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تحسين اداء بكتريا Methylotrophs باستخدام نظام تعديل الجينات CRISPR / Cas9 للتكسير الحيوي لمركبات الهيدروكربون == Enhancing the performance of Methylotrophs bacteria by using CRISPR/Cas9 Gene Editing System for Biodegradation Hydrocarbon Compounds

Author name: انوار عبد الوهاب مكي

Supervisor name: اسعد محمد رضا الطائي | زينة وحيد عطوان

General topic: Biology

Specific topic: Microbiology

Degree: Doctorate

University: University Of Basrah - College Of Science - Department Of Biology

Language: English

University location: Basrah

First pages:

التخليق الحيوي لجسيمات الفضة واوكسيد النحاس النانوية بواسطة البكتيريا البيئية مع بعض التطبيقات الطبية == Biosynthesis of Ag and CuO nanoparticles using environmental bacteria with some medical applications

Author name: حسن غالي عبد الحسن

Supervisor name: وجدان حسين عبد الصاحب التميمي | رشيد رحيم حتيت

General topic: Biology

Specific topic: Microbiology

Degree: Doctorate

University: University Of Basrah - College Of Science - Department Of Biology

Language: English

University location: Basrah

First pages:

العلاقة بين فيروس كوكساكي ب وزيادة خطر المناعة الذاتية لدى مرضى داء السكري النوع الاول == Association between Coxsackie B viruses and islet autoimmunity in patients with type 1 diabetes mellitus (T1DM

Author name: شمس صلاح الدين مهلهل

Supervisor name: وفاء سعدون شاني | حيدر عبد الحسين مكلف

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University Of Basrah - College Of Science - Department Of Biology

Language: English

University location: Basrah

First pages:

تثبيط تعبير جينات fimH ,fimD ,fimC في بكتريا E. coli الممرضة للمجاري البولية باستعمال جسيمات ثنائي اوكسيد التيتانيوم النانوية المصنعة حيويا من المعززات الحيوية البكتيرية == Inhibition the Expression of fimC, fimD, fimH Genes in Uropathogenic E. coli Using TiO2 Nanoparticles Biosynthesized by Probiotics Bacteria

Author name: سوزان عبد الرحيم حسن

Supervisor name: سوسن ساجد الجبوري | جيهان عبد الستار سلمان

General topic: Biology

Specific topic: Microbiology

Degree: Doctorate

University: Mustansiriyah University - College Of Science

Language: English

University location: Baghdad

First pages:

Abstract: Summary ►Urinary tract infections (UTIs) are the commonest human bacterial infections and are responsible for substantial morbidity and mortality, resulting in increased healthcare costs. Specialized Escherichia coli strains referred to as Uropathogenic E. coli (UPEC) causes most UTIs. ►Total 120 isolates primarily identified as E.coli; were collected from (December 2018 to February 2019). Out of total collected isolates, 94/120 were included in this study and the contaminated bacteria or weak growth were excluded. The isolated pure colonies were re-identified using some standard morphological tests followed by genetically identification using housekeeping gene (HKG) 16S rDNA. The results confirm that all the isolates were definite as E. coli. ►The UPEC isolates showed their ability of adherence to uroepithelial cells. The aggregation ability of bacterial cells were appeared as dotes on the surface of epithelial cells. ►The antimicrobial susceptibility test (AST) was performed for 50 isolates under study. The highest resistance rate was concerning to Aztreonam; Cefazolin; Ceftazidime and Ceftriaxone (84%), Cefepime (82%), while the minimum resistant was for Imipenem; Ertapenem; Meropenem; Amikacin when it was (2%) beside that none of the isolates was able to resist Tigecycline. According to AST for fifty UPEC isolates; (88%) were multidrug resistance (MDR), while (2%) was extensively drug resistance (XDR) and (10%) were sensitive to all antibiotics thus they were non-drug resistant (NDR). ►All isolates were subjected to detect type1 fimbriae genes (fimA operon) by polymerase chain reaction using specific primers (designed in this study) followed by sequencing the amplified fragment and analyzed using Geneious prime software. The results showed that all the isolates (100%) were positive for fimA, fimI, fimC, fimD, fimG, fimH, while (96%) for fimB, fimF and (82%) for fimE. ►Raw milk samples were collected for probiotics bacteria isolation. All isolates were subjected to the cultural, microscopically, biochemical tests and Vitek2 system. Results indicated that six isolates of Lactococcus spp. Identified as: Lactococcus lactis ssp. lactis, in addition, 14 isolates of Leuconostoc spp. distributed among species as (8) isolates belonged to Leuconostoc mesenteroides ssp. cremoris (3) isolates belonged to Leuconostoc mesenteroides ssp. mesenteroides, (2) isolates identified as Leuconostoc pseudomesenteroides and only one as Leuconostoc citreum. ►All isolated probiotics bacteria were tested for Titanium dioxide (TiO2) nanoparticles production. The results showed that they had the ability to biosynthesize TiO2 NPs, biosynthesized TiO2 NPs were characterized using UVVis Spectroscopy; Atomic Force Microscope (AFM); X-ray Diffraction (XRD); Field Emission Scanning Electron Microscopy (FE-SEM) Energy dispersive Xray spectroscopy (EDX); and Fourier Transform-Infrared Spectroscopy (FTIR). The detected absorption spectrum with a wide-ranging absorption band in UV range and its cut off wavelength was (344-348) nm for all 20 probiotic isolates. ►The smoothness and roughness of all samples of biosynthesized TiO2 NPs was detected by AFM with smoothest sample for L. mesenteroides ssp. cremoris (Leu.4) as 1.137nm. XRD results indicated the formation of nano anatase crystals. ►The Scanning electron microscope images of biosynthesized TiO2 NPs biosynthesized by probiotics bacteria; that were consists of a uniform distribution of spherical shaped nanostructure crystals with a smallest size of (37.48) nm for L. citreum (Leu. 13). ►The EDX analysis of TiO2 NPs existing in medium which confirm that all the samples contained the elements (Ti, O), which again established that the biosynthesized NPs were pure TiO2. ►The determination of functional group of biosynthesized TiO2 NPs studied using FTIR. The main IR absorption peaks of TiO2 biosynthesized by probiotics bacteria were in range at (408- 445) cm-1; these peaks belonged to anatase Titania. ►The biosynthesized TiO2 NPs at concentrations (1000- 0.001) mg/mL were tested for its antibacterial activity against UPEC isolates, thus there was neither bactericidal effect against pathogenic bacteria nor been as minimum inhibitory concentration (MIC). ►There was no effect of temperature, pH and photocatalytic activation on antibacterial activity of biosynthesized TiO2 NPs against clinical UPEC isolates. ►Regardless of no growth inhibition activity showed on culture media after subjecting bacterial UPEC isolates to TiO2 NPs. There was a noticeable inhibitory effect of TiO2 NPs on the attachment ability of bacterial cells without killing them. The highest inhibition was recorded at the higher concentration of biosynthesized TiO2 NPs (1000 g/mL) with inhibition reached 92% and 90.3%, while the lowest inhibition were 52% and 54.8% at 250g/mL against E.coli74 and E.coli23, respectively. ►The effect of biosynthesized TiO2 NPs by probiotics bacteria and commercial TiO2 NPs was studied on the expression of fim genes. There was a subsequent change in the gene expression in term of folding and CT values when the expression of fimH, C, D genes start to decrease by increasing concentration of TiO2 NPs as compared with control and the maximum inhibitions was noticed by using 1000 g/mL concentration of the NPs specially against fimD.

Summary:

References:

Phenotyping and Molecular Characterization of Proteus vulgaris Isolated from Patients with Urinary Tract Infections

Author name: Ghaida’a Jihadi Mohammed

Supervisor name: Abdallah K. Hindi | Wang Yanchang

General topic: Biology

Specific topic: Microbiology

Degree: Doctorate

University: University of Babylon - College Of Science - Department Of Biology

Language: English

University location: Babylon

First pages:

دراسة مقاومة بعض عزلات السالمونيلا للمضادات الحيوية وحساسيتها لبعض المستخلصات النباتية في مدينة السماوة == Resistance Study of Some Salmonella Isolates to Antibiotics and its Sensitivity to Some Plant Extract in Samawa City

Author name: علاء فاروق رسول

Supervisor name: علي عنوك نجم

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: Al-Muthanna University - College Of Science - Department Of Biology

Language: English

University location: Muthanna

First pages:

الكشف المناعي والجزيئي لبكتريا الدوران والسايتوميكالو فيروس عند النساء المجهضات في محافظة المثنى == Immunological and Molecular Detection of HCMV and Listeria monocytogenes of Miscarriaged Women in Al Muthanna Governorate

Author name: ساره زغير حسين

Supervisor name: طارق جعفر فعل جندي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: Al-Muthanna University - College Of Science

Language: English

University location: Muthanna

First pages:

الاستجابة المناعية المتاصلة المرتبطة بالتهابات المجاري البولية لدى مرضى السكري في محافظة المثنى == Innate Immune Response Associated with Urinary Tract Infection Diabetes in Al-Muthanna Province

Author name: هدى طاهر علي

Supervisor name: وئام سعد الحمداني

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: Al-Muthanna University - College Of Science - Department Of Biology

Language: English

University location: Muthanna

First pages:

الكشف عن عوامل ضراوة بكتيريا الزوائف الزنجارية المعزولة من اصابات الجروح ودراسة تاثير جسيمات الفضة النانوية عليها == Detection of virulence factors of Pseudomonas aeruginosa isolated from wound infections and study of the effect of silver nanoparticles on them

Author name: قحطان عدنان قادر محمد ال حسون البياتي

Supervisor name: بشار صادق نومي | ريام فارس صالح

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: Tikrit University - College Of Science - Department Of Biology

Language: Arabic

University location: Salahaddin

التوصيف المظهري والجزيئي للفطر الاسود المصاحب لكوفيد 19 وتقييم بعض الطرق للسيطرة عليه مختبريا == Morphological and Molecular Characterization of Black Fungus (Mucormycosis) Associated with Covid-19 and Evaluate Some Methods for Controlling it in vitro

Author name: ختام ابراهيم محمد

Supervisor name: نيران عبيد جاسم

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Al-Qadisiyah - College Of Science - Department Of Biology

Language: English

University location: Qadisiyah

First pages:

التحري الجزيئي عن بعض جينات الضراوة لبعض الفطريات المحمولة على الملابس المستعملة == Molecular detection of some virulence factors of some fungi associated with second hand clothes

Author name: رقية حسين عبد الزبيدي

Supervisor name: ماجد كاظم الشبلي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Al-Qadisiyah - Faculty Of Education - Department Of Biology

Language: Arabic

University location: Qadisiyah

First pages:

تاثير بعض مضادات الميكروبات على نمو وتكوين الاغشية الحيوية من Enterococcus faecalis وEscherichia coli المعزولة من النساء المصابات بالتهاب المهبل == The Effect of Some Antimicrobial Agents on Growth and Biofilm Formation of Enterococcus faecalis and Escherichia coli Isolated from Women with Vaginitis

Author name: اسراء سامي عثمان

Supervisor name: ايمان عبعوب مخيفي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University Of Basrah - College Of Science - Department Of Biology

Language: English

University location: Basrah

First pages:

دراسة انتشار وتشخيص وضراوة الاصابات الفطرية المكتسبة في مستشفيات البصرة == Prevalence, identification, and virulence study for nosocomial fungal infections in Basrah hospitals

Author name: فؤاد كاظم عبيد

Supervisor name: انعام محمود نجم الربيعي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University Of Basrah - College Of Science - Department Of Biology

Language: English

University location: Basrah

First pages:

دراسة بعض عوامل الضراوة للبكتريا السائدة والمرافقة لمرض التهاب الاذن الوسطى == Study of Some Virulence Factors for Most Prevalent Bacteria associated with Otitis Media

Author name: نور حافظ احمد

Supervisor name: زينب راضي عبد الحسين

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University Of Basrah - College Of Science - Department Of Biology

Language: English

University location: Basrah

First pages:

دراسة جرثومية وجزيئية لتشخيص البكتريا المسببة لتسوس الاسنان في مدينة العمارة == Bacteriological and Molecular Study to Identify the Bacteria that Cause Dental Caries in Al-Amirah City

Author name: محمد عباس عبد علي سبهان الساعدي

Supervisor name: علي عبود شريف

General topic: Biology

Specific topic: Microbiology

Degree: Doctorate

University: University Of Basrah - College Of Education For Pure Sciences

Language: English

University location: Basrah

First pages:

عزل وتشخيص والتحري بعض عوامل الضراوة لبكتريا الحلزونية البوابية == Isolation, Identification and Screening of Some Virulence Factors of Helicobacter pylori

Author name: زينب ناصر محمد رضا مهدي

Supervisor name: Lina Abbas Naser

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University Of Basrah - College Of Science - Department Of Biology

Language: English

University location: Basrah

First pages:

التشخيص الجزيئي وانتشار فيروس (TTV) Torque Teno وفيروس (SENV) SEN في مرضى التهاب الكبد والمتبرعين بالدم في البصرة / العراق == Molecular detection and prevalence of Torque Teno Virus (TTV) and SEN Virus (SENV) in Hepatitis Patients and Blood Donors in Basrah/Iraq

Author name: خالد ناصر ديوان

Supervisor name: حيدر عبد الحسين الحمودي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University Of Basrah - College Of Science - Department Of Biology

Language: English

University location: Basrah

First pages:

دراسة مظهرية وجينية للبايوفيلم الذي تكونه المكورات العنقودية الذهبية المقاومة المعزولة من الاصحاء ومرضى التهاب اللوزتين == Phenotypic and Genotypic investigation of Biofilm forming methicillin resistance of Staphylococcus aureus Isolated from Healthy and Tonsillitis Patients

Author name: ميسون خليل ابراهيم الحيدري

Supervisor name: سعد شاكر مهدي الامارة | مرتقب يونس الحجاج

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University Of Basrah - College Of Science - Department Of Biology

Language: English

University location: Basrah

First pages:

تحديد بعض عوامل الضراوة لبكتريا Pseudomonas aeruginosa المعزولة من مرضى الحروق والبيئة المحيطة بهم ودراسة الفعالية البايولوجية لبعض المستخلصات الطبيعية عليها == Detection of some virulence factor of Pseudomonas aeruginosa isolated from Burn' patients and their surrounding environment and the biological activity of some extracts on it

Author name: رغد جميل مهدي

Supervisor name: ياسين يعوب يوسف اللعيبي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University Of Basrah - College Of Science - Department Of Biology

Language: English

University location: Basrah

First pages:

دراسة الاخماج البكتيرية ودور بعض المؤشرات الحيوية في التحري الاولي عند مرضى سرطان الاطفال تحت العلاج الكيميائي في مدينة البصرة / العراق == Study of Bacterial Infections and the Role of some Host Biomarkers in Early Detection among Febrile Pediatric Cancer Patients under Chemotherapy in Basrah Province- Iraq

Author name: دلال عباس هادي

Supervisor name: حسام محمود صالح | اماني عبد الرضا عوض العبد الله

General topic: Biology

Specific topic: Microbiology

Degree: Doctorate

University: University Of Basrah - College Of Science - Department Of Biology

Language: English

University location: Basrah

First pages:

عزل وتشخيص الدنا البكتيري المندمج في دنا مرضى السرطان

Author name: لمياء عبد الحسين عبد الرضا

Supervisor name: مناف جودة عبد العباس

General topic: Biology

Specific topic: Microbiology

Degree: Doctorate

University: University Of Basrah - College Of Science

Language: English

University location: Basrah

First pages:

دراسة وبائية مصلية لخمج فايروس التهاب الكبد نمط ب بين مرضى التهاب الكبد الفايروسي الحاد والكادر الطبي في البصرة == SEROEPIDEMIOLOGY OF HEPATITIS B VIRUS (HBV) INFECTION AMONG PATIENTS WITH ACUTE VIRAL HEPATITIS AND MEDICAL STAFF IN BASRAH

Author name: غيداء كاظم باقر

Supervisor name: SABAH M. SHAMSHOOM

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University Of Basrah - College Of Science - Department Of Biology

Language: English

University location: Basrah

First pages:

المفطورات التناسلية في النساء المراجعات لمستشفى البصرة العام وتقييم علاقتها بحالات مرضية منتخبة == Genital Mycoplasmas among women attending Basrah General Hospital with an evaluation of their role in selected clinical cases

Author name: ريهام محمد مصطفى الموسوي

Supervisor name: amin a. al-sulami

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University Of Basrah - Faculty Of Education

Language: English

University location: Basrah

First pages:

استخلاص وتنقية بروتين الكلايدين من صنف حنطة محلي كمستضد واستخدامه في اختبار المعايرة الامتصاصية المناعية المرتبطة بالانزيم (ELISA) لتشخيص مرض الحساسية المعوية للحنطة == Extraction and purification of gliadin protein from local variety wheat as a specific antigen and using it in the Enzyme-Linked Immuno^ Sorbent Assay (ELISA) as serological test for diagnosis Celiac disease

Author name: ايناس صباح محمد شريف

Supervisor name: عواطف حميد

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University Of Basrah - College Of Science - Department Of Biology

Language: English

University location: Basrah

First pages:

استخلاص وتقنية m.protein من عزلة محلية لجرثومة streptococcus pyogenes كمستضد نوعي واستخدامه في اختبار المعايرة الامتصاصية المناعية المرتبطة بالانزيم enzyme linked immunosorbent assay لتشخيص الحمى الرثوية == Extraction and Purification of the role of streptococcal M-protein Antigen and Using it in the Enzyme - Linked Immunosorbent Assay in The Serological Diagnosis of Rheumatic Fever

Author name: زينب صبيح وريوش الحواس

Supervisor name: عواطف حميد

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University Of Basrah - College Of Science - Department Of Biology

Language: English

University location: Basrah

First pages: