Show: 25 50 75 100 Results

Search results: 25 out of 281

Anatomical , Histological And Radiological Study Of Trachea And Lungs In Domesticated Local

Author name: Al - Anbaki Ahmed Abbas Ali
General topic: Veterinary Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University of Baghdad - College Of Veterinary Medicine
University location: Baghdad
Key words:
  • Veterinary anatomy

Anatomical And Histological Study Of The Small Intestine In Male And Female Indigenous Ducks : Anas Platyrhynchos

Author name: Ghassan Attiya Dawood
General topic: Veterinary Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University of Baghdad - College Of Veterinary Medicine
University location: Baghdad
Key words:
  • Veterinary anatomy

Histological Study Of The Rabbit S Colon Structure Indifferent Ages

Author name: Saif Muhson Ahmed
General topic: Veterinary Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University of Baghdad - College Of Veterinary Medicine
University location: Baghdad
Key words:
  • Colon - Anatomy - Veterinary anatomy - Veterinary Histology

Anatomical And Histological Postnatal Developmental Study Of Pituitary Gland, Testes And Accessory Sex Glands In Rabbits Oryctolagus Cuniculus

Author name: Al - Saadi . Ammar Ismail Jabbar
General topic: Veterinary Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Doctorate
University: University of Baghdad - College Of Veterinary Medicine
University location: Baghdad
Key words:
  • Veterinary anatomy

Anatomical And Histological Study Of The Lower Respiratory System In The Local Cats Felis Catus Domesticus L

Author name: rabab abd alameer nassar
General topic: Veterinary Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University of Baghdad - College Of Veterinary Medicine
University location: Baghdad
Key words:
  • cats - anatomy

Histomorphological Study Of Hematopoiesis Stages In Bone Marrow Of Adult Male Albino Rat Rattus Norvegicus

Author name: Al - Ta ae . Muntadhur Muhammad Cani
General topic: Veterinary Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Doctorate
University: University of Baghdad - College Of Veterinary Medicine
University location: Baghdad
Key words:
  • Veterinary anatomy

Anatomical And Histological Studies On Male Accessory Sexcalands In Mice ( Mus Musculus ) Rats ( Rattus Norvegicus ) And Guinea Pigs ( Cavia Porcellus )

Author name: Raid Riyadh Muhtan
General topic: Veterinary Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University of Baghdad - College Of Veterinary Medicine
University location: Baghdad
Key words:
  • Veterinory anatomy

Anatomical , Histological And Biochemical Study Of Remaining K Idney Ofter Unilateral Nephrectomy In Young And Adult Indigenous Rabbits Oryctola Cuniculus

Author name: al - khuzae bassim abdullah jassim
General topic: Veterinary Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Doctorate
University: University of Baghdad - College Of Veterinary Medicine
University location: Baghdad
Key words:
  • veterinary anatomy

Antaomical And Histological Study Of The Ovary And Oviduct In Turkey Hen Meleagris Gallopavo

Author name: riyadh hameed nsaif
General topic: Veterinary Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University of Baghdad - College Of Veterinary Medicine
University location: Baghdad
Key words:
  • veterinary anatomy

ظهور مؤشرات الخلايا الجذعية في النسيج الطبيعي المجاور CD44, ALDH1A1 وCD166 لسرطان القولون والمستقيم في عينات من المرضى العراقيين == Expression of Stem Cell Markers CD44, ALDH1A1 and CD166 in Normal Tissue Adjacent to Colorectal Carcinoma in Samples of Iraqi Patients

Author name: نوال مصطفى عبد الله
Supervisor name: مصطفى محمد ابراهيم | باسم شهاب احمد
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:

اظهار تعبير المؤشرين في عينات من الغدة الدرقية الطبيعية مقارنة مع عينات من الغدد الدرقية المجوثرة == Expression ofHIF-1?&HIF-2?inTissuesof NormalThyroidComparedtoTissuesofThyroid Multinodular Goitres

Author name: محمد حسين عاصي
Supervisor name: سامية عباس عليوي | باسم شهاب احمد
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:

دراسة الاحياء الجزيئية لنسيج الثدي الطبيعي المحيط بمنطقة السرطان للنساء العراقيات عن طريق التهجين الموقعي للمؤشرات ERBB2, TOP2A and C-MYC وعلاقتها بوجود == MOLECULAR BIOLOGY OF NORMAL MAMMARY TISSUE SURROUNDING BREAST CA OF IRAQI WOMEN THROUGH GENETICALLY FLUERESCENT IN SITU HYBRIDIZATION OF ERBB2, TOP2A AND CMYC IN RELATION TO ER & PR

Author name: حمد فاخر حميد
Supervisor name: مصطفى محمد ابراهيم
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Doctorate
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:

دراسة التاثير السمي لكبريتات النحاس المائية للجهاز التناسلي الانثوي واعضاء اخرى (نسجيا وبايوكيميائيا) على اناث الارانب المحلية == Study the toxic effects of copper sulfate pentahydrate on the female reproductive system and other organs histologically and biochemically) in Iraqi local rabbits)

Author name: زينة عبد الحسين يوسف العضب
Supervisor name: مجدي فيصل مجيد
General topic: Veterinary Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University Of Basrah - College Of Veterinary Medicine
Language: English
University location: Basrah
First pages:

دراسة مظهريه - نسجيه وكيمونسجيه مقارنه بين الماشية و الجاموس المحلي في الغدد اللعابية الكبيرة == Comparative Histo - Morphological and Histochemical Study of the Major Salivary Glands Indigenous Cattle and Buffaloes

Author name: رنا عماد المشهداني
Supervisor name: عادل جبار حسين
General topic: Veterinary Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University Of Basrah - College Of Veterinary Medicine
Language: English
University location: Basrah
First pages:

دراسة شكليائية نسجية للجهاز التنفسي السفلي في ذكور الغزلان المحلية البالغة (Gazella subgutturosa) == Histomorphological study of lower respiratory tract in adult male indigenous Gazelle (Gazella subgutturosa)

Author name: يوسف رافع جمعة
Supervisor name: فرحان عودة ربيع
General topic: Veterinary Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University of Baghdad - College Of Veterinary Medicine
Language: English
University location: Baghdad
First pages:

استخدام الصبغة المناعية p16INK4a والتهجين الموقعي وتاثيرها على فحوصات عنق الرحم الصحيحة والملتبسة == The Use of p16INK4a and In Situ Hybridization and Their Impact on the Healthy and Equivocal Pap Smears of the Cervix

Author name: ثناء جميل الخشالي
Supervisor name: ندى صالح امين | هدى مهدي الخطيب
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Doctorate
University: University of Baghdad - Faculty Of Medicine
Language: English
University location: Baghdad
First pages:
Abstract: Throughout the past thirty years, the perception of cervicalcarcinoma has shifted from that of a mysteriously fatal disease, to one ofthe sexually transmitted, human papillomavirus (HPV) related infection.HPV DNA has been found in almost all cervical invasive and preinvasivecervical neoplasms.Conventional Pap smear, which was established in the early sixtiesreduced effectively the morbidity and mortality related to cervical cancer.Given the lower sensitivity of the Pap cytology test, new diagnosticparameters have been established. The histological features ofpreinvasive cervical neoplasia (CIN1, CIN2, and CIN3) are wellunderstood. However, misinterpretation of the morphological criteriacould lead to significant variability.The aim of this study is to identify women with equivocal and normalPap smear who are at risk for developing cervical cancer through the useof p16INK4a immunostaining and human papillomavirus (HPV) DNAtesting using in situ hybridization (ISH).Materials and MethodsIn order to identify human papillomavirus infection in minorcytological and histological abnormalities, biopsies collected from 60women 25 - 66 years of age with normal cervices and Pap smears (groupI), abnormal cervices and Pap smears (group II) and with cervicalcarcinoma (groupIII) referred to the Colposcopy Clinic in BaghdadTeaching Hospital - Medical City Complex, during the period from June2013 through July 2014. Cytology samples collected with ThinPrep forIVliquid base cytology (LBC), punch biopsy for histopathology formalinfixed and paraffin embedded. In situ hybridization (ISH) and p16INK4aimmunohistochemistry in addition to routine hematoxylin and eosin(H&E) stain were used to evaluate the histological specimens.ResultsThe age, age of marriage and parity of the three groups werestudied in relation to each of ISH and p16INK4a reactions. Agedistribution was highly significant among all age groups; in both ISH andp16 test results GI &GII (p=0.00), GI & GIII (p=0.00), GII &GIII(p=0.00) in both ISH and p16 test results. The age of marriage was notsignificant among the groups in both ISH and p16; GI & GII p (0.93), GI&GIII (0.12), GII &GIII (0.30). The effect of parity was insignificantamong all groups; GI & GII = (p = 0.66), GI & GIII (p = 0.82), GII &GIII (p = 0.79) in both ISH and p16 test resultsp16INK4a immunoreaction : Among group I there was a negativereaction in all the specimens collected. In specimens from group II, therewas a very mild or focal p16INK4a immunoreaction in one specimen(5%), a moderate immunoreactivity in 9 specimens (45%), and a highpositive reactivity in 10 specimens (50%) (p=0.000). Group III showedvery high immunoreaction in all the specimens collected.In situ hybridization (ISH) in group I was mild positive in 4specimens (20%) which showed diffuse signal pattern. A mild positivereaction with diffuse nuclear staining was noticed in 4 (20%) of thecervical specimens. Moderate positive reaction with diffuse staining ofthe nucleus was observed in 8 (40%) of the specimens. The remaining 8(40%) of the specimens showed both diffuse and punctate staining of theVnuclei. Among group III specimens, the nuclei in the epithelial cellsshowed both punctate and diffuse signal patterns.Chi square was done to compare results concerning ISH & variousgroups was highly significant (p= 0.00) ConclusionHPV infection is prevalent and can be missed by the conventional Pap smear. The severity of the disease was directly proportional with the age of women in this study. p16 overexpression was correlated with the positivity of ISH. This may support the use of either, or both, tests to improve the accuracy of cervical intraepithelial neoplasia (CIN) diagnosis, and help in the triage of women with equivocal lesions.

علاقه بعض الحالات المرضيه بالاختلافات التشريحيه للقنوات الصفراويه في العراقيين باستخدام الرنين المغناطيسي الراسم للقنوات الصفراء والبنكرياس == Association of Some Pathological Conditions with the Anatomical Variations of the Biliary System in Iraqi People Using Magnetic Resonance Cholangiopancreatography(MRCP)

Author name: احمد سعد محسن
Supervisor name: نوفل خضير ياس الحديثي
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University of Baghdad - Faculty Of Medicine
Language: English
University location: Baghdad
First pages:
Abstract: Anatomic variations of the bile ducts are important to identify beforehepatobiliary surgical procedures as inaccurate determination of existing biliaryanatomic variations may potentiate ligature or section of aberrant ducts, leadingto major complications such as leakage or atrophy of the residual liver.Moreover, abnormal anatomical pattern of the biliary tree may predispose toincreased incidence of certain pathologies like gallstones. Therefore, it isapparent that thorough preoperative knowledge and successful detection andrecognition of such anatomic variations can lead to decreased morbidity andmortality rates during hepatobiliary surgery. Although several methods, like CTor MR Cholangiopancreatograms, have become the modality of choice fornoninvasive evaluation of abnormalities of the biliary tract, they are notroutinely used in preoperative imaging evaluation of patients.Magnetic resonance cholangiopancreatography (MRCP) is a safe, non - invasivediagnostic imaging technique, with added value of imaging post processing,allows accurate identification of biliary anatomy.For this study, ―Yoshida classification‖ was used to classify the anatomicalvariations of the biliary tree by using MRCP, Yoshida divide these variationinto 7 types, Type 1 where the right posterior segmental duct (RPSD) unit withthe right anterior segmental duct (RASD) to form the right hepatic duct (RHD)which unite with the left hepatic duct (LHD) to form the common hepatic duct(CHD) which unite with the cystic duct to form the common bile duct. Type 2in which the RPSD,RASD and the LHD unite together in triple confluence toform the CHD. Type 3 in which the RPSD cross to the left side to unite with theLHD, in Type 4 the RPSD have a lower insertion into the CHD, in Type 5; thereis triple confluence of the RPSD, Left inferior segmental duct (LISD) and Leftsuperior segmental duct (LSSD), while the RASD join the formed RHD to formviiCHD, while in Type 6; the triple confluence is between RASD,RPSD,LSSD andthe LISD drain into the CHD, Finally; Type 7 resemble type 1 except that theLISD an inferior insertion into CHD.Aim of the study : 1. To determine the most common anatomical patterns of the biliary tree inpatients and define the incidence of each pattern and to relate thesedifferent anatomical patterns to the incidence of biliary pathology.2. To measure the length of different branches of the biliary tree and findthe range of length in each branch and to relate the length of differentbranches of the biliary tree to the incidence of biliary tree pathology.Patient and Method : This study was conducted in the X - ray Institute in Baghdad Medical City usingmagnetic resonance cholangiopancreatography (MRCP) to scan patients withrelated biliary pathology.108 patients were taken for the study and the datacollected over 8 months periods from 10th of September 2014 to 28th of April2015. Full history and examination were done to the patients with basicinvestigation. Patients prepared before the scan by fasting and simple sedation.The images were taken both in coronal and transverse section; the resulting scanwas printed on disk and interprets by microDICOM software which hasmultiple functions and facilities, the cases then categorized according to theanatomical pattern and according to the pathological finding, Finally; the resultwere analyzed statistically.Results : Typical biliary tree (Type I) anatomical pattern was found in 64.8%.Anatomicalvariation was found in 35.2% and the most common anatomical variation isviiiwhen the right anterior hepatic duct crosses to the other side to drains into theleft hepatic duct and it is called Type 3 and found in 20.4% of cases while eachof type 2 and 4 was found in 7.4% of cases. The length of different branches ofthe biliary tree was variable and each branch had a wide range of length whichwere being recorded and compared. Pathological changes were found in 90.7%of cases which were mainly Gallstones, strictures and tumors. Gallstones werethe most common pathological finding with incidence of 79.6% while strictures were found in 3.7% and tumors in 7.4%. Relation between the variant anatomical pattern and pathological finding was significant. Also relationbetween the change in the length of the left hepatic duct and incidence of three mentioned pathological finding was significant, and relation between the change of the cystic duct and incidence of tumors in the biliary regions was also significant.Conclusion : MRCP scan of patients with liver related symptoms is of high importance on both surgical and medical methods. It will show us the complete anatomical pattern of the biliary tree and if there are any pathological conditions, additionally; we can take measurement of the branches of the biliary tree. Define the anatomical pattern of the biliary tree can give us a clue about the risk of this patient to develop certain pathological conditions even if the scan was normal. So it is advisable to take MRCP to any patient with liver related problems who underwent other liver specific investigation like US for evaluation and further accurate identification of the biliary tree anatomical pattern and of any existing pathological conditions and also for estimation of developing other pathological conditions in the future.

تحديد وحساب الخلايا البلعميه المعلمة بمادة (CD68) في مشيمية النساء الحوامل طبيعيا والمصابات بارتفاع الضغط الحملي == CD68 - labelled macrophages localization and counting in placentas of normal and pre - eclamptic women

Author name: لينا علي حسين
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University of Baghdad - Faculty Of Medicine
Language: English
University location: Baghdad
First pages:
Abstract: الخلفيه : تتكون المشيمه خلال عمليه معقده ومنسقه بين الانسجه التابعه لبطانه رحم الحامل والانسجه الجنينيه المحيطه. يوجد في المشيمه عدد من الخلايا البلعميه (البالعه) والتي يبدا ظهورها تقريبا في اليوم العاشر من بدايه الحمل. تشمل الخلايا البلعميه المشيميه خلايا هوفباورالتي توجد ضمن الزغابات (جزء المشيمه من جهه الجنين) والخلايا البلعميه الساقطه التي تقع ضمن الساقط القاعدي (جزء المشيمه من جهه غشاء الرحم). تتبنى هذه الخلايا نمطا ظاهريا متخصصا يلعب دوررائيسيا في عمليه الترسيخ المشيمي والجنيني, اضافه الى دورها في تنظيم المناعه الذاتيه للجسم تحت عوامل بيئيه مختلفه. ان السلوك الشاذ لهذه الخلايا يمكن ان يؤثر على وظيفه الارومه المغذيه وعلى نمو المشيمه وربما يؤدي الى مجموعه متنوعه من نتائج الحمل السلبيه. تاثير كل من تعدد الولادات وارتفاع الضغط الحملي على وظائف واعداد الخلايا البلعميه المشيميه لايزال موضوع جدل حتى الان.الهدف من الدراسه : ١) تحديد اماكن وجود وحساب اعداد الخلايا البلعميه ضمن المشيمات التابعه لنساء حامل للمره الاولى ونساء متعدده الولادات في مجموعه السيطره(الحمل الطبيعي) ومجموعه (ارتفاع الضغط الحملي) باستخدام تقنيه الكيمياء النسيجيه المناعيه (CD68 الاجسام المضاده الاوليه), لمعرفه تاثير تعدد الولادات على اعداد هذه الخلايا في المشيمه.٢) دراسه تاثير تعدد الولادات على بعض المتغيرات العينيه والمجهريه ضمن المشيمات التابعه لنساء حامل للمره الاولى ومتعدده الولادات في مجموعه السيطره(الحمل الطبيعي) ومجموعه (ارتفاع الضغط الحملي).المرضى والطرق : تم جمع ثلاثون مشيمه مولوده لحوامل تتراوح اعمارهم بين (٢٧ - ٣٢) سنه. استخدمت ست مشيمات للدراسه التجريبيه الاوليه, في حين قسمت الاربع والعشرون الاخرى الى مجموعتين : مجموعه السيطره ومجموعه ارتفاع ضغط الحمل ثم تم تقسيم كل مجموعه وفقا لتعدد الولادات الى مجموعات فرعيه (حامل للمره الاولى ومتعدده الولادات), (ست مشيمات لكل مجموعه فرعيه).تم فحص المشيمات عينيا من حيث (الشكل, موقع ادخال او ادراج الحبل السري, المحيط, القطر, السمك المركزي). بعد تثبيت ومعالجه وتقطيع العينات النسيجيه , تم صبغها باستخدام صبغه (الهيماتوكسيلين والايوسين) لدراسه المتغيرات المجهريه التاليه : عدد كل من ( الزغابات, العقد الخلويه, الاوعيه الشعريه الجنينيه), بالاضافه الى استخدام تقنيه الكيمياء النسيجيه المناعيه (CD68 الاجسام المضاده الاوليه) لحساب اعداد الخلايا البلعميه الموجوده ضمن الزغابات الجنينيه وتلك الموجوده ضمن الساقط الرحمي للمشيمات, كما اشتملت الدراسه على اجراء التحليل الاحصائي.النتيجه : يتراوح شكل المشيمات لمجموعتي السيطره وارتفاع الضغط الحملي من الدائري الى البيضوي. نسبه الادراج المركزي للحبل السري ارتفعت في مجموعه السيطره وخصوصا في (متعدده الولادات), في حين لوحظ زياده الادراج الطرفي للحبل السري في مجموعه ارتفاع الضغط الحملي وخصوصا في (الحامل للمره الاولى). كان هنالك انخفاض ملحوظ في قطر المشيمه في مجموعه ارتفاع الضغط الحملي للحامل للمره الاولى وكان ذلك متوافقا مع تناقص محيط المشيمه لنفس المجموعه كما كان هنالك زياده ملحوظه في السمك المركزي لمجموعه ارتفاع الضغط الحملي. التحليل الاحصائي للفحص المجهري ,اظهر زياده في عدد الزغابات الجنينيه, العقد الخلويه, والاوعيه الشعريه الجنينيه التي توافق زياده اعدادها مع ارتفاع الضغط الحملي وزياده الانجابيه. الزياده في اعداد الاوعيه الشعريه الجنينيه كان ملحوظا في (الحامل للمره الاولى) لمجموعه ارتفاع الضغط الحملي. اظهرت دراسه الكيمياء النسيجيه المناعيه زياده ملحوظه في اعداد الخلايا البالعه ذات الخاصيه الايجابيه للجزئ المنشط للمستقبلات ((CD68 ضمن الزغابات والساقط الرحمي لمشيمات مجموعه ارتفاع الضغط الحملي كما لوحظ توطن الخلايا البالعه ضمن الزغابات الجنينيه بالقرب من جدار الاوعيه الشعريه والارومه الغاذيه الخلويه في مجموعه ارتفاع الضغط الحملي وخصوصا في (الحامل للمره الاولى). | Background : formation of placenta occurs through a complex and coordinated effort between the fetus’s extraembryonic tissues and the gravid endometrial tissues. Many macrophages are present in the placenta throughout pregnancy and they have been detected as early as day 10 of pregnancy. Placental macrophages include hofbauer cells of the fetal villi and decidual macrophages of the maternal decidua basalis. They adopt a specialized phenotype that may hold a key role in implantation, placentation and parturition in both regulating and executing the body's own immune response under various environmental factors. Aberrant behavior of these macrophages can affect trophoblast function and placental development and potentially can lead to a spectrum of adverse pregnancy outcomes. Yet, the population and functions of placental macrophages in women with different parity and women with preeclampsia remain ill defined and subject of controversy.Aim of study : - • Localize and count the number of hofbauer cells and decidual macrophages in placentas of primiparous and multiparous of control and preeclamptic groups by using an immunohistochemical marker CD68 to find if parity can influence the number of placental macrophages. • Studying the influence of parity on some macroscopical and microscopical variables in placentas of primiparous and multiparous women of control and preeclamptic groups.Patients and Methods : A total of 30 placentas were collected from delivered women who were aging between 27 - 32 years. Six of them were used for preliminary pilot study while twenty four one were used for experimental study. Experimental placentas were grouped into two major groups (normal and preeclamptic) and each group was further subdivided according to parity into primi and multi subgroups (6 placentas for each subgroup). The placentas were grossly examined for their (shape, site of insertion of umbilical cord, circumference, diameter and central thickness). Then tissue samples were fixed, processed, sectioned and stained by heamatoxylin and eosin stain to study the following microscopical variables : number of (villi, syncytial knots and fetal capillaries). Additionally, immunohistochemical technique (CD68 primary antibody) was used to count the number of placental macrophages at fetal villi and maternal decidua. Statistical analysis (SPSS version20) was used in this study. Result : Studying placentas had circular to oval shape. The percentage of central insertion of umbilical cord was increased in control group, mainly in multi one. While marginal insertion was increased mainly in primi preeclamptic. There was a significant reduction in diameter of placentas of preeclamptic group, mainly in primi and it was positively correlated with placental circumference. The placental thickness was significantly increased in preeclampsia.Statistical analysis for histological variables revealed an increased number of villi, syncytial knots and fetal capillaries with preeclampsia and parity. The number of fetal capillaries was significantly increased with preeclampsia (mainly in primi subgroup). Immunohistochemical study revealed a significant increase in number of CD68 positive fetal and decidual macrophages in preeclamptic subgroups. CD68 positive fetal macrophages were seen to localize near fetal vessel wall and near syncytium which were significantly increased in primi preeclamptic subgroup. Conclusions : There were definite changes and correlations between macroscopical and microscopical variables in placentas of primi and multi subgroups of normal and preeclamptic groups. Immunohistochemically, the number of CD68 positive fetal and decidual macrophages were significantly increased in preeclamptic group in which hofbauer cells were mainly increased in primi subgroup. This could be attributed to factors like hypoxia and immunological maladaptation. These factors can induce a recruitment of macrophages to express different functions depend upon their locations.

تتبع مسار الارومات العصبية من منطقة ما تحت البطين باتجاه البصلة الشمية في ادمغة الفئران البالغة == TRACING THE PATHWAY OF THE NEUROBLASTS FROM THE SUBVENTRICULAR ZONE TO THE OLFACTORY BULB IN THE ADULT MICE BRAINS

Author name: زينب زاهد سعدون
Supervisor name: هدى مهدي الخطيب
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University of Baghdad - Faculty Of Medicine
Language: English
University location: Baghdad
First pages:
Abstract: لقد تم العثور على خلايا جذعية عصبية فريدة من نوعها في المناطق الرئيسية لتكوين الخلايا العصبية في ادمغة البالغين،سميت هذه المناطق بالاعشاش العصبية واكبر هذه المناطق هو منطقة ماتحت البطين للبطين الوحشي. يشار الى عملية تكوين الخلايا العصبية بانها العملية التي من خلالها تقوم الخلايا الجذعية العصبية، وذريتها من الارومات العصبية في توليد خلايا عصبية جديدة في الوضع الطبيعي والمرضي. ان دراسة هذه العملية وتتبع نتائجها مفيدة في وصف سلائف الخلايا العصبية وهجرتها خلال مساحات مقيدة في ادمغة الثدييات البالغة.اهداف الدراسة التعرف على الارومات العصبية على طول جدار البطين الوحشي في منطقة ما تحت البطين في دماغ الفار البالغ وكذلك تتبع هذه الخلايا على طول مسار خاص من منطقة ماتحت البطين حتى البصلة الشمية يعرف هذا المسار بالتيار المهاجر المنقاري. تم ذلك عبر التصبيغ النسيجي العادي باستخدام الهيماتوكسيلين والايوسين(H وE) والتصبيغ المناعى النسيجي الكيميائي باستخدام الجسم المضاد antidoublecortin المتخصص بتحديد الارومات العصبية. المواد والطرائق تم تنفيذ الدراسة في مبنى الحيوان، كلية الطب / جامعة بغداد. من خلال جمع وتربية 36 من الفئران الاناث والذكورنوع (Micromys minutus)، اربعة منها حديثة ولادة استخدمت لعرض الشاهد الايجابي لعمل الجسم المضاد. ال32 الاخرى كانت بالغة (اكبر من 60يوم) استخدمت سبعة منها لاجراء الدراسة الاستطلاعية. ال25 الاخرى استخدمت لاجراء الدراسة الحقيقية حيت تم تثبيت ادمغتها من خلال ارواء مادة البارافورمالدهيد عن طريق القلب مع جهاز مضخة صغيرة تم تصنيعه محليا لهذا الغرض ثم الحصول على ادمغتهم مباشرة بعد ذلك. بعد تشريح الادمغة اكليليا وسهميا تم تثبيتها لمدة 20 ساعة في نفس المادة المثبتة المستخدمة في الارواء. تمت المعالجة بالتجفيف في درجات متزايدة من الكحول الاثيلي والتصفية في الكلوروفورم ثم الغمر في البارافين. تم الاجتزاء بواسطة مشراح، بعد ذلك تمت ازالة البارافين بواسطة الزايلين والاماهة بدرجات متناقصة من الكحول الاثيلي ثم التصبيغ بواسطة صبغة الهيماتوكسيلين والايوسين. للتصبيغ المناعى،تمت حضانة الاجسام المضادة الاولية antidoublecortin مع النسيج لمدة 2 ساعة وللاجسام المضادة الثانوية لمدة 1 ساعة بدرجة حرارة 30°, ثم اضافة صبغة الداب كخطوة نهائية. وقد تم القيام برؤية النسيج المصبوغ من خلال المجهر الضوئي النتائج في المستوى الاكليلي المار بالبطين الوحشي بمسافة 1 ملم امام اليافوخ الامامي، تم تحديد منطقة ماتحت البطين. بالتصبيغ النسيجي العادي الهيماتوكسيلين والايوسين (H and E) ظهرت هذه المنطقة كتجمعات خلايا مجاورة لخلايا البطانة العصبية. مع التصبيغ المناعي الكيمائي، استطعنا ان نثبت بان كتل خلايا من هذه التجمعات تمثل الارومات العصبية وذلك لاخذها الصبغة الايجابية للاجسام المضادة antidoublecortin. على الرغم من هذا فان مجموعة من الخلايا المحيطة بكتل الارومات لم تعطي التصبيغ الايجابي لهذا الجسم المضاد لكنها ظهرت بوضوح وهي تنتشر بين كتل الارومات العصبية. جرى تتبع هذه الارومات سهميا من منطقة ما تحت البطين. وقد وجد انها تشارك في مسارملتزم يدعى التيار المهاجر المنقاري ، يبدا من الطرف الامامي للبطين الجانبي وينتهي في منتصف الفص الشمي . الارومات في التيار المهاجر المنقاري تصطف عرضيا بشكل موازي لسطح الدماغ وتدعم بعضها البعض لتشكيل ما يبدو انه شريط سلسلي من خلايا ممدودة الشكل ذات انوية مغزلية غامقة محاطة بشكل وثيق من قبل خلايا اخرى متعددة الاشكال لم تاخذ صبغة الجسم المضاد antidoublecortin. وقد تم ملاحظة شكل مميز وواضح للسلسلة ابتداءا من منبعها وحتى نقطة انتهائها. تشكل السلسلة تيار منحني بشكل حرف S ينقسم الى اربعة اجزاء مميزة : القمع والطرف العمودي والمرفق والطرف الافقي. علاوة على ذلك , تتخذ الارومات العصبية معالم شكلية مختلفة على طول الاجزاء الاربعة للتيار حيث يتغير شكلها من الشكل الممدود والانوية المغزلية في القمع والطرف العمودي الى الشكل البيضوي الاقل انتظاما في المرفق ثم الشكل الكروي في الطرف الافقي . غير التيار المهاجر المنقاري وضع الهجرة بالقرب من قطبه المنقاري من الوضع العرضي الى الوضع الشعاعي الذي تدخل به الارومات العصبية داخل البصلة الشمية. بالاضافة الى ذلك خضعت الارومات العصبية في التيار المهاجر المنقاري للانقسام الخيطي مما ادى الى زيادة عددها بالقرب من نهاية التيارالمهاجر | adult brain, these regions called neurogenic niches, the larger of which is the subventricular zone (SVZ) of the lateral ventricle. Adult neurogenesis is referred to the process in which neuronal stem cells, and their progeny the neuroblasts; generate new neurons in physiological and pathologic conditions. The study of this process and the tracing of its consequences are beneficial in describing the precursors of neurons and its migration through restricted territory in the adult mammalians brains. Aims of studyTo identify the neuroblasts, along the wall of the lateral ventricle, the SVZ, in the adult mouse brain and to trace them from the SVZ to the OB along the special pathway, the Rostral Migratory Stream (RMS), using routine stains the Hematoxylin and Eosin (H and E) and immunohistochemical staining with antidoublecortin antibody, the specific marker of the neuroblasts. Materials and methodsThe study have been executed at the animal house of College of Medicine \ Baghdad University; by collecting and breeding 36 male and female mice (Micromys minutus), 4 of them were neonates used for demonstrating positive control for the antibody, and the other 32 were adults (< 60 days old), seven of them were used for pilot study. The other 25 were used for proper study. They were perfused intracardially by paraformaldehyde solution with a mini - pump apparatus that has been constructed locally for this purpose then harvesting their brains immediately. After dissecting the brains coronally or sagittaly they were fixed for 20 hours in the same fixative used for the perfusion. Processing had been done by dehydrating in ascended grades of ethanol alcohol and clearing in chloroform then embedding in paraffin. Sectioning had been done with microtome; deparaffinization by xylene and rehydration with descended grades of ethanol alcohol then staining by H and E. For the immunohistochemical staining, the primary antibody “antidoublecortin antibody” was diluted to (1/1000) and incubated with the tissue for 2 hours at 30 C°. Incubation with the secondary antibody lasted for 1 hour. Application of DAB was the final step. Visualization had been done with a light microscope.Results At the coronal plane through the lateral ventricle "1 mm anterior to bregma", identification of the SVZ had been done. By the ordinary staining "H and E", the zone appeared as an aggregate of cells next to the ependymal layer. With the immunohistochemical staining, clusters of cells were proved to be the neuroblasts by staining positive for the antidoublecortin antibody; though groups of surrounding cells did not express the signal of this marker but apparently interspersed among the clusters of the neuroblasts. The neuroblasts were traced sagittaly from the SVZ and they have been found to be engaged in a committed pathway called RMS, began from the anterior tip of the lateral ventricle and ended at the core of the OB. The neuroblasts in the RMS oriented tangentially parallel to the brain surface and scaffolded each other forming what seemed to be a chain - like strip of cells which were elongated with dark spindle shaped nuclei and surrounded intimately by another cells, polymorphic in shape and did not take the signal of the antidoublecortin antibody. Distinct morphology of the chain had been encountered grossly from its emergence site till its termination point. It was forming a sigmoidal shape stream that could be divided as a whole into four distinct parts; infundibulum, vertical limb, elbow and horizontal limb. Furthermore, the neuroblasts took different morphological features along the stream. They changed from spindle shaped - nuclei cells in the infundibulum and the vertical limb to oval or irregular - shaped nuclei cells in the elbow and to more spherical - shaped nuclei in the horizontal limb. The RMS might change the mode of the migration near its rostral pole from the tangential parallel mode to the radial scattered mode by which the neuroblasts entered the olfactory bulb. In addition, the neuroblasts in the RMS revealed mitotic activity and increase their number near the termination of the stream.

دراسة كيميانسيجية مناعية لمعرفة التغيير العددي والسلوكي للخلايا الجذعية في المعدة لبعض الحالات المرضية == Immunohistochemical study to identify the changes in number and behavior of gastric stem cells in some pathological conditions

Author name: علي عبد المحسن سلمان الشافعي
Supervisor name: نوفل خضير ياس الحديثي
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University of Baghdad - Faculty Of Medicine
Language: English
University location: Baghdad
First pages:
Abstract: الخلايا الجذعية هي خلايا في اجسامنا لها القابلية على التكون الذاتي والتميز الى انواع مختلفة من الخلايا وذلك لبناءالانسجة والاعضاء. في العلم مازال هناك تحدي لفهم كيف للبيضة المخصبة ان تتطور الى طبقات جرثومية وانواع متددة من الخلايا والتي تتطور لاحقا الى انسجة متعددة واعضاء ذات وظائف حيوية مختلفة. هناك انواع عديدة من خلايا الجذعية, والخلايا الجذعية المعدية هي واحدة منها والتي هي خلية غير ناضجة شكليا ومسؤلة لتوليد انواع الخلايا الرئيسية في الغدة المعدية ويمكن ان يكون لها دور في تكوين سرطان المعدة. يظن ان هذه الخلايا تحافظ على مجاميع صغيرة وثابتة من الخلايا الجذعية التعددة القدرات (multipotent stem cells) في المعدة من خلال الانقسام المتماثل الغير متكرر. الخلايا الوليدة تتكون بواسطة الانقسام المتكرر الغير متماثل للخلايا الجذعية المتعددة القدرات لتعطي الخلايا الجذعية التي تتميز وتكون نسل مختلف انواع الخلايا الطلائية عندما تهاجر باتجاهين متعاكسين, اما عند تجويف الغدة العلوي او الى الغدة نفسها. تهدف الدراسة الى تحديد اعداد الخلايا الجذعية المعدية في حالة التهاب المعدة والقرحة والحالات ما قبل السرطانية ووسرطان المعدة ومقارنتها بالحالات الطبيعية وايضا دراسة تاثير الجنس والعمر على هذه الاعداد.تمت الدراسة باخذ 72 خزعة من المرضى الذين يجرى لهم ناظور للمعدة والامعاء في وحدة الناظور لمستشفى بغداد التعليمي لفترة 5 اشهر. وقد تم تقسيم المرضى حسب التشخيص النسيجي الى 5 مجاميع (طبيعي والتهاب المعدة وتقرح المعدة وحالات ما قبل السرطانية والحلات السرطانية). تم معالجة العينات وصبغت بمادتي الهيماتوكسلين والايوسين لغرض التشخيص النسيجي ومجموعة اخرى من السلايدات اعدت لغرض الدراسة الكيميانسيجية المناعية بواسطة الاجسام المضادة لل (lgr5).وقد اظهرت النتائج ان التعبير النسيجي الايجابي للخلايا الجذعية بواسطة ال(lgr5) يزداد مع الحالة المرضية. وان هذد الزيادة تصل الى الذروة في الحالات ما قبل السرطانية وتنخفض قليلا في حالة سرطان المعدة. وكان المتوسط العددي للخلايا الجذعية في معدة الانسان الطبيعي 7خلايا وفي حالة التهاب المعدة 18 خلية وفي قرحة المعدة 25 خلية وفي الحالا ما قبل السرطانية 35خلية واخيرا 28 خلية في حالات سرطان المعدة. ووجد ان اعلى معدل للخلايا الجذعية في معدة الانسان الطبيعي يكون في الاعمار المتوسطة (20 - 40 سنة) وينخفض مع تقدم العمر.وبالاستنتاج وجد ان الخلايا الجذعية المعدية تزداد مع المرض وان هذه الزيادة مرتبطة بشدة المرض نفسه من جهة (كما في التهاب المعدة وقرحة المعدة) واما اذا كانت الخلايا الجذعية تشارك في تكوين السرطان في المعدة من جهة اخرى (كما في الحالات ما قبل السرطانية وسرطان المعدة) | Background : Stem cells are a group of cells in our bodies, with the capacity to self - renew and differentiate to various types of cells, thus to construct tissues and organs. In science, it is still a challenge to understand how a fertilized egg develops germ layers and various types of cells, which further develop into multiple tissues and organs with different biological functions. There are many types of stem cells, gastric stem cells is one of these types, which are morphologically immature cell responsible for generating the major cell types of gastric gland and it may have a role in development of gastric cancer. These cells are thought to maintain a small, steady - state population of multipotent stem cells in the gastric unit through infrequent symmetric division. Daughter cells generated by much more frequent asymmetric division of these multipotent stem cells subsequently exit the stem cell niche and differentiate to generate the various epithelial cell lineages as they migrate bidirectionally toward the pit or gland.Aim of study : 1) Identify the gastric stem cells population in gastritis, gastric ulcer, premalignant conditions and gastric cancer and comparing them to normal mucosal stem cells. 2) Study the effect of sex and age on this population. Patients and methods : 72 biopsies were taken from patients underwent OGD in the endoscopic unit of Baghdad Teaching Hospital over a period of 5 months. Patients were divided histopathologically into 5 groups (normal, gastritis, gastric ulcer, premalignant conditions and gastric cancer). Specimens were processed & slides stained with H & E were examined histopathologically, a second set of slides were prepared for immunohistochemial study with Leucine - rich Repeat Containing G - Protein Coupled Receptor 5 (Lgr5) antibody.Results : The results showed that the expression of lgr5+ stem cells was increased with pathology. This increase reaches its maximum in premalignant conditions and slightly declines in gastric cancer. The average number of stem cells pre high power field in normal stomach was 7 cells, in gastritis was 18 cells /HPF, in gastric ulcer was 25 cells /HPF, in premalignant conditions was 35 cells /HPF and in gastric cancer was 28 cells /HPF. The highest number of stem cells in normal stomach was in middle age group and declines with aging.Conclusions : the number of gastric stem cells increase with pathology and this increase is related to aggressiveness of the disease on one hand (gastritis & gastric ulcer) & on whether stem cells participate in tumor pathogenesis on the other hand (premalignant conditions & gastric cancer).

تاثير الفيتامينات B1,B6 and B12) على الاعتلال العصبي المحيطي : دراسة نسيجية، نسيجية كيميائية وكيميا نسيجية مناعية == The effect of vitamins B1, B6 and B12 on peripheral neuropathy , Histological , Histochemical and Immunohistological studies

Author name: صالح مهدي علي
Supervisor name: معن حمد الخالصي
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Doctorate
University: University of Baghdad - Faculty Of Medicine
Language: English
University location: Baghdad
First pages:
Abstract: Peripheral neuropathy is a nerve injury process by disease or so on leading to damage of peripheral nervous system . Diabetes mellitus and compression are most common causes of peripheral neuropathy. Vitamins (B1,B6 and B12) commonly used for treatment of peripheral neuropathy. The objective of this study is to compare the effect of vitamins (B1,B6 and B12) on nerve regeneration and recovery after induced sciatic nerve crush injury in experimental animal model.Seventy five male Swiss albino rats were divided randomly into five groups and each group consisted of 15 animals. Group A( which considered as control positive(with crush injury) received normal saline intramuscularly for 45days. Group B received vitamin B1 , group C received vitamin B6 , group D received vitamin B12 and group E which considered as control negative(no crush injury and no treatment) . All animals were injected vitamins intramuscularly for 45days. Tail flick test time was reduced in rats treated with vitamin B12 after crush injury to the Sciatic nerve. Mid thigh circumference (MTC) was significantly increase in B12 treated group after crush injury to the Sciatic nerve. Data analysis was performed by SPSS 20 software using descriptive statistics.IVHistological examination of rats sciatic nerves stain by H&E on day 15 showing degenerative nerve fibers with Schwann cell infiltration in all groups but it is less prominent in group D. On day 45 histological examination of rats gastrocnemius muscles exhibited separation of bundles muscle fibers by connective tissue, variation of fibers in size and shape with atrophy of muscle bundles in all groups .While these were less prominent in rats treated with B12.Van Giesons staining of rat sciatic nerves on day 45 after crush injury. In all groups experimental and control (B,C,D,A) showing degenerative nerve fibers in different areas but less in group D and better organized sciatic nerve fibers in group D.Finally, Immunohistochemical study of (anti S100 protein) of rat sciatic nerves on day 45showed strong activity for those rats treated with vitamin B12 while rats treated with vitamin B1 and B6 exhibited moderate and weak activities respectively .In conclusion vitamin B12 promote peripheral nerve regeneration more than vitamin B1and vitamin B6.

تاثيرات عقاري المتفورمين والاناستروزول على شكل ووظيفة المبيض في بمتلازمة تكيس المبايض في الجرذان : دراسة شكلية قياسية، نسيجية، وكيميانسيجية == The Effects of Metformin and Anastrozole on the Morphology and Function of the Ovary in Polycystic Ovarian Syndrome Rat Model, Morphometrical, Histological, and Immunoistochemical Study

Author name: علي محسن عبد الامير
Supervisor name: معن حمد الخالصي
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Doctorate
University: University of Baghdad - Faculty Of Medicine
Language: English
University location: Baghdad
First pages:

الجملة الوعائية المجهرية لمخيخ الجرذ == Microvasculature of the Rat Cerebellum

Author name: حسنين عبد الجبار العبيدي
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University of Baghdad - Faculty Of Medicine
Language: English
University location: Baghdad
First pages:
Abstract: In spite of its various anatomical, functional, and phylogenetic divisions, the rat cerebellum remains constant throughout, in its cellular and laminar organization. The question remains whether the microvascular supply of the cerebellum is quantitatively homogenous or not.This study aims to investigate quantitatively whether the homogeneity in cerebellar cellular architecture is reflected on the functional vascular bed in different regions of the cerebellum and to provide reliable morphometric parameters that can be used for comparative purposes with other regions in the central nervous system.A sample of 20 adult male albino rats was used. Fresh frozen 20 μm thick sections in the sagittal plane were stained for microvascular alkaline phosphatase. Image analysis software (Global Lab Image), was used to measure circumscribed areas for the length and number density of their contained alkaline phosphatase positive microvascular profiles. Microvascular densities in the cortical layers of the cerebellum as a whole was (76.72±30.59 count/mm2) and (231.45±82.14 mm/mm3). There was a higher microvascular density in the granular layer in comparison to the molecular layer. This was significantly higher than the densities of the white matter core excluding the deep cerebellar nuclei. The densities in the deep cerebellar nuclei were (122.86±20.38 count/mm2) and (328.22±33.45 mm/mm3). Length density did not show obvious heterogeneity in the cortical layers between different divisions of the cerebellum. Number density was significantly higher within the cortical layers of the hemisphere when compared to the vermis. A comprehensive guide for identifying rat cerebellar regions was put foreword. This guide can be useful for identifying rat cerebellum sagittal sections regardless of the level of the section or the staining method used without the need to follow an entire series of sections.The differences in microvascularity on the laminar level was correlated with the functional necessity of neurons and synapses. Neurovascular coupling appeared in its best manifestation in the deep cerebellar nuclei where it was clear that dense microvascularity was concomitant with a densely synaptic and neuron - poor region.The cerebellar cortex as a whole, was homogenous in its microvascular length density in whichever division, anatomical, functional or phylogenetic.The predilection towards heterogeneity between vermian and hemispheric cortices as far as number density is concerned can be attributed to the complexity of branching for a given length of vessels.A combination of two morphometric parameters (microvascular length and number density) is a reasonable choice to explore the microvascular network in terms of functional needs and spatial composition.

دراسة كمية للخواص الكيميانسيجية والشكلية لعضلات الطرف الخلفي للجرذ == Quantitative Study of the Histochemical and Morphometric Characteristics of the Rat Muscles of the Hind Limb

Author name: مثنى عبد الامير عبد اللطيف
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University of Baghdad - Faculty Of Medicine
Language: English
University location: Baghdad
First pages:

دراسة لمقارنة مستقبلات للهرمون المحرر لموجهة القند لقناة فالوب بواسطة تقنية المناعة النسيجية الكيميائية في النساء اللائي لديهن حمل خارج الرحم, او عملية استئصال الرحم او ربط القناة == Comparative Study of Gonadotropin - releasing Hormone Receptor in Fallopian Tube by Immunohistochemistry among Women with Ectopic Pregnancy, Hysterectomy and Tubal Ligation

Author name: هالة ناظم كاظم مهدي
Supervisor name: ايمان علي هاشم | سعد عبد الباقي عبد الله
General topic: Medicine
Specific topic: Anatomy and Histology and Embryos
Degree: Master
University: University Of Basrah - Faculty Of Medicine
Language: English
University location: Basrah
First pages:
Abstract: الهدف : اجريت هذة الدراسة للمقارنة في مستوى مستقبلات للهرمون المحرر لموجهة القند بواسطة تقنية المناعة النسيجية الكيميائية في قناة فالوب بين النساء اللاتي اجريت لهن عملية ربط الانابيب عند الولادة او الحمل خارج الرحم او عملية استئصال الرحم خلال الطور الافرازي/ اللوتني من دورة الطمث.المرضى والمواد : شملت الدراسة على ثلاثة مجاميع من النساء المريضات. ضمنت المجموعة الاولى على 39 امراة تتراوح اعمارهن بين 15 - 45 سنة لديهن تاريخ سابق للحمل المنتبذ (خارج الرحم) واجريت لهن عملية طارئة. اما المجموعة الثانية ضمت 40 امراه تتراوح اعمارهن بين 25 - 45 سنة اجريت لهن عملية استئصال الرحم الاختياري نتيجة لاسباب نسائية حميدة مختلفة وشملت الاورام الليفية المتعددة ، والعضال الغدي ،او النزيف المهبلي الذي لم يستجب للمعالجة الطبية. هذا بالاضافة الى شمول 40 امراه في اما المجموعة الثالثة فقد شملت 40 امراة تراوحت اعمارهن بين 26 - 45 سنة اللواتي خضعن الى عمليات قيصرية مع ربط القناة (البوق)الثنائي عند اكتمال الحمل. شملت معايير الاقصاء المريضات اللواتي يعانين من امراض الحوض الالتهابية والانتباذ البطاني الرحمي وكذلك اللواتي يستعملن الهرمون المضاهي لهرمون اللوتنة . اجريت هذه الدراسة خلال الفترة من ايلول 2014 وحزيران 2015 في مستشفى الولادة والطفل في البصرة. تمت ازالة قنوات فالوب من المريضات اللواتي اجريت لهن عملية ازالة جراحية لهذه القنوات في المجاميع الثلاث. وقد تم حفظ قنوات فالوب في 10% فورمالين وارسلت الى مختبر الامراض في مستشفى الصدر التعليمي لغرض الفحص النسيجي المرضي (باستعمال طريقة التلوين بالهيماتوكسلين والايوسين ) ولغرض التقصي المناعي النسيجي الكيميائي.النتائج : تبين في الفحص النسيجي المرضي كان هناك العديد من الزغابات المشيماتية الشاحبة في تجويف قناة فالوب. وهناك ايضا طبقات من الارومات الغازية تمتد بشكل حر داخل التجويف . ودل الراسب البني في السايتوبلازم خلايا قناة فالوب على التلوين بواسطة الاضداد الاولية ، ولايوجد تلوين في العينات السالبة باستعمال الفحص المناعي النسيجي الكيميائي . وهناك اختلاف في توزيع وجود مستقبلات للهرمون المحرر لموجهة القند بين النساء ذوات الحمل المنتبذ (58,9%) واللاتي استئصلت ارحامهن (82%) وفي 10% فقط بين النساء اللاتي اجري لهن ربط قناة فالوب عند الولادة وكان ذلك باستعمال تقنية المناعة النسيجية الكيميائيه لكن ذلك كان قريبا من الاعتداد الاحصائي ( الاحتمال =0,069) . بينما كان التوزيع السلبي لمستقبلات الهرمون المحرر لموجهة القند اعلى في النساء اللاتي اجري لهن ربط القناة (90%) مقارنة مع النساء اللاتي لديهن حمل منتبذ (41,1%) او النساء اللاتي اجري لهن استئصال الرحم (18%). ان مستقبل الهرمون المحرر لموجهة القند المتفاعل مناعيا تم تحديده في عينات قناة فالوب من مريضات الحمل المنتبذ في الغشاء المخاطي للقناة فقط (5 من 39) او في الزغابات المشيماتية فقط (6 من39) او في كليهما (13 من 39) ، وكانت الفروقات غير معتدة احصائيا ( الاحتمال اكبر من 0,85).الخلاصة : لقد تبين مما تقدم بان مستقبل الهرمون المحرر لمواجهة القند يملك دور حيوي في بداية الحمل لللاخصاب وغرس الجنين. | Objective : This is a comparative study to detect gonadotropin - releasing hormone receptor expression by immunohistochemical technique in the human fallopian tubes belong to women with ectopic pregnancy, women subjected to tubal ligation at term pregnancy with history of normal pregnancy and hysterectomized women.Patients and Methods : The study involved 3 groups of patients, the first group was involved 39 females ( their ages ranged from 15 - 45 years) with history of ectopic pregnancy who underwent emergency operation, the second group was included 40 (their ages ranged from 25 - 45 years) women who were operated on for elective hysterectomy due to various benign gynecological reasons, like multiple uterine fibroid, adenomyosis and uterine bleeding not responding to medical treatment. The third group was involved 40 women (their ages ranged from 26 - 45 years) subjected to Cesarean section with bilateral tubal ligation at term pregnancy were also included in the study. The exclusion criteria included patients with pelvic inflammatory disease, endometriosis and luteinizing - hormone - releasing hormone analogue users. The study was carried out during the period from September 2014 till June 2015 at Basra Maternity and Childhood Hospital. Fallopian tubes were removed and collected from all the 3 groups patients. Fallopian tubes were preserved in 10% formalin and were taken to the Pathology Laboratory at Al - Saddar Teaching Hospital for the purpose of conventional histopathology i.e, Hematoxyline eosin staining method and immunohistochemical study for gonadotropin - releasing hormone receptor.Results : Histopathological examination of the tissue of fallopian tube, indicates that there are numerous pale chorionic villi in the lumen of the fallopian tube. A brown precipitate in the cytoplasm of the cells of the fallopian tube indicated positive staining by primary antibody while no staining was detected in negative samples by using immunohistochemical examination. There is a difference in the distribution positive cases of gonadotropin - releasing hormone receptor detection among ectopic pregnant women (58.9%), hysterectomized women (82%) and only (10%) among women with tubal ligation at term pregnancy in the fallopian tube by using immunohistochemical technique but statistically the results is marginally significant (P=0.069). The immunoreactive GnRH receptor was identified in the fallopian tube samples from patients with ectopic pregnancy, in the mucosa of the tube alone (5 out of 39) or in the chorionic villi only (6 out of 39) or both of them the mucosa as well as villi (13 out of 39 ). The differences were statistically insignificant (P>0.85). Conclusion : This study was demonstrating that GnRH which is produced by the human fallopian tube, has a vital role in fertilization, early embryonic development and implantation .
1 ... 5 6 7 8 9 ... 12