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العزل والتعبير الجيني لجينات BabA في العزلات السريرية للبكتيريا الملوية البوابية Helicobacter pylori والمعالجة بالمعزز الحيوي لخميرة Saccharomyces boulardii == Isolation and Gene expression of BabA Gene in Clinical Isolates of Helicobacter pylori Treated with Probiotic Yeast Saccharomyces boulardii
Author name:
عامر علي حمادي
Supervisor name:
محسن هاشم رسن | حسن محمود موسى
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Doctorate
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
First pages:
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التنميط المتسلسل متعدد المواقع لبكتريا اشرشيا القولون متعددة المقاومة للمضادات الحياتية والمعزولة من التهاب المجاري البولية == Multi - locus Sequence Typing of Multi - drug Resistant Escherichia coli Isolated from Urinary Tract Infections
Author name:
اية احمد كمال العامري
Supervisor name:
عصام سلمان العزاوي | ظفر نجم العكيلي
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
First pages:
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انتاج, تنقية وتوصيف انزيم الاسباراجينيز من العصيات المعزولة محليا == Production, Purification and Characterization of L - asparaginase from Locally Isolated Bacillus sp.
Author name:
نور رياض ابراهيم
Supervisor name:
نضال سهيل زبار
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
First pages:
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التحري الجزيئي عن العناصر المنتقلة المشفرة للمقاومة لمضادات الحياة في عزلات سريرية لبكتريا Streptococcus pneumoniae == Molecular Detection of Transposable Elements Conferring to Antibiotic Resistance in Clinical Isolates of Streptococcus pneumoniae
Author name:
دعاء حمود ادعير ال منشد
Supervisor name:
حميد مجيد جاسم
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology - Department Of Biotechnology
Language:
English
University location:
Baghdad
First pages:
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التاثير السمي الخلوي والسمي الجيني لـ Lantadene A المنقى المحمل بواسطة توليف دقائق الذهب النانوية على خطوط الخلايا السرطانية == Cytotoxic and Genotoxic Effect of Purified Lantadene (A) Loaded by Synthesized Gold Nanoparticles on Tumor Cell Lines
Author name:
نور ضياء جعفر
Supervisor name:
علي زيد الصفار | عماد عبد الحسين يوسف
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
First pages:
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عزل وتشخيص مناعي نمطي للخلايا الجذعية المزنشيمية من الحبل السري البشري == Isolation and Immunophentypic Characterization of Mesenchymal Stem Cells from Human Umbilical Cord
Author name:
سالي مازن اكرم
Supervisor name:
زهراء كامل زيدان
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
First pages:
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عزل وتشخيص عاثي المكورات العنقودية الذهبية وتقييم نشاطه المضاد للبكتيريا == Isolation and Characterization of Staphylococcus aureus Bacteriophage and Evaluation of Its Antibacterial Activity
Author name:
عارف عباس غايب
Supervisor name:
ابراهيم عبد الله احمد | حامد خلف احمد
General topic:
Biology
Specific topic:
Microbiology - Viruses
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology - Department Of Biotechnology
Language:
English
University location:
Baghdad
Key words:
phage Rih21
phage genome analysis
bacteriophage
S. aureus
Staphylococcus aureus phage Rih21
staphylococcus aureus
bacteriophage
novel bacteriophage
Staphylococcus phage Rih21
MRSA
First pages:
Abstract:
The antimicrobial therapy, in the long term, is expected to become ineffective against Staphylococcus aureus (S. aureus) due to its ability to produce new and more virulent strains. Bacteriophages are known to fight bacteria for a very long time and have the ability to adapt to the developing bacteria. In this study, 129 clinical specimens were collected from different places in Baghdad and from different clinical sources during the period from October 13, 2020, to January 26, 2021. Only 45 isolates showed cultural (mannitol salt and blood agar) and biochemical (catalase, coagulase and oxidase test) characteristics that were specific to S. aureus. The identity of S. aureus isolates was further confirmed by using specific primers for amplification and sequencing of the S. aureus thermonuclease (nuc) gene, and the blast result of sequences in the NCBI database was 99% to 100% identical to S. aureus species. Also, the S. aureus isolates were tested for sensitivity of different types of antibiotics, and results revealed that: all S. aureus isolates demonstrated high levels (100%) of resistance against the cefoxitin, penicillin, and ceftazidime. It also showed intermediate levels of resistance against tetracycline 55.56% (25/45 isolates), and azithromycin 46.67% (21/45 isolates), and the lowest levels of resistance was against the erythromycin 44.44% (20/45 isolates), rifampin 17.78% (8/45 isolates), trimethoprim/sulfamethoxazole 17.78% (8/45 isolates), clindamycin 15.56% (7/45 isolates), gentamicin 13.33% (6/45 isolates) and ciprofloxacin 11.11% (5/45 isolates). also found that no antibiotics tested in this study were able to kill all S. aureus isolates, therefore all isolates tested in this study were classified as Methicillin-resistant Staphylococcus aureus. From the hospital waste-water, a novel bacteriophage was isolated and characterized. The enrichment method was used for isolation of Staphylococcus aureus bacteriophage and six rounds of purification based on the plaque assay were used to purify a single type of bacteriophage against S. aureus clinical isolates. According to characterization properties, this bacteriophage belongs to the Siphoviridae family and can form a halo around the phage plaque. The maximum bacteriophage titer was recorded at 37°C and a pH of 7.2. The bacteriophage titers were decrease at 45°C, 50°C, and 55°C, until completely lost its activity at 60°C. This bacteriophage was completely inactivated at highly acidic (3, 4) and basic (10, 11) pH values and lost part of its activity at pH 5, 6, 8, and 9. This bacteriophage had a 44,789 bp linear double-strand DNA genome, and within the genome sequence, there are 61 genes, all of which are encoded into proteins. Although this bacteriophage does not have any virulence factors or antimicrobial resistance genes and had specific lytic activity against some antimicrobial resistance S. aureus clinical isolates. All the features mentioned above about this bacteriophage could make it a valuable antibacterial choice against antimicrobial-resistant S. aureus clinical isolates.
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التباين الوراثي لجين SLC2A9 وعلاقته ببعض المظاهر الكيموحيوية في عينة من المرضى العراقيين المصابين بداء النقرس == Genetic polymorphism in SLC2A9 gene and its association with some biochemical aspects in a sample of Iraqi gout patients
Author name:
يسر رضا ممدوح النقشلي
Supervisor name:
وليد حميد يوسف | عبد الواحد شمخي جابر
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
First pages:
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التحري الجزيئي عن العناصر المنتقله المصحابة لمضادات الحياة في بكتيريا Escherichia coli المعزولة من التهابات المجاري البولية وحساسيتها للعاثيات == Molecular Detection of Transposable Elements Associated with Antibiotic Resistance in UTI Isolates of Escherichia coli and Phage Susceptibility
Author name:
شهد رعد مزعل
Supervisor name:
حسن محمد نايف
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Doctorate
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
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الفعالية السمية الخلوية والضد ميكروبية لجزيئات الفضة النانوية المخلقة من مستخلص الميرامية Salvia Officinalis على بعض العزلات المرضية == Cytotoxic and Antibacterial Activity of Silver Nanoparticles Synthesized by Salvia officinalis Extract on Some Pathogenic Bacteria
Author name:
سارة مهند ياسين عزيز
Supervisor name:
رقية محمد ابراهيم | اسماء علي حسين
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
First pages:
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العلاقة بين المؤشرات الكيموحيوية والتباين الوراثي في جين اللاديبونكتين مع حدوث متلازمة التكيس المبيضي في العراقيات البدينات == Association of Biochemical Markers and Adiponectin gene polymorphism with the incidence of Polycystic Ovarian Syndrome in Iraqi Obese women
Author name:
سارة ابراهيم حاشوش
Supervisor name:
اسماء علي حسين | صلاح مهدي الجلبي
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Doctorate
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
First pages:
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التوصيف الجزيئي والكيموحيوي لانزيم البيتالاكتميز المنتج من العزلة المحلية لبكتريا المكورات العنقودية == Molecular and Biochemical Characterization of β- lactamase Produced from Local Isolate of Staphylococcus aureus
Author name:
رقية ماجد حسن
Supervisor name:
مها حميد عبد الله | غازي منعم عزيز
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
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estimation the levels of serum cystatin c protein levels as a Biomarker of Renal Function in some Iraqi Patients with Renal Disease
Author name:
Ruaa Jawad Shunaba Almuhana
Supervisor name:
Maha H.Abdullah
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
First pages:
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العلاقة بين التباين الوراثي ل LCS1 و LCS6 والتعبير الجيني لجين KRAS مع سرطان الثدي في عينة من النساء العراقيات == Association between genetic Polymorphism of LCS1 and LCS6 and KRAS Gene Expression with Breast Cancer in a sample of Iraqi Women
Author name:
رؤيا هادي خزعل الحداد
Supervisor name:
حميد مجيد جاسم | وئام عبد الفتاح صالح
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Doctorate
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
First pages:
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تاثير تعدد الاشكال الوراثي لجين مستقبل هرمون الحليب في استحداث سرطان الثدي وفرط افراز هرمون الحليب في بعض النساء العراقيات == Effect of genetic polymorphism of prolactin receptor gene on initiation of breast cancer and hyperprolactinemic in some Iraqi women
Author name:
قبس عبد الوهاب محمد
Supervisor name:
رحاب صبحي رمضان
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology - Department Of Biotechnology
Language:
English
University location:
Baghdad
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التخلص من الغشاء الحيوي لبكتريا klebsiella pneumoniae المسببة لاصابات الحروق والجروح بالمعاملة المشتركة للمعالجات الحيوية والمضادات الحياتية == Eradication of Biofilm Produced by Klebsiella pneumoniae Causing Burns and Wounds Infections by Combinations of Probiotics and Antibiotics
Author name:
عمر نذير عباس
Supervisor name:
عبد الواحد باقر الشيباني | احمد علي مهاوش
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology - Department Of Biotechnology
Language:
English
University location:
Baghdad
First pages:
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مرافقة تعدد الاشكال الوراثي في جين pd-1 مع الاصابة بداء السكري مع النوع الاول في عينة من المرضى العراقيين == Association of PD-1 Gene polymorphism with the incidence of the Type 1 Diabetes Mellitus in a sample of Iraqi patients
Author name:
علا عماد خضير
Supervisor name:
حميد مجيد جاسم | ابراهيم عبد الله احمد
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology - Department Of Biotechnology
Language:
English
University location:
Baghdad
First pages:
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العلاقة بين الفيروسات المعوية والسكري من النوع الاول في الاطفال == The Correlation Between Enteroviruses and Type I Diabetes Mellitus in Children
Author name:
نور حسن جمعة
Supervisor name:
حسن محمد نايف | عباس مهدي رحمة
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
تاثير مثلية الدنا والتعبير الجيني لجينات H19 و SNRPN و LINE- 1 على الرجال المصابين بقلة النطف == Impact of DNA Methylation and Gene Expression of H19, SNRPN and LINE -1 Genes on Oligospermic Men Infertility
Author name:
هاله كمال محسن القزاز
Supervisor name:
سلوى جابر العوادي
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Doctorate
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
دراسة تاثير التباين الوراثي (G894T) في جين تصنيع اكسيد النيتريك المرتبط بتغير مساوى البيروكسي نتريت كعامل خطورة للاصابة بداء السكري من النوع الثاني لعينة من المرضى العراقيين == Assessment the effect of Genetic Polymorphism (G894T) in Nitric Oxide Synthase Gene Associated with Peroxynitrite Level Variation as a risk factor in Type 2 Diabetes Mellitus in a sample of Iraqi Patients
Author name:
علياء حسن بوهان
Supervisor name:
سامية خليل محمود
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
تعدد الانماط الاليلية لجين مستقبل فيتامين D وتقييم بعض الجوانب الكيموحيوية لمرض داء السكري النوع الثاني == Genetic Polymorphism of Vitamin D Receptor Gene and Evaluation of Some Biochemical Aspects in Type 2 Diabetes Patients
Author name:
احمد ضياء حسين التركي
Supervisor name:
حسين اللامي
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
الكشف عن الجينات المسؤولة عن تكوين الاغشية الحيوية لبكتريا Escherichia coli المعزولة من مرضى التهابات المجاري البولية == Detection of Genes Involved in Biofilm Formation by Escherichia coli Isolated from Patients Suffering from Urinary Tract Infection
Author name:
عدنان ملوح جابر
Supervisor name:
حسن عبد الهادي حسين
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
تحفيز الخلايا الجذعية الصباغية الماخوذة من بصيلات الشعر البشرية الى خلايا صباغية وظيفية في الزجاج == Induction of Human Hair Follicle Melanocyte Stem Cells into Functional Melanocyte: In vitro Study
Author name:
ايهاب قيس علي
Supervisor name:
شهلاء مهدي صالح
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Doctorate
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
تقييد انزيم الاسباراجينيز على دقائق الذهب النانوية لاستخدامه في التطبيقات الحيوية == Immobilization of L - asparaginase on Gold Nanoparticles for Biomedical Applications
Author name:
علي غازي حمدي الدليمي
Supervisor name:
علي زيد الصفار | غسان محمد سليمان
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Doctorate
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
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الفعالية السمية لانزيم الكلوتامنيز المنتج من عينات المكورات العنقودية الذهبية المرضية == Cytotoxic Action of L - Glutaminase Enzyme Produced from Staphylococcus aureus Clinical Isolate
Author name:
مصطفى غزاي الجحيشي
Supervisor name:
اسماء علي حسين
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Nahrain University - College of Biotechnology
Language:
English
University location:
Baghdad
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