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تثبيط تعبير جينات fimH ,fimD ,fimC في بكتريا E. coli الممرضة للمجاري البولية باستعمال جسيمات ثنائي اوكسيد التيتانيوم النانوية المصنعة حيويا من المعززات الحيوية البكتيرية == Inhibition the Expression of fimC, fimD, fimH Genes in Uropathogenic E. coli Using TiO2 Nanoparticles Biosynthesized by Probiotics Bacteria

Author name: سوزان عبد الرحيم حسن
Supervisor name: سوسن ساجد الجبوري | جيهان عبد الستار سلمان
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Baghdad
Key words:
  • fimC
  • fimD
  • fimH Genes
  • Uropathogenic E. coli
  • TiO2 Nanoparticles
  • Probiotics Bacteria
  • biosynthesis
First pages:
Abstract: Summary ►Urinary tract infections (UTIs) are the commonest human bacterial infections and are responsible for substantial morbidity and mortality, resulting in increased healthcare costs. Specialized Escherichia coli strains referred to as Uropathogenic E. coli (UPEC) causes most UTIs. ►Total 120 isolates primarily identified as E.coli; were collected from (December 2018 to February 2019). Out of total collected isolates, 94/120 were included in this study and the contaminated bacteria or weak growth were excluded. The isolated pure colonies were re-identified using some standard morphological tests followed by genetically identification using housekeeping gene (HKG) 16S rDNA. The results confirm that all the isolates were definite as E. coli. ►The UPEC isolates showed their ability of adherence to uroepithelial cells. The aggregation ability of bacterial cells were appeared as dotes on the surface of epithelial cells. ►The antimicrobial susceptibility test (AST) was performed for 50 isolates under study. The highest resistance rate was concerning to Aztreonam; Cefazolin; Ceftazidime and Ceftriaxone (84%), Cefepime (82%), while the minimum resistant was for Imipenem; Ertapenem; Meropenem; Amikacin when it was (2%) beside that none of the isolates was able to resist Tigecycline. According to AST for fifty UPEC isolates; (88%) were multidrug resistance (MDR), while (2%) was extensively drug resistance (XDR) and (10%) were sensitive to all antibiotics thus they were non-drug resistant (NDR). ►All isolates were subjected to detect type1 fimbriae genes (fimA operon) by polymerase chain reaction using specific primers (designed in this study) followed by sequencing the amplified fragment and analyzed using Geneious prime software. The results showed that all the isolates (100%) were positive for fimA, fimI, fimC, fimD, fimG, fimH, while (96%) for fimB, fimF and (82%) for fimE. ►Raw milk samples were collected for probiotics bacteria isolation. All isolates were subjected to the cultural, microscopically, biochemical tests and Vitek2 system. Results indicated that six isolates of Lactococcus spp. Identified as: Lactococcus lactis ssp. lactis, in addition, 14 isolates of Leuconostoc spp. distributed among species as (8) isolates belonged to Leuconostoc mesenteroides ssp. cremoris (3) isolates belonged to Leuconostoc mesenteroides ssp. mesenteroides, (2) isolates identified as Leuconostoc pseudomesenteroides and only one as Leuconostoc citreum. ►All isolated probiotics bacteria were tested for Titanium dioxide (TiO2) nanoparticles production. The results showed that they had the ability to biosynthesize TiO2 NPs, biosynthesized TiO2 NPs were characterized using UVVis Spectroscopy; Atomic Force Microscope (AFM); X-ray Diffraction (XRD); Field Emission Scanning Electron Microscopy (FE-SEM) Energy dispersive Xray spectroscopy (EDX); and Fourier Transform-Infrared Spectroscopy (FTIR). The detected absorption spectrum with a wide-ranging absorption band in UV range and its cut off wavelength was (344-348) nm for all 20 probiotic isolates. ►The smoothness and roughness of all samples of biosynthesized TiO2 NPs was detected by AFM with smoothest sample for L. mesenteroides ssp. cremoris (Leu.4) as 1.137nm. XRD results indicated the formation of nano anatase crystals. ►The Scanning electron microscope images of biosynthesized TiO2 NPs biosynthesized by probiotics bacteria; that were consists of a uniform distribution of spherical shaped nanostructure crystals with a smallest size of (37.48) nm for L. citreum (Leu. 13). ►The EDX analysis of TiO2 NPs existing in medium which confirm that all the samples contained the elements (Ti, O), which again established that the biosynthesized NPs were pure TiO2. ►The determination of functional group of biosynthesized TiO2 NPs studied using FTIR. The main IR absorption peaks of TiO2 biosynthesized by probiotics bacteria were in range at (408- 445) cm-1; these peaks belonged to anatase Titania. ►The biosynthesized TiO2 NPs at concentrations (1000- 0.001) mg/mL were tested for its antibacterial activity against UPEC isolates, thus there was neither bactericidal effect against pathogenic bacteria nor been as minimum inhibitory concentration (MIC). ►There was no effect of temperature, pH and photocatalytic activation on antibacterial activity of biosynthesized TiO2 NPs against clinical UPEC isolates. ►Regardless of no growth inhibition activity showed on culture media after subjecting bacterial UPEC isolates to TiO2 NPs. There was a noticeable inhibitory effect of TiO2 NPs on the attachment ability of bacterial cells without killing them. The highest inhibition was recorded at the higher concentration of biosynthesized TiO2 NPs (1000 g/mL) with inhibition reached 92% and 90.3%, while the lowest inhibition were 52% and 54.8% at 250g/mL against E.coli74 and E.coli23, respectively. ►The effect of biosynthesized TiO2 NPs by probiotics bacteria and commercial TiO2 NPs was studied on the expression of fim genes. There was a subsequent change in the gene expression in term of folding and CT values when the expression of fimH, C, D genes start to decrease by increasing concentration of TiO2 NPs as compared with control and the maximum inhibitions was noticed by using 1000 g/mL concentration of the NPs specially against fimD.
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تقييم التاثير الوقائي لعقار Metformin ضد التضرر الوراثي الخلوي المستحث بواسطة الاشعة المؤينة == Assessment of protective effect of metformin drug against cytogenetic damage induced by ionizing radiation

Author name: علي نعمة سلمان
Supervisor name: فرحة عبد علي شفي | عبد الصاحب كاظم علي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: English
University location: Baghdad
Key words:
  • gamma ray
  • metformin
  • cytokinesis-block micronucleus
  • lymphocytes
Summary:
References:

متابعة بعض المؤشرات المناعية لمرضى كوفيد - 19 المتعافين == Follow up of Some Immunological Parameters of Recovered COVID - 19 Patients

Author name: سرى مسلم داود الاعرجي
Supervisor name: اقبال خضر الجوفي
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Baghdad
Key words:
  • علم الاحياء المجهرية
First pages:

دراسة تشخيصية لانواع دودة الارض في محافظة بغداد == Identification Study of Earthworm Species in Baghdad Governate

Author name: سرى باسل كمال محمد
Supervisor name: نبراس فالح جيجان | مجيد ارشيد سباح
General topic: Biology
Specific topic: Zoology
Degree: Doctorate
Language: English
University location: Baghdad
Key words:
  • علم الحيوان
First pages:

الارتباط البكتيري بين تسوس الاسنان والتهاب اللوزتين لدى عينة من الاطفال العراقيين == Bacteriological Correlation between Dental caries and tonsillitis in a sample of Iraqi Children

Author name: دنيا بدوي شبيب
Supervisor name: ايمان ناطق ناجي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Baghdad
Key words:
  • علم الاحياء المجهرية
First pages:

التاثير المضاد للبكتريا ولتكوين الغشاء الحيوي لانظيم التانيز المنقى من بكتريا Lactobacillus plantarum ضد البكتريا المسببة لاصابات المجاري البولية == Antibacterial and antibiofilm effect of tannase purified from Lactobacillus plantarum against urinary tract infections causative bacteria

Author name: داليا فلاح حسن
Supervisor name: ساهرة نصيف مسلم
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Baghdad
Key words:
  • علم الاحياء المجهرية
First pages:

العلاقة بين المحتوى البكتيري الطبيعي والعدوى ما بعد العمليات الجراحية باستخدام بعض انواع المطهرات == Association of Bacterial Microbiota and Post Operative Infections by using some antiseptic types

Author name: داليا عوني ادوار
Supervisor name: ايمان ناطق ناجي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Baghdad
Key words:
  • علم الاحياء المجهرية
First pages:

التاثيرات المضادة للبكتريا والغشاء الحيوي والمعدل المناعي للرامنوسين المنقى في نموذج اصابة الجهاز التنفسي بالزوائف الزنجارية == Antibacterial, antibiofilm and immunomodulation effects of purified rhamnocin in Pseudomonas aeruginosa respiratory infection model

Author name: حسين ستار جبار ربيعي
Supervisor name: لقاء حميد مهدي
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Baghdad
Key words:
  • الاحياء المجهرية
First pages:

دراسة العلاقة بين تعدد الاشكال لجين وبعض المعايير الكيمياوية الحيوية لمرضى تضخم الاطراف المصاحب لمرضى السكري العراقيين == Study the Association between IGFBP3 gene polymorphism and some biochemical parameters in Iraqi diabetic acromegaly patients

Author name: هند ماجد محسن
Supervisor name: رنا صباح جواد | نور ثائر طاهر
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: English
University location: Baghdad
Key words:
  • الحيوان
First pages:

التحري عن بعض المعايير الفسيولوجية لدى الاطفال المصابين باضطراب طيف التوحد في مدينة بغداد == Investigate of Some Physiological Parameters in Children With Autism Spectrum Disorder in Baghdad City

Author name: هاجر مؤيد ياسين
Supervisor name: اريج عباس زبون | عادل عبد الرحمن صديق
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: English
University location: Baghdad
Key words:
  • الحيوان
First pages:

التاثيرات المضادة للبكتريا والمضادة للسرطان للمارسيسين المنقى والجسيمات النانوية من بكتيريا Serratia marcescens == Antibacterial and Anticancer effects of Purified Marcescin and Nanoparticles from Serratia marcescens

Author name: زينب فرحان علي
Supervisor name: خولة جبر خلف | هند حسين عبيد
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Baghdad
Key words:
  • الاحياء المجهرية
First pages:

تقييم الفعالية ضد السرطان والمحللة لخثرة الدم لانزيم serratiopeptidase المنقى من بكتريا Serratia marcescens == Estimation of anticancer and thrombolytic activity of serratiopeptidase enzyme purified from Serratia marcescens

Author name: زهراء سالم بدر
Supervisor name: سوسن حسن عثمان | محمد فاضل ابو كسور
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Baghdad
Key words:
  • الاحياء المجهرية
First pages:

التاثيرات الفسلجية والنسيجية لدقائق السيليكا النانوية على اعضاء مختلفة في اناث الجرذ == Physiological and Histological Effects of Silica Nanoparticles on Different Organs of Female Rats

Author name: ريام صباح عبود
Supervisor name: نوري محمد لعيبي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: English
University location: Baghdad
Key words:
  • الحيوان
First pages:

تحضير وتوصيف جسيمات السيلينيوم النانوية وتحديد فعاليتها ضد البكتيرية وضد الغشاء الحيوي == Synthesis and Characterization of Selenium Nanoparticles and Determine their Antibacterial and Antibiofilm Activity

Author name: ريام خالد عطاالله
Supervisor name: محمد فاضل ابو كسور | اسامة عبد العزيز داخل
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Baghdad
Key words:
  • الاحياء المجهرية
First pages:

التحلل الحيوي لانزيم اللايبيز المنقى من Ralstonia mannitolilytica المعزولة من الترب الملوثة بمشتقات النفط == Biodegradation activity of lipase purified from Ralstonia mannitolilytica isolated from soils contaminated with oil derivatives

Author name: رؤى سعيد عبد الكريم
Supervisor name: عقيل محمد مجيد العزي | هديل كريم مسافر
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Baghdad
Key words:
  • الاحياء المجهرية
First pages:

دور الحامض النووي الرايبي المجهري (MiRNA125a) في امراضية التهاب الغدة الدرقية (Hashimoto’s) == The Role of miRNA - 125a In Pathogenesis of Hashimoto’s Thyroiditis

Author name: ساطع يوسف محمد
Supervisor name: حيدر جاسم محمد
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Baghdad
Key words:
  • علم الاحياء المجهرية
First pages:

دور الهرمونات السترويدية لدى النساء العراقيات ما قبل سن الياس والمصابات ببعض امراض المناعة الذاتية == Role of steroid hormones in Iraqi premenopause women with some autoimmune diseases

Author name: رند مشتاق طالب
Supervisor name: لمى قاسم علي | نبا احسان جواد
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: English
University location: Baghdad
Key words:
  • علوم الحياة
First pages:

مستويات الانترلوكين 6 و18 لدى مرضى تكيس المبايض والمصابات بالتهاب المسالك البولية البكتيري == Levels of Interleukin - 6 and Interleukin - 18 among Polycystic Ovarian Syndrome with Bacterial Urinary Tract Infections

Author name: رقية سعدي ابراهيم
Supervisor name: سوزان سعدي حسين | نهاد خلاوي تكتوك
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Baghdad
Key words:
  • علم الاحياء المجهرية
First pages:

تقييم كثافة كتلة العظم لدى مرضى النزف الوراثي == Evaluation of Bone Mass Density in Hemophilic Patients

Author name: رشا ابراهيم سلمان
Supervisor name: خالد مهدي صالح
General topic: Biology
Specific topic: Zoology
Degree: Doctorate
Language: English
University location: Baghdad
Key words:
  • علم الحيوان
First pages:

الفعالية المضادة للبكتريا والسرطان والاكسدة لحامض الكاليك المنتج من انزيم التانيز البكتيري المنقى == Antibacterial, anticancer and antioxidant activity of gallic acid produced by purified bacterial tannase

Author name: خالد عزيز هاشم الكناني
Supervisor name: ساهرة نصيف مسلم | سوسن حسن عثمان
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Baghdad
Key words:
  • الاحياء المجهرية
First pages:

ترابط مقاومة اللبتين مع مقاومة الانسولين وتاثيرهما على المعايير العصبية في مرضى السكري النوع الثاني والمصابين بالسمنة == Association of Leptin Resistance With InsulinResistance and their Effects on Neurological Parameters in Obese Type 2Diabetes Mellitus Patients

Author name: حوراء حسن جاسم
Supervisor name: جميله عيسى كاظم | يحيى دريعم صيهود
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: English
University location: Baghdad
Key words:
  • علم الحيوان
First pages:

التغيرات النسجية المرضية للمشيمة ومستويات بين ابيضاض لامصال الامهات والسائل الامنيوسي في حالات السائل المصبغ بالعقي == Placental histopathological changes and maternal serum and amniotic fluid interleukin levels in cases of meconium stained liquor

Author name: حنان فوزي سلمان
Supervisor name: سحر عبد الهادي الشرقي | عالية عصام محمود العبيدي
General topic: Biology
Specific topic: Zoology
Degree: Doctorate
Language: English
University location: Baghdad
Key words:
  • علم الحيوان
First pages:

دراسة قياسية شكلائية ونسجية قياسية وكيموحيوية مقارنة لبعض اعضاء الجهاز الهضمي بين طيور Psittacula krameri وElanus caeruleus البالغة == Morphometrical, Histometrical and Biochemical Comparative Study of some Organs of Digestive System between birds adult Elanus caeruleus and Psittacula krameri

Author name: جيهان محمود رجب
Supervisor name: صباح عبد الحميد عبد الرحمن | سحر عبد الهادي الشرقي
General topic: Biology
Specific topic: Zoology
Degree: Doctorate
Language: English
University location: Baghdad
Key words:
  • علم الحيوان
First pages:

التاثير المضاد للبكتريا والسرطان لانزيم elastase الحر والمقيد المنتج من بكتريا Klebsiella pneumoniae المعزولة من مصادر سريرية == Antibacterial and Anticancer Effects of Free and Immobilized Elastase Produced by Clinical Isolates of Klebsiella pneumoniae

Author name: جواد نوار كحيط الموسوي
Supervisor name: نهاية حكمت زكي
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Baghdad
Key words:
  • الاحياء المجهرية
First pages:

تقييم فعالية بعض مركبات الايض الثانوي المعزولة ضد Spirogyra aequinoctialis من الطحالب بعض الفطريات == Evaluation the Activity of Some Secondary Metabolic Compounds Isolated from Spirogyra aequinoctialis Against Some Fungi

Author name: ثامر حميد خليل
Supervisor name: احمد ساهي دويش
General topic: Biology
Specific topic: Plant
Degree: Master
Language: English
University location: Baghdad
Key words:
  • علم النبات
First pages:
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