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دراسة وراثية جزيئية فسلجيه لحبلات العقم لدى الرجال في محافظة ذي قار == Moliceogentics - Physiological - Study Of Male Infertile In Thiqar Province

Author name: طالب كريدي عودة الحساني
Supervisor name: حسن ريسبن مابرك | ايناس صالح جواد
General topic: Biology
Specific topic: Microbiology - Genetics
Degree: Doctorate
Language: Arabic
University location: Dhi Qar
First pages:

دراسة مظهرية ونسجية وكيمياء نسجية للغدة الملحية والغدة الزمكية في دجاج الماء Moorhen Gallinula chloropus == Histological and Histochemical Study of The Salt Gland and The Uropygial Gland in Moorhen (Gallinula chloropus)

Author name: عبير كريم غانم المنصوري
Supervisor name: ســـتار عبود فارس | علاء عبد الخالق حسين
General topic: Biology
Specific topic: Zoology - Histology
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

دراسة نسجية وفوقية لتاثير نوعين من اشعة الليزر على الجلد والخصى في الفئران المختبرية Mus musculus Balb/ c == Histological and ultrastructure study of the effect two types of laser radiation on skin and testis of laboratory mice Mus musculus Balb /c

Author name: نشات علي سعود الخفاجي
Supervisor name: ستار عبود فارس
General topic: Biology
Specific topic: Zoology - Histology
Degree: Doctorate
Language: Arabic
University location: Dhi Qar
First pages:

دراسة مناعية لعلاقة اضطربات المناعة الذاتية للغدة الدرقية مع فيروس ابشتاين بار وفيروس التهاب الكبد الوبائي سي

Author name: الحسنى عادل منصور الخياط
Supervisor name: ماجدة غازي مكطوف
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

دراسة كيمياء نسجيه مناعية للكشف عن وجود فايروس الورم الحليمي البشري(Human Papilloma Virus) وفايروس ابشتاين - بار(Epstein - Barr Virus) في سرطان الثدي في نساء محافظة ذي قار == Immunohistochemical study to detection of Human Papilloma and Epstein - Barr viruses of Breast Cancer in women Thi - Qar providence

Author name: نور فالح شريف السعداوي
Supervisor name: ستار عبود فارس | حميد نعيم موسى
General topic: Biology
Specific topic: Zoology - Histology
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

دراسة تعدد الاشكال لجين CYP19A1 وعلاقته ببعض التغيرات الفسلجية لمصابات متلازمة المبيض المتعدد الكيسات في محافظة ذي قار == A study of the polymorphism of CYP19A1 gene and its relation to some physiological changes in the incidence of polycystic ovarian syndrome in Thi - Qar governorate

Author name: دعاء حازم محمد
Supervisor name: حسن ريسان مبارك | ايناس صالح جواد
General topic: Biology
Specific topic: Microbiology - Genetics
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

دراسـة تعدد الاشكال الوراثية للجين CYP1A1 في العاملين في مصفى ذي قار النفطي == A study of the polymorphisms CYP1A1 gene of the Thi - Qar refinery oil workers

Author name: صــلاح مهــدي مطــر البدري
Supervisor name: حسن ريسان الركابي
General topic: Biology
Specific topic: Microbiology - Genetics
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

دراسة بعض التاثيرات الفسيولوجية والنسجية لمضادي الاكتئاب Fluoxetine) و Sertraline) في الجرذان المختبرية البيض norvegicus == Study of some physiological and histological effects of antidepressants (Fluoxetine and Sertraline) in albino laboratory rats norvegicus Rattus

Author name: زمن صبحي مدلول
Supervisor name: ستار عبود فارس | علي مانع حسين
General topic: Biology
Specific topic: Zoology - Histology
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

دراسـة فسـلجية لتاثيــر حامضي الجبرلين و السالسليك في النمو الخـضـري والمحتوى الكيـميائي لنـبات الشــوفان Avena sativa L. صــنـف (شفاء == Physiological study on the effect of Gibberellin and Salicylic acid on vegetative growth Characteristics and Chemical content of oat plant (Avena sativa L.) variety Shifaa

Author name: زين العابدين جعفر عيسى
Supervisor name: صباح ناهي ناصر السعيدي
General topic: Biology
Specific topic: Plant - Physiology
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

تعدد الاشكال الوراثية للجينات GSTT1, GSTM1 و CYP2C19 كعوامل خطورة لامراض الدم الخبيثة لمرضى جنوب العراق == Analysis of CYP2C19, GSTT1and GSTM1 Genes Polymorphisms As Risk Factors for Hematological Malignancy in Southern Iraqi Patients

Author name: دموع قاسم محمد الخفاجي
Supervisor name: حسن ريسان الركابي | رافد عادل الخالدي
General topic: Biology
Specific topic: Microbiology - Genetics
Degree: Master
Language: English
University location: Dhi Qar
First pages:

الكشف الجزيئي لبعض عوامل الضراوة من بكتريا المكورات المسبحية القيحية و المكورات العنقودية الذهبية المعزولة من المرضى المصابين ب?لتهاب البلعوم واللوزتين في محافظة ذي قار/ العراق == Molecular detection of some virulence factors of Streptococcus pyogenes and Staphylococcus aureus isolated from Pharyngitis and Tonsillitis patients in Thi - Qar province/Iraq

Author name: بيداء رسول داخل
Supervisor name: سعد سلمان هميم
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Dhi Qar
First pages:

العزل و الكشف الجزيئي لبعض جينات الضراوة والبلازميدات لبكتريا السالمونيلا من الاطفال المصابين بالاسهال في محافظة ذي قار /العراق == Isolation and Molecular Detection of Some Virulence Genes and Plasmids of Salmonella enterica from Diarrheal Children in Thi - Qar Province/Iraq

Author name: زمن كريم حنان
Supervisor name: منال بادي صالح | عزت حسين مزعل
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Dhi Qar
First pages:

التنميط المصلي والكشف الجزيئي لبعض جينات المقاومة لجرثومة الاشريشيا القولونية Escherichia coli المعزولة من الاطفال المصابين باخماج المجاري البولية في مدينة الناصرية == Serotyping and molecular detection of some resistance genes of Escherichia coli isolated from urinary tract infected children in Nasiriyah City

Author name: وداد سمير جعاز الازيرجاوي
Supervisor name: سعد سلمان هميم
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

دراسة تاثير الاشعة الكهرومغناطيسية للهاتف النقال في بعض المعايير الفسلجية والتغيرات النسجية في الجرذان المختبرية البيض norvegicus Rattus == Study of Effect Electromagnetic Radiations of Mobile Phone in Some Physiological Parameters and Histological Changes in White Laboratory Rats Rattus norvegicus

Author name: فاطمة عزيز مهدي البدري
Supervisor name: ستار عبود فارس
General topic: Biology
Specific topic: Zoology - Histology
Degree: Doctorate
Language: Arabic
University location: Dhi Qar
First pages:

التحري عن بعض المعايير المناعية وتعدد الطرز الوراثية لجين IL - 13 في مرضى الربو في محافظة ذي قار == Investigation of some Immunological parameters and polymorphism of IL - 13 gene in patients of asthma in Thi - Qar province

Author name: هند حسين صالح الطائي
Supervisor name: علي نعيم سلمان
General topic: Biology
Specific topic: Zoology - Immunology
Degree: Master
Language: English
University location: Dhi Qar
First pages:
Abstract: Asthma is a chronic inflammatory disease of the airways in which many cells and cellular elements play a role. Causes recurrent episodes of wheezing, breathlessness, chest tightness, and coughing. These episodes cause airflow obstruction, often reversible either spontaneously or with treatment. The current study was conducted at the Labs of college of Education for pure science and Al - Hussein Teaching Hospital in Thi - Qar province, during the period from October 2014 to May 2015. The study aimed to investigate role of polymorphism of IL - 13 gene in patients with asthma and to evaluate their immune status by measuring the levels of some immunological parameter immunoglobulins ( IgE) by enzyme - linked immunosorbent assay (ELISA) and (IgG, IgM ) by single redial immune diffusion (SRID) and measure the levels of interleukins (IL - 6, IL - 17) in the serum using enzyme - linked immunosorbent assay (ELISA), the study included test phagocytic cells on phagocytosis (coefficient of phagocytosis) and the total and differential count of white blood cells .A total of 100 patients with bronchial asthma (68 females) and (32 males) whose age lies between 17 - 62 years have enrolled in this study. DNA was extracted and RFLP - PCR was performed by using primers specific for the gene IL - 13, the results showed the presence of mutations in a48 sample out of 100 patients with asthma at site 1112C/T after using restriction enzyme BstUI. the statistical analysis showed correlation between the occurrence of the disease and the emergence of mutation (C / T) in the promoter region of IL - 13 gene when compared with the healthy control in population of Thi - Qar province .When it was compared the patient group with healthy control group there was a high significant increase (P ≤ 0.001) in serum IgE, IgG of patients with asthma compared with healthy control group. And no significant difference in serum IgM of patients with asthma compared with healthy control group. Decrease coefficientphagocytosis was significantly (P ≤ 0.001) in all patients with asthma compared with healthy control group. Levels of interleukin (IL - 6, IL - 17) measured in serum of 60 a sample of patients with asthma, and in the serum of 20 samples from healthy control group. The results showed a significant increase (P ≤ 0.001) in the levels of these interleukins in the serum of patients with asthma compared to the control group.Also there was increased in the rate of counting the total of WBCs and differential cell lymphocytes, eosinophil, neutrophil, basophil, and monocyte (P ≤ 0.001) in patients with asthma compared with the healthy people in the control group.

دراسة جزيئية ونسيجية مناعية للنساء المصابات بمرض سرطان الثدي في محافظة ذي قار,العراق == Molecular and Immunohistochemical Study in Women with Breast Cancer in Thi - Qar Province

Author name: هبة لفتة عبد الغالبي
Supervisor name: منال بادي صالح | مها شاكر حسن
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: English
University location: Dhi Qar
First pages:
Abstract: Breast cancer is a complex and multifactorial disease resulting in abnormal cell growth that leads to malignant tumor formation.In this study we focus on mutations of breast cancer susceptibility genes 1 and 2 (BRCA1 and BRCA2), immunohistochemical evaluation of estrogen, progesterone, HER - 2 receptors, ABO blood groups , white blood cells and clinicopathological parameter ( age, breast feeding, family history and tumor site) .Eighty five blood samples were taken from patients who attended Al - Hussain teaching hospital and oncology unit in Al - Habboby hospital during the period (from August /2014 to April /2015) , fifty blood samples were collected from healthy women as a control group who attended Al - Hussain teaching hospital and divided into groups according to age, family history, age at first brith, breast feeding. For histopathological and immunohistochemical analysis fifty tissue samples were collected from patients with breast cancer who were undergoing surgical resection (mastectomy). Blood samples were obtained by venepuncture , using a 5 ml disposable syringe , and they were divided into three aliquots, which were 1 ml, 2 ml and 2ml, for collect serum, genetic studies and hematological parameters (leucocycte count and ABO blood groups) study. The tissue samples were collected from the 50 patients with breast cancer for histopathological diagnosis and for Immunohistochemical analysis (estrogen receptor, progesterone receptor and here2/nue) The total patients were divided according to their age in to five groups (30 - 39), (40 - 49), (50 - 59), (60 - 69) and (≥70 years). results of present study revealed that a highest incidence of breast cancer (41%) occur in the age oup between 40 - 49 years, there is a significant difference among patients groups (P ≤ 0.01).The results of our study showed that (77) of patients lactating and from those according to period of lactation we found that the highest percentage of patients (37.6%) were lactating for period between (18 - 22months) and the lowest percentage (10.38%) lactating for period between (1 - 6 month). Family history of breast cancer indicates a strong association with risk of developing breast cancer. The result of the present study revealed that the family history positive in 31 cases (36.47%), and 54 cases (63.52%) had negative family history of breast cancer.The results of present study showed that (41.5%) had first birth at age (20 - 29years), (23.37%) had their first birth in age group (15 - 19 years) and (30 - 39 years), and (9.09%) had first birth at age (≥40 years).Regarding the tumor site, we found that most of patients (50.5%) the tumor mass was located in the right side and (38.8%) cases the tumor mass were located in the left side.The DNA was extracted from blood samples by using Accupower® genomic DNA extraction kit. Each concentration and purity of all DNA samples have been measured by Nanodrop. Samples with a purity ranged from 1.2 to 1.8 have been enrolled in this study for the molecular detection of BRCA1/2 gene mutation in patients and control groups. Detection of BRCA1/2 gene mutation by multiplex PCR revealed that (185 del AG mutation in BRCA1 gene) was detected in 6 patients (7.05%) out of 85 cases with breast cancer, and (5382 ins C) mutation in the same gene was detected in 2 patients (2.35%). Regarding (6174 del T) mutation in BRCA2 gene was detected in 3 patients (3.52%). While this mutation was not detected in the control groups.Histopathologically, carcinoma was divided into 44 cases (88%) ductal carcinoma {from which 42 cases (84%) were invasive ductal carcinoma, and 2 cases (4%) were comedocarcinoma}, and 6 cases (12%) cases invasive lobular carcinoma. Results revealed that 2 cases (4%) were stage I, 13 cases (26%) were stage II, 24 cases (48%) were stage III and 11 cases (22%) were stage IV, also our results showed that the 2 cases (4%) were grade I, 23 cases (46%) were grade II and 25 cases (50%) were grade III. The results of immunohistochemical analysis for breast cancer samples showed that Estrogen receptors were present in 36 cases (72%), and 14 cases (28%) were negative, while Progesterone receptors detected in 27 cases (54%), and 23 cases (46%) were negative, on the other hand the expression of HER - 2/neu showed positive results in 28 cases (56%), and 22 cases (44%) were negative.Regarding the ABO blood groups, we found that most of the cases carry blood group A (40 cases, 47.05 %), and the lowest frequency was for blood group AB (7 cases, 8.23%).The results of the present study showed that leukocytes count, lymphocytes count, monocytes count, neutrophils count and basophiles count were lower in patients than the control group, with a significant difference (p value ≤ o.o1). While the eosinophils count was significantly higher in patients than the controls (p value ≤ o.o1).

تقييم كفاءة بعض المرشحات كبدائل في معالجة مياه الصرف الصحي واعادة استخدامها في ري نبات الذره الصفرا ء Zea mays L == Evaluation Efficiency For Some Alternatives Filters in Sewage Treatment and Reusing in Irrigation Plant Zea mays L

Author name: منى محمد نجیب
Supervisor name: حسين يوسف خلف الركابي
General topic: Biology
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: recently Iraq where suffer from several crisis including diminished quantity and deteriorated quality of water resources, specially in southern provinces. This study was designed to show the extent of using non - traditional water resources such as sewage after its treatment with local alternative chemicals and using the resulting water in agriculture sector. Samples were collected from the terminal tank of waste water station in Al Nasiriyah ( Alhindia station) according to the criteria of United States Environment Protection Agency (USEPA, 1977) regarding the sewage properties including pH, EC, total dissolved solid(TDS), total suspended solids( TSS), CO2, Mg+2, Ca+2, Na+, CaCO3, Cl - , PO4 - , NO3 - , chemical oxygen demand ( COD), biological oxygen demand (BOD5). Moreover, we easured three trace elements ( Pb, Ni, Cd) as well as sodium adsorption ratio( SAR) and biological properties and bacterial contamination with fecal coliform bacteria. Physicochemical analysis of both sewage and tab water was performed. It was found that pH values were 7.37 and 8 for sewage and tab water , respectively. While the electrical conductivity for tab water was 8.01 ds / m compared with that of sewage water which was only 1.76 ds / m . TDS, TSS and CO2 for both sewage and tab water were (3780, 723) and ( 800 , 320) and (5.41, 1.23) mg/ L respectively. Results also showed that sewage water contain high concentrations of cations and anions as compared to tab water. Magnesium concentration was 469.42mg/L in sewage water and 100.1mg/L in tab water while calcium was 581.2mg/L in tab water and 60.8 in sewage water. Sodium concentration was 662.86 mg/L in sewage water and 186.3mg/L in tab water. On the other hand, water hardness and alkalinity were (3300 , 280) mg/L for sewage water and ( 2.40, 66.49) mg/L for tab water respectively .Results of this study also showed an increase in the concentrations of some ions in crude sewage water with respect to tab water. Concentrations ofchloride,phosphate,sulphate and nitrate in sewage water sample were 2496.31 Mg/ L,1.6 Mg / L , 1976 Mg / L ,3.67 Mg / L and for tab water were 226.8,0.7,108.48,0.6 Mg/L, respectively. Fecal coliform number was 2600 cell/100 ml in sewage sample and only 6 cell/ 100 ml in tab water. BOD5 andCOD values were 18, 167 Mg/ L for sewage water and 2.0, 6.0 Mg/L for tab water respectively Heavy elements also found to be elevated in sewage with respect to tab water , where the concentrations of Pb,Ni& Cd in sewage sample were 2.8, 0.8 & 0.004 Mg / L , while they were 0.09, 0.001 &undetected in tab water, respectively. On the other hand, sodium adsorption ratio SAR in sewage samples was 7 and in tab water was only 0.48.Laboratory experiments dealt with the following : 1 - It was used sand filters as a primary processing for waste water to remove pollutants prior to pass it through used filter upon incubating it for 14 days . 2 - in this study , it was selected four types of filters to remove contaminants and improve the quality of water is (water plant Ceratophyllum demersum, wood sawyer , , coal and Cladophora glomerta ). As it was used as filters either aprimary step in processing waste water through passing these waters as aby - pass step these waters on sand filter was used in this studay .3 - chemical , physical and biological characteristics of waste waters were studied upon passing it on the under - studying filters and also estimated the efficiency of removal Removal efficiency (%) of the components. 4 - Used of Some international Standerds and Iraqi specifications to evaluate the quality of water befor and after to pass it on filters for irrigation purposes. Some of criteria Such as (Kloke, 1980; WHO, 1995; US EPA, 1996; European Society, 1996 and Awasthi, 2000. ) were used to in the evaluation of soil contamination with heavy metals.This study reached to the following results : .The study concluded accrediting wooden sawyer + sand and water plant in recycling and reforming waste water and its capacity to remove pollutants, evaluation of quality of waste water and the treated water after passing through the filters under studay results indicated that all types of water inclusive the class (C4S1 ) which means high salinity water with afew of sodium element according to the classification of Laboratory US salinity. and water to cause toxicity problem in the study, and showed It results that candidates contributed to the removal of pathogenic bacteria from the treated water, and irrigation with waters passed through ceratophyllum dermersum L and sand filter leading treatment gave the highest dry to plant maize and two shoot and root weight, Result of statistical analysis showed that soils characteristics had a significant effect on of dry weight, concentration and quantity absorbed heavy elements in sections shoot and root to plant maize, was not for irrigation with treated wastewater impact on soil pollution with heavy metals and the results showed that the soils environmentally safe after relying on the Organization standards (WHO) and the American Environmental Protection Agency (US EPA).

توصيف جرثومة Enterobacter sakazakii المعزولة من مصادر مختلفة في محافظة ذي قار == Characterization of Enterobacter sakazakii Isolated from various sources at Thi - Qar Province

Author name: مروى حسن نصير الصافي
Supervisor name: يحيى عبد الرضا عباس | راجي طعمة ناصر
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Dhi Qar
First pages:
Abstract: هدفت هذه الدراسة الى الكشف عن بكتريا Enterobacter sakazakii في بيئات مختلفة شملت عينات غذائية وبيئية وسريرية . اذ جمعت 310 عينة خلال الفترة من ايلول 2012 ولغاية تموز 2013.اظهرت نتائج الزرع الاولي على وسطVRBAواكار الماكونكي بعد التشخيص بالاعتماد على الصفات الزرعية والكيموحيوية وتوكيد التشخيص باستعمال العدة API 20E. ان 22 (7.09%) من اصل 310 عينة اعطت نموا لبكتريا Enterobacter كالاتي 9/80(11.2%) من بيئة المستشفيات ,12/180(6.6%) حليب اطفال,1/50(2%)من حالات الاسهال . توزعت العزلات بعد التشخيص المختبري على الانواع التالية (15/22) 68.1% Enterobacter cloacae,(4/22)18.1% Enterobacter sakazakii و3/22))13.6% للنوع Enterobacter aminogenus. اختبرت مقاومة عزلات Enterobacter sakazakii الاربع ضد 20 مضادا من اصناف مختلفة وقد اظهرت النتائج ا ن جميع العزلات كانت مقاومة على الاقل لست من المضادات قيد الاختبار.اختبرت قدرة بكتريا Enterobacter sakazakii على انتاج انزيمات البيتا لاكتيميز واسعة الطيف واظهرت العزلات عدم قدرتها على الانتاج واستخدمت طريقة القرص المصاحب لمعرفة قابلية العزلات على انتاج انزيمات الميتالو بيتا لاكتاميز.درست قدرة البكتريا على تكوين الغشاء الحيوي واظهرت النتائج ان 2/4(50%) من العزلات لها قدرة على انتاج الغشاء الحيوي.كما د رست قدرة البكتريا على مقاومة التاثير القاتل للمصل وظهرت العزلات كلها موجبة بينما درست قدرة البكتريا على تلازن كريات الدم الحمراء لوحظت تحت المجهر واظهرت النتائج ان 3/4(75%)من العزلات كانت موجبة.اختبرت قدرة البكتريا على انتاج السموم المعوية باستخدام الفئران الرضيعة وظهرت كل العزلات منتجة له وقد ظهرت على الفئران الرضيعة بالسموم المعوية اعراض سريريه حادة مثل انخفاض النشاط والحركة البطيئة وعلامات ضعف مع تغيرات في المعدة وخسارة التركيب النسيجي للغشاء المخاطي والغدد وظهور بؤر للخلايا المتحللة وزيادة في سمك الجدار مع تقرحات في النسيج اما التغيرات المرضية في الامعاء فهي تغيرات غير مخصصة تمثلت بتقطعات في النسيج الطلائي وتقرحه وفقدان لشكل النسيج والغدد. | This study was aimed for detection occurrence of Enterobacter sakazakii in stool specimens, infant formula and hospitals environments. A total of 310 samples (180 infant formula, 50 stool samples and 80 hospital environment swabs ), during the period from September 2012 to July 2013 were collected. Growth on Violet redbile agar and MacConkey agar was identified by cultural and biochemical tests and confermated by API 20 E system which revealed that : Only 22/310(7.09%)gave positive growth for Enterobacter spp. as following : 9/80 (11.2%) environmental swabs , 12/180(6.6%) infant formula and 1/50 (2%) stool samples. Diagnosis of the isolates appeared that E. cloacae was (15 ⁄ 22) while E . sakazakii (4 ⁄ 22) and E. aminogenus (3/22). Four E. sakazakii isolates were screened for their antibiotic resistance against 20 antibiotics of different classes using Kirby - Bauer disk diffusion method. The results showed that all the tested isolates were resistant to at least 6 antibiotics of which they were tested . In the present study four E. sakazakii isolates where screened for potential ESBLs production, they showed 3 (75%) isolates were potential ESBLs production ,but conformation of ESBLs production was detected by double disk synergism test and appeared that all the isolates were unproductive . Combined disk diffusion method was used for detection of metallo - β - lactamase - producing isolates, they were emplace each of imipenem and ceftazidame antibiotics with and without EDTA on Mueller - Hinton agar, because EDTA working to inhibit of metallo - β - lactamase. The results showed that the presence of EDTA does not affect the diameter of inhibition zone for the four isolates. Biofilm production was detected by spectrophotometric method .The four isolates were tested and appeared that 2(50%) were produced biofilm. The resistance of bacterial isolates to serum bactericidal activity was tested by the turbidmetric assay . All isolates were appeared able to resist to serum bactericidal activity, while Agglutination of erythrocytes was tested under the microscope and showed 3/4(75%) were positive. In the present study E. sakazakii were evaluated enterotoxin production. All the isolates showed positive for enterotoxin production. Results of treating suckling mice with enterotoxin showed acute clinical signs appeared depression, slow movement and signs of weakness. Significant macroscopic finding showed in mice treated with enterotoxin the stomach was distended and enlarged with thick corrugated mucosa but no significant changes in intestine. Pathological changes in the stomach appeared as loss of normal architecture of mucosa and glands, multiple foci of degenerative cells, reactive epithelial surface with thickening wall, pseudo - metaplasic changes and superficial epithelial injury. The intestinal changes showed nonspecific pattern of damage to surface of epithelium .Complete loss of architecture of glands and mucosal epithelial , superficial epithelial discontinuity , ulcer and non - specific reactive epithelial surface.

عزل وتشخيص الفطريات الجلدية من المرضى المصابين بداء السعفة في محافظة ذي قار ودراسة تاثير بعض العوامل في نمو تلك الفطريات == Isolation and identification of dermatophytes from patients with tinea in Thi - Qar Province and studying some factors effecting on their growth

Author name: سوزان خالد كاظم العسكري
Supervisor name: ياس خضير عباس | محمد حسين مشهد
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: تضمنت الدراسة الحالية عزل وتشخيص بعض انواع الفطريات الجلدية المسببة لداء السعفة ((Tinea من المرضى المراجعين للاستشارية الجلدية في مستشفى الامام الحسين التعليمي في محافظة ذي قار , اذ جمعت 180عينة مرضية شملت 128عينة قشور جلدية و24عينة شعر و28 عينة اظافر من مرضى يعانون من داء السعفة (( Tinea للفترة من 13/8/2014 الى 1/1/ 2015 . بلغ عدد العينات المرضية الموجبة للزرع 110 عينة وبنسبة 61.1 % , اذ شكل عدد اصابات الاناث 74 وبنسبة 67.27 % , اما اصابات الذكور فقد بلغت 36 وبنسبة 32.73 % . بينت الدراسة ان الاصابة بسعفة الجسم (Tinea corporis) احتلت المرتبة الاولى من بين الاصابات وبنسبة 57.27 % ثم سعفة الراس ((Tinea capitis 17.27 % , ثم سعفة القدم 12.73 (Tinea pedis)% وشكلت سعفة الاظافر (Tinea unguium) بنسبة 9.09% , ثم سعفة اللحية (Tinea barbae)بنسبة 3.64 % . شخصت الفطريات الجلدية المسببة لداء السعفة باستخدام عدد من الاختبارات التشخيصية وقد وجد ان الفطر Microsporum canis اكثرها ظهورا وبنسبة 36.36 % يليه الفطــر Trichophyton tonsurans وT. verrucosum وM. gypseum وM. fulvum وبنسبـــة 27.27 % , 17.27 % , 12.73 % و6.36 % على التوالي . بلغت اعلى نسب للاصابة بسعفة الراس والجسم والقدم والاظافر في الاناث , في حين كانت اعلى نسبة للاصابة بسعفة اللحية في الذكور . وكان لعامل العمر تاثير في نسبة الاصابة , اذ احتلت الفئة العمرية (10≥) النسبة الاعلى في سعفة الراس , بينما كانت الفئة العمرية ( 31 - 40) النسبة الاعلى لاصابة سعفة الجسم والقدم واللحية اما سعفة الاظافر فكانت الفئة العمرية ( 21 - 30) النسبة الاعلى . بلغت نسبة الفطريات المفضلة للحيوان Zoophilic)) 53.6 % تلتها الفطريات الجلدية المفضلة للانسان (Anthropophilic) بنسبة 27.2 % , ثم الفطريات الجلدية المفضلة للتربة (Geophilic) وبنسبة 19.2 % . كما تناولت الدراسة تاثير بعض العوامل البيئية مثل نوع الوسط الغذائي ودرجة الحرارة والاس الهيدروجيني ومدة الحضن في نمو ثلاثة انواع من الفطريات الجلدية التابعة لجنس Microsporum وهي Microsporum canis وM. gypseum وM. fulvum . وقد اظهرت نتائج الدراسة ان افضل وسط غذائي لنمو الانواع الفطرية المعزولة هو Agar Sabouraud's Dextrose مقارنة مع وسطي Potato Dextrose Agar وCorn Meal Dextrose Agar , وكانت درجة الحرارة المثلى لنمو الفطريات الجلدية هي 25 م̊ . في حين كان الاس الهيدروجيني (pH = 6) للوسط الزرعي SDA هو الافضل في تنمية الفطريات الجلدية . كما اوضحت الدراسة زيادة معدلات اقطار المستعمرات الفطرية الجلدية النامية على الوسط الزرعي SDA عند pH = 6ودرجة حرارة 25 م̊ بزيادة مدة الحضن . كما تم استخــــدام تقنيــــة تفاعـــــل سلسلــــــة البلمرة ( PCR ) باستخــــدام البادئ ITS1 - 2FW - ITS1 - 2FV لتشخيص بعض انواع الجنس Microsporum . كما شملت الدراسة الكشف عن المواد الفعالة في المستخلص الكحولي لاوراق نبات اكليل الجبل ( Rosemarinus officinalis) اذ وجد ان المستخلص يحتوي على الفينولات والقلويدات والصابونينات والعفصيات والتانينات والراتنجات والكلايكوسيدات ولا يحتوي على الكومارينات. وقد تبين ان لهذا المستخلص قدرة تثبيطية ضد الفطريات الجلدية وبلغ اعلى تثبيط عند التركيز 200 ملغم / مل . وقد تباين التركيز المثبط الادنى (MIC) حسب النوع الفطري , فكان اقل تركيز مثبط ادنى للمستخلص الكحولي لاوراق نبات اكليل الجبل 25 ملغم / مل تجاه معظم الانواع الفطرية الجلدية , كما وجد ان المضاد الفطري Griseofulvin اكثر فعالية من المضاد الفطري Ketoconazole في تثبيط الفطريات الجلدية . | A study of isolation and identification of dermatophytes in Thi - Qar Province was conducted . A total of 180 clinical samples ( 128 skin scraping samples , 24 hair samples and 28 nails samples ) were collected from patients suffering Tinea and were consulting in Dermatology unit in AL - Hussein Teaching Hospital during 13/ 8/ 2014 to 1 / 1 / 2015 . 110 samples were positive for culture with a percentage of 61.1 % . Females infections were 74 cases ( 67.27 %) ,while male infections were 36 cases (32.73 %) . The study showed that Tinea corporis was the most Tinea infections with a percentage of 57.27 % . Tinea capitis , Tinea pedis , Tinea unguium and Tinea barbae had a percentage of 17.27 % , 12.73 % , 9.09 % and 3.64 % respectively . The isolated fungal species causing Tinea were Microsporum canis , Trichophyton tonsurans , T. verrucosum ,M. gypseum and M. fulvum with a percentage occurrence of 36.36 % , 27.27 % , 17.27 % , 12.73 % and 6.36 % respectively . Tinea capitis , Tinea corporis , Tinea pedis and Tinea unguium were high in females , while in males , Tinea barbae was the highest . Infection with Tinea was affected with patients age , so patients under 10 years old had tinea capitis more than others ; Tinea corporis , Tinea pedis , and Tinea barbae had the highest infection percentage in ages between 31 - 40 years . Tinea unguium had the highest infection percentage in patients with ages between 21 - 30 years . Zoophilic dermatophytes had the percentage of 53.6 % , Anthropophilic dermatophytes and Geophilic dermatophytes presented as 27.2 % and 19.2 % respectively . The effects of some environmental factors such as culture media , temperature , pH and incubation period on M. canis , M. gypseum . M. fulvum was studied .The best culture media for these dermatophytes was Sabouraud's Dextrose Agar comparing with Potato Dextrose Agar and Corn Meal Dextrose Agar. The optimum growth temperature was 25 oC , while pH = 6 was the best for culturing dermatophytes on SDA and 25 oC . Polymerase Chain Reaction (PCR ) with primer ITS1 - 2 FW - ITS1 - 2 RV was used to confirm the identification of Microsporum species . The alcoholic extract of Rosemarinus officinalis contained phenols , alkaloids , resins , tannins and glycosides , saponins . The extract had the highest inhibition activity against the isolated fungi at the concentration of 200 mg / ml . The minimal inhibitory concentration ( MIC ) of the alcoholic extract of Rosemarinus officinalis was 25 mg / ml . Griseofulvin was more active than Ketoconazole against dermatophytes

الكشف عن جينات البيتالاكتاميز واسعة الطيف لبكتريا الكلبسيلا المعزولة من عينات سريرية وبيئية في محافظة ذي - قار == Detection of ESBLs in Klebsiella spp. Isolated from Clinical and Environmental Samples at Thi - Qar Province

Author name: زينب محمد عيدي اللامي
Supervisor name: يحيى عبد الرضا عباس
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Dhi Qar
First pages:
Abstract: This study aimed for isolation and identification of Klebsiella spp.from clinical and environmental samples and detection the prevalence of some extended spectrum β - lactamases (ESBLs) genes (bla CTX - M, bla CTXM1) in Klebsiella spp. Samples collected were included clinical samples ( Burns , wounds , urinary tract infections, respiratory tract infections, otitis media and vaginal infections ) and hospitals environments. A total of 554 (468 clinical and 86 environmental samples) during the period from October 2013 to March 2014 were collected. Growth on blood agar, MacConkey agar and E.M.B agar was identified by cultural, morphological and biochemical tests and confermated by API 20 E system which revealed that : 45 ∕ 554 ( 8,1%) gave positive growth for Klebsiella spp. as following : 2 ∕ 48 (4.1 %) fromburns , 7∕ 40 (17.5 %) from wounds, 29 ∕315 ( 9.2%) from urinary tract infections, 4 ∕ 25 (16%) from respiratory tract infections, 3 ∕86 (3,4%) from hospitals environments.All the 45 isolates of Klebsiella spp. were screened for their antibiotic resistance against 15 antibiotics of different classes using Kirby - Bauer disk diffusion method. The results showed that all the tested isolates were resistant to at least 6 antibiotics of which they were tested, hence the isolates were considered to be multidrug resistant.The results showed that all of the isolates were found to be resistant to β - lactam antibiotic (ampicillin and amoxicillin) Disk approximation test and not production of ESBLs by these isolates.Combined disk diffusion method was used for detection of metallo - β - lactamase - producing isolates , it was emplace each of imipenem and antibiotic with and without EDTA on Mueller - Hinton agar , because EDTA working to inhibit of metallo - β - lactamase . The results showed that there was no effect for EDTA on the diameter of inhibition zone in all isolates.All the 45 isolates were submitted to molecular detection of some ESBLs genes (bla CTX - M , bla CTX - M1) using PCR technique. The results showed that only 28 ( 62,2%) isolates were carried ) blaCTX - M or blaCTXM1) and 21 isolates were carried both genes (blaCTX - M and blaCTXM1).

تاثير حليب الابل في بعض المعايير الفسيولوجية في اناث الجرذان المختبرية Rattus norvegicus المعامله بالبنزين == The Effect of Camel's Milk in Some Physiological Parameters of Female Rats (Rattus norvegicus) Treated with Benzene

Author name: زينب محمد عباس الغزي
Supervisor name: خالد كاطع الفرطوسي | محمد عجة عودة
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Master
Language: English
University location: Dhi Qar
First pages:
Abstract: The present study was conducted in Department of Biology, College of Sciences, University of Thi - Qar, Iraq to investigate the effect of camel's milk on some physiological parameters of female rats (Rattus norvegicus) treated with benzene which included (weight of body and internal organs, hematological and biochemical parameters). The study was included 48 female rats divided into 8 equal groups, 6 for each group, the control group (A) which treated orally with (5 ml /kg) of physiological solution (NaCl 0.9%) for 4 months, camel's milk group (B) which treated orally with (5ml/kg) camel's milk for 4 months, benzene group(1) (C) which injected (I.P.) with (0.2ml/kg) of benzene by two doses for each week for four months, benzene group(2) (D) which injected (I.P.) with (0.4ml/kg) of benzene by two doses for each week for four months, protection group (E) which injected with (0.2ml/kg) of benzene and treated with (5ml/kg) of raw camel's milk for four months, protection group (F) which injected with (0.4ml/kg) of benzene and treated with (5ml/kg) of raw camel's milk for four months, treatment group (G) which injected with (0.2ml/kg) of benzene and after the end of duration of injection treated with (5ml/kg) of raw camel's milk daily for 1 months, treatment group (H) which treated with (0.2ml/kg) of benzene and after the end of duration of injection treated with (5ml/kg) of raw camel's milk daily for one month, and identified the animal weights at the beginning and end of the experiment and after the end of the period experiment all rats were weight organs (heart, liver, kidneys and spleen) and took blood samples to measure the level of malondialdehyde MDA, Ceruloplasmin CP, Total Cholesterol TC, Triglyceride TG, height density lipoproteins HDL, low density lipoproteins LDL, very low density lipoproteins VLDL and the levels of ALT, AST and ALP. The current study showed significant decrease at (P≤ 0.05) in the weight of (body, heart and kidney), and the acidophil, RBCs, SUMMARY

دراسة تشخيصية ووبائية لطفيلي المقوسة الكوندية لطلبة جامعة ذي قار باستعمال تقنيتي الاليزا Toxoplasma gondii وتفاعل سلسلة البلمرة ذو الوقت الحقيقي == Diagnostic and Epidemiological Study of Toxoplasma gondii for Students of Thi - Qar University by ELISA and Real - Time PCR Techniques

Author name: رباب علي شناوه الموسوي
Supervisor name: بسعاد عقرب العبودي | منال بادي التميمي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: English
University location: Dhi Qar
First pages:
Abstract: Toxoplasma gondii is a unique intracellular parasite, which infects a large proportion of the world, population, but clinically uncommonly causes significant disease. The present study was performed for the first time in Thi - Qar province to estimate the prevalence of toxoplasmosis among university students. Blood samples were collected from 319 (111 males and 208 females) apparently healthy students, they have ages between (18 - 42) years attended from different colleges of Thi - Qar university (students of morning and evening study), during the period from October 2013 to April 2014.Enzyme linked Immunosorbant Assay (ELISA), was used to evaluate the presence of anti - Toxoplasma IgM and IgG antibodies and detection of B1 gene of T.gondii DNA by Real - Time Polymerase Chain Reaction (RT - PCR).The results indicated that 21.94% of students were exposed to positive for anti - Toxoplasma antibodies, 5.33% of them had IgM, 11.91% had IgG, and 4.70% had both IgM and IgG. Statistically, they were significant difference between them.There were a significant difference between males and females in total rate but not significant of antibodies pattern in them, which recorded 7.52% of males and 14.42% of females were positive for anti - Toxoplasma antibodies.The study demonstrated that no significant difference in infection percentages between colleges where students studied, although the highest seropositivity students was 47.83% in education sports college whereas it did not score any infection with toxoplasmosis in College of Education for Pure Science .The current study also showed that the highest positive percentage with T.gondii of male students was 28.57% in College of Engineering while the highest percentage with T.gondii of female students was 30.43% in College of sports education.The present study revealed that the acute infection of toxoplasmosis which presented IgM and both IgM and IgG was high at small age youngstudents (18 - 22) years while the chronic infection which presented IgG was high at old students (38 - 42) years.The present study demonstrated that seroprevalence of toxoplasmosis was significantly associated with close contact with cats. Although more students in the positive sample group eating undercooked meat but not significant difference between those 36.13% and other students who did not have this habit 13.5% has been discovered.The results showed the highest positive percentage samples at middle socioeconomic level was 24.10% while the lowest percentage at good socioeconomic level was 19.60% .The results showed that the highest positive percentage samples 31.0% at Refaee district whereas the lowest percentage was 19.78% as noticed at Naserya district.The present study demonstrated that seroprevalence of toxoplasmosis was significantly associated with presence of clinical symptoms (fever, headache, malaise, influenza) that may have a relationship with toxoplasmosis of infected students when compared with not presence clinical symptoms of those (p<0.05) they recorded 18.57% and 81.43% respectively.The present study revealed an association between blood group system and Toxoplasma infection with highest prevalence among samples of blood group A+ 30.65% and lowest prevalence in samples of blood group B+ 17.44% .The current study evolutes the rate of some blood parameters, in infected and non infected students, there was no significant difference observed in mean hemoglobin level (Hb), total count of leukocytes (WBCs), neutrophilis, lymphocytes, monocytes and basophilis numbers in both student . While significant differences were observed in mean of eosinophils number of infected students was more than number of non infected students.Moreover, mean of lymphocytes number recorded significant differences between infected and non infected females only.Besides the serological diagnosis of T.gondii, Real - Time PCR (RT - PCR) technique was used to confirm the infection with T.gondii by detection B1 gene of T.gondii DNA in the blood of students. Out of 319 students only 6.26% showed positive for toxoplasmosis among those 2.50% were males and 3.76% were females. The positive result in RT - PCR analysis were distributed on the patterns of the anti - Toxoplasma antibodies by ELISA test , Any positive blood samples with IgM, IgG and both IgM and IgG respectively have not been found whereas 20 positive cases of no anti - Toxoplasma antibodies.Real - Time PCR test in student's blood has advantages in detection of recent or active toxoplasmosis

التغيرات الفصلية والموقعية لتراكيز بعض العناصر النزرة في الرواسب والمياه والنبات المائي الشمبلان Ceratophyllum demersum في نهر الفرات عند قضاء سوق الشيوخ جنوب العراق == Seasonal and Reginal changes in concentration of some trace elements in sediment, water, and aquatic plant(Ceratophyllum demersum) in the Euphrates River at Suq Alshuyoukh districtsouthern of Iraq.

Author name: نور كاظم خير الله
Supervisor name: باسم يوسف الخفاجي
General topic: Biology
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: The current study was performed to get the knowledge of seasonal and Reginal changes in concentration of some trace elements(Zn, Pb , Ni and Co) in sediment, water and aquatic plant (Ceratophyllum demersum) in the Euphrates River at Suq _ Alshuyoukh district, south of Iraq . The study was include some of the chemical and physical properaties for water (air and water temperature,turbidity, electrical conductivity,salinity,dissolved oxygen , biochemical oxygen dimand, pH,nitrate,phosphat also total organic carbon and sediment texture). The samples were collected from the river on seasonaly from Autumn 2015 to summer 2016. There are three stations were selected for the study , the first station was near by the tourist Alfadhliyah bridge, afertile farmland on both sides of the river. The second station was in Suq_Alshuyokh , it's away from the first station about 15 km , it's a center of boats which used for fishing ,agriculture lands and homes. The third station was located in stream of ALkermashiya which lieson snuffa_marsh (apart of the Hammar marsh) ,It's away from the second station about 11 km and it's near by villages and fertile farmland. The results of study showed that air temperature was ranged between (15.8 - 49.5)° C and water temperature was ranged between (13.8 - 48.7 ) °C . The highest temperature recorded in the third location during Summer. Turbidity concentrations ranged between (5.7 - 15.6) NTU. The electrical conductivity are ranging between (3920 - 8336) Ms/cm , and salinity of water was low (Oligohaline) ranging between (2.50 - 5.33) ppt and recorded the highest concentrations in the third station . the highest value of pH was (8.9) in the secondstation and the lowest value was (7.3) in the first location while the Dissolved Oxygen concentrations ranged between (3.1 - 8.45) mg/L. The biological oxygen demand (BOD5) was ranged between (0.35 - 4.5) mg /L. As for concentration of nitrates was ranged from (0.02 - 6.33) mg/L. and phosphate ranged between (0.01 - 5.8) mg/L. the total organic carbon as percentage was ranged between (0.48 - 3.1) %.while sediment texture was mixed from sand ,silt,clay,the percentage for the mentiond compenents was,(50%,20% and 30%)in station 1,(40%,10%,50%)for station 2 and (10%,20% and 70%) for station3Atomic absorption spectrofotometer was used to estimate four trace elements (Zn,Pb, Co, and Ni) .The concentrations of the mentionel elements in water dissolved phase were (2.86,5.87,1.63,0.51) μg /L,while their concentrations in particulate phase were (12.48,28.21,17.74,1.08) μg /gm dry weight respectively. In sediment the concentrations averages oftrace elements were (142.73,127.70,176.82,29.24) μg /gm dry weight respectively.In aquatic plant (Ceratophylum demersum) the concentrations averages of trace elements were (123.32,117.54,157.54,27.90) μg /gm dry weight respectively . It concluded that the concentration of studied elements in sediment more than their concentration in water (dissolved and particulate) phase and more than their concentration in plant (Ceratophyllum demersum), also all concentrations of trace elements were in the international acceptable values.

دراسة مصلية ومناعية لداء المقوسات في مرضى القلب == Serological and Immunological Study of Toxoplasmosis in Heart Disease Patients

Author name: حيدر صباح عبد الحسين
Supervisor name: بسعاد عقرب العبودي | احمد حسن محمد
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: English
University location: Dhi Qar
First pages:
Abstract: Toxoplasmosis is the general term for infection and disease in human and animal caused by a parasite called Toxoplasma gondii, this disease is prevalent among different categories of people , Heart disease patients are one of them. Because of the dormancy status of T. gondii, there is ambiguity and variation in the immunity of patients. This study aimed to investigate the immune status of heart disease patients infected with toxoplasmosis, to detect the prospective relationship between heart disease patients and T. gondii, and investigate the relationship between toxoplasmosis and risk factors which included gender, age, blood group, contact with cats, and habitation.One hundred fifty samples of blood had been collected at period from August 2016 till November 2016 from patients with heart disease, they lying or visit Heart Medical Centre in Thi - Qar province. According to gender, the patients divide in to (81 males and 69 females) with age ranging 18 to 82 years. Also one hundred samples of blood had been collected from apparently healthy persons as negative control ,dividing according to gender in to ( 55 males and 45 females) with age ranging 19 to 56 years . All samples of both subjects had been tested by ELISA technique to detect anti - Toxoplasma Abs (IgG & IgM) . While the positive samples with toxoplasmosis were tested to detect the level of interleukins (IL - 10, IL - 12 and IL - 17).The overall of seropositivity of anti - Toxoplasma Abs that scored by ELISA in the current study was 44% in heart disease patients are higher than those in apparently healthy controls 19%, and there was a significant difference between them. There is a significant difference between male and female, in the seropositivity of anti - Toxoplasma Abs that scored in controls, in male was higher than those in female. Presence of antiSummary toxoplasmosis Abs in all types of heart disease patients with significant difference among them, and the highest percentage of anti - toxoplasmosis Abs in Mitral stenosis( MS) was 55.8% , and the lower value was 30.3% in Myocardiopathy.There are significant differences between the age groups and the percentage of anti - Toxoplasma Abs in heart disease patients with highest value was 63.2% at age group of 61 - 75 years, and the lowest one was 26.3% at age group of 15 - 30 years. While no significant differences between the age groups and toxoplasmosis in apparently healthy controls with highest value was 19.5% at age group of 31 - 45 years, and the lowest one was 8.6% at age group of 15 - 30 years.The results demonstrated that seroprevalence of toxoplasmosis was significantly associated with close contact with cats. The percentage of seroprevalence anti - Toxoplasma Abs in heart disease patients and controls inhabited rural area was 52.5% and 32% are higher than those inhabited urban area was 34.3% and 6% respectively. Some blood parameters were estimated of both subjects infected and non - infected with toxoplasmosis. There was high significant difference at p≤0.05 observed in mean ratio of eosinophil’s number between infected and not infected subjects with toxoplasmosis in both subjects. While there was no significant differences in mean ratio of total count of WBCs. Moreover, mean of lymphocytes number recorded significant differences betweeninfected and non - infected heart disease patients only. The blood group phenotypes of the infected heart disease patients and controls highlight the role in toxoplasmosis infection and the AB blood group characterized by the highest significant percentage of infection. The results of interleukins appeared high mean levels of IL - 12 82.85 pg/ml in heart disease patients infected with toxoplasmosis when compared with control 60.97 pg/ml, and there was significant difference between them. While there was no significant difference in IL - 10 and IL - 17 in both patients and controls. The results also showed decreased level of IL - 17 in patients with heart disease. The increased level of IL - 12 in patient with heart disease infected with toxoplasmosis indicate to the activity of cell mediated immunity in those patients and may be lead to provoke an inflammatory status in the heart valves and muscle then worse heart disease to be sever and need complicated treatments

دراسة وبائية فسلجية لطفيلي المقوسة الكونيدية Toxoplasma gondii لمرضى الغسيل الكلوي والسطان في محافظة ذي قار العراق == Epidemiological and Physiological Study of Toxoplasma gondii for Patients with Renal Dialysis and Cancer in Thi - Qar Province - Iraq

Author name: نور كاظم مطر الركابي
Supervisor name: بسعاد عقرب العبودي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: The current study was conducted in Thi - Qar province to investigate of Toxoplasma gondii prevalence among renal dialysis and cancer patients by using Enzyme Linked Immunosorbent Assay test and also the study determined of some factors that may affected that may affected the prevalence of infection, such as age, sex, marital status, place of residence and the contact with cats and a number of chemical dosing that exposed to it cancer patient or the number of times the dialysis for renal dialysis patients.One hundred blood samples were collected from renal dialysis patient and one hundred samples others were collected from cancer patient from two hospital AL - Hussein Hospital and AL - Habboubi Hospital for the period from the beginning October 2015 to the end of the month of March 2016 and prepared a questionnaire form for each person to get their information, as well as the collection of 50 blood samples from (healthy) as control group . .The results of the current study showed that the prevalence of toxoplasmosis in Thi - - Qar province in both renal dialysis and cancer patients and control group were 28% and 36% and 24% respectively. recorded results of Enzyme Linked Immunosorbent Assay ELISA test results for a positive antibody IgG in both renal dialysis and cancer patients and control group 25% , 30% , 22% respectively. As for the IgM antibody study did not record any positive result in both categories of the study (dialysis and cancer) only control group was 2%. The result of IgG & IgM together in renal dialysis and cancer and control group 3%, 6%, 0% respectively. .The highest infection prevalence were detected in renal dialysis in the age group (40 - 49 years) amounted (26.67%) and less infection in the age group (60> years) with percentage of 23.08% . As for cancer patients, the highest infection prevalence in the age group (50 - 59 years) was 53.85% and were less infection in the age group (30 - 39 years) amountin 14.29%. .The current study recorded there is a significant differences at level of P <0.05 in infection prevalence accoriding to gender for renal dialysis patients as it was infection prevalence in males 35.18%, while in the females the infection prevalence was 19.56%. As for cancer patients the study recorded significant differences P <0.05 where the infection prevalence in females were highest compared to males where it was 38.59% and 32.55% respectivelyThe study did not show any significant differences P> 0.05 for the infection prevalence according to contact with cats in renal dialysis patients but the study found significant differences between married and unmarried where the infection prevalence among married 25.84%, while the unmarried 45.45% .The infection prevalence were in the City highest 29.27% compared to countryside which amounted to 22.22%, the study found that the increase in the number of times the dialysis increased incidence of infection prevalence it was 25.53% for patients who conducted the dialysis treatment more than six times and 16.66% for patients who have less than six times. In cancer patients, the study observed significant differences P <0.05 for the contact with cats and it was highest infection prevalence in the city amounted to 45.21% compared to countryside 11.11%. and recorded a high infection rates at married 37.90% either at unmarried did not show they have no infection by parasite. For factor chemical dosing show high infection rates in patients who have been exposed to more than one dose of a chemical 37.63% As for those who were not exposed to any chemical dose was ratios have 14.29% .and wasblood type B + higher infection in renal dialysis patients 40.74% either in the cancer patients were O+ highest amounted to 58.62 %The results of the blood Parameters which conducted for infected males by toxoplasmosis and non - infected had not noticed a change in the hemoglobin for both categories. As total count and differential white blood cells the study did not record significant differences in the renal dialysis patients except neutrophil blood cell was observed height in infected males than non - infected . as for Cancer group was a decrease in the total number and differential white blood cells except acidophilus did not record any change in its level. As the females the study results were that there was no significant difference for both renal dialysis and cancer patient except Lymphocyte found decline in infected than non - infected for cancer patients .showed the results of the current study for standards biochemical that there are no significant differences P <0.05 for renal dialysis and cancer patient in the case of urea, creatinine, the study do not found any effect of toxoplasmosis on the rates of both urea and creatinine. As albumin was observed to high at dialysis patients and low at cancer patients at infected with toxoplasmosis compared to non - infected either control group the study did not reported significant differences for urea and creatinine and albumin. either liver function the study did not find change in the level of liver enzymes (AST.ALT) in dialysis and cancer patients and control group (healthy) .The presnt study recorded there is no significant differences in lipid profile (HDL, LDL, VLDL, Cholestrol, Triglyceride) at infected and non - infected in renal dialysis goup either cancer patients was observed high (HDL, VLDL, Cholestrol, Triglyceride
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