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استنسال وتعبير جين مستقبل الهيموغلوبين Leishmania tropica في بكتريا Eschrerichia coli == Cloning and Expression of Leishmania tropica Haemoglobin Receptor (HbR) in Escherichia coli

Author name: مي رضا جعفر
Supervisor name: ياسين اسماعيل عمران | محمد محمود فرحان
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:

انتاج وتنقية وامراضية بروتييز العزلة المطفرة لبكتريا Pseudomonas aeruginosa المعزولة من عينات مرضية == Production, purification and pathogenicity of protease from a mutant of Pseudomonas aeruginosa isolated from clinical samples

Author name: حسين عواد حسين
Supervisor name: بشرى هندي صالح | نضال سهيل زبار
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:

اثر الرنا الميكروي والمدورات الخلوية والاجهاد التاكسدي على انتاج الصبغات في مرضى عراقيين مصابين بالبهاق == Impact of MicroRNAs, Cytokines and Oxidative Stress on Pigmentary Process in Iraqi Patients with Vitiligo

Author name: ياسر وسام عيسى الامرائي
Supervisor name: شهلاء مهدي صالح
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:

العزل والتعبير الجيني لجينات BabA في العزلات السريرية للبكتيريا الملوية البوابية Helicobacter pylori والمعالجة بالمعزز الحيوي لخميرة Saccharomyces boulardii == Isolation and Gene expression of BabA Gene in Clinical Isolates of Helicobacter pylori Treated with Probiotic Yeast Saccharomyces boulardii

Author name: عامر علي حمادي
Supervisor name: محسن هاشم رسن | حسن محمود موسى
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: English
University location: Baghdad
First pages:

التنميط المتسلسل متعدد المواقع لبكتريا اشرشيا القولون متعددة المقاومة للمضادات الحياتية والمعزولة من التهاب المجاري البولية == Multi - locus Sequence Typing of Multi - drug Resistant Escherichia coli Isolated from Urinary Tract Infections

Author name: اية احمد كمال العامري
Supervisor name: عصام سلمان العزاوي | ظفر نجم العكيلي
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:

انتاج, تنقية وتوصيف انزيم الاسباراجينيز من العصيات المعزولة محليا == Production, Purification and Characterization of L - asparaginase from Locally Isolated Bacillus sp.

Author name: نور رياض ابراهيم
Supervisor name: نضال سهيل زبار
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:

التحري الجزيئي عن العناصر المنتقلة المشفرة للمقاومة لمضادات الحياة في عزلات سريرية لبكتريا Streptococcus pneumoniae == Molecular Detection of Transposable Elements Conferring to Antibiotic Resistance in Clinical Isolates of Streptococcus pneumoniae

Author name: دعاء حمود ادعير ال منشد
Supervisor name: حميد مجيد جاسم
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:

التشخيص الجزيئي لبكتريا السل المقاومة للادوية المتعددة في مدينة بغداد == Molecular Diagnosis of Multiple Drug Resistance Mycobacterium tuberculosis in Baghdad City

Author name: حيدر خالد مصطفى
Supervisor name: محسن هاشم ريسان | محم عبد الاخوة الفحام
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: English
University location: Baghdad
First pages:

التاثير السمي الخلوي والسمي الجيني لـ Lantadene A المنقى المحمل بواسطة توليف دقائق الذهب النانوية على خطوط الخلايا السرطانية == Cytotoxic and Genotoxic Effect of Purified Lantadene (A) Loaded by Synthesized Gold Nanoparticles on Tumor Cell Lines

Author name: نور ضياء جعفر
Supervisor name: علي زيد الصفار | عماد عبد الحسين يوسف
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:

الكشف عن الطفرات الموجودة في جين مستقبلات الاستروجين الفا وبيتا لنساء عراقيات مصابات بسرطان الثدي == Detection of Estrogen Receptor Alpha and Beta Gene Mutations in Iraqi Women with Breast Cancer

Author name: سحر مدحت حسين
Supervisor name: هيفاء هادي حساني | محسن هاشم رسن
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: English
University location: Baghdad
First pages:

تحضير وتوصيف عقار الدوكسوروبيسين المحمول في الدقائق النانوية البوليمرية PCL - PEG - PCL وتاثيره السمي الخلوي على خط خلايا سرطان الثدي MCF - 7 == Preparation and Characterization of Doxorubicin Loaded Tri - Block Copolymer (PCL - PEG - PCL) and its Cytotoxic Effect on Breast Cancer Cell Line MCF - 7

Author name: رندة محمد ضاحي علوان
Supervisor name: حميد مجيد جاسم | ميساء جاسب حاتم
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: English
University location: Baghdad
First pages:

Molecular and Genetic Study on G6PD in a Sample of Iraqi Patients

Author name: Hadeel Mohammed Khalaf
Supervisor name: Rehab Subhi Ramadhan
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: English
University location: Baghdad
First pages:

التحري عن قابلية نبات السذاب لتحمل المعادن الثقيلة خارج وداخل الجسم الحي == Investigating Heavy Metals Tolerance in Ruta graveolens In vivo and In vitro

Author name: زينب محمود لطيف
Supervisor name: كاظم محمد ابراهيم | عبد الواحد شمخي جابر
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:

دراسة تاثير المعززات الحيوية على فعالية النسبية لانزيمي الهيلورونديز والستربيتوكاينيز المنتجة من عزلات محلية لبكتريا المكورات المسبحية القيحية == Effect of Probiotics on The Relative Activity of Hyaluronidase and Streptokinase from Locally Isolated Streptococcus pyogenes

Author name: ياسمين ثائر احمد
Supervisor name: عبد الواحد باقر الشيباني | نضال سهيل زبار
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:

انتاج وتنقية البروتييز من طافرة لبكتريا Aeromonas hydrophila المعزولة محليا

Author name: ميس طالب عبد الله
Supervisor name: اسماء علي حسين | حميد مجيد جاسم
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:

دراسة مقارنة على معاملة التهاب قدم مريض السكري بحامض الخليك ومضاد الريفامبسين والمعالجات الحيوية == A Comparative Study on Treatment of Diabetic Foot Infection by Acetic Acid, Rifampicin and Probiotics

Author name: نورهان صبيح رسن
Supervisor name: عبد الواحد باقر عبد الرضا الشيباني | صادق عباس المختار
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: English
University location: Baghdad
First pages:

العلاقة بين اضطراب الغدة الدرقية والاصابة ببكتريا Helicobacter pylori == The Relationship between Thyroid Disorders and Helicobacter pylori Infection

Author name: سارة طالب كاظم المفرجي
Supervisor name: عبد الواحد باقر عبد الرضا الشيباني | سند باقر الاعرجي
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:

التحري عن عوامل النمو و مؤشرات الدنا لصفة تحمل الجفاف في بعض التراكيب الوراثية للرز (Oryza sativa L.) == اInvestigation of Growth Factors and DNA Markers for Drought Tolerance in Some Rice (Oryza sativa L.) Genotypes

Author name: اسماء كاطع عريبي
Supervisor name: كاظم محمد ابراهيم | شذى عايد يوسف
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: English
University location: Baghdad
First pages:

دراسة تاثير العوامل الحيوية واللاحيوية في تحفيز الزيوت الاساسية وحامض الروزمارينك في نبات اكليل الجبل Rosmarinus officinalis .L خارج الجسم الحي == Study the Effect of some Biotic and Abiotic Factors on Enhancement of Essential Oils and Rosmarinic Acid in Rosemary Rosmarinus offcinalis L. in vitro

Author name: خليل ابراهيم رشيد المفرجي
Supervisor name: كاظم محمد ابراهيم | صبحي جواد حمزة
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: English
University location: Baghdad
First pages:

عزل وتشخيص مناعي نمطي للخلايا الجذعية المزنشيمية من الحبل السري البشري == Isolation and Immunophentypic Characterization of Mesenchymal Stem Cells from Human Umbilical Cord

Author name: سالي مازن اكرم
Supervisor name: زهراء كامل زيدان
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:

عزل وتشخيص عاثي المكورات العنقودية الذهبية وتقييم نشاطه المضاد للبكتيريا == Isolation and Characterization of Staphylococcus aureus Bacteriophage and Evaluation of Its Antibacterial Activity

Author name: عارف عباس غايب
Supervisor name: ابراهيم عبد الله احمد | حامد خلف احمد
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Master
Language: English
University location: Baghdad
Key words:
  • phage Rih21
  • phage genome analysis
  • bacteriophage
  • S. aureus
  • Staphylococcus aureus phage Rih21
  • staphylococcus aureus
  • bacteriophage
  • novel bacteriophage
  • Staphylococcus phage Rih21
  • MRSA
First pages:
Abstract: The antimicrobial therapy, in the long term, is expected to become ineffective against Staphylococcus aureus (S. aureus) due to its ability to produce new and more virulent strains. Bacteriophages are known to fight bacteria for a very long time and have the ability to adapt to the developing bacteria. In this study, 129 clinical specimens were collected from different places in Baghdad and from different clinical sources during the period from October 13, 2020, to January 26, 2021. Only 45 isolates showed cultural (mannitol salt and blood agar) and biochemical (catalase, coagulase and oxidase test) characteristics that were specific to S. aureus. The identity of S. aureus isolates was further confirmed by using specific primers for amplification and sequencing of the S. aureus thermonuclease (nuc) gene, and the blast result of sequences in the NCBI database was 99% to 100% identical to S. aureus species. Also, the S. aureus isolates were tested for sensitivity of different types of antibiotics, and results revealed that: all S. aureus isolates demonstrated high levels (100%) of resistance against the cefoxitin, penicillin, and ceftazidime. It also showed intermediate levels of resistance against tetracycline 55.56% (25/45 isolates), and azithromycin 46.67% (21/45 isolates), and the lowest levels of resistance was against the erythromycin 44.44% (20/45 isolates), rifampin 17.78% (8/45 isolates), trimethoprim/sulfamethoxazole 17.78% (8/45 isolates), clindamycin 15.56% (7/45 isolates), gentamicin 13.33% (6/45 isolates) and ciprofloxacin 11.11% (5/45 isolates). also found that no antibiotics tested in this study were able to kill all S. aureus isolates, therefore all isolates tested in this study were classified as Methicillin-resistant Staphylococcus aureus. From the hospital waste-water, a novel bacteriophage was isolated and characterized. The enrichment method was used for isolation of Staphylococcus aureus bacteriophage and six rounds of purification based on the plaque assay were used to purify a single type of bacteriophage against S. aureus clinical isolates. According to characterization properties, this bacteriophage belongs to the Siphoviridae family and can form a halo around the phage plaque. The maximum bacteriophage titer was recorded at 37°C and a pH of 7.2. The bacteriophage titers were decrease at 45°C, 50°C, and 55°C, until completely lost its activity at 60°C. This bacteriophage was completely inactivated at highly acidic (3, 4) and basic (10, 11) pH values and lost part of its activity at pH 5, 6, 8, and 9. This bacteriophage had a 44,789 bp linear double-strand DNA genome, and within the genome sequence, there are 61 genes, all of which are encoded into proteins. Although this bacteriophage does not have any virulence factors or antimicrobial resistance genes and had specific lytic activity against some antimicrobial resistance S. aureus clinical isolates. All the features mentioned above about this bacteriophage could make it a valuable antibacterial choice against antimicrobial-resistant S. aureus clinical isolates.
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تاثير المعززات الحيوية على البروتييز المنتج من البكتريا المعزولة من قرحة القدم لمرضى السكري == EFFECT OF PROBIOTICS ON PROTEASE PRODUCING BACTERIA ISOLATED FROM DIABETIC FOOT ULCER

Author name: فرقان ماجد كاظم
Supervisor name: عبد الواحد باقر الشيباني | اسماء علي حسين
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: English
University location: Baghdad

التباين الوراثي لجين SLC2A9 وعلاقته ببعض المظاهر الكيموحيوية في عينة من المرضى العراقيين المصابين بداء النقرس == Genetic polymorphism in SLC2A9 gene and its association with some biochemical aspects in a sample of Iraqi gout patients

Author name: يسر رضا ممدوح النقشلي
Supervisor name: وليد حميد يوسف | عبد الواحد شمخي جابر
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:

التحري الجزيئي عن العناصر المنتقله المصحابة لمضادات الحياة في بكتيريا Escherichia coli المعزولة من التهابات المجاري البولية وحساسيتها للعاثيات == Molecular Detection of Transposable Elements Associated with Antibiotic Resistance in UTI Isolates of Escherichia coli and Phage Susceptibility

Author name: شهد رعد مزعل
Supervisor name: حسن محمد نايف
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: English
University location: Baghdad
First pages:

الفعالية السمية الخلوية والضد ميكروبية لجزيئات الفضة النانوية المخلقة من مستخلص الميرامية Salvia Officinalis على بعض العزلات المرضية == Cytotoxic and Antibacterial Activity of Silver Nanoparticles Synthesized by Salvia officinalis Extract on Some Pathogenic Bacteria

Author name: سارة مهند ياسين عزيز
Supervisor name: رقية محمد ابراهيم | اسماء علي حسين
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:
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