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دراسة جزيئية للـ Bacteriocine المنتج من قبل بكتريا lactobacillus salivarius == Molecular Study of Bacteriocine Produce By Lactobacillus Salivarius
Author name:
ايمان حمزة محمد
Supervisor name:
احلام نعيم كاظم الياسين
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
Abstract:
هدفت الدراسة الحالية التحري عن قابلية عزلات بكتريا Lactobacillus salivarius على انتاج البكتريوسين ودوره في تثبيط فعالية بعض العزلات البكتيرية السالبة لصبغة غرام. جمعت 155 عينة من مصادر سريرية مختلفة شملت 20عينة مسحات مهبلية، 8 عينة مسحات اللعاب، 6عينة حل | The aime of study detecting the a bility of Lactobacillus salivarius to produce bacteriocin and determine it’s role in inhibition the activity of some Gram negative bacteria. Atotal of 155 sample from different clinical source has been collected during the period from October - 2012 to March - 2013, these include 20 samples from vaginal smear, 8 samples from saliva and 6 samples from human milk.The results of primary isolation, Laboratory diagnosis and biochemical test showed that 34 isolates has bee identified as L.salivarius.Ahigh precent of isolation has been carried out from human milk which was 40%. The ability of all isolates to produce gelatinase and biofilm has been detected and the results revealed the ability of some isolates to produce gelatinase and biofilm. Ahigh percent of gelatinase production has been detected in L.salivarius isolated from milk sample (50%), while alow percent of gelatinase production was 0% in L.salivarius isolated from saliva. The results of optical density of biofilm produced by L.salivarius isolates from milk which was 100% higher than it in isolates from vaginal and saliva which was 85% and 75% respectively. All isolates shows antibacterial effect against some Gram negative bacteria, the diameter of inhibition as between (2 - 8), the results showed the ability of 4 selective isolates of L.salivarius to produce bacteriocin in which the Rf valve of precipitate bacteriocin was (0.42 - 0.6) compard with leaky bacteriocin hich was (0.57 - 0.8), also leaky bacteriocin as more activity against G - ve bacteria than precipitate bacteriocin. The result of plasmid profile in L.salivarius revealed the presence of megaplasmid in all isolate except one which had one megaplasmid and one small plasmid. Bacterial conjugation between L.salivarius that produce bacteriocin and had megaplasmid which used as adonor cell with E. coli Jm 109 as a recipient cell showed transferring of megaplasmid from donor to recipient cell and the transconjugant cells showed high anti - bacterial effect due to bacteriocin production against G - ve bacteria. The result of amplification of Sal P gene encoding salivracin P by PCR confirmed that all isolate had no Sal P gene in there genetic materials which revealed that these isolates had another type of bacteriocin rather than Salivracin P.
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دراسة بكتريولوجية - وراثية البكتريا Acinetobacter baumannii المعزولة من مستشفيات مدينة النجف الاشرف == Bacteriological And Genetic Study Of Acinetobacter Baumannii Isolated From Hospitals In Al-Najaf Al-Ashraf City
Author name:
سحر محمد جواد
Supervisor name:
احلام كاظم نعيم الياسين
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
English
University location:
Najaf
First pages:
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فاعلية عسل النحل الطبيعي ضد الزوائف الزنجارية والعنقوديات الذهبية المخمجة للجروح في الجرذان المهقاء == Efficiency of natural bee honey against Pseudomonas aeruginosa and Staphylococcus aureus associated with infected wounds in albino rats
Author name:
رغد محمد علي جاسم الدعمي
Supervisor name:
جميل جري يوسف الحميداوي
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
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دراسة مناعية لحالات العقم الناتجة عن الاصابات البكتيرية لدى الرجال في محافظة النجف الاشرف == Immunological Study of Infertility Cases in Male which Associated with Bacterial Infection in AL-Najaf AL-Ashraf Governorate
Author name:
قيس خضير عبد العباس الحدراوي
Supervisor name:
سهام جاسم محسن الكعبي | احسان عيدان عبد الكريم السيمري
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
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استخدام تقنية التفاعل التضاعفي التسلسلي للتحري عن بكتريا Gardnerella vaginalis في حالات الخمج المهبلي البكتيري غير المتخصص == Using of Polymerase Chain Reaction Technique for Detection of Gardnerella vaginalisIsolates In Non-Specific Bacterial vaginosis
Author name:
حنان خالد دايخ الظالمي
Supervisor name:
احلام كاظم نعيم الياسين
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
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دراسة بكتريولوجية ووراثية للمكورات العنقودية الذهبية المقاومة للمثيسيلين المعزولة من مستشفيات مدينة النجف
Author name:
ميعاد كاظم علي الخضيري
Supervisor name:
علي محسن المحنة
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
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دراسة التاثيرات السمية لحبوب الحنطة الملوثة بالفطرين Aspergillus flavus وAspergillus niger في الجهاز التناسلي لاناث الجرذ الابيض وامكانية السيطرة عليها في المخزن == Study of the toxic effects of the fungi Aspergillus flavus and Aspergillus niger in the reproductive system of females of Albino rats and the ability to control it in the store
Author name:
عذراء حرجان محسن
Supervisor name:
سامي عبد الرضا علي الجميلي
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
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دراسة الفعالية التضادية لمستخلصات اوراق نباتي اليوكالبتوس Eucalyptus camaldulensis D. والزعتر Thymus vulgaris L. في جرثومة Staphylococcus aureus خارج جسم ذكور الفئران البيض وداخله == of white mice Eucalyptus camaldulensis D.and Thymus vulgaris L.on Staphylococcus aureus in vitro and in males Study of antibiosis activity of extracts of
Author name:
سهير عبد الكريم حبيب الرماحي
Supervisor name:
عباس جواد الصراف | ثامر خضير مرزه
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
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دراسة بكتيريولوجية لمسببات تجرثم الدم الهوائية في النجف == BACTERIOLOGICAL STUDY ON EAROBIC CAUSES OF BACTERAEMIA IN NAJAF
Author name:
جنان محمد حسين فيروز علي
Supervisor name:
محمد صبري عبد الرزاق السعيد
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
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التحري عن حامض الكولانيك في بعض العزلات المرضية لبكتريا Klebsiella pneumoniae == Investigation of Colanic Acid by Some Isolates Klebsiella pneumoniae
Author name:
غصون محمد علي وداي العطية
Supervisor name:
محمد صبري عبد الرزاق السعيد
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
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امكانية تصنيع مبيد احيائي من الفطر Trichoderma harzianum Rifai لمكافحة مرض تعفن البذور وموت البادرات في الحنطة == Possibility of manufacturing Bio-fungicide from Trichoderma harzianum Rifai fungus to control seed decay and damping-off on Wheat
Author name:
صباح لطيف علوان
Supervisor name:
سامي عبد الرضا الجميلي
General topic:
Biology
Specific topic:
Microbiology
Degree:
Doctorate
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
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التحري عن بعض انزيمات البيتالاكتاميز في العزلات السريرية لبكتريا الزوائف الزنجارية في مدينة النجف == Investigation of some B-Lactamases in Clinical Isolates of Pseudomonas aeruginosa in Najaf City
Author name:
الهام جواد كاظم بلال
Supervisor name:
علي محسن المحنة
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
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دراسة بكتريولوجية ومناعية للبايوسين المستخلص من الزائفة الزنجارية المعزولة محليا وتاثيره على الخلايا السرطانية خارج وداخل الجسم الحي == Bacteriological & Immunological Study on Pyocin Extracted from Local Isolate of Pseudomonas aeruginosa and its Effect on Cancer Cell in vivo and in vitro
Author name:
ماجدة مالك متعب
General topic:
Biology
Specific topic:
Microbiology
Degree:
Doctorate
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
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دراسة تاثير مستخلصات نباتي الياس L. Myrtus communis والثوم Allium sativum L. في بكتريا Pseudomonas aeruginosa خارج وداخل الجسم الحي == Study of effect of extracts of Myrtus communis L. and Allium sativum L. on Pseudomonas aeruginosa in vitro and in vivo
Author name:
نبراس يحيى عبد الله السلامي
Supervisor name:
عباس جواد الصراف | ثامر خضير مرزه
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
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دراسة ميكروبيولوجيه لالتهاب عنق الرحم بين النساء في مدينة النجف الاشرف == Microbiological Study of Cervicitis among Women in Najaf AL-Shraf City
Author name:
ابتهال اسماعيل عبد الله الشمرتي
Supervisor name:
جعفر كاظم نعمة الموسوي
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Kufa - College Of Education For Girls - Department Of Biology
Language:
Arabic
University location:
Najaf
First pages:
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