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دراسة التعبير المناعي ل C4d وفيروس BK ووجود الاجسام المضادة الخاصة بفيروس BK في الخزعات الكلوية ومصل الدم من مستلمي زرع الكلى العراقيين == A study of the immunological expression of C4d, BK virus and the presence of anti-BK virus Antibodies in biopsies and serum of Iraqi renal transplant recipients

Author name: طه ياسين مهدي
Supervisor name: رفيف صبيح جمال | ثائر جواد كاظم
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:

التحديد الجزيئي لانزيمات الميتالوبيتا لاكتميز المهمة طبيا والمنتجة من قبل بكتريا الزوائف الزنجارية والكلبسيلا الرئوية متعددة المقاومة للادوية == Molecular detection of medically important Metallo- ?-lactamases produced by multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumonia

Author name: شهد علاء الدين حسين الطائي
Supervisor name: مشتاق طالب صالح العكيلي | عامر رشيد النجار
General topic: Medicine
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University
Language: English
University location: Baghdad
First pages:

عزل وتجنيس فايروس مرض نيوكاسل محليا من قطعان الدواجن المصابة طبيعيا في شمال العراق == ISOLATION AND GENOTYPING OF LOCAL NEWCASTLE DISEASE VIRUS ISOLATED FROM NATURALLY INFECTED CHICKENS IN NORTHERN IRA

Author name: احمد ابراهيم احمد
Supervisor name: شوني ميخائيل اوديشو | رباح نجاح جبار
General topic: Veterinary Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University of Baghdad - College Of Veterinary Medicine
Language: English
University location: Baghdad
First pages:
Abstract: Newcastle disease (ND) has been concerned as one of the most important devastating diseases of poultry because of its wide range host of birds and worldwide distribution with severe economic losses in domestic poultry, due to the severe nature of the disease, ND is included as a list A disease.
Iraq has been endemic for Newcastle disease virus (NDV) with natural infection causing significant losses in the poultry industry since 1968 by the NDV/AG68 strain (AF001108) from infected chickens at Abu Graib in Iraq.
Currently, NDV genotype VII are most frequently associated with outbreaks of ND in the Middle East and Asia which have showed higher mortality in vaccinated poultry. The main objective of this study was to determine the biological and molecular characterization of the pathogenicity and genotyping followed by phylogenetic analysis. To evaluating the degree of genetic diversity of local isolated NDV strains and estimating the relationships to that NDV presence in the neighboring region, as well as preparation of inactivated vaccine against NDV from local strains was determine.
At the beginning, this study was carried out to isolate Newcastle disease virus (NDV) in naturally infected chickens of poultry farms in the north of Iraq. The study initiated diagnosis of 46 farms out of 96 broiler farms exhibited Newcastle disease (ND) (26 in Erbil, 14 in Sulaymanhia and 6 in Duhok) based on clinical signs corresponding with (8-15%) mortality and gross lesions of scarified chickens. The rapid test for detection NDV antigen from infected chickens for tracheal swab indicated that 32 farm samples were positive out of 46 initiative diagnosed farms.
Based on intiative diagnosis the identification of local isolates of NDV samples was pooled lung with trachea, spleen, cecal tonsil and intestine, were pooled in nine samples, then tissue homogenates were made from all samples in sterile Phosphate Buffered Saline (PBS) containing antibiotics, after centrifugation of tissue homogenates the supernatant fluids were assessed to real time reverse transcriptase- Polymerase Chain Reaction (RRT-PCR) assay. The results indicated that six
homogenate samples were positive for NDV and negative for Avian Influenza Virus.
After that, the remain supernatant fluids were used for virus isolation in embryonated chicken eggs then harvested allantoic fluids were used for employed for the hemagglutination activity. Six samples were showed positive results. Furthermore, virus identification was also performed by using conventional diagnostic methods such as Hemagglutination(HA) of red blood cells followed by Hemagglutination Inhibition (HI) test applying the prepared hyper immune serum(HIS) against NDV antigens to identify the NDV. The serological results demonstrated that six samples of NDV were positive, as well as the (rt-PCR) assay was performed for allantoic fluid to insure the presence of NDV using specific primers and probes to amplify specific region in the M gene of the viruses. The results revealed that six isolates were positive for NDV.
In addition, the pathogenicity of the six isolates was performed according to standard methods including Mean death times (MDT), Intra-cerebral pathogenicity index (ICPI) and Intra-venous pathogenicity index(IVPI), which revealed that six isolates were compatible to mesogenic (1/6) and velogenic (5/6) types.
At the second step, the molecular characterization of six isolates were investigated in Borehingher laboratory (Germany). The RT-PCR successfully amplified a 436 bp DNA fragment covering a part of the matrix (M) gene, fusion (F) gene (lentogenic) and fusion (F) gene (subgenotype VIIi) from all six isolates (NDV). The result was indicated that 5/6 were positive for velogenic NDV strain of genotype VIIi and 1/6 were negative. The nucleotide sequences of F gene were also determined, where the portion of the F2 gene of five isolates compared with that of other NDV strains retrieved from GenBank. Phylogenetic analysis showed that the isolates NDV belong to genotypes VIIi.
However, in local laboratory the result of FAST one-step quantitative Polymerase Chain Reaction (qPCR) successfully amplified a 525 bp fragment covering a part of the fusion (F1) gene and 515 bp fragment covering a part of the fusion (F2) gene from all six isolates. The nucleotide sequences of the PCR product of E1, S1 and S2
were found in F1 gene, while E1, E2, S1, S2, and D2 found in F2 gene out of six NDV isolates. Those results were compared with other NDV strains which retrieved from GenBank. Phylogenetic analysis showed that the Iraqi isolates are belonging to genotypes VII.
Finally, Inactivated vaccine was prepared locally using Velogenic NDV genotype VIIi isolate as a seed virus ELD50/0.1ml109 then compared with a commercial inactivated vaccine in an experimental study. This study was conducted by purchasing 120 broiler chicks and divided into three equal groups. Group 1 as unvaccinated control group , G2 for commercial vaccine and G3 for local vaccine), G2 and G3 were injected s/c with inactivated vaccine at 3 days old followed by a booster dose with LaSota live vaccine eye drop. Indirect ELISA technique was used to measure of antibody titer at ages 7, 17 and 27 days old (pre-challenge), the result showed at 7 days-old were (4251±1078) for all groups, and at 17 days-old were (906±33, 844±153 and 1145±219) for G1, G2 and G3 respectively. There were significant differences (p
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تحضير بوليمر قابل لمتاكل الحيوي من حامض اللاكتيك باستخدام البكتريا المنتجة لحامض اللاكتيك == Preparation of Biodegradable Polymer of Lactic Acid using Lactic Acid Producing Bacteria

Author name: وصال عودة حسن الحلفي
Supervisor name: ميثم ايوب الحمداني | امال كاظم الاسدي
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: English
University location: Basrah
First pages:

التشخيص المصلي والكشف الجزيئي لبكتريا المكورات العنقودية الذهبية المقاومة للمثيسيلين المعزولة من مرضى الحروق في مستشفى الحسين التعليمي في محافظة ذي قار == Serological Diagnosis and Molecular Detection of Methicillin Resistant Staphylococcus aureus Isolated from Burn Patients in Al-Hussein Teaching Hospital in Thi-Qar Province

Author name: احمد نهاد عبد
Supervisor name: سعد سلمان هميم
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Thi-Qar - College Of Science - Department Of Biology
Language: English
University location: Dhi Qar
First pages:

الاشكال السريرية والدلائل الحيوية للروماتيز مجهول السبب في اليافعين مقارنة بالروماتيز الرثوي في البالغين في عينة من مرضى عراقيين

Author name: حنان ياسين محسن
Supervisor name: منى تركي الموسوي | علي حسين ادحية
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

تاثير Lactobacillus reuteri وروترسين على Salmonella enterica و Shigella flexneri في الكائن الحي وفي الزجاج == Effect of Lactobacillus reuteri and its reutericin on Salmonella enterica and Shigella flexneri in vivo and in vitro

Author name: سارة عبد الكريم جابر
Supervisor name: لقاء حميد مهدي الموسوي
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

الكشف المظهري والوراثي لانزيمات البيتالاكتاميز نوع AmpC في بكتريا Enterobacter spp المعزولة سريريا == Phenotypic and genotypic detection of AmpC ? - lactamase among clinical isolates of Enterobacter spp.

Author name: لمياء محمد جاسم الحسيني
Supervisor name: سعد لعيبي حامد
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

انتشار الفايروس المضخم للخلايا في النساء العراقيات غير المتزوجات المصابات بداء السكري النوع الثاني == Prevalence of Human cytomegalovirus infection in non married Diabetic type Iraqi women

Author name: همسة اسعد فرج
Supervisor name: ايمان ناطق ناجي
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

علاقة بعض عوامل الضراوة و العوامل البيئية مع تكوين الغشاء الحيوي لبعض البكتريا الممرضة المعزولة من اصابات الاذن == Correlation of some virulence and environmental factors with biofilm formation in some pathogenic bacteria isolated from ear infections

Author name: ﻫﺒﺔ ﻣﺤﻤﺪ ﻋﺒﺪ الحسن
Supervisor name: ﻧﻬﺎﯾﺔ ﺣﻜﻤﺖ زكي
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

تاثير متعدد السكرايد الخارجي المنقى من العزلة المحلية Lactobacillus reuteri على بعض العزلات المقاومة للمضادات الحيوية Klebsiella pneumoniae == Effect of exopolysaccharide purified from locally isolates Lactobacillus reuteri on some isolates multidrug resistant Klebsiella pneumoniae

Author name: هالة اياد محمد علي
Supervisor name: لقاء حميد مهدي
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

دراسة العلاقة بين الجينات المشفرة لبعض عوامل الضراوة وبعض المؤشرات المناعية لبكتريا Klebsiella pneumoniae و Escherishia coli المعزولة من اصابات الجهاز البولي == The Correlation between Some Virulent Factors Encoding Genes and Various Immune Parameters of Klebsiella pneumoniae and Escherishia coli Isolated from Urinary Tract Infections

Author name: نور نعيم خوين الربيعي
Supervisor name: علي حسين علوان العامري
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

التحري عن الجينات المشفرة لانزيمات الكاربابينميميز في بعض البكتريا السالبة لصبغة غرام

Author name: نور عادل حسون
Supervisor name: سعد لعيبي حامد
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

تحديد اليل HL A وتقييم مستوى الحركيات الخلوية IL - 12 و IL - 17A لدى مرضى حساسية الحنطة == Detection of HLA - DQB1 and Assessment of Interleukins 12 and 17A in patients with Celiac Disease

Author name: نور صباح حافظ
Supervisor name: اخلاص نوري علي
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

دور انزيم الكاسبيز - 1 في الاستجابة الالتهابية ضد بكتريا Pseudomonas aeruginosa من مصادر سريرية مختلفة == The role of Caspase - 1 in inflammatory response against P.aeruginosa infection from different clinical sources

Author name: نور ابراهيم كاظم
Supervisor name: علي حسين علوان
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

دور بعض المضادات الحياتية والكلايسين في انتاج اليوريز من بكتريا Helicobacter pylori المعزولة من المرضى المصابين بالقرح المعدية == Role of some antibiotics and glycine on urease production in Helicobacter pylori isolated from peptic ulcer patients

Author name: نادية عامر صادق النعيمي
Supervisor name: سوسن حسن عثمان | حيدر صباح الكواز
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

التاثير السمي الخلوي لانزيم البروتييز المعدني المنقى والمنتج من Aeromonas من hydrophila على بعض خطوط الخلايا السرطانية == Cytotoxicity role of purified Metalloprotease produced by Aeromonas hydrophila on some tumor cell lines

Author name: مهند معين عبد الله
Supervisor name: تحرير هادي صالح
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

العلاقة بين جينات تكوين الغشاء الحيوي و انتاج البايوسيانين في بكتريا Pseudomonas aeruginosa المعزولة من مصادر سريرية == The relationship between biofilm formation and pyocyanin producing genes in Pseudomonas aeruginosa isolated from clinical sources

Author name: مها مخلف كاظم الشمري
Supervisor name: محمد فـــرج المـــرجاني
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

تاثيــر البوليمر الحـــيوي الدكستران المــنتج مــن بكــــتريا Leuconostoc mesenteroides ضد البكتريا المرضية المعزولة من ادوات القسطرة البولية == Effect of Biopolymer dextran produced from Leuconostoc mesenteroides against pathogenic bacteria isolated from catheters

Author name: مصطفى زين الدين سالم عبد
Supervisor name: جيهان عبد الستار سلمان
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

طفرات مجاور اللب وحفاز اساس اللب لفايروس التهاب الكبد ب وعلاقته بالاصابات المقاومين للعلاج في بعض المرضى العراقيين == Precore and Basal Core Promoter mutations of chronic Hepatitis B virus in relation to drug resistance chronic infections in some Iraqi patients

Author name: مصطفى جواد كاظم
Supervisor name: كاظم هاشم ياسين الاعرجي | عباس محمود احمد الجاف
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

التاثير السمي لانزيم الكايتينيز المنقى من بكتريا Aeromonas hydrophila على بعض خطوط الخلايا السرطانية == Cytotoxicity effect of purified Chitinase from Aeromonas hydrophila on some cancer cell lines

Author name: محمود محمد نعمه
Supervisor name: صبا طالب هاشم
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

دور 155.146a والحركيات الخلوية الالتهابية والمضادة للاتهاب على استقطاب الخلايا البلعمية في عينة من مرضى القلب

Author name: محمود محمد مصطفى
Supervisor name: اقبال خضر الجوفي
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

عزل وتشخيص مجموعة Bacillus subtilis من الترب العراقية وتقييم خصائها المضادة للمايكروبات == Isolation and Identification of Bacillus subtilis group from Iraqi soil and Evaluation of their Antimicrobial properties

Author name: محمــد صكبان باقــر
Supervisor name: امل حسين موسى
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

استخلاص وتنقية المضاد الحيوي Pyoluteorin المنتج من قبل بكتريا Pseudomonas aeruginosa المعزولة من جذور النباتات وتاثيرة على المايكروبات المسببة لالتهابات المجاري البولية == Extraction and purification of pyoluteorin antibiotic produced by rhizospheric Pseudomonas aeruginosa and their effect on urinary tract infection pathogen

Author name: ماجد رزيج زعيط
Supervisor name: امل حسين موسى
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

تاثير انزيم الهايلورونديز الخام المنتج من بكتريا المكورات العنقودية الذهبية تجاه عزلات اصابات الجهاز التنفسي == Effect of crud hyaluronidase enzyme which produced by Staphylococcus aureus against respiratory infection isolates

Author name: لمى سليم حاجم
Supervisor name: خولة جبر خلف
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:
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