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دراسة جزيئيه لبعض الاصابات المعوية بالاوالي والفطريات الطبية == Molecular Study Of Certain Protozoal and Fungal Infections

Author name: عباس غافل عباس الوائلي
Supervisor name: بهيجة عبيس الخالدي | معاني ناجي الشمري
General topic: Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Qadisiyah
First pages:

تعدد الاشكال الجينية ل A Disintegrin and Metalloprotease 33 وبعض المؤشرات المناعية في الربو القصبي == A Disintegrin and Metalloprotease 33 Gene polymorphism and Some Immunological Markers in Bronchial Asthma

Author name: مريم عطية خزعل
Supervisor name: منال محمد كاظم | علي طالب الدامرجي
General topic: Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Qadisiyah
First pages:

تاثير المركبات الفينولية على حيوية الاشكال المسوطة لطفيلي الشمانيا المدارية خارج الجسم الحي == Effects of Phenolic Compounds on the Viability of Leishmania tropica promastigotes : A comparative in vitro study

Author name: رؤى حميد عبد المجيد الصباغ
Supervisor name: معاني ناجي الشمري | باسم ارحيم محمد
General topic: Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Qadisiyah
First pages:

دور تعدد الاشكال الجينية لبروتامين 1 وبعض العوامل المناعية في الازواج العقيمين == The Role of Protamine 1 Gene Polymorphism and Some Immunological Factors in Infertile Couples

Author name: ثائر علي حسين
Supervisor name: منال محمد كاظم | تغريد صالح القزويني
General topic: Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Qadisiyah
First pages:

التحري عن بعض جينات البيتا - لاكتاميز واسعة الطيف في بكتيريا اشريشيا القولون والكليبسيلا المعزولة من مرضى سرطان القولون والمثانة في مدينة الديوانية == Detection of Some Extended Spectrum Beta - Lactamases Genes in Escherichia coli & Klebsiella spp Isolated from Colon and Bladder Cancer Patients in Al - Diwaniya City

Author name: اثير فلاح كاظم الفتلاوي
Supervisor name: عدنان حمد الحمداني | عادل موسى الركابي
General topic: Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Qadisiyah
First pages:

دراسة حدوث الاصابة بفيروس المضخم للخلايا وداء المقوسات الكونيدية على حالات النساء المجهظات في محافظة الديوانية == Occurrence Study of Human Cytomegalovirus and Toxoplasmosis Infection among Miscarriages Women in Al - Diwaniya Province

Author name: عقيل عباس كريم الخفاجي
Supervisor name: حمادي عبطان الهلالي | معاني ناجي الشمري
General topic: Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Qadisiyah
First pages:

تاثير المركبات الفينولية والتيربينات على حيوية الرؤوس اللاولية لدودة المشوكات الحبيبية == Effects of Phenolic Compounds and Terpenoid on the Viability of Echinococcus granulosus Protoscolices

Author name: حيدر اسكندر فليح
Supervisor name: معاني ناجي الشمري | باسم ارحيم محمد
General topic: Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Qadisiyah
First pages:

دراسة الاصابة ببعض الجراثيم المسببة لالتهاب الاذن الوسطى وعلاقتها مع الحالة المناعية == A Study of Some Frequently Isolated Bacteria from Otitis Media in Association with Immunological Status

Author name: علاوي جواد العبيدي
Supervisor name: حمادي عبطان الهلالي | راهي كلف الياسري
General topic: Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Qadisiyah
First pages:

دراسة تعدد الطرز الوراثية لجين Toll - like Receptor - 4 وبعض السايتوكينات في المرضى الذين يعانون من عدوى المسالك البولية المتكررة == Study of Toll - like Receptor - 4 Gene Polymorphism and some Cytokines Profile in Patients with Recurrent Urinary Tract Infection

Author name: احمد هادي دخيل
Supervisor name: حمادي عبطان الهلالي | علي نعيم سلمان
General topic: Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Qadisiyah
First pages:

تاثير مستخلص الكركم الخام على التعبير الجيني لمورثة البروتين المنظم للغشاء الحيوي لجرثومة Streptococcus mutans المعزولة من التجويف الفمي == Effect of Curcumine Crude Extract On The Gene Expression of Biofilm Regulatory Protein A (Brp A) In Streptococcus Mutans Isolated From Oral Cavity

Author name: نزار احمد شهاب
Supervisor name: عدنان حمد عبيد الحمداني | عدنان حمد عبيدالحمداني
General topic: Medicine
Specific topic: Microbiology
Degree: Master
Language: English
University location: Qadisiyah
First pages:
Abstract: جمعت 100 مسحة فموية من مرضى تسوس الاسنان وامراض ما حول الاسنان الذين تراوحت اعمارهم ما بين 15 - 65 سنة ومن كلا الجنسين والذين راجعوا المركز التخصصي لطب الاسنان في الديوانية للمدة من كانون الاول 2012 الى اذار 2013 لغرض قياس التعبير الجيني لمورثة البروتين ا | A total of 100 oral swabs were collected from patients suffering from dental caries and priodontal infections, ranging from 15 - 65 years old of both genders whom admitted to the Al - Diwaniyah Teaching Special Center of Dentistry within the period from December 2012 to March 2013, in order to quantify the gene expression of biofim regulatory protein (Brp A) in Streptococcus mutans isolates by using the Real Time polymerase chain reaction. According to morphological, cultural characteristics and biochemical tests by using VITEK - 2 system, 34/84(40.47%) isolates of S.mutans were isolated and identified among streptococcus species and the isolation percentages were 26/50(52%) in males and in females were 24/50(48%), while the age group (15 - 25 years old) was the most affected than the other groups. The statistical analysis showed no significant differences among the interaction between gender and age groups. Molecular detection of 16s rRNA gene was amplified to confirm the diagnosis of S.mutans isolates, where all isolates gave an amplicon size (151) bp, in addition to, the DNA sequencing was done in order to identify the nitrogen bases sequences and phylogeny of local isolates in comparison with the global isolates (Spain, Japan, Korea, USA, Canada, India), results revealed that the local isolates of S.mutans had a similarity percent (90%) with Spain isolates.Furthermore, the susceptibility of isolates to a variety of antibiotics have been investigated. It has been found that isolates had multiresistance to erythromycin(88.2%),ampicillin(76.4%),amoxiclave(70.5%),tetracycline(61.7%), nalidixic acid and amoxicillin (58.8%), chloramphenicol (55.8%). The statistical analysis showed a significant differences among tested antibiotics. Biofilm formation was investigated phenotypically by using tissue culture plate with crystal violet staining for resistant S.mutans isolates, the results revealed that all isolates were high biofilm former, furthermore, the effect of ethanolic extraction (50%) of curcumine in growth inhibition and biofilm reduction of S. mutans was assayed. The results showed an effect of curcumine in comparison with chlorhexidine(2%) as a control.The gene expression of biofilm regulatory protein A(Brp A) was quantified in comparison with housekeeping gene 16s rRNA as a reference gene of biofilm S.mutans by using reverse transcriptase polymerase chain reaction (RT - PCR). The results revealed that the expression (relative&absolute) of Brp A gene is decreased in the test treatment (S.mutans with 1% glucose+ 50% curcumine) in comparison with control (S.mutans grown in 1% glucose only).In conclusion, the present study established the role of Brp A gene in biofilm formation
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