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التوصيف الوراثي لبعض عوامل ضراوة بكتريا E.coli المعزولة من اخماج المسالك البولية == Genotypic Characterization of someVirulence factors inE.coli isolated from UTI

Author name: عميدة علي عطية العبيدي
Supervisor name: وعد محمود رؤوف | كركز محمدثلج
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:

دراسة بعض الجوانب الوبائية والمناعية للخمج بداء المقوسات في مدينة الفلوجة - محافظة الانبار == Study of some epidemiological and immunological aspects of toxoplasmosis in Fallujah city - Al - Anbar province

Author name: محمد سليم اسماعيل ثامر العيساوي
Supervisor name: ابراهيم شعبان داوود | عبد الوهاب بديوي حسين
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: شملت الدراسة جمع عينات دم من النساء المجهضات تراوحت اعمارهن بين (16 - 45) سنة المراجعات لمستشفى الفلوجة العام لمعرفة مدى انتشار طفيلي المقوسة الكوندية Toxoplasma gondii في مدينة الفلوجة - محافظة الانبار بطريقتي تلازن اللاتكس Latex agglutination واختبار الخميرة للادمصاص المناعي (الاليزا) Enzyme linked immunosorbent assay, كذلك قياس مستوى الاضداد IgA , IgE , IgM , IgG والمتممين C4 , C3 وبعض المظاهر المناعية للخلايا البلعمية متعددة اشكال النوى العدلة (PMNs) وبعض التاثيرات المناعية في الفئران البيض والقطط . ظهرت النتائج ان نسبة الخمج الكلية وحسب الفئات العمرية قيد الدراسة (16 - 25) , (26 - 35) , (36 - 45) سنة بطريقة تلازن اللاتكس كانت 43.75% , ( 40.90% , 46.15% , 42.11% ) وبطريقة الاليزا 32.50% (31.82% , 35.90 , 26.31% ) , ونسبة الاخماج الكلية الحادة والمزمنة بطريقة ELISA - IgG , ELISA - IgM وحسب الفئات العمرية كانت 10% (13.64% , 12.82% , 0% ) , 22.50% (18.18% , 23.08% , 26.31 ) على التوالي. قياس مستوى الاضداد IgG , IgM النوعية ضد المقوسة الكوندية وIgE الكمي بطريقة اختبار الاليزا (ELISA ) , وقياس مستوى الضد IgA الكمي والمتممين( Complements) C4 , C3 بطريقة اختبار الانتشار المناعي الشعاعي المفرد Single radial immunodiffusion test اظهرت ارتفاع معنوي في معدل مستوى الاضداد IgG , IgM النوعيين ضد المقوسة الكوندية وعدم وجود فروق معنوية لمعدل مستوى الاضداد IgE , IgA الكميين والمتممين C3 , C4 للنساء المخمجات مقارنة بالسيطرة. اظهر التحليل الاحصائي ان للخمج بطفيلي المقوسة الكوندية T. gondii وجود فرق معنوي على زيادة نشاط الخلايا متعددة اشكال النوى العدلة (PMNs) في بلعمة خميرة الكانديداCandida yeast واختزال صبغةNitroblue tetrazolium reduction وتاثيراﱟ منخفضاﱟ لمعدل عيوشية الخلايا (PMNs ) وهجرة طبيعية للخلايا PMNs)) المعزولة من النساء المخمجات ولكل الفئات العمرية مقارنة بالسيطرة. اظهرت النتائج ان حقن اكياس البيض المضعفة بالاشعة فوق البنفسجية Ultraviolet والمكسرة Ultra - Sonication اعطت تفاعلات واضحة عند اجراء اختبار فرط الحساسية المتاخرDelayed Type Hypersensitivity اذ بلغ اعلى معدل سمك القدم (2.32 , 2.79) ملم وقطر المنطقة المتهيجة (7.79 , 8.78) ملم بعد (24) ساعة في الفئران المحقونة على التوالي ، ان تمنيع الفئران بمحلول اكياس البيض المضعفة واعطائها جرعة تحدي تاثير على انخفاض معامل الكبد والطحال مقارنة بالسيطرة ، وان حقن مستضدات الطفيلي ( اكياس البيض المضعفة ) في الفئران ثم اعطائها للقطط اخر وقلل نسبة اكياس البيض المطروحة من قبل القطط وبلغت نسبة الوقاية الكلية 22.29% مقارنة بالسيطرة الموجبة. | The study included collection of blood samples from aborted women, aged between 16 - 54 years attended the general Fallujah Hospital for the prevalence of the infection with Toxoplasma gondii in Fallujah city - AL - Anbar province by two methods : latex agglutination , Enzyme linked immunosorbent assay , measurement of antibodies concentration of IgM, IgG, IgE, IgA, Complements C3, C4, and some immunological features of cells and some immunological effect in white mice and cats. The results showed that the percentage of total infection and according to age groups in the study (16 - 25), (26 - 35), (36 - 45) by LAT were 43.75% , 40.90% , 46.15% 42.11% respectively and with ELISA test 32.50% , 31.82% , 35.90% , 32.50% respectively , the percentage of total acute infection by ELISA - IgM in accordance with age groups were 10% , 13.64% , 12.82% , 0% whereas total , chronic infection by ELISA - IgG were 22.50% , 18.18% , 23.08% , 26.31% respectively. The measurement of antibodies levels of IgG, IgM, IgE by ELISA, IgA and complements C3, C4 by single radial immunodiffusion showed a significant increase in the average antibodies levels of specific IgM, IgG against T. gondii whereas no significant increase in the average antibody level of IgE, IgA and complement C3 , C4 in the infection women in comparison to control. The infection with T. gondii showed significant difference in the activity of Polymorphonuclear neutrophils (PMNs) in the phagocytosis of candida yeast and reduction of Nitroblue tetrazolium, whereas little effect on viability of PMNs and natural migration of neutrophils isolated from infection women of all age groups in comparison to control. The results of the immunological effects of inoculation of Oocysts attenuated by ultra - violet rays or ultra - sonication into white mice showed, the clear reaction in delayed type hypersensitivity test, the average of highest thickness of mice - foot were 2.79, 2.32 mm. and diameter of the inflamed regions were 8.78, 7.79 mm. 24h. after inoculation, the immunization of mice with attenuated oocysts followed by a challenge dose reshowed a decrease in the factor of liver and spleen in comparison to control. Cats fed mice that fedded by which was inoculated by Oocysts attenuated by ultra - violet rays showed a delay and a decrease in the Oocysts discharge in the feces by 22.29% in comparison to control.

تاثير بعض المستخلصات النباتية على مستوى سكر الدم في ذكور الجرذان السليمة والمصابة بداء السكر التجريبي == Effect of some plant extracts on blood sugar level in normal and experimentally diabetic male rats

Author name: احمد كمال محمد الامـري
Supervisor name: صالح محمد رحيم العبيدي | حسين فاضل حسن بيرقدار
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تم في هذه الدراسة اختبار فعالية المستخلصات المائية لكل من درنات نبات السعد Cyperus rotundus وسيقان واوراق نبات الجعدة Teucrium polium وثمار نبات الخروب Prosopis farcta في خفض مستويات الكلوكوز والكوليسترول والكليسيريدات الثلاثية في دم ذكور الجرذان السليمة والمصابة بداء السكر المستحدث بالالوكسان ومقارنتها مع تاثير الانسولين والكلبنكلامايد . وقد تبين ان المستخلصات المائية للسعد والجعدة والخروب ادت الى انخفاض معنوي في مستوى الكلوكوز في الجرذان السليمة والمصابة بداء السكر . وكما تشير النتائج الى ان المستخلص المائي لدرنات نبات السعد قد ادى الى انخفاض معنوي في مستوى الكوليسترول والكليسيريدات الثلاثية في حين ان المستخلص المائي لثمار نبات الخروب قد احدث انخفاض معنوي في مستوى الكليسيريدات الثلاثية فقط بينما لم يؤد المستخلص المائي لسيقان واوراق نبات الجعدة الى اي انخفاض معنوي في مستوى الكوليسترول والكليسيريدات الثلاثية في الجرذان المصابة بداء السكر . ومن ذلك يمكن الاستنتاج بان هذه المستخلصات المائية يمكن استخدامها في معالجة داء السكر بديلا عن الكلبنكلامايد بعد التاكد مستقبلا من عدم وجود اعراض جانبية لها . | In the present study the effect of aqueous extracts of Cyperus rotundus ,Teucrium polium and Prosopis farcta on the levels of blood glucose , cholesterol and triglycerides were investigated in normal and alloxan - induced diabetic rats. The results were compared with those treated with insulin and glibenclamide. The results indicated a significant decrease in glucose level in normal and diabetic rats. The aqueous extract of Cyperus rotundus produced significant decrease in cholesterol and triglyceride levels but the aqueous extract of Prosopis farcta produced significant decrease only in triglyceride levels wherease aqueous extract of Teucrium polium produced no significant decrease in cholesterol and triglyceride levels in diabetic rats. The results obtained in this study suggest that these extracts may be used for the treatment of diabetes mellitus in place of glibenclamide after be sure that there is no side effects for it. However, further studies must be done in this aspects in the future.

دراسة معدل التكسر الازموزي لكريات الدم الحمر وعلاقته بعدد من المكونات الدموية والمتغيرات الكيموحيوية لدى المرضى المصابين ببعض امراض القلب == A Study on the Osmotic Fragility Rate of Red Blood Cells and their Relationship with some Biochemical characteristics of Patients Suffering from some Heart Diseases

Author name: وسن سرحان عبيد الشمري
Supervisor name: صالح محمد رحيم العبيدي | موسى جاسم محمد الحميش
General topic: Biology
Specific topic: Zoology
Degree: Master
University: Tikrit University - College Of Science
Language: Arabic
University location: Salahaddin
First pages:
Abstract: يهدف البحث الى دراسة معدل التكسر الازموزي لكريات الدم الحمر لدى المرضى المصابين ببعض امراض القلب ,والتغيرات الحاصلة في بعض المقاييس الكيموحيوية والدموية ذات العلاقة بامراض القلب ومنها الانزيمات ( Asprtatransaminase , Alanintransaminase , Lacatdehydrogenas , Creatin kinas) والدهون ( Lipid Profile ) والكلوكوز وبعض فحوصات الدم وهي : - Heamoglobin (Hb) الهيموكلوبين, Erythrocyte sedimentation Rate (ESR) معدل ترسيب كريات الدم الحمر, حجم كريات الدم المضغوطة (Packed Cell Volume (PCV . شملت الدراسة 135 مريضا ومريضة من الراقدين في وحدة العناية المركزة في مستشفى تكريت التعليمي ، وتم تقسيمها على اربعة مجاميع : - المجموعة الاولى (A) : - المرضى المصابين بالمتلازمة التاجية الحادة Acute coronary syndrome (ACS) وقد شملت المصابين بالذبحة الصدرية Angina pectoris وعددهم 29 ( 18 ذكور ، 11 اناث ) ومعدل اعمارهم تتراوح ما بين ( 45 - 65 ) سنة والمصابين باحتشاء العضلة القلبية Myocardial infarction ( MI) وعددهم (52) ( 30 ذكور و22 اناث ) واعمارهم تتراوح ما بين ( 45 - 70 ) سنة . المجموعة الثانية (B) : - ضمت المصابين بعجز القلب Heart Failure وعددهم 20 ( 10 ذكور و10 اناث ) واعمارهم تتراوح ما بين ( 45 - 65 ) سنة .المجموعة الثالثة (C) : - شملت المصابين بالارتجاف الاذيني Atrial flutter ( AF) وعددهم 18 ( 10 ذكور و8 اناث ) واعمارهم تتراوح ما بين ( 50 - 70 ) سنة .المجموعة الرابعة (D) : - والاخيرة وتضم المصابين بالوذمة الرئوية Pulmonary edema)) وعددهم 15 ( 4 ذكور و11 اناث ) واعمارهم تتراوح مابين ( 65 - 75 ) سنة .اما مجموعة السيطرة فقد ضمت 25 فردا (10 ذكور 15 اناث ) من الاصحاء والذين تتراوح اعمارهم مابين( 45 - 65 ) سنة. واظهرت الدراسة الحالية ما يلي : - 1 - انخفاض معدل التكسر الازموزي Osmotic fragilityمعنويا وعند مستوى (p<0.01) لدى المرضى المصابين ببعض امراض القلب مقارنة بمجموعة السيطرة عدا المصابين بالوذمة الرئوية ، كما سجلت مجموعة المصابين بالارتجاف الاذيني انخفاضا معنويا مقارنة بالمجاميع المرضية الاخرى ومجموعة السيطرة ولدى كلا الجنسين.2 - ارتفاع تركيز الكولسترول الكلي معنويا عند مستوى(p<0.01) لدى المجاميع المرضية مقارنة بمجموعة السيطرة ولكلا الجنسين كما ارتفع مستوى الكولسترول لدى المصابين بالمتلازمة التاجية الحادة , وعجز القلب . اما عند المقارنة ما بين الذكور والاناث هنالك ارتفاعا معنويا لدى الاناث المصابات بالوذمة الرئوية مقارنة بالذكورP < 0.01 3 - ارتفاع تركيز البروتينات الدهنية واطئة الكثافة LDL - chol معنويا لدى المجاميع المرضية مقارنة بمجموعة السيطرة وبمستوى معنوية P < 0.01 )) عدا الذكور المصابين بالارتجاف الاذيني حيث كانت النسب ضمن الحدود الطبيعية ولا يوجد فرقا معنويا بين الذكور والاناث .4 - انخفاض تركيز البروتينات الدهنية عالية الكثافة HDL - cholمعنويا وبمستوى P < 0.01) ) لدى مجاميع المرضى مقارنة بمجموعة السيطرة (P < 0.01) ولدى المقارنة ما بين الذكور والاناث نجد ان هنالك ارتفاع معنوي للاناث المصابات بعجز القلب مقارنة بالذكور .5 - ارتفاع معنوي في تركيز الكليسيريدات الثلاثية عند مستوى(P< 0.01) في المجاميع المرضية ( الذكور والاناث ) مقارنة بمجموعة السيطرة ولا توجد فروقا معنوية بين الاناث والذكور في المجاميع كافة .6 - ارتفاع تركيز البروتينات الدهنية واطئة الكثافة جدا VLDL - cholلدى المجاميع المرضية ( الذكور والاناث ) وبمستوى معنوية P < 0.01) ) مقارنة بمجموعة السيطرة ولا توجد فروق معنوية بين الذكور والاناث في المجاميع كافة .7 - ارتفاع في تركيز الكلوكوز لدى المجاميع المرضية ( الذكور والاناث ) وعند مستوى معنوية P < 0.01) ) مقارنة بمجموعة السيطرة في حين لا توجد فروقا معنوية بين الذكور والاناث في المجاميع كافة .8 - عدم وجود فروقا معنوية بين المرضى ومجموعة السيطرة في تركيز PCV%, Hb )) حيث كانت ضمن الحدود الطبيعية في حين كانت مستويات ESR مرتفعة معنويا وبمستوى (P< 0.01) لدى المرضى مقارنة بمجموعة السيطرة .9 - ارتفاع فعالية الانزيمات AST, LDH , CPK معنويا عند مستوى (P< 0.01) لدى المجاميع المرضية مقارنة بمجموعة السيطرة, في حين لا توجد فروقا معنوية بين المرضى ومجموعة السيطرة بالنسبة للانزيم ALT . | This study was carried out at the Tikrit Hospital , the Central Care the study sample included , 135 patients of both sexes . The patients were assigned to four groups . The first group represented the patients of the cute coronary Syndrome (ACS), who were suffering from both the angina pectoris (AP) 29 patients ( 18 male and 11 female ) , their age ranged from 45 - 65 years, and the Myocardial Infraction ( MI ) ( 52 patients , 30 male and 22 female ), their age ranged between 45 - 70 years . The second group was related to the patients with Heart failure (HF) ( 20 patients , 10 male and 10 female ) , their age ranged from 45 - 65 years . The third group was for the patients suffering from Artial Flutter (AF) , ( 8 patients , 10 male and 18 female ) . The fourth group was dealt with the patients suffering from pulmonary Edema (PE) (15 patients 4 male and 11 female , their age ranged from , 65 - 75 years . The results obtained can be summarized as follows : 1 - A decrease in the Osmotic fragility rate was recorded for all the patients groups , with the exception of the (PE) disease group , when compared with the Control group . However the (AF) disease patients showed a significant decrease ( P < 0.05 ) , as compared with other disease patients group and the control for both sexes . 2 - The total Cholestrol level was found to be higher in the patients groups for both sexes . The highest cholesterol level was found in patients suffering from both the ACS and the H F diseases . The females suffering from PE disease showed a higher total cholesterol level than the males ( P < 0.01 ) . 3 - An increase in the LDH - cholesterol level was observed for all patients groups when compared with the control group with the exception of males suffering from (AF) disease , who showed a normal range percentages . Besides the difference between sexes was not significant . 4 - HDL - cholesterol level for patients of all groups was found to be lower than that of the control group ( P <0.01) moreover a significant increase for females suffering from HF disease ( P < 0.01 ) 5 - The triglyceride concenterat9ion was recorded to be higher for the patient groups ( males and females ) , when compared with the control group ( P < 0.0 ) . Never the less the differences between sexes were not significant . 6 - The VLDL - cholesterol concentration for the patients of all disease groups was recorded to be higher , when compared with the control group ( P<0.01). Besides the difference between sexes was not significant for this trait . 7 - An increase in the glucose concentration level for the patients of all disease groups , as compared with the control group was revealed ( P <0.01) . The difference between sexes was not significaul . 8 - Anon - significant different between the patients of all the disease groups and the control group , regarding Hemoglobin Hb and the packed cell volume PCV, percentages were observed ( P < 0.01 ), Nevertheless the values for ( Erythrocyte sedimentation Rate) (ESR) were found to be higher for the patients of the disease groups when compared with control group . 9 - Enzyme Concentrations of (CPK , LDH ,AST) were found to be higher in the patients of all the disease groups , when compared with the control groups ( P <0.01) .10 - The differences between patients of all the disease groups and the control group regarding ( ALT ) enzyme were found

تقييم اختبار البروكالســيتونين في التشخيص المبكر لتسمم الدم الجرثومي في الاطفال حديثي الولادة في مستشفى تــكريــت التعليــمي == Evaluation of Procalcitonin Test for Early Diagnosis of Neonatal Sepsis in Tikrit - Teaching Hospital

Author name: سـهى ماهــر عبـد الرشـــــيد
Supervisor name: وعد محمود رؤوف | موسى جاسم محمد
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Tikrit University - College Of Science
Language: English
University location: Salahaddin
First pages:
Abstract: ان تشخيص الخمج ضمن فئة حديثي الولادة يعتبر بحد ذاته تحد , نظرا لكون الاختبارات التقليدية غالبا ما تؤدي الى التضليل بدل التشخيص . صممت هذه الدراسة لاجل البت بامكانية استخدام اختبار مستوى البروكالسيتونين كمعلم للتشخيص المبكر للحالات المشكوك باصابتها بتسمم الدم الجرثومي في حديثي الولادة بالمقارنه مع زرع الدم, C - reactive protein , عدم خلايا الدم البيض الكلي والتفريقي. تم جمع العينات التي اشتملت على كلا الجنسين خلال الفترة الزمنيه من شهر اب سنة 2008 ولغاية شهر اذار سنة 2009 من حديثي الولادة الوافدين الى ردهات الخدج والاطفال في مستشفى تكريت التعليمي والمشخصه سريرا من قبل طبيب الاطفال المختص على اشتباه اصابتهم بالمرض. لقد اشتملت الدراسه على 114 حاله اجري فحص زرع الدم في جميعها بينما اجريت فحوصات الدم ل 102 حاله , تم فحص CRP ل 84 حالة واختبر مستوى البروكالسيتونين في 73 حالة حيث يعزى عدم تساوي العدد الى عدم كفاية الدم لاجراء جميع الاختبارات . اظهرت نتائج زرع الدم ان 53 (46%) من العينات كانت موجبه منها, 32 (60%) ذكور و21 (40%) اناث . عدد حالات زرع الدم السالبة كانت 61 ( 54%) منها , 31 (51%) اناث و30 (49%) ذكور.اظهرت نتيجة التشخيص المختبري بان العزلات البكتيرية الاكثر شيوعيا كانت من جنس البكتريا العنقوديه السالبة لانزيم التجلط ومن Nocardia spp. لانزيم التخثر بنسبة 22% لكليهما تتبعها بكتريا Listeria monocytogenes بنسبة 17% تليها Klebsiella pneumoniae بنسبة 9% و6 % لكل منPseudomonas aeruginosa وStaphylococcus aureus اما باقي الانواع (Citrobacter diversus, Serratia marscesens, Serratia liquifascens, Lactobacillus spp., Peptostreptococcus spp., Micrococcus spp., Streptococcus pyogenes ) فقد كانت غير شائعة واعطت نسب 2% لكل واحد . بينت هذه الدراسة ان حملة تسمم الدم الجرثومي المتاخر سجلت 67% من الحالات بينما الباقي من النسبه ( 33%) كان ل حملة تسمم الدم الجرثومي المبكر .لقد كان عدد حالات التسمم الجرثومي المؤكد بين الذكور اعلى 32 (60 ) منه في الاناث 21 (40%). تبين ان من بين جميع المظاهر السريريه , قلة الرضاعة شكل اعلى نسبة بينما اعراض تبقع الجلد والقباع اعطت اقل نسبة, كما وبينت بان 30% فقط من الاصابات كانت عند الاطفال ذوي الوزن الطبيعي بينما شكل الاطفال الذين يقل وزنهم عن الطبيعي نسبة 70% . اظهرت دراسة عوامل الخطورة في الامهات بان العامل الاكثر شيوعا كان تمزق الغشاء الامنيوني لفترة تزيد عن 18 ساعة يتبعه عامل التهاب المجاري البولية والحمى واختناق داخل الرحم بالتتابع. نسبة الوفيات كانت اعلى في الحمله المتاخره لتسمم الدم كما كانت نسبة الوفيات اعلى عند حديثي الولادة ناقصي الوزن عنه عند ذوي الوزن الطبيعي احصائيا, كان لذوي الوزن الاقل من الطبيعي اهميه من الناحيه الاحصائيه . اظهر اختبار فحص الحساسية ان عزلات البكتريا السالبة لصبغة كرام كانت ذات مقاومه عاليه للمضادات المستخدمة في الدراسة الحالية بينما كل عزلة من عزلات البكتريا الموجبة لصبغة كرام كان لها نمط مقاومه مختلف للمضادات . كما واظهرت الدراسة لعوامل الضراوة بان العزلات البكترية السالبة لصبغة كرام كانت موجبة لاختبار انتاج انزيم اليوريز واختبار انزيم الهيمولايسن واختبار انزيم اللايبيز واللسيثينيز والبروتييزبنسبة 85% , 100% , 100% , 100% و62% على التوالي بينما كانت العزلات الموجبة لصبغة كرام موجبة للاختبارات بنسبة 61% , 100%, 89%, 94% و785 عالتوالي. اظهرت فحوصات الدم الروتينيه والتي كانت ذات قيمه معنويه بان 33% من بين حالات زرع الدم الموجبة كان عدد خلايا الدم البيض فيها منخفض , 19% من الحالات كان عدد الخلايا البيض مرتفع اما باقي النسبة المؤية فقد كان نتيجة فحص عد خلايا ضمن المستوى الطبيعي فيما يتعلق بحالة تواجد الخلايا العدله غير الناضجة في الدم المحيطي فقد بينت الدراسه ان 79% من حالات زرع الدم الموجبة كانت ايضا موجبة من ناحية تواجد الخلايا العدله غير الناضجه فالدم بينما باقي النسبة ضمن حالات زرع الدم الموجب فقد كانت سالبة لتواجد هذا النوع من الخلايا.علما ان الاختلافات الاحصائيه المتعلقه بالخلايا العدله غير الناضجه كانت ذات قيمة معنويه عاليه. بالنسبة لفحص CRP كانت الفروقات الاحصائيه غير ذات قيمه معنويه مابين الحالات الموجبه لزرع الدم عن الحالات السالبه . وفيما يخص نتيجة فحص PCT الذي تم قياسه باستخدام طريقة القياس شبة الكمي الكرماتوغرافيه المناعيه فقط ظهر ان تركيز البروكالسيتونين في المصل لم يكن ذا فرق احصائي معنوي بين المرضى المصابين ببكتريا موجبه لصبغة كرام عن المصابين ببكتريا سالبة لصبغة كرام. اظهرت نتائج دراستنا ان 21% من حالات زرع الدم الموجبه اعطت مستوى بروكالسيتونين اقل من 0.5 نانوغرام لكل مل وباقي النسبة توزعت بين مستويات البروكالسيتونين الباقيه ≥0.5 ≥2 ≥10. بالنسبة لحالات زرع الدم السالبة فقد اظهرت نتيجة قياس مستوى PCT بان نسبة 49% كان ضمن مستوى البروكالسيتونين<0.5 نانوغرام لكل مل بينما 51% كانت ضمن المستويات ≥0.5 ≥2 ≥ 10 نانوغرام لكل مل بمعنى لايمكن استبعاد حصول اصابة جهازيه حتى لو كانت نتيجة الزرع الدم سلبية. كما لم تتوافق نتيجة البروكالسيتونين مع نتيجة فحص الCRP لكل الحالات فقد سجلت اعلى مستويات للبروكالسيتونين بينما كان فحص ال CRP لها سالب , ومن مقارنة نتائج عد الخلايا البيض مع نتيجة البروكالسيتونين التي اجريت لنفس الحالات ظهر بان 68 % من الحالات كانت ضمن معدل عدد الخلايا الطبيعي بالوقت الذي اعطت مستوى عالي للبروكالسيتنوين≤10 نانوغرام لكل مل و18% فقط من الحالات كان عدد الخلايا البيض مرتفع وباقي النسبة اشتملت عل حالات انخفض بها عدد الخلايا البيض عن الطبيعي . من بين كل الحالات الي اجري لها فحص مستوى البروكالسيتونين سجلت 39 حالة بنسبة 53 % ظهرت بها الخلايا البيض العدلة غير الناضجة فالدم المحيطي . عند مقارنة اختبار البروكالسيتونين مع باقي الفحوصات الروتينه المستخدمه بالدراسه اتضح بانه اكثر حساسية في تشخيص تسمم الدم الجرثومي وفقا لذلك , نستنتج بان البروكالسيتونين هو محدد اكثر حساسية من الفحوصات التقليدية( فحص زرع الدم وفحص ال CRP وفحص عد الخلايا البيض) في التشخيص المبكر لتجرثم الدم الناجم عن البكتريا عند الاطفال حديثي الولاده . | The diagnosis of neonatal infection is challenging because traditional markers of infection are often misleading instead of diagnosing. This study was conducted to determine Procalcitonin (PCT) level as an early marker for diagnosis of suspected sepsis in neonates in comparison with C - reactive protein, blood culture, total and differential white blood cell counts. From August, 2008 till March, 2009, samples were taken from neonates (males and females) admitted to the pediatrics - neonatal wards at Tikrit Teaching Hospital who were clinically suspected with sepsis as diagnosed by the physician in the hospital with any features suggestive of sepsis. The study includes a total of 114 cases. Blood cultures were done for all cases. Hematological tests were performed for 102 cases. C - reactive protein (CRP) was determined in 84 cases, while Procalcitonin level was determined in 73 cases. The differences in the number of samples involved in each test were due to the inadequate amount of blood specimens to perform all investigations. The results of blood culture showed that 53 (46%) of samples were positive, of them 32 (60%) were males and 21 (40%) were females. The number of negative cases were 61 (54%), of them 31 (51%) were females and 30 (49%)were males. The results of identification showed that the most frequent bacterial isolates were Coagulase Negative Staphylococci and Nocardia spp. (22% for each), followed by Listeria monocytogenes 17%, Klebsiella pneumoniae, 9% Pseudomonas aeruginosa 6% and Staphylococcus aureus 6%, while (Citrobacter diversus, Serratia marscesens, Serratia liquifascens, Lactobacillus spp., Peptostreptococcus spp., Micrococcus spp., Streptococcus pyogenes) were frequently less isolated recording 2% for each . This study revealed that, late - sepsis was recorded in 67% of cases while the rest (33%) were with early - sepsis and also revealed that. The number of proven sepsis were higher in males 32 (60%) than in females 21 (40%). Among all clinical manifestation, poor feeding had the highest proportion, while mottling and grunting had the smallest proportion. Only 30% of neonates were of normal birth weight, while 70% were low birth weight, very low birth weight and, extremely low birth weight respectively. Study of maternal risk factors revealed that the most frequent factor was the Prolonged Rupture of membrane followed by maternally UTI, fever and intrapartum asphyxia, respectively. The fatality rate was higher in late - sepsis cases, also it was higher in low birth weight than in normal birth weight neonates, statistically VLBW had the highest value. The antibiotics susceptibility test revealed that Gram negative isolates showed high resistance to antibiotics used in the present study. Each isolate of the Gram positive bacteria showed different pattern of resistance against the antibiotics used. Results of virulence factor showed that 85%, 100%, 100% , 100% and 62% of Gram negative isolates and 61%, 100% , 89%, 94% and 78% of Gram positive isolated produced Urease , Hemolysin, Lipase, Lecithinase and alkaline protease, respectively. Results of routine hematological tests at which the differences were statistically significant showed that 33% of culture positive cases had leucopenia, 19% of cases had leukocytosis, while the rest were in the category of normal range. Concerning the shift to left, results showed that (79%) of culture positive cases were recorded as positive and the rest were negative shift to left neutrophil the differences concerning with the shift left were statistically highly significant. For CRP, the differences were not significant among culture positive and culture neative cases. With reference to PCT test which had been measured using the immunochromatographic semi - quantitative method, that serum procalcitonin concentrations were not significantly different between the patients with Gram - positive and those with Gram negative infections. Results of our study showed that 21% of culture positive cases were <0.5 ng/ml (which were regarded as negative), while the rest which were with high level of PCT were distributed among levels of PCT≥0.5 ≥2 ≥10 ng/ml. As for culture negative cases, 49% of PCT were <0.5 ng/ml, while 51% of cases were in levels of ≥0.5 ≥2 ≥10 ng/ml which means that systemic infections cannot be excluded though they were culture negative. In addition PCT results did not fit with CRP all the time since some cases recorded higher level of PCT ≥10 ng/ml despite they were negative for CRP. The comparison between results of PCT and WBC for the same performed cases showed that 68% of cases were in the category of normal WBC range, meanwhile they had high level of PCT. Only 18% were in the category of leukocytosis, and the rest of percentage were in the category of leucopenia. Among all cases performed for PCT, 39 (53%) cases had left shift neutrophil in the peripheral blood when the differential counts were performed. when PCT was compared with other routinely measured parameters used in this study, It seemed to be significantly more sensitive in sepsis diagnosis. Accordingly, we can conclude that PCT is a more sensitive marker than other traditional tests (blood culture, CRP and WBC count) that could be used in early diagnosis of bacterial sepsis in neonates.

تقدير مستويات هرمونات الغدة الدرقية وبعض الهرمونات ذات العلاقة في الحمل الطبيعي والحمل المهدد بالاجهاض في مدينة تكريت == Estimation of Thyroid hormones concentrations and some related hormones in normal pregnancy and threatened abortion in Tikrit

Author name: علياء صالح جواد الناصري
Supervisor name: زيد محمد مبارك المهداوي | هدية احمد خليل
General topic: Biology
Specific topic: Zoology
Degree: Master
University: Tikrit University - College Of Science
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تهدف هذه الدراسة الى اظهار العلاقة ما بين مستويات هرمونات الغدة الدرقية Thyroid hormones (الثايرونين ثلاثي اليود ,T3 الثايروكسين T4) وبين حدوث حالات الحمل المهدد بالاجهاض ، بالاضافة الى تناول دراسة التغيرات الحاصلة في هرموني الاستروجين E2 والبرولاكتين PRL وكذلك الهرمون المحفز للدرقية TSH وعدد من المقاييس الدموية والكيموحيوية (تركيز هيموغلوبين الدم Hb , النسبة المئوية لحجم خلايا الدم المضغوطة PCV% , تركيز البروتين الكلي Total protein ) اثناء الحمل الطبيعي والحمل المهدد بالاجهاض . شملت الدراسة 280 عينة دم تم اخذها من النساء اللاتي تتراوح اعمارهن بين 15 - 36 سنة وممن راجعن مستشفى تكريت التعليمي والعيادات الخارجية لبعض الاطباء, وتم تقسيمها الى ثلاثة مجاميع وهي : الاولى ضمت 160 عينة دم من النساء ذوات الحمل الطبيعي ، والثانية ضمت 80 عينة دم من النساء ذوات الحمل المهدد بالاجهاض والثالثة ضمت 40 عينة دم من النساء غير الحوامل كمجموعة سيطرة . اظهرت نتائج هذه الدراسة ارتفاعا معنويا في تركيز هرمونات الدرقية T3 وT4 في مجموعة النساء ذوات الحمل الطبيعي ( ولجميع اشهر الحمل ) عند مستوى معنوية ( p < 0.01 ) مقارنة مع مجموعة السيطرة ، وكذلك حصول انخفاض معنوي في تركيز هرمونات الدرقية T3 وT4 في مجموعة النساء ذوات الحمل المهدد بالاجهاض (وللاشهر الاربعة الاولى من الحمل ) عند مستوى معنوية ( p < 0.01 ) مقارنة مع مجموعة السيطرة ، بينما لم تسجل النتائج وجود فروق معنوية بين مجموعة النساء ذوات الحمل الطبيعي ( ولجميع اشهر الحمل ) ومجموعة السيطرة في تركيز هرمون TSH,اذ كانت ضمن الحدود الطبيعية , في حين تم تسجيل ارتفاع معنوي في تركيز هرمون TSH في مجموعة النساء ذوات الحمل المهدد بالاجهاض ( للاشهر الاربعة الاولى من الحمل ) عند مستوى معنوية ( p < 0.01 ) مقارنة مع مجموعة السيطرة . اظهرت النتائج ارتفاعا معنويا في تركيز هرمون الاستروجين E2 في مجموعة النساء ذوات الحمل الطبيعي ( ولجميع اشهر الحمل ) مقارنة مع مجموعة السيطرة وعند مستوى معنوية ( p < 0.01 ) وعلى خلاف ذلك اظهر تركيز هرمون الاستروجين E2 انخفاضا معنويا في مجموعة النساء ذوات الحمل المهدد بالاجهاض ( للاشهر الاربعة الاولى من الحمل ) مقارنة مع مجموعة السيطرة وعلى مستوى معنوية ( p < 0.01 ) . وسجلت النتائج ارتفاعا معنويا في تركيز هرمون البرولاكتين PRL في مجموعتي النساء ذوات الحمل الطبيعي والنساء ذوات الحمل المهدد بالاجهاض ( ولجميع اشهر الحمل) عند مستوى معنوية ( p < 0.01 ) مقارنة مع مجموعة السيطرة. ولوحظ ان هناك انخفاضا معنويا في تركيز البروتين الكلي في مجموعتي النساء ذوات الحمل الطبيعي والنساء ذوات الحمل المهدد بالاجهاض ولجميع اشهر الحمل مقارنة مع مجموعة السيطرة وعند مستوى معنوية ( p < 0.01 ) .واخيرا اظهرت النتائج انخفاضا معنويا في قيم بعض مقاييس الدم ( % PCV , Hb ) وفي كلا المجموعتين المدروستين ولجميع اشهر الحمل مقارنة مع مجموعة السيطرة وعند مستوى معنوية ( p < 0.01 ) . وقد اوضحت الدراسة وجود علاقة بين الانخفاض في مستوى بعض هرمونات الدرقية ونسبة معينة من النساء الحوامل المهدد حملهن بالاجهاض مما يعطي تفسيرا بان لهذه الغدة دورا مهما في التاثير على طبيعة الحمل والحفاظ عليه . | This study aims to show the relationship between thyroid gland hormones levels (Triiodothyronine T3, Thyroxine T4 ) and the incidence of threatened abortion pregnancy . the changes in Estrogen , Prolactin and Thyroid stimulating hormone were also investigated. The characteristics included in this work were some blood measurements and biochemical traits ( Hemoglobin Hb , Packed cell volume PCV% and the total protein concentration ) . The work performed through using 280 blood samples taken from women ( 15 - 36 years age ) , attending Tikrit Teaching Hospital and some private medical clinc s. The sample was divided into three groups , the first group represented normal pregnant women ( 160 blood samples ) and the second group included the threatened abortion pregnancy women ( 80 blood samples ) , where as the third group represented a non pregnant women ( 40 blood samples ) which considered as a control group . The results obtained revealed a significant increase ( p < 0.01 ) in the concentration of thyroid hormones ( T3, T4 ) for the normal pregnant women , for all the pregnant months , when compared with the control group . A significant decrease ( p < 0.01 ) was also detected in the abortive threatened pregnancy women , when compared with control group women , regarding T3 and T4 hormones concentration for the first four months of pregnancy . Non significant difference between the normal pregnant women group and the control group. It was evident that TSH concentration increase significantly ( p < 0.01 ) in the threatened abortion pregnant women for the first four pregnancy months when compared with the control group women . Estrogen hormone concentration was found to be significantly ( p < 0.01 ) higher in the normal pregnant women for all pregnancy months as compared with the control group women . On the contrary a significant decrease in estrogen hormone concentral in the abortive threatened women for the first four pregnancy months when compared the control group was found .The prolactin hormone concentration was found to be higher significantly ( p < 0.01 ) in both the normal pregnant women and the abortive threatened pregnant women for all the pregnancy months when compared with the control group . It was revealed that the total protein concentration level for both the normal and the abortive threatened pregnant women decreased significantly ( p < 0.01 ) for all pregnancy months . A significant decrease ( p < 0.01 ) in some blood characteristics ( Hb , PCV % ) for both the normal and abortive threatened pregnant women for all pregnancy months were recorded as compared with the control group . This study confirmed an apparent relationship between the decrease of some thyroid gland hormones concentration and the existence of some percentages of abortive threatened pregnant women , which indicate the importance of thyroid gland effect on the nature and the persistence of pregnancy .

دراسة بعض الملوثات الصناعية وتاثيرها في الصفات التشريحية لبعض الانواع النباتية في قضاء سامراء == Study of Some Industrial Pollutants And Their Impact In The Anatomical Characteristics of Some Plant Species In Samarra

Author name: احمد يونس حمادي الكنعاني
Supervisor name: طالب عويد الخزرجي | ياسين محمد احمد الدليمي
General topic: Biology
Specific topic: Plant - Diseases
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: ان نمو النباتات في مواقع ملوثة قد يعرض صحة المستهلك للخطر سواء اكان انسان? او حيوان?، لذلك انجز هذا البحث لتقييم التلوث بالعناصر الثقيلة في مياه المخلفات الصناعية للحي الصناعي في مدينة سامراء وفي التربة والنباتات الواقعة على مجرى هذه المياه الجارية في ساق | The growth of plants in contaminated sites may expose consumers' health at risk, whether a human being or animal so accomplished this research to assess the pollution heavy elements in the waters of industrial waste of the neighborhood's industrial city of Samarra, in soil and plants located on the course of this running water in a stream length of 800 m even downstream Tigris River, as they are put waste industrial district as well as sewage water into the river is directly without treatment, as the study was to take part of the water and soil of that region as well as four types of plants that grow where is the tail of the seven, mint, sugar cane and corporal parents, study Some qualities Physical and chemical soil and water in addition to study some changes anatomical got to plants due to pollution, and that by dividing the study area into four zones as is the region (1) part near the industrial district and region (2) is located just 200 meters from the site of No. (1) towards the river, followed by site number (3), which is 200 m from the site of No. (2) The region (4) is located near the bank of the river and about 200 m from the site number (3) and samples were taken control of the West Bank of the Tigris River, was measurement of both pH and electrical conductivity and salinity in addition to measuring some heavy elements (iron, copper, cadmium) of soil and water, as well as the measured requirement vital for oxygen and the degree of turbidity of the water, also included the study to measure some heavy elements (iron, copper, cadmium) in plant and study the anatomical changes that occur due to the pollution produced by the launch of industrial waste. The results of the study that there is variation among study sites for each of the regions of the east bank of the river (the contaminated samples) and the West Bank of the river (the control) for most attributes Physical and chemical water and soil as well as for changes anatomical plants between contaminated samples and the control and in general has The results are within the limits permitted by the World Health Organization, the results indicate the high concentration of copper in the soil compared with iron and cadmium as the highest concentration has 10 ppm at the site (1), while the concentration of sample control 0.17 ppm The iron was higher focus him in the soil is 5.2 ppm in both locations (3) and (4), while the concentration of sample control 3.3 ppm The cadmium was the highest concentration has 0.43 ppm at the site (2), while the concentration of sample control 0.15 ppm and these concentrations are all not exceeding the allowable limit for the World Health Organization (for cadmium 1.5ppm of iron 120ppm copper 40ppm) respectively, and the results showed variation in pH between the soil and water for study sites on the one hand and sample control on the other hand, as shown soil samples to pH moderate among the four sites studied, where ranged pH between 7.26 - 6.33 The sample control was pH 8.64 As for water, the results showed basal water sites studied ranged pH between 6.8 to 5.8 which is comparable to Las pH for sample control 8.5, As for connectivity electrical has emerged variation between samples studied and sample control for soil and water and showed samples of soil and water at the site (2) the extent of the variation connectivity electrical them at the sites (3, 2, 1) and which are all higher than in the sample control, with varied degrees connectivity for soil samples between 14, 200 - 2860 Maekerosemins / cm The sample control 2300 Maekerosemins / cm The degree connectivity for water samples 2810 - 2450 Maekerosemins / cm The sample control of water was connectivity 1457 Maekerosemins / cm, for salinity, the results showed an increase in salinity of soil samples and water compared to a sample control ranged salinity in the soil between 8.92 - 1.79 g / l, while a control sample was salinity 1.438 g / l The salinity in the water was between 1.759 - 1.532 g / l The control sample was 0.907 g / l The results showed that the sample at the site (2) the degree of salinity have is higher than the rest of the other samples in soil and water. Results showed high copper for iron in the water was the highest percentage of copper in the signatories (4), (3) 0.05ppm The sample control has, (2.1) compared to a control sample, where he was between 55 - 20 mg / L, while a control sample 3.8 mg / liter, while for water Ekorh has ranged between four samples where Ekorh was water between 12.36 - 2.97 naphthalene The control sample was Ekorh water 2.1 Naphthalene. The results showed that the concentration of iron was high in most plants for copper and cadmium, as was the highest concentration of iron in the plant reeds 24 ppm as was assembled in the root zone of the plant which is within the limits permitted by the World Health Organization while the highest concentration of iron in the area of control of the plant reed it reached 17.5 ppm as it was assembled in the root also Copper was the highest concentration in the plant sergeant cock 0.89 ppm as it was assembled in the root while the focus was in control very few samples The cadmium is 0.1 ppm in all plants is within the allowable limits by the World Health Organization, while in the side anatomical has emerged is a difference between the samples studied and samples of control, as happened increase in the dimensions of the vascular bundles of the leg, as well as an increase in the thickness of the crust in the samples studied compared a sample of control as well as an increase in the thickness of the tissue foundation and a decrease in the number of packets Vascular plant roots as well as increase the thickness of the securities, an increase of pollution in the studied plants compared to a control sample..

دراسة عدد من المتغيرات الفسلجية والمناعية لدى النساء المصابات بالتهاب السبيل البولي في مدينة تكريت == Study Some of Physiological And Immunological Parameters In Women With Urinary Tract Infections In Tikrit City

Author name: لبنى عبد الجبار ياسين
Supervisor name: موسى جاسم محمد الحميش
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: ان الهدف الرئيس لهذه الدراسة هو معرفة علاقة الانترلوكينات(IL - 2, IL - 4, IL - 6) ببعض الهرمونات لدى النساء المصابات بالتهاب السبيل البولي.تضمنت هذه الدراسة 116عينة دم من النساء المراجعات للعيادات الخارجية التابعة لمستشفى تكريت التعليمي اللواتي تترواحت اع | The main objective of this study is to investigate the relationship of Interleukines (IL - 2, IL - 4, and IL - 6) with hormones in women with inflammation of the urinary tract. This study involved (116) women visiting outpatient clinic of Tikrit Teaching Hospital for the period from September 2011 until February 2012. The ages of women were 16 - 50 years, and blood samples were taken for each of these women for the purpose of determining the levels of sex hormones (FSH, LH, estrogen, progesterone, testosterone) and the levels Interleukines (IL - 6, IL - 4, IL - 2) and identify some of the variables blood parameters (WBC, ESR) and C - reactive protein Twenty samples were taken from the blood of women who are not infected and non - pregnant as a control group samples. The patients divided into three groups, which included non - pregnant women infected and pregnant women who are infected and pregnant non - infected urinary tract (UTI) also samples of the study was divided by periods of gestation into three periods included the first period (1 - 12 weeks) and the second period (12 - 24 weeks) and the third period (24 - 40 weeks) of pregnancy The results of the current study showed that there is significant increase in ESR for a pregnant women infected and pregnant women non - effected with UTI when compared to control (P ? 0.01) and highest level of ESR was in the last third of pregnancy, The total Count of white blood cells (WBC), results showed a significant difference between the three groups compared to the control group, had the highest level for (WBC) in the last third of pregnancy. As for the study of hormones when measuring the level of the hormone LH showed a significant decrease (P ? 0.05) for the three groups compared to control and the steepest decline of hormone level was in the last third of pregnancy. The hormone FSH got a significant decrease in the three groups compared with a control (P ? 0.01), lowest level was in the third trimester of pregnancy.Estrogen level showed a significant decrease (P ? 0.01) for a non pregnant women with UTI compared with a control, and show a significant increase in the level of the two sets of hormone - positive pregnant non - infected, and the highest increase was in last third of Pregnancy in both groups. The progesterone showed a significant increase in the level of the pregnant infected and pregnant non - infected (P ? 0.01) compared with a control group. While in non - pregnant, there is no significant difference. The testosterone level increased significantly (P ? 0.05) in the three groups compared to control group. the level Interleukines, Tests showed that a significant increase(p ? 0.01) in the level of interleukin - 2 (IL - 2) for a pregnant women with (UTI) compared with a control and a significant decrease in the last third of pregnancy in pregnant women which is non infected compared to the rest of the gestation periods, and for the level of interleukin - 4 The present study showed a significant increase (p ? 0.01) in the level of interleukin - 4 (IL - 4) for a non - pregnant women with (UTI) compared with a controlThe highest level reached by IL - 4 was in the last third of pregnancy in the non - infected at (p ? 0.05), interleukin - 6 has a significant decrease for the two sets of pregnant women with (UTI) and non - infected, compared with the control group and the absence of significant differences between the two sets of pregnant women infected and non - infectedRegarding level of C - reactive protein there is a significant increase (P ? 0.01) in the two groups of pregnant and non pregnant women compared with a control, and significant differences between control group and a group of pregnant non - infected.

دراسة وبائية على طفيلي المشعرات المهبلية Trichomonas vaginalis وبعض الجراثيم المسببة للامراض المنقولة جنسيا وتاثير بعض المستخلصات النباتــيـــــة على نمو الطفيلي في الزجاج في مدينة كركوك == Epidemiological Study On Trichomonas Vaginalis & Some Associated Bacteria That Causing Sexual Transmitted Diseases & Effect of Some Herbal Extraction On The Parasite In Vitro In Kirkuk City

Author name: محسن عز الدين سليمان
Supervisor name: ابراهيم شعبان داوود | محمد عبد العزيز قادر
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: بلغ العدد الكلي للعينات التي جمعت وفحصت للفترة من بداية شهر تموز 2007 الى نهاية شهر مايس 2008 (2345) عينة لكلا الجنسين مــن مستشفيات كركوك للتحري عن وبائية طفيلي المشــــــعرات المهبليـــــــــة Trichomonas vaginalis وبعض الجراثيم المسببة للامراض المنقو | A total (2345) specimens were collected & examined from both sexes, from the beginning of July 2007 till the end of May 2008 attending Kirkuk hospitals for epidemiological study of Trichomonas vaginalis & some sexual transmitted microorganisms. The vaginal examinations were done by gynecologist, two high vaginal swabs were taken from (250) women & a cervical swab from some women with vaginal discharge. General urine examination & blood group with Rh factor were performed for each woman with vaginal discharge. Direct microscopic examination, staining & culture of swabs were performed on different methods for diagnosis of Trichomonas vaginalis, followed to diagnose Candida albicans, Neisseria gonorrhoeae, Chlamydia trachomatis & Treponema pallidum as well as some other bacterial groups. The rate of Trichomonas vaginalis was (2.8%) & Neisseria gonorrhoeae (0.8%), while no Chlamydia trachomatis & Treponema pallidum recorded in this study. The highest rate of Trichomonas vaginalis was among the age group between 15 - 29 years (3.6%), the rate of Trichomonas vaginalis infection was higher among illiterate (5.7%) than educated ones. The highest rate of Trichomonas vaginalis was recorded among those with blood group O & Rh (+ve) (3.8%). The same result was found regarding other microorganisms in both sexes. Statistically the results were not significant.The rate of infections in females was higher than males in all infection except Neisseria gonorrhoeae was recorded highest rate in males(35%). The percentage of mixed infections with Trichomonas vaginalis was Candida albicans (1.6%) & B - hemolytic streptococcus (0.4%). The maximum infections rate in vaginal swabs were Staphylococcus aureus (42.3%) & lowest rate of Neisseria gonorrhoeae (2%). Regarding the distribution of microorganisms isolated from urine samples of females suffering from vaginal discharges, the highest rate of infection was Eschirechia coli (22.2%) & lowest rate of Neisseria gonorrhoeae (2%) respectively. The rate of isolated microorganisms from urethral discharge of males were Neisseria gonorrhoeae (35%) & Escherichia coli (5%). Antibiotics Ciprofloxacin & Amikacin showed good inhibition activity against all pathogenic microorganisms. Regarding the effect of different concentration of metronidazole & medical herbs to the Trichomonas vaginalis invitro, metronidazole showed clear effective inhibition at concentration (1.25%) during 24 hours. The inhibitory effect of Zea mays (both alcoholic & watery extractions) was greater than Apium graveolens & Foeniculum vulgare. At concentration (2.5% & 5%) of Zea mays in both extractions was almost identical to metronidazole. Six solvents were used by thin layer chromatography (TLC) in order to separate alcoholic & watery extraction of medical herbs, only three of them A, B & F gave good results in separating herbal extraction with differing in its component. By measuring relative flow rate (Rƒ) of all compounds that separated and determind. The chemical compounds of each herbal extract were established & included alkaloids, flavonieds, amines, pigments, oils, carbohydrates, phenols, purines & tanines.

تقييم استخدام الانترليوكينات في تشخيص تسمم الدم الجرثومي لدى الاطفال حديثي الولادة في تكريت == Evaluation Uses The Interleukines For Diagnosis of Neonatal Sepsis In Tikrit

Author name: هبة حازم صالح
Supervisor name: موسى جاسم محمد الحميش | عاشور رفعت سرحت
General topic: Biology
Specific topic: Zoology
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: ان الهدف الرئيس لهذه الدراسة هواستخدام الانترلوكيناتInterleukin - 8, Interleukin - 10 (IL - 8, IL - 10) كعلامة تشخيصية للتسمم الدموي الجرثومي لدى حديثي الولادة. تضمنت الدراسة 45 عينة دم من الاطفال حديثي الولادة الراقدين في ردهة الاطفال في مستشفى تكريت الت | The main aim of this study was uses the Interleukines IL - 8 and IL - 10 as diagnosis marker for neonatal sepsis. This study involved 45 blood samples, were taken from neonates admitted to the pediatrics - neonatal wards at Tikrit Teaching Hospital who were clinically diagnosed as sepsis for the period from September 2011 until March 2012. The ages of neonates were 1 - 30day, and this blood samples were taken for the purpose of determining the levels Interleukines IL - 8 and IL - 10 and identify (WBC and C - reactive protein). Seventeen samples were taken from the blood of neonates without of septicemia as a control group samples. The results of the current study showed non significant differences in total white blood cells count for patients with sepsis when compared with a control group. and showed non significant differences in total white blood cells count for patients with sepsis as gestation birth, sex and birth weight compared to the control group. Regarding level of C - reactive protein there was no significant differences between the control group and the patients with sepsis as gestation birth and sex. and showed a significant differences in C - reactive protein level for patients compared to the control group as birth weight. The Interleukine levels showed that a significant increase in the level of interleukin - 8 (IL - 8) for all patients mals and femals, preterm or full - term and who there weight < 2.5 or >2.5kg compared with a control group. this results showed that the relationship between IL - 8 and WBCs was not significant differences at (p<0.05). and for the level of interleukin - 10 the present study showed a significant increase for patients with sepsis as gestation birth, sex and birth weight compared to the control group, the relationship between IL - 10 and WBCs was not significant differences at(p<0.05). The relationship between IL - 1o, IL - 8 and WBCs was not significant differences. when comparison The results as end result for sepsis show is the absence of significant differences (p<0.05) for patients who became good and who had complications compared with the control group. At last, the study concluded that the Interleukines IL - 8 and IL - 10 levels increase in early time for Inflammation ;So its agood diagnosis marker for neonatal sepsis.

تاثيـر المستخلص الكحولي لاوراق نبات ذيـل العقرب Heliotropium europium وفيتامينC في بعض الجوانب الفسلجية والكيموحيوية لذكور الجرذان المعرضة للكرب التاكسدي == Effect of Heliotropium Europium Leaves Alcoholic Extract And Vitamin C In Some Physiological And Biochemical Parameters of Male Rats Exposed To Oxidative Stress

Author name: صفا احمد عبد القادر محمد دنيدل
Supervisor name: صاحب جمعة عبد الرحمن | عويد هيدان الخزرجي
General topic: Biology
Specific topic: Zoology
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: هدفت الدراسة الحالية الى معرفة تاثير المستخلص الكحولي لنبات ذيل العقرب Heliotropium europium في وزن الجسم وعدد من المتغيرات الفسلجية والكيموحيوية كتركيز الهيموكلوبينHaemoglobin (Hb) وحجم كريات الدم المرصوصةPacked cell volume (PCV) والعدد الكلي لخلايا الدم | The aim of the present study was to investigate some effects of the alcoholic extract of Heliotropium europium in body weight and some of physiological and biochemical parameter such as concentrations of Hemoglobin (Hb), Packed Cell Volume (PCV) and the total count of leukocytes (WBCs) and concentrations glutathione (GSH), Malondialdehyde (MDA), Peroxynitrite radical (ONOO - ) , Superoxide dismutase enzyme (SOD), glucose, Total Cholesterol (TC), Triglycerides (TG), High density lipoprotein (HDL - C), Low density lipoprotein (LDL - C), Very Low density lipoprotein (VLDL - C), Atherogenic indices levels, Total protein, Albumin, Globulin, Urea and Uric acid in serum and histological changes in liver of male rats that exposed to oxidative stress induced by hydrogen peroxide H2O2(0.5%) with drinking water for (30) days, and comparison these effects with the effects of the vitamin C as well known antioxidants(250 mg/kg of body weight), for the purpose of this study preliminary experiments were carried out to find the most effective does of plant extracts and it was found that this does was (300 mg/ kg) body weight.The study included(35) male albino rats, (4 - 6) months ages and(245 - 280)gm weight were divided to (7) groups both group with (5) rats as follows : Control group, hydrogen peroxide (H2O2) group, Heliotropium extract group, H2O2+ Heliotropium extract group, Heliotropium extract + vitamin C group, H2O2+ Vitamin C group, H2O2+extract of Heliotropium+ vitamin C group.The results showed : 1. The animal under oxidative stress by hydrogen peroxide caused a significant decrease (p? 0.05) in body weights and values of Hb , PCV, GSH, activity of SOD enzyme, HDL - C, total protein, albumin, globulin and compared with the control group. While it caused a significant increase in WBCs, MDA, ONOO. - , glucose, cholesterol, TG, LDL - C, VLDL - C, Atherogenic indices levels(I, II, III) and urea compared with the control group.2. The treatment of animals exposed to oxidative stress induced by hydrogen peroxide with extract of Heliotropium europium showed significant increase in the weights of animals, Hb, PCV, GSH, activity of SOD enzyme, HDL - C, total protein, albumin and globulin compared with a hydrogen peroxide group. While this treatment showed significant decrease in WBCs, MDA, ONOO. - , glucose, cholesterol, TG, LDL - C, VLDL - C, Atherogenic indices levels (I, II, III), and urea when compared with a hydrogen peroxide group.3. Compared with a hydrogen peroxide treatment of rats exposed to oxidative stress by hydrogen peroxide with vitamin C, extract of plant and vitamin C with hydrogen peroxide group showed the results were similar to the results of treatment plant extract with hydrogen peroxide group.4. The treatment plant alcoholic extract and vitamin C showed a significant increase in the level GSH, MDA, activity of SOD enzyme, HDL - C, globulin compared with the control group. And showed a significant decrease in animal weights, WBCs, ONOO. - , LDL - C, VLDL - C and urea compared with a hydrogen peroxide group. While not shown significant differences in the values of Hb, PCV, glucose, cholesterol, TG, Atherogenic indices levels total protein and uric acid compared with the control group.5. The treatment alcoholic plant extract group showed increase in the weights of animals and values Hb, PCV, WBCs, GSH, MDA, Triglyceride, VLDL - C, Atherogenic indices levels, globulin and urea compared with the control group. Showed significant decrease in the HDL - C and albumin compared to the control group. while did not show significant difference in ONOO. - , activity SOD enzyme, glucose, cholesterol, total protein and uric acid compared the control group.6. The most important histological changes that have occurred in this study have showed treatment hydrogen peroxide compared with a control to swelling hepatocytes with necrosis each and the small size of the nuclei with swelling sinusoid, and the treatment of animals exposed to oxidative stress plant extract has showed a lack of clarity within the cells in hepatocytes necrosis and swelling sinusoid compared with a hydrogen peroxide while the treatment of animals exposed to oxidative stress with vitamin C showed sinusoid constriction and showed treatment alcoholic plant extract and vitamin C for the animals exposed to oxidative stress loss nucleus cells with the difficulty of distinguishing sinusoid.

تحديد مقاومة بعض الجراثيم المعزولة من اخماج في مستشفى تكريت التعليمي للمضادات الحيوية وعلاقتها بالبلازميدات == Determination The Antibiotic Resistance of The Some Isolated Bacteria From Tikrit Teaching Hospital Infections And Their Relationship With Plasmids

Author name: شيماء عبد محمد علي
Supervisor name: وعد محمود رؤوف | خالد عمر محمد علي
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: جمعت (140) عينة من المرضى الراقدين لثلاثة ايام فاكثر في مستشفى تكريت التعليمي للمدة من 1 - 8 - 2009 ولغاية 1 - 12 - 2009 بهدف تحديد العزلات الجرثومية الاكثر شيوعا ضمن اخماج مستشفى تكريت التعليمي ومقاومة هذه العزلات للمضادات الحيوية ودراسة العلاقة بين مقاو | One hundred fourti samples were collected from hospitalized patients for three days or more in the Tikrit Teaching Hospital during the period from 1/8/2009 until 1/12/2009 to determine the most commonly isolated bacterial from nosocomial infections in Tikrit Teaching Hospital and their resistance to antibiotics and to study the relationship between antibiotics resistance and plasmid content in the most commonly isolated bacteria and to evaluate the prevalence of these plasmids in the hospital environment. These samples include : (75) Urine, (28) stool, (28) of wound swabs and (9) sputum samples.The incidence of nosocomial infection was (45.7%) of them, urinary tract infections were (50.7%) from (75) samples, followed by wound infections (42.9%) from (28) samples, gastrointestinal infections (39.3%) from (28) samples then pneumonia (33.3%) from (9) samples the nosocomial infections were more common in females and age group 51 - 89 years. Different bacterial species were isolated in this study and the results showed that the most commonly isolated type was Citrobacter koseri in a proporation (21%) of urinary tract infections, followed by the following bacterial species : Escherichia coli (15.7%), Staphylococcus schleiferi (10.5%) , Staphylococcus epidermidis (7, 8%), Proteus vulgaris, Staphylococcus saprophytica, Klebsiella ozaenae, Klebsiella ornithindytica in aproportion (5.2%) for each, Enterobacter aerogenes, Providencia stuartii, Staphylococcus warneri, Staphylococcus aureus, Proteus mirabilis in aproportion (2.6%) for each.Citro. koseri were also the most common in wound infections (25%), followed by the following bacterial species : E.coli (16.6%), P.mirabilis (16.6%), Staph.schleiferi, P.vulgaris, Prov.stuartii and Prov.rettgeri in aproportion (8.3%) for each. Citro.koseri were also the most common in the gastrointestinal tract infections in a proporation (36.3%), followed by E.coli (18.1%), S.flexneri (18.1%), P.mirabilisc, Prov.stuartii, Prov.alcalifaciens in a proporation (9%) for each. . While Strept.pneumoniae was the most common(66.7%) in penemonia cases followed by K.pneumoniae (33.3%).The isolated bacteria were tested for antibiotics susceptibility and it has been found that most of these isolates showed high resistance to the studied antibiotics other than Prov. Stuartti was sensitive to all antibiotics except of Rifampicin and Tetracycline. As well as bacterial species belonging to the genus Staphylococcus were sensitive to Amikacin, Staph.aureus was also sensitive to Nitrofurantoin while Staph.warneri was also sensitive to Ciprofloxacin and Nitrofurantoin also Enterobacter aerogenes was resistant to all antibiotics except for Ciprofloxacin and Nitrofuratoin. Eleven of most commonly isolated bacteria which were multidrug resistant were chosen for the curing experiments using SDS to study the relationship of their plasmids with resistant to antibiotics. The results showed that the cured cells of E.coli lost their resistance to Tetracycline Ciprofloxacin and Cefotaxime which Citro.koseri lost their resistance to Tetracycline while K. ornithinolytica lost resistance to both antibiotics Tetracycline and Ciprofloxacin either P.mirabilis lost resistance only to tetracyclin as well as Strept.pneumoniae lost their resistance to Cefotaxime, tracycline and Ampicillin. Which may mean that the genes responsible for resistance to these antibiotics are located on plasmids as proven by the results of electrophoresis.Electrophoresis results also showed that all studied bacterial isolates had the same plasmid profile which may regarded as an epidemiological pointer that plasmids are conjugative which have the ability to transfer antibiotic resistance to other bacterial strains of the same species or to other different bacterial species.

دراسة بعض الصفات الفيزيائية والكيميائية والحياتية لمياه نهر دجلة ضمن مدينة تكريت == Study of Some Physical, Chemical And Biological Properties On Tigris River In Tikrit City

Author name: بشار طارق اسماعيل هلال الشنداح
Supervisor name: رياض عباس عبد الجبار
General topic: Biology
Specific topic: Plant
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تهدف الدراسة الحالية للتعرف على بعض الجوانب الفيزيائية والكيميائية والحياتية لمياه نهر دجلة ضمن مدينة تكريت ولمياه المخلفات السكنية في محطة المعالجة.جمعت العينات لمدة سبعة اشهر اعتبارا من شهر تشرين الثاني 2006 وحتى نهاية شهر ايار 2007 وبمعدل مرتين شهريا | The present study aimed to find out some physical, chemical and biological aspects of Tigris river passed in Tikrit - city, and population residence in the treatment unit.Samples were collected over the period of seven months during November 2006 to the end of May 2007 biweekly. Four stations were selected for sample collections over the distance of 25 km. Two stations were in Tigris river and two were in the treatment unit. The first selected station was close to the old water purification unit in Tikrit - city. The second station was selected at the treatment unit in AL - Dubaee before the mechanical treatment unit. The third station was selected at the treatment unit after the biological treatment. The fourth station was selected in Tigris river about ten km south of the treatment unit at AL - Dijla town. The study involved an investigation of some physical, chemical and biological aspects of Tigris river and the residence waste discharges to the river. This included air and water temperature, conductivity, PH, dissolved oxygen, BOD, Turbidity, total hardness, calcium and magnesium hardness, chloride, total alkalinity, nutrients (i.e., phosphate, nitrite, silica) , and the concentrations of heavy metals (i.e., cadmium and cupper). The biological factor included the total bacteria counts cultured on nutrient agar. Results of the study clarify the close relation between air and water temperature which varied (8.5 - 36) C0 and (9 - 27) C0 respectively. Conductivity values were relatively high and ranged (128 - 3530) microsemens/cm. River water almost was alkaline during the period of the study. PH values of the first and fourth stations were close in the river, although a value of 6.12 was recovered at station on during May. In all stations, PH values ranged (8.8 - 6.12). River water has a good ventilation, dissolved oxygen values ranges (8 - 11) mg/L at the first and the fourth stations, while these values were (0.1 - 1.34) mg/L at the second and third stations. Mean dissolved oxygen values pointed out the availability of good oxygen concentration for the continuity of living organisms which ranged (0.3 - 5.8) mg/L in Tigris river, while BOD values at the stations second and third were high and ranged (5.6 - 131.2) mg/L. Turbidity was high during April at the stations one and four and ranged (2.03 - 788) NTU in all stations. Total hardness in Tigris river was slightly low - very hardness and ranged (120 - 360) mg/L at the first and fourth stations, while these values were (180 - 480) mg/L at stations two and three.The high total hardness values coincided with the high conductivity values. Concentrations of calcium ions responsible on the calcium hardness ranged (211.4 - 120.7) mg/ L was higher than magnesium ions responsible for magnesium hardness range (5 - 265) mg/L.Records of chloride ions ranged (14.16 - 169.99) mg/L at all stations. However, chloride values at stations first and fourth were among the allowed world values and ranged (14.16 - 42.48) mg/L.Alkalinity values were due to the carbonate and bicarbonate and ranged (35 - 725) mg/L.phosphate values ranged (0.168 - 25.2) µg phosphor atom - phosphate/L. Low nutrient values recorded in Tigris river, while the highest values recorded at second station (treatment unit).Nitrite values fluctuated at all stations and ranged (0.078 - 14.778) µg Nitrogen atom - Nitrite /L. Silica values ranged (648 - 5961.6) µg silicon atom - silica /L. Heavy metals values (Cadmium and Cupper) clarify that, Cadmium were (un sensible), except one value (0.008) mg/L that recorded at November, and un sensible for Cupper.Results of the statistical analyses of all involved factors showed significant differences at stations first and second, and between the first and third stations as well as the fourth station. While there was no significant differences between the second and fourth stations. And no significant differences between stations first and fourth.Total bacterial counts indicated high bacterial counts, and the average of total bacterial count ranged (2.605X105, 5.44X108, 2.265X108, 2.385X105) / ml at the four stations respectively, so the Tigris river considered pollutant - very pollutant.

دراسة بيئية ونوعية لمشروع تصفية ماء الكرخ في الطارمية == An Ecological And Qualitativ Study For Al - Karkh Drinking Water Plane At Al - Tarmia

Author name: احمد توفيق احمد المشهداني
Supervisor name: رياض عباس عبد الجبار
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: نظرا لما لمياه الشرب من اهمية كبيرة في حياة الانسان وضرورة ان تكون هذه المياه ضمن مواصفات ومعايير خاصة, فضلا عن ظهور العديد من مشاكل تلوث مياه الشرب في مختلف مدن العراق. دعت الحاجة لاجراء هذه الدراسة لبيان مدى صلاحية مياه الشرب المنتجة من مشروع تصفية ماء | Owing to the drinking water of great importance in human life and it is important to be under the specifications and standards, as well as the emergence of many of the problems of contamination of drinking water in various cities of Iraq. Needed to conduct this study to demonstrate the viability of drinking water produced from the project filter water Karkh (which is the largest project the liquidation of water in the Middle East, it is feeds many of the cities of Baghdad, on the side of Karkh) and compare the specifications of Iraq and the global standard for drinking water in order to investigate the potential weaknesses in the project and study the possibility of the development of such large projects as a first step to improving the situation of drinking water in Iraq. So were the tests, physical, chemical and biological common to drinking water in the project and divided into four main stations, namely, (station socket raw water, sedimentation basins secondary sand filters, pumps, distribution of drinking water), including nine stations secondary starting of the month of October - 2010 to July - 2011.The results showed high values of turbidity, and electrical condactivety and pH, and dissolved oxygen, and biological dimond of oxygen, and alkalinity, and total hardness, calcium hardness, magnesium hardness, chloride, phosphate, nitrite, silica, fluoride, sodium, potassium, and heavy metals, namely, (iron, copper and cadmium, nickel, lead, chromium and zinc), and the total numbers of bacteria, and total coliform bacteria, the fecal coliform bacteria and E. Coli in drinking water for most of the stations in the Alsta spring and low concentrations in summer and autumn.Values for water temperatures between (4 - 37.5) c, as recorded differences significant between the months of winter and spring, summer and autumn, and the values of brownish discharge ranged between (250 - 0.3) NTU as it showed the differences of the moral high values of turbidity in the winter and fall in the summer and fall in each stage of the treatmant with it, and the values of electrical conductivity was between (520 - 895) Micro Siemens / cm 3. The water Alnehrualcherb in the project was registered as a weak base pH values between (9.7 to 1.7), and it was well ventilated, as the oxygen values were high in the winter months registered clear monthly variation ranging between (5.14 - 2.3) mg / L, either values biologicaldimond of oxygen were values between (4.4 - ND) mg / L and found that basal total stations ranged from every stage of the liquidation potential in increasing concentrations of dissolved oxygen, between (160 - 100) mg / L, and found that drinking water hardness too, as that the values of total hardness ranged between ( - 215 335) mg / L as suited their values directly proportional to both the calcium and magnesium hardness ranging between (197 - 139) and (31.472 - 16.128) mg / L, respectively, while for the values of the chloride ranged between (69 - 25) mg / l, while the fluoride has recorded values ranged between (15 - 0.05) mg / L, with values ranging between sulfate (169.2 - 90) mg / L, free chlorine residual values ranged between (4.18 - 1.1) mg / L, and materials for suspended solids ranged values between (84 - ND) mg / L proven contribution stages of liquidation, all in the rates of removal of different, either TDS fluctuated values between (545 - 315) mg / L, with log Aluminum values ranged between (0.98 - 0.01) mg / L, but for the potassium values ranged between (3.2 - 2.15) mg / L, and sodium values ranging between (6.31 to 3.21) mg / liter.As for the concentrations of heavy metals did not detect any significant concentration of heavy metals measured except for iron ranging between concentrations (9.2 to 8.1) mg / L, so after the industrial plants that contain the droppings of these metals. The nutrient values were recorded between nitrite (0.024 - 0.001) mg / Trosgelt nitrate values between (1.7 - 0.16) mg / l, recorded between ammonia (0.54 - ND) mg / L, placed at either phosphate values ranged between (0.26 - ND) mg phosphorus atom - phosphate / L, while for the values of silica fluctuated between values (5.12 to 5.1) mg atom of silicon - silica / liter. As for the study of signs bacteriological ranged the total numbers of bacteria in air through (24) hours between (555 - 0) × 10 cells / 1 ml, and ranged in numbers for a period of (48) hours between (1186 - 0) 10 × cells / 1 ml, while for the numbers of total coliform bacteria fluctuated between (1700 - 0) cells / 100 liters, and fecal coliform bacteria, they ranged (1100 - 0) cells / 100 ml, and the E.coli bacteria, the numbers fluctuated between (1100 - 0) cells / 100 ml. For to match the drinking waterproduced from the projects liquefaction research found matching the values and concentrations of brownish, and connectivity electrical and pH, and dissolved oxygen, and the requirement is vital for oxygen, and basic college, and chloride, and ten college, and magnesium, nitrite, phosphate, and the total numbers of bacteria, and the numbers of total coliform bacteria, and fecal coliform bacteria and E Coli, and silica with the standard specifications for drinking water the Iraqi and global at all stations.While the study results show non - conformity of calcium concentrations in this study with the standard specifications for drinking water of Iraq and the world.

تقييم فعالية مستخلصات مستنبت القمح Triticum aestivum في بعض الانواع البكتيرية المسببة لاخماج مختلفة == Evaluation The Activity of Germinated Wheat Extractions Triticum Aestivum On Some Bacterial Species That Cause Different Infections

Author name: اسماء عيسى محمود
Supervisor name: رشيد حميد حسن
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: The study had included isolation and diagnosis of three hundred samples for both sexes their ages were between (1 - 60) years of the inpatients and outpatients in Tikrit Teaching Hospital and General Samarraa Hospital during the period from 1/9 / 2011 to 1/3/2012. The infections were distributed among the burns infections, the wounds, urinary tract infections (UTI) and female genital tract infections (High vagina). This study clarified that (192) sample from the total samples showed bacterial growth in percentage 64%. The results of this study indicated to the proportion 58% with (UTI) by (87) bacterial isolation were produced from (150) isolation. The percentage of burns infections were 81.81% from (55) sample which distributed among the first, second and third class burns. The burns of second and third class gave bacterial growth in a proportion 100% while the first class burns gave 64.28%. Concerning the wounds infections, bacterial growth was 62.9% from (62) sample. The infections proportion of (H.V) was 63.63% from (33) sample. The results of the current study showed that the infections in females were higher that in males with respect to (UTI), while there was not considered difference between the males and females about the burns and wounds infections. Differently, the (H.V) infections were just for the females in the ages between (18 - 45). It was clear according to the results of diagnosis that Escherichia coli had the highest proportion of isolations 25.52% from the total isolates followed by Staphylococcus aureus 18.23% Pseudomonas aeruginosa 15.63%, both Klebsiella pneumoniae and Proteus miribilis 10.42% Staphylococcus epidermidis 4.69% Citrobacter freundii 4.17%, Staphylococcus saprophyticus 3.64% both Serratia marcescens and Enterococcus faecalis 2.6%, Streptococcus pyogenes 1.56% and Staphylococcus intermedius had the least percentage 0.5% by one from (192) isolate. The sensitivity of these isolates was tested by (10) antibiotic which included quinolones, aminoglycosides, beta - lactams and tetracyclines antibiotics. Among these antibiotics imipenem and ciprofloxacin were more effective, while the other antibiotics were different either active or not active at all.The wheat germinating was used in this study with the ages (36 - 48) hour and its activity was tested by using some bacterial kinds. After completing several quantitative and qualitative tests on this germinating, the carbohydrates proportion was between 67 - 70%, the protein proportion by folin method was between 10 - 8%, the lipids proportion was 1.5%.Some mineral elements in germinated wheat were estimated which include Calcium, Iron, Magnesium, Potassium, Copper, Manganese, Zinc, Selenium, and Sodium element, active materials were also estimated in the wheat germinating extracts which had inhibitory effect on the microbiology. It was clear by the tests that the germinating contains a lot of Phenolic compounds, Terpeniods, glycosides and steroids. These materials were extracted by using some extracts included the cold water extracts and hot water extracts, alcoholic extracts (ethanol and methanol) and phenolic extract. All the extracts prepared in concentrations (25, 50, 100, 200) mg/ ml. This study showed that the phenolic extract had high effect on the bacterial kinds in comparison with the methanolic and ethanolic extracts at (200) mg/ ml concentration while the cold water extract had less effect on these bacterial isolates in comparison with the above extracts, finally the hot water extract had no inhibitory effect against any isolated bacterial type with all the concentrations. The inhibitory action of these extracts was compared with some antibiotics as a control sample. These antibiotics represents different groups of antibiotics and which contained Imipenem, Ciprofloxacin, Gentamicin, Ceftazidime in addition to Augmentin.In this study the minimum inhibitory concentration (MIC) of the fenolic, alcoholic and watery extracts was determined and the concentration was different according to the extract type, the bacterial type and the isolation site.

التحري عن مؤشرات وراثية وفسلجية لتحمل الجفاف لصنفين من الحنطة Triticum aestivum L. == Investigation of Genetic And Physiological Markers For Drought Tolerance of Two Cultivars of Wheat (Triticum Aestivum L)

Author name: اشواق ابراهيم بشير العبيدي
Supervisor name: شاكر مهدي صالح | عقيل حسين العاصي
General topic: Biology
Specific topic: Plant
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تم في هذه الدراسة البحث عن امكانية ايجاد مؤشرات وراثية ترتبط بمؤشرات فسلجية وذلك من خلال الدراسة على مستويين : ا - المستوى الفسلجي : زراعة صنفين من الحنطة الناعمةL Triticum aestivum في حقول كلية الزراعة/ جامعة تكريت لموسم النمو 2010 - 2011، وذلك بزراعة بذو | This study was conducted to investigate a genetic marker linked to physiological marker through a design an outdoor experiment which includes two parts : - 1 - Physiological part : Included planting two cultivars of soft wheat (Triticum aestivum L.) in the fields of the college of Agriculture, university of Tikrit, during growth season (2010 - 2011). The stage of work had been done by cultivated the seeds of two cultivars of wheat (Sham - 6, Abu - Ghraib) in pots, when they reached the stage of (4 - 5) leaves it had been exposed to two dryness periods 7 and 10 days. It spread with growth regulator Indole Acetic Acid (IAA) (150 ppm) to know which type is more resistance for dryness through estimation of proline concentration as a physiological marker and to indicate the effect of IAA to reduce the dryness effect in some features of growth physiological function and yield component according to complete Randomize Design (CRD). Some markers of drought tolerance have been estimated including : proline content, chlorophyll concentration and the degree of membrane stability then plants were left for harvesting period. The results of the outdoor experiment were : 1 - Exposure the plants to dehydration periods during their growth caused reducing of the most studied characters (leaf area, plant height, tillers number, concentration of chlorophyll A and B and the total chlorophyll), while there was an increase in the content of the proline and the injury index of plasma membrane. 2 - Some characteristic did not showed effect by drought periods (dry weight of plant, number of days of flowering, spikes number, spike length, number of grains/ spike and weight of 1000 grains).3 - Sham - 6 cultivar was superior than Abu Ghraib - 3 in three characteristic (number of days of flowering, leaf area, weight of 1000 grains), while Abu Ghraib - 3 cultivar was superior in the grains number /spike than sham - 6 cultivar.4 - The concentration of IAA (150 ppm) had a different effect. It had a positive effect in the decrease drought for some characteristic (proline content, injury index of plasma membrane, leaf area, plant height, dry weight, spike number and grains yield).2 - Genetic part : DNA has been isolated from fresh leaves of two types of wheat, arrange yields of DNA were in the range of (40 - 150) micro gram per 1.5 gram of plant leaves, and the purity ranged between (1.2 - 1.7). The reaction PCR was done by using 11 primer of the Random Amplified Polymorphism DNA (RAPD) in addition to two pair of specific primer to detect the genes which are responsible for coding of two enzymes which involved in anabolism and catabolism of proline. The results obtained are as follows : - 1 - Five primer of RAPD did not appear any binding site (OPA - 18, OPL - 20, OPA - 11, OPQ - 17 and OPH - 14).2 - Six primer of RAPD have appeared main and monomorphic bands (OPO - 16, OPH - 07, OPN - 16, OPM - 20, OPJ - 13 and OPQ - 06), in addition to the absent of the other bands from some treatments. 3 - The two pair of specific primer have given three monomorphic bands with different molecules weight.4 - The primer (OPH - 07) has appeared a unique band with molecular weight (1670) pb for Sham - 6 cultivar during second drought period (10 days without hormone). These results obtained from this study were identical with physiological part in the ability of both types to drought tolerance

دراسة تاثير مستخلصات نباتي القرنفل والخروع على نمو بعض الفطريات المنتجة لانزيم البروتييز والمسببة لالتهاب الاذن الخارجية == Study Effect of Syzigium Aromaticum And Ricinus Communis Extracts On Growth of Some Fungi That Produce Protease And Cause Otitis Externa

Author name: بتول عمران ذيب
Supervisor name: خليل ابراهيم بندر النعيمي | ذكرى احمد حمادة
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: ضمنت الدراسة عزل وتشخيص الفطريات من الاذن الخارجية في مستشفى تكريت التعليمي - وحدة الاذن والانف والحنجرة والعيادات الخاصة في مدينة تكريت, خلال الاشهر تموز, اب وايلول 2008. جمعت 100 مسحة اذنية من المرضى المشخصة اصابتهم بالتهاب الاذن الخارجية الفطري من قبل | The study was included isolation and identification of fungi from the external ear in Tikrit teaching hospital, the unit of Ear, Nose, and throat (E.N.T.) and Privete clinics in the city of Tikrit during the months July, august, and September2008. 100 ear swabs were collected from the patiants whose diagnosed by Otologists with Otomycosis, and 50 ear swaps were collected from healthy persons in the same city and period time, These samples were examined by dierect microscopic examination treated with KOH 10% with hot flame to ensure the fungal infections, and Culturing on Sabouraud, s Dextrose Agar was done for isolation and identification of types of fungi which responseple for infection, The result of direct microscopic examination and culture showed that 76(76%) were positive for fungi, 64 were positive by direct microscopic examination and culture, and 12 posative in culture only. The most common fungi isolated from patient were Aspergillus Spp.36). (%22 followed by Candida Spp. (%67. 10), Trichophyton Spp(%6.5) , and Penicillium spp. (%3.94%), Aspergills niger appeared as the predominant fungi (%84. 36) followed by Aspergillus flavus (%36. 22), Candida albicans (42. 18%), Aspergills terrous (7.89%) , Candida kruzi, Penicillium spp (3.94%), Trichophyton mentagrophyte, Trichophyton verrucosum (63. 2%) and Trichophyton scheonlieni (%1.31), and no fungi were isolate from the healthy person. Higher percentage of fungi infection were recorded in female 46(60.52%) than male 30(39.47%). and the higher percentage for fungal infection was recorded in the age group (31 - 40) with percentage (23.68%). fungal ear infection was recorded with highest percentage in right ear 47 (61.84%), and for the left ear 29 (38.15%). and high rate of infection was recorded in august (73.69%), Higher percentage of infection was recorded in patient house wives (52.36%), the itching was the most commen symptom , The most habits used to clean the ear by dearty body (51.31%), Ahigher percentage (76.4%) recorded in non educated persons. The results of active ingredients test for Syzigium aromaticum and Ricinus communis revealed that clove dry flower buds contain Flavonoides, Glycosides, Tannins, Volatile oils, Terpenes, Resins, and Phenols, while castor leaves contain Flavonoids, Tannins, Volatile oils, Terpenes, Resins, Saponins and Phenols, pH of clove bud 4.3 and pH of castor leaves 5.8. Aqueous and alcoholic extracts were Prepeared, which belongs to Syzigium aromaticum and Ricinus communis under the study, commercial oil for clove and seed castor were used in the study, the effects of inhibition of Aqueous and Alcoholic extracts and commercial oil had been studied on the growth and protease production on the three fungi which were showed the high ratio appeared among the isolation sample under the study with 5 concentration 10, 20, 30, 40, 50 (mg /ml), and the affected rate of these extracts and oils under the study on fungi were varied. The relation between Aqueous extract of clove bud and fungi growing was increasing relation, While Alcoholic Extract and commercial oil showed preference in the inhibition of the isolated fungi grown while its effect on Candida albicans was increase with increase the concentration, The effect of aqueous and Alcoholic Extract of castor leaves on Moulds showed reverse effect. on the other hand the commercial oil effect was slight effect, and both extracts did not showed effect on the Candida albicans as well as the Commercial oil.When adding Clove Extracts and Commercial oil and in all concentrations dose not gave any production of the Protease enzyme in the solid media, whereas the extracts of castor leaves and commercial oil were showed effects on the production of Protease enzyme, and Aspergills niger was more effected and these extracts did not showed an effect on the Candida albicans.Aqueous and Alcoholic extract and commercial oil to the Clove revealed higher influence rate than the effect of the Castor leaves extract , and in a different rate

التاثير الوقائي لعصير الليمون والمستخلص المائي للمعدنوس في منع التحصي التجريبي المستحدث باستعمال الاثيلين كلايكول وتقليل السمية الكلوية المستحدثة بالمضادين الحيويين جينتامايسين وفلوكساسين في الجرذان == The Protective Effect of Lemone Juice And Parsley Aqueous Extract In Prevent Experimental Urolithisis Induced By Ethylene Glycol And Decrease Renaltoxity Induced By Gentamicine And Floxacin Antibiotics In Rats

Author name: صبا خير الدين ابراهيم الطائي
Supervisor name: زيد محمد مبارك المهداوي | فدوى خالد توفيق
General topic: Biology
Specific topic: Zoology
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: هدفت الدراسة الحالية الى معرفة بعض التاثيرات الفسلجية والكيموحيوية والتاثيرات النسيجية للمستخلص المائي لنبات المعدنوس Petroselinum Sativum وعصير الليمون Citrus Linnaeu في كبح تكون حصى الكلى Renal calculi المستحدث بمادة الاثيلين كلايكول Ethylene glycol وال | The study aimed to searching the Physiological, Biochemical and Histological effects of the aqueous extract parsely of petroselinum sativum and lemon juice of citrus linnaeu in inhibition renal calculi formation which induced by Ethylene glycol and decreasing the nephrotoxity casead by the use of Gentamicin and Ciprofloxacin antibiotics. The study included number of Physiological and Biochemical parameters : total white blood cells count (WBCS), Hemoglobin (Hb), packed cell volume (PCV), Total cholesterol (TC), Triglycerides (TG), High density lipoprotein (HDL - C), Low density lipoprotein (LDL - C), Phospholipids, Atherogenic indices levels, Total protein, Albumin, Globulin, Blood Urea, Serum Creatinine, Glutathione (GSH), Maloddialdehyd (MDA), Aspartate amino transferase (AST) and Alanine amino transferase (ALT) concentration, beside the measurement of calcium ions, potassium ions, and magnisum ions concentrations in male and female rat serum which had been induced Oxidatiotion stress by use (0.05) hydrogen peroxide daily for (30)days.Also anather group of rats mouthy feeded with(100 mg/kg of body whight)Ciprofloxacin and the last group of rats feeded with (80 mg/kg of body wight)Gentamicin by muscular injection daily for (14)days. the active dose of petroselinum sativum (parsley) aqueous extract and Citrus linnaeu(lemon juice)had been demonstrated as (250 mg/kg of body whight)and(1.5ml/kg of body whight) successively.the rats distributed randomly (male and female)to (13) groups included (five rats for every group) : control group, Ethylene glycol (Eg)group, (Eg)+parsely aqueous extract group, (Eg)+lemon juice group, Hydrgen peroxide(H202)group, (H202)+ parsely aqueous extract group, (H202)+ lemon juice group, Ciprofloxacin+ parsely aqueous extract group, Ciprofloxacin+ lemon juice group, Gentamicin+ parsely aqueous extract group, Gentamicin+ lemon juice group.The results showed1 - the Treatment rats with Ethylene glycol caused high significant increase (P? 0.01)in concentrations of, TC, TG, Phospholipids, 2nd and 3rd of atherogenic indices, blood urea, ALP, serum creatinine, VLDL - C, AST, WBCs and MDA, while significant (P? 0.01) decrease had been happen in the concentration of albumin, globulin, total protein, HDL - C, GSH, ALT, Hb, Ca, Ma.2 - the Treatment rats with Ethylen glycol and lemon juice and parsley produced high significant increase(P? 0.01) in concentrations of albumin, globulin, total protein, HDL - C, GSH, ALT, Hb, Ca, Ma. while significant (P? 0.01) in concentrations of, TC, TG, Phospholipids, 2nd and 3rd of atherogenic indices, blood urea, ALP, serum creatinine, LDL - C, AST, Hb, K and MDA compared whith the E G onlay.3 - Induced oxidative stress by H202 caused high significant (P? 0.01) in the concentrations of TC, Phospholipids, 2nd and 3rd of atherogenic indiced blood urea, ALP, serum creatinine, k+, AST, LDL - C, MDA and Hb while significant (P? 0.01) decrease had been happened in the concentrations of albumin, globulin, total protein, HDL - C, GSH, ALT and 1st atherogenic indices.4 - the Treatment rats with (H202)whith lemon juice and parsely for rats produced high significant (P? 0.01) increase in concentrations of albumin, globulin, K+ total protein, HDL - C, GSH, AST. while Increase significant (P? 0.01) in concentrations ofTC, Phospholipids, 3rd of atherogenic indices, blood urea, ALP, serum creatinine, LDL - C, AST, Hb, K and MDA compared whith the(H202) Only.5 - The results of Treatment the animals with Gentamicin and Ciprofloxacin produced high significant (P? 0.01) in the concentrations of TC, TG, Phospholipids, Ist atherogenic indices blood urea, glubulin while the Treatment with Ciprofloxacin caused significant increase (P? 0.01) in concentrations of serum creatinine, VLDL - C, AST, WBCs, MDA, ALT, K+ and Hb.on the another significant decrease had been oecured in the concentrations of ALP, albumin, globulin while decrease of concentrations occurred when the animal had been treated with Gentamicin : total protein, HDL - C, GSH, Hb, Mg+and Ca++.6 - The Treatment by Gentamicin+ Ciprofloxacin+ lemon juice+ parsley to the rats when compares whith rats treated only antibiotics caused high significant (P? 0.01)increase in concentrations of albumin, , globulin, total protein, HDL - C, GSH, K+, Hb and ALP. while high significant decrease happened in the concentrations of total protein, MDA, Ist and3rd atherogenic indices, blood urea, TG, serum creatinine, ALT, MDA, and VLDL - C.7 - The Treatment with Ethylene glycol caused many histological changes like preipration of calcium oxalate in kidney tubules, necrosis, recrudesce of glomerales and caused degredation to the central vein, bleeding in kidney while the two types of antibiotics Gentamicin and Ciprofloxacin caused the same effects as ahave.8 - The action of lemon juice and parsely showed aprotection role against calcium oxalate precipitation and prevented the renal calculi formation or it aggregation in the kidney of the rats treated with Ethylene glycol.the action of that extract and juice were the decrease of oxidative stress damage induced by(H202)in the kidney tissue.

دراسة بعض المؤشرات الوراثية الخلوية لتاثير مادة الكلوروفورم على الفار الابيض Mus musculus L == A Study On Some Cytogenetic Indices For The Effect of Chloroform On The White Mouse Mus Musculus L

Author name: عمر رحيم خلف
Supervisor name: عادل فوزي شهاب
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: يستخدم الكلوروفورم على نطاق واسع في العديد من الصناعات مثل صناعة الورق والمطاط ومطافئ الحريق ومستحضرات التجميل ومواد التخدير. كما يستخدم كمذيب عضوي للعديد من المواد في المختبرات التعليمية والصناعية. وينتج الكلوروفورم من العمليات الايضية التي تقوم بها بعض | Chloroform is widely used in many industries, such as paper, elastic, fire extinguisher, cosmetics and anesthetic materials industries. It also used as an organic dissolvent for many materials in industrial and education laboratories. Chloroform is produced by The metabolism process of living organisms such as macroalgae and some fungus of highly organic matter soil. It can also be produced by process of water cholornation. Because of chloroform is widely used, and few studies achieved on cytogenetic level. Therefore The present study was conducted to identify The effects of chloroform on cytogenetic characteritis in laboratory mice.This study includes : 1 - A Study on Some Cytogenetic Indices For The Effect of Chloroform on The White Mouse.2 - Sperm abnormalities of male mice.The results of The present study showed that The chloroform has the ability to motivate chromosomal aberrations which were represented by (ring chromosom, chromatid break, and centromore break) in somatic cells in both males and females mice and in germ cells in males, The results revealed significant differences in The chromatid break and ring chromosome of both male and females mice, which injected with two concentrations (0, 025 & 0, 05 mg/kg) when compared with control group. It was found That there were asignificant differences in centromore break of females, injected with two concentrations of chloroform in comparison with control group, while non significant were differences observed in The males.Also the present study showed there were significant differences in The chromosomal aberration in The germ cells of males mice, injected with two concentrations (0, 025 & 0, 05 mg/kg) when compared with controlled group.The concentration (0, 05 mg/kg) was more influent in comparison, with The concentration (0, 025mg/kg). It was found that 30 days of injection is more influent in comparison with 15 day of injection, and males are more influenced than females.The present study recorded a significant differences in creating micronuclei in both male and female mice, injected by chloroform with a concentrations (0, 025 & 0, 05 mg/kg)in compared with control group.The result also revealed that The chloroform cause a significant decrease in The mitotic index of The somatic cells in both males and females, morever significant decrease in The mitotic germ cells of The injected males mice was found.An increase in The Blast index factor in The somatic cell in both male and female mice, injected by chloroform when compared with control group was similar result was recoded rgarding The Blast Index factor of The males germ cells. Regarding The sperm abnormality, the result showed significant differences in The sperm abnormality of males injected by chloroform with two concentrations (0, 025 & 0, 05 mg/kg) in comparison with The control group. The concentration (0, 05 mg/kg) was more influent than The concentration (0, 025mg/kg). Besides and the period 30 days of injection was more influent than the period 15 days.

دراسة عدد من المعايير الدموية والكيموحيوية وبعض الهرمونات لدى النساء العقيمات في محافظة صلاح الدين == Astudy of A Number of Hematological, Biochemical Parameters And Some Hormones In An Infertile Women In Salahaddin Governorate

Author name: لينا قيس ياسين عبد الرزاق
Supervisor name: زيد محمد مبارك المهداوي | بشرى محمد مجيد
General topic: Biology
Specific topic: Zoology
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: هدفت الدراسة الحالية الى تحديد عدد من العوامل المسببة للعقم لدى عدد من النساء في محافظة صلاح الدين. بدات الدراسة من ايلول (2008) ولغاية اب (2009), وحددت نسبة العقم الاولي (%55) والعقم الثانوي (%45) لعينة الدراسة التي شملت 400 عينة عشوائية للنساء المصابات | This study goaled to determine the causes of the infertility in a number of women in Salah - El - Din governorate. The study began in September (2008) until August (2009), the primary infertility was (55%) and the secondary infertility was (45%). The study sample, which included random sample of 400 infertile women aged (15 - 45) years. Whom reviews in Educational Tikrit Hospital and many external women clinics in Tikrit city. The studied samples are compared with 50 healthy women as a controlling group. The age groups of infected women are specified as highest average within (21 - 25) years then from (26 - 30) years then from (31 - 35) years respectively. The study included a cellular classification by using sex chromatin as an indicater of fertile degree (sex chromatin as a guide to classify women by examing Neutrophil polynucleated cells). So wamen were distributed to : Supper female (have 2 sex chromatin), Normal female (have 1sex chromatin) , and Abnormal female (with out sex chromatin).The results showded 4% and 44.75% and 51.25% respectively. Respect to sex chromatin the results revealed high significant levels (P <0.01) and (P <0.05) of prolactin and (LH) in cons. for Abnormal and Normal infertile women. While the same results and levels happened in FSH and Proges. Conc. In Abnormal and Normal infertile women in comparison with the controlling group.this study showed no significant at level (P >0.05) for Estrogen E2 besides there is no significant in comparing infertile women of Supper female with the control group. Concerning biochemical factors and their relation with sex chromatin, it is shown that there is a difference at (P<0.01) (P<0.05) for normal and a bnormal group in comparison with the controlling group, at level LDL, VLDL and total protein between high and low but there is no difference at level HDL, Tri and Chol The study of reproductive hormones (FSH) and (LH) (PRL) and (E2) had been measured at 13 - 14 days of the menstrual cycle of infertile women. And progesterone hormone conc. had been measured at 21 days of the menstrual cycle. The results showded high significant conc. of PRL, LH and Proge. at level (P<0.01) in comparison with control group while their was not significant changes when the infertile women with the control group for FSH and estrogen conc. Respect to the biochemical factors have shown through fat parameter cholesterol, triglyceride, low density lipoprotein - cholesterol very low density lipoprotein - cholesterol and total protein have shown high levels (P<0.01) for infertile women in comparison with control group. While high density lipoprotein - cholesterol and glucose conc. Albumin, conc. and globulin show no significant changes in comparison with the control group. Besides this study has revealed a high levels (P<0.01) at PCV % for normal female in comparison with infertile female but through Hb there is no significant differences. It is also shown that the average of women with poor ovarian is 69.25 % while the block of fallopian tubes line was 18.75% for the endometrosis 4% and unknown infertile female causes were 8%. Then results also show the average of 36% of infertile female who are infected with hyperprolactinemea, results show that they are about 23% who suffer from Polycystic ovary syndrome PCOS. Also the Body Mass Index (BMI) showed high significant level (P<0.01) infertile women compared with the control and the same results happened with the age.

دراسة تاثير النفط الخام وهايدروكسيد الامونيوم على بعض صفات الترب ونمو نبات الشعير الابيض == Study The Effect of Crude Oil And Ammonium Hydroxide On Some Characteristics of Soils And Plant Growth of Barley White

Author name: براء محمد ابراهيم
Supervisor name: جهاد ذياب محل الجنابي
General topic: Biology
Specific topic: Environment - Environmental pollution
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: شمل البحث دراسة انتشار المخلفات النفطية وهايدروكسيد الامونيوم في مقاطع لاربعة ترب مختلفة النسجة وهي التربة (المزيجية الرملية، المزيجية الغرينية، المزيجية الطينية 1، والمزيجية الطينية 2)؛ اجريت التجربة باستخدام صندوق مصنوع من الخشب الغير منفذ للماء؛ اذ قسم | Research involved studying the spread of oil residues and hydroxide ammonium in excerpts of four soils of different Texture, the soil (sand, gypsum, Loam, and clay); experiment was carried out using a box made of wood and non - port of the water; it was divided into four sections with barriers, wooden supported tin galvanized to ensure that Waste oil spread between the different types of soils, left central Hole made of tin and galvanized perforated and lined with container made of filter paper. Container filled with waste oil and allowed to seep into sections of the four soils through the holes, where waste oil was allowed to spread for 45 days, after which a series of analyzes were conducted to look for oil residue in the soil sections of the experiment. Then added ammonium hydroxide liquid substance in the central Jura and left for 45 days also were conducted after a series of analyzes to determine the changes and the effects on the soil material and components; was also white barley cultivation, After two months of growth, a set of analyzes were conducted Hordeun vulgare Barley and whose scientific name to find out the impact of oil on the chemical characteristics and uptake of heavy metals.Results were recorded the highest prevalence rate of the waste by 218.5 ppm in the Loam soil in depth 15 cm, while the lowest in the clay soil at the depth of 45 cm 44.8 ppm, while in the sandy soil did not happen the spread in the depths of 15 cm and 30 cm; also recorded the highest connectivity Power in the Loam soil 5.37 Micro Smenz / cm and recorded clay soil less connectivity, electric 1.81 Micro Smenz / cm; and when measuring the pH was recorded sandy soil 8.3, while they recorded the soil gypsum 7.5 As for the relative humidity in the soil recorded soil Loam the highest percentage is 93.55% in while the lowest percentage recorded in the soil moisture gypsum 57.90%. Were measured concentration of heavy metals such as cobalt, where the highest concentration in sandy soils 15.40 ppm while the lowest concentration him in the clay soil 7.10 ppm; and cadmium, where the highest concentration in soil gypsum 58.10 ppm in return was the lowest concentration of cadmium 14.60 part million in the clay soil; and when measured the highest concentration of lead found in the soil has Loam reaching 122.80 ppm was the lowest concentration of lead in soil clay 71.90 ppm.Reached the highest value of chlorophyll a and chlorophyll b in plants grown in clay soils (3.21, 2.96) Eq ml / g, respectively, while the highest concentration of phosphorus, chlorine, magnesium, sodium, calcium, and potassium in the soil clay (5.6, 15.9, 2.9, 16.4, 20.1, 46) ppm, respectively;As for the heavy elements (chromium, cobalt, cadmium, lead, and iron) have the highest concentrations in plants grown in soil gypsum, as was (0.98, 0.24, 0.70, 0.31, 14) ppm.While the lowest value for chlorophyll a and chlorophyll b in plants grown in sandy soil (1.62, 1.24) Eq ml / g, while the lowest concentration of elements (phosphorus, chlorine, magnesium, sodium, calcium and potassium) in plants grown in sandy soil reaching (4.2, 4.1, 1.2, 5.9, 1.2, 12.2) ppm, the lowest concentrations of heavy elements were recorded in the sandy soil as well as the (0.03, 0.12, 0.20, 0.20, 8.3) ppm.

دراسة مظهرية وتشريحية مقارنة لانواع في اجناس معينة من العائلة المركبة Compositae (Asteraceae) في العراق == A Comparative Morphological And Anatomical Study of Species In Certain Genera From The Family Compositae (Asteraceae) In Iraq

Author name: عمـر خليل جاسـم محمد العباسي
Supervisor name: خليل ابراهيم عباس الشمري
General topic: Biology
Specific topic: Plant
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تناولت الدراسة الحالية, دراسة مظهرية وتشريحية مقارنة لـ(10) انواع تعود لـ(9) اجناس من العائلة المركبة Asteraceae (Compositae) في العراق, كما وتبعت الاجناس قيد الدراسة الى عشيرتين Tribes : العشيرة الاولى : (Cardueae), وقد ضمت الانواع : (Arctium tomentosum, Ca | The current study dealt with the morphological and anatomical aspect that compared the (10) Species that followed (9) genera, These Species followed the family Compositae (Asteraceae) in Iraq. It also divided the genera under study to two tribes. The first tribe : (Cardueae), It included the Species : (Arctium tomentosum, Carlina vulgaris, Chardinia orientales, Cnicus benedictus and Notobasis syriaca). The second tribe : (Lactuceae), It included the Species : (Geropogon glabrum, Geropogon hybridus, Hedypnois cretica, Koelpinia linearis and Urospermum pecroides). The morphological study involved the roots according to the characteristics, the form and dimensions (the length and the diameter), the study of the stem by the character, the color and the indumentums, also the dimension (the high and diameter) it resulted good characteristics, enabled us to use it in isolation of the types. It is also discussed the leaf on the based of the quantifier and qualifier that involved the form of the leaf and the form of the peak and the base, the character of the blade margin and the epidermis. The study also high lighted the dimension of the blade (the length and the width), The morphological characteristics of the leaves have given a great Taxonomical value rather than the root and the stem. It also mentioned in this study the Inflorescences in general : the form and the diameter of the receptacle, the dimension of the inflorescence (length and width), and the characteristics of the phyllaries, the study of Achenes, the length of the beak, the character of the filaments that connected by the peak of the beak. The proper morphological characters of the Inflorescences made the contrastive among the characteristics of the Inflorescence in unique type. This great advantage is useful in the classification of the species. The anatomical study contains the study of the dermal tissue system that belong to the types under study, specially the stem, the leaf. It make clear the differences among the characters of the epidermis cells for the stem and the leaf (the upper and lower epidermises), It tackled the study of the Stomata at the leaf and the stem, and measure of the frequency at these members. It also discussed the fundamental and vascular tissue system of the root, the stem and the leaf through the study of their characteristics of the transversal sector. It is also studied the vascular bundles according to the thickness of the regions belonged to the xylem and phloem; the number of the xylem arms in each bundle and the number of the vascular elements in one xylem arm. The study involves the system of the veins into the leaf blade or that called (Venation), and the thickness of the veins and its course, the behavior of the vein's ends and the method of its contact with the leaf margin. In addition, the study dealt with the method of the joining veins the secondary by the primary and the third veins by the secondary. Thus, the study make clear the considerable advantages that distinguish the types each other. It is observed the characteristics of the leaf's venation is a constant character that depends upon to classify the species. At end of the study, It is used the results of the morphological and anatomical characteristics in comparison of the two tribes; Cardueae and Lactuceae that the studied species followed.

دراسة بعض مظاهر امراضية الخمج بالمتحولة النسيجية بين الاشخاص في مدينة سامراء == A Stud Y of Some Aspects of Entamoeba Histolytica Pathogenicity Among Peoples In Samarra

Author name: صلاح عبد حسن السامرائي
Supervisor name: ابراهيم شعبان داوود | حسين ساهر اسود
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: شملت هذه الدراسة فحص (540) عينة براز من المصابين بالاسهال والمراجعين لمستشفى سامراء العام وذلك خلال الفترة الممتدة بين 1/10/2007الى 1/6/2008 فحصت العينات بطريقة المسحة المباشرة المزدوجة للكشف عن الاطوار الخضرية والمتكيسة لطفيلي اميبا الزحار. واظهرت النتائ | The current study dealt with examination of (540) stool samples for patients with diarrhea who visited the General Hospital of Samarra during 1/10/2007 - 1/6/2008. The samples were examined by double wet direct smear method to identify the trophozoite and cyst stages of Entamoeba histolytica. The results showed that (112) were infected with amoebic dysentery. The total rate was (20.74 %). (262) are males with a rate of infection (19.84 %), whereas, the number of females was (278) with an infection rate (21.58 %). The study showed an increase rate of infection among the rural population, it was (25.71 %), whereas the urban population rate was (16.6 %). The study covered all ages beginning from less than a year to 75 years old. A highest rate of infection was recorded by (10 - 19) years old, it was (29.4%). The study also, found out that a highest rate of infection was during school months, particularly during November (27.5 %). Examinations of complete blood picture which included Hemoglobin concentration (Hb), packed cells volume (PCV), white blood cells count (WBCc), and erythrocyte sedimentation rate (ESR) were done on all patients infected with amoebic dysentery. The results were compared with those of healthy ones (Control Group). The results showed significant differences between the two groups. According to the significant one (P < 0.05), it recorded a decrease (16.9%) in the (PCV) and (25.1%) in the (Hb) and an increase (241.8%) in the (ESR) and (51.5%) in the (WBCc) for those ones infected with amoebic dysentery if compared with the control group. Clinical biochemistry examinations were performed, on blood sugar (Bs), electrolytes such as Sodium (Na+), Potassium (K+), Calcium (Ca++), Magnesium (Mg++), liver functions tests : total serum bilirubine(TSB), tranceaminase enzyme(GOT, GPT), alkaline phosphetase (AlP), total protein (TP) and albumin (Alb). The results showed that there are significant differences between persons infected with amoebic dysentery and the healthy ones according to the significant level (P < 0.05) in these examinations. The study showed a decrease(21.6%) in the (Bs) mean concentration, (16.8%) in the(K+) mean concentration, (5.8%) in the(Ca++) mean concentration, (4.5%)in the (Mg++) mean concentration, (10.5 %) in the (Na+) mean concentration , (17.2%) in the (TP) mean concentration and(12.2%) in the (Alb) mean concentration and increase (83.7%) in the (Alp) mean concentration, (52.1%) in the(TSB) mean concentration, (81.1%)in the (GPT) mean concentration, (59.9%)in the (GOT) mean concentration and(22.8%) in the (globulin) mean concentration in the persons infected with amoebic dysentery compared with the mean concentrations in healthy ones control group.

دراسة تاثير المستخلص المائي لنبات اللهانة على مستويات هرمونات الدرقية, الاجهاد التاكسدي وعدد من المعايير البيولوجية في الارانب == Study The Effect of Cabbage Aqueous Extract On The Thyroid Hormones Levels, Oxidative Stress And Some Biological Parameters In Rabbits

Author name: سرى سمير محمد الدوري
Supervisor name: زيد محمد مبارك المهداوي | صاحب جمعة عبد الرحمن
General topic: Biology
Specific topic: Zoology
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: هدفت الدراسة الحالية الى معرفة بعض تاثيرات المستخلص المائي لنبات اللهانة ((Brassica oleracea على مستويات هرمونات الغدة الدرقية الثايرونين ثلاثي اليود Triiodothyronine (T3) والثايروكسين (Thyroxine (T4والهرمون المحفز للدرقية Thyroid stimulating hormone (TSH | The aim of the present study was to investigate some effects of the aqueous extract of cabbage (Brassica oleracea L) on the Thyroid hormones concentrations Triiodothyronine T3, Thyroxine T4 and Thyroid stimulating hormone (TSH), Body weight rate, and some physiological and biochemical parameters such as the total count of leukocytes (WBCs), packed cell volume (PCV) and concentrations of Hemoglobin (Hb), Glucose, Total Cholesterol(TC), Triglycerides (TG), High Density Lipoprotein (HDL - C), Low Density Lipoprotein (LDL - C), Very Low Density Lipoprotein (VLDL - C), Phospholipids, Atherogenic indices levels, Uric acid, Total protein, Albumin, Globulin, Serum Creatinine Blood urea, glutathione(GSH), Malondialdehyde(MDA), Peroxynitrite radical(ONOO - ) Ceruloplasmin(Cp), Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) in sera of male rabbits exposed to oxidative stress induced by hydrogen peroxide (0.5%) with drinking water along the period of experiment (30) days, with comparison the effect of the cabbage extract with the effect of carbimazole (anti - thyroid drug) to induce Hypothyroidism.For the purpose of this study, preliminary experiments were carried out to find the most effective dose of the cabbage extract and this dose was (300 mg/kg body weight).Rabbits (8 - 10 months age) and (950 - 1560 gm weight) were determined randomly divided into five groups, each group includes five replicates These groups are : The control group, cabbage aqueous extract group, cabbage aqueous extract and H2O2 group, carbimazole drug group and H2O2 GroupThe results showed the following : 1 - Treatment the animals with cabbage aqueous extract caused significant decrease (P<0.05) in Hb, PCV and concentrations of T3 hormone, T4 hormone, TC, LDL - C, phospholipids, second atherogenic index, blood urea, serum creatinine, albumin, MDA, (ONOO - ) radical and Cp, While it caused significant increase in weights, TSH hormone, TG, VLDL - C, glucose, globulin and GSH compared with the control group. While no significant variations were observed in total (WBCs) and concentrations of AST, ALT, HDL - C, atherogenic index (first and third), uric acid and total protein compared with the control group. 2 - Treatment of rabbits with carbimazole drug showed significant decrease in levels of body weight, Hb, PCV, and concentrations of T3 hormone, HDL - C, blood urea, albumin, glucose, GSH and Cp. This treatment showed as well a significant increase in T4 hormone, TSH hormone, ALT, TG, uric acid, VLDL - C, serum creatinine and atherogenic indices levels, Whereas showed no significant variations in total (WBCs), AST, TC, LDL - C, phospholipids, total protein, globulin, MDA and (ONOO - ) radical compared with the control group. 3 - Compared with the control group the oxidative stress induced by H2O2 caused significant decrease in concentrations of Hb, T3 hormone, HDL - C, albumin, GSH and Cp, While this stress showed a significant increase in total (WBCs), T4 hormone, ALT, TG, VLDL - C, phospho - lipids, atherogenic indices levels, serum creatinine, glucose, MDA and (ONOO - ) radical. However no significant variations were observed in weights, TSH hormone, PCV, AST, TC, LDL - C, uric acid, blood urea, total protein and globulin compared with the control group.4 - The Treatment of rabbits under oxidative stress with cabbage extract showed significant decrease in Hb, PCV, T3 hormone, TC, LDL - C, phospholipids, atherogenic indices levels, blood urea, serum creatinine, albumin, GSH, MDA, (ONOO - ) radical and Cp comparison with the control group. While a significant increase was observed in weights and concentrations of T4 hormone, TG, HDL - C, VLDL - C, uric acid, glucose and globulin, While no significant variations showed in total (WBCs), TSH hormone, ALT, AST and total protein comparison with the control group.The study results revealed that the effect of cabbage aqueous extract was better than carbimazole effect as antithyroid and as antioxidant role by reduction the harmful effects of the free radicals through some improvement was observed in many physiological and biochemical activities in the body and may be used in protection and treatment many of pathological cases specially Hyperthyroidism, cancer and cardiovascular diseases.

التاثير الوقائي لعصير التفاح الاخضر والمستخلص المائي للزنجبيل في التقليل من التاثيرات الفسلجية والكيموحيوية للاشعة فوق البنفسجية والاشعة السينية في الجرذان == The Protective Effect of Green Apple Juice And Ginger Aqueous Extract In Lowering of The Physiological And Biochemical Effect From Ultraviolet Ray And X - Ray In Rats

Author name: عبير امين مصطفى الامين
Supervisor name: زيد محمد مبارك المهداوي | منى حسين جانكير
General topic: Biology
Specific topic: Zoology
Degree: Master
University: Tikrit University - College Of Science - Department Of Biology
Language: Arabic
University location: Salahaddin
First pages:
Abstract: صممت الدراسة الحالية لمعرفة التاثير الوقائي لعصير التفاح الاخضر (Malus domestica) والمستخلص المائي للزنجبيل (Zingiber officinale) من التاثيرات الضارة نتيجة التعرض للاشعة فوق البنفسجية والاشعة السينية في ذكور واناث الجرذان البيض لمدة ثلاثة ايام من خلال قيا | This study is designed to evaluate the protective effect of Green Apple (Malus domestica) juice and the aqueous extract of ginger (Zingiber officinale) against the side effect of Ultraviolet ray (UV) and X - ray in male and female albino rats for three days by examined some physiological and biochemical effects and histotoxicity in liver and kidney tissues. The rats with age (5 - 7) months and weight (250 - 350) g were divided into 16 groups (5 rats for each group) : group (1) control group received drinking tab water and ideal diet, group (2) Ultraviolet ray group only for half hour, group (3) Ultraviolet ray group only for one hour, group(4) UV group for half hour + green apple juice (2 ml/kg), group (5) UV group for half hour + green apple juice (4 ml/kg), group (6) UV group for one hour + green apple juice (2 ml/kg), group (7) UV group for one hour + green apple juice (4 ml/kg), group (8) UV group for half hour +ginger abstract (1000mg/kg), group (9) UV group for half hour +ginger abstract (2000mg/kg), group (10) UV group for one hour +ginger abstract (1000mg/kg), group (11) UV group for one hour +ginger abstract (2000mg/kg), group (12) X - ray group only, group (13) X - ray group+ green apple juice (2 ml/kg), group (14) X - ray group+ green apple juice (4 ml/kg), group (15) X - ray group+ ginger abstract (1000mg/kg), group (16) X - ray group+ ginger abstract (2000mg/kg).The study result showed that : 1. The radiation exposed rats by Ultraviolet ray for half hour and one hour and exposed group to X - ray only caused significant elevation in total count of leukocytes (WBCs), Platelets count, and in the level of blood sugar (glucose), Total Cholesterol, Triglyceride (TG), Low density lipoprotein - cholesterol (LDL - C), Very low density lipoprotein - cholesterol (VLDL - C), Phospholipids, Atherogenic indices levels, Urea, Creatinine, Malondialdehyde, Peroxy nitrate radical, and a significant decrease in Packed cell volume (PCV), Haemoglobin (Hb), count of Erythrocytes (RBCs), High density lipoprotein - cholesterol (HDL - C), total protein, Albumin, Globulin, Uric acid, Glutathione compared with control group.2. when the groups treated by green apple juice (2, 4 ml/kg) and the aqueous extract of ginger (1000, 2000 mg/kg) after that exposing rats to the radiation by Ultraviolet ray for half hour and the exposing groups to Ultraviolet ray for one hour only and the exposing groups to X - ray only showed significant reduction in total count of leukocytes (WBCs), Platelets count, and in the level of blood sugar (glucose), total Cholesterol, triglyceride (TG), Low density lipoprotein - cholesterol (LDL - C), Very low density lipoprotein - cholesterol (VLDL - C), Phospholipids, Atherogenic indices levels, Urea, Creatinine, Malondialdehyde, Peroxy nitrate radical, and a significant increase in Packed cell volume (PCV), Haemoglobin (Hb), count of erythrocytes (RBCs), High density lipoprotein - cholesterol (HDL - C), total protein, Albumin, Globulin, Uric acid, Glutathione compared with groups exposed to Ultraviolet ray only for half hour.3. The Exposing radiation of the rats groups by Ultraviolet ray and X - ray that lead to makes changes in tissues and many abnormalities in animals liver and kidney of these groups, in liver this included necrosis and degeneration in the hepatocytes and fibrosis and observed Atrophy hepatocytes and that lead to loss its normal shape and observed infiltration of Lymphocyte and many ceases of Central vein damage and degeneration of epithelial cells and that caused hemolysis and hemorrhage cases and appear of RBCs in tissue sections. While in Kidney the tissue abnormalities included necrosis and degeneration the epithelial cells of tubules and damage cases of glomerulus, tubeules and blood vesscles and degeneration of epithelial cells and that lead to hemorrhage cases in most area and combined with Infiltration of Lymphocyte.4. when the animals groups treated by green apple juice (2, 4 ml/kg) and the aqueous extract of ginger (1000, 2000 mg/kg) after that exposing rats to the radiation by Ultraviolet ray and X - ray, this abnormalities happened but more less, and observed the normals shaped in some area of tissues sections. The result showed the important and the ability of Green Apple juice (Malus domestica) and the aqueous extract of ginger (Zingiber officinale) in protect against the side effect of Ultraviolet ray and X - ray in male and female albino rats
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