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دراسة جزيئية للـ Bacteriocine المنتج من قبل بكتريا lactobacillus salivarius == Molecular Study of Bacteriocine Produce By Lactobacillus Salivarius

اسم المؤلف: ايمان حمزة محمد

اسم المشرف: احلام نعيم كاظم الياسين

الموضوع العام: علوم الحياة

السنة: 2013

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة الكوفة - كلية التربية للبنات - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: النجف

الصفحات الاولى:

المستخلص: هدفت الدراسة الحالية التحري عن قابلية عزلات بكتريا Lactobacillus salivarius على انتاج البكتريوسين ودوره في تثبيط فعالية بعض العزلات البكتيرية السالبة لصبغة غرام. جمعت 155 عينة من مصادر سريرية مختلفة شملت 20عينة مسحات مهبلية، 8 عينة مسحات اللعاب، 6عينة حل | The aime of study detecting the a bility of Lactobacillus salivarius to produce bacteriocin and determine it’s role in inhibition the activity of some Gram negative bacteria. Atotal of 155 sample from different clinical source has been collected during the period from October - 2012 to March - 2013, these include 20 samples from vaginal smear, 8 samples from saliva and 6 samples from human milk.The results of primary isolation, Laboratory diagnosis and biochemical test showed that 34 isolates has bee identified as L.salivarius.Ahigh precent of isolation has been carried out from human milk which was 40%. The ability of all isolates to produce gelatinase and biofilm has been detected and the results revealed the ability of some isolates to produce gelatinase and biofilm. Ahigh percent of gelatinase production has been detected in L.salivarius isolated from milk sample (50%), while alow percent of gelatinase production was 0% in L.salivarius isolated from saliva. The results of optical density of biofilm produced by L.salivarius isolates from milk which was 100% higher than it in isolates from vaginal and saliva which was 85% and 75% respectively. All isolates shows antibacterial effect against some Gram negative bacteria, the diameter of inhibition as between (2 - 8), the results showed the ability of 4 selective isolates of L.salivarius to produce bacteriocin in which the Rf valve of precipitate bacteriocin was (0.42 - 0.6) compard with leaky bacteriocin hich was (0.57 - 0.8), also leaky bacteriocin as more activity against G - ve bacteria than precipitate bacteriocin. The result of plasmid profile in L.salivarius revealed the presence of megaplasmid in all isolate except one which had one megaplasmid and one small plasmid. Bacterial conjugation between L.salivarius that produce bacteriocin and had megaplasmid which used as adonor cell with E. coli Jm 109 as a recipient cell showed transferring of megaplasmid from donor to recipient cell and the transconjugant cells showed high anti - bacterial effect due to bacteriocin production against G - ve bacteria. The result of amplification of Sal P gene encoding salivracin P by PCR confirmed that all isolate had no Sal P gene in there genetic materials which revealed that these isolates had another type of bacteriocin rather than Salivracin P.