Author name:
هدى فالح سعد الساعدي
Supervisor name:
رياض كاظم موسى | طارق فرج شوكت
Abstract:
This study was undertaken at the poultry field of Animal ResourceDepartment \college of Agriculture \University of Basra in Jan.,28th toMar.,3th,2013 and in Oct.,28th to December,23th 2013 for the first and secondexperimentrespectively . The objective of this study was to evaluate the effectof hatching eggs injection with the some nutritious (Folic acid vitamins,Pyridoxine, Threonine amino acid) for the first experiment and choosing besttreatment from first experiment and injection with Arginine amino acid ,folicacid as well as the effect of feeding time on the performance and somephysiological traits of broiler chicks .In the first experiment ,825 hatching eggsof Ross 308 broiler were received from the hatchery .At 14th day of incubationstage ,eggs were tested and unfertile eggs were discarded ,the remainder eggswere divided into seven treatments and injected with 0.1 ml of solution in airsac position of the egg as follows : 1. First treatment : injection 100 eggs with 0.1ml of threonine solution (30μgconcentration per an egg).2. Second treatment : injection 100 eggs with 0.1 ml of b6 vitamin solution(100μg concentration per an egg)3. Thirdtreatment : injection 100 eggs with 0.1 ml of mix from L - threonine aminoacid plus b6 vitamin (30μgand 100μg) per an egg.4. Fourthtreatment : injection 100 eggs with 0.1 ml of Folic acid vitamin solution(250g) per of an egg.5. Fifthtreatment : injection 100 eggs with 0.1 ml of mix solution from Lthreonineamino acid plus Folic acid vitamin (30μg and 250 g) per an egg.6. Sixthtreatment : positiveControl : injection100 eggs with 0.1 mlSterile distilledwaterper egg.7. Seventhtreatment : negativeControl : 155 eggs were left withoutinjection.Hatching chicks of each treatment were distributed in to three replicate (15 Chicksfor each replicate) .Birds were reared up to five weeks of age.The results of first experiment painted out to : 1. There was significantly (P<0.05) proceeding for the treatment of egg injectionwith some nutrients on hatching percentage ,hatching chick weight ,bodyBweight ,body weight gain and weekly and Cumulative feed conversion ratio.whereas, this treatment showed less embryonic mortality percentage incomparison with other experimental treatments and There was significantly(P<0.05) increase in weekly and Cumulative feed intake of birds that werehatched from injected eggs with ( folic acid ,threonine amino acid) during thesecond ,fourth and fifth week were as ,the treatment of pyridoxine injectionshowed less mortality percentage compared to other experimental treatments .2. Treatment of folic acid and threonine and pyridoxine injection showedsignificant (P<0.05) increases in the dressingpercentage, weights of breast,thigh and edible viscera comparison with other treatments.3. There was significantly(P<0.05) increase in red blood cells number,hemoglobinconcentration package cells volume of broilers that were hatchedfrom egg injection with (folic acid plus threonine) at 3 and 5 weeks of agecompared to other treatment While , Treatment of Pyridoxine eggs injectionshowed highest mean of white blood cells at 3 weeks of age, whereas,treatment of Pyridoxine and folic acid eggs injection proceeding otherexperimental treatment in highest mean of white blood cells at 5 weeks of age .4. There was significantly(P<0.05) increase in total protein concentration,glucose ,Globulins, and less uric acid concentration and cholesterol in chicksblood serum of folic acid and threonine egg injection treatment at 3 and 5weeks of age in comparison with other treatment. Whereas, there were nosignificant effect of egg injection on triglycerides concentration and on T3 andT4 hormone in broiler blood serum.5. There was no significantly effect of egg injection with nutritive on low densityfatty proteins concentration whereas,the negativecontrol treatment showedhighest concentration of low and high proteins at 3 and 5 weeks of agecompared to other experimental treatments.6. T4 hormone concentration in hatchingbroiler blood serum of threonineinjection treatment increased significant (P<0.05) at 3 weeks of age ,whereas,T4 hormones concentration was higher respecting treatment of folic acid andthreonine injection at 5 weeks of age in comparison with other treatment.7. There was significantly (P<0.05) increase in phosphorus ,calcium and ferricconcentrations in blood serum of the hatchingbroilers of folic acid andthreonine egg injection treatment at 3 and 5 weeks of age in comparison withother treatments.C8. There was significantly (P<0.05) increase in the villus lengths cryptsdepth ofjejunum of the boilers Caracasrespecting Folicacid and Threonine injectiontreatment in comparison with other treatment.According to the firstexperiment results and choosing the best treatment of egginjection with nutritive, the second experiment was carried out .the treatmentof this experiment whereas follows : 1. Firsttreatment : hatching eggs injection with (30μg of threonine, 250g of folicacid) per an egg.2. Secondtreatment : hatching eggs injection with (5 μg of arginine) per an egg.3. Thirdtreatment : hatching eggs injection with (5 μg of arginine plus 250 goffolic acid) per an egg.4. Fourth treatment : the control treatment (withoutinjection).Hatching chicks of each treatment were divided in to groups each of threereplicates ( 12 Chicks for each replicates ) .firstgroups chicks were fed at thehatchery ,whereas,second groupschicks were fed after 24 hours at field. Chickswere reared from 1 day after hatching up to 5 weeks of age .the results of thesecond experiment pointed out to : 1. Treatments of injection with arginine and Folic acid and threonine plus folicacid proceeding significantly (P<0.05) in hatching percentage andhatchingchicksweight, whereas, This treatment showed less percentage of lateembryonic mortality in comparison with other treatments. Also, Broiler birdsof Arginine and Folic acid injection treatment showed significantly (P˂0.05)increase in body weight, weekly body again, weekly and Cumulativefeedintake,weekly and Cumulative feed conversion ratio and dressing percentage.Whereas, treatment with arginine and folic acid injection showed less totalmortality mean compared to other treatments. Also, there was significantly(P˂0.05) effect of feeding time on some productive traits. Treatments offeeding at the hatchery showed significant increase in body weight, bodyweight gain, feed intake, better feed conversion ratio and dressing percentagein comparison with treatments of feeding at field after 24 hours.2 - There was a significantly (P˂0.05) effect of interaction between eggs injectionwith nutritious and feeding time on body weight, body weight gain and feedconversion ratio. Treatment of folic acid arginine (hatchery feeding) showedDhighest body weight, body weight gain, better feed conversion ratio incomparison with the control treatment (Field Feeding after 24 hours).Treatment of arginine injection (HatcheryFeeding) was highest dressingpercentage, whereas there was no significant effect of interaction on feedintake and total mortality.3 - Treatment of threonine and folic acid injection showed a significantly (P˂0.05)increase in bird’s body fullness, gizzard weight and Fabricia gland. Whereas,treatment of arginine and folic acid injection showed a significantly (P˂0.05)increase in breast fullness liver and heart weight in comparison with othertreatments.4 - Feeding time affected significantly (P˂0.05) on some carcasses traits. Carcassesof feeding at hatchery treatment were better significantly in body fullness,breastfullness, and heart, liver, gizzard, and Fabricia gland and pancreasweights. Whereas, carcasses of feeding at field treatment were of high pad fatweight. There was no significant effect of interaction between injection andfeeding time on carcasses traits.5 - There was a significant (P˂0.05) increase in red and white blood cells numbers 3days and 5 weeks of age and in hemoglobin concentration at 5 weeks of agerespecting the treatment of arginine and folic acid in comparison with othertreatments. Control treatment exceeded in Heterophil cells\ lymphocyte cellspercentage at 3 and 5 weeks of age compared to treatments.6 - There was a significantly (P˂0.05) effect of feeding time on some cellular bloodcharacteristics. Treatment of the field feeding exceeded in red blood cellsnumbers, hemoglobin concentration and Heterophil cells\lymphocyte cellspercentage at 3 days and 5 weeks of age. Whereas, treatment of the hatcheryfeeding exceeded in white blood cells numbers compared to treatment of thefield feeding after 24 hours.There was no significantly (P˂0.05) effect ofinteraction between injection and feeding time on the cellular bloodcharacteristics at 3days of age, whereas this interaction affected significantly(P˂0.05) on the white blood numbers and Heterophil cells\lymphocyte cellspercentage at 5weeks of age.E7 - Treatment of arginine and folic acid injection exceeded significantly (P˂0.05)in serum total protein concentration at 3 days and in total proteinconcentration, uric acid and GPT, GOT enzymes in blood plasma at 5 weeksof age. Treatment of threonine and folic acid in comparison with othertreatments.8 - Feeding time affected significantly (P˂0.05) on biochemical bloodcharacteristics. Treatment of the hatchery feeding exceeded in glucoseconcentration and total protein at 3 days of age and in total protein concentration and uric acid at 5 weeks of age. Treatment of the field feeding exceeded in blood serum cholesterol concentration in comparison with thetreatment of hatchery feeding. There was no significant (P˂0.05) effect ofinteraction between injection and feeding time on the biochemical bloodcharacteristics at 5 weeks of age. 9 - There was a significant (P˂0.05) increase in villus Length and crypts Depth of the intestinal jejunum of comparison with of threonine and folic acid injection treatment in comparison with other treatments. Treatment of the hatchery feeding exceeded (P˂0.05) respecting those traits compared to the field eeding treatment. There was a significantly (P˂0.05) effect of interaction (TheHatchery Feeding) and feeding time villus, length and crypts Depths werehighest at 1 and 5 weeks of age.
