اسم المؤلف:
عبير امين مصطفى الامين
اسم المشرف:
زيد محمد مبارك المهداوي | منى حسين جانكير
المستخلص:
صممت الدراسة الحالية لمعرفة التاثير الوقائي لعصير التفاح الاخضر (Malus domestica) والمستخلص المائي للزنجبيل (Zingiber officinale) من التاثيرات الضارة نتيجة التعرض للاشعة فوق البنفسجية والاشعة السينية في ذكور واناث الجرذان البيض لمدة ثلاثة ايام من خلال قيا | This study is designed to evaluate the protective effect of Green Apple (Malus domestica) juice and the aqueous extract of ginger (Zingiber officinale) against the side effect of Ultraviolet ray (UV) and X - ray in male and female albino rats for three days by examined some physiological and biochemical effects and histotoxicity in liver and kidney tissues. The rats with age (5 - 7) months and weight (250 - 350) g were divided into 16 groups (5 rats for each group) : group (1) control group received drinking tab water and ideal diet, group (2) Ultraviolet ray group only for half hour, group (3) Ultraviolet ray group only for one hour, group(4) UV group for half hour + green apple juice (2 ml/kg), group (5) UV group for half hour + green apple juice (4 ml/kg), group (6) UV group for one hour + green apple juice (2 ml/kg), group (7) UV group for one hour + green apple juice (4 ml/kg), group (8) UV group for half hour +ginger abstract (1000mg/kg), group (9) UV group for half hour +ginger abstract (2000mg/kg), group (10) UV group for one hour +ginger abstract (1000mg/kg), group (11) UV group for one hour +ginger abstract (2000mg/kg), group (12) X - ray group only, group (13) X - ray group+ green apple juice (2 ml/kg), group (14) X - ray group+ green apple juice (4 ml/kg), group (15) X - ray group+ ginger abstract (1000mg/kg), group (16) X - ray group+ ginger abstract (2000mg/kg).The study result showed that : 1. The radiation exposed rats by Ultraviolet ray for half hour and one hour and exposed group to X - ray only caused significant elevation in total count of leukocytes (WBCs), Platelets count, and in the level of blood sugar (glucose), Total Cholesterol, Triglyceride (TG), Low density lipoprotein - cholesterol (LDL - C), Very low density lipoprotein - cholesterol (VLDL - C), Phospholipids, Atherogenic indices levels, Urea, Creatinine, Malondialdehyde, Peroxy nitrate radical, and a significant decrease in Packed cell volume (PCV), Haemoglobin (Hb), count of Erythrocytes (RBCs), High density lipoprotein - cholesterol (HDL - C), total protein, Albumin, Globulin, Uric acid, Glutathione compared with control group.2. when the groups treated by green apple juice (2, 4 ml/kg) and the aqueous extract of ginger (1000, 2000 mg/kg) after that exposing rats to the radiation by Ultraviolet ray for half hour and the exposing groups to Ultraviolet ray for one hour only and the exposing groups to X - ray only showed significant reduction in total count of leukocytes (WBCs), Platelets count, and in the level of blood sugar (glucose), total Cholesterol, triglyceride (TG), Low density lipoprotein - cholesterol (LDL - C), Very low density lipoprotein - cholesterol (VLDL - C), Phospholipids, Atherogenic indices levels, Urea, Creatinine, Malondialdehyde, Peroxy nitrate radical, and a significant increase in Packed cell volume (PCV), Haemoglobin (Hb), count of erythrocytes (RBCs), High density lipoprotein - cholesterol (HDL - C), total protein, Albumin, Globulin, Uric acid, Glutathione compared with groups exposed to Ultraviolet ray only for half hour.3. The Exposing radiation of the rats groups by Ultraviolet ray and X - ray that lead to makes changes in tissues and many abnormalities in animals liver and kidney of these groups, in liver this included necrosis and degeneration in the hepatocytes and fibrosis and observed Atrophy hepatocytes and that lead to loss its normal shape and observed infiltration of Lymphocyte and many ceases of Central vein damage and degeneration of epithelial cells and that caused hemolysis and hemorrhage cases and appear of RBCs in tissue sections. While in Kidney the tissue abnormalities included necrosis and degeneration the epithelial cells of tubules and damage cases of glomerulus, tubeules and blood vesscles and degeneration of epithelial cells and that lead to hemorrhage cases in most area and combined with Infiltration of Lymphocyte.4. when the animals groups treated by green apple juice (2, 4 ml/kg) and the aqueous extract of ginger (1000, 2000 mg/kg) after that exposing rats to the radiation by Ultraviolet ray and X - ray, this abnormalities happened but more less, and observed the normals shaped in some area of tissues sections. The result showed the important and the ability of Green Apple juice (Malus domestica) and the aqueous extract of ginger (Zingiber officinale) in protect against the side effect of Ultraviolet ray and X - ray in male and female albino rats
