences as compared with the control group in which it was 10.2±0.583. No significant differences appeared between both groups as for the treatment by Clarithromycin antibiotic as far as the number of the tissue cysts formed in the brain after 28 days is concerned. The number was 23.8 ±0.86 as compared with the control group 38.6±1.029.The same compared is true of the lungs, tissue. The number of the tissue cysts was 23.8±0.86 as compared with the control group 28.4±1.805. Concerning the antibiotic Spiramycin, the results showed the effectiveness of it in decreasing the tissue cysts formed in the brain.The significant differences were (p<0.05), the rate of the cysts was 9.6±0.6 as compared with the control group in which it was 22.8±1.067.The same effectiveness appeared on the lungs, tissue as the rate of tissue cysts after 28 days of treatment was 4.6±0.509 as compared with the control group in which was 17±1.14 and the significant differences were (p=0.000).The Ivermectin drug showed a great effectiveness in minimizing the number of the tissue cysts formed in the brain and the lungs. The numbers of the tissue cysts formed after 28 days were 3.2±0.374 and 1±0.316 respectively; significant differences are observed when these numbers are compared with the numbers of the control group which were 22±1.843 and 19.4±1.435. Thus, the use of Ivermectin drug is considered as a pioneer and fruitful study since this drug has not been used for the treatment of Toxoplasmosis.

دراسة بعض مظاهر امراضية الخمج بالمتحولة النسيجية بين الاشخاص في مدينة سامراء == A Stud Y of Some Aspects of Entamoeba Histolytica Pathogenicity Among Peoples In Samarra

Author name: صلاح عبد حسن السامرائي
Supervisor name: ابراهيم شعبان داوود | حسين ساهر اسود
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: شملت هذه الدراسة فحص (540) عينة براز من المصابين بالاسهال والمراجعين لمستشفى سامراء العام وذلك خلال الفترة الممتدة بين 1/10/2007الى 1/6/2008 فحصت العينات بطريقة المسحة المباشرة المزدوجة للكشف عن الاطوار الخضرية والمتكيسة لطفيلي اميبا الزحار. واظهرت النتائ | The current study dealt with examination of (540) stool samples for patients with diarrhea who visited the General Hospital of Samarra during 1/10/2007 - 1/6/2008. The samples were examined by double wet direct smear method to identify the trophozoite and cyst stages of Entamoeba histolytica. The results showed that (112) were infected with amoebic dysentery. The total rate was (20.74 %). (262) are males with a rate of infection (19.84 %), whereas, the number of females was (278) with an infection rate (21.58 %). The study showed an increase rate of infection among the rural population, it was (25.71 %), whereas the urban population rate was (16.6 %). The study covered all ages beginning from less than a year to 75 years old. A highest rate of infection was recorded by (10 - 19) years old, it was (29.4%). The study also, found out that a highest rate of infection was during school months, particularly during November (27.5 %). Examinations of complete blood picture which included Hemoglobin concentration (Hb), packed cells volume (PCV), white blood cells count (WBCc), and erythrocyte sedimentation rate (ESR) were done on all patients infected with amoebic dysentery. The results were compared with those of healthy ones (Control Group). The results showed significant differences between the two groups. According to the significant one (P < 0.05), it recorded a decrease (16.9%) in the (PCV) and (25.1%) in the (Hb) and an increase (241.8%) in the (ESR) and (51.5%) in the (WBCc) for those ones infected with amoebic dysentery if compared with the control group. Clinical biochemistry examinations were performed, on blood sugar (Bs), electrolytes such as Sodium (Na+), Potassium (K+), Calcium (Ca++), Magnesium (Mg++), liver functions tests : total serum bilirubine(TSB), tranceaminase enzyme(GOT, GPT), alkaline phosphetase (AlP), total protein (TP) and albumin (Alb). The results showed that there are significant differences between persons infected with amoebic dysentery and the healthy ones according to the significant level (P < 0.05) in these examinations. The study showed a decrease(21.6%) in the (Bs) mean concentration, (16.8%) in the(K+) mean concentration, (5.8%) in the(Ca++) mean concentration, (4.5%)in the (Mg++) mean concentration, (10.5 %) in the (Na+) mean concentration , (17.2%) in the (TP) mean concentration and(12.2%) in the (Alb) mean concentration and increase (83.7%) in the (Alp) mean concentration, (52.1%) in the(TSB) mean concentration, (81.1%)in the (GPT) mean concentration, (59.9%)in the (GOT) mean concentration and(22.8%) in the (globulin) mean concentration in the persons infected with amoebic dysentery compared with the mean concentrations in healthy ones control group.

تحديد تجمعات Giardia lamblia ومعقد Entamoeba histolytica/Entamoeba dispar بالتشخيص الجزيئي (PCR) للمصابين في تكريت == Determination of Giardia Lamblia Assemblages And Entamoeba Histolytica/Entamoeba Dispar Complex By Molecular Diagnosis (Pcr) In Patient In Tikrit

Author name: انتصار غانم عبد الوهاب الصميدعي
Supervisor name: شهاب احمد محمد الجبوري | عقيل حسين العاصي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت هذه الدراسة للمدة من كانون الثاني 2011 ولغاية كانون الاول 2011 للمرضى الراقدين والمراجعين لمستشفى تكريت التعليمي وبعض المختبرات الخاصة في مركز المدينة وضواحيها والذين يعانون اعراض مرضية مختلفه ولفئات عمرية مختلفة ترواحت بين اقل من سنة الى 61 سنة، ح | This study was conducted for the period started from January 2011 until December 2011 on inpatients and outpatients of Tikrit educational hospital and some private laboratories at Tikrit city and surrounded suburbs. Symptoms of different diseases among various age groups varies between less than one year to 61 years. The tests used the (ELISA & PCR) and the analysis of the PCR - RFLP to discriminate the infection by assemblage of Giardia lamblia and differentiate between pathogenic amoeba Entamoeba histolytica and nonpathogenic Entamoeba dispar compared with microscopical examination. The result of present study showed that (75) person who represent (37.5%) were infected by Giardia lamblia and (75) person who represent (37.5%) were infected by E.histolytica/E.dispare complex, out of (200) stool samples which have been tested by microscopical examination. Epidemiological study showed no significant difference among the sex for the two species parasites whereas a high percentage for infection recorded in group at age (1? - 10) year for Giardia lamblia and E.histolytica / E.dispar complex reach to (60%, 36%) respectively. Regarding the place of living Giardia lamblia in rural area showed infection rate of (19.5 %), while infection rate by E.histolytica/E.dispar complex reaches (22.5%) in urban area. Statistical analysis shows a high difference of significance according the source of water supply. Tap water showed high incidence for initiating infection which reaches to (93.3%) by G.lamblia and (90%) for E.histolytica/E.dispar complex. For the infection rates and their relationship to the educational level, the results show that the highest rates of infection recorded in non - educated (52%) and (73.3%) of Giardia lamblia and E.histolytica / E.dispar complex, respectively. Depending on the sample texture, the result show that oily diarrhea is more correlate with infection reaches to (33.3%) of Giardia lamblia and the watery diarrhea form 40% by E.histolytica / E.dispar complex, while simultaneous occurrence of both symptom (colic and loss of appetite) are the most symptom synchronized with infection records (44%) for Giardia lamblia and (36%) for E.histolytica / E.dispar complex. The percentage of infection by Giardia Lamblia in this study depends on the test results of the ELISA was (20%), while the percentage of infection E.histolytica/E.dispar complex was (6.7%). Testing has shown sensitivity compared to microscopic examination reaches to (22.6%) and (8%) for both parasite respectively, while the test showed a high specificity which reaches the rate (93.3%) and (100%) for both parasites respectively. Result of PCR showed a percentage of infection by E.dispar about (32%) (64 sample) from the total samples, whereas it does register any cases infection by E.histolytica. By counting the results of the PCR for the three genes (ssu rRNA + TPIA + GDH) they show a total infection by Giardia lamblia which was (10.5%) (21 samples). This study recorded percentage of infection Giardia lamblia depending on the result of the three Genes separately amounted to (4%), (5%), (3%) for (ssu rRNA, TPIA, GDH) respectively. According to the result of the analysis PCR - RFLP for GDH gene by using the enzyme NIaIV, it did not record any infection by Giardia lamblia (assemblage A), whereas (6 samples) recorded (100) by Giardia lamblia (assemblage B), by using the enzyme Rsal to determine the secondary assemblage shows that the total (6 samples) infected there are 5 samples carrying infection by two type of secondary assemblage returning to the assemblage B (assemblage BIII +BIV) and only one sample infected with (assemblage BIV). To determine the assemblage and secondary assemblage for the parasite result compared with amplification of TPIA gene with lysis by enzyme for the resulting amplification for GDH gene, resulting a ratio of (14.3%) for a sample infection by both assemblage (A+B) and secondary assemblage (BIII+BIV) together, and a ratio of (57.14%) infection by assemblage A only and (21.4%) infection by secondary assemblage (BIII+BIV) together and (7.14%) infection by BIV only.

دراسة بعض التاثيرات المرضية للدودة الدبوسية Enterobius vermicularis لدى الاطفال في محافظة النجف == The Study of Some Pathologaical Effects of Pin Worm Enterobius Vermicularis Among The Children In Al - Najaf Province

Author name: ساهرة عايد عبد الصاحب الموسوي
Supervisor name: هيثم محمد حمادي العوادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: اجريت الدراسة الحالية للمدة من شهر تشرين الثاني 2011 الى شهر تموز 2012, لمعرفة تاثير الدودة الدبوسية Enterobius vermicularis في بعض المعايير الدموية والكيموحيوية والسريرية لدى الاطفال المصابين بالدودة (12 - 4سنة). استخدمت طريقة الشريط اللاصق الاسكتلندي في | The present study was conducted during the period from November 2011 to July 2012, to evaluat the effect of the pin worm Enterobius vermicularis on the Hematological, biochemical and chlinical parameters in infected children (4 - 12 year).This study included 300 patient from both sexes and different ages. The Scotch Cellulose Tape Technique used for the examination of pin worm in children, attended Al - Sader Educational Hospital, Al - Zahraa hospitals and central laboratory in Al - Najaf Al - Ashraff city. The results of the sudy showed a significant decrease was noticed in Hb and PVC in infected person compared with the control group While there was a significant increase in each of the total number of white blood cells and Eosinophils and Neutrophile and a decrease in Lymphocyte, and it did not show any significant difference in the Basophilis and Monocyte in children infected with pin worm compared with the control group.The results of the study showed a significant increase in the levels of Nitric Oxide, Malondialdehyde, it also showed a significant decrease in the levels of Gluthanione in the infected group compared with the control group. The concentration of Copper, Zinc, Magnesium and Vitamine B12 were low in children infected with pin worm compared with the control group. The high incidence rate of infection accompined with patient suffering from pruritus ani and nocturnal enuresis. The age group (10 - 12) year represent the highest group infected with the worm.The education level influence the distribution of these worm among un educated families, and ratio of infection increase in male and female in families which contain more than (10) members with significant difference P<0.05 and this ratio decreased in children in families which contain between 3 - 4 members.This study did not show any significance defferent in infection among the ratio of infection between male and female under P<0.05.

التحري عن طفيلي المقوسة الكوندية Toxoplasma gondii في الطيور الداجنة في منطقة الفرات الاوسط والقطط في محافظة الديوانية باستخدام التقنيات المصلية والجزيئية == Detection of Toxoplasma Gondii In Domestic Avian In Middle Euphrates Region And Cats In Al - Diwania Province By Serological And Molecular Techniques

Author name: خديجة عبيس حمود الخالدي
Supervisor name: خيري عبد الله داود العكيلي | هادي مدلول الميالي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: تضمنت الدراسة الحالية الكشف عن طفيلي المقوسة الكوندية Toxoplasma gondiiفي اربع انواع من الطيور ضمت الدجاج المحلي Gallus gallus domesticus والديك الروميMeleagri galbpavol والاوز الاربد Anser anser والبط المحلي Anas platyrhychos domesticus في منطقة الفرات ا | The current study was conducted to detect of Toxoplasma gondii in five species of avian including, Gallus gallus domesticus, Meleagris galbpavol, Anser anser, Anasplatyrhychos domesticus in middle Euphrates provinces of Iraq, also in cats in AL - Diwania province, during 2011 - 2013 by using serological tests (Latex, Rapid test cassette) and molecular test (conventional - PCR and Nested - PCR) as confirmative tests to detect of B1gene which was specific gene of T.gondii in blood and Tissue samples of different organs such as Liver, Heart, Brain, Eye, Lung, Pectoral muscles, Small intestine, Spleen, and Leg muscles of avian and cats which were seropositive cases of Rapid test cassette, also the study was included the detection Oocysts of T.gondii in soil samples of ten cities from Al - Diwania province by using sedimentation and floatation methods as primarily diagnosis, also using the polymerase chain reaction to detect of B1gene in these samples. A total of 320 Avian collected, include 80 samples of each species (20 samples of chicken, Turkey, geese, and Ducks) of each province of middle Euphrates (AL - Diwania, Babylon, AL - Najaf and Karbala), in addition to 10 samples of cats and 100 samples of soil from the center of AL - Diwania province, samples of avian and cats were involved to serological test such as LAT, the results showed there were 96 positive samples in percentage %30, include, 31(%38.75) of chicken, 28 (%35) of turkey, 22 (%27.5) of geese, 15 (%18.75) of ducks and 5 (%50) of cats, the higher Percentage (%23.95) of infection with T. gondii in avian was in titer 1/160 and the lower percentage (%3.73) in titer 1/640, Whereas in cats, the higher percentage in titer 1/20 and lower percentage in titers 1/40, 1/80, 1/160 which reaches %20. The results of Rapid test cassette were showed there were 60 out of 320 samples in percentage %18.75, include 24 (%30) of chicken, 19 in (%23.75) of turkey, 10(%12.5) of geese, 7(% 8.75) of duck and 3(%30) of cats. Also the results of Rapid test cassette were showed the higher percentage of types of antibodies in the serum of avian was %36.66 of IgG +IgM together and the lower percentage was %28.33 of IgM, whereas in cats, the higher percentage was %66.66 of IgG and the lower percentage was %33.34 of IgG +IgM together. To confirm the infection of avian by T.gondii, Polymerase Chain Reaction was used to detect the B1 gene (399 bp) in 320 blood samples of avian and 10 samples of cats, the results were showed there were 34 samples (%10.62) of avian gave positive result, include, 14 (%17.5) of chicken, 12 (%15) of Turkey, 8 (%10) of geese, whereas the results were showed the B1 gene was not present in the blood samples of ducks and cats. The results of Latex, Rapid test cassette and polymerase Chain Reaction were showed, the higher percentage was recorded in chicken % 17.5, %30, %35, whereas the lower percentages were %18.75, %8.75, %0 in D ucks by using the three tests, respectively. According to the results of Rapid test cassette (IgG, IgM +IgG), the results of polymerase Chain Reaction to detect the B1 gene in 16 samples of avian (4 samples of each species) out of 39 samples and 3 samples of cats, showed there were 9 samples (%56.25), include 4 (%100) of chickens, 3(%75) of turkey, 1(%25) of geese, and 2(%66.66) of cats and the higher percentage of B1gene (399bp) in livers of avian was %55.55, %22.22 in pectoral muscles then heart, spleen and small intestine in percentage %11.11 for each one of them, whereas in cats the percentage were % 66.66 in liver while in brain and kidney the percentage was %33.34 of each one of them.. The results of Nested Polymerase Chain Reaction for amplification the gene B1 in 12 blood samples (4 samples of each species of birds except ducks) was positive for conventional - PCR, Showed the amplification occurs in 4 samples in percentage % 33.33 during the first round of reaction and 3 samples in percentage %75 in the second round, in 10 samples of tissues, the amplification occurred in 6 samples in percentage %60 during the first round and in 4 samples in percentage % 66.66 during the second round of reaction. Also the results were showed the percentage of T.gondii antibodies in serum of four types of avian (except pigeons) according to the provinces were %28.75 in AL - Diwania, %40 (Higher percentage) in Babylon, %12.5(Low percentage) in AL - Najaf and %38.75 in Karbala by latex test, whereas the percentages %26.25 (higher percentage), %10 (lower percentage), %12.25, %17.5 in each of Karbala, AL - Najaf, Babylon, AL - Diwania province respectively by Rapid test cassette, and by using polymerase chain reaction, the percentages were %16. 25 (Higher percentage), %5(Lower percentage), %6.25, %15 in AL - Diwania, Babylon, AL - Najaf and Karbala province, respectively. Also the results showed, the percentage of infection in AL - Diwania province the samples of rural areas were higher (%37.5, %22.5, %17.5) whereas the percentages were lower (%20, %12.5, %12.5) in urban areas by using latex, rapid test cassette, and polymerase chain reaction, respectively. The results of comparison between two serological tests and Polymerase Chain Reaction, showed the percentages of infection were % 10.62 (34), %18.75 (60), %30 (96) by using Polymerase chain reaction, Rapid test cassette, latex test, respectively, also the sensitivity and specificity of latex test were %35, %72 whereas the sensitivity and specificity of Rapid test cassette were %35, %88, respectively in compared with standard Polymerase Chain Reaction. In the soil samples, the percentage of presence of Oocysts of T.gondii was %10 by using floatation method and %4 by conventional Polymerase Chain Reaction. The study of grossly and microscopy pathological changes in avian and cats which infected with T. gondii, was showed there were lesions on each of liver, heart, pectoral muscles, small intestine, brain and kidney.

دراسة تشخيصية وتصنيفية لطفيليات الديك الرومي Meleagris gallopavo في محافظة القادسية == Diagnostic And Taxoaomical Study of Turkey (Meleagris Gallopavo) Parasites In Al - Qadisiya Province

Author name: هند عبد الزهرة عبد الكاظم الشباني
Supervisor name: هادي مدلول حمزة الميالي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: خلال المدة ما بين بداية شهر ايلول 2014 ونهاية ايار 2015 جمع وفحص 50 طيرا من الديك الرومي Meleagris gallopavo من مناطق مختلفة من محافظة القادسية في مختبرات الكلية بهدف عزل الطفيليات الخارجية والداخلية المتطفلة على هذه الطيور وتشخيصها فضلا عن دراسة نسبة الا | The current study carried out during the period between the first of the September 2014 end of May 2015.fifty birds of domestic turkey Meleagris gallopavo was collected from different areas of Al - Diwaniya city markets, to isolating and identification the external and internal parasites parasitized on these birds as well as study the incidence and intensity and the most important pathological and histological changes resulting from external and intestinal infections, Six species of external parasites was isolated included four species of biting lice Gonoiocotes gallina, Goniodes gigas, Menacanthus straminus and Oxylipeurus sp. The infection rate and severity of 10%, 6.81.4%, 4.72.2, %, 54.2%, 0.09, respectively, and Two species of Ticks are Argus persicus and Haemophysalis sp. Nymph. and the rate of infection was 2%, 0.36; 2%, 0.24 : respectively. The results showed that the triple infection of lice are the most common, (63.63%.) While internal parasites that 9 species of intestinal parasitic worms in the digestive system included two species of Protozoa Eimeria sp. and Histomonas meleagridis The infection rate and intensity was14%, 4.20 0.2%, 0.14, respectively, Four species of tape worms Railletina cesticillus, Railletina tetragona. Choanoyania infundibulum and Hymenolepis sp.The intensity of infaction was 2%, 100.2% 53.284, 4, 00, 2% 2%, 0.42; respectively, Three species of nematodes Ascardia gallinarum, Capillaria sp. and Heterakis gallinarum and severity of infection was 21.73%, 1.2; 2% 0.100; 17.79%, 1.25; respectively. The study showed the presence of internal parasitic worms sites in the gastrointestinal tract it was observed that most of tapeworms parasitize in the small intestine and rates higher than what is found in other parts of the gastrointestinal tract, and the protozoa are found in the liver and small intestine. The nematodes are also found in various parts of the gastrointestinal tract, including both of the small intestine and the large intestine and esophagus. The species Haemophysalis sp. and Oxylipeurus sp. and helminths Raillietina cesticillus, Raillietina tetragona, Choanotania infundibulum, Hymenolepis sp. Ascardia gallinarum, Capillaria sp.Heterakis gallinarum. and Eimeria sp. were the first recording in turkeys in Iraq. Also the current study showed a many of gross and microspcopic pathological changes caused by lice in infected birds, such as lethargy, laziness and sagging wings and nervous tension and break the feathers and hair loss, as well as change the color of the skin also appeared many of the macroscopic and microscopic changes in the gastrointestinal tract as a result of, intestinal worm included changes macroscopic obstruction and hemorrhagic bruises and blood infiltration The microscopic changes have included the infiltration of inflammatory cells Nicrosis and abstraction of cells lining the of these channels and the proliferation of Kubffer cells and expansion in sinosoids and atrophy of the villi tissue.

تشخيص طفيلي المشعرات المهبلية بتقنية تفاعل البلمرة المتسلسل RT - PCR في محافظة واسط - العراق == Diagnosis of Trichomonas Vaginalis Using Real - Time Pcr Technique In Wasit Province - Iraq

Author name: رسل واسط كاظم
Supervisor name: عبد السادة عبد العباس راهي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: English
University location: Wasit
First pages:
Abstract: في الدراسة الحالية، نصف طريقة سلسلة تفاعل البلمرة المتسلسل للكشف عن DNA محدد من طفيلي المشعرات المهبلية في الفحص السريع لتقنية الوقت الحقيقي لسلسلة تفاعل البلمرة. وتقييم مدى انتشار داء المشعرات وفقا مع دراسة العوامل المختلفة التي تؤثر على داء المشعرات الم | This study was involved the describe of an existing PCR method for specific detection of Tichomonas vaginalis DNA into a rapid real - time PCR assay, and to evaluated the recent prevalence of trichomoniasis accordance with studying different factors that affect on vaginal trichomoniasis in females complaining of vaginal discharge.Vaginal swab samples were collected from 401 patients attending two public hospitals and three private clinic in the Al - Kut city. Demographic data, clinical and socioeconomic status were collected from the patients using a structured questionnaire. Total genomic DNA was isolated from 60 samples of vaginal swab randomly chosen to specific detection of T. vaginalis DNA into a rapid real - time PCR.All samples were examined by the direct microscopic examination (wet mount and Giemsa - stained smear). Their ages were ranging from 14 - 63 years. The vaginal swabs examined by wet mount and Real Time - PCR to detect the presence of T. vaginalis. The pH strips were used to detect the vaginal acidity.Of the 60 women, 13 were positive by Real Time - PCR giving the prevalence rate of (21 67%) with sensitivity, specificity and accurate rate 100% and the direct microscopic examination (wet mount and Giemsa - stained smear) show 5/60 (8.33%) positive case while 55/60 (91.67%) gave a negative result with a sensitivity and specificity, and accuracy (38.5%) (100%) (86.7%).Women aged (24 - 33)years had significantly higher prevalence of trichomoniasis (46.1%) than other age groups.Married women had the highest percentage of trichomoniasis (76.9%), comparing with widowed women (7.7%) and divorced women (15.4). With statistical significant difference between these groups. Uneducated women had significantly higher rate of T.vaginalis infection (76.9%) than educated women (23.1%). Statistical significant difference was detected between these groups.Pregnant women showed a higher infection rate 7(53.8%) than non - pregnant 6 (46.2%).Women with vaginal pH (6 - 7), (5 - 6) had significantly higher rate of infection (53.8% and 38.5%) respectively.Women who used (loop and the oral contraceptive pills) contraception had the lower rate of infection(23.1%) than those not using any contraception (67.9%). Statistical significant difference was detected between these two groups.The highest percentage 2(66.67%) was found among women with T. vaginalis who used contraception for >2 years.The higher percentage of infected with T. vaginalis parasite was found in bad odor or fish odor vaginal discharge which was noted (76.9%) of infected women with T. vaginalis. Statistical significant difference was detected between these groups. Women with Frothy discharge color showed higher significantly percentage of infection 7(53.8%) than other with Yellow to green and bloody discharge and Clear discharge 3(23.1%), 2(15.4%) and 1(7.7) respectively.Multipara women revealed the highest rate of trichomoniasis (46.15%). No statistical significant difference was detected between infection and parity.T.vaginalis was more commonly seen in women with frequency of sexual intercourse 2 - 3 time per week 5 (50%) cases. The lowest incidence was detected among those who had sexual intercourse once per month 1 (10%), with statistical significance among these variables. T.vaginalis was found to be predominant among housewives (69.23%) than employees (30.77%).Fertilizing women were showed higher 9(69.2%) T. vaginalis infection than non - fertile women 4(30.8%).The high incidence of T.vaginalis infection was found among rural females 8 (61.5%), followed by urbanized females 5 (38.5%).

تقييم فعالية المستخلص المائي والكحولي لنبات الصبار Aloevera في الاصابة التجريبية لطفيلي Eimeria tenella لدى فروج اللحم == Evaluationof Alcoholic Andwateryextracts Efficacyofaloe Veraon Experimental Infectedbroilerwitheimeriatenella

Author name: زهراء حاتم حميدي عبود الكعبي
Supervisor name: هيثم محمد حمادي العوادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: اجريت الدراسة الحالية على (216) فرخ دجاج نوع Rose 308مصاب بطفيلي Emiria tenella خلال المدة من شهر نيسان 2013 الى شهر تشرين الاول 2013, وقد تم في الدراسة الحالية تحديد بعـــــــــض المؤشــــــــــــــرات الدمــــوية (الخلايا المتغايرة /الخلايا اللمفاوية HL | This study was conducted to (216) broilers chickens (Rose 308 Trade) infected parasite with Eimeria tenella performed form April 2013 till October 2013, has been in the current study to identify some indicators of blood(Heterophils/ Lymphocytes, White Blood Cell, Red Blood Cell, Packed Cell Volume, Hemoglobin) and biochemistry (Glucose, cholesterol and total protein) and productive performance (Weight increase, Food conservation, Cansumbtion and Body weight befor slugtery) , We note at the parasite has infected chicks were used three extracts of Aloe vera (alcoholic and hot water and cold water), dosage chicks were to demonstrate the effect of different concentrations of these extracts on the productivity and physiological traits mentioned above. Then the experiment was designed, which include the following : 1 - The first group (control group) included 36 chicks and was given food with no anti - coccidial. 2 - The second group include 36 chicks in duplicate was infected but did not treated.3 - The third group inclouded 36 chicks in duplicate was given extract of Aloe vera Alcoholic ethyl in adose of (0.5 - 1.5 - 2)gm per mm body weight.4 - The fourth group inclouded 36 chicks in duplicate was given extract of Aloe vera Cold water in adose of (0.5 - 1.5 - 2)gm per mm body weight.5 - The fifth group inclouded 36 chicks in duplicate was given extract of Aloe vera Hot water in adose of (0.5 - 1.5 - 2)gm per mm body weight.6 - The sixth group include 36 chicks in duplicate was given Vetacox(trade Mark)that had both Sulphadimidin 80 gm and Diverdin8 gm.Results showed the following : - 1 - The alcoholic extract of Aloe vera at concentration (0.5, 1.5, 2) g/ml after an infection in the fifth week revealed alittle effect on the H/L when compared with eighth week of infection. also this extract lead to increase, the rate of (PCV), (Hb) , and (RBC) while lead to decrease the rate of (WBC) after eighth week of infection at concentration (2)g/ml. Also this extract lead to increase the rate of cholesterol, total protein and the rate of weight increased in the eighth week, while the amount of feed intake and feed conversion showed alittle effect, as well as the body weight befors slaughter has astrong effect at concentration(2)g/ml in the eighth week of infection. 2 - The cold and hot aquatic extract of Aloe vera at concentration(2)g/ml lead to decrease the ratio of H/L and increase the rate of (PCV), (Hb) and (RBC) after the infection in the eighth week, also lead to decreas the rate of (WBC) and increase the rate of cholesterol, total protein, the rate of weight increased, the amount of feed intake and the body weight before slaughter at concentration (2)g/ml in the eighth week of infection. Also recorded abetter efficiency of feed conrersion at concentration (2)g/ml in the eighth week of infection.

انتشار داء المقوسات العيني مع تقييم لبعض جوانب الحالة المناعية في عينة من المرضى العراقيين == Prevalence of Ocular Toxoplasmosis And Evaluation of Some Immune Status Aspects In A Sample of Iraqi Patients

Author name: عبير فاروق ساكن الحكيم
Supervisor name: اسراء قاسم العبيدي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: اجريت الدراسة الحالية على 104 مريضا من المراجعين لوحدة الشبكية في مستشفى ابن الهيثم التعليمي للعيون في بغداد للمدة من بداية شهر كانون الاول 2013 لغاية شهر حزيران 2014. قسمت مجموعة المرضى استنادا للتشخيص السريري وفحص اختبار التلازن Latex test الى 50 مصابا | The current study was conducted on 104 patients whose underwent to the retina unit in Ibn Al - Haitham Hospital of Eyes in Baghdad from December 2013 to May 2014. The patients groups were divided according to the clinical diagnosis to 50 patients infected with ocular toxoplasmosis (OT) and 54 patents with uveitis, in addition to these groups acontrol group was also included 96 healthy apparent persons without any infection in their eyes.Some epidemiological characters were studied like age, that showed a little effect on two groups OT (Mean of age 38.07 ± 15.14) and uveitis (Mean of age 35.1 ± 17.6). The two groups were found in range 20 - 40 year while the control group include individuals with different age due to the randomly collection. The occupation factor play arole in the prevalence of infection with highly significant differences (P<0.05) in (house wife, workers) with a percentages 35(70%) 35(64.8%) in OT and uveitis groups respectively but the control group expend to include house wife, workers and officers with a total rate 92.7%.The education level also showed significant differences (P<0.01) among the studied groups, the most of ocular toxoplasmosis and uveitis samples were found in highly frequency at the primary, intermediate and secondary educational level with total rate 74% and 77.81% respectively while the control group was found at the secondary and under graduate educational level in 77.1%. The residency character was also examined and no revealed significant difference (P<0.05).The infection path was also set by the presence of animals especially cats which ware existed leading to statistical differences, p? 0.01 for OT group with rate 18%. The visual activity character showed no significant difference (P?0.05) between the patients of OT and uveitis who affected their visual acuity in right eye while the doubling response was recorded for uveitis patients with rate 55.6% in comparable with OT patients with rate 38% for the left eye. The intra ocular pressure was normal in both groups and for both eyes without significant difference.Latex and ELISA IgG. IgM serological tests were used in this study. The results were showed that latex test is easier and it is appear that OT patients were diagnosis with 100%, while the uveitis patients didn't show any positive result. Control group showed that 21 individuals were infected with rate of 21.9%.ELISA - IgG toxo. antibodies confirmed that the infection rate of OT patients was 39 (78%) versus 11(22%) with negative result, while the study also confirm that uveitis patient that have OT disease was 12 patient from 54 case, 42 case didn't diagnosis for OT. The control group was re - evaluated showed 28 case with toxoplasmosis infection from 96 case which from the subclinical group. The antibodies levels were measure by international unit/ ml for all groups, the IgG level was 3.186 ± 1.703 Iu/ml in positive control group followed by OT patients with 2.927 ± 2.417 and uveitis group 0.637 ± 0.803, finally the negative control group 0.276 ± 0.163. IgM levels were low and its presence didn't have any positive case. This study also showed that there was a relation between the IgG level and the pathological effect on eyes which resulted from infection for both groups OT and uveitis, it was high rate 73.5% with 50 patient, for posterior uveitis causing significant differences (P<0.05) followed by anterior uveitis with rate 13.2%.As for the results of cytokines there was a high level of IFN - ? in positive control group 793.7 ± 3461.6 pg/ml followed by OT group 550.6 ± 210.6 pg/ml, the uveitis group that in infected with ocular toxoplasmosis and negative control recorded 524.1 ± 173.6 and 506.6 ± 104.6 pg/ml levels respectively, this rise in the level of IFN - ? cause a significant differences at (P<0.05) for OT, uveitis (non infected) and positive control groups. TNF - ? recorded arise of its level in positive control group 34.89 ± 12.29 pg/ml followed by OT group 32.24 ± 9.91 pg/ml then positive uveitis group 23.61 ± 12.91 pg/ml after them negative control 14.62 ± 5.43 pg/ml finally negative uveitis group with 7.76 ± 4.04 pg/ml.High level of 1L - 12 was gotin positive control group in a concentration 827.00 ± 224.25 pg/ml followed by OT group with 811.28 ± 179.40 pg/ml. An increase in 1L - 17A was recorded in positive control group with mean 327.65 ± 77.89 pg/ml while converged concentration in OT group 263.73 ± 47.48 and positive uveitis group 252.57 ± 61.55 pg/ml. The lowest level was in negative control group 421.6 ± 168.14 pg/ml. MCP - 1 showed a rise in its level in positive control group 274.09 ± 92.05 pg/ml and reduce in OT patients 217.68 ± 98.70 pg/ml, while the two groups negative and positive uveitis recorded levels 155.71 ± 99.51 and 132.60 ± 99.02 pg/ml respectively. The lowest level was register in negative control 97.56 ± 79.17 pg/ml.

مقارنة لتشخيص الخمج بالمقوسات الكوندية بين النساء المجهضات والولودات طبيعيا مع دراسة للتغيرات النسجية المتسببة عن الخمج == Comparative Diagnosis of Toxoplasma Gondii Infection Among Aborted & Normally Delivered Women With Study of Histological Changes As A Result of Infection

Author name: فراس محمد بشير عبد الكريم الخشاب
Supervisor name: ابراهيم شعبان داوود | صباح سعيد محمود
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت هذه الدراسة التي امتدت من بداية شهر ايار (2007) لغاية نهاية شهر اب (2008) للتحري عن نسبة الخمج بالطفيلي المسبب لداء المقوسات بين النساء المجهضات والولودات طبيعا في بعض مناطق مدينة الموصل والمراجعات لمستشفى الخنساء التعليمي للولادة والاطفال.شملت عي | This study was conducted during the period from May 2007 to August 2008 to investigate the rate of infection with toxoplasmosis among aborted and normally delivered women in Mosul City territories who attending AL - Khansa'a educating hospital.Out of 150 women under study, 50 were aborted while 100 were normally delivered. The employed tests were included latex agglutination test LATEX and Enzyme Linked Immunosorbent Assay (ELISA) with its two types (ELISA - IgM), (ELISA - IgG) which used as a high specific and sensitive test to differentiate between types of infection acute, chronic and congenital. Toxoplasmosis was diagnosed in 28(56%) out of 50 abortive placenta and in 57(57%) out of 100 non abortive placenta (normally delivered women), these samples of placenta were microscopically examined after homogenizing and staining by Gimsa and Leishman's stain The parasite development stages were also noticed including developing from the tachyzoites into the oocysts stage and the histopathological effects resulting from infection in the placenta in both aborted and normally delivered women which were represented by necrosis, vaculation and cells degeneration , inflammatory cells infiltration and the focal inflammatory reaction in the villi in addition to hemorrhage and accumulation of eosinophilic substances, the results also showed that 41 (82%) of aborted placenta and 18 (18%) of non abortive placenta (normal delivered women) were infected with Toxoplasmosis by using the laboratory Balb/c mice inoculation method while, some cases of inflammation were diagnosed in the brain, of the experimental infected mice, which were represented by the inflammatory cells infiltration and the prevailing of gliosis, tissue necrosis vaculation and cells degeneration.This study revealed that laboratory mice inoculation in the peritoneal cavity is a useful and efficient method in the detection of the parasite in infected placenta as compared with the microscopic examination of direct smears and examination of tissue sections from placenta.The present study showed an overall percentage of infection among aborted women was (92%) while (58%) showed an acute infection and (34%) chronic infection, in another hand the infection rate among normally delivered women was (70%), and it has been found the infection rate among acute and chronic cases were (41%), (20%) respectively, Its to be noted this is the first time in Mosul City for such a study conducted in this area concerning the congenital Toxoplasmosis in newborns with an infection rate (9%).In this study, sera were tested by Latex (LAT), and Enzyme Linked Immunosorbent Assay (ELISA). It was shown that IgG - ELISA is the most sensitive to diagnose the infection as compared to other tests.These study also concerned with the isolation and diagnosis of Toxoplasma gondii parasite from blood by inoculating the blood samples in laboratory Balb/C mice the rate of infection was (16%) in laboratory mice inoculated with blood collected from aborted women while the infection rate in laboratory mice inoculated by blood samples (mother and her neonatal cord blood) collected from normally delivered women appear in (12%) & (16%) respectively. The rate of occurrence of the specific antibodies against Toxoplasma gondii was identified, The highest rate was found for the type (IgG+ - IgM+), in both aborted and normally delivered women with percentage reach (40%) & (26%). The titer (100 IU/ml) of the specific IgM & IgG antibodies was found in high rate reached (75%), (43.5%) in mother blood, also found in a high percentage in the neonatal cord blood estimated as (50%), (64.4%) for the specific IgM & IgG antibodies respectively, in another hand the titer (100 IU/ml) was also found to be the more predominate titer in blood samples of aborted women with percentage reached to (50%) & (60.5%) for the specific IgM & IgG antibodies respectively.

دراسة مقارنة للكشف عن الجيارديا لامبليا والطفيليات المصاحبة لها بين الاطفال في مدينة كركوك باستخدام بعض التقانات المختبرية == A Comparative Study For Detecting Giardia Lamblia And Associated Intestinal Parasites Among Children In Kirkuk City By Using Some Laboratory Methods

Author name: مها اسماعيل مصطفى الجبوري
Supervisor name: يحيى جرجيس سلمان
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: English
University location: Kirkuk
First pages:
Abstract: The current study had been carried out from January 2013 to July 2013 in medical laboratory researches - Kirkuk Faculty of Medicine. A total of 310 stool samples have been collected from children suffering from liquid diarrhea, their ages are from below one year to 12 years; six different laboratory diagnostic methods have been applied for detecting Giardia lamblia and other intestinal parasites. For microscopy diagnosis; direct wet double prepartions, zinc sulphate flotation are used. While immunological methods involve; Enzyme Linked Immuno - Sorbent Assay, corpo - antigen (ELISA), Direct Fluorescent Assay (DFA) and Lateral immune - chromatography assay (Triage panel). Giardia genome amplification is done using conventional Polymerase Chain Reaction (PCR) single step procedure by using of mixed primers of Giardia assemblages A1, A2 and B. The total rate of intestinal parasitic infection is 51.93 % distributed in 161 stool samples. This rate involve high frequency of protozoan infection in 132 (42.58 %) compare to 29 (9.35 %) for helminthes, P<0.05. More common intestinal protozoan parasites were Giardia lamblia 63(20.32 %) followed by Blastocyst hominis, Cryptosporidium parvum, Entamoeba coli, Entamoeba histolytica, Iodomoeba butschili, Endolimax nana and Balantidium coli with the rates : 6.77%, 6.45%, 4.18 %, 2.58 %, 1.29 %, 0.64 % and 0.32 % receptively. Concerning intestinal helminthic infection, high number and rate 23(7.41 %) was with Hymenolepis nana is compare with 1(0.32 %) record for Ancylostoma duodenale.According to gender high rate of giardiasis is recorded among males than in females, conversely to high rate of other intestinal parasites among female than in males P<0.05. Statistically relationship between Giardia distribution and ages are not significant P>0.05. Giardia co - existence are highly detected with Balstocystis hominis, and Cryptosporidium parvum total Giardia mixed parasitic infection rate 8.06 % is lower than12.23 %of pure Giardia lamblia infection, P>0.05. Regarding Giardia lamblia detecting according to laboratory methods; high rate of giardiasis 22.29% is reported by using PCR technique, followed by 20, 23 % by using direct wet preparation technique, P<0.05.The efficacy of laboratory methods for detecting Giardia stages are reduced; the following rates 19.35%, 19.03%, 17.74% and 14.51% obtained by using ELISA, DFA, flotationand lateral immuno - chromatography assay (Triage) respectively, P<0.05. Also statistical analysis reveals significance of PCR sensitivity, specificity, and accuracy in detecting giardiasis than other laboratory methods. Negative predictive values NPV in relation to type of laboratory methods are high, but statistically they are not significant, controversy to positive predictive value PPV that are significant. The efficacy of Triage panel is high for detecting giardiasis 14.45 % as compare with 4.5 % and 3.5 % for detecting Cryptosporidium parvum oocysts and Entamoeba histolytica respectively P<0.05 %. Considering the application of double direct wet preparations, the results of using this method are beneficial for detecting protozoan and helminthic parasites. While the using of zinc sulphate flotation technique reveal fluctuated results in spite of significant statistical analysis P<0.05. The employee of five laboratory methods for detecting the oocysts of Cryptosporidum parvum; the following rates 6.45%, 5.48%, 4.83%, 4.51% and 3.22% are recorded with the usage of DFA, direct microscopy, modified Ziehl - Neelsen method, Traige panel and flotation method respectively, P>0.05. Giardia lamblia DNA extraction from 80 stool samples that amplified using Giardia gene loci K725, reveal 66 samples positivity, pure Giardia lamblia genomic mass mean is 437.56 bps, with 1.705 % of genome purity and the extension of genomes range from 280 to750bps.While 23 of mixed Giardia plus other protozoan parasites, the mean of gemonic mass is 439.89 bps with genome purity mean 1.56 %. Amplification of Giardia genome mixed with helminthes reveal 443.33 bps of genomic mass mean and 1.49 % genome purity mean. In general the all genomic mass of Giardia lamblia (Pure and mixed infection) is 440.26 bps and purity mean 1.54 % P>0.05.PCR amplification in stool sample exert that Giardia lamblia genomes are mixed of human and animal type.

تاثير الاصابة بداء المقوسات الكوندية على المستويات الهورمونية والمدورات الخلوية خلال فترة بلوغ الانسان في محافظة بغداد == The Effect of Toxoplasmosis On Hormonal And Cytokines Levels During Human Maturity In Baghdad Province

Author name: ياسمين رياض عبد الكريم الخناق
Supervisor name: صباح ناصر العلوجي | خولة حوري زغير
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: داء المقوسات الكونديه مرض عالمي الانتشار حيث يصاب معظم الاشخاص ذوي المناعة الجيدة بطفيلي المقوسات الكونديه وغالبا دون ظهور اعراض. ان الهدف الرئيسي لهذه الدراسه هو فهم الفروقات الجنسيه, الهرمونيه والمناعيه في سن البلوغ في الاشخاص الذين لديهم اجسام مضاده نو | Toxoplasmosis is a worldwide disease where most healthy, immunocompetent individuals infected by Toxoplasma are almost asymptomatic.The primary goal of this study is to perceive the hormonal and immunological sex - differences in puberty age who have positive anti - Toxoplasma IgG specific antibodies. The secondary goal is to inspect the endocrine - immune interaction in these persons by detecting the effect of testosterone and oestradiol hormones level on cellular immune response namely, IL - 4, IL - 12. From the first of November 2012 till the end of April 2013, 303 blood samples were collected from apparently healthy male and female students of Al - Erfan, Ignadeen and Algawahery schools and Baghdad University, Both sexes where divided into two age groups : group (A) which included subjects with age range (12 - 15) years old and group (B) which included subjects with age range (16 - 19) years old. All serum samples were tested for toxoplasmosis by using Latex agglutination test and ELISA anti - Toxoplasma IgG antibodies test. As well as, all serum samples were tested by using ELISA technique for detection of serum mean concentration of testosterone, oestradiol hormones, IL - 12 and IL - 4. The results revealed that 107/ 303 (35.31%) of the studied subjects showed seropositive toxoplasmosis, 60 males and 47 females of 107 positive samples showed high significant (p<0.01) differences in comparison to uninfected subjects. Males group B have recorded the highest percentage 34(41.46%) of the infection. Positive association was found between toxoplasmosis and testosterone level in asymptomatic toxoplasmosis cases compared to uninfected group. high mean concentration of testosterone in toxoplasmosis infected males recorded (15.03± 1.04 ng/ml) and (12.4± 0.91 ng/ml) in groups A and B respectively, in comparison to control group which recorded (8.03± 0.78) and (9.86± 0.83) in groups A and B respectively. Also toxoplasmosis infected females revealed high levels of testosterone hormone which represented (4.83± 0.06 ng/ml) and (2.55± 0.03 ng/ml) in groups A and B respectively, with a significant (p?0.05) differences between them, while the control group recorded (0.10± 0.02 ng/ml) and (0.90±0.03 ng/ml) in group A and B respectively.The present study showed a significant (P?0.05) decrease in the mean concentration of oestradiol E2 hormone in toxoplasmosis infected males and females in comparison with uninfected ones. E2 mean concentration was (41± 2.48 ng/ml) and (56± 2.91 ng/ml) for male groups A and B, respectively, in comparison to control group which recorded (67± 2.08 ng/ml) and (74± 2.42 ng/ml) in group A and B respectively, while it was (188 ± 12.48 ng/ml) and (196 ± 16.52 ng/ml) for female group A and B respectively, in comparison to control group which recorded (221±12.09 ng/ml) and (233± 15.63 ng/ml) for group A and B, respectively.The mean concentration of E2 hormone in toxoplasmosis infected females according to their menstrual cycle showed low levels in ovulation, late follicular and luteal phases, which represent (37.5 ± 2.59 ng/ml), (131 ± 16.7 ng/ml) and (76± 3.92 ng/ml) respectively, while the mean concentration of this hormone in uninfected females was (52.4± 2.88 ng/ml), (271.6± 21.04 ng/ml) and (196.2± 12.37 ng/ml) in the three phases of menstrual cycle. This study showed high significant (p?0.05) level of IL - 12 in both males and females with latent toxoplasmosis in comparison with free - toxoplasmosis groups. The mean concentration of IL - 12 in infected males was (4.75 ± 0.88 pg/ml) and (4.12 ± 0.69 pg/ml) in male groups A and B respectively, in comparison to control group which was (2.86± 0.53 pg/ml) and (2.46± 0.62 pg/ml) in groups A and B respectively, while it was (5.60 ± 0.12 pg/ml) and (6.04 ± 0.26 pg/ml) in infected female groups A and B respectively, in comparison to control group which recorded (3.32± 0.89 pg/ml) and (4.27± 0.15 pg/ml) in group A and B respectively. IL - 4 recorded quite elevated level in toxoplasmosis infected males (groups A and B) which was (15.09 ± 0.92 pg/ml) and (17.67 ± 0.78 pg/ml) respectively, in comparison to control group which recorded (13.89± 0.84 pg/ml) and (14.92± 0.69 pg/ml) in groups A and B respectively, Meanwhile the mean concentration of IL - 4 in toxoplasmosis infected females showed mild elevation in both groups A and B which was (15.14 ± 0.84 pg/ml) and (16.06 ± 1.13 pg/ml) respectively, in comparison with toxoplasmosis free subjects which recorded (16.53± 1.22 pg/ml) and (15.15 ± 0.97 pg/ml) with no significant differences between them. Interactions between the endocrine and immune systems may mediate sex differences in response to toxoplasmosis infection.

تقييم فعالية قاعدتين من قواعد شف الجديدة في بعض انسجة ذكور الفئران المختبرية البيض المصابة وغير المصابة بطفيلي المتحولة الحالة للنسج Entamoeba histolytica == Evaluate The Effectiveness of Two Bases of The New Schiff Bases In Some Tissues of Male Laboratory Albino Mice Infected And Non - Infected With The Parasite Entamoeba Histolytica

Author name: عبد الجليل عزيز كريم
Supervisor name: فاضل عباس منشد العبادي | حيدر عباس مهدي الحسيني
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: تضمنت الدراسة الحالية تحضير وتشخيص قاعدتين من قواعد شف، حضر الليكاند الاول L1 من تفاعــل 2 - هيـدروكسي - 1 - نفـثالديهايد مــع الـسلفاميثوكسازول، وحضر الـثانـي L2 مـن تـفاعــل 4 - استميدوبنزالديهايد مع 4, 3 - داي امينو تلوين. شخص الليكاندان باستخدام ت | The present work includes a preparation and characterization of two schiff bases compound, the first from (L1) reaction of 2 - hydroxy - 1 - naphthaldehyde with sulphamethoxazole, and the second from (L2) reaction of 4 - Acetmidobenzaldehyde with 3, 4 - Diamino toluene. These compounds were characterized by the Infrared (IR) Spectroscopy, Nuclear Magnetic Resonance Spectroscopy (1H - NMR), Mass Spectra, and the results were virtually identical to what is expected. It has been determined LD50 for the two ligands - after dissolved dimethyl sulfoxide DMSO - and account for them safe therapeutic dose, reaching 0.14 g / kg and 0.24 g / kg, respectively. In order to see the effectiveness of the two ligands on the parasite compared with the infected group and treatment with a drug metronidazole, these two ligands have been administered orally the therapeutic dose each separately on two groups of mice which infected by the parasite Entamoeba histolytica. Three other groups of mice that non - infected have been also administered separately with the parasite first and second ligands and dimethyl sulfoxide, to see some of the side effects of these three compounds in comparison with the two groups of positive and negative control. At the level of probability (p ? 0.05), the results of statistical analysis show a significant variation, remarkable inhibitory effect of the number of cysts that reached the less impact to the second ligand rate about 2.9. in the seventh day of the injury and treatment. In the fifth day of the injury and treatment, the highest inhibiting effect of metronidazole was at a rate of 0, while in the seventh day of them, the first ligand was a compromise in effect, amounting to at a rate of 1.1. The Histological study of tissue of the cecum, liver and spleen showed a normal state for a negative control, and an occurrence of severe tissue changes in a positive control. In the cecum tissue, the mucosal was thin, and there were crashes in the crypts of Lieberkühn, with infiltration in inflammatory cells, as well as the expansion of the cells lining the crypts, and the disappearance of or crash submucosal layer, as well as severe hemorrhage in muscularis, and thickening in serosa. There was severe congestion of the blood vessel which was observed in liver tissue, as well as thrombus which appeared attached to the wall of the blood vessel, and infiltration of inflammatory cells out of the blood vessel toward the hepatic tissue, especially macrophages and eosinophils. It was also noticed the disappearance of hepatic architecture with degeneration of the liver cells and a clear sinusoids expansion. In addition to vacuolation of liver cells, that the tissue sections showed, with the occurrence of bleeding along the hepatic tissue. The changes in the spleen tissue represented in the occurrence of severe bleeding on the length of the lymphatic tissue of the spleen with infiltration of inflammatory cells, especially macrophages, and the happening of megakaryocytes. There is a remarkable improvement could be noticed in each tissues of cecum, liver and spleen of the infected groups and treatment with metronidazole and the first and second ligands. The improvement ratios were close to some extent : in the treatment group with metronidazole there was observation in tissue cecum to the occurrence of the four layers of its component, and the happening of a little bleeding which, with infiltration of inflammatory cells, and this is roughly similar to what shown by histological sections of two treatments by the first and second ligands. The liver tissue of the group treated with metronidazole showed the occurrence of radially arrangement obviously hepatic cells around the central vein, and liver cells with nuclei appeared clear and centralized with esinophilic cytoplasm, and hepatic cells proliferative as some binucleated hepatocytes, with a simple expansion to sinusoids and the proliferation of kupffer cells, the treatment groups with two ligands marked by the presence of a small clot within the central vein of the liver as well as reported in the treatment group with metronidazole. Tissues sections of the spleen tissue of infected mice treated group by metronidazole showed a clear proliferation of white pulp, which appears containing arterioles clear, white pulp is also surrounded by proliferation red pulp. And showed tissue sections of the group of infected animals and treatment by the first and second ligands as well as the proliferation of lymphocytes, with the presence of megakaryocytes.Showed the three groups non - infected by the first and second ligands and dimethyl sulfoxide, the lack of side effects on each of the cecum tissue, liver and spleen, except for a very simple infiltration of inflammatory cells from macrophages in the cecum of the group treatment by the second ligand

دراسة التغايرات النسجية والمناعية لمرضى اللشمانيا الجلدية في محافظة ديالى == Study The Histological And Immunological Variation of Cutaneous Leishmaniasis Patients In Diyala Province

Author name: منى حميد احمد العزاوي
Supervisor name: اسراء قاسم العبيدي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: اجريت الدراسة الحالية على المرضى المصابين بداء اللشمانيا الجلدية والمراجعين لبعض المستشفيات والمراكز الصحية في محافظة ديالى للمدة من تشرين الاول 2013 ولغاية نيسان 2014. اذ بلغ عدد المصابين باللشمانيا الجلدية 115 مصابا وبمختلف المراحل العمرية يضاف لهم 25شخ | The present study was conducted on patients that infected with cutaneous leishmaniasis reviewing to some hospitals and health centers in Diyala province for the period from October 2013 to April 2014.The number of patients with cutaneous leishmaniasis was 115 in different ages in addition to them 25 person as a control group (uninfected). The proportion of infected women with cutaneous leishmaniasis ulcers was higher 56.522% than males 43.47%, the age group ten years and less was more group that being infected with rate 47.83% while the age group that rang from 71 to 80 was the less categories being infected in percentage 0.87% with a significant differences in the level of improbability less than 0.05. The presence of multiple ulcers was high 71.304% than single ulcers 28.696%. The presence of ulcers that length 1 cm and less (64%) more than other with more than 1 cm (36%) with statistically significant. The cutaneous ulcers were distributed on different areas of the body, the injuries on face was more 36.242% with anumber of 54 cases followed by upper limbs in rate 33.557% with anumber of 36 cases, the sore shoulder came latter with apercentage 1.342% in a significant differences at the level of improbability less than 0.05. The percentage incidence of cutaneous leishmaniasis was rise in kids and students in rats 33.043%, 39.130% respectively in a comparison with earners, housewives and of officers their percentages were 24.349% and 3.478% consecutively in statistically differences at 0.05 probability. The diagnosis of cutaneous leishmaniasis infections was done depending on clinical diagnosis based on the characteristics of the morphological and pathological ulcers, culture methods, impression smears and histopathological diagnosis. Clinical diagnosis for the cutaneous ulcer was recorded 100% percentage in comparison with culture method 10.435% versus 74.782%. percentage to positive smears, while the result of positive histopathological diagnosis was 88.571% on vision of promastigote of Leishmania parasite on semisolid medium while a adoption of existence amastigote from as appositive result of smears and histopathological method of the tissue biopsies taken from ulcer. The histopathological method was high efficiency supported clinical diagnosis when compared to other methods this rise in the diagnosis ratio has been demonstrated in significant differences in statistical probability less than 0.05.A study of amain impacts of histopathological changes in cutaneous ulcers on the infected skin layers at the level of light microscopy was also conducted. These pathological effects were in epidermis of skin including hyperkeratosis, pseudoepitheliomatous epidermal changes, ulcers and emergence of a dark colour, thickening in this layer accompanied suppurations and desquamation of chronic cases. As for the dermis layer, the inflammatory cells were infiltrate include plasma cells, macrophages, lymphocytes and eoisophils, disturbance in the order of layers especially in acute ulcers was taken, while the pathological effects were more in chronic ulcers as observed granulomatous inflammation and necrosis. The amastigote from were presence inside and outside the phagocytic cells.Aswell as some immunological changes that associated with the infection were studied in patients therapists by Pentostame (Sodium Stubogluconate) medication and uninfected control group by measuring the levelsof some cytokines IFN - ?, TNF - ?, IL - 12, IL - 17A and the chemokine MCP - 1. The level of IFN - ? was elevated in serum of cutaneous leishmaniasis patients and reached to 54.118±1.740 pg/ml, then the level was decreased during therapy and recorded 34.421±0.984 pg/ml after 1 - 3 dose a then dropped more after 4doses and more 22.489±0.795 pg/ml compared to uninfected control group 5.092±0.805 pg/ml. As well as the case of TNF - ? which rose their concentration in serum of CL patients and reached to 98.145±2.925 pg/ml, then decrease after treatment 71.507±2.901 pg /ml, 48.381±2.379 pg/ml after 1 - 3dose and more of treatment respectively, while the mean of TNF - ? in control group was 13.173±1.388 pg/ml.An increase in the level of IL - 12 was observed in the group of CL patients 845.598±16.273 pg/ml then the level was dropped during treatment and recorded 619.164±20.209 pg/ml, 495.578±27.038 pg/ml respectively but in control the concentration was 160.222±11.761 pg/ml. IL - 17A level was elevated in CL patients 227.268±5.027 pg/ml and decreased during therapy reach to 170.512±5.481, 126.443 ± 5.565 pg/ml after 1 - 3 doses and four doses and more respectively. The concentration of MCP - 1 chemokine was increased in CL patients 111.0397± 6.985 pg/ml then dropped during treatment 71.084± 2.636 pg/ml after 1 - 3 doses then dropped more 49.543± 2.167 pg /ml after four doses and more while they were in control 12.268 ± 1.609 pg/ml. The results of statistical analysis showed significant differences between these cytokines and their groups at the level of probability less than 0.05.

دراسة تشخيصية وامراضية نسجية للقناة الهضمية في الدجاج المنزلي Gallus gallus domasticus المصابة بالطفيليات المعوية في مدينة البصرة == Diagnostic And Histopathological Study of Intestinal Tract of Domestic Fowl Gallus Gallus Domesticus Infected With Intestinal Parasites At Basrah City

Author name: اسراء صبيح بداي الغنامي
Supervisor name: غازي يعقوب عزال الامارة
General topic: Veterinary Medicine
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: امتدت الدراسة الحالية من شهر تشرين الاول 2011 ولغاية شهر اذار 2012 , حيث جمعت خلالها 108 نموذجا بواقع 36 عينة من الذكور و72 عينة من الاناث من الدجاج المحلي1758) Gallus gallus domesticus (Linnaeus, من محافظة البصرة بهدف عزل الديدان المتطفلة على القناة الهض | The recent study extended from October 2011 to January 2012, collected 108 samples by 36 samples of males and 72 samples of female of local chickens Gallus gallus domesticus (Linnaeus, 1758)from Basrah to isolate parasitic worms on the gastrointestinal tract and diagnosed as well as study percentage and severity of the infection, as well as monitoring biological activities of isolated parasites and find out macroscopic and pathological changes caused by these worms and find out the impact of tapeworms on the values of the blood parameters of chicken. The results showed infected of chicken with five types of tapeworms (Hymenolepis carioca, Raillietina tetragon, Raillietina cesticillus, Choanotania infundibulum, Cotugina sp) with percent of infection and severity reached 4.62%, 11.4; 19.44%, 12.09; 5.55%, 12.66; 9.25%, 12.6; 6.48 %, 11.14 respectively and got one type of nematodes Subulura brumpti with percentage of infection and severity reached 10.18%, 91 and three types of trematode Echinostoma revulatum, Hypoderm coniodium, Psilolecithum longorchum with percent of infection and severity reached 1.85%, 15.5; 0.92%, 10; 0.92% , 7 respectively.The results of the recent study showed that infection with two types is the most common reaching incidence of 25.92%, and study results indicated that there were significant differences between males and females in susceptibility to intestinal worms.Observed by studying the sites of worms in the gut that the proportion of tapeworms in the small intestine are more than in the large intestine while recorded nematodes only in the large intestine As for worms Trematode has found in the small intestine. The results also included monitoring biological activities of isolated parasites from the gastrointestinal tract from the beginning of the division of the egg and develop into a larva has been observed that the nematodes have the ability to survive outside the host, who were present more than tapeworms.the study contained a physiologic side on the impact of tapeworms on some blood parameters where showed infection of domestic fowl with anemia by observing the decrease in the number of red blood cells R.BC , hemoglobin Hb and P. CV while there was an increase in the number of white blood cells WBC. The study recorded the number of grossly and histological lesions in the intestine and the cecum of domestic chicken Because they infected with tapeworms, nematodes and flukes. grossly lesions included blockage and swelling in the intestines infected with tapeworm and cecum infected with nematode and appearance of rough nodules on the walls of the intestine infected with tapeworm with hemorrhage and the appearance of yellowish green purulent material in the inner wall of the intestines, and the histological changes has included atrophy of intestinal villi with sloughing and desquamation of epithelial layer with appearance of inflammation in the form of pseudomembrane and infiltration of inflammatory cells and coaglative necrosis, it have seen hemorrhage and superficial erosion in the mucous layer and sloughing of villi and epithelial layer with hyperplasia appearance purulent cells and destruction of intestinal glands and ulceration in the mucous layer in addition to the congestion

التحري عن داء المقوسات في النساء والنعاج بواسطة طرق مصلية مختلفة في محافظة ميسان - العراق == An Investigation of Toxoplasmosis In Women And Ewes By Different Serological Assays In Maysan Governorate, Iraq

Author name: مرتضى شهاب جبار
Supervisor name: منى محمد جوري
General topic: Veterinary Medicine
Specific topic: Microbiology - Parasites
Degree: Master
Language: English
University location: Basrah
First pages:
Abstract: تم جمع 291 عينة من الدم الوريدي من النساء في سبعة مناطق مختلفة في محافظة ميسان للفترة من تشرين الثاني 2012 وحتى اذار 2013 للكشف عن داء المقوسات باستخدام ثلاثة اختبارات هي اختبار التلازن الحبيبي وانزيم الادمصاص المناعي واختبار المنفايدس ثلاثة مجاميع من الن | In this study, venous blood sample were collected from 291 women in seven different regions of Maysan province during the period from October 2012 to March 2013 for detecting the toxoplasmosis by using three tests (Latex agglutination (LAT), enzyme linked immunosorbent (ELISA - IgG) and minividas. Three groups of women ( pregnant ,non - pregnant and aborted ) and 283 ewes which represent 18 herds from nine regions of Maysan province were selected for the study in order to diagnose the toxoplasmosis by using ELISA and LAT tests. The results showed that the ratio of Toxoplasma seropositivity in aborted women was significantly the highest (69.23%) followed by pregnant group (60.16%) by using LAT. Moreover, the ratio of seropositivity was highest in aborted women (47.43%) followed by pregnant (36.58%) and non - pregnant (26.66%) groups, respectively by using ELISA test. With Minividas assay, the results showed the highest seropositivity percentage in aborted women (26.92%) followed by the pregnant (17.88%) and non - pregnant (11.11%) groups, respectively. According to regions of the study, there was higher seropositivity against T. gondii in women in Al - Musharah subdistrict by LAT (68.09%) and ELISA (44.68%) test, respectively. However, the seropositivity by using minividas assay in three regions in AL - Majar district, Al - Musharah subdistrict and AL - Maymona district were 23.52% , 23.40% , 23.52% , respectively in comparison with other regions in Maysan governorate. The significantly high (P? 0.05) seropositivity detected by LAT (72.63%), ELISA (50.52%) and minividas (28.42%) were in women of age group > 30 years. The results showed that the ratios of Toxoplasma seropositivity in aborted ewes group was the highest (73.33%) followed by pregnant group (65.38%) after using the LAT, while the seropositivity in aborted ewes detected by the ELISA test was 40%. The results from Al - Musharah subdistrict that showed highest seropositivity against Toxoplasma by using LAT and ELISA tests were 78.26% and 39.13%, respectively. The highest Toxoplasma seropositivity in the two - years age - group ewes was 65.57% and one - year age - group was 65.30% after using LAT test, while was 32.65% in the age - group more than a year by ELISA. The statistical analysis showed significant difference (p ? 0.05) between the LAT and ELFA tests conducted for women samples, but there were no significant differences (p? 0.05) between LAT and ELISA carried out for women and ewes samples with regarded to Toxoplasma seropositivity.

الكشف الجزيئي والمصلي لداء المقوسات في الانسان والاغنام في محافظة واسط == Molecular And Serological Detection of Toxoplasma Gondii In Human And Sheep In Wasit Province

Author name: عباس حسن خلاطي السراي
Supervisor name: نعمان ناجي عايز
General topic: Veterinary Medicine
Specific topic: Microbiology - Parasites
Degree: Master
Language: English
University location: Qadisiyah
First pages:
Abstract: اجريت الدراسة الحاليه خلال الفترة من تشرين الاول 2013 الى ايار 2014 في محافظة واسط للكشف عن الاصابة T. gondii في الانسان والاغنام باستخدام الطرق الجزيئية والمصلية. تم جمع خمس مئة وثمانية عينات 500 عينه دم و8عينات من انسجه المشائم و305 عينه 300عينه دم و5ع | The present study was conducted during the period from October 2013 to May 2014 in Wasit province to detect the infection of T. gondii in women and sheep using Molecular and Serological methods.Five hundred and eight samples 500 blood samples and 8placenta and 305samples 300 blood samples and 5placenta were collected from both suspected women and sheep respectively. The sera samples were separated and examined by ELISA for human and Latex agglutination test for sheep to detect the infection with T. gondii serologically, then many blood and placental tissue samples (89 blood samples and 8 placental tissue samples for women, whereas 100 blood samples and 5 placental tissue samples for ewes) were subjected to polymerase chain reaction (PCR) technique to detect the infection molecularly.The Serological results showed that 17.8% of women were positive for the private screening ELISA detects toxoplasmosis (17 % with chronic infection and 0.8% with acute infection), it was noted that the highest rate of infection was in women who ranged in age between 20 - 29 years, reaching 19.9%, but with no significant difference (P>0.05) between the ages studied. On the other hand the rate of sheep infection by latex agglutination test with 2 - mercaptoethanol amounted to 33.33% (31.33% with chronic infection and 2% with acute infection) and most positive cases were among the ages which were equal to or more than 3 years (?3 years) with significant difference (P<0.05) between these ages. The present study indicated a lack of months effect on the distribution of parasite infection rates where these different months recorded relatively close rate ranged between 14.45% - 23.07% in women and 31.42% - 35.97% in sheep with no significant difference (P>0.05).Regarding to polymerase chain reaction test, a fragment of 399bp was amplified from B1 gene, the result showed that 6.74% and 4% of blood samples and 100% and 80% of placental tissue samples which taken from women and sheep respectively were positive to this test.

دراسة في الوبائية والتحري بتقنية لداء (PCR) سلسلة تفاعل البلمره الابواغ الخبيئة في شمال بغداد == Study In Epidemiology And PCR Detection Of Cryptosporidiosis In North Of Baghdad

Author name: حارث سعيد جعفر الورد
Supervisor name: احسان مهدي الصقر | سهيلة حياوي محمود
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

دراسة تشخيصية جزيئية ومناعية لانواع الاكريات Eimeria sp المخمجة للدجاج في محافظتي كربلاء المقدسة وبابل == Identification of Chickens Coccidian species by Molecular and Immunological Methods in Kerbala and Babylon Provinces

Author name: ضمياء مكي حمزة
Supervisor name: هادي رسول حسن المسعودي | زهير محمد علي المنگوشي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Karbala
First pages:

دراسة انتشار الاصابة بطفيلي الاميبا الحالة للنسيج Entamoeba histolytica وتحديد تركيز بروتين الصدمة الحرارية HSP70 وبعض المعايير الدمية لدى الاطفال المصابين في قضاء الناصرية / محافظة ذي قار العراق == Study Prevalence the Infection with Parasite Entamoeba histolytica and Determine Concentration of Heat Shock Protein HSP70 and Some Blood Parameters in Infected Children at the District of Nasiriyah-Province of DhiQar - Ira

Author name: سامي نجف بخور الياسري
Supervisor name: فاضل عباس منشد العبادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

الوبائية المصلية لمرضى داء المقوسات الكوندية في مرضى غسل الكلى (الديلزة) في بعض مستشفيات بغداد == The Seroepidemiology Of Toxoplasmosis In Hemodialysis Patients In Some Baghdad Hospitals

Author name: ايسر عماد عبد العزيز
Supervisor name: خوله حوري زغير
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

التحري عن الجينB1 في دم النساء الحوامل والمجهضات المصابات بطفيلي المقوسات الكونديه == Detection Of B1 Gene From Blood Of Pregnant And Abortive Women Infected With Toxoplasma Gondii

Author name: خوله حوري زغير الراوي
Supervisor name: سهيله حياوي محمود | هيفاء هادي حساني
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

التحري عن داء اللشمانيا الجلدية Cutaneous Leishmaniasis ومعرفة دور بروتين الصدمة الحرارية في الاستجابة المناعية في محافظة ذي قار HSP70 == Investigate of Cutaneous Leishmaniasis and knowledge of the role heat shock protein HSP70 in the immune response in the province of Thi Qar

Author name: نبیل عبد الجبار عبد الكاظم الموسوي
Supervisor name: فاضل عباس منشد العبادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Diyala
First pages:

دراسة تاثير بعض المستخلصات النباتية في طفيلي الزحار الاميبي Entamoeba histolytica داخل الجسم الحي == Study of the effect of Some Plant Extracts on Amoebic Dysentery Parasite Entamoeba histolytica In Vivo

Author name: غصون عادل عبد الحسين
Supervisor name: وفاء الحــــسني
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:

دراسة وبائية حيوية حول اصابة المضائف النهائية والوسطية بطفيلي المتورقة العملاقة Fasciola gigantica == Epidemiobiological biological study of Fasciola gigantica infection

Author name: مؤيد مجبل عبيد الجبوري
Supervisor name: صبيح هليل المياح | هادي رسول حسن
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Karbala
First pages:

The Effect Of Some Plants Extracts And Drugs On Immobilized Cysteine Proteinase Of Entamoeba Histolytica Isolated From Diarrhea In Al - Exandaria Districts - Babylon Province

Author name: هيام خالص عنفوص المسعودي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

دراسة بعض المعايير الكيموحيوية والفسلجية في النساء المصابات بداء المقوسات الكوندية Toxoplasmosis في محافظة النجف الاشرف == The Study of Some Biochemical and Physiological Parameter in Women Infected With Toxoplasmosis in Al-Najaf Al-Ashraf Governorate

Author name: سناء محمد علي حاتم الجلال
Supervisor name: هيثم محمد حمادي العوادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Najaf
First pages:

دراسة فسيولوجية وكيموحيوية للاطفال المصابين بطفيلي الجيارديا لامبليا Giardia lamblia وطفيلي الاميبا الحالة للنسج Entamoeba histolytica في كربلاء المقدسة == Physiological and Biochemical study of Children infected with Giardia lamblia and Entamoeba histolytica parasites in Holy Karbala

Author name: زينب عبد محسن الحبوبي
Supervisor name: ماهر علي القريشي | عبد الكريم عليوي جاسم
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Babylon
First pages:

دراسة وبائية لطفيلي Giardia lamblia في محافظة بابل واختبار فعالية المستخلص المائي البارد والمسحوق الخام لقشور ثمار الرمان في القطط والجرذان المصابة تجريبيا بالطفيلي == Epidemiological study for Giardia lamblia parasite in Babylon province and test activity of cold aqueous extract and crude powder of pomegranate peals in experimentay infected cats

Author name: حوراء صباح مهدي الموسوي
Supervisor name: سعدي محمد هلال | ماهر علي القريشي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Babylon
First pages:

دراسة فسلجية وكيموحيوية للحمام المنزلي Columba livia المصاب بالدودة الشريطية Raillietina spp. وتاثير المستخلص المائي الحار للثوم في علاج المضيف == A Physiological and A biochemical study on Domestic Pigone Columba livia Infected with the Cestoda Raillietina spp and the Efficacy of Garlic Hot Water Extract on the Host Treatment

Author name: اسراء جاسم حمود الحلو
Supervisor name: هيثم محمد حمادي العوادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Najaf
First pages:

عزل وتشخيص اميبا fowleri Naegleria حرة المعيشة ودراسة تاثيرها المرضي في الفئران المختبرية سلالة Balb/c == Isolation and Diagnosis of the free-living amoeba Naegleria fowleri and study of its pathological effect on mice strain Balb/c

Author name: نور فاضل عبد العباس المالكي
Supervisor name: مسلم عبد الرحمن محمد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Basrah
First pages:

دراسة وبائيه ومناعية لداء الحمى السوداء في قرى اهوار جنوب العراق == Epidemiological And Immunological Study On Visceral Leishmaniasis In Marshlands Villages South Of Iraq

Author name: هند مهدي جار الله
Supervisor name: عبد الحسين حبش عواد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: English
University location: Basrah
First pages:

دراسة تاثير هرمون Testosterone على الاصابة بالطفيليات Echinococcus granulosus وFasciola gigantica وLeishmania donovani في الحيوانات المختبريه في الزجاج == The effects of Testosterone on Facsiola gigantica , Echinococcus granulosus and Leishmania donovani In Vivo and In Vitro of Experimental animals

Author name: مسلم عبد الرحمن محمد الطعمة
Supervisor name: عبد الحسين حبش عواد | عبد النبي هادي العيسى
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:

الديدان المتطفلة في بعض الطيور البرية في محافظة البصرة == Helminthes parasitized on some wild birds of Basrah province

Author name: سامية شمخي جبر
Supervisor name: بسم هاشم عبد الله
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Basrah
First pages:

دراسة انتشار طفيلي الابواغ الخبيئة Cryptosporidium في بعض مصادر المياه والخضروات والحيوانات في محافظة البصرة == The distribution of Cryptosporidium in Some Resources of Water, Vegetables and Animals in Basrah Province

Author name: سارة كامل ناصر
Supervisor name: عبد الحسين حبش عواد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Basrah
First pages:

دراسة وبائية لانتشار داء الليشمانيا الجلدية والاحشائية Visceral Leishmaniasis & Cutaneous في محافظة ذي قار واختبار كفاءة بعض المبيدات على الحشرة الناقلة == Epidemiological study for distribution of cutaneous & visceral Leishmaniasis in Thi-Qar province and testing the efficiency of some pestisides on the insect vector

Author name: علي مساهر عطشان
Supervisor name: صادق ثاجب علي | بسعاد عقرب العبودي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

دراسة تشخيصية لطفيلي المقوسة الكوندية Toxoplasma gondii وفايروس الحصبة الالمانية Virus Rubella في النساء المجهضات باستعمال تقنيتي الاليزا وتفاعل سلسلة البلمرة بالوقت الحقيقي في محافظة ذي قار - العراق == Diagnostic study of Toxoplasma gondii and Rubella Virus in aborted Women using ELISA and Real- Time Polymerase chain reaction in Thi-Qar province - Iraq

Author name: رند كامل عباس العبودي
Supervisor name: فاضل عباس منشد العبادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

الديدان المتطفلة على القناة الهضمية لطيري الكوشرة Netta rufina والحذف الشتوي Anas crecca == The parasitic helminths of the Digestive tract of the ducks Netta rufina and Anas crecca

Author name: حبيب وسيل كاظم شبر
Supervisor name: محمد كاظم محمد | نجم عبد الواحد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:

التغيرات الدموية والكيموحيوية لدى الاطفال المصابين بطفيلي اللشمانيا الاحشائية Leishmania donovani == The haematological and Biochemical changes in children infected with Leishmania donovani

Author name: عباس كاظم عبد علي المنصوري
Supervisor name: هادي مدلول الميالي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:

استخدام وسط In-Pouch TV culture system كطريقة للتحري عن طفيلي Trichomonas vaginalis في بغداد/ الكرخ == The Usage Of In - Pouch TV Culture System As A Method For Trichomonas Vaginalis Detection In Baghdad - AlKarkh

Author name: ايات محمد سبع
Supervisor name: بان نوري القاضي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

تاثير الاصابة بداء المقوسات الكوندية على مستوى بعض الهرمونات الجنسية الذكرية في عينات من المركز الوطني لنقل الدم/ بغداد == The Effect Of Toxoplasmosis On The Level Of Some Male Sex Hormones In Samples From National Blood Transfusion Center - Baghdad

Author name: سهاد حسن محمود الساعدي
Supervisor name: بان نوري القاضي | خولة حوري زغيرالراوي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

تاثير بعض مركبات الايض الثانوي المعزولة من الطحلب الاخضر ضد طفيليي Cladophora crispata (Kuetizings ,1843) Entamoeba histolytica(Schaudinn, 1903) في الفئران المختبرية نوع Giardia lamblia)Stiles, و) 1915 Balb/c سلالة Mus musculus (L, 1758) == Effect of Some Secondary Metabolic Compounds Isolated From Alga Cladophora crispate( Kuetizings ,1843) against Entamoeba histolytica(Schaudinn, 1903) and Giardia lamblia(Stiles,1915) Parasites in Laboratory mice Species Mus musculus (L, 1758) strain Balb

Author name: دلال فلاح محسن العقابي
Supervisor name: صبيح هليل المياح | احمد محسن عذبي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Basrah
First pages:

وبائية الدودة الشريطية القزمة Hymenolepis nana في محافظة بابل مع تقييم فعالية مستخلصات نبات العاقول Alhagi maurorum في اصابتها التجريبية على الفئران البيض == Effect of alcohol and hot aqueous extracts for Leaves of Adhatoda vasica and the fungus Beauveria bassiana on some biological aspects of Musca domestica L (Diptera : Muscidae)

Author name: نور صالح عباس الخفاجي
Supervisor name: عماد احمد محمود | حسام الدين عبد الله محمد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Babylon
First pages:

الاصابات الطفيلية في بعض اسماك عائلة الشبوطيات وعائلة البياح في نهر دجلة المار في مدينة تكريت == Parasitic infections of some cyprinid and mugilid fishes from Tigris river passing through Tikrit city

Author name: ميساء ابراهيم علي الجبوري
Supervisor name: فاطمة شهاب الناصري
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:

دراسة وبائية المقوسات الكوندية Toxoplasma gondii بين الازواج في مدينة تكريت ومحاولة تجريبية عن امكانية انتقال الخمج جنسيا بين الفئران من نوع Mus musculus == Epidemiological study of Toxoplasma gondii between couples in Tikrit city , and experimental trial about possibility of sexual transmission of infection in mice type Mus musculus

Author name: مقصود عادل محمود الدوري
Supervisor name: توفيق ابراهيم الالوسي | عبد الله حسين الجبوري
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:

تقييم فعالية حليب وادرار الابل في حيوية ونمو طفيلي اللشمانيا الاحشائية Leishmania donovani مع التحري عنه في الحشرة الناقلة == Evaluate the effectiveness of camel,s milk and urine in the vitality and growth of Leishmania donovani with investigating with him in insect vectors

Author name: مصطفى جواد كاظم الغزي
Supervisor name: صادق ثاجب الغزي | بسعاد عقرب العبودي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

بعض الجوانب الحياتية للاصابة بداء اللشمانيا الجلدية في مدينتي تكريت وسامراء == Some biological aspect of infection with cutaneous leishmaniasis

Author name: مروة جاسم محمد الرشيد
Supervisor name: عبد الله حسين الجبوري | فاطمة شهاب الناصري
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:

كشف المقوسة الكوندية في النساء المجهضات في مدينة اربيل بواسطة الطرق المصلية، الجزيئية وحقن الفئران == Detection Of Toxoplasma Gondii Of Aborted Women In Erbil City Serological, Molecular (PCR) And Mouse Inoculation

Author name: لوزان سلام حسن خوشناو
Supervisor name: شمال محمد امين عبد الله | عزيز خالد حميد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: English
University location: Salahaddin
First pages:

دراسة وبائية وتشخيصية لبعض طفيليات اسماك الكارب الاعتيادي Cyprinus carpio L. لنهر دجلة - مدينة الموصل - مع الاشارة الى التغيرات في الخمج لثلاث انواع من الشريطيات == Epidemiology and diagnostic study on parasites of the carp , Cyprinus carpio L. for Tigris river /Mosul city /with special reference to the variation of infection of three cestodes

Author name: لازم صبحي شيت النعيمي
Supervisor name: شهاب احمد محمد الجبوري | عبد الله حسين عبد الله الجبوري
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:

تشخيص الاصابة بالادوار البالغة واليرقية لطفيلي Fasciola gigantica في قواقع Lymnaea auricularia باستعمال تقنية تفاعل انزيم السلسلة المتبلمرة Polymerase Chain Reaction (PCR) وتاثير درجة الحرارة على تلك الاصابة == Detection of parasitic adult and larval infection of Fasciola gigantica in Lymnaea auricularia using Polymerase Chain Reaction (PCR) and the effects of temperature on that infection

Author name: فاطمة فوزي يونس الحبيب
Supervisor name: عبد الحسين حبش عواد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Basrah
First pages:

تشخيص طفيلي البويغات الخبيئة باستخدام اختبار الاليزا وصبغه كاربول فوكسين المحورة وتاثير مستخلصي نباتي الزعتر والرمان على الطفيلي

Author name: عهود مزاحم شاكر محمود الرفاعي
Supervisor name: توفيق ابراهيم الالوسي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:

دراسة تشخيصية وبائية لبعض انواع الجنس انتاميبا Entamoeba بتقنية ال PCR في تكريت == Diagnostic and Epidemiological study of some species of Genus Entamoeba by PCR technique in Tikrit

Author name: علي محمد عبد ناصر
Supervisor name: ابراهيم شعبان داوود | عقيل حسين العاصي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:

وبائية بعض الطفيليات المعوية بين الاطفال في بعض اقضية محافظة ديالى == Epidemiology of certain intestinal parasites infection in certain Diyala districts

Author name: عبد الستار منصور عبد الزهيري
Supervisor name: عبد الرزاق شفيق حسن | نغم ياسين البياتي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Diyala
First pages:

وبائية الطفيليات الفموية Entamoeba gingivalis وTrichomonas tenax في محافظة بابل مع دراسة كفاءة وفعالية مستخلصات نبات الياس Myrtus communis L. في الفئران البيض المخمجة تجريبيا == Epidemiology of oral parasites Entamoeba gingivalis and Trichomonas tenax in Babylon Province with study of activity and ability of Myrtus communis L. extracts in experimentally infected white mice

Author name: شمس حامد كاظم السلطاني
Supervisor name: ماهر علي القريشي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Babylon
First pages:

دراسة وبائية عن بعض الاوالي المعوية الممرضة للاشخاص في قضاء الطوز مع دراسة تاثير الخمج بالجيارديا على انسجة الاثني عشري في الفئران المختبرية (Balb - c) == Study of some Intestinal Protozoan Parasites among peoples in AL-Touz town with study of Giardiasis on infected tissue in laboratory mice

Author name: شجعان رضا حسن ولي البياتي
Supervisor name: عبد الله حسين عبد الله الجبوري | الهام عائد اسعد التكريتي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:

الديدان المتطفلة في بعض الطيور المائية في هور الحمار - محافظة ذي قار مع الاشارة لبعض الجوانب البيئية لطفيليات طيور ابي قردان == Parasitic Helminthes in Some Aquatic Birds in Al-Hammar Marsh of Thi-Qar Governorate with Reference to Some of Environmental Aspects of the Parasites Birds Bubulcus ibis

Author name: زينب علي حسين الكناني
Supervisor name: فاضل عباس منشد العبادي | صبيح هليل جايد المياح
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

وبائية وتشخيص داء الاكياس العدرية Hydatid Disease في الانسان والحيوانات المجترة في محافظة ذي قار == Epidemiology and Diagnosis of Hydatid Disease in Human and Ruminant animals in Thi-Qar governorate

Author name: زهراء سعدون هادي الغزي
Supervisor name: فاضل عباس منشد العبادي | عـــدنان عيسى البــــدران
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

دراسة تاثير تغير العوامل الفسلجية والكيموحيوية لطفيلي Aporina delafondi في الحمام الاليف المعرض للجوع == Effect of change in physiological Biochemical factors of parasite Aporina delafondi in the Pigeon which suffered from hunger

Author name: زهراء جليل كريم تويج
Supervisor name: هيثم محمد حمادي العوادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Najaf
First pages:

تنمية طفيلي اللشمانيا الحشوية باستخدام اوساط زرعية مختلفة == Cultivation of visceral leishmania parasite by using Different culture media

Author name: رياض حاتم حداوي
Supervisor name: هادي رسول حسن المسعودي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Higher Diploma
Language: Arabic
University location: Karbala
First pages:

دراسة مقارنة تاثير مستخلصات بعض نباتات العائلة الشفوية على هرموني البروجسترون والاستروجين وعلاقتهما بالاستجابة المناعية في اناث الارانب والفئران المخمجة بطفيلي المقوسة الكوندية Toxoplasma gondii اثناء فترة الحمل == Study compartive effect of extracts some Plants of Labiatae on hormones Progesterone,estrogen and relanship with immunorespons In infected femal rabbits and mice with Toxoplasma gondii in stage

Author name: رانية شاكر محمود السامرائي
Supervisor name: توفيق براهيم الالوسي | وهبي عبد القادر سلمان
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:

عزل وتشخيص المسببات المرضية الطفيلية في الصرصر الامريكي Periplaneta americana (Blattaria: Blattidae) المتواجدة في منازل ومستشفيات محافظة الديوانية == Isolation and diagnoses parasitic pathogens in Periplaneta americana (Blattaria: Blattidae) present in the houses and hospitals of Al-Diwaniya governorate

Author name: حسام سعيد عبد الحسين العارضي
Supervisor name: هادي مدلول الميالي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:

وبائية داء الدبوسيات Enterobiasis بين تلامذة المدارس الابتدائية في قضاء بعقوبة == Epidemiological study of Enterobiasis among Pupils of primary schools of Baquba Distric

Author name: جابر عودة كاظم
Supervisor name: نبيل عبد القادر مولود | ناظم غزال نعمان
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Diyala
First pages:

الديدان المتطفلة على بعض الطيور المائية القاطنة على ضفاف شط العرب شمال محافظة البصرة == Helminthes parasitized on some aquatic birds which occupied on Shatt AL-Arab coast north of Basrah Governorate

Author name: بيداء فرحان سوادي
Supervisor name: باسم هاشم عبد الله
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Basrah
First pages:

تاثير مستخلصات بعض الاعشاب الطبية على النمو والعمليات الحياتية لمجاميع طفيلية مرضية مختلفة في العراق == EFFECT OF SOME MEDICAL HERBES ON GROWTH AND METABOLISM OF DIFFERENT CLINICAL PARASITIC GROUPS IN IRAQ

Author name: بلسم قبيس سعيد الفهادي
Supervisor name: حسين فاضل حسن بيرقدار | حسين اسماعيل ارتين الخان
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Salahaddin
First pages:

التحري الجزيئي ومدى انتشار طفيلي Giardia lamblia في مدينة بعقوبة == Molecular Investigation and the Prevalence of Giardia lamblia in Baquba city

Author name: انتصار مهدي حمد الحسيني
Supervisor name: نغم ياسين كاظم البياتي | هادي رحمن رشيد الطائي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Diyala
First pages:

محاولة علاج الاكياس العدرية في الفئران البيض بطريقة التمنيع والمواد الكيمياوية == A trial treatment of hydatidosis in white mice by Immunization and chemicals

Author name: امنة عبد الجبار ابراهيم
Supervisor name: وحيدة رشيد علي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Baghdad
First pages:

الديدان المتطفلة في بعض الطيور المائية واهمية الحشرات في دورة حياة البعض منها في محافظة البصرة == Helminthes parasitized of some aquatic birds & the impotance of insects in the life cycle of some of them in Basrah province

Author name: التفات عامر عبد الله التميمي
Supervisor name: عبد الحسين حبش عواد | ضياء خليف كريم
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Basrah
First pages:

الاصابات الطفيلية في الفار المنزلي Mus musculus domesticus في محافظة الديوانية

Author name: اخلاص عباس مرهون الزبيدي
Supervisor name: نجم عبد الواحد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:

دراسة دمية وكيموحيوية ومناعية للمرضى بداء الاكياس المائية == HAEMATOLOGICAL , BIOCHEMICAL AND IMMUNOLOGICAL STUDY IN PATIENTS WITH HYDATID CYST

Author name: احلام جاسم طاهر
Supervisor name: احسان مهدي الصقر | نهلة غانم عبد المجيد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
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