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التباين الوراثي لجين E - selectin المصاحب للاصابه بمرض السكري من النوع الثاني في عينه من الذكور العراقيين العرب

Author name: امال يوسف جبار احمد
Supervisor name: اسماء محمد صالح المهيدي
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: Diabetes - Type II (Diabetes Mellitus type 2) is a group of metabolic diseases resulting from high blood sugar because of a defect in insulin secretion, insulin action, or both, and tissue resistance to insulin. This study was conducted to evaluate the serum level of factor E - selectin in patients with diabetes and its relationship to the polymorphisms genetic Jene E - selectin A561C at the site and assess the relationship between classical factor E - selectin factors.The study included 100 male appointed to the Iraqis. The first group (80) patients with diabetes type II and second group (20) of healthy as control samples. The study was conducted at Yarmouk Teaching Hospital / Central Laboratories and Hospital of city Alkazemian, and health center in the Saidiya. For the period from November 2015 until February 2016.We have been identified patients with diabetes type II and control in terms of age, gender, family history of the disease and the duration of diseases and weight (kg) and height (cm) and waist central obesity (cm) And we have been calculate the body mass index calculation. The serum for both groups ,we calculate the level of sugar in the blood and hemoglobin A1c and cholesterol and triglycerides and high - density fat and fat and a low - density and fat and very low - lying density index of atherogenic index.Blood sugar and hemoglobin A1c showed significant differences between patients and control while the cholesterol and fat of all kinds showed no significant differences, except for fat, high - density non - significant differences compared to control patients.It was calculate by the concentration of the adhesion molecule E - selectin in the patients' blood serum (66) and compared to control a person (20) individual using the ELISA device and results showed significant differences in patients than controls.When divided the patients into three groups totals for minor according to the level of the hemoglobin A1c in the blood serum. The first group was less than 6.5, and the second group was between (6.5 to 9.3), the third set was more than 9.3. Control group was divided into two subsections, according to the level of hemoglobin A1c , the first group was less than 6.5, and the second group was between (6.5 to 9.3).SummaryAppeared to increase most of the variables in the third set when the level hemoglobin A1c of more than 9.3.When you link current study results have shown that the sugar in the blood record significant correlation with the body mass index (0.20). The hemoglobin A1c and creatine and cholesterol and low - density lipids significant correlation with central obesity respectively coefficient (0.20) (0.22) (0.23) (0.21) and E - selectin factor (0.21).The molecular study included 74 individual patients and (20) of individual control. It included PCR - RFLP technique. Electric deportation results for Jene E - selectin at the site A561C showed the presence of alleles are A, C and the presence of three genotypes are (AA, AC, CC). Allele A is a higher proportion of allele C in patients and control, and that repeating models genetic (AA, AC , CC) with no significant differences in the patient group than controls. Showed allele A difference no significant in control compared to patients using the test Fisher and relying on the Confidence Intervals (CI) and showed a preventive fraction (PF) of the disease, and that the allele C showed a no significant difference in patients than controls using test Fisher depending on the Confidence Intervals (CI) and showed that the allele C was Etiological fraction (EF). Repeat genotype AA difference is no significant in control compared to patients style of preventive fraction (PF) of disease, showed a repeat genotype AC difference is no significant in patients than controls and appeared style of Etiological fraction (EF) of disease, showed a repeat genotype CC no significant difference in patients than controls and Etiological fraction (EF) of disease. and can be used as indicators of readiness of members of Iraqi society of the male to the disease ..From the study of the genotype to Jene E - selectin at the site A561C a number of the details in the totals for the study, found that there were significant differences between patients and control genotype structures the three (AA, AC, CC) with sugar and E - selectin and hemoglobin A1c , and that there is differences were not significant with the body mass index.

دراسة علاقة مجاميع الدم ABO مع البين ابيضاضي 18 المرتبط بالبروتين الفا والبين ابيضاضي 18 مستقبل البيتا في النساء العراقيات العربيات المصابات بالسكري من النوع الثاني == Studying the Association of ABO Blood Groups with IL - 18BPa & IL - 18R beta in the Iraqi Arab Females with Diabetes Mellitus Type 2

Author name: زينب فاضل عبد الغفور
Supervisor name: اسماء محمد صالح المهيدي
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: داء السكري كمرض التهابي يرتبط مع العديد من العوامل الالتهابية لذلك تهدف الدراسة الحالية لحساب تركيز العامل البين ابيضاضي 18 المرتبط بالبروتين الفا والعامل البين ابيضاضي 18 مستقبل البيتا مع عوامل الخطورة التقليدية والتي تشمل المقاييس الفيزيائية مثل متوسط محيط الخصر ومؤشر كتله الجسم وعوامل البيو كيميائية مثل الانسولين , مقاومة الانسولين , مستوى السكر في الدم في حاله الصيام ,مستوى الهيموغلوبين السكري وملف الدهون وفي الوقت نفسه دراسة ارتباطهم مع نظام الدم ABO في النساء العراقيات العربيات المصابات بالسكري من النوع الثاني ومن اجل معرفة تاثير ذلك مع عوامل اخرى التي تعتبر كمؤشر او مطور لمرض السكري من النوع الثاني . وشملت الدراسة الحالية 340 مريضا من النساء العراقيات العربيات المشخصات حديثا بالسكري من النوع الثاني 200 من النساء الاصحاء اختبرت للتكرار الاليلي والمظهري ,ومن هؤلاء 60 مريضة اختبرت للدراسة المناعية والكيميائية مقارنة مع 28 من النساء الاصحاء .الاختبارات المناعية بواسطة استخدام تقنية الانزيم المرتبط المناعي, اما المقاييس الكيميائية باستخدام الاساليب الانزيمية واللونية, وتم تقسيم المرضى والسيطرة حسب مجاميع الدم الى اربعة مجاميع ثانوية (A , B ,AB ,O) وفقا لنوع فصائل الدم التي كشف عنها بواسطة الاختبارات المصلية القياسية .اظهرت البيانات الحالية : 1 - زيادة معنوية في المصل العامل البين ابيضاضي 18 المرتبط بالبروتين الفا في المرضى الحاليين ,في حين لم تظهر اختلافات معنوية في مستوى مصل العامل البين ابيضاضي 18 مستقبل البيتا .2 - زيادة معنوية في متوسط العامل البين ابيضاضي 18 المرتبط بالبروتين الفا مع زيادة العمر في كل من المرضى ومجموعه السيطرة, في حين العامل البين ابيضاضي 18 مستقبل البيتزااد في المجموعة الاقل من 40 سنة وانخفض مع زيادة العمر في كل من المرضى ومجموعه السيطرة .بالاظافة الى زيادة كل عوامل الخطورةالاخرى.3 - تركيز كلا العاملين العامل البين ابيضاضي 18 المرتبط بالبروتين الفا والعامل البين ابيضاضي 18 مستقبل البيتا يزداد بشكل غير معنوي تدريجيا في كل المرضى مع زيادة مدة المرض ونفس النتيجة لمقاومة الانسولين.4 - اظهرت نسبة الهيموغلوبين السكري اقل من 7.3 % زيادة كبيرة في متوسط العامل البين ابيضاضي 18 المرتبط بالبروتين الفا , العامل البين ابيضاضي 18 مستقبل البيتا وتنخفض تدريجيا مع زيادة نسبة الهيموغلوبين السكري .5 - الافراد الذين كانوا يحملون فصيلة الدم B اظهروا اعلى معدل لمتوسط العامل البين ابيضاضي 18 المرتبط بالبروتين الفا , الانسولين ومقاومة الانسولين .6 - المرضى الذين كانوا يحملون فصيلة الدم B والاصحاء الذين يحملون فصيلة الدم A اظهروا اعلى معدل لمتوسط العامل البين ابيضاضي 18 مستقبل البيتا .7 - يعتبر العامل البين ابيضاضي 18 المرتبط بالبروتين الفا والعامل البين ابيضاضي 18 مستقبل البيتا كعوامل خطورة مستقلة في النساء العراقيات العربيات المصابات بالسكري من النوع الثاني والتي تدعمها غياب العلاقة المعنوية بين العامل البين ابيضاضي 18 المرتبط بالبروتين الفا والعامل البين ابيضاضي 18 مستقبل البيتا وجميع عوامل الخطورة التقليدية الاخرى .8 - هنالك علاقة ايجابية بين مستويات العامل البين ابيضاضي 18 المرتبط بالبروتين الفا وخطورة السكري من النوع الثاني والذي قد يعتبر كمؤشر لحدوث المرض , في حين ان مستويات العامل البين ابيضاضي 18 مستقبل البيتا قد يكون مؤشر لتطور المرض .مزيد من الدراسات اللازمة لفهم العلاقة بين مجاميع الدم , عوامل الالتهابية ومخاطر السكري من النوع الثاني . | DM is considered as inflammatory disease associated with many inflammatory factors. As such the present study aims to investigate the concentration of IL - 18 binding protein alpha (IL - 18BPa) and IL - 18R beta with classical risk factors which include Anthropometric risk factors like Age, Central Obesity (CO) and body mass index (BMI), biochemical factors like Insulin , Insulin Resistance (IR), Fasting Blood Glucose ( FBG), Glycosylated Hemoglobin (HbA1c) and lipid profile. Besides, the study aims to studying their association with ABO blood system in the Iraqi Arab female patients with diabetes mellitus type 2 in order to figure out their effect with other factors as predictor or developer for diabetes mellitus type 2. The present study included 340 Iraqi Arab female patients newly diagnosed with DMT2 and 200 healthy individuals tested for phenotype and allele frequency. From those, 60 patients were tested for the immunological and chemical where compared to 28 healthy individuals. Immunological tests by using ELISA kits. While Chemical parameters using enzymatic and colorimetric methods. The study groups (patients and control) were divided into four sub groups (A, B, AB and O) according to the class of blood groups which were tested by using standard serological procedures. The present data showed : 1 - A significant increase in the serum level of IL - 18BPa in the patients of the present study, while there was no significant difference in the serum level of IL - 18R beta. 2 - A significant increased in mean of IL - 18BPa with increased age in both patients and control groups. While IL - 18R beta showed increase at group less than 40 year and decreased with increasing age in both patients and control group. Besides increased all other risk factors. 3 - The levels of both IL - 18BPa and IL - 18R beta were gradually non significantly increased the level in each patients group with an increased duration of disease and the same for IR. 4 - Patients with HbA1c less than 7.3% showed a significant increase in mean of IL - 18BPa and IL - 18R beta and decreased gradually with the increase of HbA1c. 5 - Patients and control subjects who carried blood group B had the highest mean of IL - 18BPa, Insulin and Insulin resistance. 6 - Patient subjects who carried blood group B and control subjects who carried blood group A showed the highest mean of IL - 18R beta. 7 - IL - 18BPa and IL - 18R beta act as independent risk factors in Iraqi Arab females with DMT2 which supported by the absence of significant correlation among IL - 18BPa, IL - 18R Beta and all others classical risk factors. 8 - A positive relation between levels of IL - 18BPa and risk of DMT2. This may be an indication of inflammation for newly diagnostic diabetic patients, while Serum levels of IL - 18R beta might be a predictor marker of disease progression. Further studies are needed to understanding the relationship between blood groups, inflammatory markers and risk of DMT2 in healthy population.

دراسات وراثية خلوية ومظهرية لثلاثة انواع من شبوطيات المياه العذبة العراقية (Barbus luteus, Cyprinion macrostomus, Chondrostoma regius) == Three Species of Iraqi Freshwater Cyprinidaes (Barbus Luteus, Cyprinion Macrostomus, Chondrostoma Regius)

Author name: اسماء سامي ابراهيم
Supervisor name: ندى عبد المجيد الانصاري | طه ياسين الدوري
General topic: Biology
Specific topic: zoology - Genetics
Degree: Doctorate
Language: Arabic
University location: Baghdad
First pages:
Abstract: هدفت الدراسة الحالية التعرف على الطراز الكروموسومي ونظام تحديد الجنس والتمييز بين الجنسين مظهريا في ثلاثة انواع من اسماك المياه العذبة العراقية ممثلة بسمكة الحمري Barbus luteus والبنيني كبير الفم macrostomus Cyprinionوالبلعوط الملوكي Chondrostoma regius | These present studies were carried out to determine the karyotypes & the system of sex determination between the two sexes as well as the sexual morphological characteristics of three species of Iraqi fresh water fish Barbus luteus, Cyprinion macrostomus, & Chondrostoma regius that were fished from Tigris river at Baghdad during 2005. The cytogenetic studies revealed that the chromosome number of Barbus luteus is 2n = 148 & the karyotype of males is (80m + 52sm + 16st) while the females has (80m + 51sm + 17st). The chromosome number of Cyprinion macrostomus is 2n = 50 the males has (6m + 24sm + 12st + 8t) while the females has (6m + 23sm + 13st + 8t). The chromosome number of Chondrostoma regius is 2n = 48 and the karyotype is (14m + 30sm + 4st). Sex chromosomes were identified in Barbus luteus & in Cypinion macrostomus for the first time , the sex is determined by ZZ / ZW system, the female was considered as heterogametic (ZW) while the male was considered as homogametic (ZZ) , & the chromosome Z was represented as submetacentric chromosome & chromosome W as small subtelocentric chromosome. In spite of the difference in their chromosome number , the ratio between red blood cell diameter & it's nucleus was approximated in these species. The differences among these species in both of the diameter of cell and the nucleus didn't match with the differences at the polyploidy levels or with the difference in chromosome number within the same level. The liver & intestine were recommended as new sources for chromosomal studies , because of the good ratio of dividing cells (71.4 % & 58.2 %) respectively from the whole dividing cells in kidney that was considered as a control. The crude aqueous extract of fresh convolvulus arvensis leaves showed good effect in arresting the cell division of fish at metaphase , the concentration (0.4, 0.6, 0.8, ) mg / gm arrested kidney cells of carp fish at the metaphase in ratios 71.1 % , 88.1 % , 94 % respectively from the colchicines treated kidney cells (control). Positive significance correlation was found (r = 0.9518) between the percentages of arrested cells at metaphase & the concentrations of crude extract. Comparison between the females and males of the three species revealed that in Barbus luteus the distance from head edge to the anal orifice & from head edge to the dorsal fin were larger in males than females. The females have dark colored fins with reddish ventral aspect of head & body in comparison with the males during the whole months of study. So , they were considered as permanently sexual dimorphism characteristics. But , during the spawning seasons (May & June ) a more brighting color of male with reddish coloration of both sides of body were noticed in comparison with female with a golden orange corneal coloration in both sexes. Thus, they were considered as temporary sexual dimorphism characteristics. In Cyprinion macrostomus the distance from the head edge to the anal orifice was larger in females than males. The colors of anal & caudal fins were darker in females than in males during the whole monthes of study.So , they were considered as perminantly sexual dimorphism characteristics. While, during the spawning seasons ( May , June , July & August) , the gill operculum was of orange color only in male that extend to the end of pectoral fin. So , this was considered as a temporary sexual dimorphism characteristic. In Chondrostoma regius the distance from the head edge to the dorsal fin & from the dorsal to caudal fin as well as the pectoral fin base length were larger in females than in males during the whole months of study it was considered that they are permanently sexual dimorphism characteristics. Differential characteristics were not noticed during the spawning season (February & March). The permanently sexual dimorphism & the general characteristics were not affected by the differences of standard length , weight , age & season , because they were observed in fishes of different lengths , weights & ages & during the months of study 2005. But , the seasons affect the development of temporary characteristics in Barbus luteus & Cyprinion macrostomus , because these characteristics were observed during the spawning season only. Aging of Barbus luteus was accompanied by growth increase as indicated by the mean of standard length during May , July, August, September & December & by the mean of weight during May , August, September, October & December. A positive correlation was found between standard length & weight during the months of study , with the exception of January. While aging of Cyprinion macrostomus & Chondrostoma regius was not accompanied by growth increase as indicated by the means of standard length & weight , but a positive correlation between standard length & weight was found during the studied months. Same growth type were observed in the three species of fish , it was allometric in two sexes.

دراسة وراثية خلوية وجزيئية للتاثير الوقائي للكركم في سمية العقار ميترونيدازول في الفئران البيض == Cytogenetic And Molecular Study of Protective Effect of Curcumin In Toxicity of Metronidazole In White Mice

Author name: فائق ابراهيم علي محمود
Supervisor name: عادل فوزي شهاب
General topic: Biology
Specific topic: zoology - Genetics
Degree: Doctorate
Language: Arabic
University location: Salahaddin
First pages:
Abstract: صممت الدراسة الحالية لغرض التعرف على التاثيرات السمية الوراثية والسمية الخلوية للعقار ميترونيدازول(MTZ) في الفئران البيض Balb/ C والتاثير الوقائي الذي توفره المعاملة المسبقة بالكركم(CUR) في سمية العقار ميترونيدازول. تم استخدام مؤشرات الوراثة الخلوية (النو | The current study was designed to identify the genotoxicity and cytotoxicity of Mitronidazole (MTZ) and protective effect of Curcumin (CUR) against the toxicity of MTZ in whit mice. Cytogenetic endpoints (micronuclei (MN) and mitotic chromosomes), Productive biomarkers (sperms), molecular cytogenetic biomarkers (comet assay), molecular endpoints (genomic and mitochondrial DNA concentration in liver and spleen and testis) and mutation assessment in mitochondrial DNA (mtDNA) of liver and testis by PCR and restriction pattern were used to evaluate genotoxicity, cytotoxicity of MTZ and protective effects of CUR in white Balb/Cmice. The study was conducted in the laboratories of college of education, college of science in Tikrit university and laboratories in the state company for drugs industry - Samarra - Iraq (SDI). Dosages of 30 and 50 mg/kb.b.wt. of MTZ were tested for genotoxic and cytotoxic and mutagenic effects in somatic and sperms of white mice. The dosages 4.5 and 10 mg/kgb.wt. of CUR were used to study the protective effects of CUR in genotoxicity and cytotoxicity and mutagenecity of MTZ. The current study showed that MTZ has genotoxic effect expressed by high frequency of micronuclei in polychromatic erythrocytes (PECs) which reached 15.5 ± 2.88 with the dosage 30 mg/kg.b.wt. and 21.31 ± 4.2 with the dosage 50mg/kg.b.wt. compared with 7.25 ± 1.88 in negative control. In the study of the protective effects of CUR against genotoxic and cytotoxic effects of MTZ, we observed that in mice treated with the dosage 4.5 mg/kg.b.wt of CUR for 9 days before the dosage 50 mg/kg.b.wt. of MTZ, There were significant p<0.05 decrease in MN frequency which reached 13.25 ± 4.19 compared with 21.31 ± 4.2 for mice treated with the dosage 50mg/kg.b.wt. of MTZ withoutCurcumin. The study showed that MTZ has cytotoxicity expressed by significant p<0.05 decrease in mitotic index (MI) which reached 0.010 in bone - marrow of the mice treated with 50 mg/kg.b.wt. compared with 0.023 in negative control. The dosage 4.5 mg/kg.b.wt. of CUR for 9 days before giving the dosage 50 mg/kg.b.wt. of MTZ resulted in returning of MI in bone - marrow 0.021 to its levels in negative control 0.023 compared with its value which reached 0.010 with the dosage 50 mg/kg.b.wt. of MTZ. The two dosages 30 and 50 mg/kg.b.wt. of MTZ caused significant p<0.05 decrease in total number of sperms which reached 2.4±2.4 and 1.5 ± 0.1 milions simultaneously compared with 6.7 ± 0.9 millions in negative control. More over there were significant p<0.05 decrease in motile sperms which reached 25.2 ± 11.0 and 14.6 ± 10.6 compared with 57.6 ± 8.0 of negative control. Significant p<0.01 decrease in the length of mid - piece and total length of sperm tail with the dosage 50 mg/kg.b.wt. of MTZ which reached 19.2 ± 6.0 and 69.0 ± 6.0 simultaneously compared with 31.6 ± 9.0 and 100.3 ± 15.0for negative control. Treating with the dosage 4.5 mg/kg.b.wt. of CUR for 9 day before the dosage 50 mg/kg.b.wt. of MTZ resulted in the return of mid - piece and total length of sperm tail which reached 29.5 ± 3.0 and 95.0 ± 5.0 close to the levels of negative control 31.06 ± 9.0 and 100.3 ± 15.0 simultaneously. Compared to 19.2 ± 6.0 for the length of mid - piece and 69.0 ± 6.0 for total length of sperm tail in mice treated with the dosage 50 mg/kg.b.wt. of MTZ without previous treatment with CUR. The study of damage levels in DNA with comet assay showed that MTZ induced significant p<0.01 levels of damage reached 45.4 ± 2.36 with the dosage 50mg/kg.b.wt. of MTZ in bone - marrow and 36.8 ± 0.58 in liver cells compared with 9.0 ± 2.12 and 5.2 ± 0.58 simultaneously in negative control. There were decrease occurred in the level of damage in DNA of bone - marrow and liver cells. However it did not return to its levels of negative control 9.0 ± 2.12 in bone - marrow and 5.2 ± 0.58 in liver cells. OTM values reached 26.4 ± 1.29 and 24.0 ± 1.14 in mice treated with the dosage 4.5 mg/kg.b.wt. of CUR for 9 days before the dosage 50 mg/kg.b.wt. of MTZ. However The values of OTM lower than its values in bone - marrow 45.4 ± 2.36 and 36.8 ± 1.66 in liver cells of mice treated with the dosage 50 mg/kg.b.wt. without CUR. In the molecular study, The results showed mutagenic effect of the dosage 50 mg/kg.b.wt. of MTZ in mice mtDNA expressed by novel restriction sites for Bam - HI and Hind - III in liver tissue and SauA3 in testis. The treatment of mice with the dosage 4.5mg/kg.b.wt. of CUR for 9 days before the dosage 50mg/kg.b.wt. of MTZ did not resulted in the inhibition of mutagenesis in mtDNA. In conclusion, results of this study showed that MTZ has genotoxic and cytotoxic effects in white mice and mutagenic effect in mice mtDNA. The dosage 4.5 mg/kg.b.wt.of Curcumin has protective effect against genotoxic and cytotoxic effects of MTZ. on the other hand the dosage 4.5 mg/kg.b.wt. of Curcumin has no protective effect against mutagenicity of MTZ in mice mtDNA.

تقييم التعبير الجيني للجين UGT1A1 لدى حديثي الولادة المصابين باليرقان == Assessment of UGT1A1 Gene Expression In Hyperbilirubinemic Neonates

Author name: حسين حميد حسن
Supervisor name: ظافرة جعفر عبد علي الفتلاوي | صباح نعمة محمد الفتلاوي
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: استخدمت في هذه الدراسة 125 عينة من حديثي الولادة لتقييم التعبير الجيني لل UGT1A1 والكشف عن المتغاير UGT1A1*28. استخدمت 85 عينة منها في تقييم التعبير الجيني لل UGT1A1, بينما استخدمت ال 40 عينة المتبقية للكشف عن المتغاير UGT1A1*28. اجريت الدراسة الحالية في | A cohort of 125 neonates enrolled in the present study 85 of which subjected to the UGT1A1 expression analysis and forty (40) independent subjects examined for UGT1A1*28 variant. The project was performed in the laboratory of molecular genetics in the collage of education for women, TSB, UCB, BG and ELISA were performed in ATHOP, Assader teaching hospital and Annajaf private laboratory in the period from may to October 2013. Analysis of the UGT1A1 gene expression showed considerable decrease in UGT1A1 expression with relative risk 1.46. Analysis of regression of UGT1A1 mean against severity showed significant inverse correlation between severity of NH and mean UGT1A1 level (r = - 0.99, p = 0.03). Results showed that UGT1A1 expression in males significantly lower than females. Analysis of UGT1A1*28 showed that 80% of the hyperbilirubinemic neonates were positive while 20% were UGT1A1*28 negative. Concluding that there are a considerable proportion of neonates in our community not expressing UGT1A1 enzyme; therefore, they are a risk group for kernicterus. And must be registered and followed up because they are at risk of carcinogenicity, therefore, irinotecan (an anti cancer drug) and similar compounds toxicity. Expression of UGT1A1gene is higher in female than in male neonates, while neonatal jaundice is less severe in female than in male neonates. Eighty percent of jaundiced neonates are UGT1A1*28 mutants. Defective UGT1A1 is the main underlying cause of NJ in our community.

دراسة بعض متغيرات الخطوط الجلدية للبنان ومجاميع الدم لذكور اطفال التوحد

Author name: اسراء جعفر صادق السعدي
Supervisor name: نصر فرحان عبد الله
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: Arabic
University location: Baghdad
First pages:

دراسة بعض المؤشرات الوراثية الخلوية لتاثير مادة الكلوروفورم على الفار الابيض Mus musculus L == A Study On Some Cytogenetic Indices For The Effect of Chloroform On The White Mouse Mus Musculus L

Author name: عمر رحيم خلف
Supervisor name: عادل فوزي شهاب
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: يستخدم الكلوروفورم على نطاق واسع في العديد من الصناعات مثل صناعة الورق والمطاط ومطافئ الحريق ومستحضرات التجميل ومواد التخدير. كما يستخدم كمذيب عضوي للعديد من المواد في المختبرات التعليمية والصناعية. وينتج الكلوروفورم من العمليات الايضية التي تقوم بها بعض | Chloroform is widely used in many industries, such as paper, elastic, fire extinguisher, cosmetics and anesthetic materials industries. It also used as an organic dissolvent for many materials in industrial and education laboratories. Chloroform is produced by The metabolism process of living organisms such as macroalgae and some fungus of highly organic matter soil. It can also be produced by process of water cholornation. Because of chloroform is widely used, and few studies achieved on cytogenetic level. Therefore The present study was conducted to identify The effects of chloroform on cytogenetic characteritis in laboratory mice.This study includes : 1 - A Study on Some Cytogenetic Indices For The Effect of Chloroform on The White Mouse.2 - Sperm abnormalities of male mice.The results of The present study showed that The chloroform has the ability to motivate chromosomal aberrations which were represented by (ring chromosom, chromatid break, and centromore break) in somatic cells in both males and females mice and in germ cells in males, The results revealed significant differences in The chromatid break and ring chromosome of both male and females mice, which injected with two concentrations (0, 025 & 0, 05 mg/kg) when compared with control group. It was found That there were asignificant differences in centromore break of females, injected with two concentrations of chloroform in comparison with control group, while non significant were differences observed in The males.Also the present study showed there were significant differences in The chromosomal aberration in The germ cells of males mice, injected with two concentrations (0, 025 & 0, 05 mg/kg) when compared with controlled group.The concentration (0, 05 mg/kg) was more influent in comparison, with The concentration (0, 025mg/kg). It was found that 30 days of injection is more influent in comparison with 15 day of injection, and males are more influenced than females.The present study recorded a significant differences in creating micronuclei in both male and female mice, injected by chloroform with a concentrations (0, 025 & 0, 05 mg/kg)in compared with control group.The result also revealed that The chloroform cause a significant decrease in The mitotic index of The somatic cells in both males and females, morever significant decrease in The mitotic germ cells of The injected males mice was found.An increase in The Blast index factor in The somatic cell in both male and female mice, injected by chloroform when compared with control group was similar result was recoded rgarding The Blast Index factor of The males germ cells. Regarding The sperm abnormality, the result showed significant differences in The sperm abnormality of males injected by chloroform with two concentrations (0, 025 & 0, 05 mg/kg) in comparison with The control group. The concentration (0, 05 mg/kg) was more influent than The concentration (0, 025mg/kg). Besides and the period 30 days of injection was more influent than the period 15 days.

تقييم العلاقة بين T1, M1) )) GST لدى نساء عراقيات عربيات مصابات بداء السكري من النوع الثاني وامراض الشرايين القلبية == Evaluation Of The Association Of Glutathione S - Transferase GST (T1,M1)Genetic Polymorphism In Iraqi Arab Females With Type 2 Diabetes Mellitus And Coronary Artery Diseases

Author name: مروة محي الدين محمود العبيدي
Supervisor name: بتول علي شهاب | عصام نوري سلمان
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: English
University location: Baghdad
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دراسة تعدد الاشكال الوراثية للجينين MTHFR وMTR ومرضى تشوهات القلب الولادية في محافظة ذي قار == genes with Congenital heart defects Study polymorphism of MTHFR & MTR patients in Thi-Qar province

Author name: وديان كاطع وشيل الفرطوسي
Supervisor name: حسن ريسان الركابي
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: Arabic
University location: Dhi Qar
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صفات الخطوط الجلدية والاجسام الكروماتينية الجنسية في الاناث العقم == Characteristics of Dermatoglyphics and Sex Chromatin in Infertile Females

Author name: رشا عباس عزيز النعيمي
Supervisor name: نصر فرحان عبد الله | سعيدة علي الانصاري
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: Arabic
University location: Baghdad
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اثر مستخلصي عرق السوس وثمرة الكاكي في تثبيط الانحرافات الكروموسومية الناتجة من المعاملة بمادة كبريتيت الصوديوم في خلايا اللبائن == The Effect of G. Glabra and D. Kaki fruit extracts in inhibition the chromosomal aberration resulted from The treatment of Sodium sulphte material in mammalian cells

Author name: رحيم فاضل حسين مرهون العامري
Supervisor name: عبد الامير ناصر غلوب الركابي | مؤيد صبري شوكت
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:

تاثير الفينولات المتعددة المستخلصة من الشاي الاخضر Camellia sinensis في الخلايا الطبيعية والسرطانية داخل وخارج الجسم الحي == Influence of Polyphenols Extracts of Green Tea Camellia sinensis on The Normal and Cancer Cells Lines In Vivo and In Vitro

Author name: محفوظة عباس عمران
Supervisor name: غازي منعم عزيز | ناهي يوسف ياسين
General topic: Biology
Specific topic: zoology - Genetics
Degree: Doctorate
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:

دراسة الخطوط الجلدية وعتبات التذوق لمادة الـ PTC في حالات سوء اطباق الاسنان == A Study of Dermatoglyphics and PTC Tasting Thresholdes in Malocclusions

Author name: منذر حمزة راضي العزاوي
Supervisor name: نصر فرحان عبد الله
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: Arabic
University location: Baghdad
First pages:

دراسة جزيئية ومناعية نسجية كيميائية لسرطان المثانة في محافظة النجف == Molecular And Immunohistochemical Study Of Bladder Cancer In Al - Najaf Province

Author name: كرار سليم زايد الشبلي
Supervisor name: اسعد عبد الحمزة الجنابي | عبد الزهرة كاظم صفر علي
General topic: Biology
Specific topic: zoology - Genetics
Degree: Doctorate
Language: English
University location: Najaf
First pages:

دراسة العلاقة بين فقدان الجينين GSTM1 , GSTT1 والاصابة بمرض الاعتلال الكلوي السكري في محافظة ذي قار == The Study Of The Relationship Between The Null Genotype Of GSTM1 And GSTT1 Genes And Diabetic Nephropathy In Thi - Qar Province

Author name: هدى طاهر فيصل
Supervisor name: حسن ريسان الركابي | عدنان عيسى البدران
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: English
University location: Dhi Qar
First pages:

دراسة الاشكال المتعددة لجين MTHFR لدى مرضى داء الزرق في محافظة ذي قار

Author name: ظفر محمد جاسم الموسوي
Supervisor name: حسن ريسان الركابي
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

دراسة وراثية للبكتريا المكونة للغشاء الحيوي والمعزولة من اطقم وادوات تقويم الاسنان == Genetic Study Of Biofilm Forming Bacteria, Isolated From Denture And Orthodontic Devices

Author name: خلود عبد الكريم حسين
Supervisor name: مناف جودة عبد العباس
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: English
University location: Basrah
First pages:

دراسة وراثية جزيئية للبكتريا المرافقة لالتهاب الاذن الوسطى التقيحي المزمن و البلعوم الانفي في مدينة البصرة. == Molecular Genetic Study Of Bacteria Associated With Chronic Suppurative Otitis Media And Nasopharynx In Basrah City

Author name: الاء عبد الزهرة جماغ
Supervisor name: مناف جودة عبد العباس
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: English
University location: Basrah
First pages:

استنسال جين بروتين الصدمة الحرارية groEL) 60 لبكتريا Salmonella Typhimurium ودراسة تاثيره الوقائي ضد الخمج بها في فئران BALB/c == Cloning Of Heat Shock Protein 60 Gene (groEL) Of Salmonella Typhimurium And Study Its Protective Effect Against The Infection In BALB/c Mice

Author name: اسراء فريد سعيد
Supervisor name: غازي موسى عزيز | علي حسن ادحية
General topic: Biology
Specific topic: zoology - Genetics
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

التحري عن العلاقة ما بين تعدد النمط الوراثي للجين GSTT1,GSTM1 في الرجال المصابين بمرض السكري النوع الثاني == Detection Of The Association Of (GSTM1, GSTT1) Genetic Polymorphism And Diabetes Mellitus Type 2 In Men Patients

Author name: هدى عبد الكريم حسين السعدي
Supervisor name: بتول علي شهاب | عصام نوري سلمان
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: English
University location: Baghdad
First pages:

دراسة وراثية وانزيمية للاناث المصابات بداء الربو == Genetic and Enzymatic Study on Female Patients with Asthma

Author name: هبة حسين عزيز الهويدي
Supervisor name: نصر فرحان عبد الله
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: Arabic
University location: Baghdad
First pages:

دراسة وراثية خلوية لمرضى ابيضاض الدم الحاد والمزمن == A Cytogenetic Study on Patients with Acute and Chronic Leukemia

Author name: نور هاشم اسماعيل
Supervisor name: ندى عبد المجيد الانصاري | ناهي يوسف ياسين
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: Arabic
University location: Baghdad
First pages:

تاثير العصير الخام لثمرة الزيتون Olea europaea في بعض المعايير الوراثية الخلوية في الفئران البيض وفي بعض الخطوط السرطانية في الزجاج == Effect of Crude Juice of Olive (Olea europaea) in some Cytogenetic Parameters in Albino mice and in some Cancer Cell Lines in vitro

Author name: مها بركان عبد الرحمن العزاوي
Supervisor name: بتول على شهاب | سعد محمد الندا
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: Arabic
University location: Baghdad
First pages:

دراسة تصنيفية مظهرية ووراثية على حشرة ذبابة فاكهة البحر المتوسط Ceratitis capitata (Wiedemann 1824) (Diptera: Tephritidae) في بعض من محافظات وسط العراق == Taxonomic and Genetic study on Mediterranean fruit fly Ceratitis capitata (Wiedemann 1824) (Diptera:Tephritidae)in some of mid-Iraqi provinces

Author name: محمد مهدي جواد
Supervisor name: احسان عرفان حسين | حسن سعيد الاسدي
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: Arabic
University location: Baghdad
First pages:

التغيرات الوراثية الخلوية والجزيئية المرافقة لاورام الارومة النخاعية واورام البطانة العصبية في الاطفال == Cytogenetic And Molecular Alterations Associated With Medulloblastoma And Ependymoma In Children

Author name: لمى حسن علوان العبيدي
Supervisor name: ندى عبد المجيد الانصاري | ناهي يوسف ياسين
General topic: Biology
Specific topic: zoology - Genetics
Degree: Doctorate
Language: English
University location: Baghdad
First pages:
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